A device for heating a sample according to the present disclosure includes: a thermal block unit accommodating a reaction vessel; a heat transfer module thermally connected to the thermal block unit; and a heat sink thermally connected to the heat transfer module, wherein the thermal block unit includes: a thermal block having a plurality of accommodating portions for accommodating the reaction vessel; and a heating plate having a plurality of holes into which the plurality of accommodating portions are inserted.
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
H05B 3/34 - Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater flexible, e.g. heating nets or webs
2.
COMPUTER-IMPLEMENTED METHOD FOR PREPARING OLIGONUCLEOTIDES USED TO DETECT NUCLEOTIDE MUTATION OF INTEREST
The present invention relates to a computer-implemented method for preparing oligonucleotides used to detect a nucleotide mutation of interest in a target nucleic acid sequence. The present invention can provide oligonucleotides capable of detecting a nucleotide mutation on the basis of an integrated design rule, without the need to develop detailed design rules and modules considering the type of nucleotide mutation, the size of the mutation, whether a target to be detected is a wild-type and/or mutant sequence, sequence contents, and the like, by inputting information about a wild-type target nucleic acid sequence and a nucleotide mutation of interest, providing wild-type and mutant target nucleic acid sequences through the use of the input information, designing oligonucleotides for the mutant target nucleic acid sequence, and analyzing the matching between the designed oligonucleotides and the wild-type target nucleic acid sequence to select and provide oligonucleotides satisfying predetermined selection criteria.
According to an embodiment, a mobile diagnostic structure comprises a housing including a space therein; a partitioning module partitioning the space to include a preparation room and an analysis room; an inlet module providing an incoming path from an outside to the preparation room for a raw sample; and a transfer module providing a transfer path from the preparation room to the analysis room, for a pre-treated sample which is a result of pre-treating the raw sample in the preparation room.
E04H 3/08 - Hospitals, infirmaries, or the like; Schools; Prisons
B60P 3/14 - Vehicles adapted to transport, to carry or to comprise special loads or objects the object being a workshop for servicing, for maintenance, or for carrying workmen during work
4.
COMPUTER-IMPLEMENTED METHOD FOR PROVIDING NUCLEIC ACID SEQUENCE DATA SET FOR DESIGN OF OLIGONUCLEOTIDE
The present invention relates to a computer-implemented method for providing a nucleic acid sequence data set for the design of an oligonucleotide used to detect a target nucleic acid molecule of an organism of interest. In the present invention, nucleic acid sequence data retrieved by synonyms for a target nucleic acid molecule are sorted according to the taxonomic name and/or taxonomic identification (ID); taxonomic representative sequences are selected among nucleic acid sequence data having the same taxonomic name and/or taxonomic ID; and the selected taxonomic representative sequences are grouped according to the homology to select a group representative sequence in each group; and then nucleic acid sequence data having a homology of a predetermined value or more with the group representative sequence are provided.
G16B 25/20 - Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
G16B 5/00 - ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
G16B 20/20 - Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
Disclosed herein is a method for a positive control reaction using a pre-positive control composition. Unlike conventional positive controls, the pre-positive control composition according to the present disclosure is provided in the pre-positive control composition form, but not in a complete positive control form, until an experiment preparation stage, and can produce a complete-positive control through a positive control reaction, whereby the contamination that could occur in an experiment preparation stage for positive control preparation and positive control reactions can be minimize and an examination can be made to see whether the contamination comes from the positive control or not.
A cartridge for detecting a target analyte according to an embodiment of the present disclosure comprises a sample containing part in which a sample is receivable; a sample drawing part in which the sample is drawn up into a pipette tip; and a sample flowing channel via which the sample containing part and the sample drawing part are in fluid communication with each other, wherein a sample flows into a pipette tip from the sample containing part via the sample flowing channel by a negative pressure delivered through the pipette tip entering the sample drawing part in a state where the pipette tip is in close contact with an outlet of the sample flowing channel.
Provided a thermal cycler including a thermal block housing comprising a thermal block accommodating a reaction vessel and a temperature control portion for controlling a temperature of the thermal block, a support frame provided at the lower side of the thermal block housing, and at least one damping module comprising a fastening member coupled to the thermal block housing and the support frame and an elastic member provided between the thermal block housing and the support frame and elastically supporting the thermal block housing while spaced apart from the support frame.
