09 - Appareils et instruments scientifiques et électriques
Produits et services
Downloadable software for running an e-commerce platform,
namely, software that allows users to perform electronic
business transaction over the Internet; cloud computing
software for running an e-commerce platform, namely,
software that allows users to perform electronic business
transaction over the Internet; downloadable software for use
in analysing, transmitting and receiving data via a global
communications network; cloud computing software for use in
analysing, transmitting and receiving data via a global
communications network; downloadable software for
developing, running and communicating with software on data
processing apparatus, scientific apparatus, medical and
veterinary apparatus, computers, mobile phones, tablet
computers and handheld mobile digital electronic devices;
computer operating software; computer operating system
software; computer operating system software for data
processing apparatus, scientific apparatus, medical and
veterinary apparatus, computers, mobile phones, tablet
computers and handheld mobile digital electronic devices;
laboratory equipment, namely, micro-array chips; electronic
sensing apparatus for analysing chemical, biochemical,
biological, bacteriological and microbiological test samples
for scientific testing purposes; scientific testing
apparatus and instruments, namely, optical or
electrochemical readers for biological, bacteriological,
chemical, biochemical, DNA, RNA, protein, polynucleic acid
and molecular test samples and the measurement of ions;
portable apparatus and instrument, namely, optical or
electrochemical readers for biological, bacteriological,
chemical, biochemical, DNA, RNA, protein, polynucleic acid
and molecular test samples, and measurement of ions;
scientific instruments, namely, sequencing devices for use
in DNA, RNA, protein and polynucleic acid sequencing;
computer software and optical or electrochemical readers for
the analysis of polynucleic acid sequences; scientific
apparatus and instruments for the analysis of nucleic and
polynucleic acids; scientific apparatus and instruments for
the analysis of proteins; apparatus and instruments for
analysis, not for medical use, of genetic information,
genomic information and proteomic information, namely,
automated instruments for detecting the presence, properties
and identity of analytes, comprising cartridges,
microarrays, silicon chip based arrays, ASICS (application
specific integrated circuits), and networked computer
hardware and software for performing and controlling the
analysis, and for processing, displaying and storing the
information obtained from the analysis; computer programs,
computer hardware and data processing apparatus, all for the
analysis and recordal of scientific data;
electrowetting-on-dielectric (EWOD) devices; scientific
apparatus and instruments for automating the extraction and
preparation of chemical, biochemical, biological,
bacteriological and microbiological test samples; data
processing apparatus, devices and instruments; scientific
apparatus, devices and instruments for identifying,
measuring, analysing, sequencing small molecules, genomes,
DNA, RNA or other nucleic acids, oligosaccharides, proteins
and peptides for scientific research and scientific testing
purposes; portable computer hardware and data processing
apparatus, all for the analysis and recordal of scientific
data; electronic display interfaces for the display of
information obtained from scientific equipment; silicon
chips; DNA chips; biosensor chips for scientific research
and scientific testing use; cartridges namely, cartridges
for test samples and cartridges for use with concentrates
and solutions in solid or liquid form for use in biological,
bacteriological, chemical, biochemical, DNA, RNA, protein,
polynucleic acid and molecular analysis, not for medical
use.
2.
METHOD OF DETERMINING A TARGET POLYMER IN A SAMPLE BY USING A GUIDE POLYMER
The invention relates generally to a method of detecting and/or analysing target polymers, especially target polynucleotides, using a biological pore. The invention also relates to a novel system for carrying out the method. The method has many uses. In particular, the method may be used for diagnosis, detection of polymorphisms and V(D)J repertoire analysis.
C12Q 1/44 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir une hydrolase une estérase
C12Q 1/6825 - Détecteurs faisant intervenir la détection d’acides nucléiques
Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements. Two or more electrodes are configurable to lyse cells within a droplet positioned over said electrodes.
Methods and apparatus for measuring current are provided. In one arrangement, a first charge amplifier integrates a current to be measured. A processing circuit filters an output from the first charge amplifier using a first low pass filter module and a second low pass filter module. A second charge amplifier integrates a current derived from the filtered output from the first charge amplifier. The apparatus is configured to reset the first charge amplifier at the start of each of a plurality of sensing frames. The processing circuit obtains at least a first sample of the output from the first charge amplifier in each sensing frame. The sampling of the first sample alternates from one sensing frame to the next sensing frame between sampling via the first low pass filter module and sampling via the second low pass filter module.
G01N 27/12 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'impédance en recherchant la résistance d'un corps solide dépendant de la réaction avec un fluide
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
5.
METHOD AND PRODUCTS FOR CHARACTERIZING A POLYNUCLEOTIDE USING A NANOPORE
Provided herein are methods of characterising a polynucleotide during the translocation of the polynucleotide through a nanopore. Also provided herein are methods of operating a nanopore array. Further provided herein are kits and apparatuses for carrying out such methods.
A modular instrument (1) for preparing and sensing an analyte in a prepared sample. The modular instrument includes a main body (12), an EWOD sample preparation device (2), and a nanopore sensor device (3) for sensing the analyte. The main body is electrically connectable to and separable from both the EWOD sample preparation device and the nanopore sensor device. The main body, the EWOD sample preparation device and nanopore sensor are physically connectable to and separable from each other.
The invention relates to modified helicases which are capable of controlling the movement of polynucleotides with increased speed. The modified helicases are particularly useful for sequencing polynucleotides.
C07K 14/31 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de bactéries provenant de Micrococcaceae (F) provenant de Staphylococcus (G)
A series of measurements taken from a polymer during translocation through a nanopore is analysed using a machine learning technique using a recurrent neural network (RNN). The RNN may derive posterior probability matrices each representing, in respect of different respective historical sequences of polymer units corresponding to measurements prior to the respective measurement, posterior probabilities of plural different changes to the respective historical sequence of polymer units giving rise to a new sequence of polymer units. Alternatively, the RNN may output decisions on the identity of successive polymer units of the series of polymer units, wherein the decisions are fed back into the recurrent neural network. The analysis may comprise performing convolutions of groups of consecutive measurements using a trained feature detector such as a convolutional neural network to derive a series of feature vectors, on which the RNN operates.
An apparatus for supporting an array of layers of amphiphilic molecules, the apparatus comprising: a body, formed in a surface of the body, an array of sensor wells capable of supporting a layer of amphiphilic molecules across the sensor wells, the sensor wells each containing an electrode for connection to an electrical circuit, and formed in the surface of the body between the sensor wells, flow control wells capable of smoothing the flow of a fluid across the surface.
