The present invention resides in a multichannel head of a pipetting device for aspirating and dispensing liquid via a plurality of pipetting tips. The multichannel head comprises a connector block (110) having an array of m*n connectors (120), whereby each connector comprises an internal passageway (125) that is fluidically connectable to a pipetting tip, and further includes a plunger block having a corresponding array of m*n channels. Each channel accommodates a plunger and has an opening that is fluidically connected to the internal passageway (125) of a corresponding connector. The multichannel head further comprises a sealing mat (150) arranged between the connector block (110) and the plunger block, wherein the sealing mat comprises a corresponding array of m*n annular sealing elements for providing an airtight seal of the fluidic connection between the internal passageway of each connector and each opening of the corresponding plunger channel. The annular sealing elements are interconnected by a lattice of linkages that extend in lateral direction and in longitudinal direction, such that a cut-out region is formed between two opposing lateral linkages and two opposing longitudinal linkages. The sealing mat (150) is arranged on a receiving surface (116), which is provided with protrusions (117) that are shaped to pass through the cut-out regions of the sealing mat.
A cooling module (106' ) for receiving a plurality of containers (116), said cooling module (106' ) comprising a housing (122) including at least one opening (124), a cooling block arranged into the housing (122), at least one compartment for receiving the containers (116) via the opening (124), and at least one cover ( 108, 108 ', 108 ' ' ) for covering the opening (124) against the exterior.
B01L 7/00 - Appareils de chauffage ou de refroidissement; Dispositifs d'isolation thermique
B01L 7/04 - Dispositifs d'isolation thermique, p.ex. manchons pour ballons
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
B01L 9/06 - Supports de tubes à essai; Porte-tubes à essai
3.
3D-PRINTED MASK FOR LYSING AT A LOCATION ON A TISSUE SECTION
Some embodiments are directed to a method for detaching tissue from a tissue section on a tissue slide. The method may include applying a 3D printed mask on top of the tissue section, the mask comprising barriers that define a cavity surrounding an area of interest on the tissue section, the cavity being open at a side facing the area of interest on the tissue section, dispensing a detaching liquid to the cavity, and allowing the detaching liquid to detach tissue at the area of interest, and aspirating the detaching liquid with the detached tissue from the cavity, and forwarding the liquid for further processing.
A dark-field imaging system comprising a platform for holding biologic tissue or cells, constituting a plane forming two half-spaces. The dark-field imaging system further comprises a light source, which generates an incident light targeting the biologic tissue or cells, and a camera to detect diffuse reflection off the biologic tissue or cells. The light source is placed at a first angle (a) relative to the plane, and the camera is placed at a second angle (β) relative to the plane wherein the light source, the camera, and the biologic tissue or cells are arranged in the same one of the two half-spaces formed by the plane. The incident light that is not reflected by the biologic tissue or cells reaches the platform, which comprises a specularly reflecting material that reflects the incident light at least in part by specular reflection. Use of such a dark-field imaging system in an application for interacting with areas of interest of biological samples, where the dark-field imaging system takes high-contrast images of biologic tissue or cells in an automated laboratory system, and an automated laboratory system comprising such a dark-field imaging system.
A method for minimizing the dead volume (Vd) in a vial (10) presenting a liquid (L) for aspiration is provided. The method comprises, after determining that the liquid volume (Vi) indicated to be available in the vial (10) is not sufficient for a further aspiration step, moving downward in direction towards the bottom of the vial (10) by means of a pipetting arm (20) presenting a pipetting tip (21) and along the z-axis of the pipetting arm (20) until the pipetting arm (20) comes to a stop in a first stop position (Slz). Then, the actual available liquid volume (Va) is calculated based i.a. on said first stop position (Slz). In case the calculated actual available liquid volume (Va) is sufficient, a further aspiration step can be executed. Furthermore, a liquid handling system for minimizing dead volume (Vd), a computer-implemented method for controlling a pipetting arm (20) of such a liquid handling system, and the use of a hysteresis determination method for minimizing dead volume (Vd) are provided.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
A rotary valve (10) comprises a stator member (36) with a stator face (54), the stator member (36) having at least two stator channels (46, 48) for conducting a fluid and opening into the stator face (54); a rotor member (24) with a rotor face (52) facing and in contact with the stator face (54), the rotor member (24) having a rotor channel (50) in the rotor face (52), wherein the rotor member (24) is rotatable with respect to the stator member (36) about a rotation axis (A), such that in a conducting position, the rotor channel (50) interconnects the at least two stator channels (46, 48) to be in fluid communication; and a rotary encoder assembly (33) adapted for determining a rotary position of the rotor member (24), the rotary encoder assembly (33) comprising an encoder member (34) and an encoder module (35), which is adapted to sense a rotary position of the encoder member (34). The encoder member (34) is rigidly connected to the rotor member (24) and surrounds the rotor member (24).
F16K 11/072 - Soupapes ou clapets à voies multiples, p.ex. clapets mélangeurs; Raccords de tuyauteries comportant de tels clapets ou soupapes; Aménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont toutes les faces d'obturation se déplacent comme un tout comportant uniquement des tiroirs à éléments de fermeture articulés à pivot
F16K 31/04 - Moyens de fonctionnement; Dispositifs de retour à la position de repos magnétiques utilisant un moteur
F16K 37/00 - Moyens particuliers portés par ou sur les soupapes ou autres dispositifs d'obturation pour repérer ou enregistrer leur fonctionnement ou pour permettre de donner l'alarme
A rotary valve (10) comprises a stator member (36) with a planar stator face, the stator member (36) having a plurality of stator channels (46, 48) for conducting a fluid; and a rotor member (24) with a planar rotor face (52) facing and in contact with the stator face, the rotor member (24) having a rotor channel (50); wherein the rotor member (24) is rotatable with respect to the stator member (36) about a rotation axis (A), such that in a conducting position, the rotor channel (50) interconnects two of the stator channels (46, 48) and the two stator channels (46, 48) are in fluid communication; wherein at least one of the stator channels (48) has a transverse channel section (74) opening into the stator face (54) and running transversely with respect to the rotation axis (A); wherein the rotor channel (50) has a bottom (64), which at an intersection end (62) of the rotor channel (50) is inclined with respect to the rotation axis (A), such that the rotor channel (50) elongates an inner surface of the stator channel (48), when the rotor member (24) is in the conducting position.
F16K 3/08 - Robinets-vannes ou tiroirs, c. à d. dispositifs obturateurs dont l'élément de fermeture glisse le long d'un siège pour l'ouverture ou la fermeture à faces d'obturation planes; Garnitures d'étanchéité à cet effet avec éléments de fermeture articulés à pivot en forme de plaques disposées entre l'alimentation et l'évacuation les plaques étant circulaires et pivotant autour de leur centre
F16K 11/074 - Soupapes ou clapets à voies multiples, p.ex. clapets mélangeurs; Raccords de tuyauteries comportant de tels clapets ou soupapes; Aménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont toutes les faces d'obturation se déplacent comme un tout comportant uniquement des tiroirs à éléments de fermeture articulés à pivot à faces d'obturation planes
G01N 30/20 - Injection utilisant une valve d'échantillonnage
8.
LABORATORY AUTOMATION DEVICE WITH TRANSPARENT DISPLAY IN DOOR
A laboratory automation device (10) comprises a workspace (26) with liquid containers (16) and a pipetting arm (20) for moving liquids (18) between the liquid containers (16); a housing (28) enclosing the workspace (26); a door (30) of the housing (28) for accessing the workspace (26), wherein the door (30) comprises a transparent display (32) for displaying information (34) and for allowing a person to view into the workspace (26); and a tracking sensor (36) for tracking an eye position (46) of the person (42). The laboratory automation device (10) is adapted for determining the eye position (46) of the person (42) from sensor data acquired with the tracking sensor (36); and for displaying information (34b) for a notification area (48) in the workspace (26) on the transparent display (32), such that the information (34b) is displayed from a perspective of the person (42) in front of the notification area (48).
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
G06F 3/01 - Dispositions d'entrée ou dispositions d'entrée et de sortie combinées pour l'interaction entre l'utilisateur et le calculateur
A robotic sample handling system (100) may be provided for performing sample handling tasks in a laboratory environment. The system may comprise at least one robotic arm (140), (142) which is controllable to be positioned in a plane parallel to a work area and along a Z-axis perpendicular to the work area, and a controller (180) configured to control the robotic arm to position and operate the robotic arm as part of a sample handling task. The work area may comprise a module (204) for use with one or more of the samples, wherein the module comprises a mechanism which is actuatable by downward force, and wherein the controller is configured to control the robotic arm to actuate the mechanism by pushing downward in Z-direction. For example, the module may be a stand (204) for a sample container which comprise a push-push mechanism which may be operated by the robotic arm to bring the sample in the sample container in a vicinity of an effector, such as a magnet or a heat source.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
B01L 9/00 - Dispositifs de support; Dispositifs de serrage
10.
INCUBATION CASSETTE AND MICROPLATE FOR REDUCING FLUID EVAPORATION OUT OF WELLS OF A MICROPLATE
The invention relates to an incubation cassette (1) for reducing fluid evaporation out of wells of a microplate, comprising a frame (4) for receiving a microplate having wells, wherein the frame (4) has a central first opening (5) surrounded by an inner wall (6) and with dimensions designed for the insertion of a microplate, and the frame (4) also has an outer wall (8) running substantially parallel to the inner wall (6), which connects to the inner wall (6) via an intermediate base, such that a fluid reservoir (9) surrounding the first central opening (5) is formed by by the two walls (6, 8) and the intermediate base to receive a fluid. The incubation cassette (1) also comprises a floating body provided in the fluid reservoir (9), which can be brought into fluid contact with the fluid accommodated in the fluid reservoir (9) in such a way that the floating body experiences a buoyancy force in relation to the fluid level of the fluid accommodated in the fluid reservoir (9).
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
B01L 7/00 - Appareils de chauffage ou de refroidissement; Dispositifs d'isolation thermique
B01L 9/00 - Dispositifs de support; Dispositifs de serrage
G01F 23/68 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme par des flotteurs du type à flotteur libre en utilisant des moyens d'indication actionnés électriquement
A cover (1) for a fluid container (4) comprising an essentially plane cover plate (10) for closing at least one opening of at least one fluid container (4) and at least two ports (11,12), wherein each port (11,12) comprises a trough channel (110,120) extending through the entire thickness of the cover plate (10), wherein a fluid impermeable first membrane (2) is arranged over the entire cross section of the through channel (110) of the first port (11) and wherein an at least gas permeable second membrane (3;30) is arranged over the entire cross section of the through channel (120) of the second port (12).
