Some embodiments of the disclosure disclose manifolds, microfluidic systems and methods that provide control over fluid flow distribution to an array of bio- scaffolds contained within the manifolds. In some embodiments, multiple perfusates may be injected into the manifold via multiple inlets where the manifold contains a bio-assembly with a substrate having a bio-scaffold disposed thereon. Biological investigations of the perfusates may then be conducted in the vascular components and chambers of the bio-scaffold.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
A method of preparing a structure is provided. The method includes providing an initial structure; casting a first material in one or more void volumes of the initial structure; removing the initial structure from the first material; obtaining a cast structure comprising the first material; coating a second material on the cast structure; casting a third material using the coated cast structure; removing the first material; and obtaining a final structure. In various embodiments, the initial structure can include a first initial structure and a second initial structure and casting a first material in one or more first void volumes of the first initial structure and in one or more second void volumes of the second initial structure. In various embodiments, the method includes assembling the first cast structure and the second cast structure and obtaining an assembled structure comprising the first cast structure and the second cast structure.
A61L 27/18 - Matériaux macromoléculaires obtenus par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone
B33Y 80/00 - Produits obtenus par fabrication additive
B22F 5/00 - Fabrication de pièces ou d'objets à partir de poudres métalliques caractérisée par la forme particulière du produit à réaliser
B29C 39/02 - Moulage par coulée, c. à d. en introduisant la matière à mouler dans un moule ou entre des surfaces enveloppantes sans pression significative de moulage; Appareils à cet effet pour la fabrication d'objets de longueur définie, c. à d. d'objets séparés
3.
METHODS OF CALIBRATION OF A STEREOLITHOGRAPHY SYSTEM
Provided herein is a system for producing a product. The system generally comprises a large-area micro-stereolithography system, an optical imaging system, and a controller in communication with the large-area micro-stereolithography system and the optical imaging system. The large-area micro-stereolithography system is capable of generating the product by optically polymerizing successive layers of a curable resin at a build plane. The controller is capable of analyzing a focus level of the reference target based on the captured image; and based on the analyzing, adjusting a focus property of the projected image beam of the stereolithography system.
A bio-assembly, a kit for a bio-assembly and methods of using the same are provided. The bio-assembly includes a substrate and a bio-scaffold affixed to the substrate. In accordance with various embodiments, a loader plate having a partition or a loader and a plate having a partition are provided. In accordance with various embodiments, the loader plate includes a partition outlet and a partition inlet, wherein the partition outlet and the partition inlet in fluid communication with the gel. In accordance with various embodiments, the partition comprising an internal volume and shaped to receive the bio-scaffold, and the loader includes a loader inlet and a loader outlet in fluid communication with the gel. In accordance with various embodiments, a bio-compatible adhesive positioned between the substrate and the loader plate or plate. In accordance with various embodiments, a fluid mixture is injected into the bio-scaffold.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
5.
MULTI-HEADED AUTO-CALIBRATING BIOPRINTER WITH HEADS THAT HEAT, COOL, AND CROSSLINK
The present invention relates to a three-dimensional bioprinter for printing and/or patterning a single type or multiple types of cells into different geometrical arrangements and other three-dimensional structures, such as tissues. The bioprinter comprises multiple heads that can each be loaded with a different cartridge containing a biomaterial or biological material such as cells in a solution or cells in a hydrogel. Each bioprinter head and cartridge has the ability to heat or cool using Peltier technology. The bioprinter also has the ability to auto calibrate on a bed plate configured to accept a petri dish or microtiter plate.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces
6.
METHODS, DEVICES, AND SYSTEMS FOR THE FABRICATION OF MATERIALS AND TISSUES UTILIZING ELECTROMAGNETIC RADIATION
The present invention provides a three-dimensional bioprinter for fabricating cellular constructs such as tissues and organs using electromagnetic radiation (EMR) at or above 405 nm. The bioprinter includes a material deposition device comprising a cartridge for receiving and holding a composition which contains biomaterial that cures after exposure to EMR. The bioprinter also includes an EMR module that emits EMR at a wavelength of about 405 nm or higher. Also provided is a bioprinter cartridge which contains cells and a material curable at a wavelength of about 405 nm or greater. The cells are present in a chamber and are extruded through an orifice to form the cellular construct.
