Abstract

A computer-implemented method for detecting at least one analyte in a sample with a laser desorption mass spectrometer (220) is disclosed. The method comprises: a) at least one imaging step comprising imaging at least one reflective target (128) by using at least one imaging device (235), wherein the sample comprising the at least one analyte is applied to the reflective target (128); b) at least one sample recognition step comprising localizing at least one sample re- gion on the reflective target (128); and c) at least one analyte detection step comprising detecting the at least one analyte in the sample using surface assisted laser desorption ionization mass spectrometry (SALDI-MS) with the laser desorption mass spectrometer (220), wherein laser ir- radiation is applied to the reflective target (128) by using at least one laser source (222) of the laser desorption mass spectrometer (220) such that at least one ion of the at least one analyte is generated which is detected by using at least one of a mass analyzing unit (224) or an ion-mobility spectrometry device of the laser de- sorption mass spectrometer (220), wherein the laser irradiation is steered on the localized sample region by using at least one control device (237).

IPC Classes  ?

• H01J 49/00 - Particle spectrometers or separator tubes
• H01J 49/16 - Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
• H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Abstract

An in-vitro diagnostic (IVD) analyzer (200) comprising at least one sensor (212) located in a flow-through sensor path (211) of detecting unit and requiring at least one oxygenated calibration fluid (221', 222') for calibration is herein disclosed. The IVD analyzer (200) further comprises a fluid-supply unit (220) comprising at least one deoxygenated calibration fluid (221, 222), a fluid-selection valve (230) and at least one oxygenation tubing (215, 216) having two ends connected to the fluid-selection valve (230) as a loop, wherein the oxygenation tubing (215, 216) comprises oxygen-permeable walls, and wherein the IVD analyzer (200) further comprises a pump (240) and a controller (250) configured to control the pump (240) and the fluid-selection valve (230) for transporting deoxygenated calibration fluid (221, 222) into the oxygenation tubing (215, 216), to wait a predetermined time required for oxygenation of the deoxygenated calibration fluid (221, 222) via oxygen uptake from ambient air through the tubing walls, and to transport the thereby obtained oxygenated calibration fluid (221', 222') into the sensor path (211) for calibration of the at least one sensor (212). A respective automatic method of calibrating at least one sensor (212) is herein also disclosed.

IPC Classes  ?

• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
• G01N 33/96 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
• G01N 27/327 - Biochemical electrodes

Abstract

Computer-implemented methods for analysing a medical image are provided, the method comprising: obtaining a medical image; inputting the image into a machine learning model, the machine learning model trained with training medical images, by: obtaining a plurality of smaller image segments from the training medical images; obtaining an image embedding for each image segment; and processing the embeddings using one or more attention mechanisms comprising a B-cos transform. Related methods, products and systems are described.

IPC Classes  ?

• G06N 3/045 - Combinations of networks
• G06N 3/048 - Activation functions
• G06N 3/09 - Supervised learning
• G06N 3/096 - Transfer learning
• G06T 7/00 - Image analysis
• G06T 7/10 - Segmentation; Edge detection
• G06V 10/82 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks

Abstract

Computer-implemented methods of providing a disease diagnosis or prognosis for a patient are described. These comprise generating individual networks, each individual network comprising a plurality of nodes and edges between pairs of the nodes, wherein each node is indicative of a biological factor in biological data for an individual, and each edge is indicative of a relationship between a pair of biological factors corresponding to the nodes that the edge connects for the respective individual; determining the value of one or more similarity metrics between one or more individual networks generated for the patient and one or more individual networks generated for other individuals in the plurality of individuals; and predicting a diagnosis or prognosis for the patient using a machine learning model that takes as input the values of one or more similarity metrics.

IPC Classes  ?

• G16B 40/20 - Supervised data analysis
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems

Abstract

Methods of sequencing by expansion and related improvements to the sequencing of surrogate polymers in a nanopore are described. The surrogate polymer is formed from a template nucleic acid molecule. A surrogate polymer includes multiple units. Each unit includes a reporter code portion. The reporter codes correspond to the different nucleotides. surrogate polymers may get stuck in the nanopore. Embodiments described herein address these stuck surrogate polymers. In order to allow for multiple reads on the surrogate polymer, a processive consensus technique can be applied. The surrogate polymer may be moved a few units forward and then fewer units backward so that some of the same reporter codes are identified again. This method allows for multiple reads of the same reporter codes. The surrogate polymer eventually passes through the nanopore in the forward direction. Periodically, higher clearing voltages may be applied to clear any stuck surrogate polymer in the nanopore.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing

Abstract

A diagnostic device (110) for monitoring at least one body tissue (134) of a patient is disclosed. The diagnostic device (110) comprises: a. at least one bracelet (112) configured to be strapped around a body part (132) of the patient; b. at least one electromechanical actuator (114) configured for actively varying a circumference of the bracelet (112); c. at least one measurement unit (126) configured for determining at least one item of information on an electrical power applied to the electromechanical actuator (114) and at least one item of circumference information on the circumference of the bracelet (112); and d. at least one evaluation unit (128) configured for determining at least one item of information on a status of the body tissue (134) from the item of information on the electrical power applied to the electromechanical actuator (114) and the item of circumference information, wherein the evaluation unit (128) is configured for determining a point of contact at which the circumference of the bracelet (112) corresponds to the circumference of the body part. Further, a method of monitoring at least one body tissue (134) of a patient is disclosed.

IPC Classes  ?

• A61B 5/107 - Measuring physical dimensions, e.g. size of the entire body or parts thereof
• A61B 5/05 - Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves
• A61B 5/00 - Measuring for diagnostic purposes ; Identification of persons
• A61B 5/0205 - Simultaneously evaluating both cardiovascular conditions and different types of body conditions, e.g. heart and respiratory condition

Abstract

This automated analysis device comprises an analyzing unit 40 for analyzing a sample, and a control device 20 for controlling operations of each mechanism of the analyzing unit 40, wherein the control device 20 calculates a waiting time that a user should wait until the sample or a consumable required to analyze the sample is replaced, on the basis of a time at which the sample or the consumable is to be used last, in an analysis schedule created at a time point at which a replacement request for the sample or the consumable is accepted. By this means, the present invention provides an automated analysis device, and a method for operating the automated analysis device, capable of improving work efficiency.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

The present disclosure is directed to compositions and kits for PCR amplification. The present disclosure is also directed to methods of amplifying nucleic acid molecules to improve upon uniformity of coverage and/or to reduce GC bias during downstream sequencing operations.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Abstract

Methods for the rapid detection of the presence or absence of Monkeypox Virus (MPXV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the MPXV F3L gene and the MPXV B21R gene, along with kits are provided that are designed for the detection of MPXV.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

The present invention provides a method comprising the steps of a) providing a nucleic acid comprising 5fC, 5hmC, or 5caC, b) providing one reactant comprising two reactive groups wherein the first reactive group is capable of reacting with the formyl hydroxymethyl or carboxyl group, and the second reactive group is a nucleophilic group, c) reacting said first reactive group with the formyl, hydroxymethyl or carboxyl group thereby resulting in a modified 5fC, 5hmC, or 5caC, d) reacting said second reactive group with the C6 position of said modified 5fC, 5hmC, or 5caC, thereby obtaining a bicyclic or tricyclic molecule comprising a 5,6-di-hydro Cytosine entity, and e) deaminating said 5,6-di-hydro Cytosine entity to a 5,6 di-hydro-Uracil entity.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

A biomedical knowledge graph system, the system including a computer database of records, the records comprising nodes of biomedical entities and connections between the entities representing biomedical relationships. One or more processors are programmed and configured to extract data from a plurality of data sources, determine biomedical entities and relationships between the entities based on analyzing the data, wherein analyzing the data comprises searching for predetermined identifiers or patterns in the data. Based on the determined biomedical entities, assigning each biomedical entity to a cluster of biomedical entity types. The one or more processors are configured to identify a context for each of the identified biomedical entities based on the assigned cluster and based on elements of the expression of the biomedical data within which the entity is expressed. Based on the identified context and type of the biomedical entity, incorporating records of nodes and connections between nodes into the knowledge graph, the nodes representing biomedical entities and the connections representing biomedical relationships between the entities structured according to the predefined schema.

IPC Classes  ?

• G16B 50/10 - Ontologies; Annotations
• G16B 50/20 - Heterogeneous data integration
• G16B 45/00 - ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks
• G06F 16/332 - Query formulation

Abstract

The present invention relates to a method for assessing whether a subject has Polycystic Ovarian Syndrome (PCOS) or is at risk of developing PCOS, to a method of selecting a patient for therapy of PCOS, to a method for monitoring PCOS progression or for monitoring response to treatment and to a computer-implemented method for assessing a subject with suspected PCOS, by determining the amount or concentration of Leukotriene A4 Hydrolase (LTA4H) in a sample of the subject.

IPC Classes  ?

• G01N 33/88 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving prostaglandins

Abstract

The present invention relates to a method for assessing whether a subject has Polycystic Ovarian Syndrome (PCOS) or is at risk of developing PCOS, to a method of selecting a patient for therapy of PCOS, to a method for monitoring PCOS progression or for monitoring response to treatment and to a computer- implemented method for assessing a subject with suspected PCOS, by determining the amount or concentration of Meteorin-like protein (METRNL) in a sample of the subject.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Abstract

The present invention relates to a method for assessing whether a subject has Polycystic Ovarian Syndrome (PCOS) or is at risk of developing PCOS, to a method of selecting a patient for therapy of PCOS, to a method for monitoring PCOS progression or for monitoring response to treatment and to a computer- implemented method for assessing a subject with suspected PCOS, by determining the amount or concentration of Fibroblast Growth Factor-Binding Protein 1 (FGFBP1) in a sample of the subject.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Abstract

The present invention relates to a method for diagnosing Polycystic Ovarian Syndrome (PCOS) in a subject, said method comprising the steps of a) determining the amount or concentration of total LTA4H in sample from the subject, b) determining the amount or concentration of METRNL in a sample from the subject, c) calculating a score of the amounts or concentration determined in steps a) and b), d) comparing the calculated score with a reference score, and e) diagnosing PCOS in a subject.