The present invention relates to novel actinoporin monomers, actinoporin pores formed from the monomers and their uses in analyte detection and characterisation.
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
C07K 14/435 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant d'humains
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
Medical and veterinary testing and diagnostic apparatus for
biological, chemical, biochemical, DNA, RNA, protein and
molecular analysis; electrowetting devices for medical and
veterinary purposes; apparatus and instruments for
automating the extraction and preparation of test samples
for medical and veterinary purposes; portable medical and
veterinary testing and diagnostic apparatus for biological,
chemical, biochemical, DNA, RNA, protein and molecular
analysis; diagnostic apparatus for medical or veterinary
purposes for pregnancy testing, and for detecting
pathogenic, infectious, psychiatric, oncogenic, metabolic,
genetic, respiratory, genitourinary, digestive,
inflammatory, autoimmune, reproductive, hepatic, central
nervous system, degenerative and cardiovascular diseases or
conditions; portable diagnostic apparatus for medical or
veterinary purposes for pregnancy testing, and for detecting
pathogenic, infectious, psychiatric, oncogenic, metabolic,
genetic, respiratory, genitourinary, digestive,
inflammatory, autoimmune, reproductive, hepatic, central
nervous system, degenerative and cardiovascular diseases or
conditions; testing apparatus for medical or veterinary
purposes for detecting pathogenic, infectious, psychiatric,
oncogenic, metabolic, genetic, respiratory, genitourinary,
digestive, inflammatory, autoimmune, reproductive, hepatic,
central nervous system, degenerative and cardiovascular
diseases or conditions; portable testing apparatus for
medical or veterinary purposes for detecting pathogenic,
infectious, psychiatric, oncogenic, metabolic, genetic,
respiratory, genitourinary, digestive, inflammatory,
autoimmune, reproductive, hepatic, central nervous system,
degenerative and cardiovascular diseases or conditions;
cartridges for test samples, for medical purposes; reaction
units containing chemical reagents, namely, reaction systems
for medical testing purposes; Nanopore sensing devices for
medical and veterinary purposes.
The invention relates to a method of uniquely labelling RNA molecules in a population of cells and kits for use in such methods. The methods and kits of the invention allow the study of the transcriptome in individual cells or populations of cells.
DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES OEFFENTLICHEN RECHTS (Allemagne)
UNIVERSITAET HEIDELBERG (Allemagne)
OXFORD NANOPORE TECHNOLOGIES PLC (Royaume‑Uni)
Inventeur(s)
Sahm, Felix
Sill, Martin
Von Deimling, Andreas
Pfister, Stefan
Jones, David
Patel, Areeba
Dogan, Helin
Loose, Matt
Payne, Alexander
Abrégé
The present disclosure relates to a computer-implemented method for cancer diagnosis, comprising: a) selectively sequencing polymers of a biological sample according to at least one target gene site by translocating the polymers through nanopores of a nanopore sequencing system, including: (i) analyzing an initial nucleotide sequence of a first polymer of the biological sample while the first polymer is translocating through a nanopore of the nanopore sequencing system to determine whether the initial nucleotide sequence corresponds to the at least one target gene site; and (ii) continuing the sequencing of the first polymer to obtain measurement data of the first polymer only if the initial nucleotide sequence of the first polymer corresponds to the at least one target gene site: b) determining, based on the measurement data, a biological state of a nucleotide sequence of the first polymer corresponding to the at least one target gene site; and c) classifying a cancer using a classification algorithm based on the biological state of the nucleotide sequence of the first polymer, wherein the classification algorithm is trained based on the at least one target gene site and biological state data pertaining to cancer types.
G16H 50/20 - TIC spécialement adaptées au diagnostic médical, à la simulation médicale ou à l’extraction de données médicales; TIC spécialement adaptées à la détection, au suivi ou à la modélisation d’épidémies ou de pandémies pour le diagnostic assisté par ordinateur, p.ex. basé sur des systèmes experts médicaux
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 40/10 - Traitement du signal, p.ex. de spectrométrie de masse ou de réaction en chaîne par polymérase
The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.
The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.
Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown are provided. In one disclosed arrangement a plurality of apertures are formed. The membrane comprises a first surface area portion on one side of the membrane and a second surface area portion on the other side of the membrane. Each of a plurality of target regions comprises a recess or a fluidic passage opening out into the first or second surface area portion. The method comprises contacting all of the first surface area portion of the membrane with a first bath comprising ionic solution and all of the second surface area portion with a second bath comprising ionic solution. A voltage is applied across the membrane via first and second electrodes in respective contact with the first and second baths comprising ionic solutions to form an aperture at each of a plurality of the target regions in the membrane.
B01D 67/00 - Procédés spécialement adaptés à la fabrication de membranes semi-perméables destinées aux procédés ou aux appareils de séparation
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétés; Procédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
C25F 3/14 - Attaque de surface localisée, c. à d. gravure
C25F 7/00 - PROCÉDÉS POUR LE TRAITEMENT D'OBJETS PAR ENLÈVEMENT ÉLECTROLYTIQUE DE MATIÈRE; APPAREILLAGES À CET EFFET Éléments de construction des cellules, ou leur assemblage, pour l'enlèvement électrolytique de matières d'objets; Entretien ou conduite
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
A sensor device (902) for a nanopore sensor, the sensor device (902) comprising: an insulating substrate (903); one or more wells (908) for containing a fluid; wherein the one or more wells (908) are formed on a first side of the substrate (903); a sensor electrode (910) for detecting an ionic current in each of the one or more wells (908); wherein the sensor electrodes (910) are formed on the first side of the substrate (903) at the base of the one or more wells (908); one or more contacts (914) formed on a second side of the substrate (903); and one or more vias (912) extending through the substrate (903); wherein the one or more vias (912) connect the sensor electrodes (910) to the one or more contacts (914); wherein the electrodes (910) comprise a different structure from the one or more contacts (914) and/or the sensor electrodes (910) are made from a different material or materials from the one or more contacts (914).
A sensor device (902) for a nanopore sensor, the sensor device (902) comprising: an insulating substrate (903); one or more wells (908) for containing a fluid; wherein the one or more wells (908) are formed on a first side of the substrate (903); a sensor electrode (910) for detecting an ionic current in each of the one or more wells (908); wherein the sensor electrodes (910) are formed on the first side of the substrate (903) at the base of the one or more wells (910); one or more vias (912) extending through the substrate (903); wherein the one or more vias (912) are connected to sensor electrodes (910); wherein the one or more vias (912) each comprise a conducting cap (950) at one or both ends of the via (912).