A displacement device for displacing a displacement volume is provided. The device comprises a first fluid space and a second fluid space. A first piston is arranged in a movable manner within the first fluid space. The first fluid space is connectable to a pipetting device. The first piston is actuatable by an actuation volume of air or of liquid provided by the pipetting device. A second piston is arranged in a movable manner within the second fluid space. The second piston is constructed to displace a displacement volume of a fluid located within the second fluid space when the second piston is actuated. The second piston is actuated in dependence on the first piston that is actuated by the actuation volume. The actuation volume is different from the displacement volume. Furthermore, a pipetting system comprising a displacement device and a method of displacing a displacement volume are provided.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
A cartridge, in particular a disposable cartridge, for use in an electrowetting sample processing system. The cartridge comprises a liquid input port (19') for introducing an input liquid into an internal gap of the cartridge, the input liquid providing for at least one droplet (23), directly or via a liquid separation process within the internal gap, and the internal gap comprising at least one hydrophobic surface, at least one processing zone (78) for processing samples (80) located in the processing zone (78), and a delivery zone (74) for delivering the at least one droplet (23) from the liquid input port (19') to the at least one processing zone (78). The delivery zone (74) is configured to provide a repeating pattern of interacting electrowetting force for simultaneously transporting the at least one droplet (23) within the delivery zone (74).
A cartridge (2) for use in an electrowetting sample processing system, the cartridge (2) comprising at least one inlet port (19') for introducing an input liquid (60, 61) in an internal gap (6) of the cartridge (2), wherein the gap (6) comprises at least one hydrophobic surface (17) and is configured to provide an electrowetting induced movement of a microfluidic droplet (23) of input liquid (60, 61), wherein the input liquid (60, 61) comprises a carrier liquid (60) and a processing liquid (61) and the gap (6) comprises a capture zone that is configured to capture at least a part of the processing liquid (60,61) as a microfluidic droplet (23) by use of electrowetting force and the gap (6) further comprises a transfer zone that is configured to provide a passage for the carrier liquid (60) next to the microfluidic droplet (23), while processing liquid (61) is captured in the capture zone (62).
A cartridge (2) for use in an electrowetting sample processing system, the cartridge comprising one or more inlet ports (19') for introducing an input liquid into an internal gap (6) of the cartridge (2), which comprises at least one hydrophobic surface (17) for enabling an electrowetting induced movement of multiple microfluidic droplets (23) separated from the input liquid. The cartridge (2) further comprises at least one outlet port (80) that is operably connected to the inlet port (19') for providing a liquid flow through the cartridge (2), if a liquid driving force, in particular an electrowetting force or a pressure force, is applied to at least a part of the input liquid.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
16.
CLASSIFYING LIQUID HANDLING PROCEDURES WITH A NEURAL NETWORK
A method for classifying liquid handling procedures comprises, such as pipetting procedures occurring during laboratory automated analyses: receiving measurement data (56) encoding a measurement curve (28, 30, 32) of measurements over time during at least a part of a liquid handling procedure; inputting the measurement data (56) into a neural network (57); and calculating at least one quality value (74, 86) for the liquid handling procedure with the neural network (57). The neural network allows to classify whether a liquid handling procedure is normal or abnormal, e.g. clotting in the line, presence of air or bubbles, etc...
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
A pipette tip extension attachable to a pipette tip comprising a proximal end, a distal end, and an exterior wall extending between the proximal end and the distal end. The exterior wall has an outer side and an inner side and forms at the proximal end a reception aperture for inserting a pipette tip, and at the distal end a dispense aperture. The pipette tip extension further comprises an inner cavity, a distance element connected to the inner side of the exterior wall, and a constriction element.
A pipette tip extension attachable to a pipette tip. The pipette tip extension comprises a proximal end, a distal end, and an exterior wall extending between the proximal end and the distal end. The exterior wall has an outer side and an inner side and forms at the proximal end a reception aperture for inserting a pipette tip. The pipette tip extension comprises further a bottom at the distal end, an inner cavity enclosed by the inner side of the exterior wall and the bottom, and one or more distance elements arranged at the inner side of the exterior wall and protruding into the inner cavity.
A pipette tip extension attachable to a pipette tip. The pipette tip extension comprises a proximal end, a distal end, and an exterior wall extending between the proximal end and the distal end. The exterior wall has an outer side and an inner side and forms at the proximal end a reception aperture for inserting a pipette tip. The pipette tip extension further comprises a bottom at the distal end, an inner cavity enclosed by the inner side of the exterior wall and the bottom, one or more distance elements arranged at the inner side of the exterior wall and protruding into the inner cavity, and a coating for interacting with a fluid present in a fluid uptake area.
A method of treating an isolated area of a sample with a liquid. The method comprises the steps of: - generating an isolated area of a sample by means of a first fluid reservoir, the first fluid reservoir enclosing a distal end of a second fluid reservoir, and the isolated area of the sample being sealed towards the remaining area of the sample, - bringing the isolated area of the sample into fluid connection with the second fluid reservoir, - dispensing a fluid from the second fluid reservoir into the first fluid reservoir, thereby generating a fluid flow on the sample at the isolated area in a first direction, and - aspirating the fluid from the first fluid reservoir into the second fluid reservoir, thereby generating a fluid flow on the sample at the isolated area in a second direction.
A pipette tip extension comprising a proximal end, a distal end, and an exterior wall extending between the proximal end and the distal end. The exterior wall has an outer side and an inner side and encloses an inner cavity which is delimited by the inner side of the exterior wall. The exterior wall forms at the proximal end a reception aperture. The pipette tip extension further comprises one or more distance elements arranged at the inner side of the exterior wall and protruding into the inner cavity.
A centrifugal processing unit (1) for directing the movement of substances within a sample processing cartridge (2) comprises a rotor (3) with an accommodation (31) for receiving the sample processing cartridge (2), the accommodation (31) only allowing a free pivoting motion of the cartridge (2) about a respective pivot axis, wherein each pivot axis is orthogonal to the rotor axis (300) and to a respective force vector of a centrifugal force, a rotor drive to create the centrifugal force, a blocking element (34), allowing the free pivoting motion of the cartridge (2) in a release position and preventing the free pivoting motion in a blocking position, wherein the blocking element (34) is arranged on the rotor (3) spaced apart above the accommodation (31).
B04B 5/04 - Appareils à chambre radiale pour séparer des mélanges essentiellement liquides, p.ex. butyromètres
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
23.
A METHOD FOR DISPENSING OR ASPIRATING A VOLUME OF LIQUID USING A MAGNETIC VALVE AND AS WELL AS A CORRESPONDING DISPENSER/PIPETTING APPARATUS
The present invention relates to a method for dispensing and/or aspirating a volume of liquid (8), in particular employing a method for controlling a magnetic valve (3). The method for dispensing or aspirating a volume of liquid (8) comprises controlling a flow of a system fluid (9) by means of a magnetic valve (3) located between a pressure source (1+, 1-) and a dispenser or pipetting tip (6), and dispensing or aspirating the volume of liquid (8) through an exterior opening (7) of the tip (6) dependent on the flow of the system fluid (9). Controlling the flow comprises determining a flow time in dependence of the volume of liquid (8) to be dispensed or aspirated, and controlling the magnetic valve (3) comprising a solenoid coil (13) and a mobile anchor forming a plunger (14), wherein the plunger (14) is moveable between a closed position (Pc) and an open position (Po), whereby the magnetic valve (3) is held open for the duration of the flow time. Controlling the magnetic valve (3) comprises measuring a capacitance at/of the magnetic valve (3) and determining a position (P) of a plunger (14) based on the measured capacitance. Furthermore, a corresponding dispenser/pipetting apparatus is provided.
H01F 7/18 - Circuits en vue d'obtenir des caractéristiques de fonctionnement souhaitées, p.ex. pour un fonctionnement lent, pour l'excitation successive des enroulements, pour l'excitation à grande vitesse des enroulements
G01F 11/02 - Appareils qu'il faut actionner de l'extérieur, adaptés à chaque opération répétée et identique, pour mesurer et séparer le volume prédéterminé d'un fluide ou d'un matériau solide fluent à partir d'une alimentation ou d'un récipient sans tenir compte avec chambres de mesure qui se dilatent ou se contractent au cours du mesurage
G01F 13/00 - Appareils pour mesurer par volumes et déverser des fluides ou des matériaux solides fluents, non prévus dans les groupes précédents
24.
MIXING APPARATUS, MIXING SYSTEM AND METHOD FOR MIXING SUBSTANCES IN CLOSED CONTAINERS
The invention relates to a mixing apparatus (100), comprising a drive unit (1) having a drive (10) and having a shaft (11) that can be pivoted about a pivot axis (A) and is operatively connected to the drive (10), and comprising a pivotable receptacle (2) which is arranged on the shaft (11), wherein the pivot axis (A) is substantially horizontal, wherein the pivotable receptacle (2) comprises a base plate (20) and at at least one retainer (4, 400) which is spaced apart therefrom, wherein the pivotable receptacle (2) is designed such that holders (3) for containers (7, 70) can be inserted in the direction of the pivot axis (A) between the base plate (20) and the at least one retainer (4, 400).
The invention relates to a device for controlling an optical detection unit using a capacitive fill state measurement in liquid containers (1), comprising a sensor (2) with a measurement electrode (3), a conductive base (4) which is suitable for arranging at least one liquid container (1) or a support unit with at least one receiving area for receiving a liquid container (1), an electronic sensor unit (7) which is connected to the measurement electrode (3) and the base (4) and is designed to determine the capacitance between the measurement electrode (3) and the base (4), an analysis unit (A), and a control unit for controlling the optical detection unit. The measurement electrode (3) is arranged substantially perpendicularly to the base (4), and the control unit is connected to the electronic sensor unit (7) or the analysis unit (A). A control signal can be generated by the electronic sensor unit (7) or the analysis unit (A) depending on the capacitance, and the optical detection unit can be controlled depending on the control signal. The invention additionally relates to a corresponding method for controlling an optical detection unit using a capacitive fill state measurement in liquid containers (1).
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
26.
DEVICES AND METHODS FOR CAPACITIVELY DETECTING FOAM IN LIQUID CONTAINERS
The invention relates to devices and to corresponding methods for capactively detecting foam in liquid containers. A device according to the invention comprises: a sensor (2), which has a measurement electrode comprising a plurality of sub-electrodes (31, 32, 33); a bottom plate (4), which is used as a counter electrode, for arranging a liquid container (1); a sensor electronic unit (7), which is connected to the measurement electrode (3) and the bottom plate (4) and is designed to determine an impedance, in particular a capacitance, between the measurement electrode and the bottom plate (4); and an evaluation unit (A), the measurement electrode being arranged substantially perpendicularly to the bottom plate (4). In an alternative variant of the device according to the invention, the sensor having the measurement electrode is arranged on the bottom plate in a coplanar arrangement, and the liquid container is arranged in a carrier unit, which is used as a counter electrode, the sensor electronic unit being connected to the measurement electrode and the carrier unit. In both variants, the sensor electronic unit (7) comprises a signal generator, by means of which a first signal for exciting the measurement electrode with a first frequency and a second signal for exciting the measurement electrode with a second frequency can be generated. The evaluation unit (A) is designed to detect, on the basis of the impedance, in particular the capacitance, the presence of foam (S) in the liquid container (1) if e.g. the difference between a first measurement signal in the case of excitation of the measurement electrode with the first signal and a second measurement signal in the case of excitation of the measurement electrode with the second signal exceeds a predefined threshold value.