IPC Classes  ?

• G01N 33/88 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving prostaglandins

Abstract

The present invention relates to a method for diagnosing Polycystic Ovarian Syndrome (PCOS) in a subject, said method comprising the steps of a) determining the amount or concentration of total FGFBP1 in sample from the subject, b) determining the amount or concentration of METRNL in a sample from the subject, c) calculating a score of the amounts or concentrations determined in steps a) and b), d) comparing the calculated score with a reference score, and e) diagnosing PCOS in a subject.

IPC Classes  ?

• G01N 33/563 - Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Abstract

The disclosure refers to a method of operating a laboratory sample distribution system having a plurality of carriers (4) configured to carry one or more sample containers containing a sample to be analyzed by laboratory devices (3); a transport plane (1) assigned to the laboratory devices (3) and providing support to the plurality of carriers (4); and a driving device (13) configured to move, in response to driving control signals, the plurality of carriers (4) between plane positions (5) provided on the transport plane (1). The method comprises: prior to moving the carriers (4) on the transport plane (1), pre-determining off-line routes (6) on the transport plane (1) by one or more processors of a data processing device, the pre-determining comprising: determining a model representing the transport plane (1) with plane locations (5') and location-to-location movements between plane locations (5') associated to the plurality of carriers (4) calculating an optimized set of off-line routes between pairs of plane locations from the plurality of plane locations (5') using the model, the calculating comprising solving an optimization problem in which routes between the pairs of plane locations are simultaneously optimized; and providing the optimized set of off-line routes as off-line routes (6) on the transport plane (1); and controlling the driving device (13) such that the carriers (4) are moved along the pre-determined off-line routes (6) on the transport plane (1). Furthermore, a laboratory sample distribution system, and a laboratory automation system are provided.

IPC Classes  ?

• G06Q 10/0835 - Relationships between shipper or supplier and carriers
• B65G 54/02 - Non-mechanical conveyors not otherwise provided for electrostatic, electric, or magnetic
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system

Abstract

The disclosure refers to a method of operating a laboratory sample distribution system having: a plurality of carriers (4) having a number of n (n>3) carriers (4) each configured to carry one or more sample containers containing a sample to be analyzed by laboratory devices (3); a transport plane (1) configured to support to the plurality of carriers (4), wherein the transport plane (1) comprises a plurality of interconnected transport modules comprising a plurality of plane fields (5); and a driving device (13) configured to control movement of the plurality of carriers (4) along individual routes between the plurality of plane fields (5). The method com- prises: moving the plurality of carriers (4) along the individual routes on the transport plane (1), wherein the moving, for each carrier, comprises executing at least once steps of reserving a route segment along the individual route, the route segment being provided by one or more plane fields of the plurality of plane fields (5), and moving the carrier (4) along the route seg- ment; and preventing, for the plurality of carriers (4), a deadlock arrangement on the transport plane in which the plurality of carriers (4) block each other from further movement along the individual routes (6). The preventing is further comprising: determining, at a present operation time, a potential deadlock arrangement for the plurality of carriers (4) on the transport plane (1) at a future operation time, wherein the potential deadlock arrangement is assigned a number of n deadlock plane fields occupied by the plurality of carriers (4) in case of the potential dead- lock arrangement; for a first carrier from the plurality of carriers (4) moving along a first individ- ual route, reserving a first route segment ending with a first end plane field; and assigning a non-reserve flag to a next plane field which is next to the first end plane field along the first individual route. Further, a laboratory sample distribution system, and a laboratory automation system are provided.

IPC Classes  ?

• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
• G06Q 10/0835 - Relationships between shipper or supplier and carriers
• B65G 54/02 - Non-mechanical conveyors not otherwise provided for electrostatic, electric, or magnetic

Abstract

A method for quality check of at least one Liquid Chromatography-Mass Spectrometry (LC-MS) measurement on a sample comprising an analyte of interest and a defined amount of at least one internal standard is proposed. The method comprises the following steps a) (120) determining an information (peakAreaaqn) about an analyte signal and an information (peakAreatqn) about an internal standard signal of the LC-MS measurement; b) (122) determining at least one monitoring parameter by using the information about the analyte signal and the information about the internal standard signal by using at least one processing device (114), wherein the monitoring parameter comprises a minimal limit for the internal standard signal for said analyte signal of said sample; c) (124) comparing the information about the internal standard signal to the monitoring parameter by using the processing device (114), wherein the LC-MS measurement is flagged by using the processing device (114) as to fulfil the quality check in case the information about the internal standard signal is greater or equal to the monitoring parameter or otherwise as failed.

IPC Classes  ?

• G01N 30/04 - Preparation or injection of sample to be analysed
• G01N 30/86 - Signal analysis

Abstract

The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. This present disclosure is also directed to a faster and easier method of target capture using primer extension reactions that can improve ease of use, turnaround time, and variant allele specificity by designing target enrichment primers to specifically enrich library fragments based on the relative location of the variant base(s) in the primer, the utilization of polymerases with better priming specificity, designing the variant bases in the capture primer, designing the variant bases in the release primer, and/or designing variant specific primers to the both the plus and minus strands of the target library fragment.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism

Abstract

A door mechanism device (110) for a door (100) for a transport apparatus (500) for trans- porting a sample container carrier is disclosed. The door mechanism device (110) comprises at least a first fixation bracket (112) configured for mounting the door mechanism device (110) to a frame of the transport apparatus, at least a first upper lever (114), at least a first lower lever (116), at least a first door mount (120) configured to be mounted to a cover (102) of a door (100) of the transport apparatus. The first upper lever (114) and the first lower lever (116) are rotatably mounted to the first fixation bracket (112) and the first door mount (120). The first upper lever (114) and the first lower lever (116) are rotatable around lever axes (122) such that the first door mount (120) is movable between a first position and a second position with the first door mount (120) substantially maintaining its orientation within a plane perpendicular to the lever axes (122). Further, a door (100) for a transport apparatus for transporting a sample container carrier and a transport apparatus (500) for transporting a sample container carrier are disclosed.

IPC Classes  ?

• E05D 15/40 - Suspension arrangements for wings supported on arms movable in vertical planes
• E05D 15/46 - Suspension arrangements for wings supported on arms movable in vertical planes with two pairs of pivoted arms
• E05F 5/00 - Braking devices, e.g. checks; Stops; Buffers
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

The present disclosure relates, in general, to novel and easily accessible fluorescent compounds with large Stokes shift (LSS) and thermostable fluorescence for expanding the multiplexing capabilities of fluorescence-based nucleic acid detection technologies. Moreover, conjugates, probes and FRET pairs comprising these fluorescent compounds as well as methods for amplification and detection of a target nucleic acid utilizing these fluorescent compounds and methods of labeling are also provided.

IPC Classes  ?

• C09B 11/24 - Phthaleins containing amino groups
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

Abstract

A method of operating a distribution system (110), wherein the distribution system (110) comprises - a number of carriers (112) configured for carrying one or more objects (114), - a transport plane (122) configured for supporting the carriers (112), wherein the transport plane (122) comprises a plurality of transport modules (124), wherein a grid (126) of logical positions (128) is defined on the transport plane (122), - a drive system (130) configured for moving the carriers (112) on the transport plane (122) between the logical positions (128), - a control system (136) configured for controlling the drive system (130), wherein the control system (136) comprises a routing system (138) configured for calculating routes for the carriers (112), wherein the method comprises the steps: a) defining a global pattern of safe points (148) and applying the global pattern on the transport plane (122) by using the routing system (138), wherein safe points (148) are logical positions (128) selected in view of a range of motion for a carrier (112) occupying said logical position (128) such that on the safe points (148) a carrier (112) can be placed and can be moved away again, wherein the global pattern is applied onto the transport plane (122) independently of module boundaries; b) calculating partial routes for the carriers (112) so that an end position of each partial route is either one of the safe points (148) or has a free path to one of the safe points (148) to be reachable in the next partial route by using the routing system (138). Further, a distribution system (110) and a computer program and a computer-readable storage medium for performing the method according to the present invention are disclosed.

IPC Classes  ?

• G01N 15/04 - Investigating sedimentation of particle suspensions
• B65G 35/06 - Mechanical conveyors not otherwise provided for comprising a load-carrier moving along a path, e.g. a closed path, and adapted to be engaged by any one of a series of traction elements spaced along the path
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G06Q 10/04 - Forecasting or optimisation specially adapted for administrative or management purposes, e.g. linear programming or "cutting stock problem"
• G06Q 10/08 - Logistics, e.g. warehousing, loading or distribution; Inventory or stock management
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system

Abstract

The present disclosure relates, in general, to the methods for the rapid detection of the presence or absence of Lymphogranuloma Venereum (LGV)-causing serovars of Chlamydia trachomatis in a biological or non-biological sample. The methods can include performing an amplification step, a hybridization step, and a detection step. Furthermore, oligonucleotide primers and probes targeting the pmpH gene for the L serovars of Chlamydia trachomatis, along with kits are provided that are designed for the detection of L serovars.