A sensor device (902) for a nanopore sensing apparatus (901), the sensor device (902) comprising: an insulating substrate (903); one or more wells (908) for containing a fluid; wherein the one or more wells (908) are formed on a first side of the substrate (903); a sensor electrode (910) for detecting an ionic current in a respective well (908) of the one or more wells (908); wherein the one or more sensor electrodes (910) are formed on the first side of the substrate (903) at the base of the one or more wells (908); and wherein the base of each of the one or more wells (908) extends over a base area on the first side of the substrate (903); and one or more vias (912) extending through the substrate (903); wherein each of the one or more sensor electrodes (910) is connected to a respective via (912) of the one or more vias (912); and wherein each via (912) has a cross-sectional area at the first side of the substrate (903) that at least partially falls outside of the base area of each corresponding well (908).
A sensor device (902) for a nanopore sensor, the sensor device (902) comprising: an insulating substrate (903); one or more wells (908) for containing a fluid; wherein the one or more wells (908) are formed on a first side of the substrate (903); a sensor electrode (910) for detecting an ionic current in each of the one or more wells (908); wherein the sensor electrodes (910) are formed on the first side of the substrate (903) at the base of the one or more wells (908); one or more vias (912) extending through the substrate (903); wherein the one or more vias (912) are connected to the sensor electrodes (910); wherein the one or more vias (912) each comprise: a conductor (946) forming a conductive path through the via (912); and a material (948) arranged to retain the conductor (946) in the via; wherein the material (948) arranged to retain the conductor (946) in the via (912) is different from a material of the conductor (946).
The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
A time-ordered series of measurements of a polymer made during translocation of the polymer through a Nanopore are analysed. The measurements are dependent on the identity of k-mers in the Nanopore, a k-mer bring k polymer units of the polymer, where k is a positive integer. The method involves deriving, from the series of measurements, a feature vector of time-ordered features representing characteristics of the measurements; and determining similarity between the derived feature vector and at least one other feature vector.
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p.ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
G01N 27/02 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'impédance
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 30/10 - Alignement de séquence; Recherche d’homologie
A method of generating a polymer sequence using a nanopore sequencing device, the nanopore array device comprising a nanopore channel, the nanopore channel (35) formed in a membrane (32) separating two ionic solutions (33, 36), the nanopore channel connecting the ionic solutions, the method comprising: translocating a series of polymers through the nanopore channel; generating measurement signals during the translocation of each polymer through the nanopore channel; analysing a set of measurement signals from each polymer taken during the translocation of said polymer through the nanopore channel to determine a scaling factor for each polymer; applying the scaling factor to the measurement signals generated during the translocation of the polymer through the nanopore channel to determine normalised measurement signal values; and generating a polymer sequence for each polymer of the series from the corresponding normalised measurement signal values for each polymer.
An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.
An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.
An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.
Provided herein is a method of loading a motor protein onto a polynucleotide adapter. Also provided are polynucleotide adapters and kits comprising such adapters. The adapters find use in characterising analytes such as polynucleotides in methods in which the polynucleotide moves in respect of a nanopore.
The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
C07K 14/31 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de bactéries provenant de Micrococcaceae (F) provenant de Staphylococcus (G)
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p.ex. kinases (2.7)
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
Aspects of the disclosure relate to compositions and methods for characterizing nucleic acids using a nanopore. The disclosure is based, in part, on methods for increasing follow-on sequencing of nucleic acid strands. In some embodiments, the methods comprise increasing the concentration of a tethering agent. In some embodiments, the methods comprise use of adaptors having a rigid (or stiffened) leader section. Compositions and systems including, e.g., adaptors for attachment to double-stranded poly nucleotides and/or tethering agents, which can be used in the methods are also provided.
The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore: (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Reagents for use in automated test systems for non-medical purposes; Reagents for analytical tests for non-medical purposes; Reagents for industrial use, namely, for food testing, soil testing, water testing and air quality testing; Reagents for use in science; Reagents for laboratory use; Reagents for use in molecular biology, biotechnology, microbiology, Reagents for testing water and other liquids; Reagents for testing aqueous solutions; Reagents for biological processing prior to their use with or analysis by scientific apparatus; Reagents for use in nucleic acid characterisation and nucleic acid sequencing for non-medical purposes; Reagents for protein identification and analysis for non-medical purposes; Chemicals for use in tests to detect diseases in plant life; Reagents for formation of amphipathic membranes; Chemicals, namely, buffer solutions for scientific or research use; Nonaqueous media in the nature of solid or liquid non-aqueous solutions for scientific or research use; surfactants for use in analytical tests for scientific or research use; Silicone oils for analytical tests for scientific or research use; Silicone fluids; Enzymes for scientific and research purposes and use in analytical tests; Proteins for use in analytical tests for scientific or research use; Nucleic acid amplification reagents for veterinary, medical, medical research or forensic use; all the aforesaid other than for medical or veterinary purposes Reagents for use in automated test systems for veterinary or medical use; Reagents for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, all for medical or veterinary use; Reagents for medical and veterinary diagnosis; Reagents for laboratory tests for medical or veterinary purposes; Reagents for medical or veterinary use, namely, reagents, for immunoassay analysers; Reagents for medical or veterinary use, namely, reagents for testing aqueous solutions; Medical and veterinary diagnostic reagents for testing for susceptibility to allergies; Reagents for medical or veterinary use, namely, reagents for use in biological processing; Reagents for medical or veterinary use, namely, reagents for use in nucleic acid characterisation and nucleic acid sequencing; Reagents for medical or veterinary use, namely, reagents for protein identification and analysis; Reagents for medical or veterinary use, namely, reagents for formation of amphipathic membranes; buffer solutions for medical and veterinary diagnostic use; Aqueous solvents for use in tests for medical and veterinary