Abrégé

In an embodiment, a bioprocessing method includes securing a vessel containing a fluid to a platform capable of pivoting about a first axis, heating the fluid in the vessel while the platform is in a static, substantially upright position to facilitate a first bioprocessing procedure within the vessel. The method further includes pivoting the platform and vessel about the first axis to create a rocking motion to facilitate a second bioprocessing procedure within the vessel.

Classes IPC  ?

• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• B01F 31/23 - Mélange du contenu de récipients indépendants, p.ex. de tubes à essai en faisant pivoter les récipients autour d'un axe
• C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
• C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p.ex. par des compteurs de colonies
• C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur

Abrégé

A sparger assembly (100) for a bioprocessing system (10) includes a base (126) and a plurality of spargers (130) connected to the base, each sparger including a plurality of pores, the plurality of spargers each have a generally cylindrical shape. Each of the plurality of spargers includes a sidewall and a top, which define the cylindrical shape, the sidewall and the top each include a plurality of pores. The pores of the sidewall can be arranged around a circumference of the sidewall at an array of heights. Ridges may also be located on the sidewall above a respective array of pores.

Classes IPC  ?

• B01F 23/231 - Mélange de gaz avec des liquides en introduisant des gaz dans des milieux liquides, p.ex. pour produire des liquides aérés par barbotage
• B01F 23/233 - Mélange de gaz avec des liquides en introduisant des gaz dans des milieux liquides, p.ex. pour produire des liquides aérés en utilisant des agitateurs entraînés munis d’éléments d'agitation complètement immergés
• B01F 33/453 - Mélangeurs magnétiques; Mélangeurs avec agitateurs à entraînement magnétique en utilisant des éléments d'agitation supportés ou suspendus
• B01F 35/513 - Récipients souples, p.ex. sacs supportés par des conteneurs rigides
• C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p.ex. avec agitateur à turbine
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• B01F 101/44 - Mélange d'ingrédients pour la microbiologie, l'enzymologie, la culture in vitro ou la manipulation génétique

Abrégé

An apparatus (30) for reducing a minimum working volume of a stirred tank (12) includes a body (32) having upper (46) and lower (48) surfaces, and an exterior profile shaped to closely fit within an interior of the stirred tank. The body including an interior portion (34) extending through the body (32) from the upper surface to the lower surface, the interior portion configured to receive and partially surround an impeller (200) within the stirred tank, while allowing rotation of the impeller to agitate a fluid in the vessel when in use. The body further including an opening in a side surface of the body, the opening extending into the interior portion and configured to facilitate removal of fluid (60) from the vessel when the stirred tank is in use, and to be removed from the stirred tank via a drain.

Classes IPC  ?

• B01F 33/453 - Mélangeurs magnétiques; Mélangeurs avec agitateurs à entraînement magnétique en utilisant des éléments d'agitation supportés ou suspendus
• B01F 35/513 - Récipients souples, p.ex. sacs supportés par des conteneurs rigides
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie

Abrégé

A vessel (24) includes an interior volume (25) containing a liquid, a spiral shaft (28) located within the interior volume, and a rotatable impeller (30). The impeller has an aperture (38) that receives the spiral shaft and allows the impeller to travel bidirectionally along the spiral shaft. When the impeller travels along the spiral shaft, the impeller rotates axially about the spiral shaft to agitate a liquid in the interior volume.

Classes IPC  ?

• B01F 27/07 - Agitateurs caractérisés par leur montage sur l’arbre
• B01F 31/40 - Mélangeurs avec mécanismes à secousses, oscillants ou vibrants avec un agitateur rotatif oscillant axialement
• B01F 33/453 - Mélangeurs magnétiques; Mélangeurs avec agitateurs à entraînement magnétique en utilisant des éléments d'agitation supportés ou suspendus
• B01F 35/513 - Récipients souples, p.ex. sacs supportés par des conteneurs rigides
• C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p.ex. avec agitateur à turbine
• B01F 101/44 - Mélange d'ingrédients pour la microbiologie, l'enzymologie, la culture in vitro ou la manipulation génétique

Abrégé

Provided is a membrane transfer cassette for electroblotting, the cassette comprising a first support panel and a second support panel, at least a region of one or each of the first and second support panels having an interlocking pattern of polygonal apertures. The membrane transfer cassette is particularly useful when electroblotting with large format gels as the design of the cassette increases the exposed surface area between the gel and the membrane while also enhancing the strength of the cassette to support large gels but is also advantageous for any size of gel.