The present invention relates to novel modules for transferring magnetic beads, an automated system comprising the same and a method for extracting nucleic acids using the same. The specifically designed magnet module and cover module of the present invention can be employed in the automated liquid handling apparatus by means of pre-existing moving modules (e.g., pipettor module) of the apparatus. The present invention enables a bead transfer-type method for extracting nucleic acids to be performed in an automated manner on the automated liquid handling apparatus. The present invention provides advantages of higher level of automation, more reduced cost and no need for another separate liquid handling apparatus compared to the conventional bead transfer-type method usually performed in the small apparatus designed to be used only for this bead transfer-type method. Also, the present method has the merits of more shortened reaction time compared to the conventional liquid transfer-type method.
Disclosed herein is a method for providing a preparation for detecting a target nucleic acid sequence in a specimen. According to an embodiment, conventional nucleic acid extraction processes performed in many steps can be omitted, whereby the shortage of nucleic acid extraction reagents can be solved and a preparation for detecting target nucleic acid sequence in a specimen can be supplied in an inexpensive and simple manner.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
10.
SAMPLING KITS AND METHODS FOR DETECTING RESPIRATORY PATHOGENS
The present invention relates to sampling kits and methods for detecting respiratory pathogens, and has advantages of showing a positive rate equivalent to that in a conventional detection method using nasopharyngeal swabs; and allowing self-sampling.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
A61B 10/00 - Other methods or instruments for diagnosis, e.g. for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
A61B 10/02 - Instruments for taking cell samples or for biopsy
11.
COMPUTER-IMPLEMENTED METHOD FOR PROVIDING COVERAGE OF OLIGONUCLEOTIDE SET FOR PLURALITY OF NUCLEIC ACID SEQUENCES
The present invention relates to a computer-implemented method for providing a coverage of an oligonucleotide set for a plurality of nucleic acids. The present invention provides nucleic acid sequences with the generation of probe-hybridized amplicons and/or nucleic acid sequences without the generation of probe-hybridized amplicons, by a combination of oligonucleotides according to match or mismatch information and position information of a forward primer, a probe, and a reverse primer included in an oligonucleotide set, and thus can provide a coverage of the oligonucleotide set for a plurality of nucleic acid sequences, can analyze specificity of the oligonucleotide set, and can modify the sequences of the oligonucleotides included in the oligonucleotide set for the improvement in specificity. According to the present invention, the specificity analysis results can be compared between an oligonucleotide set of an existing product and an oligonucleotide set of a new product, and the specificity change of the oligonucleotide set can be easily monitored.
Disclosed is a sampling kit for determining respiratory infection, the kit including: a first vessel containing a washing-out solution; and a second vessel containing a transport medium containing a deactivating agent, so that sampling corresponding to a pre-analytical stage in a protocol for determination of the presence or absence of respiratory infection pathogens can be attained easily, safely, and stably, and self-sampling of a respiratory infection pathogen can be attained.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
A61B 10/00 - Other methods or instruments for diagnosis, e.g. for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
13.
METHOD FOR MANAGING RESPIRATORY INFECTIONS THROUGH MOBILE TERMINAL MANAGEMENT SYSTEM INCLUDING CENTRAL MANAGEMENT SERVER, SERVER, AND COMPUTER READABLE STORAGE MEDIUM
The present invention relates to a method for managing respiratory infections, a server, and a computer readable storage medium which conducts a respiratory infection test to a client who wants to the respiratory infection tests, receives the client's location information, and provides respiratory infection management information generated using the test result of the respiratory infection and client's location information for other clients so that it may minimize the spread of infection and efficiently quarantine against the respiratory infections while maintaining social and economic activities of the public.
G16H 10/65 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records stored on portable record carriers, e.g. on smartcards, RFID tags or CD
An embodiment provides an optical signal detection device including a sample holder configured to accommodate a plurality of samples, a light source module including a plurality of light source units dedicated to each sample area to irradiate light to a plurality of sample areas on the sample holder, a filter module including a plurality of movable moving units for filtering light from the light source unit and a detection module configured to detect emission light of the sample.
The present invention relates to technologies for preparing an optimal combination of oligonucleotide sets used to simultaneously detect a plurality of target nucleic acid molecules. Unlike a conventional method of checking whether a dimer is formed in all candidate combinations of oligonucleotide sets, the present invention is capable of providing a combination of oligonucleotide sets used to detect a plurality of target nucleic acid molecules with speed and accuracy, by replacing only an oligonucleotide set with dimer formation in a first reference combination of oligonucleotide sets to provide, as a new reference combination, a combination with a reduction in dimer formation compared with the first reference combination, and replacing only an oligonucleotide set with dimer formation in the new reference combination to provide a combination with all dimers removed.
The disclosure relates to a method for detecting a target nucleic acid in a sample using a nucleic acid reaction detection device comprising a plurality of reaction areas independent from each other. According to the disclosure, the method may simultaneously perform two or more nucleic acid reactions which are carried out with different protocols and independently measure emitted optical signals, thereby detecting the target nucleic acid.