purposes; non-aqueous solvents for use in tests for medical and veterinary purposes; Non-aqueous media in the nature of solid or liquid non-aqueous solutions for use in tests for medical and veterinary purposes; Silicone oil reagents for formation of amphipathic membranes for medical and veterinary use; Enzymes for use in tests for medical and veterinary purposes; proteins for use in tests, all for medical or veterinary purposes Apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, namely, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICs (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis for scientific or research purposes; portable apparatus and instruments for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis, namely, microarrays, and silicon chip based arrays for scientific or research purposes; Apparatus and instruments for use in DNA, RNA, protein and polynucleic acid sequencing, namely, portable electronic sequencing devices for scientific and research use; Apparatus and instruments for the analysis of nucleic and polynucleic acids, namely, microarrays, and silicon chip based arrays for scientific or research purposes; Apparatus and instruments for the analysis of proteins, namely, microarrays, and silicon chip based arrays for scientific or research purposes; Apparatus and instruments for analysis of genetic information, genomic information and proteomic information, namely, portable electronic devices for analysing the results of scientific tests and experiments; Diagnostic and testing apparatus, for testing air quality, food, soil or water, not for medical purposes; Downloadable or pre-recorded software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis for scientific or research purposes; Computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; Portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; Computer peripheral devices; Instruments and apparatus for the display of information obtained from scientific equipment, namely, electronic display interfaces; Silicon chips; DNA chips; Microarray chips; Analyte detector chips for scientific or research use; biosensor chips for scientific or research use; Laboratory equipment, namely, empty cartridges for use with concentrates and solutions in solid or liquid form samples for use in biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis; Batteries; Rechargeable batteries; Chargers for batteries; Computer docking stations; Apparatus for connecting and charging portable digital electronic devices; Testing apparatus incorporating chemical or biological reagents for testing air quality, food, soil or water; laboratory equipment, namely, reaction units containing chemical or biological reagents for scientific or research use; Nanopore sensing devices for scientific research and scientific testing use; parts and fittings for all the aforesaid goods Medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; portable medical and veterinary apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; blood testing apparatus; diagnostic apparatus for medical or veterinary purposes; portable diagnostic apparatus for medical or veterinary purposes; testing apparatus for medical or veterinary purposes; portable testing apparatus for medical or veterinary purposes; cartridges for test samples, for medical purposes; reaction units containing chemical reagents, for medical purposes; Nanopore sensing devices for medical purposes; parts and fittings for all the aforesaid goods Scientific and technological services and research and design relating thereto, namely, consultation, research and design services relating to automated systems and instruments for direct electrical detection and analysis of molecules for use in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defense; industrial analysis and research services in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defense; Scientific research for chemical, biochemical, biological, bacteriological, medical or veterinary purposes; biological, chemical, biochemical, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services, namely, biological, chemical, biochemical, bacteriological DNA, RNA, protein, polynucleic acid and molecular analysis for scientific or research purposes; DNA and RNA and polynucleic acid sequencing services for scientific and research purposes; Protein sequencing services for scientific and research purposes; Product design and development in the fields of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; Laboratory testing services for chemical, biochemical, biological, bacteriological, molecular analysis, medical or veterinary purposes; DNA and polynucleic acid testing services for chemical, biochemical, biological, bacteriological, molecular analysis, medical or veterinary purposes; Genome sequencing services for scientific testing, scientific research or clinical research purposes; Information and advice regarding all the aforesaid services
A sensor system comprising a sensor device, a hub component, and a tablet computer. The hub component is connectable to the sensor device, such that the hub component provides an interface for powering the sensor device and transferring data from the sensor device. The hub component further comprises a connection to a computer, such that the hub can deliver data collected from the sensor device to the computer. The height profile dimensions of the sensor device and the hub are similar to the height profile dimension of the tablet computer.
To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.
Medical and veterinary testing and diagnostic apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; electrowetting devices for medical and veterinary purposes; apparatus and instruments for automating the extraction and preparation of test samples for medical and veterinary purposes; portable medical and veterinary testing and diagnostic apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; cartridges for test samples, for medical purposes; reaction units containing chemical reagents, namely, reaction systems for medical testing purposes; Nanopore sensing devices for medical and veterinary purposes.
38.
ALIGNMENT OF TARGET AND REFERENCE SEQUENCES OF POLYMER UNITS
A relationship (30) between a target sequence of polymer units in a target polymer (10) and a reference sequence of polymer units (20) in a reference polymer such as an alignment is determined from a measured target signal (11) comprising signal levels measured by a measurement system from parts of the target polymer (10) ordered along the target sequence. The measured target signal (10) is segmented, and a sequence of target signal symbols (13) is derived, each representing a quantised signal level derived from the signal levels of a respective segment. A sequence of reference signal symbols (23) representing quantised signal levels of a sequence of modelled reference signal levels predicted by a measurement system model to be measured from the reference sequence of the reference polymer (20) by the measurement system is also used. The sequence of target signal symbols (13) is aligned with the sequence of reference signal symbols (23) to derive the relationship (30) between the target sequence and the reference sequence.
The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p.ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
The present invention relates to novel pore monomer conjugates comprising pore monomers and functionalised partner molecules, pore complexes formed from the conjugates and their uses in analyte detection and characterisation.
Provided herein are methods of characterising a target polypeptide as it moves with respect to a nanopore. Also provided are related kits, systems and apparatuses for carrying out such methods.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
42.