Classes IPC  ?

• B01D 57/02 - Séparation, autre que la séparation de solides, non entièrement couverte par un seul groupe ou sous-classe, p.ex. par électrophorèse
• G01N 27/447 - Systèmes utilisant l'électrophorèse

Abrégé

The present invention provides for simple and rapid filtering of biological samples, whereby a sample can be analyzed in the same device or a different device. In one preferred example, a membrane filter (12) that is particularly useful for the filtration of samples comprising viruses along with other biological materials that need be separated from the viruses is used. A lateral flow device (10) incorporating a filter membrane/membrane filter (12) is also disclosed.

Classes IPC  ?

• B01D 63/08 - Modules à membranes planes
• B01D 69/10 - Membranes sur support; Supports pour membranes
• G01N 33/543 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/558 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet utilisant la diffusion ou la migration de l'anticorps ou de l'antigène

Abrégé

Methods and apparatus for scaling in bioprocess systems are disclosed. An example apparatus for bioprocess scaling includes at least one memory to store instructions, and processor circuitry to execute the instructions to identify at least one operating parameter of a target bioreactor, determine an upper boundary and/or a lower boundary defining a design space for at least one bioreactor process parameter to match at least one target parameter range based on the at least one operating parameter, simulate changes in the at least one target parameter range based on an adjustment to the upper boundary and/or the lower boundary in the design space, and configure the target bioreactor using output obtained from the adjustment to the upper boundary and/or the lower boundary to identify a match between the at least one target parameter range and a user-based input of a target bioprocess parameter value.

Classes IPC  ?

• C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p.ex. fermenteurs commandés automatiquement

Abrégé

Porous liquid-filtering membranes (102) are provided having a boundary region (303) substantially surrounding a pore region (302) and having greater tear resistance than the pore region (302). In various aspects, a liquid-filtering tear-resistant porous polymeric membrane (102) comprises a pore region (302) comprising a plurality of pores having a minimum dimension of less than (100) microns and a ring-shaped tear- resistant boundary region (303).

Classes IPC  ?

• B01D 61/14 - Ultrafiltration; Microfiltration
• B01D 67/00 - Procédés spécialement adaptés à la fabrication de membranes semi-perméables destinées aux procédés ou aux appareils de séparation
• B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétés; Procédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
• B01D 71/64 - Polyimides; Polyamide-imides; Polyester-imides; Polyamide-acides ou précurseurs similaires de polyimides

Abrégé

A sampling system includes a graduated sampling chamber configured for fluid connection to a sample source, a pump device configured for fluid connection with the sampling chamber, and a sterile air filter intermediate the pump device and the sampling chamber, wherein the pump device is selectively actuatable to draw a volume of fluid from the sample source into the sampling chamber.

Classes IPC  ?

• C12M 1/26 - Inoculateur ou échantillonneur
• G01N 33/48 - Matériau biologique, p.ex. sang, urine; Hémocytomètres

Abrégé

A sterile foam breaking system (14, 42, 52) includes a foam collector (20) having an opening (26), configured to be disposed in a source (12) which generates foam. The sterile foam breaking system (14, 42, 52) further includes a non-contact type suction unit (23) coupled to the foam collector (20). The non-contact type suction unit (23) is configured to transfer the foam via the opening (26) of the foam collector (20) and break a portion of the foam to generate a first quantity of liquid droplets. The sterile foam breaking system (14, 42, 52) additionally includes a foam breaking unit (28, 44, 54) coupled to the non-contact type suction unit (23). The foam breaking unit (28, 44, 54) is configured to receive remaining portion of the foam and the first quantity of liquid droplets and break the remaining portion of the foam to generate a second quantity of liquid droplets.

