According to the present disclosure, there is provided a method for producing oligodendrocytes and progenitor cells thereof, comprising introducing inducers including a combination of OLIG and SOX or a combination of OLIG and NKX into iPS or ES cells. According to the present disclosure, there is also provided a method for producing oligodendrocytes and progenitor cells thereof, comprising introducing inducers including OLIG, SOX, ASCL, and NKX into somatic cells.
A valve including a case 3, a tube 1 disposed in the case 3, a hinge fulcrum 4 disposed in the case 3, a contact member 5 connected to the hinge fulcrum 4 and capable of coming into contact with the tube 1, and a driving device 10 configured to apply a non-contact force to the contact member 5 from outside of the case 3 and change a contact force that is applied to the tube 1 by the contact member 5 to change an inner diameter of the tube 1.
The present invention provides a culturing system and method for culturing cells that can culture cells in parallel. The culturing system comprises a cell storage 10 configured to store cells, a plurality of culture apparatuses 20, and a distribution flow path 30 configured to distribute the cells from the cell storage 10 to the culture apparatuses 20, in which each of the culture apparatuses 20 comprises a plurality of culture vessels 21, a supply flow path 22 configured to selectively supply a fluid from the distribution flow path 30 to the culture vessels 21, and a connection flow path 23 configured to connect the culture vessels 21; the culture vessels 21 are connected in series with the connection flow path 23 in each of the culture apparatuses 20; and the supply flow path 22 is configured to selectively supply the fluid to the culture vessels so that the cells are sequentially transferred among the culture vessels 21 through the connection flow path 23 in each of the culture apparatuses 20.
A method for producing nerve cells includes introducing NGN1 as a differentiation factor into stem cells. A method for producing nerve cells includes introducing only NeuroD4 as a differentiation factor into stem cells. A method for producing motor neurons includes introducing NGN2, ISL2, and LHX4 as differentiation factors into stem cells. A method for producing motor neurons includes introducing NGN1, ISL2, and LHX4 as differentiation factors into stem cells. A method for producing motor neurons includes introducing NeuroD4, ISL2, and LHX4 as differentiation factors into stem cells. A method for screening neurological disease therapeutic drugs includes introducing a differentiation factor into pluripotent stem cells derived from neurological disease patients and differentiating the cells into neural cells, applying a candidate drug to cells during differentiation of the pluripotent stem cells into the neural cells or to the differentiated neural cells, and screening the candidate drug on the basis of on the neural cells.
According to the present disclosure, there is provided a method for producing stem cells including: preparing somatic cells; preparing a chimeric virus including a virus-derived genomic RNA harboring an inducer RNA that induces somatic cells into stem cells and a virus-derived envelope surrounding the genomic RNA, wherein the genomic RNA and the envelope are derived from different viruses; and introducing the inducer RNA into the somatic cells using the chimeric virus.
A pump is provided that includes a tube 1, tube rotors 21A, 21B, and 21C in contact with the tube 1, a case 3 containing the tube 1 and tube rotors 21A, 21B, and 21C, and a drive apparatus 10 that rotates the tube rotors 21A, 21B, and 21C from outside the case 3 without contacting the tube rotors 21A, 21B, and 21C.
According to the present disclosure, there is provided a method for culturing factor-introduced cells, the method including culturing factor-introduced cells and recovering the factor-introduced cells and seeding at least part of the recovered cells in a medium for seeding. In addition, there is provided a method for culturing factor-introduced cells, the method including culturing factor-introduced cells and inducing the factor-introduced cells to somatic cells different from pluripotent stem cells without passaging.
An solution transfer device comprises a pump 60 and a substrate 70. The pump 60 comprises a tube 1 for transferring a solution; tube rotors 21A, 21B, 21C, which contact the tube 1; and a driver 10 for transferring a solution within the tube 1 by rotating the tube rotors 21A, 21B, 21C without contacting the tube rotors 21A, 21B, 21C. The substrate 70 is provided with a solution-transferring flow path that is connected to the tube 1 of the pump 60.
F04C 5/00 - Rotary-piston machines or pumps with the working-chamber walls at least partly resiliently deformable
F04B 43/00 - Machines, pumps, or pumping installations having flexible working members
C12N 1/00 - Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
9.