BIOCHEMICAL ANALYSIS SYSTEM AND METHOD OF CONTROLLING A BIOCHEMICAL ANALYSIS SYSTEM
A method of controlling a biochemical analysis system for analysing polymers comprising a sequence of polymer units is provided. The system is operable to take successive measurements of a polymer from a sensor element during translocation of the polymer with respect to a nanopore of the sensor element. The method comprises, when a polymer has partially translocated through the nanopore, analysing the measurements taken from the polymer during the partial translocation thereof to determine modification information in respect of a portion of the sequence of the polymer units. The polymer is classified as belonging to one of a set of classes based on the modification information; and the system is operated to reject the polymer or continue taking measurements from the polymer based on the class to which the polymer unit is classified as belonging.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
09 - Appareils et instruments scientifiques et électriques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Chemical diagnostic reagents for scientific testing and scientific research use; chemicals, namely, reagents for use in automated test systems for scientific testing, scientific research and industrial use; chemicals, namely, reagents for analytical tests for scientific testing, scientific research and industrial use; chemicals, namely, reagents for industrial use; chemicals, namely, reagents for use in molecular biology, biotechnology, microbiology, and bacteriology; chemicals, namely, reagents for nucleic acid amplification for scientific testing, scientific research and industrial use; chemicals, namely, reagents for use in forensic analysis for scientific testing and scientific research use; chemicals, namely, reagents for blood grouping; reagents for use in environmental testing and environmental analysis; reagents for testing water and other liquids for scientific research and industrial purposes; chemicals, namely, deoxyribonucleic acid, single stranded and double stranded deoxyribonucleic acid for use in scientific laboratory tests and experiments; chemicals, namely, ribonucleic acid; peptide nucleic acids for use in scientific tests and experiments; locked nucleic acids for use in scientific tests and experiments; nucleic acids for use in scientific tests and experiments; oligonucleotides for use in scientific testing and research use; oligosaccharides for scientific testing and research use; proteins for scientific testing and research use; peptides for scientific testing and research use; chemicals, enzymes and reagents for use in genome, DNA, RNA and nucleic acid synthesis for scientific research, scientific testing and industrial use; endonucleases, polymerases, ligases, glycosylases for use in scientific tests and experiments; nucleotides, pyrimidines, purines for use in scientific tests and experiments; amino acids for use in scientific tests and experiments; saccharides for use in scientific tests and experiments; reagents for use in nucleic acid characterisation and nucleic acid sequencing for use in scientific tests and experiments; reagents for protein identification and analysis for use in scientific tests and experiments; reagents for formation of amphipathic membranes for use in scientific tests and experiments; buffer solutions for use in scientific tests and experiments; chemicals, namely, surfactants for use in scientific tests and experiments; silicone oils for use in scientific tests and experiments; lytic enzymes including lysozyme, lyticase and cellulose for use in scientific tests and experiments; proteases for use in scientific tests and experiments; nucleases, including RNase, DNase and restriction enzymes for use in scientific tests and experiments; chaotropes, including guanidine-HCl and guanidinium thiocyanate for use in scientific tests and experiments; proteins for use in analytical tests for scientific testing and scientific research purposes; all the aforesaid other than for medical or veterinary purposes Chemical diagnostic Reagents for use in automated test systems for medical and veterinary use; chemicals, namely, reagents for medical and veterinary testing and research purposes for use in blood grouping, molecular biology, bacteriological, biotechnology and microbiological analysis, including reagents for formation of amphipathic membranes, buffer solutions, non-aqueous solvents, surfactants; chemicals, namely, reagents for medical and veterinary diagnosis and medical and veterinary research; chemicals, namely, reagents for laboratory tests for medical or veterinary purposes; medical and veterinary diagnostic reagents, namely, nucleic acid amplification reagents for veterinary, medical, medical research or forensic use; reagents for immunoassay analysers for medical and veterinary use; chemical diagnostic reagents for testing for susceptibility to allergies; reagents for use in nucleic acid characterisation and nucleic acid sequencing for medical and veterinary diagnostic use; reagents for protein identification and analysis for medical and veterinary diagnostic purposes; reagents for formation of amphipathic membranes for medical and veterinary diagnostic use; proteins for use in tests for medical or veterinary diagnostic purposes Downloadable software for running an e-commerce platform, namely, software that allows users to perform electronic business transaction over the Internet; cloud computing software for running an e-commerce platform, namely, software that allows users to perform electronic business transaction over the Internet; downloadable software for use in analysing, transmitting and receiving data via a global communications network; cloud computing software for use in analysing, transmitting and receiving data via a global communications network; downloadable software for developing, running and communicating with software on data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; computer operating software; computer operating system software; computer operating system software for data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices; laboratory equipment, namely, micro-array chips; electronic sensing apparatus for analysing chemical, biochemical, biological, bacteriological and microbiological test samples for scientific testing purposes; scientific testing apparatus and instruments, namely, optical or electrochemical readers for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples and the measurement of ions; portable apparatus and instrument, namely, optical or electrochemical readers for biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular test samples and measurement of ions; scientific instruments, namely, sequencing devices for use in DNA, RNA, protein and polynucleic acid sequencing; computer software and optical or electrochemical readers for the analysis of polynucleic acid sequences; scientific apparatus and instruments for the analysis of nucleic and polynucleic acids; scientific apparatus and instruments for the analysis of proteins; apparatus and instruments for analysis of genetic information, genomic information and proteomic information, namely, automated instruments for detecting the presence, properties and identity of analytes, comprising cartridges, microarrays, silicon chip based arrays, ASICS (application specific integrated circuits), and networked computer hardware and software for performing and controlling the analysis, and for processing, displaying and storing the information obtained from the analysis; computer programs, computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; scientific apparatus and instruments; electrowetting-on-dielectric (EWOD) devices; scientific apparatus and instruments for automating the extraction and preparation of chemical, biochemical, biological, bacteriological and microbiological test samples test samples; scientific apparatus, devices and instruments for synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific research and scientific testing purposes; data processing apparatus, devices and instruments; scientific apparatus, devices and instruments for identifying, measuring, analysing, sequencing or synthesising small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific research and scientific testing purposes; portable computer hardware and data processing apparatus, all for the analysis and recordal of scientific data; electronic display interfaces for the display of information obtained from scientific equipment; silicon chips; DNA chips; biosensor chips for scientific research and scientific testing use; cartridges namely, cartridges for test samples and cartridges for use with concentrates and solutions in solid or liquid form for use in biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis Medical and veterinary testing and diagnostic apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; electrowetting devices for medical and veterinary purposes; apparatus and instruments for automating the extraction and preparation of test samples for medical, and veterinary purposes; portable medical and veterinary testing and diagnostic apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable diagnostic apparatus for medical or veterinary purposes for pregnancy testing, and for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; portable testing apparatus for medical or veterinary purposes for detecting pathogenic, infectious, psychiatric, oncogenic, metabolic, genetic, respiratory, genitourinary, digestive, inflammatory, autoimmune, reproductive, hepatic, central nervous system, degenerative and cardiovascular