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
27.
CARTRIDGE, ELECTROWETTING SAMPLE PROCESSING SYSTEM AND BEAD MANUPULATION METHOD
The invention concerns a cartridge (2), in particular a disposable cartridge, for use in an electrowetting sample processing system, wherein the cartridge comprises an internal gap (6) with at least one hydrophobic surface (17) for enabling an electrowetting induced movement of a microfluidic droplet (23) that comprises magnetic beads (52) and further comprises a bead accumulation zone (50), into which the microfluidic droplet is transferable by electrowetting force and in which the magnetic beads are exposable to a magnetic force of a bead manipulation magnet. The internal gap (6) comprises a bead extraction opening (60) adjacent to the bead accumulation zone, wherein the bead extraction opening provides a passage from the gap to an exterior space of the cartridge and is configured to removably receive the bead manipulation magnet (62) for enabling an extraction of the magnetic beads from the microfluidic droplet by a removal of the bead manipulation magnet. This enables an efficient and reliable bead extraction out of an electrowetting transportation process.
What is provided is a sample rack (20a) which comprises at least one axially elongate receptacle (21) for a sample tube (30). The sample rack (20a) also comprises a code arrangement (100) arranged on its exterior. This code arrangement contains information relating to the position of the at least one receptacle (21) and also information relating to the total number of receptacles (21) and/or the dimension of the sample rack (20a). Also provided is a sample carrier system (1) which comprises a sample carrier (10) with at least one sample rack receptacle (11) and a set of sample racks (20a, 20b). The sample rack receptacle (11) and the sample racks (20a, 20b) have co-operating means which characterize their belongingness.
The present invention pertains to a pipetting apparatus and method capable of detecting a liquid (9, 9') within an intermediate section (4) of a pipette tube (1) of the pipetting apparatus. The intermediate section (4) is located between an upper section (2) of the pipette tube (1) at which a first electrode (10) is arranged and a lower section (3) at which a second electrode (11) is arranged. The first and second electrodes (10, 11) form a measurement capacitor and are operationally connected to an impedance measurement unit (13), which is adapted to detect whether liquid (9, 9'), such as a portion of a sample liquid (9) or system liquid (9'), is present within the intermediate section (4) based on the measured impedance or change of impedance, e.g. an increase of the capacitance and/or a decrease of the resistance, of the measurement capacitor caused by a presence of the liquid (9, 9') within the intermediate section (4).
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
The invention relates to a gripper comprising a mechanical coupling (1), by which the gripper can be connected to a pipetting tube, at least one fluid duct (2), which extends outwards from the coupling (1) and by which a negative pressure can be transmitted, the gripper comprising at least one suction holder (3) which is connected by the at least one fluid duct (2) to the coupling (1), such that the negative pressure can be transmitted from the coupling (1) to the at least one suction holder (3), and the at least one suction holder (3) is oriented in such a way that an edge (31) of a suction cup (30) of the at least one suction cup (3), in the correct usage position of the gripper, is oriented in a substantially vertical plane.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
G01N 35/04 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse - Détails du transporteur
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
31.
A PIPETTING APPARATUS, A LIQUID HANDLING SYSTEM AND A METHOD OF CONTROLLING PIPETTING
The present invention relates to a pipetting apparatus (10) comprising a pipette tube (14) having a first end provided with an opening for aspirating and/or dispensing of a sample fluid (12) and a second end operationally connected to a pressure generating means (18). The pipetting apparatus (10) comprises at least one measuring unit (26,28) adapted to determine at least one measurement value of the sample fluid based on said aspirating and/or dispensing of the sample fluid (12) and to provide a sample fluid measurement signal representative thereof to an output of the measuring unit (26,28). Said pipetting apparatus (10) further comprises a control circuit (32) operationally coupled to the output of the measuring unit (26,28) and the input of the pressure generating means (18), said control circuit (32) is configured to control said pressure generating means (18) based on the sample fluid measurement signal.
G01N 11/04 - Recherche des propriétés d'écoulement des matériaux, p.ex. la viscosité, la plasticité; Analyse des matériaux en déterminant les propriétés d'écoulement en mesurant l'écoulement du matériau à travers un passage étroit, p.ex. un tube, une ouverture
G01N 11/06 - Recherche des propriétés d'écoulement des matériaux, p.ex. la viscosité, la plasticité; Analyse des matériaux en déterminant les propriétés d'écoulement en mesurant l'écoulement du matériau à travers un passage étroit, p.ex. un tube, une ouverture en chronométrant l'écoulement d'une quantité connue vers l'extérieur
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
G01N 13/02 - Recherche de la tension superficielle des liquides
The invention relates to a microplate processing device (1) comprising: at least one carriage (2, 4) with a first receptacle for microplates, the at least one carriage (2, 4) being movable in a first horizontal direction (X); and at least one lift (3, 5) which is movable in a vertical direction (Z), as a result of which the microplates (6) can be removed from and supplied to the carriage (2, 4), wherein the carriage (2, 4) comprises a through-opening, the periphery of which, in vertical projection, can at least in part be situated horizontally within the periphery of a microplate (6) held in the carriage, and wherein the lift (3, 5) comprises a second receptacle for microplates, the periphery of which, in vertical projection, is situated within the periphery of the through-opening, as a result of which the second receptacle can be moved unhindered through the through-opening in the vertical direction (Z).
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
G01N 35/04 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse - Détails du transporteur
A centrifugal processing unit (1) for directing the movement of substances within a sample processing cartridge (2) comprising a rotor (3) for receiving the sample processing cartridge (2), a rotor drive to create a centrifugal force (F), wherein the centrifugal processing unit (1) further comprising a pivot accommodation (31) and a fix accommodation (32) for receiving the sample processing cartridge (2) that are arranged on the rotor (3), wherein each pivot accommodation (31) being adapted to allow a free pivoting motion of the received sample processing cartridge (2) about a respective pivot axis (310), wherein each pivot axis (310) being orthogonal to the rotor axis (300) and to the respective force vector of the centrifugal force (F).
A centrifugal processing unit (1) for directing the movement of substances within a sample processing cartridge (2), the centrifugal processing unit (1) comprising a rotor (3) with at least one accommodation (33) for receiving the sample processing cartridge (2) a rotor drive for rotating the at least one rotor (3) about a respective rotor axis (300) to create the centrifugal force (F). The centrifugal processing unit (1) further comprising a blocking element (34), allowing the free pivoting motion of the at least one accommodation (33) in a release position, when the rotor (3) is rotated in a first direction and preventing the free pivoting motion in a blocking position, when the rotor (3) is rotated in the opposite direction.
The present invention relates to a measuring device (1) for measuring light signals which are emitted by samples in wells (9) of a microplate (8), in particular a luminescence measuring device for measuring luminescence signals. In this embodiment, the luminescence measuring device (1) comprises a first injector (2) having an injector needle (6) and an outlet end (25) for outputting a reagent into a well (9), and a luminescence optical device (10) having an optical axis (20) which extends essentially perpendicularly with respect to the microplate (8), and a measuring region (23) which encloses the optical axis (20). The injector needle (6) extends under the luminescence optical device (10) and opens with its outlet end (25) into the measuring region of said luminescence optical device (10). The luminescence measuring device (1) also comprises a spray protection (16) which is arranged between the outlet end (25) of the injector (2) and the luminescence optical device (10).
G01N 21/15 - Prévention de la souillure des éléments du système optique ou de l'obstruction du chemin lumineux
G01N 21/25 - Couleur; Propriétés spectrales, c. à d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
The present invention relates to pipette tips (1) for connecting to a pipette tube (2) of a pipetting device, which is used for taking up and dispensing fluids. According to the invention, a pipette tip (1) is shaped as a long tube which forms a pipette body (4), with an opening (5) on one of its ends and the other end is designed to connect to the pipette tube (2). The pipette tip (1) is characterised in that it has an electrode (7) as a volume measuring electrode of a measuring capacitor. The present invention further relates to pipetting devices with such a pipette tip (1), methods for determining the volume of a fluid sample in such a pipette tip (1), methods for recognising such a pipette tip (1) on a pipetting device, methods for producing such a pipette tip (1), uses for such a pipette tip (1) and a set of such pipette tips (1).
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
37.
PIPETTING DEVICE HAVING A PIPETTE TIP DETECTION UNIT AND METHOD FOR DETECTING A PIPETTE TIP ON A PIPETTING DEVICE
The invention relates to a pipetting device having an exchangeable pipette tip (2) for suctioning and discharging fluid volumes for use in automated laboratory devices/systems. In particular, the invention relates to a pipetting device having a pipette tip detection unit and a method for detecting a pipette tip (2) on a pipette device of this type. A detection unit (7) detects whether a pipette tip (2) is connected to the pipette tube (1), or determines a characteristic feature of the connected pipette tip (2). In addition, the pipette tip (2) forms at least one portion of a first electrode, when the pipette tip (2) is in electrical operative contact with the pipette tube (1), and e.g. a pipette tip holder (3), a contact (34) for setting down the pipette tip holder (3) or a working table (5), over which the pipette tube (1) can be moved, forms at least one portion of a second electrode. Alternatively, e.g. one or more neighbouring pipette tips or pipette tube holders form a portion of the second electrode. The pipette tip detection occurs by means of at least one comparison of a measured value of the capacity of the measuring capacitor, formed by the first and second electrode, with one or more reference values.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
38.