Embodiments of the disclosure relate to a device and method for detecting light in a plurality of independent reaction regions. According to the disclosure, a light detection device includes a plurality of thermally independent reaction regions, a movable optical module for irradiating the reaction regions with lights of a plurality of wavelengths, detectors for detecting lights emitted from the reaction regions, and a controller controlling the reaction regions, the optical module, and the detectors.
The present invention relates to the detection of a target nucleic acid sequence by a PTOCE (PTO Cleavage and Extension) assay. The present invention detects a target nucleic acid sequence in which the PTO (Probing and Tagging Oligonucleotide) hybridized with the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized with the CTO (Capturing and Templating Oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex. The extended duplex provides signals (generation, increase, extinguishment or decrease of signals) from labels indicating the presence of the extended duplex and has adjustable Tm value, which are well adoptable for detection of the presence of the target nucleic acid sequence.
Provided a thermal cycler. In a case in which plurality of heat sinks participate in thermal control of a plurality of thermally independent sample holders for reliable nucleic acid reactions of the plurality of sample holder, a barrier is present between the adjacent heat sinks.
The disclosure relates to a computer-implemented method, system, and storage medium for collaborative development of a reagent for detection of the target nucleic acid. According to the disclosure, the method, system, and storage medium automatically match the developer and technology provider suitable for the characteristics of collaborative development in response to a request for collaborative development, thereby increasing the efficiency of collaborative development.
The present invention relates to technologies for determining a designable region of oligonucleotides in a plurality of target nucleic acid sequences having sequence similarity. In determining a conservative region in a plurality of nucleic acid sequences, unlike the conventional methods which is an empirical and manually selected methods, the present invention provides a more logical and efficient method by adopting a strategy of generating oligonucleotide sticks having sequence information about non-conservative positions within a predetermined allowable number or a minimum number of sequence patterns within a predetermined allowable number of sequence patterns while having a predetermined length or more from the alignment results of a plurality of target nucleic acid sequences and has excellent speed and accuracy.
The present disclosure relates to a light module comprising a plurality of light sources emitting light to excite samples; a light source wheel accommodating the plurality of light sources; a plurality of filters filtering light emitted by the light sources; a filter wheel accommodating the plurality of filters; and a motor rotating the light source wheel and the filter wheel.
The present invention relates to a method for determining the presence or absence of M. tuberculosis, M. bovis, and M. bovis BCG in a sample comprising a nucleic acid molecule. A method according to the present invention can detect the individual presence of M. tuberculosis, M. bovis, and M. bovis BCG, and the co-presence of two thereof.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
24.
METHOD FOR DETECTING A TARGET ANALYTE IN A SAMPLE USING AN S-SHAPED FUNCTION FOR A SLOPE DATA SET
The present invention relates to the detection of a target analyte in a sample using an S-shaped function for a slope data set. The method of the present invention comprises obtaining a first data set representing a growth curve by an amplification reaction for the target analyte; wherein said first data set includes a plurality of data points, each having a cycle number and a signal value at the cycle number; calculating a slope value at each cycle number for the first data set to obtain a second data set; wherein said second data set includes a plurality of data points, each having a cycle number and a slope value at the cycle number; calculating an S-shaped function that approximates a selected portion of the second data set; and using the S-shaped function to detect the target analyte in a sample.
The present invention provides a method for providing a target nucleic acid sequence data set of a target nucleic acid molecule. The larger number of synonyms are retrieved from reliable sources than that of synonyms extracted by user experience. Thus, the target nucleic acid sequence data group of the target nucleic acid molecule retrieved based on the larger number of synonyms may cover various variant sequences of the target nucleic acid molecule.
The present invention relates to a method for analyzing a sample. In particular, the present invention relates to a method for analyzing a sample and a method for correcting a raw data set of an amplification reaction. The present invention for analyzing a sample prevents from determining cycles based on false signals usually observed in a multitude of reactions and processes, thereby much more accurately obtaining information for analyzing a sample.
G16B 40/00 - ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
27.
Method and device for controlling detection-composition preparation instrument
Provided is a method and device for controlling a preparation instrument that prepares a detection composition used for detecting a target nucleic acid molecule in a specimen using an integrated instruction file.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G05B 19/4155 - Numerical control (NC), i.e. automatically operating machines, in particular machine tools, e.g. in a manufacturing environment, so as to execute positioning, movement or co-ordinated operations by means of programme data in numerical form characterised by programme execution, i.e. part programme or machine function execution, e.g. selection of a programme
The present invention relates to differentiating signals of interest for target nucleic acid sequences. The present invention permits to obtain an individual signal value (i.e., variable) contained in a total signal detected at detection temperatures by using mathematical equations. The present invention based on equation-solving approach enables to obtain the individual signal value in a systematical manner, thereby providing analysis results in much more accurate and convenient manner.