IPC Classes  ?

• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

A distribution system (110) and a method for distributing a plurality of carriers (114) using the distribution system (110) are disclosed. The distribution system (110) comprises: - at least one transport plane (118) comprising logical positions (120); - a plurality of carriers (114) for transporting objects (122); - at least one drive system (126) for moving the carriers (114) on the transport plane (118) between the logical positions (120); and - at least one control system (128) configured for controlling the carriers (114) to move on a planned route from a start position to a final destination position on the transport plane (118), wherein the planned route comprises partial routes (144), wherein the control system (128) comprises at least one routing system (130) configured for calculating routing plans for carriers (114) on the transport plane (118) by modeling the transport plane (118) with graph of nodes (132), wherein the routing system (130) is configured for calculating the routing plans considering moving time periods (160) and waiting time periods (162), wherein the routing system (130) is configured for assigning waiting time periods (162) for carriers (114) depending on a reservation of logical positions (120) of the partial routes (144) of other carriers (114), wherein, if a carrier (114) experiences a time delay (158) during execution of a move, the routing system (130) is configured for shifting the experienced time delay (158) to at least one upcoming waiting time period (166) of a carrier (114).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
• B65G 35/06 - Mechanical conveyors not otherwise provided for comprising a load-carrier moving along a path, e.g. a closed path, and adapted to be engaged by any one of a series of traction elements spaced along the path
• G06Q 10/04 - Forecasting or optimisation specially adapted for administrative or management purposes, e.g. linear programming or "cutting stock problem"
• G06Q 10/08 - Logistics, e.g. warehousing, loading or distribution; Inventory or stock management

Abstract

The present invention relates to methods of diagnosing whether a subject has endometriosis, to methods of classifying the stage of endometriosis, to methods of determining the therapeutic effect of a treatment regimen for endometriosis, and methods of monitoring endometriosis progression in a subject, by determining the amount or concentration of c-Kit in a sample of the subject, and comparing the determined level to a reference value.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Abstract

The invention includes improved methods and compositions for reduction of a C5-C6 double bond of a cytosine. In particular, the improved methods and compositions for reduction of a C5-C6 double bond of a cytosine is via enzymatic means, not via chemical means. In particular, the disclosure is directed to methods of converting 5,6-dihydro-fC (fC) and/or 5,6-dihydro-caC to 5,6-dihydro-U (DHU). In particular, the disclosure is directed to methods of converting 5fC and/or 5caC to DHU. In addition, the disclosure is directed to methods for detection of epigenetic cytosine modification, particularly cytosine methylation, using ene reductases to reduce the C5-C6 double bond of cytosine.

IPC Classes  ?

• C12Q 1/6804 - Nucleic acid analysis using immunogens
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism

Abstract

The present invention provides novel primers in which a ferrocene label is attached to the primer. The ferrocene label is incorporated into the amplification product. When the amplification product incorporating the ferrocene label is denatured, it can bind to a capture probe and the presence or absence of the ferrocene label can be detected via electrochemical detection. The system avoids the use of a signal probe in a sandwich assay as historically used during electrochemical detection.

IPC Classes  ?

• C12Q 1/6816 - Hybridisation assays characterised by the detection means
• C12Q 1/6825 - Nucleic acid detection involving sensors

Abstract

A method for multiple reaction monitoring using a mass spectrometry device (106) is proposed. The method comprises the following steps: i) (128) measuring, by using the mass spectrometry device (106), multiple reaction monitoring transitions of quantifier and qualifier of both an internal standard and an analyte using staggered- multiple reaction monitoring, wherein the staggered-multiple reaction monitoring comprises at least three multiple reaction monitoring channel groups, wherein one of the multiple reaction monitoring channel groups measure at respective theoretical m/z values of the quantifier and qualifier of both the internal standard and the analyte and the two other multiple reaction monitoring channel groups measure at respective m/z values shifted to higher and lower values by a predefined level; ii) (130) comparing, for at least two groups, at least two of the quantifier/qualifier ratios of the multiple reaction monitoring transitions of the internal standard with a reference value from a database (126) by using at least one processing device (120), wherein the comparison comprises determining a deviation between the quantifier/qualifier ratios and the reference value; iii) (132) determining from the analyte and the internal standard measured multiple reaction monitoring transitions a measurement result by using the processing device (120), if the deviation for at least one of the quantifier/qualifier ratios is within at least one predefined tolerance range, otherwise rejecting (136) the measured multiple reaction monitoring transitions.

IPC Classes  ?

• H01J 49/00 - Particle spectrometers or separator tubes

Abstract

A computer-implemented method, system and decision support system adapted to provide arterial venous blood gas values without the provision of an arterial oxygenation saturation value or arterial blood gas values. The method comprises the provision of arterial blood gas values from a subject, for which said subject, only venous blood gas values are provided, by providing a mathematical model adapted to convert said venous blood gas values with a provided predefined default arterial oxygenation value to output arterial blood gas values of said subject. The present invention thus provides a method for providing arterial blood gas values from a specific subject without the need of providing an arterial blood sample from a painful arterial blood draw or the need for an arterial oxygenation saturation value of the subject, thus reducing distress to said patient and a reduction of tasks to relevant health care personnel.

IPC Classes  ?

• A61B 5/145 - Measuring characteristics of blood in vivo, e.g. gas concentration, pH-value
• A61B 5/00 - Measuring for diagnostic purposes ; Identification of persons
• G16H 50/50 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for simulation or modelling of medical disorders
• G01N 33/49 - Physical analysis of biological material of liquid biological material blood

Abstract

The present invention concerns the field of re-usable immunosensors. In particular, it relates to a method for regenerating an immunosensor comprising at least one polypeptide attached to an electroconductive surface of said immunosensor, wherein the at least one polypeptide is capable of specifically binding an analyte to be detected by the immunosensor, said method comprising the step of applying to the immunosensor at a temperature selected from the temperature range from about 35°C to about 42°C a positive electrical potential of about 0.3 V on said electroconductive surface of the immunosensor for a time sufficient to allow regeneration. Moreover, it also relates to a regenerated immunosensor obtainable by the method of the present invention and a system and device comprising the immunosensor as described herein, wherein said device is capable of applying a positive electrical potential of about 0.3 V on said electro conductive surface of the immunosensor for a time sufficient to allow regeneration. The present invention also contemplates, in general, the use of a temperature selected from the temperature range from about 35°C to about 42°C and a positive electrical potential of about 0.3 V on an electro conductive surface of an immunosensor as described in any one of claims 1 to 13 for regeneration of said immunosensor.

IPC Classes  ?

• G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

The present invention relates to monoclonal antibodies and antigen binding fragments that specifically bind to α-1,6-core-fucosylated alpha-fetoprotein (AFP), which is the core component of AFP-L3. Thus, the antibodies and antigen binding fragments provided herein may also be referred to as AFP-L3 antibodies. Also provided are polynucleotides encoding the antibodies or antigen binding fragments of the invention, host cells expressing the antibodies and antigen binding fragments of the invention, methods for producing the antibodies and antigen binding fragments of the invention, and uses of the antibodies and antigen binding fragments of the invention. Also provided herein is a pretreatment agent facilitating the binding of the antibodies and antigen binding fragments of the invention to α-1,6-core-fucosylated AFP. The present invention further relates to kits comprising the antibodies and antigen binding fragments of the invention and optionally the pretreatment agent of the invention.

IPC Classes  ?

• C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Abstract

The present disclosure refers to a method for operating a sorter device (5) in an IVD laboratory system, the method comprising providing a sorter device (5) in an IVD laboratory system (1) provided with a system operation controller (3), the sorter device (5) having a plurality of sample container racks each provided with reception holes for receiving sample containers (4), a handling device configured to pick sample containers (4) from and place sample containers (4) in the reception holes of the sample container racks, a sorter device controller configured to control operation of the sorter device (5) and connectable to the system operation controller (3), and an output device functionally connected to the sorter device controller and configured to output at least one of audio data and video data. The sorter device (5) is configured in a pre-operation process, the configuring comprising: assigning the plurality of sample container racks to a plurality of processing sub-targets conducted by the IVD laboratory system (1) in operation, wherein a first sample container rack is assigned to a first processing sub-target and a second sample container rack is assigned to a second processing sub-target which is different from the first processing sub- target, and assigning to the first sample container rack a first threshold value indicative of a first threshold number of sample containers (4) in the first sample container rack, wherein the first threshold number of sample containers (4) is smaller than a maximum number of sample containers (4) receivable in the first sample container rack. The sorter device (5) is operated in an operation process, the operating comprising: placing sample containers (4) in the reception holes of the first sample container rack by the handling device, determining a first present number of sample containers (4) received in the first sample container rack by the sorter device controller, comparing the first present number of sample containers (4) to the first threshold value by the sorter device controller, and if the first present number of sample containers (4) is equal to or greater than the first threshold value, outputting a first warning data through the output device. Furthermore, an IVD laboratory system (1) is provided.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

samplecalibration,avsamplecalibration,avcalibration,av by using the processing device (130).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 30/06 - Preparation
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• G01N 1/38 - Diluting, dispersing or mixing samples
• G01N 30/72 - Mass spectrometers

Abstract

The present invention relates to a method for determining a lactone analyte in a sample, comprising (i) contacting said sample with a derivatization reagent comprising a nucleophilic reagent; (ii) determining a nucleophilic reagent-derivative of said lactone analyte obtained in step (i), and (iii) determining said lactone analyte based on the determination of the nucleophilic reagent-derivative of said lactone analyte in step (ii). The present invention also relates to methods uses, compounds, kits and devices related thereto.