METHOD FOR MANUFACTURING INDUCED PLURIPOTENT STEM CELLS
According to the present disclosure, provided is a method for manufacturing induced pluripotent stem cells including preparing cells and introducing RNA into the cells, wherein the RNA includes RNA encoding a reprogramming factor and wherein, in the RNA introduced into the cells, double-stranded RNA is substantially removed.
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
10.
METHOD FOR MANUFACTURING INDUCED PLURIPOTENT STEM CELLS
According to the present disclosure, there is provided a method for manufacturing pluripotent stem cells including introducing a reprogramming factor into cells and seeding the cells, into which the reprogramming factor is introduced, at a low concentration and culturing the cells.
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
This cell production device comprises: a cell production plate that includes a fluid circuit in which a plurality of functional sites are integrated; and a closed type connector that connects the fluid circuit to an external space in a closed manner, wherein the fluid circuit comprises, as the plurality of functional sites, an injection and discharge unit that injects or discharges a fluid into or out of the fluid circuit via the closed type connector, a variable volume unit that stores a fluid that is extruded or withdrawn by the injected or discharged fluid, and a cell induction and culture unit that performs at least one of induction and culture of cells based on the injected fluid.
This cell production device comprises: a cell production plate that includes a fluid circuit having a plurality of functional parts integrated therein; and a plurality of closed type connectors that connect the fluid circuit to an external space in a closed manner. The fluid circuit comprises, as the plurality of functional parts: a plurality of injection and discharge units capable of injecting or discharging a plurality of types of fluids into or out of the fluid circuit via the plurality of closed type connectors; a plurality of fluid reservoir units capable of storing the plurality of types of fluids to be injected or discharged; and a cell induction and culture unit that performs at least one of induction and culture of cells based on the stored plurality of types of fluids.
This cell treatment device is provided with: a factor introduction device 30 for introducing a pluripotent induction factor into cells so as to prepare induction factor-introduced cells; and a reprogramming suspension culture vessel for culturing the induction factor-introduced cells that have been prepared by the factor introduction device 30.
A microscope observation system comprises a cell production device provided with a culture vessel in which cells or cell masses are cultured, a microscope device capable of observing an object including cells or cell masses, a conveying apparatus that conveys the microscope device to the cell production device, and an operating control section that sends a command to the conveying apparatus to temporarily move the conveying apparatus from a current position to a relay position and then to move the conveying apparatus to a target position when focusing the microscope device onto the object, wherein a first distance from the current position to the relay position is a distance necessary to drive an actuator by at least a predetermined amount in order to actuate a rotation shaft or a translation shaft of the conveying apparatus, and a second distance from the current position to the target position is a shorter distance than the first distance.
A somatic cell production system comprising a preintroduction cell solution-feeding channel 20 through which a preintroduction cell-containing solution passes, a factor introducing device 30 that is connected to the preintroduction cell solution-feeding channel 20 and introduces a somatic cell inducing factor into preintroduction cells to prepare inducing factor-introduced cells, and a cell preparation device 40 in which the inducing factor-introduced cells are cultured to prepare somatic cells.
A cell culture device (200) includes a cell culture vessel (22) for culturing cells, a factor container (81) for storing a factor, a reagent container (82) for storing a reagent for introducing the factor into the cells, and a flow path for transporting the factor and the reagent from the factor container (81) and the reagent container (82) to the cell culture vessel (22).
A stem cell production system provided with a preintroduction cell-feeding solution channel 20 through which a solution containing cells passes, an induction factor-feeding solution mechanism 21 for feeding a pluripotency induction factor to the preintroduction cell-feeding solution channel 20, a factor introduction device 30 connected to the preintroduction cell-feeding solution channel 20 for making cells with induction factor introduced by introducing the pluripotency induction factor into the cells, a cell mass-making device 40 for making multiple cell masses comprising stem cells by culturing the cells with induction factor introduced, and a packaging device 100 for sequentially packaging each of the multiple cell masses.
This cell production device comprises: a cell production plate with a first side and a second side, comprising a cell induction and culture tank configured to perform at least one of induction and culture of cells, and a culture medium reservoir tank configured to store a culture medium to be supplied to the cell induction and culture tank; a warming element that is arranged at or near the first side of the cell production plate and that warms the cell induction and culture tank; and a cooling element that is arranged at or near the second side of the cell production plate and that cools the culture medium reservoir tank.