G16B 40/00 - ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
29.
Analytical signal for determination of the presence of a target nucleic acid sequence
The present invention relates to a method for providing an analytical signal for determination of the presence of a target nucleic acid sequence in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention allows detection of a target nucleic acid sequence in a more accurate, effective and reproducible manner, by removing or adjusting a signal region that may affect the detection of a target nucleic acid sequence.
The present invention relates to a method for detecting a nucleic acid of a gut microorganism in a sample using a nucleic acid of a bacterium as an internal control nucleic acid selected from a normal gut flora, and to a composition for nucleic acid amplification used in the method. The internal control according to the present invention is present in the sample from the beginning, and thus there is no inconvenience of separately adding an internal control after the sample collection process, and may be used as an internal control for the sample collection process, an internal control for the nucleic acid extraction process, and an internal control for the nucleic acid amplification process. In addition, the presence or absence of the nucleic acid of the gut microorganism in the sample may be detected with a high accuracy through the minimization of false negative and false-positive determinations by using the nucleic acid of the bacterium as the internal control selected from the normal gut flora.
The present invention relates to the evaluation of the performance of a component using a pair of dimer-forming primers. The method using the pair of dimer-forming primers according to the present invention can be used not only for evaluating the performance of components including a nucleic acid polymerase but also as an internal control in the detection of a target nucleic acid sequence.
The present invention relates to a method for predicting the melting temperature (Tm) of an oligonucleotide, in particular a primer or probe, in a PCR or hybridization assay. The method of present invention can accurately predict the Tm of an oligonucleotide in various reaction environments using the equations for Tm calculation, the equation including parameter values optimized for the reaction environment in which the oligonucleotide is to be used.
G16B 40/00 - ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
33.
Method And Apparatus For Detecting A Plurality Of Target Nucleic Acid Sequences In Sample
The method of the present invention enables efficient detection of a plurality of target nucleic acid sequences in one detection channel, by obtaining a data set of cycle/signal-change value.
The present invention relates to a method and device for determining the presence or absence of a target analyte in a sample. The present invention may analyze the target analyte without false results, especially false positive results by using a fitting accuracy of a nonlinear function to a data set as a direct indicator for target analyte analysis.
The present invention relates to the detection of a target nucleic acid sequence by a PTOCE-E (PTO Cleavage and Extension-dependent Extension) assay. The PTOCE-E assay of the present invention can reduce the non-target signal and increase the target signal as compared with the conventional PTOCE and PCE-SH methods, thereby enabling more accurate detection of the target nucleic acid sequence.
An amplification data display method according to the present invention comprises the steps of: displaying a plate display area and an integrated data display area; displaying, the plate display area, plates where nucleic acid amplification reactions have been performed; and displaying amplification data in the integrated data display area, wherein: two or more plates are displayed in the plate display area; each of the plates includes a plurality of reaction wells; the amplification data are generated from the reaction wells by the nucleic acid amplification reactions; and amplification data for a plurality of reaction wells selected from the reaction wells of two or more of the displayed plates are displayed in the integrated data display area.
The present invention relates to novel modules for transferring magnetic beads, an automated system comprising the same and a method for extracting nucleic acids using the same. The specifically designed magnet module and cover module of the present invention can be employed in the automated liquid handling apparatus by means of pre-existing moving modules (e.g., pipettor module) of the apparatus. The present invention enables a bead transfer-type method for extracting nucleic acids to be performed in an automated manner on the automated liquid handling apparatus. The present invention provides advantages of higher level of automation, more reduced cost and no need for another separate liquid handling apparatus compared to the conventional bead transfer-type method usually performed in the small apparatus designed to be used only for this bead transfer-type method. Also, the present method has the merits of more shortened reaction time compared to the conventional liquid transfer-type method.
The present invention relates to optimization logic for preparing an optimal introduction of degenerate bases and/or universal bases into an oligonucleotide used to detect a plurality of target nucleic acid sequences, in a completely different approach from conventional methods, i.e., empirical and manual methods. In addition, the optimization logic of the present invention may be used in (i) the preparation of an oligonucleotide into which a limited number of degenerate bases and/or universal bases are introduced for detecting a plurality of target nucleic acid sequences with a maximum target coverage, and (ii) the determination of a probing region in a plurality of target nucleic acid sequences.
