In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
05 - Produits pharmaceutiques, vétérinaires et hygièniques
Produits et services
Reagents for medical diagnostic use in polymerase chain
reaction (PCR) to detect and analyze nucleic acids in
laboratories, hospitals, point-of-care, and direct consumer
applications, and the associated accessory products, namely,
medical diagnostic test media in the form of test plates and
96 well plates for use in polymerase chain reaction (PCR) to
detect and analyze nucleic acids in laboratories, hospitals,
point-of-care and direct consumer applications for use in
medical genetic testing.
A method of genotyping includes applying a sample solution including a plurality of copies of a sample polynucleotide to an array of sensors. The sample polynucleotide includes a region associated with an allele. The method further includes measuring using a plurality of sensors of the array of sensors a characteristic of the region of the plurality of copies of the sample polynucleotide and determining using a computational circuitry and the measured characteristics a statistical value indicative of the allele.
A fluidic device for performing isotachophoresis comprise a substrate defining a fluidic network comprising a plurality of inlet channels, the outlets of the inlet channels meeting at a juncture, an intermediate channel extending from the juncture and fluidically coupled to the outlets of the plurality of inlet channels, a first reservoir at the juncture and fluidically coupled to the inlet channels and the intermediate channel, a second reservoir fluidically coupled to the intermediate channel at a location downstream of the juncture, wherein the inlet channels, the collection channel, and the intermediate channel are co-planar, and wherein the first reservoir and second reservoir are configured to produce substantially equal pressure heads. Methods of loading the fluidic device for isotachophoresis can be sequentially through the various inlet channels.
A filter bag assembly includes: a flexible first sheet; a flexible second sheet overlying and secured to the first sheet so that a compartment is formed therebetween; a porous filter sheet disposed between the first sheet and the second sheet so as to divide the compartment into a first compartment and a second compartment; a first port secured to the second sheet so as to communicate directly with the first compartment; and a second port secured to the second sheet so as to communicate directly with the second compartment, the second port being spaced apart from the first port. The porous filter sheet is disposed so that fluid entering the first compartment through the first port must pass through the filter sheet before entering the second compartment or exiting through the second port.
A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.
Methods and systems for data distribution are described herein. In one aspect, a method can include receiving, by at least one worker node (WN) 110 of a plurality of WNs of a data distribution system, a job definition from a job manager, wherein the job definition comprises a set of processes to be performed on a set of data request; sending, by the WN 110 and to an extended binary access manager (BAMEx) 140, a request for the data; receiving, by the WN 110 and from the BAMEx 140, the data based on the request for the data; performing, by the WN 110, one or more processes according to the job definition; generating, by the WN 110, a set of results files comprising results of at least one of the performed one or more processes; and sending, by the WN 110, the set of results files to the BAMEx 140.
The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p.ex. séquençage par hybridation [SBH]
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
A reagent solution includes water, a nucleotide, and tris(2-carboxyethyl)phosphine in a range of 0.5 μM to 1000 μM. The reagent solution can further include a non-ionic surfactant in an amount of 0.001% to 1% or a biocidal agent in an amount of 0.001% to 1%. The reagent solution can include salts, such as sodium chloride or magnesium sulfate.
A method, composition and kit for determining the presence or absence of Salmonella sp., Shigella sp./Enteroinvasive Escherichia coli (EIEC), and Campylobacter sp. in a sample, comprising producing an amplicon by subjecting a reaction mixture including the sample and five primer pairs to reaction conditions suitable to amplify targeted nucleic acids. The five primer pairs include primer pair A that specifically hybridizes with a portion of Campylobacter coli genome, primer pair B that specifically hybridizes with a portion of Campylobacter jejuni genome, primer pair C that specifically hybridizes with a portion of Campylobacter upsaliensis genome, primer pair D that specifically hybridizes with a portion of Salmonella sp. genome, and primer pair E that specifically hybridizes with a portion of Shigella sp./EIEC genome; and determining the presence or absence of Campylobacter sp., Salmonella sp., and/or Shigella sp./Enteroinvasive Escherichia coli (EIEC) in the sample based on the amplicon.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
The present disclosure provides for compounds of Formula (I),
its corresponding compounds of Formula (II)
or salts thereof and their use as fluorogenic pH sensors.
Methods and system are described for normalizing and creating correction factors for measurements in a fluorometer. To ensure that measurements across multiple channels can be properly compared, some kind of calibration must be done. The same calibrant can be run across all the channels and fluorescence measured. The fluorescence of one channel can be chosen as a reference. A correction factor can be calculated for each channel and used for an extended period of time, possibly even the lifetime of the instrument.
21.
DIBENZOXANTHENE QUENCHERS, USES, AND METHODS OF PREPARATION
The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described.
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p.ex. transfert d’énergie de résonance
An aseptic cell culture system including a cell culture device is disclosed. The cell culture device includes an interior chamber having a first opening extending through a wall defining a part of the interior chamber, and a first aseptic port. The first aseptic port includes a first coupling end and a second coupling end, the first coupling end extending through the first opening and integrated with the wall. Further, the first aseptic port includes a fluid pathway in fluid communication with the interior chamber and extends between the first coupling end and the second coupling end. The second coupling end includes a connecting component configured to allow one or more of a fluid line and a filter to be connected to the first aseptic port.
Methods for determining genotypes of structural variants in a sample genome, may include: amplifying nucleic acid sequences at targeted locations in the sample genome by a panel targeting a plurality of structural variant marker to generate sequence reads; mapping the sequence reads to a modified reference genome to produce aligned sequence reads, wherein the modified reference genome includes a wild-type target region and a structural variant target region; for each structural variant marker, determining a read count for a wild-type allele and a read count for a structural variant allele; determining a probability for each possible genotype, wherein the possible genotypes include a homozygous wild-type genotype, a heterozygous genotype and a homozygous structural variant genotype; and selecting the genotype with a maximum probability value to provide an estimated genotype corresponding to the structural variant marker of the sample.