diseases or conditions; cartridges for test samples, for medical purposes; reaction units containing chemical reagents, namely, reaction systems for medical testing purposes; Nanopore sensing devices for medical and veterinary purposes Scientific and technological services and research and design relating thereto, namely, consultation, research and design services relating to automated systems and instruments for direct electrical detection and analysis of molecules for use in the field of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defence; industrial research services in the fields of genomics, protein analysis, molecular analysis, life sciences, healthcare, security and defence; scientific research in the fields of chemical, biochemical, industrial, biological, bacteriological, medical and veterinary science; biological, chemical, biochemical, viral, bacteriological, DNA, RNA, protein, polynucleic acid and molecular analysis services, namely, biological, chemical, biochemical, bacteriological DNA, RNA, protein, polynucleic acid and molecular analysis for scientific testing and scientific research purposes; scientific and technological services, namely, to the synthesising chemical compounds, small molecules, genomes, DNA, RNA or other nucleic acids, oligosaccharides, proteins and peptides for scientific purposes and medical research purposes; scientific research for medical or veterinary purposes; DNA and RNA and polynucleic acid sequencing services for scientific research purposes; protein sequencing services for scientific research purposes; design and development of instruments and apparatus for biological, chemical, biochemical, DNA, RNA, protein and molecular analysis; laboratory testing services, namely, the analysis of DNA and polynucleic acids for scientific and scientific research purposes; DNA and polynucleic acid testing services, namely, the analysis of DNA and polynucleic acids for scientific and scientific research purposes; genome sequencing services for scientific testing and scientific research purposes; analysis and evaluation of data, in the fields of fields of biology, chemistry, biochemistry, bacteriology, DNA and RNA for scientific testing and scientific research purposes; computerised analysis of data; overseeing, monitoring and managing scientific and medical experiments and tests scientific testing and scientific research purposes; overseeing, monitoring and managing the analysis and evaluation of scientific and medical data for scientific research and scientific testing purposes; design of scientific experiments; analysis of data generated by scientific and medical experiments and tests for scientific research and scientific testing purposes; biological, bacteriological, chemical, biochemical, DNA, RNA, protein, polynucleic acid and molecular analysis and evaluation services for scientific research and scientific testing purposes; DNA and RNA and polynucleic acid and protein sequencing, analysis and evaluation services for scientific research and scientific testing purposes; genome sequencing services and the analysis and evaluation of data resulting therefrom for scientific testing and scientific and clinical research purposes; provision of reports relating to scientific and medical experiments, the results of scientific and medical experiments and tests, the analysis and evaluation of scientific and medical data, and data generated by scientific and medical experiments and tests all in the fields of chemical, biochemical, biological, bacteriological, genomic and molecular analysis for scientific testing and scientific research purposes; scientific research and development services in the fields of chemical, biochemical, biological, bacteriological, genomic and molecular analysis for scientific testing and scientific research purposes; technical data analysis and evaluation services for scientific research and scientific testing purposes;; application service provider (ASP) services featuring computer software applications installed or downloaded onto data processing apparatus, scientific apparatus, medical and veterinary apparatus, computers, mobile phones, tablet computers and handheld mobile digital electronic devices for use in the analysis and recordal of data obtained from scientific experiments and tests
C07K 14/35 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de bactéries provenant de Mycobacteriaceae (F)
A nanopore device and a method of controlling a nanopore device for analysing a polymer, the nanopore device comprising at least one sensor element, the sensor element comprising a nanopore and a sensor, the method comprising: translocating a polymer through the nanopore; generating a series of measurements using the sensor as the polymer translocates through the nanopore; comparing the series of measurements to a reference data to determine a measurement of similarity; operating the nanopore device to eject the polymer from the nanopore if the measure of similarity is determined to be below a threshold value; and determining whether the polymer has been successfully ejected from the nanopore by analysing a further set of measurements taken by the sensor.
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
A method of amplifying a target polynucleotide, comprising: providing a template polynucleotide comprising a 5′ hairpin, a target polynucleotide and a 3′ hairpin, wherein the 5′ hairpin comprises one or more non-canonical nucleotides and contacting the template polynucleotide with a polymerase and canonical nucleotides, wherein the polymerase extends, using the canonical nucleotides, the target polynucleotide from its 3′ end to form a first extended polynucleotide comprising the 5′ hairpin at its 5′ end and the complement of the 5′ hairpin at its 3′ end, wherein the complement of the 5′ hairpin forms a 3′ hairpin; and the polymerase extends the first extended polynucleotide from its 3′ end to form a second extended polynucleotide comprising the 5′ hairpin at its 5′ end and the complement of the 5′ hairpin at its 3′ end, wherein the complement of the 5′ hairpin forms a 3′ hairpin.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p.ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C12N 15/11 - Fragments d'ADN ou d'ARN; Leurs formes modifiées
C12N 15/66 - Méthodes générales pour insérer un gène dans un vecteur pour former un vecteur recombinant, utilisant le clivage et la ligature; Utilisation de linkers non fonctionnels ou d'adaptateurs, p.ex. linkers contenant la séquence pour une endonucléase de restriction
A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising:
(i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein:
(a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and
(b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides;
(ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore;
(iii) measuring current blockades having a duration within a defined window, wherein:
(a) the one or more non-natural nucleotides present in the hybridisation region of the probe increase or decrease the duration of the current blockade due to the probe hybridised to its target polynucleotide such that the proportion of current blockades that occur within the window due to the interaction of the hybridised probes with the pore is increased compared to when the corresponding one or more natural nucleotides are present in the hybridisation region; and
(b) each hybridised probe gives rise to a current blockade indicative of that probe; and
(iv) correlating the measured current blockades with the probes, thereby determining the presence, absence or amount of the two or more target polynucleotides in the sample.
C12Q 1/6825 - Détecteurs faisant intervenir la détection d’acides nucléiques
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes
Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.
Devices for improved nanopore sensing are described. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. The structure can include an array of nanopore structures, each nanopore structure comprising a passage for fluid connection through the structure between the analyte reservoir and outlet chamber. Control terminals can be arranged for applying a control signal to alter the electrical potential difference across that nanopore structure. Some embodiments include an electronic circuit configured to detect a signal from an electrical transduction element at each nanopore structure. Additional structural features and methods of operating and making the devices are described.
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 27/12 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'impédance en recherchant la résistance d'un corps solide dépendant de la réaction avec un fluide
53.
METHOD OF DETERMINING THE PRESENCE OR ABSENCE OF A
TARGET ANALYTE COMPRISING USING A REPORTER
POLYNUCLEOTIDE AND A TRANSMEMBRANE PORE
The invention relates to a method of determining the presence or absence of a target analyte in a sample. The method comprises immobilising any target analyte present in the sample on a surface; contacting the surface with: (i) a first detection agent that binds specifically to the target analyte; and (ii) a reporter polynucleotide, wherein the reporter polynucleotide is bound to, or binds to, the first detection agent; and contacting a transmembrane pore with any reporter polynucleotide that has been immobilised on the surface, wherein the reporter polynucleotide is immobilised on the surface by binding of the first agent to the target analyte, and using the transmembrane pore to detect the reporter polynucleotide, thereby determining the presence or absence of the target analyte in the sample.