C12Q 1/683 - Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
40.
Methods for preparing optimal combination of oligonucleotides
The present invention relates to optimization logic for preparing an optimal combination of oligonucleotides hybridized with a plurality of target nucleic acid sequences, in a completely different approach from conventional methods, i.e., empirical and manual methods. In addition, the optimization logic of the present invention may be used to (i) preparing an oligonucleotide combination used to detect a plurality of target nucleic acid sequences with a target coverage of interest, (ii) selecting target nucleic acid sequences to be detected by a multiplex target detection with a highest target coverage by using a limited number of oligonucleotides, and (iii) determining a conserved region in a plurality of target nucleic acid sequences.
The present invention relates to an apparatus for performing a nucleic acid amplification reaction and a fluorescence detection device for reaction analysis. The nucleic acid amplification apparatus of the present invention uses a plurality of blocks having different reaction temperatures by independent temperature control and the movement between the blocks is performed along sliding recesses formed in the blocks, enabling to greatly shorten the total amplification time (TAT). In the fluorescence detection device of the present invention, the positions of the light source and the photodetector are very unique for the reaction vessel in which an excitation light is provided and an emission light is generated.
The present invention relates to a method for amplifying at least three target nucleic acid molecules with reduced primer dimer formation in a multiplex amplification reaction. The method of present invention can inhibit primer dimer formation and hence generation of nonspecific amplification products in an effective manner in a multiplex amplification reaction for at least three target nucleic acid molecules.
The present invention relates to technologies for preparing a tagging oligonucleotide. By analyzing exquisitely a non-complementarity level of a first tagging part, the first aspect of the present invention permits to more efficiently and easily select a suitable tagging sequence among a multitude of sequences generated theoretically. In addition, according to the second aspect of the present invention, when a nucleotide sequence for a tagging portion is first selected, one or more regions in a target nucleic acid sequence having a non-complementarity level to the nucleotide sequence for the tagging portion are found, and then a nucleotide sequence for a targeting portion is determined, tagging oligonucleotides for detecting various target nucleic acid sequences can prepared by using the fewest number of nucleotide sequences for the tagging portion and a third template.
The present invention relates to technology for preparing oligonucleotides for detecting a target nucleic acid molecule in a sample. Unlike the conventional methods, the present invention provides a first oligonucleotide candidate group designed appropriately for the first selected nucleotide sequence of the target nucleic acid molecule as a standard instead of simultaneously referring to all of the sequences exhibiting the genetic diversity. Then, an optimal oligonucleotide capable of accurately detecting a target nucleic acid molecule exhibiting genetic diversity in a sample is provided by using the first oligonucleotide candidate group.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
G16B 25/00 - ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
G16B 25/20 - Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
45.
Detection of abnormal signal using two or more datasets
The present invention relates to a method for de-tecting an abnormal signal using two or more datasets. The present invention makes it possible to detect abnormal signals based on characteristics of abnormal signals commonly occurring in two or more datasets, which is effectively applied to datasets obtained by a multiplex PCR method.
The present invention relates to a method for detecting a target analyte in a sample using a signal change-amount data set and its reconstructed data set. According to the present invention, a data set amendment for target analyte detection such as baselining and smoothing of a data set can be easily achieved without complicated steps such as setting a baseline region.
The present invention relates to a method for reducing a noise level of a data set for a target analyte in a sample. The present invention can reduce a noise level of a data set to a proper level conveniently by applying a noise-reduction ratio to the data set thereby the possibility of false positive may be reduced effectively. According to the present invention, the calibrated data set is obtained by using the noise-reduction ratio, so that the noise level of a data set is reduced without change of signal ratio between the data point in the data set.
The present invention relates to extraction of a signal for a target nucleic acid sequence from signals for two target nucleic acid sequences in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention using an amended reference value as well as an initial reference value can lead to increasing the detection accuracy in methods for detecting target nucleic acid sequences using different detection temperatures and reference values.
The present invention relates to a method for calibrating a data set of a target analyte in a sample, wherein a normalization coefficient for calibrating the data set is provided by using a reference value, a reference cycle and the data set, and the calibrated data set is obtained by applying the normalization coefficient to the signal values of the data set. The present method is very effective in removing the inter- and intra-instrument signal variations of data sets. Furthermore, since the present method can be configured in software, the instant method is capable of being applied universally to various analytical instruments (e.g., a real-time PCR instrument) regardless of manufacturer. Accordingly, the method by the present invention would be very useful in diagnostic data analysis.
G16B 40/00 - ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
50.