Provided herein are compositions, methods and uses that relate to an easy, accurate and reliable dual or duplex assay that determines both protein and nucleic acid amounts in a viral preparation in a single container and further determines full versus empty virion content.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
25.
HIGH EFFICIENCY, SMALL VOLUME NUCLEIC ACID SYNTHESIS
The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
C25B 9/70 - Assemblages comprenant plusieurs cellules
C25B 11/04 - PROCÉDÉS ÉLECTROLYTIQUES OU ÉLECTROPHORÉTIQUES POUR LA PRODUCTION DE COMPOSÉS ORGANIQUES OU MINÉRAUX, OU DE NON-MÉTAUX; APPAREILLAGES À CET EFFET Électrodes; Leur fabrication non prévue ailleurs caractérisées par le matériau
C25B 15/02 - Commande ou régulation des opérations
26.
POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.
LIFE TECHNOLOGIES HOLDINGS PTE LIMITED (Singapour)
LIFE TECHNOLOGIES CORPORATION (USA)
Inventeur(s)
Wei, Han
Chan, Chee Wai
Liaw, Wui Khen
Dong, Shan Hua
Goh, See Chen
Yeo, Huei Steven
Sicat, Jr., Harmon Cosme
Ling, Mio Xiu Lu
Mead, Josh M.
Makinen, Mikko
Lim, Beng Heng
Boo, Kuan Moon
Bong, Justina Linkai
Ng, Chye Sin
Lee, Wee Liam
Lim, Li Yang
Lee, Way Xuang
Abrégé
An electroporation system including one or more of a pipette, a pipette tip, a pipette docking assembly, and a pulse generator. The pipette docking assembly includes a pipette station, a pipette station guard, and a reservoir (e.g., a buffer tube). A method for transfecting a cell with a payload including providing an electroporation system, providing the cell, providing the payload, introducing the cell and the payload into a pipette tip, and electroporating the cell within the pipette tip by operating the electroporation system.
In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.
The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligo-nucleotides can be used in a variety of nucleic acid sequencing methods.
A method for determining a genotype of a sample of a polyploid organism, may include: amplifying nucleic acid sequences at targeted locations in a sample genome by a panel targeting a plurality of SNP markers to generate sequence reads; mapping the sequence reads to a reference genome for the polyploid organism; detecting variants in the aligned sequence reads to produce detected variants, wherein the detected variants include detected SNP's corresponding to the SNP markers; determining a probability for each alternate allele dosage of a plurality of possible allele dosages for a corresponding detected SNP, wherein the number of possible allele dosages is equal to a ploidy of the SNP marker plus one; and selecting the alternate allele dosage with a maximum probability value to provide an estimated allele dosage corresponding to the SNP marker, wherein the estimated allele dosage is indicative of the genotype for the SNP marker.
Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.
A bioreactor pod includes a pod base; a dual mode bioreactor operable in static and dynamic modes and insertable into the pod base; one or more portable and arrangeable pod modules; a stand for mounting pod components; a display including a user interface for transmitting user inputs and receiving outputs associated with the dual mode bioreactor, pod modules; and a controller for controlling the bioreactor and pod modules.
Apparatus and instruments for clinical and medical diagnosis, namely, nucleic acid sequencers and synthesizers, genetic analyzers, and instruments for the preparation of nucleic acid samples
Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p.ex. séquençage par hybridation [SBH]
G01N 27/27 - Association de plusieurs systèmes ou cellules de mesure, chacun mesurant un paramètre différent, dans laquelle les résultats des mesures peuvent être, soit utilisès indépendamment, les systèmes ou les cellules étant physiquement associés, soit combin
42.
COMPOSITIONS, KITS AND METHODS FOR DETECTION OF VIRAL VARIANT SEQUENCES
Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
43.
COMPOSITIONS AND METHODS FOR CULTURING AND EXPANDING CELLS
Provided herein are, inter alia, compositions, systems, kits, and methods for culturing and expanding cells (such as T cells, diploid or non-diploid cells), as well as methods for treating disorders (e.g., with T cells), and methods for producing biological molecules and compositions (e.g., proteins, viruses, viral particles or fragments thereof, etc.), including vaccines.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
C12N 5/0783 - Cellules T; Cellules NK; Progéniteurs de cellules T ou NK
The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.
The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.
Systems and methods for measuring a foam layer in a fluid processing system are disclosed. The system includes a container having a chamber for containing a liquid and the foam layer. The system includes a first sensor positioned adjacent a top portion of the chamber. The first sensor is configured to measure a first distance between a top foam surface of the foam layer and the first sensor. The system includes a second sensor coupled to the container, the second sensor configured to measure a mass of the liquid. The system also includes a processor configured to perform the operations of calculating a thickness of the foam layer based on dimensions of the chamber of the container, the measured first distance between the top foam surface of the foam layer and the first sensor, and the mass of the liquid as measured by the second sensor.
G01F 17/00 - Procédés ou appareils pour la détermination de la capacité des récipients ou des cavités, ou du volume des corps solides
G01F 1/34 - Mesure du débit volumétrique ou du débit massique d'un fluide ou d'un matériau solide fluent, dans laquelle le fluide passe à travers un compteur par un écoulement continu en utilisant des effets mécaniques en mesurant la pression ou la différence de pression
G01S 13/08 - Systèmes pour mesurer la distance uniquement
G01S 17/88 - Systèmes lidar, spécialement adaptés pour des applications spécifiques
47.