IPC Classes  ?

• G01N 33/94 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics

Abstract

A method for autonomous teach-in of at least one target position of a supply device (120) of a slide imaging apparatus (110) is disclosed. The slide imaging apparatus (110) comprises at least one imaging device (126, 128) configured to generate an image of a sample mounted on a slide (140). The target position is a position on the imaging device (126, 128). The slide imaging apparatus (110) comprises at least one operating system (170) configured for controlling operation of the supply device (120). The method comprises the following steps i) (186) providing at least six pre-defined positions by using the operating system (170); ii) (188) driving the supply device (120) to the six pre-defined positions until colliding with the imaging device (126, 128) by using the operating system (170) and detecting collisions with the imaging device (126, 128); iii) (190) evaluating the detected collisions by using the operating system (170), thereby determining the target position.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• B25J 9/16 - Programme controls
• G05B 19/425 - Teaching successive positions by numerical control, i.e. commands being entered to control the positioning servo of the tool head or end effector
• B25J 13/08 - Controls for manipulators by means of sensing devices, e.g. viewing or touching devices
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system

Abstract

ƒpp cƒpp PP ≥ 1. In each adjustment step a signal adjustment function or a concentration adjustment function is determined and at least one target concentration value is assigned.

IPC Classes  ?

• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
• G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

The invention relates to a method for determining at least one container information (coi) about a laboratory sample container (1), wherein the method comprises the steps: a) acquiring an image (ibc) comprising a brightness and/or color information (bci) of a possible region (2) of the container (1), b) acquiring a map (md) comprising a depth information (di) of the region (2), and c) determining the container information (coi) by fusing the brightness and/or color information (bci) and the depth information (di).

IPC Classes  ?

• G06V 10/80 - Fusion, i.e. combining data from various sources at the sensor level, preprocessing level, feature extraction level or classification level

Abstract

The invention relates to novel HIV gp41 antigen compositions that are suitable for detecting antibodies against HIV in an isolated biological sample providing high specificity immunoassay results. It further relates to methods detecting HIV antibodies, use of novel HIV gp41 antigen compositions in immunoassays as well as to reagent kits comprising novel HIV gp41 antigen compositions.

IPC Classes  ?

• C07K 14/16 - HIV-1
• G01N 33/569 - Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

This application discloses electrochemical cells, nanopore devices, and associated buffer compositions useful for nanopore-based nucleic acid sequencing. Also disclosed are methods for using the electrochemical cells, devices, and compositions in nanopore-based nucleic acid sequencing methods, such as nanopore Sequencing-by-Expansion (Nano-SBX) and nanopore Sequencing-by-Synthesis (Nano-SBS) methods.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

A position tracking system (112) for tracking a relative position between at least two connected modules (114) is disclosed. The position tracking system (112) comprises: - at least one target (118) associated with a first one of the at least two connected modules (114), wherein the at least one target (118) is at least one of arrangeable on and arrangeable within the first one of the at least two connected modules (114), wherein the at least two connected module (114) are mechanically interacting entities and/or components, configured for allowing transport of objects from one module to the other module; - at least one position sensor (120) associated with a second one of the at least two connected modules (114), wherein the position sensor (120) is configured for generating at least one sensor signal according to a relative position between the at least one position sensor (120) and the at least one target (118), wherein the at least one position sensor (120) is at least one of arrangeable on and arrangeable within the second one of the at least two connected modules (114); - at least one processing unit (122) configured for tracking a relative position between the connected modules (114) from the at least one sensor signal in at least one plane (124); and - at least one further sensor (140) configured for generating a further sensor signal according to at least one further parameter, wherein the further sensor (140) is selected from the group consisting of: a temperature sensor (142) and/or a humidity sensor, wherein the processing unit (122) is further configured for considering the at least one further sensor signal when determining the relative position between the connected modules (114). Further disclosed are a monitoring system (110) for monitoring at least two connected modules (114), method for tracking a relative position between at least two connected modules (114) by using at least one position tracking system (112) and a method for monitoring at least two connected modules (114) by using at least one monitoring system (110).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system

Abstract

The present invention relates to in vitro methods for aiding in the detection of hepatocellular carcinoma (HCC) in a subject comprising determining the amount of one or more glycan structure at position N207 of haptoglobin (i.e. of the B-chain of haptoglobin having the sequence given in SEQ ID NO: 1) in a sample obtained from said subject. Also disclosed are a glycan structure as well as a glycopeptide comprising said glycan structure, both of great utility in the detection of HCC. Further, the present invention relates to the use of one or more glycan structure at position N207 or of a glycopeptide comprising N207 of haptoglobin in combination with AFP and/or PIVKA in the detection of HCC.

IPC Classes  ?

• G01N 33/574 - Immunoassay; Biospecific binding assay; Materials therefor for cancer

Abstract

A method of separating stereoisomers of N-phthaloyl-glutamic acid is described. The method comprises passing a solution comprising an L/D stereoisomer mixture of N-phthaloyl-glutamic acid through a chiral chromatography column by normal phase chromatography, wherein the chiral chromatography column comprises, as a stationary phase, silica having groups represented by formula (1) as described herein covalently bonded to a surface thereof.

IPC Classes  ?

• B01D 15/32 - Bonded phase chromatography, e.g. with normal bonded phase, reversed phase or hydrophobic interaction
• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• B01J 20/24 - Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
• B01J 20/29 - Chiral phases
• B01J 20/289 - Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
• B01J 20/285 - Porous sorbents based on polymers
• C12P 13/14 - Glutamic acid; Glutamine

Abstract

The present invention relates to in vitro methods for aiding in the detection of hepatocellular carcinoma (HCC) in a subject. The method may comprise determining the amount of one or more N-glycan structure attached to haptoglobin (i.e. of the β- chain of haptoglobin having the sequence given in SEQ ID NO: 1) in a sample obtained from said subject and comparing the amount of said one or more glycan structure to a reference amount of said one or more glycan structure, wherein an altered amount of said one or more glycan structure in said patient sample relative to the reference amount of said one or more glycan structure is indicative for HCC. Further, the present invention relates to the use of one or more glycan structure attached to haptoglobinor of a glycopeptide derived from haptoglobin in combination with AFP and/or PIVKA-II in the detection of HCC.

IPC Classes  ?

• G01N 33/574 - Immunoassay; Biospecific binding assay; Materials therefor for cancer

Abstract

Techniques described herein include, for example, generating a feature map for an input image, generating a plurality of concentric crops of the feature map, and generating an output vector that represents a characteristic of a structure depicted in a center region of the input image using the plurality of concentric crops. Generating the output vector may include, for example, aggregating sets of output features generated from the plurality of concentric crops, and several methods of aggregating are described. Applications to classification of a structure depicted in the center region of the input image are also described.

IPC Classes  ?

• G06V 10/44 - Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersections; Connectivity analysis, e.g. of connected components
• G06T 7/00 - Image analysis
• G06V 10/82 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks
• G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts

Abstract

A machine learning and/or deep learning framework was constructed and used to forecast COVID-19 cases and deaths. Multiple open data sources relevant for the pandemic evolution in a geographic area, such as the United States or another country or region, can be processed to extract a plurality of features, such as localized (i.e., county level, city level, regional level, province level, etc.) COVID cases and deaths, demographics and socioeconomic factors, non- medical interventions, and mobility (i.e., from cell phone and/or GPS data).

IPC Classes  ?

• G16H 50/80 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for detecting, monitoring or modelling epidemics or pandemics, e.g. flu

Abstract

The present invention relates to methods for assessing whether or not a patient has aggressive prostate cancer by determining the levels of particular glycoforms attached to prostate specific antigen (PSA) protein in a biofluid sample of a subject, and comparing the determined level or concentration to a reference. The methods are particularly useful for assessing subjects that have 2-10 ng/ml total PSA in the subject's serum.

IPC Classes  ?

• G01N 33/574 - Immunoassay; Biospecific binding assay; Materials therefor for cancer

Abstract

The present disclosure relates to a process for the regiochemically and enantiomerically controlled synthesis of phosphoramidite-containing monomers, and to intermediate products of this process. In some embodiments, the phosphoramidite-containing monomers or their precursors are regioisomerically and/or enantiomerically pure and may be polymerized into polymers or copolymers.

IPC Classes  ?

• C07F 9/24 - Esteramides
• C07F 9/6518 - Five-membered rings
• B01J 19/00 - Chemical, physical or physico-chemical processes in general; Their relevant apparatus
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Disclosed herein are techniques for facilitating a clinical decision for a patient based on identifying a group of patients having similar attributes as the patient. The group of patients can be identified using information from a predictive machine learning model that performs a clinical prediction for the patient. At least some of the attributes of the group of patients can be output to support a clinical decision. The attributes may include, for example, biography data of the patient, results of one or more laboratory tests of the patient, biopsy image data of the patient, molecular biomarkers of the patient, a tumor site of the patient, and a tumor stage of the patient.

IPC Classes  ?

• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients

Abstract

The present invention relates to a method for assessing myocardial infarction comprising the steps of determining the amount of a first biomarker in a sample of a subject, said first biomarker being a cardiac Troponin, determining the amount of a second biomarker in a sample of the subject, wherein said second biomarker is selected from the group consisting of: a BMP 10-type peptide (Bone Morphogenic Protein 10-type peptide), FGF23 (Fibroblast growth factor 23), a BNP -type peptide, cardiac myosin binding protein C (cMyBPC) and ANG2 (Angiopoietin 2), comparing the amounts of the biomarkers to references for said biomarkers and/or calculating a score for assessing myocardial infarction based on the amounts of the biomarkers, and assessing said subject based on the comparison and/or the calculation. The invention also relates to the use of a first biomarker being a cardiac Troponin and a second biomarker selected from the group consisting of: a BMP 10-type peptide (Bone Morphogenic Protein 10-type peptide), FGF23 (Fibroblast growth factor 23), a BNP -type peptide, cardiac myosin binding protein C (cMyBPC) and ANG2 (Angiopoietin 2), or at least one detection agent for said first biomarker and at least one detection agent for said second biomarker for assessing myocardial infarction. Moreover, the invention further relates to a computer-implemented method for assessing myocardial infarction and a device and a kit for assessing myocardial infarction.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Abstract

The present invention relates to a method for assessing myocardial infarction comprising the steps of determining the amount of a first biomarker in a sample of a subject, said first biomarker being cMyBPC, determining the amount of a second biomarker in a sample of the subject, wherein said second biomarker is selected from the group consisting of: a BMP10- type peptide (Bone Morphogenic Protein 10-type peptide), FGF23 (Fibroblast growth factor 23), a BNP-type peptide (Brain natriuretic peptide type peptide), GDF-15 (Growth differentiation factor 15), ANG2 (Angiopoietin 2), CRP (C-reactive protein), ESM1 (endothelial cell specific molecule 1), or a lipid biomarker, such as Cholesterol, LDL (Low Density Lipoprotein) or APOAT (Apolipoprotein A-1) comparing the amounts of the biomarkers to references for said biomarkers and/or calculating a score for assessing myocardial infarction based on the amounts of the biomarkers, and assessing said subject based on the comparison and/or the calculation. The invention also relates to the use of a first biomarker being cMyBPC and a second biomarker selected from the group consisting of: a BMP10-type peptide, FGF23, a BNP-type peptide, GDF15, ANG2, CRP (C-reactive protein), ESM1, or a lipid biomarker, such as Cholesterol or LDL, or at least one detection agent for said first biomarker and at least one detection agent for said second biomarker for assessing myocardial infarction. Moreover, the invention further relates to a computer-implemented method for assessing myocardial infarction and a device and a kit for assessing myocardial infarction.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
• G01N 33/92 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol

Abstract

The present invention includes: a reagent vessel holding part that holds a reagent vessel for accommodating a reagent; a reagent vessel information detection unit that detects information relating to the reagent vessel; a display unit that displays a screen including an operation region where operations from a user are received; and a control unit that causes the display unit to display the screen. The control unit has: a vessel presence/absence determination unit that determines whether the reagent vessel is present in the reagent vessel holding unit on the basis of the information detected by the reagent vessel information detection unit; a first operation region output unit that outputs, to the screen, a first operation region which is operated when the user has confirmed removal of the reagent vessel; a second operation region output unit that outputs, to the screen, a second operation region which is operated when shutdown is performed; and a warning output unit that outputs, to the screen, a warning prompting removal of the reagent vessel when the vessel presence/absence determination unit has determined that the reagent vessel is present. The foregoing makes it possible to provide an automated analysis device which prevents shutdown from starting while a reagent has been left behind.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

The present invention relates to a method for determining at least one analyte of interest and the use thereof. The present invention further relates to a kit, a complex, a method to synthesize a complex, a monomer and the use thereof for detecting the analyte of interest in the sample.

IPC Classes  ?

• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

Abstract

The present invention concerns the field of diagnostics. Specifically, it relates to a method for assessing a subject with suspected infection comprising the steps of determining the amount of a first biomarker in a sample of the subject, said first biomarker being DLL1, determining the amount of a second biomarker in a sample of the subject, said second biomarker being GDF15, comparing the amounts of the biomarkers to references for said biomarkers and/or calculating a score for assessing the subject with suspected infection based on the amounts of the biomarkers, and assessing said subject based on the comparison and/or the calculation. The invention also relates to the use of a first biomarker being DLL1 and a second biomarker being GDF15, or a detection agent specifically binding to said first biomarker and a detection agent specifically binding to said second biomarker for assessing a subject with suspected infection. Moreover, the invention further relates to a computer-implemented method for assessing a subject with suspected infection and a device and a kit for assessing a subject with suspected infection.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

A method of cross-flow filtering wastewater from a diagnostic apparatus or a laboratory analyser, wherein the wastewater comprises nanoparticles and/or microparticles, and the wastewater is streaming in a laminar flow across a surface of a filter membrane, the method comprising: (a) streaming the wastewater across the surface of the filter membrane with a flow rate, so that the flow of the wastewater is a laminar flow with a Reynolds number (Re) of smaller than 500; (b) streaming the wastewater in pulse cycles across the surface of the filter membrane, wherein each pulse cycle comprises one active phase in which the wastewater is under a duty pressure and one inactive phase in which the wastewater is under an inactive pressure, wherein the inactive pressure is no more than 10% of the duty pressure and the active phases have a duration of greater than 50 % of the corresponding pulse cycles; and (c) separating the nanoparticles and/or microparticles from the wastewater when the wastewater passes through the filter membrane. Also described is a cross-flow filtration system configured for performing the method.

IPC Classes  ?

• B01D 61/12 - Controlling or regulating
• B01D 61/22 - Controlling or regulating
• B01D 65/08 - Prevention of membrane fouling or of concentration polarisation
• C02F 1/44 - Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis

Abstract

Provided is a component abnormality detecting system for detecting an abnormality of a component of a liquid transport system that sucks in and discharges a liquid to and from a sample inspection sensor of an automated analyzing device, the component abnormality detecting system comprising a storage device for storing data of an electrical signal output by the sensor, and a processing device for processing the data recorded in the storage device, wherein the processing device detects an abnormality of a component of the liquid transport system on the basis of the electrical signal.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

pRR0000p0000p0iiiii, a statistical parameter derived from a linear regression of the plurality of past eGFR values; and applying a machine-learning model to the input data to generate an output indicating the likelihood of kidney failure within the given amount of time Δt. Corresponding training methods and systems are also provided.

IPC Classes  ?

• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment

Abstract

A method for operating a laboratory system is disclosed, comprising providing a laboratory system having a plurality of sample containers (1) configured to contain a sample to be processed for at least one of pre-analysis and analysis in the laboratory system; a plurality of laboratory devices (2; 3; 4; 6) providing for a plurality of target devices each configured to handle one or more sample containers from the plurality of sample containers (1), the one or more sample containers being assigned for handling to the target device in operation of the laboratory system; and a control device (5) configured to at least control assignment of the plurality of sample containers (1) to the plurality of target devices; and assigning the plurality of sample containers (1) to the plurality of target devices in operation of the laboratory system. The assigning comprises: determining a target device workload state for each of the plurality of target devices, the target device workload state being in a range between a first range limit indicative of a first capacity for handling sample containers and a second range limit indicative a second capacity for handling sample containers, the second capacity being a higher capacity than the first capacity for handling sample containers, and determined according to a metric being proportional to a resource target device state indicative of a present number of sample containers assigned to the target device, and a power of an output flow of the target device, the output flow being indicative of output of sample containers per time by the target device; assigning the plurality of sample containers (1) to the plurality of target devices according to the target device workload states; and providing the plurality of sample containers (1) to the plurality of target devices for handling according to the assignment. Furthermore, a laboratory system is provided.

IPC Classes  ?

• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G05B 19/042 - Programme control other than numerical control, i.e. in sequence controllers or logic controllers using digital processors
• G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control (DNC), flexible manufacturing systems (FMS), integrated manufacturing systems (IMS), computer integrated manufacturing (CIM)
• G06Q 50/22 - Social work
• G16H 40/20 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the management or administration of healthcare resources or facilities, e.g. managing hospital staff or surgery rooms

Abstract

The present invention relates to a composition for determining at least one analyte of interest via Mass Spectrometry measurements and uses thereof, a kit and the use thereof, a method of determining the level of at least one analyte of interest in an obtained sample, a sampling tube for collecting a patient sample, an analyzer as well as the use of dialkyl sulfide as an additive in a formulation of β-lactam antibiotic analyte for preventing oxidation.

IPC Classes  ?

• G01N 33/94 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics

Abstract

The present invention relates to a method of determining the level of at least one analyte of interest, sampling tubes for collecting a patient sample, the use of nucleophilic derivatization reagents, an analyzer as well as kits.

IPC Classes  ?

• G01N 33/94 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics

Abstract

The invention relates to a laboratory apparatus (1), wherein the laboratory apparatus (1) comprises, - a housing (2), wherein the housing (2) comprises a through-opening (3), wherein the through-opening (3) is adapted for passing through it a transport device (4) for transporting laboratory sample containers (5) in and/or out of the housing (2), and - a cover (6), wherein the cover (6) comprises at least two ring segments (7a, 7b), wherein the ring segments (7a, 7b) are adjustable to each other between a distant adjustment (da) with at least one distance (Dla, Dlb) in between ends (7aE, 7bE) of the ring segments (7a, 7b) for arranging them around the passed through transport device (4) and a near adjustment (na) with less or no distance in between the ends (7aE, 7bE) of the ring segments (7a, 7b) for surrounding the passed through transport device (4), and wherein the cover (6) in the near adjustment (na) is adapted to cover a part (3f) of the through-opening (3) left free by the passed through transport device (4), - wherein the laboratory apparatus (1) is a pre-analytical, analytical, and/or post-analytical laboratory apparatus (1'), in particular a sorting module (1").

IPC Classes  ?