Classes IPC  ?

• B01D 19/02 - Dispersion ou prévention de la mousse
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie

Abrégé

The present invention relates to a sample processing bag (10) for use in a tissue disaggregation apparatus (200) for disaggregation of tissue therein. A clamp assembly 60 comprising a base support (70) and a clamp mechanism (72) is configured to retain the sample processing bag (10) adjacent to the base support (70) during a tissue disaggregation operation such that action of the tissue disaggregation apparatus 200 acts on the sample processing bag (10) to disaggregate any tissue therein.

Classes IPC  ?

• C12M 1/33 - Désintégrateurs
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie

Abrégé

An apparatus (100) for managing gas bubbles (50) in a bioprocessing system (10) includes a body portion (110) having a underside surface (112), and an opening (114) in the body portion (110). The body portion (110) is configured for placement within a bioreactor vessel (12) such that the underside surface (112) of the body portion (110) is disposed in a liquid within the bioreactor vessel (12). The underside surface (112) of the body portion (110) is configured to divert rising gas bubbles (50) in the liquid towards the opening (114).

Classes IPC  ?

• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie

Abrégé

A biocompatible polymeric membrane includes pores (106) defined between two material layers, where the first membrane material layer (101) includes strips, and the second membrane material (104) binds to each of the plurality of first membrane material layer strips (101) includes a plurality of windows (105) exposing each of the first membrane material strips (101). The biocompatible polymeric filtration membrane comprises pores (106) defined by uniform passages defined by the first membrane material layer strips (101) and the second membrane material layer (104) within each window (105).

Classes IPC  ?

• B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétés; Procédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
• B01D 69/12 - Membranes composites; Membranes ultraminces
• B01D 71/64 - Polyimides; Polyamide-imides; Polyester-imides; Polyamide-acides ou précurseurs similaires de polyimides

Abrégé

Methods and apparatus for bioprocess monitoring are disclosed. An example apparatus includes a controller to monitor a first bioprocess instrument, a data logger to collect data for the first bioprocess instrument, the collected data including data collected while the first bioprocess instrument is transferred from a first location to a second location, a configurator to configure the first bioprocess instrument to operate in a first mode at the first location and in a second mode at the second location, the first or second mode determined based on a type of processing at the first or second location, and a user interface to display the collected data to a user, the collected data including real-time bioprocess monitoring data, the controller to adjust a setting of the first bioprocess instrument based on the monitoring data, the monitoring data used to maintain controlled environmental conditions within a vessel of the instrument.

Classes IPC  ?

• C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p.ex. fermenteurs commandés automatiquement
• G05B 15/00 - Systèmes commandés par un calculateur
• G06N 3/00 - Agencements informatiques fondés sur des modèles biologiques
• G16B 40/00 - TIC spécialement adaptées aux biostatistiques; TIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p.ex. extraction de connaissances ou détection de motifs
• G16C 20/10 - Analyse ou conception des réactions, des synthèses ou des procédés chimiques

Abrégé

A mixing system (10) includes a container (20) for containing a fluid, and an agitator assembly (100) disposable in the container (20) for mixing the fluid. The agitator (100) includes an extensible shaft (110) and at least one impeller (116, 118) connected to the extensible shaft (110).

Classes IPC  ?

• B01F 7/00 - Mélangeurs à agitateurs tournant dans des récipients fixes; Pétrins
• B01F 13/08 - Mélangeurs magnétiques
• B01F 15/00 - Accessoires pour mélangeurs
• C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p.ex. avec agitateur à turbine

Abrégé

The invention relates to the field of microcarrier perfusion culture of adherent cells. Specifically, the present invention relates to a high-density microcarrier retention device for perfusion culture of adherent cells, a microcarrier perfusion culture system for adherent cells containing the device, and methods of use thereof. The retention device of the present invention includes a sedimentation chamber, a pipeline connected to a bioreactor, a microcarrier retention filter membrane, a liquid backflushing device, an air backflushing device, a peristaltic pump and a pipeline connected to a receiver. The device has high efficiency in promoting the separation of microcarriers from cell culture medium and is helpful for perfusion culture of adherent cells and microcarriers. The retention device makes the culture volume in the bioreactor more flexible, can perform perfusion culture of 20%-100% of the maximum culture volume of the bioreactor, and the retention device can be linearly amplified according to the amplification of the bioreactor volume.

Classes IPC  ?

• C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie

Abrégé

A component management apparatus for a bioprocessing system includes a frame having a plurality of segments, including at least a first segment and a second segment pivotably connected to the first segment such that at least the second segment is movable between a closed position and an open position, and at least one mounting bracket connected to the frame for connection of a bioprocess component.

Classes IPC  ?

• C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie

Abrégé

The bioprocessing perfusion system (10) includes a bioreactor (12) and a feed flow path (14). A first tangential flow filter (16) is coupled to the bioreactor (12) via the feed flow path (14) and a second tangential flow filter (18) is coupled to the bioreactor (12) via the feed flow path (14). The first tangential flow filter (16) is a microfiltration-type filter and the second tangential flow filter (18) is an ultrafiltration-type filter. The first tangential flow filter (16) and the second tangential flow filter (18) are further coupled to a receiving unit (58) via the permeate flow path (60). The first tangential flow filter (16) and the second tangential flow filter (18) are further coupled to the bioreactor (12) via the retentate flow path (46). A control unit (82) is communicatively coupled to the first feed control device (42), the second feed control device (44), the feed drive unit (40), the first permeate control device (64), the second permeate control device (66), the first retentate control device (48), and the second retentate control device (50).

Classes IPC  ?

• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p.ex. fermenteurs commandés automatiquement
• B01D 61/14 - Ultrafiltration; Microfiltration

Abrégé

A device (30) includes a base connector (32) having an opening (33) and an impeller connector (64) coupled to the base connector (32). The impeller connector (64) has a through-passage (66) aligned with the opening (33) of the base connector (32). Further, the device (30) includes a flexible tube (34) having a first end (36) and a second end (40), where the first end (36) of the flexible tube (34) is coupled to the impeller connector (64). Furthermore, the device (30) includes a seal component (38) and an impeller (42) coupled to the second end (40) of the flexible tube (34). Additionally, the device (34) includes an enclosure (46) disposed enclosing the impeller (42), the flexible tube (34), the impeller connector (64), and the base connector (32).

Classes IPC  ?

• B01F 7/16 - Mélangeurs à agitateurs tournant dans des récipients fixes; Pétrins avec agitateurs tournant autour d'un axe vertical
• B01F 15/00 - Accessoires pour mélangeurs
• B01F 15/06 - Systèmes de chauffage ou de refroidissement
• B01F 3/04 - Mélange, p.ex. dispersion, émulsion, selon les phases à mélanger de gaz ou de vapeurs avec des liquides
• B01F 7/00 - Mélangeurs à agitateurs tournant dans des récipients fixes; Pétrins

Abrégé

The present invention relates to a bioprocess bag (118) comprising a bag wall defining an enclosed volume for holding biomaterials. The bag wall comprises at least one inlet port (142) and at least one outlet port (146). The bioprocess bag (118) also comprises a tube structure (400) comprising a first opened-end proximate the bag wall and a second distal end, the tube extending into the enclosed volume, and the tube structure (400) comprising a reinforced portion (402) proximate the first opened-end.

Classes IPC  ?

• B01L 3/02 - Burettes; Pipettes
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p.ex. avec agitateur à turbine
• C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces

Abrégé

Disclosed is a method for monitoring cell density during cell expansion resulting from a cell culture process in a bioreactor comprising the steps of: a) cultivating cells in a bioreactor culture chamber according to a cell culture process having cell culture parameters; b) during said process, introducing cell culture fluid inputs and generating waste materials; c) determining the intensity of volatile organic compounds (VOCs) and their chemical species in the waste materials; and d) estimating the density or population of cells in the bioreactor based on said determination.

Classes IPC  ?

• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p.ex. par des compteurs de colonies