COMPRESSION COLLAR EXPANDER ASSEMBLY AND METHOD OF USE
Various embodiments of a compression collar expander assembly for expanding a tubular compression collar are described. For example, the compression collar expander assembly can include a guide plate, a spindle plate, and a plurality of carriages movably disposed on a top surface of the guide plate. Each of the plurality of carriages can include a body, a guide projecting from the body, and a die set comprising a plurality of dies. Each die can include a base coupled to a corresponding one of the plurality of carriages and a prong upstanding from the base. In some embodiments, a tapered expander is configured to assist with expanding the plurality of dies.
Energy transfer dye pairs including a donor dye covalently attached to an acceptor dye through a linker, uses of the energy transfer dye pairs, for example, in conjugates of an energy transfer dye pair covalently attached to a quencher and an analyte (e.g., an oligonucleotide), for biological applications including, for example, amplification assays such as quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR).
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p.ex. transfert d’énergie de résonance
C09B 62/343 - Colorants réactifs, c. à d. colorants formant des liaisons de covalence avec les substrats ou se polymérisant sur eux-mêmes le groupe réactif est directement lié à un hétérocycle à un cycle à cinq chaînons
Disclosed is an integrated biocontainment cell sorter that isolates portions of the cell sorter that can create contamination. Two containment systems are utilized. A main cabinet containment system contains input samples. An aerosol management containment area includes a nozzle chamber with a nozzle and a sort chamber with sort plates and collection media that collect a droplet stream from the nozzle. The main cabinet is maintained at a first low pressure and clean air is recirculated under a positive pressure. The aerosol management containment area is kept at a second low pressure, which is lower than the first pressure, so that contamination does not leak from the aerosol management containment area into the main cabinet containment area. A sliding sash window is located over an access opening in the main cabinet and can be moved to access different portions of the main cabinet without changing the substantially constant first low pressure in the main cabinet.
This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.
G01N 33/58 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des substances marquées
A61K 47/10 - Alcools; Phénols; Leurs sels, p.ex. glycérol; Polyéthylène glycols [PEG]; Poloxamères; Alkyléthers de PEG/POE
A61K 47/18 - Amines; Amides; Urées; Composés d’ammonium quaternaire; Acides aminés; Oligopeptides ayant jusqu’à cinq acides aminés
G01N 33/533 - Production de composés immunochimiques marqués avec un marqueur fluorescent
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
53.
Compression Collars for Coupling a Tube to a Tube Fitting
A coupling assembly includes: a tubular compression collar having a tubular body made of a resiliently flexible polymeric material and having an interior surface and an opposing exterior surface; an end of a tube disposed within a throughway of the compression collar; and a tube fitting disposed within the passageway of the tube. The tube fitting includes: a tubular stem; a flange radially outwardly projecting from an exterior surface of the stem; and an annular barb encircling and radially outwardly projecting from the exterior surface of the stem, the annular barb including a frustoconical outside face that extends along and outwardly slopes away from the stem as the outside face extends toward the flange. The compression collar radially inwardly compresses the tube against the annular barb of the tube fitting so that a liquid tight seal is formed between the tube and the tube fitting.
F16L 33/207 - Segments, manchons ou autres organes d'une seule pièce enserrant la manche ou dilatés à l'intérieur de la manche au moyen d'outils; Agencements utilisant de tels organes avec uniquement un manchon contracté sur la manche
F16L 33/22 - Dispositions d'assemblage des manches avec des organes rigides; Raccords rigides pour manches, p.ex. éléments unitaires s'engageant à la fois dans deux manches avec moyens non mentionnés dans les groupes précédents pour saisir la manche entre l'extérieur et l'intérieur
B29C 65/00 - Assemblage d'éléments préformés; Appareils à cet effet
B29C 65/68 - Assemblage d'éléments préformés; Appareils à cet effet par libération de contraintes internes, p.ex. par retrait d'un des éléments à assembler en utilisant un élément auxiliaire rétractable
B29C 65/56 - Assemblage d'éléments préformés; Appareils à cet effet en utilisant des moyens mécaniques
A61M 39/12 - Raccords ou accouplements pour tubes pour raccorder un tube flexible à un dispositif de fixation rigide
Provided herein are ionizable lipids, compositions comprising the ionizable lipids, and methods of making and using the same. The ionizable lipids provided herein can be formulated in lipid compositions for the delivery of macromolecules, such as nucleic acids, in vitro, ex vivo, or in vivo.
C07D 265/30 - Oxazines-1, 4; Oxazines-1, 4 hydrogénées non condensées avec d'autres cycles
C07D 279/12 - Thiazines-1, 4; Thiazines-1, 4 hydrogénées non condensés avec d'autres cycles
C07D 295/145 - Composés hétérocycliques contenant des cycles polyméthylène imine d'au moins cinq chaînons, des cycles aza-3 bicyclo [3.2.2] nonane, piperazine, morpholine ou thiomorpholine, ne comportant que des atomes d'hydrogène liés directement aux atomes de car avec des radicaux hydrocarbonés substitués liés aux atomes d'azote du cycle substitués par des atomes de carbone comportant trois liaisons à des hétéro-atomes avec au plus une liaison à un halogène, p.ex. radicaux ester ou nitrile avec les atomes d'azote du cycle et les atomes de carbone comportant trois liaisons à des hétéro-atomes liés à la même chaîne carbonée, qui n'est pas interrompue par des carbocycles
A61K 48/00 - Préparations médicinales contenant du matériel génétique qui est introduit dans des cellules du corps vivant pour traiter des maladies génétiques; Thérapie génique
55.