• B01L 9/00 - Supporting devices; Holding devices
• G01N 21/13 - Moving of cuvettes or solid samples to or from the investigating station

Abstract

The present invention relates to methods to improve detection of amplification products from Loop-mediated Amplification (LAMP) reactions by the use of nucleotide analogs that form duplex structures that nucleic acid polymerase enzymes do not recognize efficiently.

IPC Classes  ?

• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates

Abstract

A computer-implemented method of differentiating between lymphoid blast cells and myeloid blast cells comprises: receiving (S30) a digital image containing one or more blast cells; applying (S34) a parametric model classifier (412) to one or more portions of the digital image each containing a respective blast cell, the parametric model (416) configured to generate an output (S38) indicative of whether each blast cell is a lymphoid blast cell or a myeloid blast cell. Computer- implemented methods of training a parametric model (416) are also provided, as well as a clinical decision support system (400) relying on the computer-implemented method of classifying blast cells.

IPC Classes  ?

• G06V 10/25 - Determination of region of interest [ROI] or a volume of interest [VOI]
• G06V 10/20 - Image preprocessing
• G06V 10/82 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 30/40 - ICT specially adapted for the handling or processing of medical images for processing medical images, e.g. editing

Abstract

The present invention relates to a modified antibody comprising a heavy chain and a light chain, wherein the antibody is modified to include in one or more of its immunoglobulin polypeptide chains one or more first recognition site(s) for the transglutaminase from Kutzneria albida (KalbTG) or a functionally active variant thereof. The one or more first recognition site(s) are introduced at one or more selected position(s) within an antibody's heavy chain and/or an antibody's light chain. The invention further relates to one or more nucleic acid(s) encoding an immunoglobulin polypeptide chain including the one or more recognition site(s), a site-specifically conjugated antibody comprising the modified antibody and one or more labelling domain(s) covalently attached to one or more first recognition sites, a kit for producing the conjugated antibody, a method of specifically labelling the modified antibody by way of site-specific conjugation, the use of the modified antibody for producing a specifically site-specifically conjugated antibody, a method of detecting a target in a sample and the use of the site-specifically conjugated antibody in the detection of a target and/or in the diagnosis.

IPC Classes  ?

• A61K 47/65 - Peptidic linkers, binders or spacers, e.g. peptidic enzyme-labile linkers
• A61K 47/68 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
• C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
• C12N 9/10 - Transferases (2.)
• C12N 15/117 - Nucleic acids having immunomodulatory properties, e.g. containing CpG-motifs

Abstract

A method of determining the concentration of at least one analyte in a sample (112) is proposed. The method comprises: i. providing at least one sensor device (110), the sensor device (110) comprising - at least one field effect transistor (114) having at least one source electrode (116), at least one drain electrode (118) and at least one gate electrode (120), • - at least one sensing electrode (132) configured for being in contact with the sample (112), the sensing electrode (132) being at least one of electrically connected to the gate electrode (120) of the field effect transistor (114) or integrated into the gate electrode (120) of the field effect transistor (114), and • - at least one control device (146), the control device (146) being configured for applying operation parameters to the field effect transistor (114) and for monitoring at least one signal value with the field effect transistor (114); • ii. at least one parameter selection step comprising selecting a set of operation parameters of the field effect transistor (114) for at least one subsequent measurement step, the parameter selection step comprising performing a plurality of evaluation measurements with the field effect transistor (114) by using various sets of operation parameter candidates and by selecting the set of operation parameters in accordance with at least one optimization criterion monitored during the evaluation measurements; and • iii. at least one measurement step comprising detecting the concentration of the analyte by applying the set of operation parameters selected in step ii. to the field effect transistor (114) and by determining at least one signal value with the field effect transistor (114). Further, a sensor device (110) for determining the concentration of at least one analyte in a sample (112) is proposed.

IPC Classes  ?

• G01N 27/414 - Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS

Abstract

Automated methods for genotyping melting curves are described. Melting curves are processed and difference matrices are compiled based on the processed matrices. The difference matrices are used to compile a cluster matrix and the cluster matrix is filtered to determine clusters that are used for genotyping the melting curves.

IPC Classes  ?

• G16B 25/10 - Gene or protein expression profiling; Expression-ratio estimation or normalisation
• G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
• G16B 40/30 - Unsupervised data analysis
• C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism

Abstract

The invention relates to a laboratory sample container carrier handling apparatus (1) comprising a revolving device (2) and a guiding surface (S), wherein an entry segment (EP) of the guiding surface (S) is adapted to smoothly receive a laboratory sample container carrier (3). The invention further relates to a laboratory automation system (100) comprising such a laboratory sample container carrier handling apparatus (1) and to a use of such a laboratory sample container carrier handling apparatus (1) for handling a laboratory sample container carrier (3) in, in particular such, a laboratory automation system (100).

IPC Classes  ?

• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system

Abstract

The present invention relates to a monoclonal antibody or an antigen-binding fragment thereof specifically binding to Aß42 with advantageous features for Aß42 detection in vitro using immunoassays. Also provided is a polynucleotide or a set of polynucleotides encoding the same and a vector comprising said polynucleotide(s). Further provided is a host cell comprising the polynucleotide(s) and a corresponding production process using this host cell. Also provided herein are uses and methods employing the monoclonal antibody or an antigen-binding fragment thereof specifically binding to Aß42 as provided herein.

IPC Classes  ?

• C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
• A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia

Abstract

A method for characterization of a mass spectrometry instrument (100) comprising at least one mass analyzing cell (102, 104, 106) is proposed. The method comprising the steps of analyzing a sample (110) comprising at least one substance having a known molecular weight by means of the mass spectrometry instrument (100) so as to provide a mass spectrum (116, 118, 144, 146) of the sample (110), determining an outer envelope and an inner envelope of the mass spectrum (116, 118, 144, 146), calculating a squared difference between the outer envelope and the inner envelope, and determining a deviation of the calculated squared difference from a theoretical mass to charge ratio value of the substance.

IPC Classes  ?

• H01J 49/00 - Particle spectrometers or separator tubes

Abstract

A computer-implemented method for performing a clinical prediction is disclosed. The method comprises the following steps: i) (110) retrieving input data via at least one communication interface (164) of a processing device (166), wherein the input data comprises multiple different modalities of a patient; ii) (114) processing the input data by using the processing device (166), wherein the processing comprises generating embedding modality representations from the input data by using at least one trainable data embedder, wherein the processing comprises combining the embedding modality representations using at least one aggregation network thereby generating the clinical prediction, wherein the aggregation network comprises at least one attention layer and/or at least one transformer layer; and iii) (118) generating an output of the clinical prediction by using the processing device (166).

IPC Classes  ?

• G06N 3/04 - Architecture, e.g. interconnection topology
• G16H 30/00 - ICT specially adapted for the handling or processing of medical images
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients
• G16H 20/00 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance
• G06N 3/045 - Combinations of networks
• G16H 30/40 - ICT specially adapted for the handling or processing of medical images for processing medical images, e.g. editing

Abstract

Tb0TTT. The present invention thus provides an automated analysis device with which it is possible to reduce the consumption of consumables that occurs when a reagent is registered, in comparison with a conventional device.

IPC Classes  ?

• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

Embodiments of the present disclosure relate to test result level based analysis. Some embodiments of the present disclosure provide a computer-implemented method. The method comprises obtaining a plurality of test result levels corresponding to a plurality of medical indicators of a patient, each test result level indicating that a quantitative test result of a corresponding medical indicator falls within one of a plurality of predetermined quantitative ranges; obtaining a plurality of reference test result levels corresponding to the plurality of medical indicators associated with a reference case, each reference test result level indicating that a quantitative reference test result of a corresponding medical indicator falls within one of the plurality of predetermined quantitative ranges; and determining a similarity between a medical condition of the patient and a reference medical condition associated with the reference case at least based on the plurality of test result levels and the plurality of reference test result levels. Through the solution, by means of test result level based analysis, it can protect the patient's privacy and obtain an accurate interpretation for the patient.

IPC Classes  ?

• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients

Abstract

The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying positively charged tags from oligonucleotide probes, bound to target nucleic acids, are produced during PCR by the 5`-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique positively charged tags.

IPC Classes  ?

• C12Q 1/6823 - Release of bound markers
• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/6872 - Methods for sequencing involving mass spectrometry

Abstract

A machine learning model for traversing a decision tree, the machine learning model trained from a structured data set including a first set of key-value pairs and subject-specific criteria using the key-value pairs. The first set of key-value pairs is transformed into a second set of key-value pairs, which are projected to a subject-specific point within a multi-dimensional space. The decision tree includes decision and leaf nodes. Each leaf node is connected to a root node via a leaf-node-specific trajectory. Each decision node corresponds to a criterion using a value in the second set of key-value pairs. For each leaf node, a leaf-node-specific point within the multi-dimensional space is determined using the leaf-node-specific trajectory, and a similarity score is determined using the leaf-node-specific and subject-specific points. A subset of the leaf nodes is identified using the scores. State or protocol information for each leaf node in the subset is retrieved.

IPC Classes  ?

