Abrégé

Disclosed is a method for monitoring cell density during cell expansion resulting from a cell culture process in a bioreactor comprising the steps of: a) cultivating cells in a bioreactor culture chamber according to a cell culture process having cell culture parameters; b) during said process, introducing cell culture fluid inputs and generating waste materials; c) determining the intensity of volatile organic compounds (VOCs) and their chemical species in the waste materials; and d) estimating the density or population of cells in the bioreactor based on said determination.

Classes IPC  ?

• C12Q 1/06 - Détermination quantitative
• C12N 5/071 - Cellules ou tissus de vertébrés, p.ex. cellules humaines ou tissus humains
• C12N 5/0783 - Cellules T; Cellules NK; Progéniteurs de cellules T ou NK
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p.ex. par des compteurs de colonies
• C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p.ex. fermenteurs commandés automatiquement
• C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des micro-organismes viables

Abrégé

A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.

Classes IPC  ?

• C07K 1/14 - Extraction; Séparation; Purification
• C07K 1/04 - Procédés généraux de préparation de peptides sur des supports
• C07K 17/02 - Peptides immobilisés sur, ou dans, un support organique
• C07K 17/12 - Cellulose ou ses dérivés
• C12N 9/00 - Enzymes, p.ex. ligases (6.); Proenzymes; Compositions les contenant; Procédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 9/96 - Stabilisation d'une enzyme par formation d'un adduct ou d'une composition; Formation de conjugaisons d'enzymes
• C07H 3/06 - Oligosaccharides, c. à d. saccharides comportant de trois à cinq radicaux saccharide liés entre eux par des liaisons glucosidiques

Abrégé

Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3' end and a 5' end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10 C below the reaction temperature. The amplification is effected under isothermal condition.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C12Q 1/6844 - Réactions d’amplification d’acides nucléiques
• C12Q 1/686 - Réaction en chaine par polymérase [PCR]

Abrégé

The present invention relates to the field of cell biology, in in particular to methods for differentiating pluripotent stem cells. The invention provides methods for differentiating primate pluripotent stem cells into cells of all three germinal layers. In particular, methods for differentiating primate pluripotent stem cells into the definitive endoderm are provided.

Classes IPC  ?

• C12N 5/073 - Cellules ou tissus embryonnaires; Cellules fœtales ou tissus fœtaux
• C12N 5/071 - Cellules ou tissus de vertébrés, p.ex. cellules humaines ou tissus humains
• C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des micro-organismes viables

Abrégé

A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

Classes IPC  ?

• C12N 15/09 - Technologie d'ADN recombinant

Abrégé

Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a bilogical sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.

Classes IPC  ?

• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p.ex. pour test de réaction en chaîne par polymérase [PCR]
• C12Q 1/6844 - Réactions d’amplification d’acides nucléiques
• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN

Abrégé

Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of circulating nucleic acids extracted from blood is provided. Kits for performing the disclosed methods are also provided.

Classes IPC  ?

• C12Q 1/6862 - Réaction en chaine par ligase [LCR]
• C12Q 1/6844 - Réactions d’amplification d’acides nucléiques
• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

A method of amplifying RNA template is provided. The method comprises reverse-transcribing a ribonucleic acid (RNA) template to form a cDNA using a first reaction mixture comprising RNA template, at least one primer capable of hybridizing to the RNA template, a reverse transcriptase and deoxynucleoside triphosphates (dNTPs); and amplifying the cDNA to form an amplified product using a second reaction mixture comprising at least one strand displacement DNA polymerase, at least one inosine-containing primer and a nuclease that is capable of nicking DNA 3' to an inosine residue of the primer. The method is accomplished under an isothermal condition without denaturing the cDNA template. A method of quantifying RNA template in a sample and a method of detecting RNA template in a sample are also provided. Kits for amplifying deoxynucleic acid (DNA) from ribonucleic acid (RNA) template are also provided.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides

Abrégé

The present disclosure generally relates to solid matrices for the extraction, stabilization, and storage of nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for extracting, collecting, and recovering nucleic acids from the solid compositions are also described.

Classes IPC  ?

• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
• G01N 1/34 - Purification; Nettoyage

Abrégé

Methods and kits for efficient amplification of nucleic acids are provided. The disclosure generally relates to methods and kits for nucleic acid amplification of target nucleic acids of interest. The methods described herein promote the synthesis of the target nucleic acid (i.e., template nucleic acid) by reducing the production of undesirable primer-dimer structures and chimeric nucleic acid products during the amplification process by using novel modified primers.

Classes IPC  ?

• C12P 19/34 - Polynucléotides, p.ex. acides nucléiques, oligoribonucléotides
• C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p.ex. acides nucléiques

Abrégé

The present invention relates to solid supports that are used for the storage and further processing of biological materials. The invention is particularly concerned with solid supports which have at least one surface coated with a chemical that enhances the recovery of the biological material from the support. Methods of preparing and using the solid supports are also described.

Classes IPC  ?

• G01N 33/96 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir un étalon de contrôle du sang ou du sérum
• C08L 51/06 - Compositions contenant des polymères greffés dans lesquels le composant greffé est obtenu par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone; Compositions contenant des dérivés de tels polymères greffés sur des homopolymères ou des copolymères d'hydrocarbures aliphatiques ne contenant qu'une seule liaison double carbone-carbone
• G01N 33/543 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/544 - Support organique

Abrégé

The present invention relates to solid supports that are used for the storage and further processing of biological materials. The invention is particularly concerned with solid supports which have at least one surface coated with a chemical mixture that enhances the recovery of the biological material from the support. Methods of preparing and using the solid supports are also described.

Classes IPC  ?

• G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p.ex. utilisation de bandes de papier indicateur
• A61B 10/00 - Autres méthodes ou instruments pour le diagnostic, p.ex. pour le diagnostic de vaccination; Détermination du sexe; Détermination de la période d'ovulation; Instruments pour gratter la gorge
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes

Abrégé

A container holding a freeze-dried material comprising a biological sample is provided. The container includes a chamber with an upper portion and a lower portion. The freeze-dried material is located in the lower portion and the container comprises a physical structure protruding inward from the wall to inhibit freeze-dried material for moving to the lower portion. The freeze-dried material defines a first cross-section and the physical structure comprises a stop extending inwards from an internal wall of the chamber, defining a second cross-section. The stop defines a boundary between the upper portion and the lower portion. The stop comprises a movable insert, the movable insert being moveable within the chamber to vary the boundary.

Classes IPC  ?

• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes

Abrégé

Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate.

Classes IPC  ?

• G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
• G01N 1/38 - Dilution, dispersion ou mélange des échantillons
• G01N 1/40 - Concentration des échantillons
• G01N 30/60 - Préparation de la colonne