COMPOSITIONS, KITS, AND METHODS FOR DETECTION OF VARIANT STRAINS OF AFRICAN SWINE FEVER VIRUS
Disclosed are compositions, methods, systems, and kits for the detection of African swine fever virus (ASFV) in a test sample, and in particular for distinguishing between wild/reference type ASFV and mutant/variant strains of ASFV. A variant ASFV assay includes a first set of primers and a first probe that correspond to a first ASFV target, and a second set of primers and a second probe that correspond to a second ASFV target. The first and second probes are differentially labelled. The first ASFV target is a MGF360 gene and the second ASFV target is the CD2v gene. Absence of these targets, in conjunction with a positive determination for another generic ASFV target such as the p72 gene, is indicative of a vaccine-associated variant strain of ASFV.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Provided herein, inter alia, are chemically defined components and compositions that substitute or partially substitute for albumin in cell culture media. The components and compositions may support cell cultures, protect cells, or enhance viability of cultured cells. Further provided are chemically defined culture media supplements for use in cell culture media containing albumin. The chemically defined culture media supplements may rescue cells from albumin-induced toxicity.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
A method for coupling a tube to a tube fitting includes radially outwardly expanding a tubular compression collar from a constricted state to an expanded state, the compression collar having a throughway extending there through and being made of a resiliently flexible material. An end of the tube is inserted within the throughway of the expanded compression collar, the tube bounding a passage. A tube fitting is inserted within the passage of the tube. The compression collar is allowed to resiliently rebound back towards the constricted state so that the compression collar pushes the tube against the tube fitting.
F16L 33/207 - Segments, manchons ou autres organes d'une seule pièce enserrant la manche ou dilatés à l'intérieur de la manche au moyen d'outils; Agencements utilisant de tels organes avec uniquement un manchon contracté sur la manche
F16L 33/22 - Dispositions d'assemblage des manches avec des organes rigides; Raccords rigides pour manches, p.ex. éléments unitaires s'engageant à la fois dans deux manches avec moyens non mentionnés dans les groupes précédents pour saisir la manche entre l'extérieur et l'intérieur
B29C 65/00 - Assemblage d'éléments préformés; Appareils à cet effet
B29C 65/68 - Assemblage d'éléments préformés; Appareils à cet effet par libération de contraintes internes, p.ex. par retrait d'un des éléments à assembler en utilisant un élément auxiliaire rétractable
B29C 65/56 - Assemblage d'éléments préformés; Appareils à cet effet en utilisant des moyens mécaniques
A61M 39/12 - Raccords ou accouplements pour tubes pour raccorder un tube flexible à un dispositif de fixation rigide
59.
CYCLE THRESHOLDS IN MACHINE LEARNING FOR FORECASTING INFECTION COUNTS
Methods for forecasting case counts for a future date in one or more geographic areas of persons infected by a disease is disclosed. The presence of the disease in a biological sample is testable by a polymerase chain reaction (PCR) test. A load of one or more pathogens associated with the disease correlates with a PCR cycle which indicates presence of the one or more pathogens, and is referred to as a threshold cycle (Ct). Data relevant to forecasting the case counts including Ct data and other data is received. The Ct data comprises Ct values from PCR tests of biological samples from persons within the one or more geographic areas. Arrays of feature data for processing by a trained machine learning model are generated, comprising Ct features and other features obtained from the data. A forecasted number of infected persons are generated by processing the arrays using machine learning.
G16H 50/50 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicales; TIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour la simulation ou la modélisation des troubles médicaux
G16H 50/80 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicales; TIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour la détection, le suivi ou la modélisation d’épidémies ou des pandémies, p.ex. de la grippe
60.
METHODS AND SYSTEMS TO DETECT LARGE REARRANGEMENTS IN BRCA1/2
A method for detecting large rearrangements in BRCA1 and BRCA2 genes includes amplifying a nucleic acid sample in the presence of a primer pool to produce amplicons, where the primer pool includes target specific primers targeting regions of exons of the BRCA1 and BRCA2 genes. The method further includes sequencing the amplicons to generate a plurality of reads, mapping the reads to a reference sequence, determining a number of reads per amplicon for the amplicons associated with the exons of the BRCA and the BRCA2 genes, determining exon copy numbers for the exons of the BRCA1 and BRCA2 genes based on the number of reads per amplicon, detecting an exon deletion or duplication based on the exon copy numbers, and detecting a whole gene deletion of the BRCA1 or BRCA2 gene based on the number of reads per amplicon associated with the exons of the BRCA1 and BRCA2 genes.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 40/00 - TIC spécialement adaptées aux biostatistiques; TIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p.ex. extraction de connaissances ou détection de motifs
G16B 20/10 - Ploïdie ou détection du nombre de copies
A method of modeling a background signal when sequencing a polynucleotide strand using sequencing-by-synthesis includes: flowing a series of nucleotide flows onto a reactor array having multiple reaction confinement regions, one or more copies of the polynucleotide strand being located in a loaded reaction confinement region of the reactor array, the loaded reaction confinement region being located in a vicinity of one or more neighboring reaction confinement regions that may or may not be loaded; receiving output signals from the reactor array; and modeling a background signal for the loaded reaction confinement region using the received output signals and a model adapted to account at least for an exchange of ions between the one or more neighboring reaction confinement regions and a headspace adjacent the loaded reaction confinement region and the one or more neighboring reaction confinement regions.
G16B 5/00 - TIC spécialement adaptées à la modélisation ou aux simulations dans la biologie des systèmes, p. ex. réseaux de régulation génétique, réseaux d’interaction entre protéines ou réseaux métaboliques
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p.ex. séquençage par hybridation [SBH]
G16B 25/00 - TIC spécialement adaptées à l’hybridation; TIC spécialement adaptées à l’expression de gènes ou de protéines
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
63.