• G06N 5/022 - Knowledge engineering; Knowledge acquisition
• G06N 20/00 - Machine learning

Abstract

In a first aspect, the invention relates to a method for preparing a magnetic bead comprising at least one magnetic particle (M) and a silica coating, wherein the silica coating comprises at least two silica layers, the method comprising steps (a) to (d), wherein at least one of steps (b), (c) and (d) is/are done under sonication, wherein sonication is done with an amplitude (peak-to-peak) in the range of from 50 to 250 µm. A second aspect of the invention is related to a magnetic bead comprising (i) at least one magnetic particle (M) and (ii) a silica coating, wherein the silica coat-ing comprises at least two silica layers; wherein the magnetic bead is stable against 7.5 M hydrochlorid acid and has a metal (cation) leaching rate in 7.5 M hydrochloric acid in the range of from 0.1 to 10%, wherein the metal (cation) leaching is deter-mined according to a complex formation of Fe2+ with bathophenanthroline according to Reference Example 8.2. In a third aspect, the invention relates to a functionalized magnetic bead comprising at least one, magnetic bead according to the second aspect, wherein an outer silica layer is functionalized with at least one group selected from the group consisting of amino group, azide group, alkyne group, carboxyl group, thiol group, epoxy group, aryl group and alkyl group. A fourth aspect of the invention is directed to a process for functionalizing a magnetic bead according to the second aspect comprising at least one magnetic particle (M) and a silica coating, wherein the silica coating comprises at least two silica layers. A fifth aspect of the invention is related to a method for preparing magnetic Fe3O4 supra particles and a sixth aspect relates to the use of a magnetic bead according to the second aspect or of a functionalized according to the fourth aspect for immobilization of acid stable biocatalysts or for solid-phase organic synthesis using acid-stable linker.

IPC Classes  ?

• H01F 1/33 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of soft-magnetic materials metallic particles having oxide skin
• H01F 1/34 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of soft-magnetic materials non-metallic substances, e.g. ferrites
• H01F 1/00 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties

Abstract

The present invention relates to a method for determining an analyte of interest by frequency detection and the use thereof, a modified nanopore, an analyzing system, a kit and the uses thereof.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

A method for controlling a laboratory system or device comprising at least one pipettor with a pressure sensor, at least one humidity sensor, at least one temperature sensor, and a control unit. The control unit receiving a humidity value from the humidity sensor and a temperature value from the temperature sensor, comparing the pair of humidity and temperature values with a threshold database connected to the control unit, the threshold database comprising, for different pairs of humidity and temperature values, instructions to activate or deactivate an anti droplet control system of the pipettor, determining if the anti droplet system of the pipettor has to be activated or deactivated, activating or deactivating said anti droplet system of the pipettor.

IPC Classes  ?

• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

A laboratory system or device, comprising a reagent storage area (1) comprising at least two reagent drawers (2, 3) mounted slidably between an open position (O) and a closed position (C), each of the reagent drawers comprising receptacles for a plurality of reagent cartridges (RC1, RC2), and at least two locking mechanisms (4, 5) for respectively locking/unlocking the at least two reagent drawers (2, 3) when in the closed position (C), at least one robotic handler (6) and a control unit (7), wherein the reagent storage area (1) is subdivided in a loading zone (LZ), a storage zone (SZ) and a pipetting zone (PZ), and the reagent storage area (1) further comprises a handling zone (HZ).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

Described herein is a variant pol6 polymerase having improved thermostability. The disclosed polymerases each contain one or more substitutions relative to SEQ ID NO: 1 selected from the group consisting of G12W/Y/F, K114W/F/I, L117L/F/P, N194F/W/V, M232W/G/R, G313E/Q/L, A451F/W/Y, K490W/F/Y, Q565Y/I/V, Q590P/V/Y, and D681G/N/H. Additionally, the present specification discloses substitutions that can reduce stuttering in such polymerases, the substitutions selected from the group consisting of N298L, L538R, P542A, I570H/T/W/R/N/G, N574L, E633W/F, S636F, E639K, and K655G.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Abstract

vanA vanBvanAvanBvanAvanBvanB.

IPC Classes  ?

• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

The present disclosure pertains to a method for operating a laboratory automation system (1), the laboratory automation system (1) comprising a carrier (10) comprising a reception place (11) for receiving a sample container (12) configured to contain a sample to be analyzed by a laboratory device (13); a placement device (14) configured to pick and place the sample container (12); an imaging device (15); and a data processing device (16) comprising at least one processor (17) and a memory (18). The method comprises detecting an image of the reception place (11) by the imaging device (15); determining whether the reception place (11) is free for receiving the sample container (12) and the reception place (11) is configured to receive the sample container (12), by applying a machine learning algorithm for image analysis of the image of the reception place (11) in the data processing device (16); and placing the sample container (12) in the reception place (11) by the placement device (14) if the reception place (11) is determined as free and configured to receive the sample container (12). Further, a laboratory automation system (1) is disclosed.

IPC Classes  ?

• G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
• G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G06T 7/00 - Image analysis

Abstract

A laboratory system or device comprising a control unit for controlling operation of the system or device, and a storage unit connected to the control unit and containing instruction for each assay type of the control type to be used when running said assay type, and, for each control type, an allocation rule for the control type. The control unit receiving at least one assay order comprising instructions to run at least one assay type on the system or device, determining for the at least one assay type comprised in the at least one assay order, the control type to be used, and the corresponding allocation rule for said control type, scheduling an assay run comprising instructions to include the determined control type based on the determined allocation rule, and controlling the system or device to perform the scheduled assay run.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

A method for optimizing handlers operation in a laboratory system or device comprising at least two handlers movably arranged within the system or device in a shared area, and a control unit for controlling the operation of the at least two handlers comprising the following steps: defining for each of the handlers a task, receiving a first operation schedule, the operation schedule comprising a sequence of task to be performed by the handlers, starting operation of the handlers according to the received operation schedule, receiving a second operation schedule, creating an updated operation schedule, and continuing operation of the handlers according to the updated operation schedule.

IPC Classes  ?

• G16H 40/60 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices
• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis

Abstract

A method for increasing operational security of a laboratory system or device comprising: a reagent storage area (1) comprising at least two reagent drawers (2, 3) mounted slidably along a first axis Y between an open position (O) and a closed position (C) and arranged stacked with respect to a second axis Z perpendicular to the first axis Y, and having each a position sensor (P2, P3) for determining the closed position (C) of the respective reagent drawer (2, 3), at least one robotic handler head (4) movably arranged in the reagent storage area at least along the first axis Y and the second axis Z a control unit (5) configured to control the operation of the robotic handler head (4) and connected to the position sensors (P2, P3), the method comprising the steps of defining for each reagent drawer (2, 3) a first forbidden zone along the first axis Y (Y) and a second forbidden along the second axis Z (Z2, Z3), determining by means of the position sensors (P2, P3) if the reagent storage drawers (2, 3) are in the closed position (C), and control the operation of the handler head (4).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

A method for controlling an anti droplet system of a laboratory system or device comprising at least one air displacement pipettor with a pressure sensor and a control unit for controlling operation of the pipettor, the method comprising the following steps controlling the pipettor to be moved such that the pipettor tip is immerged in a fluid to be aspirated, aspirate a predetermined volume of fluid, move the pipettor such that the pipettor tip is emerged from the fluid, continuously monitoring the pressure above the fluid column in the pipettor tip and generating pressure curve over time, and determining if a pressure increase above the fluid column has been detected.

IPC Classes  ?

• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

The invention relates in a first aspect to an azadibenzocyclooctyne derivative according to formula (I) or a salt thereof having specific substituents at the benzo rings of the DIBAC structure and having specific substituents connected to the nitrogen atom of the DIBAC structure. A second aspect of the invention is directed to a conjugate of formula (II), wherein a substituent R6nn-Z-. A third aspect of the invention relates to a method for the modification of a target molecule, wherein a conjugate according to the second aspect is reacted with a target molecule comprising a 1,3-dipole group or a 1,3-(hetero)diene group. In a fourth aspect, the invention is directed to the use of the conjugate according to the second aspect for bioorthogonal labeling and/or modification of a target molecule. A fifth aspect of the invention relates to a modified target molecule comprising the reaction product of a conjugate according to the second aspect and a target molecule comprising a 1,3-dipole group or a 1,3-(hetero)diene group, obtained or obtainable from the method of the third aspect. In a sixth aspect, the invention is related to a kit comprising a modified target molecule according to the fifth aspect as detector reagent and a suitable capture reagent.

IPC Classes  ?

• C07D 225/08 - Heterocyclic compounds containing rings of more than seven members having one nitrogen atom as the only ring hetero atom condensed with carbocyclic rings or ring systems condensed with two six-membered rings
• A61K 47/50 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates

Abstract

The present invention provides a novel monoclonal antibody specifically binding to L-Thyroxine (T4) and compositions and kits comprising such antibodies. Furthermore, provided are polynucleotides encoding such monoclonal antibodies, host cells expressing said antibodies, methods of producing such antibodies and diagnostic methods using such monoclonal antibodies. The monoclonal antibody of the invention comprises a heavy chain variable domain (VH) comprising V or A in position 33; Y in position 50; W in position 52; I in position 98, G, A or V in position 99; Y in position 100; and I in position 100b; and a light chain variable domain (VL) comprising amino acids H or Y in position 28; N or K in position 29; W in position 32; G or A in position 91; Y, W or F in position 92; S or T in position 93;Y or F in position 95b; N, S, T or Q in position 95c; and H in position 96, wherein the positions of the amino acids in the VH and the VL are indicated according to the Kabat numbering scheme, respectively.

IPC Classes  ?

• C07K 16/26 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones
• G01N 33/53 - Immunoassay; Biospecific binding assay; Materials therefor
• G01N 33/78 - Thyroid gland hormones

Abstract

An instrument (110) for automatically dissecting a biological specimen (112) on a slide (114) is proposed. The instrument (110) comprises at least one imaging system (116). The imaging system (116) comprises at least one camera (118) configured for sequentially imaging at least one image of the slide (114) at a plurality of slide positions. The imaging system (116) com- prises a relay lens system (120) having a fixed focal length. The relay lens system (120) is configured for relaying an impinging light beam (122) from the slide (114) to the camera (118). The instrument (110) further comprises a movable xy-stage (150) configured for setting the slide (114) position. The instrument (110) further comprises at least one processing unit (162) configured for generating a full slide (114) image by stitching the sequentially imaged images of the slide (114). Further, a method for automatically dissecting a biological specimen (112) on a slide (114) is proposed.