G01N 33/543 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins
C07K 14/35 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de bactéries provenant de Mycobacteriaceae (F)
The present invention relates to novel pore monomer conjugates, pore complexes formed from the conjugates and their uses in analyte detection and characterisation.
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
B82Y 5/00 - Nanobiotechnologie ou nanomédecine, p.ex. génie protéique ou administration de médicaments
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p.ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
C07K 14/00 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés
C07K 14/475 - Facteurs de croissance; Régulateurs de croissance
The present invention relates to novel pore monomer conjugates, pore complexes formed from the conjugates and their uses in analyte detection and characterisation.
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
B82Y 5/00 - Nanobiotechnologie ou nanomédecine, p.ex. génie protéique ou administration de médicaments
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p.ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
C07K 14/00 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés
C07K 14/475 - Facteurs de croissance; Régulateurs de croissance
The present invention relates to novel pore monomer conjugates, pore complexes formed from the conjugates and their uses in analyte detection and characterisation.
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
B82Y 5/00 - Nanobiotechnologie ou nanomédecine, p.ex. génie protéique ou administration de médicaments
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p.ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
C07K 14/00 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés
C07K 14/475 - Facteurs de croissance; Régulateurs de croissance
Aspects of the disclosure relate to protein pore complexes and their uses in analyte detection and characterisation. The disclosure is based, in part, on nanopore complexes formed by CsgG-like pores and one or more auxiliary proteins, which form one or more channel constrictions in the nanopore complex. In some embodiments, the one or more auxiliary protein is a fusion protein. The disclosure further relates to methods for design of auxiliary proteins and production of the nanopore complexes, and for use in molecular sensing and analyte sequencing applications.
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
B82Y 5/00 - Nanobiotechnologie ou nanomédecine, p.ex. génie protéique ou administration de médicaments
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p.ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
C07K 14/00 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés
C07K 14/475 - Facteurs de croissance; Régulateurs de croissance
Provided herein are methods of characterising a target polypeptide as it moves with respect to a nanopore. Also provided are related kits, systems and apparatuses for carrying out such methods.
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
C12N 15/11 - Fragments d'ADN ou d'ARN; Leurs formes modifiées
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
60.
METHOD FOR ATTACHING ONE OR MORE POLYNUCLEOTIDE BINDING PROTEINS TO A TARGET POLYNUCLEOTIDE
The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also related to new methods of characterising target polynucleotides.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12N 9/00 - Enzymes, p.ex. ligases (6.); Proenzymes; Compositions les contenant; Procédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/6834 - Couplage enzymatique ou biochimique d’acides nucléiques à une phase solide
The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.
C12N 15/115 - Aptamères, c. à d. acides nucléiques liant spécifiquement une molécule cible avec une haute affinité sans s'y hybrider
G01N 33/53 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet
G01N 33/543 - Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
62.
METHOD OF TARGET MOLECULE CHARACTERISATION USING A MOLECULAR PORE
The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled form the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.
The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.
A method of calibrating a nanopore array device is described. The nanopore array device comprises an array of nanopore channels, each nanopore channel formed in a membrane separating two ionic solutions. The nanopore channel connects the ionic solutions and the device further comprises a thermal control component for adjusting the temperature of the array of nanopore channels. The method comprises the steps of measuring signals indicative of ion flow through the nanopore channels; analysing the measurement signals and comparing to a reference value; and adjusting the thermal control component to regulate the temperature of the array of nanopore channels based upon the comparison.
Aspects of the disclosure relate to methods for extracting and/or purifying nucleic acids from biological samples. The disclosure is based, in part, on methods comprising contacting DNA in a sample with a polyhedral, rigid substrate under conditions under which the nucleic acids interact (e.g., adsorb or bind) with the substrate to form aggregates, and eluting isolated or purified DNA from the substrate after washing or other sample processing techniques. In some embodiments, methods described by the disclosure result in less sheared isolated or purified DNA relative to previously employed substrates. The resulting isolated nucleic acids may be used for sequencing, for example ultra-long read DNA sequencing.
A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sense a fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.
The present invention relates to modified Dda helicases which can be used to control the movement of analytes such as polynucleotides. The modified Dda helicases are used in analyte detection and characterisation. The present invention also relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore.
C07K 14/35 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de bactéries provenant de Mycobacteriaceae (F)
The disclosure relates to The disclosure relates to methods of attaching a nucleic acid adapter to a target polynucleotide, and methods of preparing a nucleic acid library, using a topoisomerase activated adapter. The disclosure also relates to methods of producing adapted PCR amplicons, and kits for carrying out the methods of the disclosure.
A microfluidic device comprises: a sensor provided in a sensing chamber; a liquid inlet and liquid outlet connecting to the sensor chamber for respectively passing liquid into and out of the sensing chamber and; a sample input port in fluid communication with the liquid inlet; a liquid collection channel downstream of the sensing chamber outlet; a flow path interruption between the liquid outlet and the liquid collection channel, preventing liquid from flowing into the liquid collection channel from upstream; a buffer liquid filling from the sample input port to the sensing chamber, and filling the sensing chamber and filing from the liquid outlet to the flow path interruption; an activation system operable to complete the flow path between the liquid outlet and the liquid collection channel such that the sensor remains unexposed to gas or a gas/liquid interface.
The invention provides a method of selectively characterising polynucleotides of a desired property, such as length, using a nanopore, based on the translocation of the polynucleotide through or across the nanopore. Kits and systems for use in such methods are also provided. The methods of the invention are particularly suitable for sequencing polynucleotides such as DNA.
A method of calibrating a nanopore array device. The nanopore array device comprising: a common electrode, an array of sensing elements each comprising a sensing electrode and a nanopore channel, and an ionic solution in contact with the common electrode and the array of sensing electrodes, the ionic solution providing electrical communication between the common electrode and each of the array of sensing electrodes via the corresponding array of nanopore channels. The method comprises the steps of: applying a two or more test signals across the nanopore channels between the common electrode and array of sensing electrode measuring a corresponding current or voltage value associated with each sensing electrode for each test signal, determining an offset value for each sensing electrode from the measured current or voltage signals, and calculating a general offset value from the determined offset values, and applying a calibrated signal between the common electrode and sensing electrodes; wherein the calibrated signal is adjusted by the general offset value.