TEMPERATURE INSULATED PACKAGING SYSTEMS AND RELATED METHODS
A temperature insulated packaging system includes a container having interior surface bounding an interior volume and a liner disposed within the interior volume and at least partially bounding a compartment configured to receive an item for temperature insulated shipping. The liner includes a first sleeve made of cellulose material and at least partially bounds a channel. The first sleeve has an outside wall disposed adjacent to the container and an opposing inside wall disposed adjacent to the compartment configured to receive the item for shipping, the channel being disposed between the inside wall and the outside wall. At least one insulation sheet is disposed within the channel of the first sleeve, the at least one insulation sheet being made of a cellulose material and having a plurality of recesses formed thereon.
B65D 81/38 - Réceptacles, éléments d'emballage ou paquets pour contenus présentant des problèmes particuliers de stockage ou de transport ou adaptés pour servir à d'autres fins que l'emballage après avoir été vidés de leur contenu avec isolation thermique
B65D 5/56 - Revêtements ou recouvrements intérieurs
The present disclosure generally relates to compositions and methods for the synthesis of nucleic acid molecules with low error rates. Provided, as examples, are compositions and methods for high throughput synthesis and assembly of nucleic acid molecules, in many instances, with high sequence fidelity. In many instances, thermostable mismatch recognition proteins (e.g., thermostable mismatch binding protein, thermostable mismatch endonucleases) will be present in compositions and use methods provided.
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p.ex. acides nucléiques
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
Methods for forecasting case counts for a future date in one or more geographic areas of persons infected by a disease is disclosed. The presence of the disease in a biological sample is testable by a polymerase chain reaction (PCR) test. A load of one or more pathogens associated with the disease correlates with a PCR cycle which indicates presence of the one or more pathogens, and is referred to as a threshold cycle (Ct). Data relevant to forecasting the case counts including Ct data and other data is received. The Ct data comprises Ct values from PCR tests of biological samples from persons within the one or more geographic areas. Arrays of feature data for processing by a trained machine learning model are generated, comprising Ct features and other features obtained from the data. A forecasted number of infected persons are generated by processing the arrays using machine learning.
G16H 50/80 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicales; TIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour la détection, le suivi ou la modélisation d’épidémies ou des pandémies, p.ex. de la grippe
G16H 50/20 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicales; TIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le diagnostic assisté par ordinateur, p.ex. basé sur des systèmes experts médicaux
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
66.
MULTI-COLUMN CHROMATOGRAPHY SYSTEMS WITH ROTATABLE VALVE ASSEMBLIES
A chromatography system includes a first chromatography column and a panel having a top face and an opposing bottom face, a first cavity being formed on the panel so as to pass through the top face and be encircled by an inner surface. The panel bounds an inlet fluid channel having an end terminating at an inlet opening formed on the inner surface encircling the first cavity so that the inlet fluid channel communicates with the first cavity. The panel also bounds a plurality of first outlet fluid channels each having an end terminating at an outlet opening formed on the inner surface encircling the first cavity so that each of the plurality of first outlet fluid channels communicate with the first cavity, a first one of the plurality of first outlet fluid channels being in fluid communication with the first chromatography column. A first valve is rotatably disposed within the first cavity.
B01D 15/18 - Adsorption sélective, p.ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives aux différents types d'écoulement
B01D 15/14 - Adsorption sélective, p.ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à l'introduction de l'alimentation dans l'appareil
Described herein are various methods and embodiments of a fluid processing system and foam breaker devices for at least reducing foam formation in a container of the fluid processing system. In some embodiments, the foam breaker device includes a mounting hub extending along a longitudinal axis of the foam breaker device. The mounting hub can include a coupling feature for coupling the foam breaker device to a drive shaft of the fluid processing system. The foam breaker device can also include a wall structure extending circumferentially from the mounting hub at an angle relative to the longitudinal axis of the foam breaker device. The wall structure can cause a reduction in the foam volume as a result of at least a part of the wall structure contacting the foam volume.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
B01D 45/00 - Séparation de particules dispersées dans des gaz ou des vapeurs par gravité, inertie ou force centrifuge
B01F 27/90 - Mélangeurs à agitateurs tournant dans des récipients fixes; Pétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des palettes ou des bras
Particles This invention relates to magnetic clusters comprising nanocrystals of iron oxide. The magnetic clusters comprise an aromatic carboxylic acid and/or the magnetic clusters are monodisperse. The magnetic clusters may be useful in biological assays and other applications. The invention also relates to processes for preparing such magnetic clusters and methods of using the magnetic clusters, as well kits comprising the magnetic clusters.
H01F 1/00 - Aimants ou corps magnétiques, caractérisés par les matériaux magnétiques appropriés; Emploi de matériaux spécifiés pour leurs propriétés magnétiques
69.
SYSTEMS AND METHODS FOR IDENTIFYING SEQUENCE VARIATION
Systems and method for determining variants can receive mapped reads, and call variants. In embodiments, flow space information for the reads can be aligned to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be grouped and a score can be calculated for the variant. Based on the scores, a list of probable variants can be provided. In various embodiments, low frequency variants can be identified where multiple potential variants are present at a position.