Differentiation of signals for target nucleic acid sequences
The present invention relates to differentiating signals of interest for target nucleic acid sequences. The present invention permits to obtain an individual signal value (i.e., variable) contained in a total signal detected at detection temperatures by using mathematical equations. The present invention based on equation-solving approach enables to obtain the individual signal value in a systematical manner, thereby providing analysis results in much more accurate and convenient manner.
G16B 40/00 - ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
51.
MULTIPLE DATASET ANALYSIS FOR DETERMINING THE PRESENCE OR ABSENCE OF TARGET ANALYTE
The present invention relates to the determination of the presence or absence of a target analyte by a Multiple Dataset Analysis (MDA). The present invention can dramatically reduce errors (particularly, false positive errors) in determination of the presence or absence of a target analyte, by using two or more different types of datasets from an amplification reaction.
G06F 19/22 - for sequence comparison involving nucleotides or amino acids, e.g. homology search, motif or Single-Nucleotide Polymorphism [SNP] discovery or sequence alignment
The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
The present invention relates to the detection of a target nucleic acid sequence by a PCE-SH (PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Hybridization) assay. The present invention does not use probes to be hybridized with target nucleic acid sequences for providing target signals. Interestingly, the present invention uses probes (signaling oligonucleotides) to be hybridized with the extended strand formed in a target-dependent manner in which the extended strand is synthesized using the CTO artificially selected as templates.
The present invention relates to a method for lyophilizing a composition for multiple target nucleic acid sequence amplification reaction and a lyophilizate prepared by the method. The present method is very effective in lyophilizing a composition containing a high concentration of oligonucleotides. The lyophilizates prepared by the present invention exhibits excellent properties in terms of both sensitivity and specificity, equivalent performance capacity to conventional liquid formulation and furthermore remarkable storage stability. Accordingly, the lyophilizates prepared by the present invention would be very useful in diagnosis.
The present invention relates to detection of target nucleic acid sequences using different detection temperatures and reference values. The present invention employing different detection temperatures and reference values enables to detect a plurality of target nucleic acid sequences in conventional real-time manners even with a single type of label in a single reaction vessel.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
G06F 19/22 - for sequence comparison involving nucleotides or amino acids, e.g. homology search, motif or Single-Nucleotide Polymorphism [SNP] discovery or sequence alignment
The present invention relates to a target discriminative probe (TD probe) and its uses or applications. The TD probe is hybridized with a target nucleic acid sequence through both of the 5′-second hybridization portion and the 3′-first hybridization portion. When the TD probe is hybridized with a non-target nucleic acid sequence, both the 5′-second hybridization portion and the separation portion are not hybridized with the non-target nucleic acid sequence such that both portions form a single strand due to its low Tm value. As such, the TD probe exhibits distinctly different hybridization patterns for each of the target and the non-target nucleic acid sequence, discriminating the target nucleic acid sequence from the non-target nucleic acid sequence with much higher specificity.
The present invention relates to detection of target nucleic acid sequences using different detection temperatures. The present invention employing different detection temperatures enables to detect a plurality of target nucleic acid sequences in conventional real-time manners even with a single type of label in a single reaction vessel. The conventional technologies detect a plurality of target nucleic acid sequences by a melting analysis after target amplification. Unlikely, the present invention does not require a melting analysis after target amplification, such that the time for analysis is greatly reduced.
C12Q 1/683 - Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
59.
Detection of a target nucleic acid sequence using two different detection temperatures
The present invention relates to detection of a target nucleic acid sequence in a sample using two different detection temperatures. The present invention using difference between signals detected at two detection temperatures enables to decrease well-to-well variation or sample-to-sample variation generated in real-time PCR processes in more convenient and effective manner.
The present invention relates to a method for quantifying a target nucleic acid sequence by use of a common internal control. The present invention allows to determine an absolute initial amount of a target nucleic acid sequence with no use of a standard curve. The present invention amplifies not only a control reaction mixture comprising a known-amount standard and an internal control but also a sample reaction mixture comprising an internal control identical to the internal control in the control reaction mixture and the target nucleic acid sequence to be quantified. In the present invention, a relative ratio of the amount of the standard and the amount of the target nucleic acid sequence is calculated by using the internal control and then an initial amount of the target nucleic acid sequence is determined by using a known amount of the standard.
C12Q 1/683 - Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
The present invention is generally drawn to a novel method for determining a SNP (single nucleotide polymorphism) genotype using a PTO-SNV (Probing and Tagging Oligonucleotide for Single Nucleotide Variation). The present invention provides novel protocols for SNP genotyping in which only one allele-specific oligonucleotide permits in a SNP genotyping reaction to determine whether a target nucleic acid sequence to be analyzed is homozygous or heterozygous for the SNP allele of interest or has no SNP allele of interest.