METHOD FOR DETERMINING THE POSITION OF A ROBOTIC ARM IN A LIQUID HANDLING SYSTEM, AND A CORRESPONDING LIQUID HANDLING SYSTEM
The present invention relates to a method for determining the position of a robotic arm in an automatic liquid handling system in which a measurement probe (5') with a first electrode is arranged on the robotic arm and, together with a second electrode formed by at least part of a working area (2) or at least part of a container or container carrier, forms a measurement capacitor, which is operatively connected to a measurement unit for measuring an impedance, in particular a capacitance (C), of the measurement capacitor. The method according to the invention has the following steps: a) moving the measurement probe (5') along a first path (p1); b) detecting a first change (ΔC1) in the impedance, in particular in the capacitance (C), of the measurement capacitor at a first point (x1) on the first path (p1); c) defining at least one first reference spatial coordinate (xRef) for a control unit of the robotic arm on the basis of the first point (x1) on the first path (p1). A corresponding automatic liquid handling system and uses of the position-determining method are also specified.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
G01B 7/00 - Dispositions pour la mesure caractérisées par l'utilisation de techniques électriques ou magnétiques
G01B 21/04 - Dispositions pour la mesure ou leurs détails, où la technique de mesure n'est pas couverte par les autres groupes de la présente sous-classe, est non spécifiée ou est non significative pour mesurer la longueur, la largeur ou l'épaisseur en mesurant les coordonnées de points
G01D 5/241 - Moyens mécaniques pour le transfert de la grandeur de sortie d'un organe sensible; Moyens pour convertir la grandeur de sortie d'un organe sensible en une autre variable, lorsque la forme ou la nature de l'organe sensible n'imposent pas un moyen de conversion déterminé; Transducteurs non spécialement adaptés à une variable particulière utilisant des moyens électriques ou magnétiques influençant la valeur d'un courant ou d'une tension en faisant varier la capacité par mouvement relatif d'électrodes de condensateur
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
39.
PIPETTING DEVICE COMPRISING A FLUID VOLUME SENSOR AND FLUID PROCESSING SYSTEM
The invention relates to a pipetting device having tube (1), which has a opening (3) at one end for suctioning or discharging a sample fluid, and which can be operatively connected to a pressure generation means at the other end, wherein a first electrode (5) is formed on the pipetting device, which forms a measuring capacitor together with a second electrode (4') formed by at least one part of the sample fluid (4') that can be received in the tube (1), which measuring capacitor is operatively connected to a measuring unit, and which measuring unit is designed to determine a volume of the suctioned or discharged sample fluid (4') according to the capacity of the measuring capacitor. The invention also relates to a fluid processing system having a pipetting device of this type, as well as a method for determining a processed fluid volume during pipetting with a pipetting device of this type. The invention further relates to uses of this method.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
40.
DETECTION DEVICE AND METHOD FOR DETECTING IDENTIFICATIONS ON AND/OR FEATURES OF LABORATORY OBJECTS
The present invention relates to a detection device for detecting identifications on and/or features of laboratory objects and/or of specimens therein, in particular in connection with automated laboratory systems or installations, such as for example medical, chemical or pharmaceutical analyzers. Serving for example as identifications are barcodes (1) on specimen containers, such as for example specimen tubes (4), reagent troughs or microplates. Used for this are an optical pickup unit (5) and a mirror (7), the distance between which can be varied to detect laboratory objects at different distances in each case from a pickup distance of the optical pickup unit (5) that lies within a depth of field of the optical pickup unit. The mirror (7) serves here for projecting an image of the laboratory object to be detected onto the optical axis (6). As a result, the detection device is greatly simplified, more reliable and less costly in comparison with known devices that use a zoom lens together with an autofocusing device. Also proposed are a laboratory device, such as for example a working area of an automated laboratory system, with a detection device according to the invention and a corresponding detection method.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
G01N 35/04 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse - Détails du transporteur
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
The invention relates to pipette tips (1) for connecting to a pipette tube (2) of a pipetting device, which are used for taking up and discharging fluids. The pipette tip (1) according to the invention is in the shape of an elongated tube forming a pipette body (4), which has an opening (5) at one end and is designed for connecting to the pipette tube (2) at the other end, wherein the pipette tip (1) has a first electrode (7) as a volume measuring electrode of a measuring capacitor and a second electrode (10) as an immersion detector electrode, wherein the first electrode (7) is located on an outer surface of the pipette body (4) or is embedded in the pipette body (4), and the second electrode (10) is located at least partially on an inner surface of the pipette body (4). The invention also relates to pipetting devices having a pipette tip (1) of this type, a method for determining the volume of a sample fluid in a pipette tip (1) of this type, a method for detecting a pipette tip (1) of this type on a pipetting device, a method for producing a pipette tip (1) of this type, uses of a pipette tip (1) of this type, and a set of pipette tips (1) of this type.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
42.
PIPETTING DEVICE, FLUID PROCESSING SYSTEM AND METHOD FOR OPERATING A FLUID PROCESSING SYSTEM
The invention relates to a pipetting device having tube (1), which has an opening (3) at one end for suctioning or discharging a sample fluid, and which can be operatively connected to a pressure generation means at the other end, wherein a first electrode (5) is formed on the pipetting device, which forms a measuring capacitor together with a second electrode (4') formed by at least one part of the sample fluid (4') that can be received in the tube (1), which measuring capacitor is operatively connected to a measuring unit (CAP), and which measuring unit is designed to determine a volume of the suctioned or discharged sample fluid (4') according to the capacity of the measuring capacitor, as well as comprising a first electrical contact (9') which is designed to create an electrical connection with the working fluid (7), wherein the first electrical contact (9') can be electrically connected to the measuring unit (CAP) via a low-resistance converter circuit (WS). The invention further relates to a fluid processing system having a pipetting device of this type.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
The invention relates to a modular pump housing 10 comprising: a pumping chamber 26, substantially cylindrical about a pumping axis and comprising an opening for the insertion of a piston and a base opposite the opening; a face suitable for attaching a fluid interface 12 thereto; and a conduit 29 connecting the base with the face. Using a process according to the invention, the diameter of the chamber can be selected during manufacture without changing the external measurements of the body. A solenoid valve 11 can be attached using the fluid interface. If a solenoid valve is not used, an additional conduit is provided through a sidewall of the chamber for connecting the latter with the fluid interface.
F04B 53/16 - Carcasses d'enveloppe; Cylindres; Chemises de cylindre ou culasses; Connexions des tubulures pour fluide
F04B 53/00 - "MACHINES" À LIQUIDES À DÉPLACEMENT POSITIF; POMPES - Parties constitutives, détails ou accessoires non prévus dans les groupes ou ou présentant un intérêt autre que celui visé par ces groupes
44.
COVER WITH MICRO-CONTAINER INTERFACE FOR COVERING A MICROFLUIDIC GAP
A cover (10) for use in a digital microfluidics system (16) for manipulating samples in liquid portions or droplets is provided. The digital microfluidics system (16) com- prises a first substrate (18) with an array of electrodes (24) and a central control unit (20) for controlling the selection and for providing a number of said electrodes with voltage for manipulating liquid portions or droplets by electrowetting. A working gap (30) with a gap height is located parallel to the array of electrodes (24) and in-between first and second hydrophobic surfaces (26,28) that face each other at least during operation of the digital microfluidics system (16). The cover (10) comprises on one side the second hydrophobic surface (28) and on another side at least one micro-container interface (32), which comprises at least one cone (34). The inner surface of the cone (34) is formed to provide a sealing form fit contact with an outer surface of an inserted micro-container nozzle (36), by which a liquid is transferrable through a fluidic access hole (38) formed into the cover (10) and interconnecting each cone (34) and the gap (30). The cover (10) may be part of a disposable cartridge (14) or may be provided separately.
The invention relates to a device (100) having multiple capacitively working channels, wherein the device (100) comprises a advancable sensor (3.1, 3.2) and a fluid container per channel. The device (100) also comprises a generator (G) for providing a periodic input signal (s,in(t)). In addition, the device (100) comprises one compensation circuit (CT. l, CT.2) per channel, which can be supplied with the periodic input signal (Sin(t)) and which is designed to provide an input signal (Sin1(t), Sin2(t)) at a first output (A. l, A.2) for applying to the sensor (3.1, 3.2) of the channel, wherein the compensation circuit (CT. l, CT.2) comprises a further output (6.1, 6.2) which is designed to provide a signal (s1(t), s2(t)) that can be evaluated to detect a phase boundary.
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
A method comprises the steps of: providing a digital microfluidics system (1) with electrodes (2) attached to a substrate (3) and covered by a hydrophobic surface (5), and a control unit (7) for manipulating liquid droplets (8-1) by electrowetting; providing in close proximity to electrodes (2) a magnetic conduit (9) for directing a magnetic field of a backing magnet to the first hydrophobic surface (5); providing on the hydrophobic surface (5) a liquid droplet (8-1) that comprises magnetically responsive beads (11); moving by electrowetting the liquid droplet (8-1) with the magnetically responsive beads (11) until at least a part of which is placed atop of the magnetic conduit (9); actuating the backing magnet (10) of the magnetic conduit (9) and attracting/concentrating magnetically responsive beads (11); and while actuating the backing magnet (10), moving by electrowetting the liquid droplet (8-1)' with decreased number of magnetically responsive beads (11) away from the specific magnetic conduit (9). Also disclosed are a method for suspending magnetically responsive beads in liquid portions or droplets in digital microfluidics and a disposable cartridge (17) to carry out the methods therein.
In a separation method, a digital microfluidics system (1) with electrodes (2) attached to a substrate or PCB (3), a control unit (7) for manipulating liquid droplets (8-1) by electrowetting, a cartridge accommodation site (18) for taking up a disposable cartridge (17) which comprises a working gap (4) in-between two hydrophobic surfaces (5,6), and a magnetic conduit (9)/backing magnet (10) combination is provided. A barrier element (40) is provided on an individual electrode (2) of the PCB (3) for narrowing the working gap (4). A disposable cartridge (17) is positioned at the cartridge accommodation site (18), its flexible working film (19) touching there and of the barrier element (40) an uppermost surface (22). In the working gap (4) and above a path of selected electrodes (2') a liquid portion (8-2) or liquid droplet (8- 1) with magnetically responsive beads (11) is provided and moved by electrowetting on the electrode path (2') until a magnetic field of the magnetic conduit (9) is reached. The backing magnet (10) is activated before and during moving by electrowetting the liquid portion (8-2) or liquid droplet (8- 1) with the magnetically responsive beads (11) over and/or around the barrier element (40), thereby attracting and substantially removing magnetically responsive beads (11) therefrom. A suspending method, a digital microfluidics system (1), and a disposable cartridge (17) are disclosed too.
A disposable cartridge (1) is configured for use in a digital microfluidics system (3) for manipulating samples in liquid portions or droplets (4). The digital microfluidics system (3) comprises a cartridge accommodation site (2) and a central control unit (7) for controlling the selection of individual electrodes (8) of an electrode array (5) located at said cartridge accommodation site (2) and for providing a number of said electrodes (8) with individual voltage pulses for manipulating liquid portions or droplets (4) by electrowetting. The disposable cartridge (1) comprises a hydrophobic working surface (10) and a rigid cover (11 ) with a second hydrophobic surface (12), the hydrophobic surfaces (10,12) facing each other and being separated or being separable in essentially parallel planes by a gap (13) with a gap height (14).