09 - Appareils et instruments scientifiques et électriques
Produits et services
(Based on Use in Commerce) Chromatography columns for laboratory use; chromatography columns for the development and manufacturing of biologics; (Based on Intent To Use) ; Plastic laboratory consumables, namely 96-well plates
C08B 37/00 - Préparation des polysaccharides non prévus dans les groupes ; Leurs dérivés
G01N 33/532 - Production de composés immunochimiques marqués
G01N 33/543 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
B01D 15/38 - Adsorption sélective, p.ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p.ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
G01N 33/53 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet
G01N 33/554 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques le support étant une cellule ou un fragment de cellule biologique, p.ex. cellules de bactéries, de levure
G01N 33/569 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet pour micro-organismes, p.ex. protozoaires, bactéries, virus
C07K 16/00 - Immunoglobulines, p.ex. anticorps monoclonaux ou polyclonaux
G01N 33/58 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des substances marquées
C07D 403/12 - Composés hétérocycliques contenant plusieurs hétérocycles, comportant des atomes d'azote comme uniques hétéro-atomes du cycle, non prévus par le groupe contenant deux hétérocycles liés par une chaîne contenant des hétéro-atomes comme chaînons
C12N 5/0783 - Cellules T; Cellules NK; Progéniteurs de cellules T ou NK
G01N 33/533 - Production de composés immunochimiques marqués avec un marqueur fluorescent
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.
A61J 1/14 - Récipients spécialement adaptés à des fins médicales ou pharmaceutiques - Détails; Accessoires à cet effet
B65B 3/00 - Emballage de matériaux plastiques, de semi-liquides, de liquides ou de liquides et solides mélangés, dans des réceptacles ou récipients individuels, p.ex. dans des sacs ou sachets, boîtes, cartons, bidons ou pots
B65B 31/04 - Mise sous vide, sous pression ou sous gaz spécial, des réceptacles ou emballages pleins, au moyen d'ajutages par lesquels on envoie ou on retire de l'air ou d'autres gaz, p.ex. un gaz inerte
B65D 8/00 - Réceptacles de section transversale courbe, dont le corps est formé par jonction ou liaison de plusieurs composants rigides, ou sensiblement rigides, constitués en totalité ou principalement en métal, en matière plastique, en bois ou en un matériau d
C12N 9/00 - Enzymes, p.ex. ligases (6.); Proenzymes; Compositions les contenant; Procédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes
Provided herein, are compositions, methods and kits for inducing an immune response in a subject. In aspects, a lipid composition is described, which includes at least one ionizable lipid comprising a charge (N), at least one peptide, and a nucleic acid molecule comprising a charge (P). In aspects, methods are provided for delivery of a payload to an immune cell using a lipid composition comprising at least one ionizable lipid, at least one endosomal release peptide, and a payload.
A61K 47/69 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p.ex. les supports ou les additifs inertes; Agents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p.ex. conjugués polymère-médicament le conjugué étant caractérisé par sa forme physique ou sa forme galénique, p.ex. émulsion, particule, complexe d’inclusion, stent ou kit
A61K 47/58 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p.ex. les supports ou les additifs inertes; Agents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p.ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique macromoléculaire, p.ex. une molécule oligomérique, polymérique ou dendrimérique obtenu par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone, p.ex. poly[méth]acrylate, polyacrylamide, polystyrène, polyvinylpyrrolidone, alcool polyvinylique ou résine d’acide sulfonique de polystyrène
An in vitro method, composition and kit for determining the presence or absence of adenovirus, metapneumovirus, rhinovirus/enterovirus, and parainfluenza in a sample, including providing a reaction mixture containing the sample and at least one primer pair set. The primer pair set includes at least one primer pair A that specifically amplifies a portion of adenovirus genome; at least one primer pair B that specifically amplifies a portion of metapneumovirus genome; at least one primer pair C that specifically amplifies a portion of rhinovirus/enterovirus genome; and at least one primer pair D that specifically amplifies a portion of parainfluenza genome. The reaction mixture is subjected to reaction conditions suitable to amplify targeted nucleic acids, thereby generating at least one amplicon; wherein the presence or absence of at least one amplicon in the sample indicates the presence or absence of adenovirus, metapneumovirus, rhinovirus/enterovirus, and/or parainfluenza in the sample.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
Methods for assessing genomic instability (GI) may include selectively amplifying nucleic acid sequences at targeted locations in a tumor sample genome by a targeted panel with a low sample input to generate a plurality of nucleic acid sequence reads; dividing the genome into segments having homogeneous copy numbers using log odds of heterozygous SNPs and CNV log ratios determined for the nucleic acid sequence reads; applying a threshold count to squared allelic log odds of each segment in autosomes of the genome to identify unbalanced copy number (UCN) segments; adding numbers of bases in the UCN segments to produce a sum of UCN bases; and dividing the sum of UCN bases by the total number of bases in all the segments identified in the autosomes of the sample genome to produce a ratio indicative of genomic instability. The ratio may be expressed as a percent to give a GI score.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
G16B 20/10 - Ploïdie ou détection du nombre de copies
77.
COMPOSITIONS, KITS, AND METHODS FOR DETECTING NUCLEIC ACIDS USING INTRA-CHANNEL MULTIPLEXING
Disclosed are compositions, kits, and methods that enable intra-channel multiplexing by enabling determination of separate detectable signals, each associated with a different assay target, within the same detection channel. The multiple detectable signals can be separately resolved and independently analyzed to enable detection and/or quantification of each respective target. Enabling multiple targets to be assayed within the same detection channel increases the plexy of multiplex assays without relying on additional dyes and concomitant issues of increased spectral overlap.
Provided herein, are compositions, methods and kits for inducing an immune response in a subject. In aspects, a lipid composition is described, which includes at least one ionizable lipid comprising a charge (N), at least one peptide, and a nucleic acid molecule comprising a charge (P). In aspects, methods are provided for delivery of a payload to an immune cell using a lipid composition comprising at least one ionizable lipid, at least one endosomal release peptide, and a payload.