B41J 2/01 - Machines à écrire ou mécanismes d'impression sélective caractérisés par le procédé d'impression ou de marquage pour lequel ils sont conçus caractérisés par la mise en contact sélective d'un liquide ou de particules avec un matériau d'impression à jet d'encre
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
Powder compositions and articles and methods of forming articles from powder compositions are provided. The powder compositions include at least one powder that preferably includes an amount of one or more branched polymers such as, for example, one or more branched polyamide polymers. The powder composition is preferably capable of being formed, via a layer-by- layer sintering process such as, for example, selective laser sintering, into a three-dimensional article.
C08L 77/00 - Compositions contenant des polyamides obtenus par des réactions créant une liaison amide carboxylique dans la chaîne principale; Compositions contenant des dérivés de tels polymères
B29C 64/153 - Procédés de fabrication additive n’utilisant que des matériaux solides utilisant des couches de poudre avec jonction sélective, p.ex. par frittage ou fusion laser sélectif
Powder compositions and articles and methods of forming articles from powder compositions are provided. The powder compositions include at least one polymer powder and an amount of reinforcing particles having an aspect ratio of preferably at least about 5:1. In a preferred embodiment, the powder composition is capable of being formed, via a laser sintering process, into a three-dimensional article that exhibits one or more desirable mechanical properties in an elevated temperature environment.
C08L 77/00 - Compositions contenant des polyamides obtenus par des réactions créant une liaison amide carboxylique dans la chaîne principale; Compositions contenant des dérivés de tels polymères
B29C 64/153 - Procédés de fabrication additive n’utilisant que des matériaux solides utilisant des couches de poudre avec jonction sélective, p.ex. par frittage ou fusion laser sélectif
B29C 64/20 - Fabrication additive, c. à d. fabrication d’objets en trois dimensions [3D] par dépôt additif, agglomération additive ou stratification additive, p.ex. par impression en 3D, stéréolithographie ou frittage laser sélectif - Détails ou accessoires à cet effet
C08K 3/013 - Charges, pigments ou agents de renforcement
B33Y 70/10 - Composites de différents types de matériaux, p.ex. mélanges de céramiques et de polymères ou mélanges de métaux et de biomatériaux
The present invention provides a low viscosity photocurable composition including (i) a cationically curable component (ii) a free radically active component (iii) an antimony- free cat ionic photoinitiator and (v) a free radical photoinitiator. The photocurable composition can be cured using rapid prototyping techniques to form three-dimensional articles which can be used in various aerospace and investment casting applications.
C08L 33/04 - Homopolymères ou copolymères des esters
B29C 64/124 - Procédés de fabrication additive n’utilisant que des matériaux liquides ou visqueux, p.ex. dépôt d’un cordon continu de matériau visqueux utilisant des couches de liquide à solidification sélective
C08F 2/50 - Polymérisation amorcée par énergie ondulatoire ou par rayonnement corpusculaire par la lumière ultraviolette ou visible avec des agents sensibilisants
10.
PHOTOCURABLE COMPOSITION FOR PRODUCING CURED ARTICLES HAVING HIGH CLARITY AND IMPROVED MECHANICAL PROPERTIES
A photocurable composition comprising:(a) a cationically curable component preferably formed from one or more epoxy compound(s), (b) an acrylate component having no or a low amount of hydroxy groups preferably dipentaerythritol hexaacrylate, (c) a polyol component, preferably a polyether polyol, (d) a cationic photoinitiator; and (e) a free radical photoinitiator. The composition may produce when cured three-dimensional articles with high clarity and improved mechanical properties.
G03F 7/00 - Production par voie photomécanique, p.ex. photolithographique, de surfaces texturées, p.ex. surfaces imprimées; Matériaux à cet effet, p.ex. comportant des photoréserves; Appareillages spécialement adaptés à cet effet
G03F 7/038 - Composés macromoléculaires rendus insolubles ou sélectivement mouillables
11.
LASER SINTERING PROCESSES USING THERMOPLASTIC COMPOSITIONS
A method of laser sintering a thermoplastic composition to provide an article of manufacture having a plurality of sintered layers is disclosed. The thermoplastic composition has a relatively high melting point and a small particle size. The articles of manufacture have a high resolution and excellent durability and strength.
B29C 64/153 - Procédés de fabrication additive n’utilisant que des matériaux solides utilisant des couches de poudre avec jonction sélective, p.ex. par frittage ou fusion laser sélectif
C08L 53/00 - Compositions contenant des copolymères séquencés possédant au moins une séquence d'un polymère obtenu par des réactions ne faisant intervenir que des liaisons non saturées carbone-carbone; Compositions contenant des dérivés de tels polymères
brevets