The invention relates to a method for determining the presence or absence of disposable pipette tips (1) in pipette tip carriers (2) on the work area (3) of a laboratory workstation (4). Each of the pipette tip carriers (2) has a support panel (5) with receiving holes (6) into each of which a disposable pipette tip (1) can be inserted. The laboratory workstation (4) for carrying out the method comprises a robot arm (7) with at least one pipette (8) which is designed to receive and dispose of disposable pipette tips (1). The laboratory workstation (4) comprises a digital camera (9) which is arranged on a support device (10) and is operatively connected to an analyzing unit (11). The work area (3) of the laboratory workstation (4) can be completely imaged in at least one first direction using the digital camera (9). The method has the steps of selecting pipette tip carriers (2) arranged on the work area (3) of the laboratory workstation (4); capturing at least one digital image (12) of each pipette tip carrier (2); defining an initially orthogonal grid (14), which is adapted to the perspective of the digital image (12) and which comprises quadratic grid elements (13), on each of the pipette tip carriers (2); determining pixel areas (15) in a digital image (12), said pixel areas be assignable to individual grid elements (13), wherein each grid element (13) of the defined grid (14) is divided into four quadrants (18); ascertaining the number of pixels with a specified brightness in the specified pixel area (15); and determining whether a disposable pipette tip (1) is present or not in a specific receiving hole (6) of a pipette tip carrier (2).
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
The invention relates to a microplate reader (23) comprising a measurement area (43); an action source (45'); a measuring device (46) for signals caused or generated in or on biological structures in wells (2) of a microplate (3); a transport support (22) for positioning wells (2) of a microplate (3) relative to an optical axis (51) of the measuring device (46); and a controller (50) for the action source (45'), the measuring device (46), and the transport support (22). The microplate reader (23) comprises an incubation device that comprises a frame (4) for receiving a microplate (3) with wells (2), which have well bases, in order to reduce an evaporation of a liquid out of wells (2) of a microplate (3). The frame (4) comprises a first opening (5), which is surrounded by an inner wall (6) and the dimensions of which are designed for inserting a microplate (3), and an outer wall (9), which runs substantially parallel to the inner wall (6) and which adjoins the inner wall (6) via an intermediate base (10) such that a channel (11) that surrounds the first opening (5) is formed by the two walls (6, 9) and the intermediate base (10) in order to receive a liquid adapted to the volume of the microplate wells (2). The incubation device comprises a support surface (7) with a second opening (8), said support surface (7) being designed for carrying an inserted microplate (3), and as a result of the second opening (8), at least one part of the bases of the wells (2) of a microplate (3) inserted into the incubation device is freely accessible through the second opening (8).
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
B01L 9/00 - Dispositifs de support; Dispositifs de serrage
51.
MAGNETIC COUPLING AND MIXING DEVICE, METHOD AND USE
A device (1) for mixing liquids of different viscosity or composition within at least one container (2) is proposed. Each container (2) comprises an inner chamber (3) with an enclosing wall (8) and a bottom (10); an external encasement (6); an interspace (9); and a transfer opening (11) located at a top side of the inner chamber (3). A mixing body (12) located in the inner chamber (3) comprises magnetizable material (13) or a permanent magnet (13') and is spaced apart from the wall (8). The mixing device (1) comprises a coupling body (16) that is movable in the interspace (9) in direction of a longitudinal axis (4) and alongside the wall (8). The wall (8) and the external encasement (6) are transparent to magnetic fields. The coupling body (16) is in magnetic working connection with the at least one mixing body (12). At least one acting permanent magnet (19) or acting magnetizable material (19') located outside the external encasement (6) is in magnetic working connection with the coupling body (16). Moving the at least one acting permanent magnet (19) or acting magnetizable material (19') parallel to the longitudinal axis (4) of the inner chamber (3) simultaneously induces movement of the coupling body (16) inside the interspace (9) and moving the at least one mixing body (12) in the inner chamber (3), thereby mixing of liquids present in the inner chamber (3) of the container (2) is performed.
A sorptive extraction layer for immobilized liquid extraction of target molecules preferably from biological samples is disclosed. The sorptive extraction layer is mainly composed of ethylene-acrylate-copolymer. Preferably, microplate wells are at least partially coated with such a sorptive extraction layer. There is also disclosed a method of extracting target molecules preferably from biological samples. According to this method, immobilized liquid extraction is carried out with a sorptive extraction layer which is mainly composed of ethylene-acrylate-copolymer.
A digital microfluidics system (1) for manipulating samples in liquid droplets within disposable cartridges (2) that comprise a bottom layer (3), a top layer (4), and a gap (6) between the bottom and top layers (3,4) is disclosed. The digital microfluidics system (1) comprises a base unit (7) with cartridge accommodation sites (8); and a central control unit (14) for controlling the selection of individual electrodes (10) of electrode array(s) (9) and for providing these electrodes (10) with individual voltage pulses for manipulating liquid droplets within said cartridges (2) by electrowetting. The digital microfluidics system (1) further comprises board accommodation sites (40) that are located at the cartridge accommodation sites (8) of the base unit (7) and that each can take up a swappable electrode board (41) comprising an electrode array (9) and electrical board contact elements (42) that are individually connected to electrodes (10) of the electrode array (9). Each board accommodation site (40) comprises electrical base unit contact elements (43) that are connected to the central control unit (14) and that are configured to engage with the electrical board contact elements (42) of a swappable electrode board (41) that is placed at said board accommodation site (40). Also disclosed are a selection of disposable cartridges (2), swappable electrode boards (41), and methods for manipulating samples in liquid droplets (23) that adhere to a hydrophobic surface (17).
A liquid droplet manipulation instrument (20) comprises at least one electrode array (21) for inducing a movement of a liquid droplet (19) by electrowetting; a substrate (22) supporting the at least one electrode array (21); and a control unit (23) comprising at least one electrode selector (34) connected with at least one voltage control (29). The at least one electrode selector (34) is accomplished to individually select each electrode (35) of the at least one electrode array (21) and to provide the selected electrode (35) with a voltage controlled by the voltage control (29). The control unit (23) further comprises a central processing unit (36) for controlling the electrode selector (34) and the voltage control (29) to individually select at least one electrode (35) and to provide the at least one selected electrode (35) with an individual voltage pulse which is selected from a group comprising a drive voltage, a ground voltage, and a stop voltage. The control unit (23) is capable to define a path for a guided movement of a liquid portion (19') of a larger volume that covers more than one electrode (35') of one electrode array (21) by the essentially simultaneous selection of a group of two or more subsequent drive electrodes (35') of said electrode array (21) and to provide each one of these selected drive electrodes (35') with a drive voltage pulse along said path. The control unit (23) is accomplished to essentially simultaneously provide a group of two or more electrodes (35) adjacent to or identical with the pulsed drive electrodes (35') with a ground or stop voltage pulse.
An optical measuring apparatus (1) is configured for the analysis of samples contained in liquid drops (2) provided by a liquid handling system (3) that comprises at least one liquid handling tip (4). The optical measuring apparatus (1) comprises a light source (8) for irradiating the liquid drop (2); a detector (10) for measuring sample light (11); an optics system (43) with first optical elements (18) for transmit- ting irradiation light (9), and a processor (13) for accepting and processing the measurement signals (12). The liquid drop (2) is suspended at the liquid handling orifice (6) of the liquid handling tip (4) in a position where the liquid drop (2) is penetrated by a first optical axis (14) defined by the light source (8) and the first optical elements (18). The liquid drop (2) is physically touched only by the liquid handling tip (4) of the liquid handling system (3) and the liquid sample (30) inside the liquid handling tip (4). The optical measuring apparatus (1) comprises means for the mutual adaption of the size and position of the liquid drop (2) with respect to the first optical elements (18) of the optics system (43). An optical measuring method of analyzing samples contained in liquid drops (2) that are provided by liquid handling systems (3) and that are examined using the optical measuring apparatus (1) is disclosed as well.
G01N 21/01 - Dispositions ou appareils pour faciliter la recherche optique
G01N 21/17 - Systèmes dans lesquels la lumière incidente est modifiée suivant les propriétés du matériau examiné
G01N 21/33 - Couleur; Propriétés spectrales, c. à d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p.ex. spectrométrie d'absorption atomique en utilisant la lumière ultraviolette
A cartridge (1) for manipulating samples in liquid droplets with an electrode array (20) when a working film (10) of the cartridge (1) is placed on said electrode array (20). The cartridge (1) comprises a body (2,2',2") with a lower surface (4) and a number of wells (5) configured to hold therein reagents (6) or samples (6'). Each well (5) comprises a bottom opening (73) for releasing a liquid from the well (5). The cartridge (1) comprises a piercable bottom structure (8) configured to seal bottom openings (73) of the wells (5); a working film (10) located below the lower surface (4) of the body (2,2',2"), the working film (10) comprising a hydrophobic upper surface (11); a peripheral spacer (9) connecting the working film (10) to the body (2,2',2"); and a gap (12) between the lower surface (4) of the body (2,2',2") and the hydrophobic upper surface (11) of the working film (10), the gap (12) being defined by the peripheral spacer (9). The cartridge further comprises at least one top piercing system (60), each located within at least one of the wells (5). Each top piercing system (60) comprises a piston (61) and a piercing element (13), the piston (61) being configured to be movable within said well (5) while proving a seal between the piston (61) and the inner wall of the well (5), and the piercing element (13) being configured to pierce the piercable bottom structure (8) for releasing a reagent or sample (6,6') from said at least one well (5) into the gap (12) upon moving the piston (61) within the well (5) toward its bottom opening (73).
A liquid droplet manipulation system (40) comprises a substrate (42) with at least one electrode array (20) and a central control unit (43) for controlling the selection of individual electrodes (44) of the electrode array(s) (20) and for providing the electrodes (44) with individual voltage pulses for manipulating liquid droplets (23) by electrowetting. The liquid droplet manipulation system (40) is configured to receive on top of the electrodes (44) a working film (10) for manipulating samples in liquid droplets (23) with the electrode array(s) (20) when the working film (10) is placed on said electrode array(s) (20). At least one selected individual electrode (44) of the electrode array(s) (20) of the liquid droplet manipulation system (40) is configured to be penetrated by light of an optical detection system for the optical inspection or analysis of samples in liquid droplets (23) that are located on the working film (10). Also disclosed is working film (10) that is to be placed on said electrode array(s) (20) and a cartridge (1) that comprises such a working film (10) for manipulating samples in liquid droplets (23).