A61K 47/64 - Conjugués médicament-peptide, médicament-protéine ou médicament-acide polyaminé, c. à d. l’agent de modification étant un peptide, une protéine ou un acide polyaminé lié par covalence ou complexé à un agent thérapeutiquement actif
A61K 47/54 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p.ex. les supports ou les additifs inertes; Agents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p.ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique
C07K 7/08 - Peptides linéaires ne contenant que des liaisons peptidiques normales ayant de 12 à 20 amino-acides
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p.ex. co-transformation
Systems, methods, software and computer-usable media for annotating biomolecule-related data are disclosed. In certain exemplified embodiments, the biomolecules can be nucleic acids and the data can be sequence-related data. In various embodiments, systems can include one or more public or private biological attributes (e.g., annotation information databases, data storage devices and systems, etc.) sources, one or more genomic features data sources (e.g., genomic variant tools, genomic variant databases, genomic variant data storage devices and systems, etc.), a computing device (e.g., workstation, server, personal computer, mobile device, etc.) hosting an annotations module and/or a reporting module, and a client terminal.
G16B 40/00 - TIC spécialement adaptées aux biostatistiques; TIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p.ex. extraction de connaissances ou détection de motifs
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
Methods for assessing genomic instability (GI) may include selectively amplifying nucleic acid sequences at targeted locations in a tumor sample genome by a targeted panel with a low sample input to generate a plurality of nucleic acid sequence reads; dividing the genome into segments having homogeneous copy numbers using log odds of heterozygous SNPs and CNV log ratios determined for the nucleic acid sequence reads; applying a threshold count to squared allelic log odds of each segment in autosomes of the genome to identify unbalanced copy number (UCN) segments; adding numbers of bases in the UCN segments to produce a sum of UCN bases; and dividing the sum of UCN bases by the total number of bases in all the segments identified in the autosomes of the sample genome to produce a ratio indicative of genomic instability. The ratio may be expressed as a percent to give a GI score.
Systems and methods that enable analyte detection in a multiplexed amplification process can include obtaining, at multiple time points during the amplification process, composite emission signal data associated with a composite emission signal from at least a first probe type comprising a first label configured to generate a first emission signal and a second probe type comprising a second label configured to generate a second emission signal which has spectrally similar characteristics as said first emission signal, the first probe type and the second probe type differing in thermal and/or temporal properties; and determining, based at least partially on the composite emission signal data, emission signal data associated with a emission signal from a given probe type of the first probe type or the second probe type during the amplification process.
The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.
LIFE TECHNOLOGIES HOLDINGS PTE LIMITED (Singapour)
LIFE TECHNOLOGIES CORPORATION (USA)
Inventeur(s)
Chen, Ming Song
Chong, Chee Woei
Loh, Wuh Ken
Foo, Wern Yuh
Boo, Kuan Moon
Leclerc, Norbert
Uhlendorf, Kristina
Koellner, Reiner
Fuchs, Torsten
Breitfelder, Stefan
Abrégé
A biological analysis system can include an excitation module and an emission module. The excitation module can include a collimator element for receiving excitation light from the excitation light source and transmitting collimated excitation light in a first direction, and a plurality of excitation mirrors arrayed along the excitation light path, each excitation mirror disposed at an acute angle relative to the first direction and configured to reflect collimated excitation light along a second direction. The emission module can be positioned to receive excitation light transmitted along the second direction and can include a sample block comprising a plurality of sample receptacles positioned to receive a beam of collimated excitation light, and a plurality of photodetectors configured to receive emission light transmitted from a respective sample receptacle in a direction transverse to the second direction of the excitation light path.
The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of B cell receptor heavy and light chains in a single assay, with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
87.
Apparatuses, Systems And Methods For Imaging Flow Cytometry
The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.
A method for processing a fluid includes removably securing a retention member to a vessel that bounds a chamber; inserting a collapsible bag within the chamber of the vessel; securing the bag to the retention member so that the bag is supported within the chamber of the vessel; and dispensing a fluid into a compartment of the collapsible bag supported within the chamber of the vessel. The fluid can be mixed within bag while the bag is disposed within the vessel.
B01F 27/88 - Mélangeurs à agitateurs tournant dans des récipients fixes; Pétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec une unité récipient-agitateur séparée qui est adaptée pour être couplée à un mécanisme d'entraînement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p.ex. avec agitateur à turbine
B01F 23/233 - Mélange de gaz avec des liquides en introduisant des gaz dans des milieux liquides, p.ex. pour produire des liquides aérés en utilisant des agitateurs entraînés munis d’éléments d'agitation complètement immergés
B01F 23/231 - Mélange de gaz avec des liquides en introduisant des gaz dans des milieux liquides, p.ex. pour produire des liquides aérés par barbotage
B01F 27/91 - Mélangeurs à agitateurs tournant dans des récipients fixes; Pétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des hélices
B01F 27/213 - Mélangeurs à agitateurs tournant dans des récipients fixes; Pétrins caractérisés par leurs arbres de rotation caractérisés par la liaison avec l'entraînement
B01F 27/2121 - Mélangeurs à agitateurs tournant dans des récipients fixes; Pétrins caractérisés par leurs arbres de rotation composés de parties interconnectées
B01F 33/00 - Autres mélangeurs; Installations pour effectuer des mélanges; Combinaisons de mélangeurs
B01F 33/501 - Dispositifs de mélange mobiles, c. à. d. facilement déplaçables d'un endroit à l'autre, p.ex. portables pendant l'utilisation
B01F 35/41 - Montage ou support des arbres d'agitation ou des unités d'agitation sur les récipients
B01F 35/45 - Fermetures ou portes spécialement adaptées aux récipients de mélange; Mécanismes de fonctionnement à cet effet
B01F 35/513 - Récipients souples, p.ex. sacs supportés par des conteneurs rigides
B65B 1/02 - Machines caractérisées par l'incorporation de moyens pour fabriquer les réceptacles ou récipients
A skid (700) for use in separating biocomponents includes a housing (701) bounding a compartment (708), the compartment being partially bounding by a mounting platform (709); and a loading assembly (800) secured to the housing so as to communicate with the compartment. The loading assembly (800) includes an alignment plate (808) having a top surface with cavity (814) recessed therein, the cavity communicating with the compartment; a drive rotor (15) rotatably disposed below the alignment plate and at least partially encircling the cavity, the drive rotor including one or more magnets; a motor (169) coupled to the drive rotor for selectively rotating the drive rotor about the cavity; and a mount at least partially encircling the drive rotor and communicating with the compartment, the mount including a mounting plate having one or more mounting elements upstanding therefrom, the mount being movable between a raised position wherein the mounting plate is aligned with the alignment plate and a second lowered position wherein the mounting plate is disposed at an elevation lower than the alignment plate.