A cell processing system comprising an enclosure 601, an outer enclosure 701 that envelops the enclosure 601, an intake air purification filter 602 provided in the enclosure 601, that purifies gas that has been drawn in from outside the enclosure 601, a circulating apparatus, inside the outer enclosure 701, that circulates gas inside and outside the enclosure 601 in such a manner that gas in the outer enclosure 701 is drawn into the enclosure 601 through the intake air purification filter 602 and gas inside the enclosure 601 is discharged into the outer enclosure 701, and a cell processing apparatus for processing of cells, disposed inside the enclosure 601.
Provided is a cell culture method including introducing a factor into cells in a cell culture vessel, and culturing the cells into which the factor has been introduced in the same cell culture vessel. Also provided is a mononuclear cell collection method including treating blood to prepare a treated blood from which erythrocytes have been at least partially removed, diluting the treated blood, causing sedimentation of mononuclear cells contained in the diluted treated blood, removing the supernatant from the diluted treated blood, and collecting the mononuclear cells.
Provided is an erythrocyte removal device 100 including a blood container 10 that holds blood and an erythrocyte removal vessel 11 that at least partially removes erythrocytes from blood.
There is provided a cell culture vessel for culturing a cell in an interior thereof, wherein a width of a bottom surface is equal to or larger than a height of a side surface, the cell culture vessel comprising a flow path configured to supply a fluid into the interior, and wherein the interior is able to be closed.
According to the present disclosure, there is provided a method for culturing cells into which a reprogramming factor is introduced including culturing cells into which a reprogramming factor is introduced; and recovering all cells into which the reprogramming factor is introduced and seeding and passaging at least part of the recovered cells in a medium. In addition, there is provided a method for culturing cells into which a reprogramming factor is introduced, including culturing cells into which a reprogramming factor is introduced; and inducing somatic cells different from pluripotent stem cells without passaging the cells into which the reprogramming factor is introduced.
A cell culture vessel comprising a housing in which a culture chamber is provided. In the housing, at least two holes that connect the outside of the housing and a culture chamber are provided.
This cell production system is provided with: a robot that assists cell production; and a plurality of closed system cell production devices which are affected by the robot in a one-to-many manner, wherein the closed system cell production devices are each provided with a double-sided structure of a dangerous region side affected by the robot and a safe region side on the reverse side of the dangerous region side.
A method for producing a cardiomyocyte including preparing a stem cell, introducing a Sendai virus into the stem cell by infection, expressing mRNA for synthesizing an inducing factor from the Sendai viruses in the stem cell to induce a cardiomyocyte from the stem cell.
The present invention provides a stem cell manufacturing system comprising: a pre-transfer cell solution sending channel 20 through which a solution containing cells flows; an inducer solution sending mechanism 21 which sends a pluripotency inducer into the pre-transfer cell solution sending channel 20; an inducer transfer apparatus 30 which is connected to the pre-transfer cell solution sending channel 20 and transfers the pluripotency inducer into the cells to produce cells harboring the inducer; a cell cluster production apparatus 40 which cultures the cells harboring the inducer to produce a plurality of cell clusters consisting of stem cells; and a packaging apparatus 100 which sequentially packages the plurality of cell clusters.
Provided are a pharmaceutical composition or a pharmaceutical composition raw material, which contains supernatant from a culture medium used during culture to induce pluripotent stem cells.
A61K 8/98 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution of animal origin
A61P 17/14 - Drugs for dermatological disorders for baldness or alopecia
Provided is a pharmaceutical composition including a supernatant of a culture medium that has been used for maintenance culture of stem cells in an undifferentiated state. The stem cells for the pharmaceutical composition may be pluripotent stem cells.
A61K 8/98 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution of animal origin
A system for an iPS cell bank includes a terminal and a server. The terminal receives and sends a production request including a desired collection date of a somatic cell and a customer ID to the server. The server stores a collectable date for collecting the somatic cell, a producible period for producing an iPS cell, and a location and a stockable period for stocking the iPS cell; and determines: a collection date based on the desired collection date and the collectable date; a production period based on the collection date and the producible period; an acceptance date of the somatic cell based on the collection date and the production period; a stock location and a stock period based on the production period, the stockable location, and the stockable period; and a shipment date of the iPS cell based on the production period, the stockable location, and the stockable period.