Disclosed is a disposable cartridge (2) that comprises a body (47), a bottom layer (3) with a first hydrophobic surface (17'), a top layer (4) with a second hydrophobic surface (17"), and a gap (6) between. The bottom layer (3) is as a flexible film that is sealingly attached its circumference (40) to the top layer (4). The disposable cartridge (2) is devoid of a spacer (5) that is located between the flexible bottom layer (3) and the top layer (4). The top layer (4) comprises loading sites (41) for transferring processing liquids, reagents or samples into the gap (6). When using this disposable cartridge (2), the bottom layer (3) that is configured as a working film for manipulating samples in liquid droplets (23) thereon is placed on an electrode array (9) of a digital microfluidics system (1), which comprises a base unit (7) with a cartridge accommodation site (8), an electrode array (9) located at said cartridge accommodation site (8). The electrode array (9) is supported by a bottom substrate (11), substantially extends in a first plane and comprises a number of individual electrodes (10). The digital microfluidics system (1) also comprises a central control unit (14) for controlling the selection of the individual electrodes (10) of said electrode array (9) and for providing these electrodes (10) with individual voltage pulses for manipulating liquid droplets within the gap (6) of said cartridge (2) by electrowetting. Also disclosed are a selection of disposable cartridges (2) and a method for manipulating samples in liquid droplets (23) that adhere to a hydrophobic surface (17).
A digital microfluidics system (1) is configured for manipulating samples in liquid droplets within a gap (6) of at least one disposable cartridge (2). For providing additional space for collecting and/or storing waste fluids in this digital microfluidics system (1), it is provided at least one waste hollow (50) which is fluidly connected with a gap (6) of a disposable cartridge (2) that comprises a bottom layer (3) with a first hydrophobic surface (17') and a top layer (4) with a second hydrophobic surface (17"). The waste hollow (50) is located next to at least one individual waste electrode (52) that is positioned next to at least one individual electrode (10) of an electrode array 9 of the microfluidics system (1). Each individual waste electrode (52) is operatively connected to a central control unit (14) of the microfluidics system (1) and covers in each case a waste electrode area. The waste hollow (50) covers a waste area that is equal to a multitude of said waste electrode area and said waste hollow (50) has a height (51) that is equal to a multitude of a gap height (53) of the gap (6) of a disposable cartridge (2).
Disclosed is an alternative digital microfluidics system (1) for manipulating samples in liquid droplets within a gap (6) between a first hydrophobic surface (17') of a bottom layer (3) and a second hydrophobic surface (17'') of at least one disposable cartridge (2). Also disclosed are a number of disposable cartridges (2) that comprise a body (47) and/or a rigid cover plate (12). The bottom layer (3) of each disposable cartridge (2) is a flexible film that is sealingly attached to the body (47) or rigid cover plate (12). The disposable cartridge (2) is devoid of a spacer (5) between the first hydrophobic surface (17') and the second hydrophobic surface (17''). When using these disposable cartridges (2), the bottom layer (3) that is configured as a working film for manipulating samples in liquid droplets (23) thereon is placed on an electrode array (9) of a cartridge accommodation site (8) of a digital microfluidics system (1). The electrode array (9) comprises a number of individual electrodes (10). The digital microfluidics system (1) also comprises a central control unit (14) for controlling the selection of the individual electrodes (10) of said electrode array (9) and for providing these electrodes (10) with individual voltage pulses for manipulating liquid droplets within the gap (6) of said cartridge (2) by electrowetting.
A disposable cartridge (2) for use in a digital microfluidics system (1) comprises a bottom layer (3) with a first hydrophobic surface (17'), a rigid cover plate (12) with a second hydrophobic surface (17"), and a gap (6) there-between. The bottom layer (3) is a flexible film laid onto an uppermost surface (52) of a cartridge accommodation site (8) of a digital microfluidics system (1), attracted to and spread over the uppermost surface (52) by an underpressure in an evacuation space (46). The disposable cartridge (2) may be assembled at a cartridge accommodation site (8), a lower surface (48') of the rigid cover plate (12) and the flexible bottom layer (3) then are sealingly attached to each other by mutual contacting. The assembled disposable cartridge (2) is removed from the cartridge accommodation site (8) in one piece that comprises the bottom layer (3), the plane rigid cover plate (12), and the gap (6) that potentially comprises samples and processing fluids. The digital microfluidics system (1) comprises a base unit (7) and a cartridge accommodation site (8) with an electrode array (9) that comprises a number of individual electrodes (10). The digital microfluidics system (1) comprises a central control unit (14) for controlling the selection of the individual electrodes (10) and for providing these electrodes (10) with individual voltage pulses for manipulating liquid droplets within the gap (6) of the cartridge (2) by electrowetting.
A laboratory system (100) and a method for controlling a laboratory system (100) comprising multiple subsystems (SB1 – SBn); each subsystem (SB1 – SBn) being configured to receive and carry out a command (C); and a master control device (1) configured to process command groups (CG) and/or command(s) (C) within the same command group (CG) simultaneously; to process command groups (CG) and/or command(s) (C) of different command groups (CG) sequentially; and within a command group (CG), to process command group(s) (CG) and/or command(s) (C) grouped into a command group (CG) before processing other commands (C) command group(s) (CG) and/or command(s) (C).
G05B 19/042 - Commande à programme autre que la commande numérique, c.à d. dans des automatismes à séquence ou dans des automates à logique utilisant des processeurs numériques
In a method of deducing the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, at least a single stranded target DNA generated from a genomic sample comprising a selected STR, an STR probe (P1,P1'), a reference probe (P2), and two blockers (B1,B2) are provided, and at least three differential hybridization experiments are carried out, based on which the number of STR probe oligonucleotides (P1,P1') bound per target DNA strand in each differential hybridization experiment is determined. The method further comprises the step of comparing these numbers of STR probe oligonucleotides (P1,P1') bound per target DNA strand in the differential hybridization experiments for deducing the number of repeat units in the selected STR on the single stranded target DNA strand. Also disclosed are kits for carrying out STR genotyping by differential hybridization.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
64.
DIGITAL MICROFLUIDICS SYSTEM WITH DISPOSABLE CARTRIDGES
A digital microfluidics system (1) for manipulating samples in liquid droplets within disposable cartridges (2) is disclosed. The disposable cartridges (2) comprise a bottom layer (3), a top layer (4), and a gap (6) between the bottom and top layers (3,4). The digital microfluidics system (1) comprises a base unit (7) with at least one cartridge accommodation site (8); at least one electrode array (9) that comprises a number of individual electrodes (10); at least one cover plate (12) located at the cartridge accommodation site(s) (8); and a central control unit (14) for controlling the selection of the individual electrodes (10) of the electrode array(s) (9) and for providing these electrodes (10) with individual voltage pulses for manipulating liquid droplets within said cartridges (2) by electrowetting. The at least one cover plate (12) of the digital microfluidics system (1) further comprises an electrically conductive material (15) that extends substantially parallel to the electrode array (9) of the cartridge accommodation site (8) the at least one cover plate (12) is assigned to. The electrically conductive material (15) of the cover plate (12) is not connected to a source of a distinct electrical potential during manipulating samples in liquid droplets (23). Also disclosed are a selection of disposable cartridges (2) and a method for manipulating samples in liquid droplets (23) that adhere to a hydrophobic surface (17).
A stem-loop probe for single nucleotide polymorphism (SNP) genotyping of individual SNP nucleic acid target sequences comprises first (1), second (2), and third (3) single stranded nucleic acid portions. The second single stranded nucleic acid portion (2) is located between the first (1) and the third (3) single stranded nucleic acid portions. The first (1) and the third (3) single stranded nucleic acid portions build a double stranded, intramolecular stem (10). The second single stranded nucleic acid portion (2) forms a single stranded oligonucleotide loop (20) with a nucleotide sequence that is complementary to individual SNP nucleic acid target sequences. The nucleotide sequence of the stem-loop probe is chosen such that perfect match probe/target hybrids have a melting point Tm that is at least 5 °C higher than the Tm of mismatched probe/target hybrids. The first (1) and the third (3) single stranded nucleic acid portions of the stem-loop probe comprise a 3' or 5' end configured as an A, T, or C nucleotide, to which A, T, or C nucleotide a non-quenched fluorophore is conjugated. In a method of detecting single nucleotide polymorphism (SNP) in nucleic acid containing samples, a pair of such stem-loop probes for SNP genotyping of two individual SNP nucleic acid target sequences of a sample is utilized. The stem-loop probes (100) comprise the same first (3), second (2), and third (3) single stranded nucleic acid portions and a ratio of perfect match probe/target hybrids to mismatched probe/target hybrids is detected at a certain temperature.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
66.
INLET VALVE FOR CHAMBER SYSTEMS AND SAMPLE CONTAINERS, AND CHAMBER SYSTEMS AND SAMPLE CONTAINERS HAVING SUCH INLET VALVES
The invention relates to an inlet valve (12) for charging an interior (2) of a chamber system (1) or sample container (57) with a liquid (18). The inlet valve (12) comprises a first pipette stem (6) and an inlet opening (5) and is designed to automatically re-close for delivering the liquid (18) by means of a laboratory pipette (19'). The inlet valve (12) comprises a valve body (61) with a blocking element (14), with a pressing part (20) and with a neck (21); a valve space (62) at least partially surrounding the valve body (61) in the area of the neck (21); a spring mechanism (13; 30; 40), and a sealing element (16). The neck (21) connects blocking element (14) and pressing part (20) to each other and comprises an open passage area (23), which is designed at one end to open into the liquid passage (22) of the pressing part (20) and at the other end to open into the valve space (62). The spring mechanism (13; 30; 40) is designed such that it presses a sealing surface (63) of the blocking element (14) against the sealing element (16) in a closure position of the valve body (61). The valve body (61) of the inlet valve (12) is designed such that, under the effect of the pressing part (20), it can be brought into an open position counter to the resistance of the spring mechanism (13; 30; 40). Chamber systems (1) and sample containers (57) having such inlet valves (12) are likewise disclosed and claimed.
The invention relates to a microplate reader (1) and a respective method, wherein the microplate reader (1) comprises at least one measuring device (2',2",2"') and a holding device (5) for accommodating at least one microplate (4) and for positioning the samples-containing wells (3) of this(these) microplate(s) (4) in relation to the at least one measuring device (2',2",2"'). The at least one measuring device (2',2",2"') is used for detecting light which is emitted by samples in wells (3) of a microplate (4) used resp. inserted in this microplate reader (1) and/or which is influenced by samples transilluminated by light in wells (3) of a microplate (4) used resp. inserted in this microplate reader (1). The micro-plate reader (1) according to the invention is characterized in that it comprises a control unit (6) for controlling the composition of a gas atmosphere (7) surrounding the wells (3) containing the samples of microplates (4) used in this microplate reader (1). A respective use is characterized particularly in that living cells are measured in a controlled gas atmosphere (7), wherein the living cells are chosen from a group which comprises microaerophilic, optionally anaerobic and obligatorily anaerobic micro-organisms as well as fungi and eukaryotic cells.