A method for labeling sequence reads includes retrieving a sequence read having an associated flow measurement and an associated flow order; matching a sequence selected from a plurality of sequences with the sequence read, the sequence having a position within the sequence that has more than one acceptable variants; determining which variant of the more than one acceptable variants matches the sequence; generating a predicted flow measurement based on the matched sequence, the variant, and a flow order; and labeling the sequence read and associated flow measurement with the predicted flow measurement.
A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.
A system facilitating parallel laboratory operations which includes a plurality of labware components, and a plurality of processing heads configured to interact with the plurality of labware components is described. The system further includes a first set of actuators coupled to the plurality of processing heads and configured to actuate the plurality of processing heads along a first directional axis. The system further includes a second set of actuators configured to translate the plurality of labware components along at least a second directional axis and a third directional axis. The second set of actuators may include one or more magnetic levitation systems configured to cause movement of the plurality of labware components along the second directional axis and the third directional axis.
A computer-implemented method for classifying alignments of paired nucleic acid sequence reads is disclosed. A plurality of paired nucleic acid sequence reads is received, wherein each read is comprised of a first tag and a second tag separated by an insert region. Potential alignments for the first and second tags of each read to a reference sequence is determined, wherein the potential alignments satisfies a minimum threshold mismatch constraint. Potential paired alignments of the first and second tags of each read are identified, wherein a distance between the first and second tags of each potential paired alignment is within an estimated insert size range. An alignment score is calculated for each potential paired alignment based on a distance between the first and second tags and a total number of mismatches for each tag.
G16B 30/10 - Alignement de séquence; Recherche d’homologie
G16B 40/00 - TIC spécialement adaptées aux biostatistiques; TIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p.ex. extraction de connaissances ou détection de motifs
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
The present set of embodiments relate to a system, method, and apparatus for culturing cells within a cell culture vessel having a mixing element. The cell culture system includes a flexible portion and a mixing element disposed therein. The mixing element includes a suspended foldable portion. The system is configured to reduce shear stress on cells without compromising mixing efficiency. This reduction is accomplished by using a mixing element having a large surface area allowing for reduced rotational speeds. The system is collapsible for ease of transport and disposal. The flexible portion collapses and the foldable portion folds to minimize the volume of the system while not in operation.
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p.ex. avec agitateur à turbine
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
B01F 27/054 - Agitateurs déformables, p.ex. déformés par une force centrifuge appliquée en cours de fonctionnement
B01F 27/072 - Agitateurs caractérisés par leur montage sur l’arbre caractérisés par la disposition des agitateurs par rapport à l'axe de rotation
B01F 27/2124 - Arbres à longueur réglable, p.ex. arbres télescopiques
B01F 27/1125 - Agitateurs caractérisés par la configuration des agitateurs avec des bras, des pales ou des lames avec des pales ou des lames s'étendant parallèlement ou obliquement par rapport à l'axe de l'agitateur
B01F 35/513 - Récipients souples, p.ex. sacs supportés par des conteneurs rigides
96.
COMPOSITIONS AND METHODS FOR NUCLEIC ACID ISOLATION
A method of separating RNA from a sample, comprising providing: a sample comprising RNA, a binding solution comprising an oligoethylene glycol and a salt, and a solid support having a hydrophilic surface. The method further comprises contacting the sample with the binding solution and solid support, under conditions that allow binding of the RNA in the sample to the surface of the solid support, thereby providing a solid support with bound RNA in contact with residual solution; and separating the solid support with bound RNA from the residual solution. During the binding of the RNA to the surface of the solid support, oligoethylene glycol is present at a concentration of at least about 35% v/v and the salt is present at a concentration of between about 1 M and about 2 M. Methods for producing purified RNA are also provided, comprising in vitro transcription to produce an RNA molecule with a first magnetic bead and purification with a second magnetic bead. Also provided are kits and uses.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Cell culture media, mammalian cells, stem cell reagents,
cryopreservation reagents, cell culture reagents, cell
culture assays, transfection reagents, all the
aforementioned for research use only.
A system and method of selecting genes for a gene panel, includes retrieving gene-disease associations of genes associated with diseases at a given level in the disease hierarchy from a disease association database. The disease association database stores disease information, gene information, phenotype information, associations between diseases in the disease hierarchy, gene-disease associations and strength parameters related to the gene-disease associations. For each gene associated with the diseases at the given level, the strength parameters are weighted and combined to determine a rank score for the each gene. The genes are ranked based on the rank scores to provide ranked gene information. The ranked gene information is linked with diseases at the higher levels of the disease hierarchy based on hierarchical relationships. The ranked gene information for gene-disease associations can be used to select genes for a gene panel design.
Systems and methods for automated laser microdissection are disclosed including automatic slide detection, position detection of cutting and capture lasers, focus optimization for cutting and capture lasers, energy and duration optimization for cutting and capture lasers, inspection and second phase capture and/or ablation in a quality control station and tracking information for linking substrate carrier or output microdissected regions with input sample or slide.