The present invention relates to the detection of a target nucleic acid sequence from a DNA or a mixture of nucleic acids by a PCE-hCTO (PTO Cleavage and Extension using hCTO) assay on a solid phase. According to the present invention, the extended duplex is formed in a liquid phase in a target-dependent manner and then its presence is detected on a solid phase. Since hCTO is not immobilized onto a solid phase, the extended duplex is more effectively formed in a liquid phase.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
C12N 9/00 - Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12Q 1/6832 - Enhancement of hybridisation reaction
The present invention relates to the detection of a target nucleic acid sequence by a PCE-IH (PTO Cleavage and Extension-Dependent Immobilized Oligonucleotide Hybridization) assay on a solid phase. The present invention firstly hybridizes the PTO with a target nucleic acid sequence, forms the extended strand in a target-dependent manner by using the CTO having artificially selected sequence as templates and finally hybridizes the extended strand with the IO immobilized on a solid phase. In other words, the present invention employs a series of reactions including PTO hybridization and cleavage, CTO hybridization and extension and IO hybridization, which is responsible for the highly enhanced specificity of the present invention.
The present invention relates to the detection of a nucleotide variation on a target nucleic acid sequence using an amplification blocker and a VD-PTOCE (Variation Detection by PTO Cleavage and Extension) assay. The present invention is significantly effective in the detection of a minority mutation in an excess of wild-type DNA.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
The present invention relates to a method for quantifying a target nucleic acid sequence performed in such a manner that at least two cycles in the nucleic acid amplification subject to melting peak analysis are predetermined before the nucleic acid amplification and melting peak analyses are performed for the at least two predetermined cycles, followed by quantifying the target nucleic acid sequence using data values from the melting peak curve (e.g., the presence or absence, height and area).
The present invention relates to the detection of a target nucleic acid sequence by a PCE-NH (PTO Cleavage and Extension-Dependent Non-Hybridization) assay. The present invention adopts the occurrence of the inhibition of the hybridization between the HO with the CTO by the formation of the target-dependent extended duplex. Therefore, the present invention may detect target sequences even when the HO is not cleaved. In this regard, the design of the 5′-tagging portion of PTO, CTO and HO sequences may be readily performed and the conditions for reactions may be also easily established. In addition, the detection of the hybrid between the CTO and the HO may be performed in a different vessel from that for the extension of the CTO.
The present invention is generally drawn to a novel method and a kit for detecting nucleotide variations by a PTOCE (PTO Cleavage and Extension) assay with PTO-NV. Furthermore, the present invention is directed to a novel method and a kit for detecting a nucleotide variation on a target nucleic acid sequence by a PTOCE assay with PTO-NV having a non-base paring moiety.
The present invention relates to the detection of a target nucleic acid sequence by a PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Cleavage assay (PCE-SC assay). The present invention is carried out in such a manner that the extended strand is produced on the CTO having arbitrary sequences as templates depending on the presence of target nucleic acid sequences and in turn the SO as probes is hybridized with the extended strand to give signal. The present invention employs a series of reactions including PTO hybridization and cleavage, CTO hybridization and extension, and SO hybridization and cleavage, which is responsible for the highly enhanced specificity of the present invention.
The present invention relates to the detection of a target nucleic acid sequence by a PCE-SH (PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Hybridization) assay. The present invention does not use probes to be hybridized with target nucleic acid sequences for providing target signals. Interestingly, the present invention uses probes (signaling oligonucleotides) to be hybridized with the extended strand formed in a target-dependent manner in which the extended strand is synthesized using the CTO artificially selected as templates.
The present invention relates to the detection of a target nucleic acid sequence by a POCH (PO Cleavage and Hybridization) assay on a solid substrate. The present invention detects the target nucleic acid sequence by use of in which the PO (Probing Oligonucleotide) hybridized with the target nucleic acid sequence is cleaved and the cleavage of the PO is detected by hybridization with the CO (Capturing Oligonucleotide). In the present invention, an uncleaved PO is hybridized with the CO immobilized onto the solid substrate. The designs of the PO and the CO are convenient and the optimization of reaction conditions is routinely easy in the present invention. Where the detection of signal on the solid substrate is continuously performed along with repetition of cleavage of the POs in the present invention, the number of the POs cleaved is increased upon the repetition number of the cleavage reaction and the signal is changed in parallel with the number of the POs cleaved. Then, the target nucleic acid sequence can be detected in a real-time manner. In contrast, the change of the signal is not observed in the absence of the target nucleic acid sequence.
The present invention relates to the detection of a target nucleic acid sequence by a PCEC (PTO Cleavage and Extension-Dependent Cleavage) assay. The present invention is characterized by generating a cleavage site for a nucleolytic enzyme on the extended duplex of which the formation is dependent on the presence of a target nucleic acid sequence. The present invention detects the occurrence of the cleavage of the extended duplex, thereby determining the presence of the target nucleic acid sequence.
The present invention relates to a novel method for detection of target nucleic acid sequences on a solid phase using dual-labeled immobilized probes and its resistance to a 5′ to 3′ exonuclease activity of a DNA polymerase. Because the label is remained on the solid substrate by resistance to nucleases due to labeling of a base component the internal nucleotide, the present invention requires no consideration of a suitability of position of the label for remaining on the solid substrate. The present invention ensures to minimize background signal by positioning labels at a site on probes suitable to maximize quenching efficiency of the dual label system, since it permits to freely determine the position of the internal label on probes.
The present invention relates to a novel method for detection of target nucleic acid sequences by cyclic exonucleolytic reactions (CER) or exonucleolytic reactions (ER) using single-labeled immobilized probes on a solid phase. The present invention enables to detect target nucleic acid sequences on a solid phase using single-labeled systems. Comparing with multiple-labeled systems such as dual labeling, the present invention using single-labeled probes has excellent advantages in light of convenience and cost effectiveness in probe design and preparation. Furthermore, the measurement of changes of the signal decrease during reactions is responsible for more accurate qualitative and quantitative analysis of target nucleic acid sequences.
The present invention relates to the detection of a target nucleic acid sequence by a PTOCE (PTO Cleavage and Extension) assay. The present invention detects a target nucleic acid sequence in which the PTO (Probing and Tagging Oligonucleotide) hybridized with the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized with the CTO (Capturing and Templating Oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex. The extended duplex provides signals (generation, increase, extinguishment or decrease of signals) from labels indicating the presence of the extended duplex and has adjustable Tm value, which are well adoptable for detection of the presence of the target nucleic acid sequence.
The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
The present invention relates to the detection of a target nucleic acid sequence in a real-time manner using a target signal generating primer (TSG primer) having dual interactive labels. The present invention allows for both target amplification and signal amplification by introducing dual interactive labels into a primer used in PCR reactions, ensuring real-time target detection by PCR reactions without the use of complicated oligonucleotides. The present invention could be free from the troublesome matters and shortcomings associated with conventional real-time PCR methods. The present invention allows for successful real-time target detection by using only a labeled primer. Also, the present invention can obtain strong signals indicative of the presence of target nucleic acid sequences in both a liquid phase and solid phase.
The present invention relates to the detection of a target nucleic acid sequence using a target hybridization and detection primer (THD primer). The present invention allows for both a target amplification and a signal amplification by introducing a label into a primer used in PCR reactions, ensuring a real-time target detection by PCR reaction by no use of complicated oligonucleotides. The present invention could completely be free from the troublesome matters and shortcomings associated with conventional real-time PCR methods. The present invention allows for successful real-time target detection by using only a labeled primer. This feature makes it possible that the present invention exhibits excellent real-time target detection in multiplex manner.
The present invention relates to the detection of a target nucleic acid sequence by a cyclic exonucleolytic reaction. The present method enabling to generate signals by probe digestion with no help of primers and to amplify signals with no help of simultaneous target amplification reactions may enable to detect multiple target sequences without any problems accounted in the conventional real-time PCR methods such as false positive signals and difficulties in oligonucleotides (primer and probe) selection and reaction condition optimization.
The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance.
The present invention relates to a method for amplifying an unknown nucleotide sequence adjacent to a known nucleotide sequence, which comprises the step of performing an amplification of the unknown nucleotide sequence using a DNA walking annealing control primer (DW-ACP) and a target specific primer (TSP) hybridizable with a site on the known nucleotide sequence.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
83.
Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance.
The present invention relates to a method for amplifying an unknown nucleotide sequence adjacent to a known nucleotide sequence, which comprises the step of (a) performing a primary amplification of said unknown nucleotide sequence using a DNA walking annealing control primer (DW-ACP) and a first target-specific primer; in which said step (a) comprises: (a-1) performing a first-stage amplification of said unknown nucleotide sequence at a first annealing temperature, comprising at least one cycle of primer annealing, primer extending and denaturing using a first degenerate DW-ACP containing a degenerate random nucleotide sequence to hybridize with said unknown nucleotide sequence and a hybridizing nucleotide sequence substantially complementary to a site on said unknown nucleotide sequence; and (a-2) performing a second-stage amplification at a second annealing temperature to render said first degenerate DW-ACP not to function as a primer.
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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