G01N 21/25 - Couleur; Propriétés spectrales, c. à d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p.ex. verrerie de laboratoire; Compte-gouttes
G01N 33/50 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique
C12M 1/04 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p.ex. par des compteurs de colonies
A spread sheare (1), preferably of a robot arm (28) of a liquid handling workstation or robotic sample processor, is accomplished for the variable but equidistant allocation of at least three liquid handling tools (2) in a Cartesian coordinate system of a liquid handling apparatus with an X-, Y-, and Z-axis. The liquid handling tools (2) are orientated substantially parallel to the vertical Z-axis and are aligned in direction of the horizontal Y-axis. The spread sheare (1) comprises pantograph members (3, 15, 15') that form a plurality of parallelograms (4) and that are pivotally connected to each other at nodal points (5, 5', 5", 6), preferably located in all four corners of the parallelograms (4). Each liquid handling tool (2) is attached to a nodal anchor point (6,6') or to a single end point (7) of the spread sheare (1). According to a first aspect of the present invention, each nodal anchor point (6, 6'), to which a liquid handling tool (2) is attached, is rigidly connected via two pantograph members (3) that join at this nodal anchor point (6, 6') to at least five nodal points (5, 5', 5") located on said two pantograph members (3). Preferably, each single end point (7), to which a liquid handling tool (2) is attached, is rigidly connected via the pantograph member (3, 15, 15'), the single end point (7) is located on, to at least four nodal points (5) located on the respective pantograph member (3). According to a second aspect of the present invention, the pantograph members (15, 15') at a first and second extreme position form a triangle structure, at least the nodal points (5) on these extreme positioned pantograph members (15, 15') and the nodal points (5) that are located next to each nodal anchor point (6,6'), to which a liquid handling tool (2) is attached, are each equipped with a bearing (22) for rigidly but pivotably connecting the two pantograph members (3, 15, 15') meeting at the respective nodal points.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
A spread sheare (1), preferably of a robot arm (28) of a liquid handling workstation or robotic sample processor, is accomplished for the variable but equidistant allocation of at least three liquid handling tools (2) in a Cartesian coordinate system of a liquid handling apparatus with an X-, Y-, and Z-axis. The liquid handling tools (2) are orientated substantially parallel to the vertical Z-axis and are aligned in direction of the horizontal Y-axis. The spread sheare (1) comprises pantograph members (3,15,15') that form a plurality of parallelograms (4) and that are pivotally connected to each other at nodal points (5,5',5'',6), preferably located in all four corners of the parallelograms (4). Each liquid handling tool (2) is attached to a nodal anchor point (6,6') or to a single end point (7) of the spread sheare (1). According to a first aspect of the present invention, each nodal anchor point (6,6'), to which a liquid handling tool (2) is attached, is rigidly connected via two pantograph members (3) that join at this nodal anchor point (6,6') to at least five nodal points (5,5',5'') located on said two pantograph members (3). Preferably, each single end point (7), to which a liquid handling tool (2) is attached, is rigidly connected via the pantograph member (3,15,15'), the single end point (7) is located on, to at least four nodal points (5) located on the respective pantograph member (3). According to a second aspect of the present invention, the pantograph members (15,15') at a first and second extreme position form a triangle structure, at least the nodal points (5) on these extreme positioned pantograph members (15,15') and the nodal points (5) that are located next to each nodal anchor point (6,6'), to which a liquid handling tool (2) is attached, are each equipped with a bearing (22) for rigidly but pivotably connecting the two pantograph members (3,15,15') meeting at the respective nodal points.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
70.
DISPENSER AND PROCESS FOR RELEASING FLOWABLE MATERIALS
The invention relates to a dispenser (1) for releasing flowable materials (2, 2') and to a corresponding process. The dispenser (1) comprises at least one line (3) with an inlet end (4) and an outlet end (5) for transporting a flowable material (2) from a vessel (6) to the outlet end (5). The line (3) which can be essentially filled with these materials (2, 2') can be positioned with its inlet end (4) in the flowable material (2, 2') of the vessel (6) or connected to the vessel. A barrier valve (7) is configured to control the release of the flowable material (2, 2') from the outlet end (5), and a control unit (8) controls opening and closure of the barrier valve (7). The inventive dispenser (1) is characterized in that the line (3) comprises an elastic section (9) which can be inserted into the barrier valve (7), said barrier valve (7) being configured as a squeeze valve for stationary occlusion of this elastic section (9) and hence for closure of the line (3); in addition, the control unit (8) for release of a defined, discrete amount of the flowable material (2, 2') into a sample vessel (11) controls an appropriate opening time (t) of the barrier valve (7), said opening time (t) being determined exclusively by the properties of the flowable material (2, 2') to be released and the properties of the line (3) essentially filled with these materials (2, 2').
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
A61M 5/14 - Dispositifs de perfusion, p.ex. perfusion par gravité; Perfusion sanguine; Accessoires à cet effet
71.
METHOD AND DEVICE FOR DETECTING PHASE BOUNDARIES AND CORRESPONDINGLY EQUIPPED LABORATORY DEVICE
The invention relates to devices (100) for detecting a phase boundary in a liquid tank (5.1 - 5.8). The device (100) comprises a sensor (3.1 - 3.8) that can be advanced in the direction of the phase boundary in the liquid tank (5.1 - 5.8). A circuit (13) is provided, which processes an output signal (s(t)) of the sensor (3.1 - 3.8), in order to detect a change in capacitance (dc/dt). The circuit (13) comprises a first channel having a first filter module in order to filter out a first signal (s1(t)), s1d) having a short pulse width from the output signal (s(t)), and a second channel (40) having a second filter module in order to filter out a second signal (s2(t), s2d) having a greater pulse width from the output signal (s(t)). A controller module (8) having a comparator module (11) is further utilized, which determines whether the first signal (s1(t), s1d) reaches a first threshold value. A processing module (12) is further provided, which determines whether the second signal (s2(t), s2d) meets predefined second signal criteria.
G01F 23/26 - Indication ou mesure du niveau des liquides ou des matériaux solides fluents, p.ex. indication en fonction du volume ou indication au moyen d'un signal d'alarme en mesurant des variables physiques autres que les dimensions linéaires, la pression ou le poids, selon le niveau à mesurer, p.ex. par la différence de transfert de chaleur de vapeur ou d'eau en mesurant les variations de capacité ou l'inductance de condensateurs ou de bobines produites par la présence d'un liquide ou d'un matériau solide fluent dans des champs électriques ou électromagnétiques
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
72.
FUNCTIONAL CHECK AND VARIANCE COMPENSATION IN MASS SPECTROMETRY
The invention relates to a test method for checking the function of a mass spectrometer (5) and a method for compensating for ion yield variances in mass spectrometry, characterized in that said method comprises mixing an eluate of a chromatographic separate system (1,2) and a target analyte solution having a known concentration, injecting said mixture into a mass spectrometer (5) having a detector providing a signal, capturing a mass spectrogram based on the detector signal, capturing an integrated mass spectrographic peak area (A) above an integration line (6) and an area (B) below the integrated peak area (A), and forming a mathematical relationship of the areas (A) and (B). The test method thereby further comprises setting a threshold value for said mathematical relationship indicating the limit of the acceptable quality of a mass spectrometric analysis, and accepting or rejecting the mass spectrometric analysis on the basis of a comparison of the mathematical relationship to the set threshold value. The compensation method thereby further comprises evaluating the mass spectrogram for compensating for variances in the ion yield in the detector (5). The invention further relates to devices for performing said test method or compensation method.
A biological sample processing system (1) comprising a container (2) for large volume processing, a flat polymer film (14) having a lower surface ( 15) and a hydrophobic upper surface (16), which is kept at a distance d to the base side (4) of the container (2) by the protrusions (5), the distance d defining at least one gap (17) when the container (2) is positioned on the film (14), and a liquid droplet manipulation instrument (20) comprising at least one electrode array (21) for inducing liquid droplet movements; a substrate (22) supporting the at least one electrode array (21); and a control unit (23) is characterized in that the container (2) and the film (14) are reversibly attached to the liquid droplet manipulation instrument (20), the system (1) thus enabling displacement of at least one liquid droplet (19) from the at least one well (6) through the channel (12) of the container (2) onto the hydrophobic upper surface (16) of the flat polymer film (14) and above the at least one electrode array (21); wherein the liquid droplet manipulation instrument (20) is accomplished to control a guided movement of said liquid droplet (19) on the hydrophobic upper surface (16) of the flat polymer film (14) by electrowetting and to process there the biological sample (9).
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
74.
EXCHANGEABLE CARRIERS PRE-LOADED WITH REAGENT DEPOTS FOR DIGITAL MICROFLUIDICS
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO (Canada)
Inventeur(s)
Wheeler, Aaron R.
Barbulovic-Nad, Irena
Yang, Hao
Abdelgawad, Mohamed
Abrégé
The present invention provides exchangeable, reagent pre-loaded carriers (10), preferably in the form of plastic sheets, which can be temporarily applied to an electrode array (16) on a digital microfluidic (DMF) device (14). The carrier (10) facilitates virtually un-limited re-use of the DMF devices (14) avoiding cross- contamination on the electrode array (16) itself, as well as enabling rapid exchange of pre-loaded reagents (12) while bridging the world-to-chip interface of DMF devices (14). The present invention allows for the transformation of DMF into a versatile platform for lab-on-a-chip applications.
Relates to an interface (1) and its use for the remote control of a computer-controlled liquid handling workstation (2) with a work surface (3), a motorized pipetting robot (5) with at least one pipette (6), and a control computer (10), to which the pipetting robot (5) is connected. A control program (11) activated in this control computer (10) enables the pipetting robot (5) to position the at least one pipette (6) at specific positions (12, 12', 12") on the work surface (3) and to execute a specific action (13) there using the at least one pipette (6). The interface (1) comprises input means (16), visualization means (17), interface software (18), and an electronic memory (19). The visualization means (17) is implemented to visualize the positions (12) at which containers (4) are situated on the work surface (3) of the liquid handling workstation (2) and a selection of the specific actions (13) executable using the pipettes (6). According to the present invention, the interface (1) and the interface software (18) are implemented to visualize as icons (35) at least one pipette (6), optionally selected by the input means (16) or by the interface (1), and at least one designated pipetting position (12,12',12") on a specific container (4), and in addition, to direct the pipetting robot (5) of the computer-controlled liquid handling workstation (2) to position the selected pipettes (6) at the designated pipetting positions (12,12',12") of the specific container (4) immediately after designating the specific position (12,12',12") and prior to executing the selected specific action (13). Alternatively, the interface (1) and the interface software (18) are implemented or used to visualize, in a 2D or 3D simulation, the pipetting robot (5) of the computer- controlled liquid handling workstation (2) and to virtually position the selected pipettes (6) at the designated pipetting positions (12,12',12") of the specific container (4) immediately after designating the specific position (12, 12', 12") and prior to executing the selected specific action (13).