The disclosure relates to novel primers, and their use to detect the presence of drug resistance mutations in a sample from a subject with suspected or confirmed Tuberculosis.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
74.
MODIFIED NANOPORES, COMPOSITIONS COMPRISING THE SAME, AND USES THEREOF
Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.
There are provided apparatuses for controlling insertion of a membrane channel into a membrane, comprising: a first bath for holding a first liquid in contact with a first surface of the membrane; a second bath for holding a second liquid in contact with a second surface of the membrane, wherein the membrane separates the first and second liquids; a first electrode configured to contact the first liquid; a second electrode configured to contact the second liquid; and a driving circuit configured to apply a potential difference across the membrane via the first and second electrodes to promote insertion of a membrane channel into the membrane from the first liquid or the second liquid. Various configurations of the driving circuit are described that allow effective promotion of membrane channel insertion while reducing the risk of damage to the membrane. Corresponding methods are also described.
The invention relates to a process for producing a substrate comprising an aperture, which process comprises providing a substrate which comprises a solid-state membrane and a chemical surface modification on a first surface of the solid-state membrane; and forming an aperture through the chemical surface modification and the solid-state membrane. The invention also relates to a substrate comprising a chemical surface membrane and an aperture, a sensor comprising such a substrate and an apparatus comprising such a substrate.
The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.
An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.
There is disclosed a nanopore support structure comprising a wall layer comprising walls defining a plurality of wells, and overhangs extending from the walls across each of the wells, the overhang defining an aperture configured to support a membrane suitable for insertion of a nanopore. There is further disclosed a nanopore sensing device comprising a nanopore support structure, and methods of manufacturing the nanopore support structure and the nanopore sensing device.
The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
Provided herein is a method of moving a double-stranded polynucleotide with respect to a nanopore using a motor protein. The method allows a portion of the polynucleotide to be interrogated by the pore multiple times. Also provided are polynucleotide adapters and kits comprising such adapters. The methods find use in characterising polynucleotides, for example in sequencing.
An apparatus for supporting an array of layers of amphiphilic molecules, the apparatus comprising: a body, formed in a surface of the body, an array of sensor wells capable of supporting a layer of amphiphilic molecules across the sensor wells, the sensor wells each containing an electrode for connection to an electrical circuit, and formed in the surface of the body between the sensor wells, flow control wells capable of smoothing the flow of a fluid across the surface.
The invention relates to new methods of moving helicases past spacers on polynucleotides and controlling the loading of helicases on polynucleotides. The invention also relates to new methods of characterising target polynucleotides using helicases.
The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.
Provided herein are methods of characterising a target polypeptide as it moves with respect to a nanopore. Also provided are related kits, systems and apparatuses for carrying out such methods.
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
The present invention relates to novel Rhodococcus porin monomers and pores comprising the monomers, and methods of characterising analytes, such as polypeptides and polynucleotides, using the pores.
C07K 14/195 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de bactéries
C12Q 1/00 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismes; Compositions à cet effet; Procédés pour préparer ces compositions
G01N 33/50 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique
A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.
A measurement signal measured from a polymer during translocation of the polymer with respect to a nanopore is analysed using an input sequence estimate of the sequence of polymer units of the polymer, and a mapping between the measurement signal and the input sequence estimate. In particular, a sequence slice derived from a slice of the input sequence estimate around a subject polymer unit in the sequence of polymer units, and a signal slice of the measurement signal mapped to the sequence slice by the mapping, are supplied as inputs to a slice machine learning system that provides an output representing an estimate of the identity of the subject polymer unit.
Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.
A fluidic device, such as an electrowetting-on-dielectric device, is provided. The device comprises a fluid chamber containing apolar fluid. The fluid chamber comprises an input port and a channel fluidly connected to the input port. The input port is formed with an opening to receive a pipette tip and impart a droplet of polar fluid into the channel. The insertion of the pipette tip into the fluid chamber causes displacement of the apolar fluid in the fluid chamber such that when the pipette tip is retracted from the fluid chamber, apolar fluid displaces the polar fluid from the surface of the pipette tip to form a droplet of polar fluid surrounded by apolar fluid in the channel of the fluid chamber.
The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p.ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
The invention relates generally to a method of detecting and/or analysing target polymers, especially target polynucleotides, using a biological pore. The invention also relates to a novel system for carrying out the method. The method has many uses. In particular, the method may be used for diagnosis, detection of polymorphisms and V(D)J repertoire analysis.
The invention relates generally to a method of detecting and/or analysing target polymers, especially target polynucleotides, using a biological pore. The invention also relates to a novel system for carrying out the method. The method has many uses. In particular, the method may be used for diagnosis, detection of polymorphisms and V(D)J repertoire analysis.
Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.
The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.
A61K 38/00 - Préparations médicinales contenant des peptides
B82Y 15/00 - Nanotechnologie pour l’interaction, la détection ou l'actionnement, p.ex. points quantiques comme marqueurs en dosages protéiques ou moteurs moléculaires
C07K 14/00 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés
The invention relates to mutant forms of Cytotoxin K. The invention also relates to methods of analyte detection and characterisation using Cytotoxin K, together with devices and kits for carrying out such methods.
C07K 14/32 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de bactéries provenant de Bacillus (G)
98.
ADAPTORS FOR NUCLEIC ACID CONSTRUCTS IN TRANSMEMBRANE SEQUENCING
The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p.ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
Described herein is a method of sequencing, comprising: splitting an asymmetrically tagged library into a plurality of subsamples, tagging the adaptor-ligated DNA in the sub-samples with sequence tags that identify the subsamples, optionally pooling the sub-samples, sequencing polynucleotides from each of the tagged sub-samples, or copies of the same, to produce sequence reads each comprising: i. a sub-sample identifier sequence and ii. the sequence of at least part of a fragment in the sample, wherein some of the sequence reads are derived from the top strand of a fragment in the sample and some of the sequence reads of are derived from the bottom strand of the same fragment.
The invention relates to mutant forms of Cytotoxin K. The invention also relates to methods of analyte detection and characterisation using Cytotoxin K, together with devices and kits for carrying out such methods.
C07K 14/32 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant de bactéries provenant de Bacillus (G)
C12N 15/01 - Préparation de mutants sans introduction de matériel génétique étranger; Procédés de criblage à cet effet
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 33/68 - Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique faisant intervenir des protéines, peptides ou amino-acides