IPC Classes  ?

• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• G02B 21/26 - Stages; Adjusting means therefor
• G02B 21/24 - Base structure

Abstract

Methods for the rapid detection of the presence or absence of Malaria parasites (including Plasmodium) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Additionally, the assay can be a multiplex assay, to amplify and detect a plurality of Plasmodium targets simultaneously, which offers advantages over singleplex assays. Furthermore, primers and probes targeting Malaria parasites (including Plasmodium) and kits are provided that are designed for the detection of Plasmodium, including, but not limited to, the Plasmodium species of P. falciparum, P. vivax, P. ovale, P. knowlesi, and P. malariae. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of Malaria parasites (including Plasmodium).

IPC Classes  ?

• C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Abstract

The present invention relates to monoclonal antibodies binding to the Receptor Binding Domain of the Spike protein of SARS-CoV-2 virus, nucleic acids encoding said antibody, host cells producing the same, compositions and kits comprising said antibodies, method of detecting SARS-CoV-2 virus in a sample comprising using said antibodies and methods of using said antibodies in immunoassays.

IPC Classes  ?

• C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• A61P 31/14 - Antivirals for RNA viruses

Abstract

For high sequencing throughput, circuitry can compress read data generated in real-time by a sequencing device. Various compression techniques can be used. A stream of raw data can be processed to generate raw read data stream. The raw read data stream may include sub-streams of data comprising a header data sub-stream, a basecall sub-stream, and a quality score sub-stream. The sub-streams can be extracted and compressed using separate threads, and the compressed data can be recombined. Sequence reads corresponding to different copies of the same nucleic acid molecule may be clustered and used to generate a consensus read. The number of sequence reads that are used to generate the consensus read can be limited to a threshold when a consensus read is substantially accurate. After the limit is reached, data from any new raw read data corresponding to the same nucleic acid molecule may be discarded.

IPC Classes  ?

• G16B 30/10 - Sequence alignment; Homology search
• C12Q 1/6809 - Methods for determination or identification of nucleic acids involving differential detection
• C12Q 1/6869 - Methods for sequencing
• G16B 30/20 - Sequence assembly

Abstract

The present invention concerns the field of diagnostics. Specifically, it relates to a method for assessing a subject with suspected infection comprising the steps of determining the amount of a first biomarker in a sample of the subject, said first biomarker being MR-proADM, determining the amount of a second biomarker in a sample of the subject, wherein said second biomarker is selected from the group consisting of: sFlt-1, GDF15 and ESM1, comparing the amounts of the biomarkers to references for said biomarkers and/or calculating a score for assessing the subject with suspected infection based on the amounts of the biomarkers, and assessing said subject based on the comparison and/or the calculation. The invention also relates to the use of a first biomarker being MR-proADM and a second biomarker selected from the group consisting of: sFlt-1, GDF15 and ESM1, or a detection agent specifically binding to said first biomarker and a detection agent specifically binding to said second biomarker for assessing a subject with suspected infection. Moreover, the invention further relates to a computer-implemented method for assessing a subject with suspected infection and a device and a kit for assessing a subject with suspected infection.

IPC Classes  ?

• C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients

Abstract

An analytical system (100, 100') comprising a mass spectrometer (60) and an ionization source (61) coupled to the mass spectrometer (60) is herein disclosed. The analytical system (100, 100') further comprises an analytical fluidic system (10, 10') connectable to the ionization source (61) via a downstream valve (20) for infusing samples into the mass spectrometer (60) via the ionization source (61), a downstream pump (40) fluidically connectable to the ionization source (61) via the downstream valve (20), where the downstream pump (40) is fluidically connected to a plurality of fluid containers comprising respective fluids (41, 42, 43, 44), the fluids comprising at least one concentrated composition (44) for calibrating the mass spectrometer (60) and at least one diluent (42, 43) for diluting the at least one concentrated composition (44). The analytical system(100, 100') further comprises a controller (90) configured to control the downstream pump (40) in order to obtain at least one diluted composition (45) by automatically mixing at least one concentrated composition (44) with at least one diluent (42, 43) with a predetermined dilution factor, to infuse the at least one diluted composition (45) into the ionization source (61), to obtain a mass spectrum (62) of the at least one diluted composition (45) and to execute a calibration (63) of the mass spectrometer (60) based on an assessment (64) of the mass spectrum (62). A respective automated analytical method comprising calibration of a mass spectrometer (60), like mass axis check and/or adjustment, with selected chemical compositions is herein also disclosed.

IPC Classes  ?

• H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
• G01N 30/40 - Flow patterns using back flushing
• G01N 1/38 - Diluting, dispersing or mixing samples

Abstract

The present invention relates to a composition comprising (i) cesium iodide, (ii) ethylamine and/or formic acid, and (iii) methanol and/or water. The present invention further relates to a method for calibrating a mass spectrometry (MS) device comprising (I) determining a mass spectrum of a composition as specified; and (II) calibrating the MS device based on the mass spectrum determined in step (I). The present invention further relates to devices, kits, uses, and methods related thereto.

IPC Classes  ?

• H01J 49/00 - Particle spectrometers or separator tubes

Abstract

A centering holder (112) for centering and holding one sample tube (114) the centering holder (112) being adaptable to sample tubes (114) having different diameters is disclosed. The centering holder (112) comprises: at least two coupled centering fingers (122) arranged and configured to adapt to the diameter of the sample tube (112) and for applying a centering force on-to the sample tube (114) in a direction towards a center axis (124) of the centering holder (112), wherein each of the centering fingers (122) comprises at least one rod element (126) and at least one sleeve (128), wherein the sleeve (128 at least partially surrounds the rod element (126), wherein the sleeve (128) is configured for physically contacting the sample tube (114) thereby transferring the centering force onto the sample tube (114); and at least one base element (132) configured for supporting the at least two rod elements (126) of the centering fingers (122). Further, a sample handling system (110) is disclosed.

IPC Classes  ?

• B01L 9/06 - Test-tube stands; Test-tube holders
• B01L 9/00 - Supporting devices; Holding devices

Abstract

The present invention relates to a method for determining at least one analyte of interest. The present invention further relates to a sample element, an inlet, a composition, a kit and the use thereof for determining at least one analyte of interest.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
• G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

In some embodiments, methods, systems, software and uses are provided for synchronizing medical images displays. An input identifying a region of interest in a first medical image is received. A second region of interest in a second medical image is determined based the first region. The first medical image and a first indication of the first region are displayed in a first viewport of a GUI. The second medical image and a second indication of the second region are displayed in a second viewport of the GUI. A display adjustment input is received to adjust the displaying of one of the first or second region of interest. Based on the display adjustment input and the correspondence information, an adjustment of the displaying of the first region of interest in the first viewport and an adjustment of the displaying of the second region of interest in the second viewport is implemented.

IPC Classes  ?

• G16H 30/20 - ICT specially adapted for the handling or processing of medical images for handling medical images, e.g. DICOM, HL7 or PACS
• G06N 3/08 - Learning methods
• G06T 7/00 - Image analysis
• G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems

Abstract

A computer implemented method for automated quality check of chromatographic and/or mass spectral data is disclosed. The method comprises the following steps: a) (110) providing processed chromatographic and/or mass spectral data obtained by at least one mass spectrometry device (112); b) (114) classifying quality of the chromatographic and/or mass spectral data by applying at least one trained machine learning model on the chromatographic and/or mass spectral data, wherein the trained machine learning model uses at least one regression model (116), wherein the trained machine learning model is trained on at least one training dataset comprising historical and/or semi- synthetic chromatographic and/or mass spectral data, wherein the trained machine learning model is an analyte-specific trained machine learning model.

IPC Classes  ?

• G01N 30/86 - Signal analysis

Abstract

The present invention is provided with: a first mechanism group 202 for use only in analysis of biochemical items; a second mechanism group 203 for use only in analysis of immunological items; a shared mechanism group 204 for use in analysis of both biochemical items and immunological items; and a control unit 116 that operates the second mechanism group 203 and the shared mechanism group 204 without operating the first mechanism groups 202 when analysis of biochemical items is not required, and operates the first mechanism group 202 and the shared mechanism group 204 without operating the second mechanism group 203 when analysis of immunological items is not required. Accordingly, provided are: an automatic analysis device capable of reducing waiting time of a user and running cost as compared to conventional products; and a method for operating the automatic analysis device.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

The present invention comprises: a storage part for storing, in advance, a specimen container 103 in which a specimen is accommodated before measurement; a dispensing mechanism 4 for suctioning the specimen from the specimen container; an input unit for accepting a command to store an emergency specimen in the storage part; a control unit 7 for, after completion of a first action performed by the dispensing mechanism at the time at which the command is received, performing control so as not to start a prescribed second action subsequently performed by the dispensing mechanism; and a display unit for prompting a user to store the emergency specimen in the storage part. The storage part is provided with a first storage section for storing a normal specimen, and a second storage section for storing the emergency specimen. The control unit, after having performed control so that the second action is not started, then performs control such that the second storage section moves to a position at which the emergency specimen is to be inserted by the user. This makes it possible for an operation such as additional installation of a container to be carried out by an operator during an analysis action using an automatic analysis device comprising a disc-type container installation mechanism.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations