Abstract

Provided herein is a method of chemically modifying a polypeptide or polynucleotide monomer. The monomer may be a monomer of a protein pore such as a monomeric or oligomeric protein pore. Also disclosed are related monomers and oligomers and methods of using the disclosed pores in characterising analytes.

IPC Classes  ?

• C07K 1/107 - General processes for the preparation of peptides by chemical modification of precursor peptides
• C07K 1/13 - Labelling of peptides
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• B82Y 40/00 - Manufacture or treatment of nanostructures
• G01N 15/00 - Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research. Reagents for use in automated test systems; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants; reagents for medical and veterinary diagnosis and medical and veterinary research; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary, medical, medical research or forensic use; reagents for immunoassay analysers; reagents for testing aqueous solutions; reagents for testing for susceptibility to allergies; reagents for use in biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; non-aqueous media; surfactants; silicone oils; enzymes; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes. Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; laboratory and data processing apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, medical diagnosis, measurement of ions; portable laboratory and data processing apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, medical diagnosis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents for scientific and scientific research use; reaction units containing chemical or biological reagents, being laboratory equipment other than for medical use; nanopore sensing devices; parts and fittings for all the aforesaid goods. Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; medical and veterinary apparatus using electrowetting technology; apparatus and instruments for the preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, for medical purposes; nanopore sensing devices being medical apparatus and instruments; parts and fittings for all the aforesaid goods. Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business services; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services; retail services connected with the sale of data analysis products and services and data streaming products and services; retail services connected with the sale of electronic sensing and measuring equipment, apparatus and instruments; retail services connected with the sale of sequencing equipment, apparatus and instruments; retail services connected with the sale of data processing equipment, apparatus and instruments; retail services connected with the sale of scientific equipment, apparatus and instruments; retail services connected with the sale of surgical, medical and veterinary equipment, apparatus and instruments; retail services connected with the sale of reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail services connected with the sale of chemicals and enzymes; retail services connected with the sale of chemicals and enzymes for scientific, medical or veterinary purposes; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail services connected with the sale of goods for use in a laboratory; information, advisory and consultancy services relating to the aforesaid. Education services; training services; training courses, workshops classes, symposiums and seminars; training courses, workshops, classes, symposiums and seminars regarding DNA/RNA sequencing, data analysis and conducting scientific tests and experiments; training courses, workshops, classes, symposiums and seminars regarding the use of scientific and medical apparatus, instruments and equipment; arranging and conducting conferences; information, advisory and consultancy services relating to the aforesaid. Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; research and development of new products for others in the nature of the synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of technical data, including scientific and medical data; computerised analysis of data (terms considered too vague by the international bureau - rule 13 (2) (b) of the common regulations); overseeing, monitoring and managing scientific and medical research experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical research data; design of scientific experiments; analysis of technical data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services for scientific research purposes; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services for scientific research purposes; genome sequencing services and the analysis and evaluation of data resulting therefrom; preparation of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid. Medical and veterinary diagnostic services; medical and veterinary testing services; genetic testing for medical and veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular testing and analysis services and DNA/RNA sequencing services for medical, veterinary, agricultural and aquacultural purposes; information, advisory and consultancy services relating to the aforesaid.

Abstract

A method is provided comprising, for each respective pair of one or more target nucleic acids and one or more reference nucleic acids, generating an alignment between a segment of the respective target nucleic acid a segment of the respective reference nucleic acid, wherein the respective reference nucleic acid comprises a respective barcode sequence and a respective first context sequence. For each pair, a sequence similarity is determined between a scoring region of the reference nucleic acid and a corresponding segment of the target nucleic acid. For each pair, it is determine whether the target nucleic acid comprises the barcode sequence of the reference nucleic acid based on the sequence similarity between the target nucleic acid and the scoring region of the reference nucleic acid.

IPC Classes  ?

• G16B 30/10 - Sequence alignment; Homology search
• C12Q 1/6809 - Methods for determination or identification of nucleic acids involving differential detection

Abstract

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sense a fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• G01N 15/12 - Coulter-counters

Abstract

There is disclosed a nanopore support structure comprising a wall layer comprising walls defining a plurality of wells, and overhangs extending from the walls across each of the wells, the overhang defining an aperture configured to support a membrane suitable for insertion of a nanopore. There is further disclosed a nanopore sensing device comprising a nanopore support structure, and methods of manufacturing the nanopore support structure and the nanopore sensing device.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

The invention provides a method of selectively characterising polynucleotides of a desired property, such as length, using a nanopore, based on the translocation of the polynucleotide through or across the nanopore. Kits and systems for use in such methods are also provided. The methods of the invention are particularly suitable for sequencing polynucleotides such as DNA.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing

Abstract

Provided herein is a method of moving a double-stranded polynucleotide with respect to a nanopore using a motor protein. The method allows a portion of the polynucleotide to be interrogated by the pore multiple times. Also provided are polynucleotide adapters and kits comprising such adapters. The methods find use in characterising polynucleotides, for example in sequencing.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing

Abstract

Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non­-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research; reagents for medical and veterinary research; nucleic acid amplification reagents for medical research use. Reagents for use in automated test systems for medical or veterinary use; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants for medical or veterinary use; reagents for medical and veterinary diagnosis; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary, medical or forensic use; reagents for immunoassay analysers for medical or veterinary use; reagents for testing aqueous solutions for medical or veterinary use; reagents for testing for susceptibility to allergies; reagents for use in biological processing for medical or veterinary use; reagents for use in nucleic acid characterisation and nucleic acid sequencing for medical or veterinary use; reagents for protein identification and analysis for medical or veterinary use; reagents for formation of amphipathic membranes for medical or veterinary use; buffer solutions for medical or veterinary use; aqueous solvents for medical or veterinary use; non-aqueous solvents for medical or veterinary use; non-aqueous media for medical or veterinary use; surfactants for medical or veterinary use; silicone oils for medical or veterinary use; enzymes for medical or veterinary use; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes. Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; portable laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; cartridges being laboratory and scientific apparatus for holding biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents being laboratory equipment other than for medical use; reaction units containing chemical or biological reagents, being laboratory equipment, other than for medical use; Nanopore sensing devices; parts and fittings for all the aforesaid goods. Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; medical and veterinary apparatus using electrowetting technology; automated apparatus and instruments for the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, for medical and veterinary purposes; Nanopore sensing devices; apparatus and instruments for medical diagnosis; portable apparatus and instruments for medical diagnosis; parts and fittings for all the aforesaid goods. Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business services; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services connected with the sale of data analysis products and services and data streaming products and services; retail services connected with the sale of electronic sensing and measuring equipment, apparatus and instruments; retail services connected with the sale of sequencing equipment, apparatus and instruments; retail services connected with the sale of data processing equipment, apparatus and instruments; retail services connected with the sale of scientific equipment, apparatus and instruments; retail services connected with the sale of surgical, medical and veterinary equipment, apparatus and instruments; retail services connected with the sale of reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail services connected with the sale of chemicals and enzymes; retail services connected with the sale of chemicals and enzymes for scientific, medical or veterinary purposes; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; information, advisory and consultancy services relating to the aforesaid. Training courses, workshops, classes, symposiums and seminars regarding the use of scientific and medical apparatus, instruments and equipment in the field of DNA/RNA related diagnostics, DNA/RNA sequencing, medical testing and related diagnostics; arranging and conducting conferences in the field of DNA/RNA related diagnostics, DNA/RNA sequencing, medical testing and related diagnostics; information, advisory and consultancy services relating to the aforesaid. Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; synthesising services of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific testing purposes; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of data, including scientific and medical research data; computerised analysis of data; overseeing, monitoring and managing scientific and medical research experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical research data; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid. Medical and veterinary diagnostic services; medical and veterinary testing services; genetic testing for medical and veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynuclear acid and molecular testing and analysis services and DNA/RNA sequencing services for medical, veterinary, agricultural and aquacultural purposes; information, advisory and consultancy services relating to the aforesaid.

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research. Reagents for use in automated test systems for medical or veterinary purposes; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants; reagents for medical and veterinary diagnosis and medical and veterinary research; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary, medical, medical research or forensic use; reagents for immunoassay analysers; reagents for testing aqueous solutions for medical or veterinary purposes; reagents for testing for susceptibility to allergies; reagents for use in biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing for medical or veterinary purposes; reagents for protein identification and analysis for medical or veterinary purposes; reagents for formation of amphipathic membranes for medical or veterinary purposes; buffer solutions for medical or veterinary purposes; aqueous solvents for medical or veterinary purposes; non-aqueous solvents for medical or veterinary purposes; non-aqueous media for medical or veterinary purposes; surfactants for medical or veterinary purposes; silicone oils for medical or veterinary purposes; enzymes for medical or veterinary purposes; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes. Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; portable laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents; reaction units containing chemical or biological reagents; Nanopore sensing devices; parts and fittings for all the aforesaid goods. Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; electrowetting devices for medical and veterinary purposes; apparatus and instruments for automating the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; reaction units containing chemical reagents, for medical purposes; parts and fittings for all the aforesaid goods. Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business services; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services connected with the sale of data analysis products and services and data streaming products and services; retail services connected with the sale of electronic sensing and measuring equipment, apparatus and instruments; retail services connected with the sale of sequencing equipment, apparatus and instruments; retail services connected with the sale of data processing equipment, apparatus and instruments; retail services connected with the sale of scientific equipment, apparatus and instruments; retail services connected with the sale of surgical, medical and veterinary equipment, apparatus and instruments; retail services connected with the sale of reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail services connected with the sale of chemicals and enzymes; retail services connected with the sale of chemicals and enzymes for scientific, medical or veterinary purposes; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail services connected with the sale of goods for use in a laboratory; information, advisory and consultancy services relating to the aforesaid. Education services; training services; training courses, workshops, classes, symposiums and seminars; training courses, workshops, classes, symposiums and seminars regarding DNA/RNA sequencing, data analysis and conducting scientific tests and experiments; training courses, workshops, classes, symposiums and seminars regarding the use of scientific and medical apparatus, instruments and equipment; arranging and conducting conferences; information, advisory and consultancy services relating to the aforesaid. Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; services relating to the synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of technical data, including scientific and medical data; computerised analysis of data; overseeing, monitoring and managing scientific and medical experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical data; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid. Medical and veterinary diagnostic services; medical and veterinary testing services; genetic testing for medical and veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular testing and analysis services and DNA/RNA sequencing services for medical, veterinary, agricultural and aquacultural purposes; information, advisory and consultancy services relating to the aforesaid.

Goods & Services

Scientific and technological services and research and design relating thereto, namely, consultation, research and design services relating to automated systems and instruments for direct electrical detection and analysis of molecules for use in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defence; industrial analysis and research services in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defence; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services, namely, biological, chemical, biochemical, bacteriological DNA, RNA, protein, polynucleic acid and molecular analysis for scientific or research purposes; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services for chemical, biochemical, biological, bacteriological, molecular analysis, medical or veterinary purposes; DNA and polynucleic acid testing services for scientific or research purposes; information and advice regarding all the aforesaid services

Abstract

The invention resides in a microfluidic device for analysing a test liquid. The device has a preparation portion between an inlet and an upstream portion of the device, wherein said upstream portion is configured with a sensing chamber for housing a well array and for receiving via the inlet a test liquid to be sensed, and said preparation portion is configured having: a reagent port configured to receive a reagent for interacting with a test liquid to be sensed within the upstream portion; and/or an actuator, operably configured to induce a test liquid from the inlet towards the upstream portion. Between the inlet and respective outlet lies a sole channel. The channel can extend parallel to the substrate, preferably along its entire length. The preparation portion can be configured in a first part, and the upstream portion can be configured in a second part, wherein said first part and second part are connectable such that a sample received and processed in the first part can be passed to the second part for analysis.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

Provided herein are methods of characterising a target polypeptide as it moves with respect to a nanopore. Also provided are related kits, systems and apparatuses for carrying out such methods.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Abstract

Provided herein is a method of concentrating a tethering complex in a region of an amphiphilic layer, such as a lipid membrane. Also provided herein are methods of assembling a tethering complex; methods of concentrating an analyte in the region of a detector; amphiphilic layers; and arrays and devices for use in the disclosed methods.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
• B81B 1/00 - Devices without movable or flexible elements, e.g. microcapillary devices
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

Provided herein is a method of sequencing a target double stranded nucleic acid. The method comprises contacting the double stranded nucleic acid with a reagent as described herein to form a construct and sequencing the construct using a single-molecule sequencing technique as described herein. Associated products and kits are further provided.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6869 - Methods for sequencing

Abstract

The invention relates to a method of forming a sensing device (100) for supporting a plurality of nanopores upon an array of wells (10). The method involves providing a substrate, said substrate (102) having a surface (104) having an array of electrodes (6) located thereon for connecting to or for configuring upon an electronic circuit. Separately, a well array structure (108) is provided, which has an array of walls (110) defining through-holes (112) for defining wells (10). The substrate (102) and the well array structure (108) are aligning said array of electrodes (6) to define, at least in part, a portion of the bases of respective wells (10) at the bottom of the through holes (112).

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

A method of preparing a nucleic acid construct for single molecule characterisation, comprising contacting a target polynucleotide with: a polynucleotide-guided effector protein, a guide polynucleotide; a transposase; and a transposable element comprising a modified polynucleotide, wherein the polynucleotide-guided effector protein directs said transposase to a region of interest within the target polynucleotide and the transposase inserts the transposable element into the polynucleotide, thereby producing a nucleic acid construct for single molecule characterisation.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

Disclosed herein are compositions and methods that involve inserting connector protein channels of bacteriophage DNA packaging motors into copolymeric membranes via liposome-polymer fusion, which can be used as nanopore sensors for biomedical applications such as high throughput protein sequencing or cancer diagnosis. For example, disclosed are compositions comprising a copolymeric membrane into which a connector protein channel of a bacteriophage packaging motor has been inserted.

IPC Classes  ?

• B01D 69/14 - Dynamic membranes
• B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
• B01D 71/74 - Natural macromolecular material or derivatives thereof
• B01D 71/80 - Block polymers
• C12Q 1/6869 - Methods for sequencing

Abstract

An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

IPC Classes  ?

• G01N 1/18 - Devices for withdrawing samples in the liquid or fluent state with provision for splitting samples into portions
• B01J 19/00 - Chemical, physical or physico-chemical processes in general; Their relevant apparatus
• G01N 33/487 - Physical analysis of biological material of liquid biological material
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• C12Q 1/6869 - Methods for sequencing
• G01N 33/573 - Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Abstract

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12Q 1/6844 - Nucleic acid amplification reactions
• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices

Goods & Services

(1) Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non­-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research; reagents for medical and veterinary research; nucleic acid amplification reagents for medical research use. (2) Reagents for use in automated test systems for medical or veterinary use; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants for medical or veterinary use; reagents for medical and veterinary diagnosis; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary, medical or forensic use; reagents for immunoassay analysers for medical or veterinary use; reagents for testing aqueous solutions for medical or veterinary use; reagents for testing for susceptibility to allergies; reagents for use in biological processing for medical or veterinary use; reagents for use in nucleic acid characterisation and nucleic acid sequencing for medical or veterinary use; reagents for protein identification and analysis for medical or veterinary use; reagents for formation of amphipathic membranes for medical or veterinary use; buffer solutions for medical or veterinary use; aqueous solvents for medical or veterinary use; non-aqueous solvents for medical or veterinary use; non-aqueous media for medical or veterinary use; surfactants for medical or veterinary use; silicone oils for medical or veterinary use; enzymes for medical or veterinary use; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes. (3) Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; portable laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; cartridges being laboratory and scientific apparatus for holding biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents being laboratory equipment other than for medical use; reaction units containing chemical or biological reagents, being laboratory equipment, other than for medical use; Nanopore sensing devices; parts and fittings for all the aforesaid goods. (4) Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; medical and veterinary apparatus using electrowetting technology; automated apparatus and instruments for the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, for medical and veterinary purposes; Nanopore sensing devices; apparatus and instruments for medical diagnosis; portable apparatus and instruments for medical diagnosis; parts and fittings for all the aforesaid goods. (1) Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business services; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services connected with the sale of data analysis products and services and data streaming products and services; retail services connected with the sale of electronic sensing and measuring equipment, apparatus and instruments; retail services connected with the sale of sequencing equipment, apparatus and instruments; retail services connected with the sale of data processing equipment, apparatus and instruments; retail services connected with the sale of scientific equipment, apparatus and instruments; retail services connected with the sale of surgical, medical and veterinary equipment, apparatus and instruments; retail services connected with the sale of reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail services connected with the sale of chemicals and enzymes; retail services connected with the sale of chemicals and enzymes for scientific, medical or veterinary purposes; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; information, advisory and consultancy services relating to the aforesaid. (2) Training courses, workshops regarding the use of scientific and medical apparatus, instruments and equipment in the field of DNA/RNA related diagnostics, DNA/RNA sequencing, medical testing and related diagnostics; information, advisory and consultancy services relating to the aforesaid. (3) Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; synthesising services of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific testing purposes; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of data, including scientific and medical research data; computerised analysis of data; overseeing, monitoring and managing scientific and medical research experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical research data; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid. (4) Medical and veterinary diagnostic services; medical and veterinary testing services; genetic testing for medical and veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynuclear acid and molecular testing and analysis services and DNA/RNA sequencing services for medical, veterinary, agricultural and aquacultural purposes; information, advisory and consultancy services relating to the aforesaid.

Goods & Services

(1) Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research. (2) Reagents for use in automated test systems; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants; reagents for medical and veterinary diagnosis and medical and veterinary research; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary, medical, medical research or forensic use; reagents for immunoassay analysers; reagents for testing aqueous solutions; reagents for testing for susceptibility to allergies; reagents for use in biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; non-aqueous media; surfactants; silicone oils; enzymes; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes. (3) Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; laboratory and data processing apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, medical diagnosis, measurement of ions; portable laboratory and data processing apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, medical diagnosis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents for scientific and scientific research use; reaction units containing chemical or biological reagents, being laboratory equipment other than for medical use; nanopore sensing devices; parts and fittings for all the aforesaid goods. (4) Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; medical and veterinary apparatus using electrowetting technology; apparatus and instruments for the preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, for medical purposes; nanopore sensing devices being medical apparatus and instruments; parts and fittings for all the aforesaid goods. (1) Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business services; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services; retail services connected with the sale of data analysis products and services and data streaming products and services; retail services connected with the sale of electronic sensing and measuring equipment, apparatus and instruments; retail services connected with the sale of sequencing equipment, apparatus and instruments; retail services connected with the sale of data processing equipment, apparatus and instruments; retail services connected with the sale of scientific equipment, apparatus and instruments; retail services connected with the sale of surgical, medical and veterinary equipment, apparatus and instruments; retail services connected with the sale of reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail services connected with the sale of chemicals and enzymes; retail services connected with the sale of chemicals and enzymes for scientific, medical or veterinary purposes; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail services connected with the sale of goods for use in a laboratory; information, advisory and consultancy services relating to the aforesaid. (2) Education services; training services; training courses, workshops classes, symposiums and seminars; training courses, workshops, classes, symposiums and seminars regarding DNA/RNA sequencing, data analysis and conducting scientific tests and experiments; training courses, workshops, classes, symposiums and seminars regarding the use of scientific and medical apparatus, instruments and equipment; arranging and conducting conferences; information, advisory and consultancy services relating to the aforesaid. (3) Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; research and development of new products for others in the nature of the synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of technical data, including scientific and medical data; computerised analysis of data (terms considered too vague by the international bureau - rule 13 (2) (b) of the common regulations); overseeing, monitoring and managing scientific and medical research experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical research data; design of scientific experiments; analysis of technical data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services for scientific research purposes; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services for scientific research purposes; genome sequencing services and the analysis and evaluation of data resulting therefrom; preparation of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid. (4) Medical and veterinary diagnostic services; medical and veterinary testing services; genetic testing for medical and veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular testing and analysis services and DNA/RNA sequencing services for medical, veterinary, agricultural and aquacultural purposes; information, advisory and consultancy services relating to the aforesaid.

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid for use in scientific tests and experiments; ribonucleic acid for use in scientific tests and experiments; peptide nucleic acids for use in scientific and medical research; locked nucleic acids for use in scientific and medical research; nucleic acids for use in scientific or medical research; oligonucleotides for use in scientific or medical research; oligosaccharides for use in scientific and medical research; proteins for use in scientific and medical research; peptides for use in scientific and medical research; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases and glycosylases all for use in analytical tests for scientific and scientific research purposes; nucleotides, pyrimidines, and purines all for scientific and scientific research purposes; amino acids for laboratory or research use; saccharides for use in analytical tests for scientific and scientific research purposes; reagents for biological processing; reagents for use in nucleic acid characterization and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life, other than pesticides; reagents for formation of amphipathic membranes; Buffer solutions used in analytical chemistry; aqueous solvents for industrial purposes; nonaqueous solvents for industrial purposes; isopropanol for industrial purposes; ethanol not used as a fuel; phenol; non-aqueous cell culture media for scientific and research use; Non-ionic surfactants as raw materials for use in the manufacture of industrial products, consumer and household products; silicone oils for use in analytical tests for scientific and research use; silicone fluids; Enzymes for scientific and research purposes; lytic enzymes, including lysozyme, lyticase and cellulose, for scientific and research purposes; proteases for scientific and research purposes; nucleases, including RNase, DNase and restriction enzymes, for scientific and research purposes; chaotropes, including guanidine-HCl and guanidinium thiocyanate, for scientific and research purposes; detergents for industrial and scientific use; Protein for use in analytical tests for scientific and research use; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research Reagents for use in automated test systems for medical purposes; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, nonaqueous solvents and surfactants, for medical purposes; reagents for medical and veterinary diagnosis and medical and veterinary research; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary and medical use; reagents for immunoassay analyzers for veterinary and medical use; reagents for testing aqueous solutions for veterinary and medical use; reagents for testing for susceptibility to allergies for veterinary and medical use; reagents for use in biological processing for veterinary and medical use; reagents for use in nucleic acid characterization and nucleic acid sequencing for veterinary and medical use; reagents for protein identification and analysis for veterinary and medical use; reagents for formation of amphipathic membranes for veterinary and medical use; buffer solutions for use in disease testing for medical purposes; aqueous solvents for use in tests for medical and veterinary purposes; non-aqueous solvents for use in tests for medical and veterinary purposes; non-aqueous media, namely, cell growth media for growing cells for medical and veterinary use; silicone oils for medical use; enzymes for medical purposes; proteins arrays for use in tests for medical or veterinary diagnosis purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes downloadable computer software for performing and controlling the analysis of data obtained from scientific tests and experiments; Recorded medical software for performing and controlling the analysis of data obtained from scientific tests and experiments; computer software for running an e-commerce platform, namely, software that allows users to perform electronic business transactions over the Internet; Downloadable software for use in analyzing, transmitting and receiving data via a global communications network; downloadable software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; downloadable computer operating software; downloadable operating system software; downloadable operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; micro-array chips, namely, plates, glass slides or chips having multi-well arrays that can be used in chemical analysis, biological analysis or patterning for scientific, laboratory or medical research use; electronic sensing apparatus for analyzing chemical, biochemical, biological, bacteriological and microbiological test samples for scientific testing purposes; apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical and electrochemical analysis, measurement of ions; portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical and electrochemical analysis, measurement of ions; Scientific apparatus and instruments for sequencing DNA, RNA, protein and polynucleic acid for scientific or research purposes; Scientific apparatus and instruments for measuring relative DNA, RNA and protein and parts and fittings therefor; diagnostic and testing apparatus, not for medical purposes, namely, laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; apparatus and instruments for use in laboratory analysis, namely, empty cartridges for test samples, namely, cartridges for use with concentrates and solutions in solid or liquid form samples for scientific or research testing use; Computer hardware and recorded software programs for operating medical imaging apparatus sold as a unit; Automated laboratory apparatus and instruments for extracting nucleic acid and DNA for test samples, not for medical purposes; Electronic data processing apparatus; apparatus, devices and instruments for identifying, measuring, analyzing, sequencing and synthesizing small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analyzing, sequencing and synthesizing small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment, namely, stand alone electronic displays for medical images; silicon chips; DNA chips; computer docking stations; Electronic apparatus for electropysiological measurement and testing of cells in the nature of chemical and biological reagents; reaction units containing chemical and biological reagents, namely, reaction systems for catalyst research; Devices for sensing pores of nanometer size for laboratory use and scientific testing and research use; parts and fittings for all the aforesaid goods Surgical, medical and veterinary apparatus and instruments for use in general surgery; Medical diagnostic apparatus for testing DNA and RNA; Medical diagnostic instruments for the analysis of body fluids, namely, electro wetting devices for medical and veterinary purposes; portable medical and veterinary apparatus for DNA, RNA, and molecular phylogenetic analysis; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes, namely, ultrasound diagnostic apparatus; medical diagnostic apparatus for testing DNA and RNA samples; diagnostic apparatus for medical or veterinary purposes, namely, portable ultrasound diagnostic apparatus; Apparatus for DNA and RNA testing for medical and veterinary purposes; portable blood testing apparatus for medical and veterinary purposes; Blood testing apparatus for medical purposes, namely, cartridges for blood samples; reaction units containing chemical reagents, namely, reaction systems for medical testing purposes; Devices for sensing pores of nanometer size for medical or veterinary purposes; parts and fittings for all the aforesaid goods; none of the foregoing for or related to dental devices or the treatment of facial pain or disorders Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; Business development services; Business marketing services; retail store services and online retail store services featuring software and computer operating software; online retail store services for pharmaceutical, veterinary and sanitary preparations and medical supplies; retail store services featuring data analysis products and services; retail store services featuring equipment, apparatus, and instruments for electronic sensing and measuring; retail store services featuring sequencing equipment, apparatus and instruments; retail store services featuring data processing equipment, apparatus and instruments; retail store services featuring scientific equipment, apparatus and instruments; retail store services featuring surgical, medical, and veterinary equipment, apparatus and instruments; retail store services featuring reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail store services featuring chemicals and enzymes; retail store services connected featuring chemicals and enzymes for scientific, medical or veterinary purposes; retail store services featuring deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail store services featuring laboratory equipment; information, advisory and consultancy services relating to the aforesaid Arranging and conducting training courses, workshops, classes, symposiums and seminars regarding the use of scientific and medical apparatus, instruments and equipment in the field of DNA and RNA related diagnostics, DNA and RNA sequencing, medical testing and related diagnostics; arranging and conducting conferences in the field of DNA and RNA related diagnostics, DNA/RNA sequencing, medical testing and related diagnostics; information, advisory and consultancy services relating to the aforesaid Scientific and technological services, namely, scientific research in the fields of field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defense; industrial analysis and research services, namely, industrial analysis and research services in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defense; Scientific research, namely, chemical, biochemical, biological, bacteriological, medical and veterinary research; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; Medical research services in the field of synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; Scientific research, namely, medical and veterinary research; Scientific and technological services, namely, synthesizing chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for research purposes; Scientific and technological services, namely, synthesizing chemical compounds and small molecules for research purposes; chemical compound and small molecule synthesizing services; DNA and RNA and polynucleic acid sequencing for scientific purposes; protein sequencing for scientific purposes; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; Laboratory testing services, namely, testing of chemicals; Genetic testing services for scientific research purposes, namely, DNA and polynucleic acid testing services; Structural and functional analysis of genomes, namely, genome sequencing for scientific purposes; Analysis and evaluation of scientific and medical data for research purposes; Computerized analysis of scientific research data; Medical and scientific research, namely, overseeing, monitoring and managing scientific and medical experiments and tests; Medical and scientific research, namely, overseeing, monitoring and managing the analysis and evaluation of scientific and medical data; Scientific and technological services, namely, design of scientific experiments in the field of drug development; Scientific and technological services, namely, analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and quality evaluation services to assure compliance with industry standards; Analysis and Evaluation services in the field of DNA and RNA and polynucleic acid and protein sequencing to assure compliance with industry standards; Genome sequencing services in the field of scientific research and the structural and functional analysis of genomes data resulting there from; Information services, namely, provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; Technical data analysis in the field of scientific research; electronic data storage services; Application service provider (ASP) services featuring computer software applications for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices for use in the analysis of scientific and medical data; information, advisory and consultancy services relating to the aforesaid Medical and veterinary diagnostic services; Medical and veterinary testing services relating to the diagnosis and treatment of disease; genetic testing for medical and veterinary purposes; DNA, RNA, protein, polynucleic acid, and molecular testing and analysis services for medical and veterinary purposes; information, advisory and consultancy services relating to the aforesaid

Goods & Services

(1) Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research. (2) Reagents for use in automated test systems for medical or veterinary purposes; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants; reagents for medical and veterinary diagnosis and medical and veterinary research; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary, medical, medical research or forensic use; reagents for immunoassay analysers; reagents for testing aqueous solutions for medical or veterinary purposes; reagents for testing for susceptibility to allergies; reagents for use in biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing for medical or veterinary purposes; reagents for protein identification and analysis for medical or veterinary purposes; reagents for formation of amphipathic membranes for medical or veterinary purposes; buffer solutions for medical or veterinary purposes; aqueous solvents for medical or veterinary purposes; non-aqueous solvents for medical or veterinary purposes; non-aqueous media for medical or veterinary purposes; surfactants for medical or veterinary purposes; silicone oils for medical or veterinary purposes; enzymes for medical or veterinary purposes; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes. (3) Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; portable laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents; reaction units containing chemical or biological reagents; Nanopore sensing devices; parts and fittings for all the aforesaid goods. (4) Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; electrowetting devices for medical and veterinary purposes; apparatus and instruments for automating the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; reaction units containing chemical reagents, for medical purposes; parts and fittings for all the aforesaid goods. (1) Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business services; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services connected with the sale of data analysis products and services and data streaming products and services; retail services connected with the sale of electronic sensing and measuring equipment, apparatus and instruments; retail services connected with the sale of sequencing equipment, apparatus and instruments; retail services connected with the sale of data processing equipment, apparatus and instruments; retail services connected with the sale of scientific equipment, apparatus and instruments; retail services connected with the sale of surgical, medical and veterinary equipment, apparatus and instruments; retail services connected with the sale of reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail services connected with the sale of chemicals and enzymes; retail services connected with the sale of chemicals and enzymes for scientific, medical or veterinary purposes; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail services connected with the sale of goods for use in a laboratory; information, advisory and consultancy services relating to the aforesaid. (2) Education services; training services; training courses, workshops, classes, symposiums and seminars; training courses, workshops, classes, symposiums and seminars regarding DNA/RNA sequencing, data analysis and conducting scientific tests and experiments; training courses, workshops, classes, symposiums and seminars regarding the use of scientific and medical apparatus, instruments and equipment; arranging and conducting conferences; information, advisory and consultancy services relating to the aforesaid. (3) Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; services relating to the synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of technical data, including scientific and medical data; computerised analysis of data; overseeing, monitoring and managing scientific and medical experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical data; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid. (4) Medical and veterinary diagnostic services; medical and veterinary testing services; genetic testing for medical and veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular testing and analysis services and DNA/RNA sequencing services for medical, veterinary, agricultural and aquacultural purposes; information, advisory and consultancy services relating to the aforesaid.

Abstract

Provided herein is a method of loading a motor protein onto a polynucleotide adapter. Also provided are polynucleotide adapters and kits comprising such adapters. The adapters find use in characterising analytes such as polynucleotides in methods in which the polynucleotide moves in respect of a nanopore.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• G01N 33/487 - Physical analysis of biological material of liquid biological material
• G01N 33/92 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol

Abstract

A biochemical sensing system senses interactions between molecular entities and nanopores using a sensor device comprising an array of sensor elements that support the nanopores. A switch arrangement selectively connects detection channels for amplifying sensed electrical signals to respective sensor elements. On the basis of an analysis of the amplified electrical signal output from the detection channels, detection of completion of interactions at sensor elements occurs. In response thereto, the switch arrangement is controlled to connect the detection channel connected to a sensor element at which completion of an interaction has been detected to a further sensor element.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

A method for detecting interactions between elements within one or more DNA molecules within a cell, wherein the elements are not adjacent in the primary DNA sequence, the method comprising: a) providing a cell in which elements within one or more DNA molecules that are in close proximity are cross-linked; b) simultaneously lysing the cell and mechanically fragmenting the DNA molecules within the cell; c) proximity ligating the one or more fragmented DNA molecules; d) reversing the crosslinks in the ligated DNA molecules; e) sequencing the ligated DNA molecules; and f) analysing the sequencing data to detect interactions between elements within the one or more DNA molecules within the cell.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

A modified portal protein of a bacteriophage DNA packaging motor, wherein the modified portal protein is capable of direct insertion into a membrane and wherein the portal protein is modified compared to the wild type portal protein such that one or more amino acid residues on the outer surface of the portal protein is substituted by one or more other amino acid residue, and/or wherein a one or more amino acid residue is inserted on the outer surface of the portal protein so as to alter the outer surface hydrophobicity of the modified portal protein compared to the wild type portal protein.

IPC Classes  ?

• C07K 14/005 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
• B82Y 15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid for use in scientific tests and experiments; ribonucleic acid for use in scientific tests and experiments; peptide nucleic acids for use in scientific and medical research; locked nucleic acids for use in scientific and medical research; nucleic acids for use in scientific or medical research; oligonucleotides for use in scientific or medical research; oligosaccharides for use in scientific and medical research; proteins for use in scientific and medical research; peptides for use in scientific and medical research; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases and glycosylases all for use in analytical tests for scientific and scientific research purposes; nucleotides, pyrimidines, and purines all for scientific and scientific research purposes; amino acids for laboratory or research use; saccharides for use in analytical tests for scientific and scientific research purposes; reagents for biological processing; reagents for use in nucleic acid characterization and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life, other than pesticides; reagents for formation of amphipathic membranes; Buffer solutions used in analytical chemistry; isopropanol for industrial purposes; ethanol not used as a fuel; phenol; non-aqueous cell culture media for scientific and research use; Non-ionic surfactants as raw materials for use in the manufacture of industrial products, consumer and household products; silicone oils for use in analytical tests for scientific and research use; silicone fluids; Enzymes for scientific and research purposes; lytic enzymes, including lysozyme, lyticase and cellulose, for scientific and research purposes; proteases for scientific and research purposes; nucleases, including RNase, DNase and restriction enzymes, for scientific and research purposes; chaotropes, including guanidine-HCl and guanidinium thiocyanate, for scientific and research purposes; detergents for industrial and scientific use, namely, detergents for use in manufacturing processes; Protein for use in analytical tests for scientific and research use; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research; reagents for medical and veterinary research use; none of the foregoing for or relating to custom antisera development of antibodies or animal blood products Reagents for use in automated test systems for medical purposes; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, nonaqueous solvents and surfactants, for medical purposes; reagents for medical diagnosis use; reagents for veterinary diagnosis purposes; reagents for medical or veterinary diagnosis purposes; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary and medical use; reagents for immunoassay analyzers for veterinary and medical use; reagents for testing aqueous solutions for veterinary and medical use; reagents for testing for susceptibility to allergies for veterinary and medical use; reagents for use in biological processing for veterinary and medical use; reagents for use in nucleic acid characterization and nucleic acid sequencing for veterinary and medical use; reagents for protein identification and analysis for veterinary and medical use; reagents for formation of amphipathic membranes for veterinary and medical use; buffer solutions for use in disease testing for medical purposes; aqueous solvents for use in tests for medical and veterinary purposes; non-aqueous solvents for use in tests for medical and veterinary purposes; non-aqueous media, namely, cell growth media for growing cells for medical and veterinary use; silicone oils for medical use; enzymes for medical purposes; proteins arrays for use in tests for medical or veterinary diagnosis purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes; none of the foregoing for or relating to custom antisera development of antibodies or animal blood products downloadable computer software for performing and controlling the analysis of data obtained from scientific tests and experiments; Recorded medical software for performing and controlling the analysis of data obtained from scientific tests and experiments; downloadable computer software for running an e commerce platform, namely, software that allows users to perform electronic business transactions over the Internet; Downloadable software for use in analyzing, transmitting and receiving data via a global communications network; downloadable software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; downloadable computer operating software; downloadable operating system software; downloadable operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; micro-array chips, namely, plates, glass slides or chips having multi-well arrays that can be used in chemical analysis, biological analysis or patterning for scientific, laboratory or medical research use; electronic sensing apparatus for analyzing chemical, biochemical, biological, bacteriological and microbiological test samples for scientific testing purposes; laboratory apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical and electrochemical analysis, and measurement of ions, all for use in the science and research industry; portable laboratory apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical and electrochemical analysis, and measurement of ions, all for use in the science and research industry; Scientific apparatus and instruments for sequencing DNA, RNA, protein and polynucleic acid for scientific or research purposes; Scientific apparatus and instruments for measuring relative DNA, RNA and protein and parts and fittings therefor; diagnostic and testing apparatus, not for medical purposes, namely, laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; apparatus and instruments for use in laboratory analysis, namely, empty cartridges for test samples, namely, cartridges for use with concentrates and solutions in solid or liquid form samples for scientific or research testing use; Computer hardware and recorded software programs for operating medical imaging apparatus sold as a unit; Automated laboratory apparatus and instruments for extracting nucleic acid and DNA for test samples, not for medical purposes; Electronic data processing apparatus; laboratory apparatus, devices and instruments for identifying, measuring, analyzing, sequencing and synthesizing small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides, all for use in the science and research industry; portable laboratory apparatus, devices and instruments for identifying, measuring, analyzing, sequencing and synthesizing small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides, all for use in the science and research industry; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment, namely, stand alone electronic displays for medical images; silicon chips; DNA chips; batteries; rechargeable batteries; chargers for batteries; computer docking stations; apparatus for connecting and charging portable digital electronic devices, namely, battery charging devices; Electronic apparatus for electropysiological measurement and testing of cells in the nature of chemical and biological reagents; reaction units containing chemical and biological reagents, namely, reaction systems for catalyst research; Devices for sensing pores of nanometer size for laboratory use and scientific testing and research use; replacement and structural parts and fittings for all the aforesaid goods Surgical, medical and veterinary apparatus and instruments for use in general surgery; Medical diagnostic apparatus for testing DNA and RNA; Medical diagnostic instruments for the analysis of body fluids, namely, electro wetting devices for medical and veterinary purposes; portable medical and veterinary apparatus for DNA, RNA, and molecular phylogenetic analysis; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes, namely, ultrasound diagnostic apparatus; medical diagnostic apparatus for testing DNA and RNA samples; diagnostic apparatus for medical or veterinary purposes, namely, portable ultrasound diagnostic apparatus; Apparatus for DNA and RNA testing for medical and veterinary purposes; portable blood testing apparatus for medical and veterinary purposes; Blood testing apparatus for medical purposes, namely, cartridges for blood samples; Reaction units containing chemical reagents, namely, reaction systems for medical testing purposes comprised of medical diagnostic apparatus for testing for viruses and containing chemical reagents; Devices for sensing pores of nanometer size for medical or veterinary purposes; Medical diagnostic apparatus for testing DNA; Portable medical diagnostic apparatus for testing DNA; replacement and structural parts and fittings for all the aforesaid goods Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; Business development services; Business marketing services; retail store services and online retail store services featuring software and computer operating software; online retail store services for pharmaceutical, veterinary and sanitary preparations and medical supplies; retail store services featuring data analysis products and services; retail store services featuring equipment, apparatus, and instruments for electronic sensing and measuring; retail store services featuring sequencing equipment, apparatus and instruments; retail store services featuring data processing equipment, apparatus and instruments; retail store services featuring scientific equipment, apparatus and instruments; retail store services featuring surgical, medical, and veterinary equipment, apparatus and instruments; retail store services featuring reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail store services featuring chemicals and enzymes; retail store services connected featuring chemicals and enzymes for scientific, medical or veterinary purposes; retail store services featuring deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail store services featuring goods for use in a laboratory; information, advisory and consultancy services relating to the aforesaid Scientific and technological services, namely, scientific research in the fields of field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defense; industrial analysis and research services, namely, industrial analysis and research services in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defense; Scientific research, namely, chemical, biochemical, biological, bacteriological, medical and veterinary research; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services, all for scientific research purposes; Medical research services in the field of synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; Scientific research, namely, medical and veterinary research; Scientific and technological services, namely, synthesizing chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for research purposes; Scientific and technological services, namely, synthesizing chemical compounds and small molecules for research purposes; chemical compound and small molecule synthesizing services; DNA and RNA and polynucleic acid sequencing for scientific purposes; protein sequencing for scientific purposes; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; Laboratory testing services, namely, testing of chemicals; Genetic testing services for scientific research purposes, namely, DNA and polynucleic acid testing services; Structural and functional analysis of genomes, namely, genome sequencing for scientific purposes; Analysis and evaluation of scientific and medical data for research purposes; Computerized analysis of scientific research data; Medical and scientific research, namely, overseeing, monitoring and managing scientific and medical experiments and tests; Medical and scientific research, namely, overseeing, monitoring and managing the analysis and evaluation of scientific and medical data; Scientific and technological services, namely, design of scientific experiments in the field of drug development; Scientific and technological services, namely, analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and quality evaluation services to assure compliance with industry standards; Analysis and Evaluation services in the field of DNA and RNA and polynucleic acid and protein sequencing to assure compliance with industry standards; Genome sequencing services in the field of scientific research and the structural and functional analysis of genomes data resulting there from; Information services, namely, provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; Technical data analysis in the field of scientific research; electronic data storage services; Application service provider (ASP) services featuring computer software applications for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices for use in the analysis of scientific and medical data; information, advisory and consultancy services relating to the aforesaid; none of the foregoing for or relating to custom antisera development of antibodies or animal blood products Medical and veterinary diagnostic services; Medical and veterinary testing services relating to the diagnosis and treatment of disease; genetic testing for medical and veterinary purposes; DNA, RNA, protein, polynucleic acid, and molecular testing and analysis services for medical and veterinary purposes; DNA analysis services, namely, DNA screening for medical purposes; information, advisory and consultancy services relating to the aforesaid

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids for scientific or industrial purposes; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research. Reagents for use in automated test systems for medical or veterinary purposes; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants for medical or veterinary purposes; reagents for medical and veterinary diagnosis and medical and veterinary research; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary, medical, medical research or forensic use; reagents for immunoassay analysers for medical or veterinary purposes; reagents for testing aqueous solutions for medical or veterinary purposes; reagents for testing for susceptibility to allergies for medical or veterinary purposes; reagents for use in biological processing for medical or veterinary purposes; reagents for use in nucleic acid characterisation and nucleic acid sequencing for medical or veterinary purposes; reagents for protein identification and analysis for medical or veterinary purposes; reagents for formation of amphipathic membranes for medical or veterinary purposes; buffer solutions for medical or veterinary purposes; aqueous solvents for medical or veterinary purposes; non-aqueous solvents for medical or veterinary purposes; non-aqueous media; surfactants for medical or veterinary purposes; silicone oils for medical or veterinary purposes; enzymes for medical or veterinary purposes; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes. Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, medical diagnosis, measurement of ions; portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, medical diagnosis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents; reaction units containing chemical or biological reagents; Nanopore sensing devices; parts and fittings for all the aforesaid goods. Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; electrowetting devices for medical and veterinary purposes; apparatus and instruments for automating the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; reaction units containing chemical reagents, for medical purposes; Nanopore sensing devices; parts and fittings for all the aforesaid goods. Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business services; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services connected with the sale of data analysis products and services and data streaming products and services; retail services connected with the sale of electronic sensing and measuring equipment, apparatus and instruments; retail services connected with the sale of sequencing equipment, apparatus and instruments; retail services connected with the sale of data processing equipment, apparatus and instruments; retail services connected with the sale of scientific equipment, apparatus and instruments; retail services connected with the sale of surgical, medical and veterinary equipment, apparatus and instruments; retail services connected with the sale of reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail services connected with the sale of chemicals and enzymes; retail services connected with the sale of chemicals and enzymes for scientific, medical or veterinary purposes; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail services connected with the sale of goods for use in a laboratory; information, advisory and consultancy services relating to the aforesaid. Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; services relating to the synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; Analysis and evaluation of data, including scientific and medical data; computerised analysis of data; overseeing, monitoring and managing scientific and medical experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical data; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid. Medical and veterinary diagnostic services; medical and veterinary testing services; genetic testing for medical and veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular testing and analysis services for medical, veterinary, agricultural and aquacultural purposes; information, advisory and consultancy services relating to the aforesaid.

Abstract

Methods and apparatus for measuring current are provided. In one arrangement, a first charge amplifier integrates a current to be measured. A processing circuit filters an output from the first charge amplifier using a first low pass filter module and a second low pass filter module. A second charge amplifier integrates a current derived from the filtered output from the first charge amplifier. The apparatus is configured to reset the first charge amplifier at the start of each of a plurality of sensing frames. The processing circuit obtains at least a first sample of the output from the first charge amplifier in each sensing frame. The sampling of the f ir st sample alternates from one sensing frame to the next sensing frame between sampling via the first low pass filter module and sampling via the second low pass filter module.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• G01R 19/00 - Arrangements for measuring currents or voltages or for indicating presence or sign thereof

Abstract

The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/6869 - Methods for sequencing
• C07K 14/245 - Escherichia (G)
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

A nanopore sensing device has a structure (100) arranged to separate an analyte reservoir (106) and an outlet chamber (108). The structure (100) includes an array of nanopore structures (104), each nanopore structure (104) comprising a passage (114) for fluid connection through the structure between the analyte reservoir (106) and outlet chamber (108). Control terminals (156) are arranged for applying a control signal to alter the electrical potential difference across that nanopore structure (104). An electronic circuit (152) is configured to detect a signal from an electrical transduction element (126) at each nanopore structure (104).

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

A kit which has a first and second component parts, which are adapted for connection with each other. The first component part has a first array of electrical sensors, two substantially parallel lateral walls on the sides of the electrical connectors, two rails extending along the sides of the array, a front contact point and an overhang for receiving the second part. The second component part has a second array of electrical sensors for connection to the first array, front end configured to fit between the lateral walls of the first connector, and lateral sides having rail reliefs to fit the rails of the first connector. Connection of the first component part and the second component part forms a shoulder that aligns to locate the second array of electrical connectors in correct position for connection to the first array of electrical connectors.

IPC Classes  ?

• H01R 13/24 - Contacts for co-operating by abutting resiliently mounted
• H01R 13/187 - Pins, blades or sockets having separate spring member for producing or increasing contact pressure the spring member being in the socket

Abstract

The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C07K 14/35 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
• C12Q 1/6869 - Methods for sequencing

Abstract

Provided herein are methods of encoding data on a polymer. Also provided are methods of reading data encoded on a polymer. Also provided are systems for encoding data on a polymer; systems for reading data encoded on a polymer; and data encoding / data reading platforms.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• C12Q 1/6869 - Methods for sequencing
• G16B 30/20 - Sequence assembly
• B82Y 10/00 - Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• G11C 13/00 - Digital stores characterised by the use of storage elements not covered by groups , , or

Abstract

Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3′ and/or 5′ ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3′ and/or 5′ end sequences; and/or the flanking sequence.

IPC Classes  ?

• C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism
• C12Q 1/6869 - Methods for sequencing
• G16B 30/00 - ICT specially adapted for sequence analysis involving nucleotides or amino acids

Abstract

A signal derived from a polymer comprising a series of polymer units during translocation of the polymer with respect to a nanopore is analysed using a machine learning technique. A series of weight distributions is output, each comprising weights in respect of transitions between labels over a set of labels representing the possible types of polymer unit in the polymer. An estimate of the series of polymer units from the weight distributions. Use of weights in respect of the transitions increases the accuracy of the technique.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

The invention relates to a sensing system comprising an electrowetting device, which electrowetting device comprises an array of actuation electrodes, and a control system configures to perform droplet operations on a system of droplets present in the sensing system. The invention also relates to a method of operating the sensing system of the invention. The invention also provides novel droplet constructs which can be made and manipulated in the sensing system of the invention.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• A61K 9/50 - Microcapsules
• A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
• C12Q 1/6869 - Methods for sequencing

Abstract

A system for characterising a target polynucleotide, the system comprising a membrane and a pore complex; wherein the pore complex comprises: (i) a nanopore located in the membrane, and (ii) an auxiliary protein or peptide attached to the nanopore; wherein the nanopore and the auxiliary protein or peptide together form a continuous channel across the membrane, the channel comprising a first constriction region and a second constriction region; wherein the first constriction region is formed by a portion of the nanopore, and wherein the second constriction region is formed by at least a portion of the auxiliary protein or peptide.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research; reagents for medical and veterinary research; nucleic acid amplification reagents for medical research use. Reagents for use in automated test systems for medical or veterinary use; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants for medical or veterinary use; reagents for medical and veterinary diagnosis; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary, medical or forensic use; reagents for immunoassay analysers for medical or veterinary use; reagents for testing aqueous solutions for medical or veterinary use; reagents for testing for susceptibility to allergies; reagents for use in biological processing for medical or veterinary use; reagents for use in nucleic acid characterisation and nucleic acid sequencing for medical or veterinary use; reagents for protein identification and analysis for medical or veterinary use; reagents for formation of amphipathic membranes for medical or veterinary use; buffer solutions for medical or veterinary use; aqueous solvents for medical or veterinary use; non-aqueous solvents for medical or veterinary use; non-aqueous media for medical or veterinary use; surfactants for medical or veterinary use; silicone oils for medical or veterinary use; enzymes for medical or veterinary use; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes. Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; portable laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; cartridges being laboratory and scientific apparatus for holding biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents being laboratory equipment other than for medical use; reaction units containing chemical or biological reagents, being laboratory equipment, other than for medical use; Nanopore sensing devices; parts and fittings for all the aforesaid goods. Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; medical and veterinary apparatus using electrowetting technology; automated apparatus and instruments for the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, for medical and veterinary purposes; Nanopore sensing devices; apparatus and instruments for medical diagnosis; portable apparatus and instruments for medical diagnosis; parts and fittings for all the aforesaid goods. Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business services; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services; retail services connected with the sale of data analysis products and services and data streaming products and services; retail services connected with the sale of electronic sensing and measuring equipment, apparatus and instruments; retail services connected with the sale of sequencing equipment, apparatus and instruments; retail services connected with the sale of data processing equipment, apparatus and instruments; retail services connected with the sale of scientific equipment, apparatus and instruments; retail services connected with the sale of surgical, medical and veterinary equipment, apparatus and instruments; retail services connected with the sale of reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail services connected with the sale of chemicals and enzymes; retail services connected with the sale of chemicals and enzymes for scientific, medical or veterinary purposes; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail services connected with the sale of goods for use in a laboratory; information, advisory and consultancy services relating to the aforesaid. Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; synthesising services of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific testing purposes; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of data, including scientific and medical research data; computerised analysis of data; overseeing, monitoring and managing scientific and medical research experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical research data; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid.

Abstract

The invention resides in a method of determining a sequence of a target polymer, or part thereof, comprising polymer units comprising canonical and non-canonical polymer units. The method comprises taking a series of measurements of a signal relating to the target polymer wherein a measurement of the signal is dependent upon a plurality of polymer units, and wherein the polymer units of the target polymer modulate the signal, and wherein a non- canonical polymer unit modulates the signal differently from a corresponding canonical polymer unit. The series of measurements are analysed using a machine learning technique that attributes a measurement of a non-canonical polymer unit to being a measurement of a respective corresponding canonical polymer unit. The sequence of the target polymer, or part thereof, is determined from the analysed series of measurements. A non-canonical polymer unit identified from the analysis can be additionally or alternatively determined. Two or more types of non-canonical polymer units corresponding to the two or more types of canonical polymer unit can be used. The polynucleotide can be DNA.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing

Abstract

The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• C12Q 1/6869 - Methods for sequencing
• C12N 9/14 - Hydrolases (3.)
• G01N 27/447 - Systems using electrophoresis

Abstract

The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• G01N 27/447 - Systems using electrophoresis
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• C12Q 1/6844 - Nucleic acid amplification reactions

Abstract

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.

IPC Classes  ?

• C12Q 1/6844 - Nucleic acid amplification reactions
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.

IPC Classes  ?

• C12Q 1/6844 - Nucleic acid amplification reactions
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.

IPC Classes  ?

• C07K 14/425 - Zeins
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C07K 14/245 - Escherichia (G)
• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• G01N 27/447 - Systems using electrophoresis

Abstract

The invention relates to a new method of characterising two or more target polynucleotides using a pore. The method involves sequentially attaching to a first polynucleotide one or more subsequent polynucleotides to form a concatenated polynucleotide.

IPC Classes  ?

• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• B82Y 15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• G01N 33/487 - Physical analysis of biological material of liquid biological material
• G01N 27/447 - Systems using electrophoresis

Abstract

Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing

Abstract

A component (8) adapted to engage with a receiver (6) has an array of contact pads (16) to removably connect with a corresponding array of connectors (18) on the receiver (6). Each contact pad (16) of the array is electrically connected to the electrode (26) of a corresponding recess or well (28) that is part of a sensor, wherein a membrane is formable across each recess. A conductive grid (102) is configured between the contact pads (16) of the array, to inhibit an electrostatic discharge (BSD) conducting across the recesses or wells and/or direct an BSD away from the recesses or wells.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

The invention provides methods of selecting polynucleotides based on their size in globular form. The polynucleotides may be treated with a condensation medium, e.g. comprising polyethylene glycol, e.g. PEG8000, and salt, in order to adopt a globular form. The method may employing filtering in order to retain the larger nucleic acids and make the larger nucleic acids available for sequencing applications, such as nanopore sequencing. The invention also relates to kits for selecting polynucleotides.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C40B 40/08 - Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Abstract

A method of attaching an adapter to a polynucleotide comprising: providing a double stranded polynucleotide comprising a single stranded break point within its polynucleotide sequence; contacting said double stranded polynucleotide with an exonuclease to form a single stranded region initiated at said break point; attaching an adapter to said single stranded region.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6855 - Ligating adaptors

Abstract

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12Q 1/6855 - Ligating adaptors

Abstract

A method for selectively modifying a target polynucleotide in a sample of polynucleotides, the method comprising contacting a sample of polynucleotides with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein such that the polynucleotide-guided effector protein cuts the target polynucleotide to produce a cut end comprising an overhang; and attaching an adapter to the cut end in the target polynucleotide.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6869 - Methods for sequencing

Abstract

The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• B01J 19/00 - Chemical, physical or physico-chemical processes in general; Their relevant apparatus
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

A method of amplifying a target polynucleotide, comprising: providing a template polynucleotide comprising a 5' hairpin, a target polynucleotide and a 3' hairpin, wherein the 5' hairpin comprises one or more non-canonical nucleotides; and contacting the template polynucleotide with a polymerase and canonical nucleotides, wherein the polymerase extends, using the canonical nucleotides, the target polynucleotide from its 3' end to form a first extended polynucleotide comprising the 5' hairpin at its 5' end and the complement of the 5' hairpin at its 3' end, wherein the complement of the 5' hairpin forms a 3' hairpin; and the polymerase extends the first extended polynucleotide from its 3' end to form a second extended polynucleotide comprising the 5' hairpin at its 5' end and the complement of the 5' hairpin at its 3' end, wherein the complement of the 5' hairpin forms a 3' hairpin.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
• C12Q 1/6869 - Methods for sequencing

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems not for medical purposes; reagents for analytical tests not for medical purposes; reagents for industrial use, namely, for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid for laboratory use and scientific testing and research use; ribonucleic acid for laboratory use and scientific testing and research use; peptide nucleic acids for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics; locked nucleic acids for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics; nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides all for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases for use in analytical tests for scientific and scientific research purposes; nucleotides, pyrimidines, purines; amino acids for use in analytical tests for scientific and scientific research purposes; saccharides for use in analytical tests for scientific and scientific research purposes; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life other than pesticides; reagents for formation of amphipathic membranes; chemicals, namely, buffer solutions for scientific and research use; isopropanol; ethanol; phenol for scientific and research use; surfactants for scientific and research use; silicone oils for scientific and research use; silicone fluids; enzymes for scientific and research use; lytic enzymes including lysozyme, lyticase and cellulose for scientific and research use; proteases; nucleases, including RNase, DNase and restriction enzymes for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics; chaotropes, including guanidine-HCl and guanidinium thiocyanate for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics; detergents for industrial and scientific use; proteins for use in analytical tests for scientific and research use; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research Reagents for use in automated test systems for medical and veterinary use; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants, all for medical and veterinary use; reagents for medical and veterinary diagnosis; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary and medical purposes; reagents for medical or veterinary use, namely, reagents for immunoassay analysers; reagents for medical or veterinary use, namely, reagents for testing aqueous solutions; reagents for medical or veterinary use, namely, for testing for susceptibility to allergies; reagents for medical or veterinary use, namely, for use in biological processing; reagents for medical or veterinary use, namely, reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis for medical or veterinary use; reagents for medical or veterinary use, namely, reagents for formation of amphipathic membranes; chemicals, namely, buffer solutions for medical or veterinary use; surfactants for use in medical and veterinary diagnostic tests; enzymes for use in tests for medical and veterinary purposes; protein arrays for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes Downloadable computer software for running an e-commerce platform; downloadable software for use in analysing, transmitting and receiving data via a global communications network; downloadable software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; downloadable computer operating software; downloadable computer operating system software; downloadable operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; laboratory equipment, namely, micro-array chips; electronic sensing apparatus for analysing chemical, biochemical, biological, bacteriological and microbiological test samples for scientific testing purposes; apparatus and instruments, namely, optical or electrochemical readers for the analysis of biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples and the measurement of ions; portable apparatus and instruments, namely, optical or electrochemical readers for the analysis of biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples for and the measurement of ions; portable optical or electrochemical readers for the analysis of biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples for medical diagnostic purposes; scientific instruments, namely, sequencing devices for use in DNA, RNA, protein and polynucleic acid sequencing; downloadable computer software and optical and electrochemical readers for the scientific analysis of polynucleic acid sequences; downloadable computer software and optical and electrochemical readers for the analysis of nucleic and polynucleic acids; scientific apparatus and instruments for the analysis of proteins; apparatus and instruments for the analysis of genetic information, genomic information and proteomic information, namely, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICS (application specific integrated circuits), and networked computer hardware and downloadable software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis; downloadable computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific research and scientific testing purposes; data processing apparatus, devices and instruments; scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific research and scientific testing purposes; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; electronic display interfaces for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips for scientific or research use; biosensor chips for scientific or research use; cartridges being laboratory and scientific apparatus for holding biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples; batteries; rechargeable batteries; chargers for batteries; power packs in the nature of batteries; computer docking stations; apparatus for connecting and charging portable digital electronic devices; Nanopore sensing devices for laboratory use and scientific testing and research use; parts and fittings for all the aforesaid goods Medical apparatus and instruments for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; veterinary apparatus and instruments for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; portable medical and veterinary apparatus for diagnosing genetic disorders for medical purposes; blood testing apparatus; genetic disorder diagnostic apparatus for medical or veterinary purposes; portable genetic disorder diagnostic apparatus for medical or veterinary purposes; immunochemical testing apparatus for medical or veterinary purposes; portable immunochemical testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; reaction units containing chemical reagents, for medical purposes; nanopore sensing devices for medical or veterinary purposes; parts and fittings for all the aforesaid goods Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business information services; analysis and evaluation of business information; retail store services featuring software; retail store services featuring computer operating software; retail store services featuring data analysis products and services and data streaming products and services; retail store services featuring electronic sensing and measuring equipment, apparatus and instruments; retail store services featuring sequencing equipment, apparatus and instruments; retail store services featuring data processing equipment, apparatus and instruments; retail store services featuring scientific equipment, apparatus and instruments; retail store services featuring surgical, medical and veterinary equipment, apparatus and instruments; retail store services featuring reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail store services featuring chemicals and enzymes; retail store services featuring chemicals and enzymes for scientific, medical or veterinary purposes; retail store featuring deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail store services featuring goods for laboratory use; information, advisory and consultancy services relating to the aforesaid Scientific and technological services and research and design relating thereto, namely, consultation, research and design services relating to automated systems and instruments for direct electrical detection and analysis of molecules for use in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defence; industrial analysis and research services in the fields of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defence; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services, namely, biological, chemical, biochemical, bacteriological DNA, RNA, protein, polynucleic acid and molecular analysis for scientific or research purposes; scientific and technological services, namely, synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; scientific research for medical or veterinary purposes; DNA and RNA and polynucleic acid sequencing services for scientific research purposes; protein sequencing services for scientific research purposes; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; DNA and polynucleic acid testing services, namely, the analysis of DNA and polynucleic acids for scientific and scientific research purposes; genome sequencing services for scientific testing and scientific research purposes; analysis and evaluation of data, not for medical treatment or diagnostic purposes, namely, scientific and medical data; computerised analysis of technical data namely, the results of scientific tests and experiments; overseeing, monitoring and managing scientific and medical experiments and tests for scientific research purposes; overseeing, monitoring and managing the analysis and evaluation of scientific and medical data for scientific research purposes; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests for scientific research purposes; scientific and technological services, namely, biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services; scientific and technological services, namely DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services; scientific and technological services, namely, genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests none being for medical treatment or diagnostic purposes; scientific and medical research and development services in the fields of scientific, technological and medical research; technical data analysis and evaluation services in the fields of scientific, medical technology and medical research; electronic data storage services; application service provider (ASP) services featuring computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices for the analysis of scientific data; information, advisory and consultancy services relating to the aforesaid

Goods & Services

(1) Chemical diagnostic reagents for use in scientific tests and experiments for scientific research purposes, chemical diagnostic reagents for use in automated test systems for medical and veterinary testing purposes and for use in scientific tests and experiments for scientific research purposes, reagents for analytical tests on samples for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, reagents for industrial use, namely, synthesising DNA, RNA and other nucleic acids, reagents for use in scientific tests, namely, the sequencing of DNA, RNA and other nucleic acids, reagents for laboratory tests for scientific research purposes, reagents for use in testing and analysing test samples in the fields of molecular biology, biotechnology, microbiology, and bacteriology, reagents for scientific purposes for use in nucleic acid amplification, reagents for use in forensic science, reagents for blood grouping, other than for medical and veterinary purposes, reagents used for analytical moisture determinations, reagents for testing water and biological fluids, namely, blood, saliva, urine, interstitial fluid, serum, plasma, semen, tears, bile fluid, spinal fluid, and amniotic fluid, reagents for testing aqueous media for use in medical and veterinary tests for the purposes of identifying and diagnosing medical conditions, diseases and disorders, deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid for laboratory use and scientific testing and research use, ribonucleic acid for laboratory use and scientific testing and research use, peptide nucleic acids for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics, locked nucleic acids for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides all for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics, oligonucleotides for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, oligosaccharides for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, proteins for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, peptides for industrial purposes, scientific purposes and scientific research purposes, chemical reagents for genetic identity testing, endonucleases, polymerases, ligases, glycosylases, all for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, nucleotides, pyrimidines, purines for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, amino acids for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, saccharides for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, reagents for scientific purposes for use in nucleic acid characterisation and nucleic acid sequencing, reagents for scientific purposes for protein identification and analysis, chemical preparations for detecting pathogenic infections in plants, reagents for formation of amphipathic membranes for scientific use, buffer solutions for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, aqueous solvents for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, non-aqueous solvents for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, isopropanol for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, ethanol, phenol, non-aqueous media for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, surfactants for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, silicone oils, silicone fluids, enzymes for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, lytic enzymes namely lysozyme, lyticase and cellulose for scientific and research use, proteases enzymes for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics, nucleases, namely RNase, DNase and restriction enzymes for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics, chaotropes agents, namely, guanidine-HCl and guanidinium thiocyanate for use in scientific tests and experiments for scientific research purposes, chaotropic agents, namely, guanidine-HCI and guanidinium thiocyanate for use in diagnostic tests for medical and veterinary purposes, detergents for industrial and scientific use, proteins for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, all the aforesaid other than for medical and veterinary purposes; unprocessed synthetic resin, plastic and silicone compounds in the form of microspheres used to incorporate various other substances; nucleic acid amplification reagents for medical research use. (2) Diagnostic chemical reagents for use in automated test systems for medical and veterinary use; chemical and diagnostic reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, namely reagents for formation of amphipathic membranes, buffer solutions, nonaqueous solvents, surfactants for medical and veterinary use; diagnostic reagents for medical and veterinary use; diagnostic reagents for laboratory tests for medical and veterinary purposes; nucleic acid amplification reagents for veterinary, medical and forensic use; diagnostic reagents for immunoassay analysers for medical and veterinary use; diagnostic test reagents for testing aqueous solutions for medical and veterinary use; diagnostic test reagents for susceptibility to allergies for medical and veterinary use; diagnostic reagents for use in biological processing for medical and veterinary use; reagents for use in nucleic acid characterisation and nucleic acid sequencing for medical and veterinary use; chemical reagents for protein identification and analysis for medical and veterinary use; chemical reagents for formation of amphipathic membranes for medical and veterinary use; surfactants for use in medical and veterinary diagnostic tests; proteins for use in diagnostic tests for medical and veterinary purposes; magnetic and non-magnetic albumin microspheres made from rubber, plastics or silicone, namely, beads that facilitate delivery of a wide range of pharmaceuticals. (3) Computer software for the analysis of scientific and medical data in the fields of genomics, protein analysis, molecular analysis, life sciences, health care, national security and military defence obtained from scientific tests and experiments; computer operating software; operating system software; biochip sensors; micro-array chips; laboratory apparatus and scientific instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, namely, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICs (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis for scientific and research purposes; scientific apparatus and instruments for sequencing DNA, RNA, proteins and polynucleic acids; scientific apparatus and laboratory devices for the scientific analysis of polynucleic acid sequences; apparatus and instruments for analysis of genetic information, genomic information and proteomic information, namely, automated instruments for detecting the presence, properties and identity of analytes comprising of cartridges, microarrays, silicon chip based arrays, ASICS (application specific integrated circuits) and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis; apparatus and instruments for use in laboratory analysis, namely, empty cartridges for test samples, namely, cartridges for use with concentrates and solutions in solid and liquid form samples for scientific and research testing; computer programs, computer hardware and computer software for database management; electrowetting devices, namely electrodes for laboratory research, electrowetting on dielectric (EWOD) digital microfluidic devices consisting of an array of metal electrodes covered with a continuous hydrophobic dielectric layer; scientific apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides for scientific research and scientific testing purposes; computer hardware for data processing; scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing and synthesising small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides for scientific research and scientific testing purposes; portable scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing and synthesising small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides for scientific research and scientific testing purposes; portable computer hardware and stationary data processing apparatus for analysis and recordal of genetic information, genomic information and proteomic information, namely, automated instruments for detecting the presence, properties and identity of analytes comprising of cartridges, microarrays, silicon chip based arrays, ASICS (application specific integrated circuits) and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis; electronic display interfaces, namely, monitors for the display of information obtained from scientific equipment; silicon chips; DNA chips; molecular analyte detector chips; DNA biosensor chips; laboratory and scientific cartridges for holding biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples in solid and liquid form for scientific and research testing; computer docking stations; nanopore sensors. (4) Diagnostic and analytical apparatus for medical and veterinary purposes, namely, automated electronic devices for sequencing DNA, RNA and other nucleic acids for the purposes of detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis for the purposes of detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases and conditions; blood testing apparatus for chemical, biochemical and biological analysis of blood samples; nanopore sensors for medical and veterinary purposes. (1) Analysis and evaluation of business information in the fields of genomics, protein analysis, molecular analysis, chemistry, biochemistry, biology, biotechnology, microbiology and bacteriology, medical research, medical testing and diagnosis, national security and military defence; provision of reports relating to business information in the fields of genomics, protein analysis, molecular analysis, chemistry, biochemistry, biology, biotechnology, microbiology and bacteriology, medical research, medical testing and diagnosis, national security and military defence; sale of computer software; sale of computer operating software; retail services in the field of strategic business analytic reports and marketing analysis services; retail sale of electronic sensing and measuring equipment, apparatus and instruments for the analysis and sequencing of DNA, RNA and other polynucleic acids for scientific research and testing purposes; retail sale services of genome, protein, DNA and RNA and polynucleic acid sequencers for scientific purposes; retail sale services of bioinformatics software, computer hardware and computer software for database management; retail sale services of chemicals and enzymes for use in scientific tests, namely, the sequencing of DNA, RNA and other nucleic acids; retail sale services of chemicals and enzymes for sequencing of DNA, RNA and other nucleic acids for scientific, medical and veterinary purposes; retail sale services of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides, all for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids. (2) Scientific and technological services and research and design relating thereto, namely, consultation, research and design services relating to automated systems and instruments for direct electrical detection and analysis of molecules for use in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, national security and military defence; industrial analysis and research services in the fields of genomics, protein analysis, molecular analysis, life sciences, healthcare, national security and military defence; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services, namely, biological, chemical, biochemical, bacteriological DNA, RNA, protein, polynucleic acid and molecular analysis for scientific and research purposes; synthesising services, namely natural and chemical creation of chemical compounds, small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides for scientific testing purposes; scientific research for chemical, biochemical, biological, bacteriological, medical and veterinary purposes; synthesising services of chemical compounds, small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides for scientific testing purposes; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; DNA and polynucleic acid testing services for scientific research purposes; genome sequencing services; scientific analysis and evaluation in the fields of genomics, protein analysis, molecular analysis, chemistry, biochemistry, biology, biotechnology, microbiology and bacteriology; overseeing, monitoring and managing genetic testing for scientific and medical research purposes; overseeing, monitoring and managing analyses and evaluations in the fields of genomics, protein analysis, molecular analysis, chemistry, biochemistry, biology, biotechnology, microbiology and bacteriology; developing programs for simulating experiments or series of experiments in a virtual optical laboratory; medical research; technical evaluation services in the fields of chemistry, biology, biotechnology, genomics, pharmaceutical development, medical research and nanotechnology; electronic storage of medical records; application service provider (ASP) services featuring application programming interface software for analysis of scientific and medical records obtained from sequencing DNA, RNA and other polynucleic acids and installed and downloaded onto medical image processors, computers, tablet computers and handheld mobile digital electronic devices.

Abstract

A pump for use in low-profile applications comprises a barrel for holding fluid; and a piston that converts a rotational driving force into a longitudinal driving motion within the barrel. The pump provides space saving advantages by reducing the need for external equipment and mechanisms around the pump for providing actuation or moving the actuating mechanism.

IPC Classes  ?

• A61M 5/315 - Pistons; Piston-rods; Guiding, blocking or restricting the movement of the rod; Appliances on the rod for facilitating dosing
• A61B 17/88 - Methods or means for implanting or extracting internal fixation devices
• F04B 13/00 - Pumps specially modified to deliver fixed or variable measured quantities
• F04B 7/06 - Piston machines or pumps characterised by having positively-driven valving in which the valving is performed by pistons and cylinders coacting to open and close intake or outlet ports the pistons and cylinders being relatively reciprocated and rotated
• F04B 9/02 - Piston machines or pumps characterised by the driving or driven means to or from their working members the means being mechanical
• A61M 5/145 - Pressure infusion, e.g. using pumps using pressurised reservoirs, e.g. by means of pistons
• F04B 17/03 - Pumps characterised by combination with, or adaptation to, specific driving engines or motors driven by electric motors
• B29L 31/26 - Sealing devices, e.g. packaging for pistons or pipe joints
• F04B 53/14 - Pistons, piston-rods or piston-rod connections

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research. Reagents for use in automated test systems; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants; reagents for medical and veterinary diagnosis and medical and veterinary research; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary, medical, medical research or forensic use; reagents for immunoassay analysers; reagents for testing aqueous solutions; reagents for testing for susceptibility to allergies; reagents for use in biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; non-aqueous media; surfactants; silicone oils; enzymes; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes. Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; scientific testing and data processing apparatus and instruments, other than for medical use, for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; portable scientific testing and data processing apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting-on-dielectric (EWOD) devices; scientific apparatus and instruments for automating the preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; scientific apparatus, namely, cartridges for use with test samples for scientific testing and scientific research purposes; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents for scientific and scientific research use; nanopore sensing devices; parts and fittings for all the aforesaid goods. Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; electrowetting-on-dielectric (EWOD) devices for medical and veterinary purposes; automated apparatus and instruments for the preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, for medical and veterinary purposes; nanopore sensing devices for medical and veterinary purposes; apparatus and instruments for medical diagnosis; portable apparatus and instruments for medical diagnosis; parts and fittings for all the aforesaid goods. Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific testing purposes; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of data, including scientific and medical research data; computerised analysis of scientific and medical research data; overseeing, monitoring and managing scientific and medical research and diagnostic experiments; overseeing, monitoring and managing the analysis and evaluation of scientific and medical research data; design of scientific experiments; analysis of data generated by scientific and medical research experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services for scientific research and scientific testing purposes and for medical research; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services for scientific testing and scientific research purposes; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical research and diagnostic experiments, the results of scientific and medical research and diagnostic experiments, the analysis and evaluation of scientific and medical research data, and data generated by scientific and medical research and medical diagnostic experiments; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computerised analysis of medical research data; overseeing, monitoring and managing medical research tests; overseeing, monitoring and managing the analysis and evaluation of medical research data; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services for medical research; provision of reports relating to the results of medical research tests, the analysis and evaluation of medical research data and data generated by medical research tests; information, advisory and consultancy services relating to the aforesaid.

Abstract

Disclosed herein are compositions and methods that involve inserting connector protein channels of bacteriophage DNA packaging motors into copolymeric membranes via liposome-polymer fusion, which can be used as nanopore sensors for biomedical applications such as high throughput protein sequencing or cancer diagnosis. For example, disclosed are compositions comprising a copolymeric membrane into which a connector protein channel of a bacteriophage packaging motor has been inserted.

IPC Classes  ?

• B82Y 15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• C12Q 1/6869 - Methods for sequencing
• B01D 69/06 - Flat membranes

Abstract

The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• B82Y 15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• G01N 33/487 - Physical analysis of biological material of liquid biological material
• G01N 27/447 - Systems using electrophoresis

Abstract

in vitroin vitro methods for synthesising a polymer, particularly a polynucleotide molecule, having a pre-defined sequence of units such as nucleotides. For synthesising a polynucleotide molecule the methods involve a process of extending a polynucleotide synthesis molecule with a transfer nucleotide. The methods additionally involve repeating the extension process multiple times to iteratively extend the polynucleotide molecule with multiple transfer nucleotides to generate a new polynucleotide molecule having a pre-defined nucleotide sequence. The invention also relates to in vitro methods of joining multiple synthetic polynucleotides following synthesis to form larger synthetic polynucleotides, as well as devices and systems for performing the extension, synthesis and assembly methods of the invention.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/6844 - Nucleic acid amplification reactions

Abstract

A method for selecting polynucleotides, the method comprising: allowing a nucleic acid handling enzyme to move along multiple polynucleotides in a sample for a defined time period, wherein the enzyme is loaded onto each of the multiple polynucleotides and wherein one or more molecule of the enzyme moves along each of the multiple polynucleotides; and selecting polynucleotides based on whether or not the enzyme reaches the end of and/or unbinds from the polynucleotides in the defined time period.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• G01N 15/14 - Electro-optical investigation
• G01N 27/447 - Systems using electrophoresis
• C12Q 1/6869 - Methods for sequencing
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

A target polynucleotide is expanded. In respect of each nucleotide in the target polynucleotide, the target polynucleotide comprises clock nucleotides and at least one signal nucleotide in a predetermined order. The clock nucleotides have a predetermined sequence common to each nucleotide in the target polynucleotide. The at least one signal nucleotide is characteristic of the identity of the respective nucleotide in the target polynucleotide. During translocation of the expanded polynucleotide through a nanopore, electrical measurements dependent on the polynucleotide within the pore are made, to derive an analysis signal. Clock signals derived from the clock nucleotides are identified. Relative to the positions of the identified clock signals, nucleotide signals derived from the least one signal nucleotide are derived to analyse the target polynucleotide. The predetermined sequence of the clock nucleotides comprises a restriction site for a restriction enzyme and at least one further nucleotide that extends the predetermined sequence.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

A microfluidic device for analysing a test liquid comprises: a sensor (235), such as a membrane provided with nanopores, provided in a sensing chamber (237); a sensing chamber inlet channel (261) and a sensing chamber outlet channel (262), each connecting to the sensing chamber for respectively passing liquid into and out of the sensing chamber, and a reservoir (233) forming a sample input port to the microfluidic device, the reservoir being in fluid communication with the sensing chamber inlet channel (261); a liquid collection channel (232); a barrier (231) between an end of the sensing chamber outlet channel (262) and the liquid collection channel (232); a first seal (251), covering the sample input port; a second seal (252), covering the end of the sensing chamber outlet channel (262), thereby preventing liquid from flowing from the sensing chamber (237), over the barrier (231), into the liquid collection channel (232); wherein the microfluidic device is filled with a liquid from the first seal (251) at the sample input port to the second seal (252), such that the sensor (235) is covered by liquid and unexposed to a gas or gas/liquid interface; and wherein the first and second seals (251, 252) are removable to cause the liquid to flow between the reservoir and the end of the sensing chamber outlet and over the barrier.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Goods & Services

Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; laboratory and scientific portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting-on-dielectric (EWOD) devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; cartridges being laboratory and scientific apparatus for holding biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents other than for medical purposes; reaction units containing chemical or biological reagents being laboratory equipment other than for medical use; nanopore sensing devices; parts and fittings for all the aforesaid goods; none of the aforesaid relating to entertainment, video games and interactive applications for entertainment purposes. Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; medical and veterinary apparatus using electrowetting technology; automated apparatus and instruments for the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, for medical purposes; nanopore sensing devices for medical purposes; apparatus and instruments for medical diagnosis; portable apparatus and instruments for medical diagnosis; parts and fittings for all the aforesaid goods.

Goods & Services

Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting-on-dielectric (EWOD) devices; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; cartridges being laboratory and scientific apparatus for holding biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents; reaction units containing chemical or biological reagents being laboratory equipment other than for medical use; Nanopore sensing devices; parts and fittings for all the aforesaid goods. Surgical, medical and veterinary apparatus and instruments; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; electrowetting devices for medical and veterinary purposes; apparatus and instruments for automating the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, for medical purposes; Nanopore sensing devices; parts and fittings for all the aforesaid goods; apparatus and instruments for medical diagnosis; portable apparatus and instruments for medical diagnosis.

Abstract

A kit comprising first and second component parts adapted for connection to each other, wherein: the first component part comprises: a first array of electrical connectors; two substantially parallel lateral walls, one provided on either side of the first array of electrical connectors at a predetermined position with respect to the first array of electrical connectors; two rails provided between the first array of electrical connectors and the lateral walls, one rail being on either side of the first array of electrical connectors, wherein each rails extends at least along a length of the first array of electrical connectors, and has a front tip positioned at a predetermined position with respect to the first array of electrical connectors; a front contact point; an overhang for receiving the second component; the second component part comprises: a second array of electrical connectors, for connection to the first array of electrical connectors; a front end being configured to fit to a width between the parallel lateral walls, lateral sides, each comprising a rail relief to allow the sides to fit around the two rails, and wherein a shoulder is formed by the front end of each rail relief; and wherein the first and component are configured such that they may be connected by sliding the front end of the second component along the two rails of the first component and under the overhang, so that the shoulders of the rail reliefs of the second component pass the front tips of the rails of the first component, and the front end of the second component bears against the front contact point of the first component, urging the shoulders of the rail reliefs against the front tips of the rails, thereby locating the second array of electrical connectors in the correct position for connecting to the first array of electrical connectors.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• H01R 13/629 - Additional means for facilitating engagement or disengagement of coupling parts, e.g. aligning or guiding means, levers, gas pressure

Abstract

The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.

IPC Classes  ?

• G01N 33/483 - Physical analysis of biological material
• C07K 16/12 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from bacteria
• C12Q 1/6869 - Methods for sequencing
• C07K 14/245 - Escherichia (G)
• G01N 33/487 - Physical analysis of biological material of liquid biological material

Abstract

An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.

IPC Classes  ?

• G01N 27/00 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
• G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
• G01N 15/00 - Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
• G01N 27/447 - Systems using electrophoresis
• G01N 33/487 - Physical analysis of biological material of liquid biological material
• C12Q 1/6869 - Methods for sequencing
• G01N 15/14 - Electro-optical investigation

Goods & Services

Analysis and evaluation of marketing, business and commercial data; provision of reports relating to the aforesaid; business services; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services connected with the sale of data analysis products and services and data streaming products and services; retail services connected with the sale of electronic sensing and measuring equipment, apparatus and instruments; retail services connected with the sale of sequencing equipment, apparatus and instruments; retail services connected with the sale of data processing equipment, apparatus and instruments; retail services connected with the sale of scientific equipment, apparatus and instruments; retail services connected with the sale of surgical, medical and veterinary equipment, apparatus and instruments; retail services connected with the sale of reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail services connected with the sale of chemicals and enzymes; retail services connected with the sale of chemicals and enzymes for scientific, medical or veterinary purposes; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail services connected with the sale of goods for use in a laboratory, namely, electronic apparatus for automating the extracting and preparation of test samples; information, advisory and consultancy services relating to the aforesaid.

Goods & Services

Electronic sensing apparatus; scientific, nautical, surveying, optical, weighing, measuring, signalling and supervision apparatus and instruments; apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions; portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, medical diagnosis, measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; monitoring apparatus and instruments; scientific apparatus and instruments; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; batteries; rechargeable batteries; chargers for batteries; power packs; Nanopore sensing devices; parts and fittings for all the aforesaid goods. Medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy devices; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; reaction units containing chemical reagents, for medical purposes; Nanopore sensing devices; parts and fittings for all the aforesaid goods; apparatus and instruments for medical diagnosis. Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; services relating to the synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of data, including scientific and medical data; overseeing, monitoring and managing scientific and medical experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical data; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, mobile phones and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid.

Abstract

The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.

IPC Classes  ?

• C07K 14/245 - Escherichia (G)

Abstract

The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 9/22 - Ribonucleases
• C12N 9/52 - Proteinases derived from bacteria
• C07K 14/31 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
• C12N 9/90 - Isomerases (5.)
• C12N 9/96 - Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research. Computer software; software; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors; micro-array chips; electronic sensing apparatus; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments; apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis and measurement of ions; portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis and measurement of ions; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; apparatus for the analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids; apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis; diagnostic and testing apparatus, not for medical purposes; electric and electronic monitoring apparatus and instruments; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; instruments and apparatus for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips; biosensor chips; cartridges for test samples for use in scientific tests and experiments; batteries; rechargeable batteries; chargers for batteries; power packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices; testing apparatus incorporating chemical or biological reagents; reaction units containing chemical or biological reagents, being laboratory apparatus for non-medical use; nanopore sensing devices; parts and fittings for all the aforesaid goods. Scientific and technological services and research and design relating thereto; industrial analysis and research services; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; services relating to the synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; scientific research for medical or veterinary purposes; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services; DNA and polynucleic acid testing services; genome sequencing services; analysis and evaluation of scientific data, including scientific and medical research data; computerised analysis of data; overseeing, monitoring and managing scientific and medical research experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical research data; design of scientific experiments; analysis of data generated by scientific and medical research experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services for scientific research purposes; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services for scientific research purposes; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical research experiments, the results of scientific and medical research experiments and tests, the analysis and evaluation of scientific and medical research data, and data generated by scientific and medical research experiments and tests; scientific and medical research and development services; technical data analysis and evaluation services; electronic data storage services; application service provider (ASP) services for providing access to computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; information, advisory and consultancy services relating to the aforesaid.

Goods & Services

Preparation of business information reports; business information services; retail store services featuring software; retail store services featuring computer operating software; retail store services featuring data analysis and data streaming software and hardware; retail store services featuring electronic sensors for the analysis of scientific test samples; retail store services featuring sequencing equipment, apparatus and instruments, namely, electronic sensing apparatus for analyzing chemical, biochemical, biological, bacteriological and microbiological test samples; retail store services featuring data processing equipment; retail store services featuring surgical, medical and veterinary equipment, apparatus and instruments; retail store services featuring reagents, buffer solutions, solvents, surfactants, oils and other media for scientific, medical or veterinary purposes; retail store services featuring industrial chemicals and enzymes; retail store services featuring chemicals and enzymes for scientific, medical or veterinary purposes; retail store services featuring deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; information, advisory and consultancy services relating to the aforesaid

Goods & Services

Downloadable software for running an e-commerce platform, namely, software that allows users to perform electronic business transactions over the Internet; downloadable cloud computing software for running an e-commerce platform, namely, software that allows users to perform electronic business transactions over the Internet; downloadable software for use in analysing, transmitting and receiving data via a global communications network; downloadable software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; downloadable computer operating software; downloadable operating system software; downloadable operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; laboratory equipment, namely, micro-array chips; electronic sensing apparatus for analysing chemical, biochemical, biological, bacteriological and microbiological test samples for scientific testing services; laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples and the measurement of ions; laboratory and scientific portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples and the measurement of ions; scientific apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; scientific apparatus for the analysis of polynucleic acid sequences; scientific apparatus and instruments for the analysis of nucleic and polynucleic acids; scientific apparatus and instruments for the analysis of proteins; scientific apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis, namely, empty cartridges for test samples, namely, cartridges for use with concentrates and solutions in solid or liquid form samples for scientific or research testing use; downloadable computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; electrowetting- on-dielectric (EWOD) devices for the display of scientific data; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments for the analysis and recordal of scientific data; scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; electronic display interfaces for the display of information obtained from scientific equipment; silicon chips; DNA chips; biosensor chips for scientific research and scientific testing use; cartridges namely, cartridges for test samples and cartridges for use with concentrates and solutions in solid or liquid form for use in biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis; batteries; rechargeable batteries; chargers for batteries; power packs namely, battery packs; computer docking stations; testing apparatus incorporating chemical or biological reagents for medical purposes, namely, apparatus for testing gas liquids and solids; reaction units containing chemical or biological reagents being laboratory equipment other than for medical use; nanopore sensing devices Medical and veterinary testing and diagnostic apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; electrowetting-on-dielectric (EWOD) devices for medical and veterinary purposes; automated medical apparatus and instruments for the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary testing and diagnostic apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy instruments; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; cartridges for test samples and cartridges for use with concentrates and solutions in solid or liquid form for medical use, detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, namely, reaction systems for medical testing purposes; nanopore sensing devices for medical purposes

Goods & Services

Downloadable software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; laboratory equipment, namely, micro-array chips; electronic sensing apparatus for analysing chemical, biochemical, biological, bacteriological and microbiological test samples for scientific testing services; laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples and the measurement of ions; laboratory and scientific portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples and the measurement of ions; scientific apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing; scientific apparatus for the analysis of polynucleic acid sequences; scientific apparatus and instruments for the analysis of nucleic and polynucleic acids; scientific apparatus and instruments for the analysis of proteins; scientific apparatus and instruments for analysis of genetic information, genomic information and proteomic information; apparatus and instruments for use in laboratory analysis, namely, empty cartridges for test samples in the nature of cartridges for use with concentrates and solutions in solid or liquid form samples for scientific or research testing use; downloadable computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; electrowetting-on-dielectric (EWOD) devices for the display of scientific data; scientific apparatus and instruments for automating the extraction and preparation of test samples; scientific apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments for the analysis and recordal of scientific data; scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; electronic display interfaces for the display of information obtained from scientific equipment; silicon chips; DNA chips; biosensor chips for scientific research and scientific testing use; cartridges namely, cartridges for test samples and cartridges for use with concentrates and solutions in solid or liquid form for use in biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis; batteries; rechargeable batteries; chargers for batteries; power packs namely, battery packs; computer docking stations; testing apparatus incorporating chemical or biological reagents for medical purposes, namely, apparatus for testing gas, liquids and solids; reaction units containing chemical or biological reagents being laboratory equipment other than for medical use; scientific apparatus, namely, nanopore sensing devices; none of the aforesaid relating to entertainment, video games and interactive applications for entertainment purposes; none of the foregoing for clearing and settling exchange traded derivatives or smart card issuance and management, nor relating to quality monitoring and assessment of services, customer services, business processes and employee skills and performance Medical and veterinary testing and diagnostic apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; electrowetting-on-dielectric (EWOD) devices for medical and veterinary purposes; automated medical apparatus and instruments for the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary testing and diagnostic apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy instruments; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; cartridges for test samples and cartridges for use with concentrates and solutions in solid or liquid form for medical use, detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, namely, reaction systems for medical testing purposes, not for research purposes; medical apparatus, namely, nanopore sensing devices for medical purposes

Abstract

Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.

IPC Classes  ?

• C07K 14/245 - Escherichia (G)
• C12Q 1/6869 - Methods for sequencing

Goods & Services

(1) Computer e-commerce software to allow users to perform electronic business transactions via a global computer network; Software for running an e-commerce platform in the fields of scientific research and scientific analysis; sensors used in electronic scientific equipment for detecting, manipulating and analysing biological, bacteriological, chemical and biochemical matter, DNA, RNA, proteins, polynucleic acids and small molecules; micro-array chips; electronic sensing apparatus, namely sensors used in electronic scientific equipment for detecting, manipulating and analysing biological, bacteriological, chemical and biochemical matter, DNA, RNA, proteins, polynucleic acids and small molecules; apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical and electrochemical analysis, medical diagnosis, measurement of ions, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICs (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis for scientific and research purposes; portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical and electrochemical analysis, medical diagnosis, measurement of ions, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICs (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis for scientific and research purposes; scientific apparatus and instruments for sequencing DNA, RNA, protein and polynucleic acids; scientific apparatus and laboratory devices for the scientific analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids, namely, microarrays, and silicon chip based arrays for scientific and research purposes; apparatus and instruments for the analysis of proteins, namely, microarrays, and silicon chip based arrays for scientific and research purposes; apparatus and instruments for the analysis of genetic information, genomic information and proteomic information, namely, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICS (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis; apparatus and instruments for use in laboratory analysis, namely, cartridges for use with concentrates and solutions in solid and liquid form samples for scientific and research testing; diagnostic and testing apparatus for scientific and research purposes, namely, laboratory test tubes, centrifuges for laboratory use, constant temperature incubators for laboratory use, vacuum distillation columns for laboratory use; scientific apparatus and instruments for sequencing and synthesising chemical compounds, small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides for scientific and research purposes; scientific apparatus and instruments for automating the extraction and preparation of biological, bacteriological, chemical and biochemical material and DNA, RNA, proteins, polynucleic acids and small molecule of test samples; portable scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing and synthesising chemical compounds small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides for use in the fields of genomics, medical sciences, environmental research, agriculture, general health and well-being, national security and military defence; silicon chips; DNA chips; analyte detector chips; biosensor chips; empty cartridges for test samples, namely, cartridges for use with concentrates and solutions in solid and liquid form samples for scientific and research testing; batteries, rechargeable batteries, chargers for batteries, and power packs for use with electronic scientific equipment; laboratory testing apparatus incorporating chemical and biological reagents for testing gas, liquids and solids; reaction units containing chemical and biological reagents for laboratory use; Nanopore sensors for scientific testing and research purposes (2) Medical and veterinary apparatus and instruments, namely, automated electronic devices for sequencing DNA, RNA, and other small molecules for the purposes of analysing test samples comprising biological, chemical, biochemical, DNA, RNA, proteins and other small molecules for medical and veterinary purposes; automated electronic apparatus and instruments for the purpose of extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus, namely, automated electronic devices for sequencing DNA, RNA, and other small molecules for the purposes of analyzing test samples comprising biological, chemical, biochemical, DNA, RNA, protein and other small molecules for medical and veterinary purposes; liquid biopsy devices, namely biopsy needles; blood testing apparatus for chemical, biochemical and biological analysis of blood samples; diagnostic and analytical apparatus for medical and veterinary purposes, namely, automated electronic devices for sequencing DNA, RNA and other nucleic acids for the purposes of detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases and conditions; portable and analytical diagnostic apparatus for medical and veterinary purposes, namely, automated electronic devices for sequencing DNA, RNA and other nucleic acids for the purposes of detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases and conditions; testing apparatus, namely, automated electronic devices for sequencing DNA, RNA and other small molecules for medical and veterinary purposes; portable testing apparatus, namely, automated electronic devices for sequencing DNA, RNA and other small molecules for medical and veterinary purposes; cartridges for test samples, for medical purposes; reaction units containing chemical reagents for medical testing purposes; Nanopore sensors for medical and veterinary purposes

Goods & Services

Chemical diagnostic reagents for scientific use; reagents for use in automated test systems; reagents for analytical tests; reagents for industrial use; reagents for use in science; reagents for laboratory use; reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; reagents for nucleic acid amplification; reagents for use in forensic analysis; reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids; reagents for testing aqueous solutions; deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid; ribonucleic acid; peptide nucleic acids; locked nucleic acids; nucleic acids; oligonucleotides; oligosaccharides; proteins; peptides; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis; endonucleases, polymerases, ligases, glycosylases; nucleotides, pyrimidines, purines; amino acids; saccharides; reagents for biological processing; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; chemicals for use in tests to detect diseases in plant life; reagents for formation of amphipathic membranes; buffer solutions; aqueous solvents; non-aqueous solvents; isopropanol; ethanol; phenol; non-aqueous media; surfactants; silicone oils; silicone fluids; enzymes; lytic enzymes including lysozyme, lyticase and cellulose; proteases; nucleases, including RNase, DNase and restriction enzymes; chaotropes, including guanidine-HCl and guanidinium thiocyanate; detergents for industrial and scientific use; proteins for use in analytical tests; all the aforesaid other than for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for use in medical and scientific research Reagents for use in automated test systems for medical or veterinary use; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, and surfactants, all for medical or veterinary use; reagents for medical and veterinary diagnosis and medical and veterinary research; reagents for laboratory tests for medical or veterinary purposes; nucleic acid amplification reagents for veterinary or medical use; reagents for immunoassay analysers for medical or veterinary use; reagents for testing aqueous solutions for medical or veterinary use; reagents for testing for susceptibility to allergies for medical or veterinary use; reagents for use in biological processing for medical or veterinary use; reagents for use in nucleic acid characterisation and nucleic acid sequencing for medical or veterinary use; reagents for protein identification and analysis for medical or veterinary use; reagents for formation of amphipathic membranes for medical or veterinary use; buffer solutions for medical or veterinary use; aqueous solvents for medical or veterinary use; non-aqueous solvents for medical or veterinary use; non-aqueous media for medical or veterinary use; surfactants for medical or veterinary use; silicone oils for medical or veterinary use; enzymes for medical or veterinary use; proteins for use in tests for medical or veterinary purposes; magnetic and non-magnetic microspheres made from synthetic materials including rubber, plastics or silicone, namely, beads for medical or veterinary purposes Computer software for performing and controlling the analysis of data obtained from scientific tests and experiments; software for running an e-commerce platform; software for use in analysing, transmitting and receiving data via a global communications network; software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; operating system software; operating software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; sensors used in electronic scientific equipment for detecting, manipulating and analysing biological, bacteriological, chemical and biochemical matter, DNA, RNA, proteins, polynucleic acids and small molecules; scientific equipment, namely, micro-array chips for the analysis and testing of biological, bacteriological, chemical and biochemical test samples, DNA, RNA, proteins, polynucleic acids and small molecules; electronic sensing apparatus for analysing chemical, biochemical, biological, bacteriological and microbiological test samples for scientific testing services; scientific apparatus and instruments for sequencing DNA, RNA, protein and polynucleic acids; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, supervision, life-saving and teaching apparatus and instruments, namely, apparatus for testing gas, liquids and solids, none of the aforesaid being eyewear; apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical or electrochemical analysis, measurement of ions, namely, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICs (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis for scientific or research purposes; apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing namely, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICS (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis; apparatus for the analysis of polynucleic acid sequences, namely, microarrays, and silicon chip based arrays for scientific or research purposes; apparatus and instruments for the analysis of nucleic and polynucleic acids, namely, microarrays, and silicon chip based arrays for scientific or research purposes; apparatus and instruments for the analysis of proteins namely, microarrays, and silicon chip based arrays for scientific or research purposes; apparatus and instruments for analysis of genetic information, genomic information and proteomic information, namely, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICS (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis; apparatus and instruments for use in laboratory analysis, namely, empty cartridges for test samples, namely, cartridges for use with concentrates and solutions in solid or liquid form samples for scientific or research testing use; diagnostic and testing apparatus for testing air quality, food, soil or water, not for medical purposes; electric and electronic monitoring apparatus and instruments, namely, electric monitors and monitor modules for monitoring electric current and electrical signals; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific research and scientific testing purposes; electrowetting-on-dielectric (EWOD) devices for the display of scientific data; scientific apparatus and instruments for automating the extraction and preparation of test samples; apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; data processing apparatus, devices and instruments; scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific research and scientific testing purposes; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; computer peripheral devices; electronic display interfaces for the display for the display of information obtained from scientific equipment; silicon chips; DNA chips; analyte detector chips for scientific research and scientific testing use; biosensor chips for scientific research and scientific testing use; cartridges for test samples and cartridges for use with concentrates and solutions in solid or liquid form for use in biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis; batteries; rechargeable batteries; chargers for batteries; power packs, namely, battery packs; computer docking stations; apparatus for connecting and charging portable digital electronic devices namely, battery packs; testing apparatus incorporating chemical or biological reagents for medical purposes, namely, apparatus for testing gas liquids and solids; reaction units containing chemical or biological reagents being laboratory equipment other than for medical use; Nanopore sensing devices; parts and fittings for all the aforesaid goods Surgical, medical and veterinary diagnostic apparatus and instruments for the testing and analysis of DNA, RNA, and other genetic material; medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; medical and veterinary apparatus using electrowetting technology; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; liquid biopsy instruments; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; diagnostic apparatus and instruments in the nature of reaction units containing chemical reagents, namely, reaction systems for medical testing purposes; medical apparatus, namely, nanopore sensing devices for medical purposes; parts and fittings for all the aforesaid goods; none of the foregoing for or related to medical devices for use with endoscopes, tissue snares, or tissue retrieval devices Scientific and technological services and research and design relating thereto, namely, consultation, research and design services relating to automated systems and instruments for direct electrical detection and analysis of molecules for application in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defence; industrial analysis and research services in the fields of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defence; scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services, namely, biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis for scientific or research purposes; scientific and technological services, namely, synthesizing chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific testing purposes; scientific research for medical or veterinary purposes; scientific and technological services, namely, genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services for scientific research purposes; scientific and technological services, namely, synthesising chemical compounds and small molecules for scientific and technological applications; DNA and RNA and polynucleic acid sequencing services for scientific and research purposes; protein sequencing services for scientific research and scientific testing purposes; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services in the fields of chemical, biochemical, biological, bacteriological, genomic and molecular analysis, for scientific testing and scientific, medical and veterinary purposes; DNA and polynucleic acid testing services; genome sequencing services for scientific and research purposes; analysis and evaluation of data in the field of biology, chemistry, biochemistry, bacteriology, DNA and RNA for scientific testing and scientific research purposes; computerised analysis of scientific and medical research data for scientific research purposes; overseeing, monitoring and managing scientific and medical research and diagnostic experiments for scientific research purposes; overseeing, monitoring and managing the analysis and evaluation of scientific and medical research data for scientific research purposes; design of scientific experiments; analysis of data generated by scientific and medical research experiments and tests for scientific and research purposes; scientific and technology services, namely, biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services for scientific research and scientific testing purposes and for medical research; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services for scientific testing and scientific research purposes; genome sequencing services and the analysis and evaluation of data resulting therefrom for scientific testing and scientific research purposes; provision of scientific information in the form of reports relating to scientific and medical research and diagnostic experiments, the results of scientific and medical research and diagnostic experiments, the analysis and evaluation of scientific and medical research data, and data generated by scientific and medical research and medical diagnostic experiments for scientific research purposes; scientific and medical research and development services; technical data analysis and evaluation services in the fields of scientific, medical technology and medical research; electronic data storage services; application service provider (ASP) services featuring computer software applications for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices for the analysis of scientific data; computerised analysis of medical research data, namely, the results of scientific tests and experiments for medical research purposes; overseeing, monitoring and managing medical research tests for medical research purposes; overseeing, monitoring and managing the analysis and evaluation of medical research data for medical research purposes; scientific and technological services, namely, biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services for medical research purposes; provision of scientific information in the form of reports relating to the results of medical research tests, the analysis and evaluation of medical research data, and data generated by medical research tests, none being for medical treatment or diagnostic purposes; information, advisory and consultancy services relating to the aforesaid

Goods & Services

(1) Analysis and evaluation of marketing, business and commercial data, provision of marketing, business and commercial reports; business information services, namely analysis and evaluation of business information in the fields of genomics, protein analysis, molecular analysis, chemistry, biochemistry, biology, biotechnology, microbiology and bacteriology, medical research, medical testing and diagnosis, national security and military defense; retail services connected with the sale of software; retail services connected with the sale of computer operating software; retail services connected with the sale of data analytic reports and marketing analysis and research services, and analysis and management of streamed genome sequencing data; Retail sale of electronic sensing and measuring equipment, apparatus and instruments for the analysis and sequencing of DNA, RNA and other polynucleic acids for scientific research and testing purposes; Retail sale of DNA sequencing equipment; retail services connected with the sale of computer hardware and computer software for data processing; retail services connected with the sale of scientific apparatus and instruments for sequencing DNA, RNA, proteins and polynucleic acid, namely, electronic nanopore sensors, flow cells containing biological reagents for creating chemical reactions for the purposes of analyzing and testing biological, bacteriological, chemical, biochemical, DNA, RNA, protein and molecular test samples; retail services connected with the sale of surgical instruments, veterinary and medical supplies; retail services connected with the sale of reagents for formation of amphipathic membranes, chemical buffer solutions, non-aqueous solvents, silicone oils and surfactants, all for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis; retail services connected with the sale of chemicals and enzymes for use in scientific analytical, medical and veterinary tests in the fields of genomics, molecular biology, biotechnology, microbiology and bacteriology; retail services connected with the sale of deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid, ribonucleic acid, peptide nucleic acids, locked nucleic acids, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides; retail services connected with the sale of goods for use in a laboratory, namely, electronic apparatus for automating the extracting and preparation of test samples.

Goods & Services

(1) Chemical diagnostic reagents for use in scientific tests and experiments for scientific research purposes, chemical diagnostic reagents for use in automated test systems for medical and veterinary testing purposes and for use in scientific tests and experiments for scientific research purposes, reagents for analytical tests on samples for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, reagents for industrial use, namely, synthesising DNA, RNA and other nucleic acids, reagents for use in scientific tests, namely, the sequencing of DNA, RNA and other nucleic acids, reagents for laboratory tests for scientific research purposes, cell culture, chemical, diagnostic reagents for use in molecular biology, biotechnology, microbiology, and bacteriology, reagents for nucleic acid amplification, cell culture, chemical, diagnostic reagents for use in forensic analysis, reagents for blood grouping; reagents used for analytical moisture determinations, reagents for testing water and biological fluids, namely, blood, saliva, urine, interstitial fluid, serum, plasma, semen, tears, bile fluid, spinal fluid, and amniotic fluid, reagents for testing aqueous media for use in medical and veterinary tests for the purposes of identifying and diagnosing medical conditions, diseases and disorders, deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid for laboratory use and scientific testing and research use, ribonucleic acid for laboratory use and scientific testing and research use, peptide nucleic acids for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics, locked nucleic acids for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics, nucleic acids, oligonucleotides, oligosaccharides, proteins and peptides all for laboratory use and scientific testing and research use in the fields of molecular biology, biotechnology, microbiology, bacteriology and genomics, oligonucleotides for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, oligosaccharides for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, proteins for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, peptides for industrial purposes, scientific purposes and scientific research purposes, chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis, endonucleases, polymerases, ligases, glycosylases, all for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, nucleotides, pyrimidines, purines for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, amino acids for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, saccharides for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, reagents for use in nucleic acid characterisation and nucleic acid sequencing, reagents for protein identification and analysis, chemicals for use in tests to detect diseases in plant life, reagents for formation of amphipathic membranes, buffer solutions for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, aqueous solvents for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, non-aqueous solvents for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, isopropanol, ethanol, phenol, non-aqueous media for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, surfactants for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, silicone oils, silicone fluids, enzymes for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, lytic enzymes including lysozyme, lyticase and cellulose for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, proteases for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, nucleases, namely, RNase, DNase and restriction enzymes for use in analytical chemistry, biochemistry, biology, biotechnology, bacteriology, genomics and the sequencing of DNA, RNA and other nucleic acids, chaotropes agents, namely, guanidine HCl and guanidinium thiocyanate for use in scientific tests and experiments for scientific research purposes, chaotropic agents, namely, guanidine-HCI and guanidinium thiocyanate for use in diagnostic tests for medical and veterinary purposes, detergents for industrial use, detergents for use in the manufacture of pharmaceuticals, proteins for use in analytical tests for scientific testing and research purposes in the fields of genomics, life sciences, healthcare, national security and military defence, all the aforesaid other than for medical and veterinary purposes; unprocessed synthetic resin, plastic and silicone compounds in the form of microspheres used to incorporate various other substances. (2) Diagnostic chemical reagents for use in automated test systems for medical and veterinary use; reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, namely reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants; reagents for medical and veterinary diagnosis and medical and veterinary research; reagents for laboratory tests for medical and veterinary purposes; nucleic acid amplification reagents for veterinary, medical, medical research and forensic use; reagents for immunoassay analysers; diagnostic test reagents for testing aqueous solutions for medical and veterinary use; reagents for testing for susceptibility to allergies; diagnostic reagents for use in biological processing for medical and veterinary use; reagents for use in nucleic acid characterisation and nucleic acid sequencing; reagents for protein identification and analysis; reagents for formation of amphipathic membranes; surfactants for use in medical and veterinary diagnostic tests; proteins for use in diagnostic tests for medical and veterinary purposes; magnetic and non-magnetic albumin microspheres made from rubber, plastics or silicone, namely, beads that facilitate delivery of a wide range of pharmaceuticals. (3) Computer e-commerce software to allow users to perform electronic business transactions via a global computer network; Software for running an e-commerce platform in the fields of scientific research and scientific analysis; sensors used in electronic scientific equipment for detecting, manipulating and analysing biological, bacteriological, chemical and biochemical matter, DNA, RNA, proteins, polynucleic acids and small molecules; micro-array chips; electronic sensing apparatus, namely sensors used in electronic scientific equipment for detecting, manipulating and analysing biological, bacteriological, chemical and biochemical matter, DNA, RNA, proteins, polynucleic acids and small molecules; laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical and electrochemical analysis, medical diagnosis, measurement of ions, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICs (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis for scientific and research purposes; portable laboratory and scientific apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, optical and electrochemical analysis, medical diagnosis, measurement of ions, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICs (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis for scientific and research purposes; scientific apparatus and instruments for sequencing DNA, RNA, protein and polynucleic acids; scientific apparatus and laboratory devices for the scientific analysis of polynucleic acid sequences; apparatus and instruments for the analysis of nucleic and polynucleic acids, namely, microarrays, and silicon chip based arrays for scientific and research purposes; apparatus and instruments for the analysis of proteins, namely, microarrays, and silicon chip based arrays for scientific and research purposes; apparatus and instruments for analysis of genetic information, genomic information and proteomic information, namely, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICS (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis; apparatus and instruments for use in laboratory analysis, namely, cartridges for use with concentrates and solutions in solid and liquid form samples for scientific and research testing; diagnostic and testing apparatus for scientific and research purposes, namely, laboratory test tubes, centrifuges for laboratory use, constant temperature incubators for laboratory use, vacuum distillation columns for laboratory use; scientific apparatus and instruments for sequencing and synthesising chemical compounds, small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides for scientific and research purposes; scientific apparatus and instruments for automating the preparation of biological, bacteriological, chemical and biochemical material and DNA, RNA, proteins, polynucleic acids and small molecule of test samples; portable scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing and synthesising chemical compounds, small molecules, genomes, DNA, RNA and other nucleic acids, oligosaccharides, proteins and peptides for use in the fields of genomics, medical sciences, environmental research, agriculture, general health and well-being, national security and military defence; silicon chips; DNA chips; analyte detector chips; biosensor chips; solid phase extraction cartridges for laboratory use; batteries, rechargeable batteries, chargers for batteries, and power packs for use with electronic scientific equipment; computer docking stations; laboratory testing apparatus incorporating chemical and biological reagents for testing gas, liquids and solids; reaction units containing chemical and biological reagents for laboratory use; Nanopore sensors for scientific testing and research purposes. (4) Surgical, medical and veterinary apparatus and instruments, namely, automated electronic devices for sequencing DNA, RNA, and other small molecules for the purposes of analysing test samples comprising biological, chemical, biochemical, DNA, RNA, proteins and other small molecules for medical and veterinary purposes; automated electronic apparatus and instruments for the purpose of preparation of test samples for medical and veterinary purposes; portable medical and veterinary apparatus, namely, automated electronic devices for sequencing DNA, RNA, and other small molecules for the purposes of analyzing test samples comprising biological, chemical, biochemical, DNA, RNA, proteins and other small molecules for medical and veterinary purposes; liquid biopsy devices, namely biopsy needles; blood testing apparatus for chemical, biochemical and biological analysis of blood samples; diagnostic and analytical apparatus for medical and veterinary purposes, namely, automated electronic devices for sequencing DNA, RNA and other nucleic acids for the purposes of detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases and conditions; portable and analytical diagnostic apparatus for medical and veterinary purposes, namely, automated electronic devices for sequencing DNA, RNA and other nucleic acids for the purposes of detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases and conditions; testing apparatus, namely, automated electronic devices for sequencing DNA, RNA and other small molecules for medical and veterinary purposes; portable testing apparatus, namely, automated electronic devices for sequencing DNA, RNA and other small molecules for medical and veterinary purposes; reaction units containing chemical reagents, for testing medical purposes; Nanopore sensors for medical and veterinary purposes. (1) Scientific and technological services and research and design relating thereto, namely, consultation, research and design services relating to automated systems and instruments for direct electrical detection and analysis of molecules for use in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, national security and military defence; industrial analysis and research services in the fields of genomics, protein analysis, molecular analysis, life sciences, healthcare, national security and military defence; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services; services relating to the synthesis of chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides; genome, DNA, RNA and nucleic acid, oligosaccharide, protein and peptide synthesising services; chemical compound and small molecule synthesising services; DNA and RNA and polynucleic acid sequencing services; protein sequencing services; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services in the fields of genomics, protein analysis, molecular analysis, chemistry, biochemistry, biology, biotechnology, microbiology and bacteriology; DNA and polynucleic acid testing services; genome sequencing services; Analysis and evaluation in the fields of genomics, protein analysis, molecular analysis, chemistry, biochemistry, biology, biotechnology, microbiology and bacteriology; overseeing, monitoring and managing scientific and medical experiments and tests; overseeing, monitoring and managing the analysis and evaluation of scientific and medical data; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services; genome sequencing services and the analysis and evaluation of data resulting therefrom; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests; medical research; technical and evaluation services in the fields of chemistry, biology, biotechnology, genomics, pharmaceutical development, medical research and nanotechnology; electronic storage of medical records; application service provider (ASP) services featuring application programming interface software for analysis of scientific and medical records obtained from sequencing DNA, RNA and other polynucleic acids and installed and downloaded onto medical image processors, computers, tablet computers and handheld mobile digital electronic devices.

Abstract

The invention relates to a method of determining the presence or absence of a target analyte in a sample. The method comprises immobilising any target analyte present in the sample on a surface; contacting the surface with: (i) a first detection agent that binds specifically to the target analyte; and (ii) a reporter polynucleotide, wherein the reporter polynucleotide is bound to, or binds to, the first detection agent; and contacting a transmembrane pore with any reporter polynucleotide that has been immobilised on the surface, wherein the reporter polynucleotide is immobilised on the surface by binding of the first agent to the target analyte, and using the transmembrane pore to detect the reporter polynucleotide, thereby determining the presence or absence of the target analyte in the sample.

IPC Classes  ?

• G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/52 - Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper

Abstract

A series of measurements taken from a polymer during translocation through a nanopore is analysed using a machine learning technique using a recurrent neural network (RNN). The RNN may derive posterior probability matrices each representing, in respect of different respective historical sequences of polymer units corresponding to measurements prior to the respective measurement, posterior probabilities of plural different changes to the respective historical sequence of polymer units giving rise to a new sequence of polymer units. Alternatively, the RNN may output decisions on the identity of successive polymer units of the series of polymer units, wherein the decisions are fed back into the recurrent neural network. The analysis may comprise performing convolutions of groups of consecutive measurements using a trained feature detector such as a convolutional neural network to derive a series of feature vectors, on which the RNN operates.

IPC Classes  ?

• G06N 3/04 - Architecture, e.g. interconnection topology
• C12Q 1/6869 - Methods for sequencing
• G01N 33/487 - Physical analysis of biological material of liquid biological material