A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/02 - Apparatus for enzymology or microbiology with heat exchange means
G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
G16B 25/00 - ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
C12M 1/00 - Apparatus for enzymology or microbiology
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
G16B 25/10 - Gene or protein expression profiling; Expression-ratio estimation or normalisation
35.
Stem cell manufacturing system, stem cell information management system, cell transport apparatus, and stem cell frozen storage apparatus
A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.
G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
G16B 25/00 - ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
C12M 1/00 - Apparatus for enzymology or microbiology
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
G16B 25/10 - Gene or protein expression profiling; Expression-ratio estimation or normalisation
A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/02 - Apparatus for enzymology or microbiology with heat exchange means
G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
G16B 25/00 - ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
G16B 25/10 - Gene or protein expression profiling; Expression-ratio estimation or normalisation
A first terminal determines a collection date of a somatic cell based on a desired collection date and a collectable date transmitted by a second medium; and outputs the collection date to a first medium. A second terminal outputs the collectable date to the second medium; and determines an acceptance date based on the collection date transmitted by the first medium and a production period of an iPS cell transmitted by a third medium. A third terminal determines the production period based on the collection date and a producible period, and determines a shipment date based on the production period and stockable location and period transmitted by a fourth medium; and outputs the production period to the third medium. A fourth terminal outputs the stockable location and period to the fourth medium; and determines stock location and period based on the production period and the stockable location and period.
G06Q 10/06 - Resources, workflows, human or project management; Enterprise or organisation planning; Enterprise or organisation modelling
G16H 40/20 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the management or administration of healthcare resources or facilities, e.g. managing hospital staff or surgery rooms
38.
Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
The present invention provides a stem cell manufacturing system comprising: a sending channel (20) through which a solution containing cells flows; an apparatus (30) which is connected to the sending channel (20) and transfers a pluripotency inducer into the cells to produce cells harboring the inducer; and an apparatus (40) which cultures the cells harboring the inducer to produce cell clusters consisting of stem cells.
A stem cell production system provided with a preintroduction cell-feeding solution channel 20 through which a solution containing cells passes, an induction factor-feeding solution mechanism 21 for feeding a pluripotency induction factor to the preintroduction cell-feeding solution channel 20, a factor introduction device 30 connected to the preintroduction cell-feeding solution channel 20 for making cells with induction factor introduced by introducing the pluripotency induction factor into the cells, a cell mass-making device 40 for making multiple cell masses comprising stem cells by culturing the cells with induction factor introduced, and a packaging device 100 for sequentially packaging each of the multiple cell masses.
A system for an iPS cell bank includes a terminal and a server. The terminal receives and sends a production request including a desired collection date of a somatic cell and a customer ID to the server. The server stores a collectable date for collecting the somatic cell, a producible period for producing an iPS cell, and a location and a stockable period for stocking the iPS cell; and determines: a collection date based on the desired collection date and the collectable date; a production period based on the collection date and the producible period; an acceptance date of the somatic cell based on the collection date and the production period; a stock location and a stock period based on the production period, the stockable location, and the stockable period; and a shipment date of the iPS cell based on the production period, the stockable location, and the stockable period.
A first terminal determines a collection date of a somatic cell based on a desired collection date and a collectable date transmitted by a second medium; and outputs the collection date to a first medium. A second terminal outputs the collectable date to the second medium; and determines an acceptance date based on the collection date transmitted by the first medium and a production period of an iPS cell transmitted by a third medium. A third terminal determines the production period based on the collection date and a producible period, and determines a shipment date based on the production period and stockable location and period transmitted by a fourth medium; and outputs the production period to the third medium. A fourth terminal outputs the stockable location and period to the fourth medium; and determines stock location and period based on the production period and the stockable location and period.
G16H 40/20 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the management or administration of healthcare resources or facilities, e.g. managing hospital staff or surgery rooms
42.
Stem cell manufacturing system, stem cell information management system, cell transport apparatus, and stem cell frozen storage apparatus
A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.
G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
G16B 25/00 - ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
G16B 25/10 - Gene or protein expression profiling; Expression-ratio estimation or normalisation
patents