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes ; Manipulation de matériaux à cet effet
The invention relates to a data acquisition method using a laser scanner for the pixel-precise imaging of fluorescent samples which are on object carriers and have been treated with fluorescent dyes. Such a laser scanner comprises a sample table; at least one laser and a first optical system for providing at least one laser beam for exciting the fluorescent samples; a scanner head (50) having an optical deflecting element for scanning this sample in at least one direction of movement (75); a first lens; a second optical system for forwarding emission beams, which are triggered by the laser beams on the sample and are deflected by the first lens and the deflecting element, to at least one detector; a position encoder (91) which emits position encoder signals (92) which indicate the instantaneous location of the scanner head (50) in relation to a zero point; an electronic element for filtering the detector signals (93) with a defined time constant; and an A/D converter for digitizing the filtered detector signals (93). The data acquisition method according to the invention is characterized in that the filtered detector signals (93) from the A/D converter and the position encoder signals (92) are acquired independently, in a parallel manner and continuously by a computer unit or a controller (40) and are related to a common time base (94), wherein the A/D conversion is carried out so often that each pixel (95) of an image is always assigned more than one data point of the A/D converter.
A forensic sample processor (1) for collecting solid forensic samples (2) and/or for extracting biological material from these forensic samples, the forensic sample processor (1) comprises (a) a number of tubes (3), each tube (3) comprising a peripheral wall (4) that encloses a sample space (5) and a bottom (6); and (b) a frame (7) with a footprint (8) that essentially corresponds to the footprint of a standard microplate, the frame (7) comprising a series of parallel, longitudinal walls (9) and parallel, transverse walls (10) that extend essentially perpendicular to each other and to the foot print (8) of the sample processor (1) and that encase compartments (11), within each one of which an individual tube (3) can be closely placed in an upright manner. The forensic sample processor (1) according to the invention is characterized in that each tube (3) comprises an essentially vertical partition wall (13) adjoining to an essentially horizontal partition wall (14), both partition walls (13,14) being sealingly attached to the inner surface of the tube peripheral wall (4) and enclosing an evacuation space (15) with a pouring channel (16) that is open at a top end (17) and that is connected to the tube bottom (6), wherein each vertical partition wall (13) comprises at least one opening (18) that is located near the tube bottom (6).
Relates to a collection/extraction container (1) for collecting solid forensic samples (2) and/or for extracting biological material from these solid forensic samples (2) and its use. The container (1) has a top opening (3) for the insertion of a solid forensic sample (2). The container (1) comprises a basket (7) and an essentially horizontal intermediate floor (8) for the retention of the solid forensic sample (2) during digestion/lysis and extraction, the intermediate floor (8) being permeable to fluids and dividing the inner chamber (5) into an upper sample space (9) and a lower fluid space (10). The container according to the invention is characterized in that is comprises an essentially vertical channel (11), which is accomplished to allow a pipetting needle (12) to be inserted through the sample space (9) and into the fluid space (10) in that the channel (11) has a top orifice (13) that is situated nearby a top opening (3) of the container (1) and in that the channel (11) penetrates the intermediate floor (8) in a bottom orifice (14), wherein the container (1) also comprises a partition wall (15) that at least partially surrounds the channel (11) and that separates the latter from the upper sample space (9).
Relates to a container (1) for providing and transferring liquids, which is implemented as essentially tubular and comprises an intake opening (2) for introducing the liquid, and which comprises an external wall (4) and the floor (5), which are impermeable to liquids. The container according to the present invention is characterized in that this floor (5) comprises an area (6) which, to discharge liquids (7) from the inner chamber (8) of the container (1) into another container (9), may be opened or at least made permeable to these liquids (7). In addition, those containers (1) are disclosed which are characterized in that they are suitable as collecting containers (1') for collecting and providing forensically relevant samples using a sample carrier (3), in that they comprise retention means (13) that are permeable for these liquids (7), using which the sample carrier (3) is retained in these collecting containers (1') when the area (6) of the floor (5) is pierced, made permeable, or opened.
Collecting devices (1) according to the present invention having a laminar sample carrier (2) for collecting and providing biologically relevant samples are characterized in that they comprises a counterpart (4) comprising a through hole (3) which is implemented to support the laminar sample carrier (2) upon punching using a punching tool (5) and upon ejection of a sample carrier portion (6) through the through hole (3) into a container (7) for sample processing. The present invention additionally comprises a collecting set ( 15), which comprises a collecting rack (16) having a number of collecting units ( 13) inserted into compartments (17), a collecting unit (13) being formed by a collecting device (1) and a collecting container (7). The corresponding method for collecting and providing biologically relevant samples and a special punching apparatus (22) for use in this method are also disclosed.
G01N 1/08 - Dispositifs pour prélever des échantillons à l'état solide, p.ex. par coupe à l'outil impliquant un outil d'extraction, p.ex. mèche cylindrique creuse ou trépan
The invention relates to a method for checking whether the transfer of liquid samples has been successful. In said method, a pipetting system or a dispensing system is made to transfer a liquid sample (1) at a specific location (2), and it is verified whether said liquid sample (1) has actually been transferred. The inventive method is characterized in that a distribution image (4) of the intensity of the heat radiation released by said specific location (2) is recorded once the liquid sample (1) has been transferred. Said method can be used in a pipetting system or a dispensing system by making such systems dispense or accept a liquid sample (1) and then checking whether said liquid sample (1) has actually reached or been accepted at the specific location (2). According to the invention, this is achieved by the fact that a distribution image (4) of the intensity at least of the inherent heat radiation released by the specific location (2) is recorded by means of an infrared camera (12) once the liquid sample (1) has been transferred and is compared to a distribution image (4') of the intensity of the heat radiation of said location (2) or the surroundings (5) thereof, which is recorded before the liquid sample (1) is dispensed or accepted.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
G01N 25/48 - Recherche ou analyse des matériaux par l'utilisation de moyens thermiques en recherchant la production de quantités de chaleur, c. à d. la calorimétrie, p.ex. en mesurant la chaleur spécifique, en mesurant la conductivité thermique sur une solution, sorption ou réaction chimique n'impliquant pas une oxydation par combustion ou catalyse
82.
CONDITIONING DEVICE FOR LIQUID HANDLING SYSTEM LIQUIDS
The invention relates to a device (1) for conditioning a system liquid for a liquid handling apparatus, comprising a degassing chamber (2) for degasifying the system liquid, said degassing chamber (2) being provided with a system liquid injection point (3), a system liquid discharge pipe (4), and a gas discharge pipe (5). The inventive device is characterized in that the same further comprises a collection chamber (6) for degasified system liquid, which is spatially separated from the degassing chamber (2) and is connected to the system liquid discharge pipe (4) of the degassing chamber (2) via a circulation pump (7). At least one such device (1) is preferred which is integrated into a mobile station that conditions a system liquid for a liquid handling apparatus and can be connected to a liquid handling apparatus. A liquid handling workstation encompassing at least one liquid handling apparatus for pipetting or dispensing liquid samples with the aid of a system liquid or such a liquid handling apparatus can also comprise at least one inventive device (1).
The invention relates to a device (1) and to a method of displacing liquid containers (2). Said device comprises a support unit (3) which is used to receive the liquid containers (2); a base unit (5) in relation to which the support unit (3) is mounted in an essentially horizontally free oscillating manner by means of connection elements (7); and displacement elements (6) which are used to displace the support unit (3) in relation to the base unit (5). The inventive device (1) is characterised in that the support unit (3) comprises at least one support element (20,21) whereon at least one displacement mass (8) is secured in a displaceable manner. The at least one displacement mass (8) interacts with a displacement element (6) which is secured to said support element (20,21) and can also be displaced thereby. The movements of the at least one displacement mass (8) are displaced counter to the support element (20,21) which supports said mass and the liquid containers which are arranged in the support unit (3).
The invention relates to systems (10; 20; 30) with an emitter (11; 21; 31) for at least partial optical scanning of samples (P, 33.1) and with a receiver (12; 22; 32; 42) for receiving optical signals sent or excited by the emitter (11; 21; 31) and/or a sample (P, 33.1), whereby the receiver (12; 22; 32; 42) operates in a photon counting mode and in an analogue detection mode, the systems (10; 20; 30) comprising a switching means (15; 25; 42.1, 42.5) for automatically switching from one mode to the other. An alternative system according to the invention comprises a similar emitter (31) and receiver (32; 42), whereby the receiver (32; 42) comprises a photon counting sensor (32.1; 42.1, 42.2) that operates in a photon counting mode. This alternative system is characterized in that the receiver (32; 42) comprises at least two avalanche diodes that are simultaneously operated, wherein each one of the avalanche diodes is individually protected from overexposure by an electronics circuit and/or a software algorithm; a beam splitting element (32.4), which un-symmetrically splits a light beam (L1) that arrives from a sample (P, 33.1) into at least two light beams (L2, L3, etc.), which individually are directed to one of the parallel sensing avalanche diodes.
Disclosed are devices (1) and methods for disposing pipette syringes or dispenser syringes (2) in a system (3) used for manipulating liquid samples (4). One inventive device (1) comprises: a robot (5) for orienting pipette syringes or dispenser syringes (2) relative to sample receptacles (6) located in or on the system (3) in an X direction and in a Y direction that extends substantially at a right angle thereto; pipette syringes or dispenser syringes (2) which extend essentially in a vertical direction and can be raised and lowered in a Z direction running substantially perpendicular to the X and Y directions; drive units for moving the robot (5); and processors for controlling the movements and actions of the robot (5) and the pipette syringes and dispenser syringes (2). The inventive device (1) is characterized in that the same comprises at least two blocks (8, 9) which are arranged on a robot (5) arm (7) and on which at least two pipette syringes or dispenser syringes (2) are placed at an axial distance (10) from each other. The axial distance (10) essentially corresponds to the grid space of wells (19) of a microplate (18). At least one of said blocks (8, 9) is configured so as to be individually movable in the X direction and Y direction in order to alternately orient the pipette syringes or dispenser syringes (2) of the two blocks (8, 9) in a staggered manner along a common line (12). The disclosed method makes it possible to re-pipette liquid sample (4) in a time-saving fashion between several sample receptacles, e.g. between small sample tubes and microplates (18) encompassing 24, 96, 384, or 1536 wells.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p.ex. dispositifs d'aspiration, dispositifs d'injection
brevets
