Roche Diagnostics Corporation

United States of America

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IPC Class
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides 1
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids 1
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Quantitative multiplex PCR with high dynamic range

      
Application Number 10300576
Grant Number 07118867
Status In Force
Filing Date 2002-11-19
First Publication Date 2003-11-20
Grant Date 2006-10-10
Owner
  • Roche Diagnostics Corporation (USA)
  • Idaho Technology, Inc. (USA)
Inventor
  • Tabiti, Karim
  • Betzl, Gisela
  • Soong, Richie
  • Rasmussen, Randy P.
  • Desilva, Deepika Marine
  • Ward, John G.
  • Millward, Hallegh Page

Abstract

The invention provides a method for quantitative multiplex PCR of two targets whereby one target is present in at least 100-fold molar excess over that of the other target. The method further comprises the addition of a thermostable DNA polymerase with a final concentration of at least 0.5 units/μl.

IPC Classes  ?

  • C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
  • C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides