The present invention relates to an apparatus and method for efficiently isolating or purifying a target substance from a biological sample by using a sample comprising magnetic particles and an apparatus comprising a magnet. In the apparatus according to the present invention, a transfer unit, together with a rotation motion, moves up and down in a reagent inside a chamber, and thus the magnetic particles and a buffer are sufficiently mixed, so that even when a small amount of elution buffer is used, the mixing of the magnetic particles and the buffer can be attained. In addition, since the reagent inside the chamber is mixed by fast and strong rotation, the process does not proceed while the magnetic particles remain at the corner of the chamber or adhere to a wall portion thereof, with the results that the extraction efficiency of the target substance can be maximized and the efficiency of subsequence PCR analysis can also be improved.
The present invention relates to: an inlet/outlet structure of a microfluidic chip optimized to seal an inlet/outlet thereof by using a UV-curable sealing material; a microfluidic chip having the inlet/outlet structure; and a method for sealing the inlet/outlet of the microfluidic chip by using a UV-curable sealing material. According to the present invention, it is possible to provide a semi-permanent sealing method by which an inlet/outlet of a microfluidic chip can be less prone to be contaminated with a fluid specimen or a harmful reagent and can be hermetically sealed using convenient equipment even in the absence of high temperature/high pressure conditions. Further, the application of the inlet/outlet structure of a microfluidic chip and the sealing method therefor according to the present invention can suppress the generation of bubbles upon predetermined reactions in the microfluidic chip, thus improving accuracy and deviations in reaction results. In addition, an auxiliary tool, such as a chip case, is not required and a manual operation is minimized or fully removed, thus making it possible to apply a fully automated system.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
3.
MULTIPLEX PCR CHIP AND MULTIPLEX PCR DEVICE COMPRISING SAME
In accordance with an embodiment of the present invention, provided are a multiplex PCR chip, and a multiplex PCR device comprising the same. The chip comprises: a plurality of probes for use in hybridization reactions, which are specifically hybridized with different sequences of a plurality of different nucleic acid molecules in order to detect the nucleic acid molecules simultaneously, and are arranged spaced apart from each other; and a plurality of probe coupling parts which are arranged on the inner surface of the multiplex PCR chip, form a pore structure so as to increase the contact area between the probes and the nucleic acid molecules, and thereby allow the probes to be coupled to the pore structure, wherein the probes may be characterized in that a fluorescent substance and a fluorescence-inhibiting substance are combined at the terminal or the middle of a base sequence, respectively.
Disclosed according to one embodiment of the present invention are a nucleic acid extraction apparatus and an operation method therefor. The apparatus may comprise: a first rack having multiple sample tube accommodation units in a circle; a second rack having multiple elution tube accommodation units in a circle on the outside and a cleaning solution accommodation unit at the center, wherein at least a part of the cleaning solution accommodation unit protrusively extends outwards so as to alternate with multiple elution tubes; a main body having the first and second racks disposed such that the first rack is positioned over the second rack; a rotational operation unit for rotating each of the first and second racks; a dispenser for dispensing each of a cleaning solution and an eluate into sample tubes; and a pressing unit for pressing the inside of the sample tubes.
One embodiment of the present invention relates to a PCR apparatus comprising a repeated sliding means and a PCR method using same. According to the present invention, the throughput of samples can be increased by simultaneously, rapidly, and accurately performing a PCR on the large number of samples through repeated thermal contact between a thermal block having two or more heaters disposed therein and a PCR chip having two or more reaction chambers disposed therein. In addition, the present invention is capable of: significantly improving PCR yield by preventing radial heat distribution generated by the individual heaters and the consequent nonuniform thermal overlap between the adjacent heaters; significantly contributing to the miniaturization and integration of the apparatus by not requiring a separate temperature control means; furthermore, simultaneously and rapidly amplifying multiple nucleic acid samples by using the thermal block having heater units repeatedly disposed therein and a plate-shaped PCR unit; and checking the process of nucleic acid amplification in real time by measuring sequential optical signals or electrochemical signals.
According to one embodiment of the present invention, a sealing device of a microfluidic chip and an operation method therefor are provided. The device can comprise: a support part in which the microfluidic chip is arranged; and a heat sealing part applying heat to an inlet part and an outlet part of the microfluidic chip so as to seal the inlet part and the outlet part.
According to one embodiment of the present invention, a multiplex PCR device is disclosed. The multiplex PCR device comprises a multiplex PCR chip simultaneously carrying a plurality of mutually different nucleic acid molecules, and the invention may be characterised in that, attached spaced apart from each other on the multiplex PCR chip, there are a plurality of probes used for hybridization reactions whereby hybridization takes place specifically with mutually different amplified sequences of the nucleic acid molecules.
According to embodiments of the present invention, a microfluidic chip, a manufacturing method therefor and an analysis device using the same are provided. The microfluidic chip comprises: a substrate comprising an inflow part through which a fluid flows in, a fluid channel through which the fluid moves and an outflow part through which the fluid flows out; and a film attached to the substrate to protect at least one of the inflow part, the outflow part and the fluid channel from the outside, wherein the inflow part and the outflow part are implemented by penetrating through the surface of the substrate, and the fluid channel can be implemented by being sunk from the surface of the substrate.
The present invention relates to a microfluidic chip and a real-time analysis device using same, and more specifically, to a microfluidic chip and a real-time analysis device using same capable of securing reliability of measurement results by appropriately preventing reduction of optical signal sensitivity due to bubbles included in a fluid. According to one embodiment of the present invention, the microfluidic chip is provided. The microfluidic chip comprises: at least one reaction chamber which comprises at least one optical measuring area, and in which a random reaction of a fluid received therein takes place; and a bubble eliminating portion comprising a light transmitting material which protrudes from an the inner surface of an upper part of the microfluidic chip toward the inside of the reaction chamber, to prevent bubbles included in the fluid from being included in the optical measuring area.
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
G01N 33/48 - Biological material, e.g. blood, urine; Haemocytometers
One embodiment of the present invention relates to a PCR device provided with a unidirectional sliding means and a PCR method using same. According to the present invention, the amount of samples treated can be increased by simultaneously, rapidly, and accurately performing a PCR on a large number of samples through sequential thermal contact between a thermal block in which at least two heaters are repeatedly arranged and a PCR chip in which at least two reaction chambers are repeatedly arranged. Also, the present invention is capable of: significantly improving PCR yield by preventing radial heat distribution generated by each heater and consequent non-uniform thermal overlap between adjacent heaters; significantly contributing to size reduction and integration of the device by not requiring a separate temperature control means; simultaneously and rapidly amplifying multiple nucleic acid samples by using the thermal block in which heater units are repeatedly arranged and a plate-shaped PCR reacting portion; and confirming a nucleic acid amplifying step in real time by measuring continuous optical signals or electrochemical signals.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
11.
ULTRA-HIGH SPEED AND REAL-TIME PCR DEVICE ON BASIS OF LAB-ON-A-CHIP FOR DETECTING FOOD POISONING BACTERIA OF AGRICULTURAL FOOD, AND FOOD POISONING DETECTION METHOD USING SAME
The present invention relates to an ultra-high speed real-time PCR device on the basis of a lab-on-a-chip for detecting food poisoning bacteria of agricultural food and a food poisoning detection method using the same. The present invention can provide a micro PCR chip which can simultaneously accommodate a plurality of small-volume samples and concurrently secure maximum thermal contact efficiency with a heating block so as to secure rapid results, and accurately measure an optical signal emitted from a nucleic acid amplification product even without any separate filtering or processing. Further, on the basis of the PCR chip, the present invention can provide a real-time PCR device which can rapidly obtain a nucleic acid amplification result of which the reliability is secured even without a complicated light-signal measuring module.
A cell and solution condition screening system is disclosed. The cell and solution condition screening system is characterized by comprising: a stage module having a concentration gradient chip for cell culturing arranged on the upper end, the stage module adjusting the position of the concentration gradient chip in at least one direction of horizontal and vertical directions; and an optical module for measuring cells cultured on the concentration gradient chip and the culturing condition of the concentration gradient chip.
The present invention provides a minute fluid cell chip, a method for culturing a cell using the same, and an apparatus for analyzing a cell image using the same. The minute fluid cell chip includes: a plurality of inlet parts for injecting a plurality of fluids respectively; a concentration gradient minute channel connected to the plurality of inlet parts for continuously diluting the concentration of the fluid; a cell culture chamber connected to the concentration gradient minute channel for culturing a cell; a cell injection part formed on the cell culture chamber for injecting the cell; and a discharge part connected to the cell culture chamber. The minute fluid cell chip has the separate cell injection part in addition to the inlet parts and the discharge part, thus improving the capability of injecting and culturing the cell.
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
KOREA FOOD & DRUG ADMINISTRATION (Republic of Korea)
KOREA POLYTECH SPECIALIZED COLLEAGE INDUSTRIAL COOPERATION CORP. (Republic of Korea)
Inventor
Kim, Sung Woo
Hwang, In Gyun
Chung, Sung Oh
Lee, Soon Ho
Choo, Dong Won
Koh, Young Ho
Koh, Hyung Kon
Kim, Kyung-Mi
Abstract
The present invention relates to a primer set for detecting food poisoning, a PCR apparatus using the same, and a method for detecting food poisoning using the same. The method makes it possible to accurately and quickly check for food poisoning with reduced cost, preventing food poisoning from being spread, and significantly contributing to counteracting it.
An embodiment of the present invention relates to a PCR thermal block with pattern heaters repeatedly arranged and a PCR apparatus including the same, wherein the thermal block with heaters repeatedly arranged prevents radial thermal distribution generated from each heater and irregular thermal overlapping caused thereby between adjacent heaters so as to improve the PCR yield and to eliminate an additional temperature adjustment means, thus making the apparatus small and integrated. Furthermore, the thermal block with heater units repeatedly arranged and the plate-like PCR reaction part are used to simultaneously and quickly amplify a plurality of nucleic acid samples, and to continuously measure the optical signal or electrochemical signal so as to check the process of amplifying the nucleic acid in real time.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
16.
MICROFLUIDIC CHIP FOR EXTRACTING NUCLEIC ACIDS, DEVICE FOR EXTRACTING NUCLEIC ACIDS COMPRISING SAME, AND METHOD FOR EXTRACTING NUCLEIC ACIDS USING SAME
The present invention relates to a microfluidic chip for extracting nucleic acids, a device for extracting nucleic acids comprising same, and a method for extracting nucleic acids using same. According to the present invention, unlike existing devices for extracting nucleic acids and methods for extracting nucleic acids, microminiturization and ultra-high speed can be achieved, and reliable nucleic acid extraction efficiency can be maintained and/or improved.
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
17.
MICRO-PCR CHIP COMPRISING PRIMER SET FOR DETECTING FOOD POISONING, REAL-TIME PCR DEVICE COMPRISING SAME, AND METHOD FOR DETECTING FOOD POISONING USING SAME
One embodiment of the present invention relates to a micro-PCR chip and a real-time PCR device comprising same, and according to the embodiment, a plurality of small samples can be accommodated at once while securing maximum thermal contact efficiency with a thermal block thereby rapidly obtaining a result, the micro-PCR chip capable of accurately measuring optical signals emitted from a nucleic acid amplification product without requiring additional filtering or processing can be provided, and based on this premise, the real-time PCR device capable of rapidly obtaining a nucleic amplification result and of which reliability is secured even without requiring a complicated optical signal measuring module can be provided.
The present invention relates to a primer set for detecting food poisoning, a PCR apparatus using same, and a method for detecting food poisoning therewith, the present invention allowing accurate and rapid determination of infection from food-borne bacteria at an economical cost, thereby preventing the spread of food-borne bacteria, and contributing significantly to taking rapid response measures against same.
An embodiment of the present invention relates to a micro PCR chip and to a real-time PCR device comprising same, whereby it is possible to provide a micro PCR chip which is able to simultaneously accommodate a plurality of small-volume samples and at the same time ensure maximum thermal contact efficiency with a heating block so as to ensure rapid results and which is also able to accurately measure optical signals emitted from nucleic acid amplification products even without any separate filtering or processing, and also whereby, based on this, it is possible to provide a real-time PCR device which is able to rapidly obtain nucleic acid amplification results of guaranteed reliability even without a complicated light-signal measuring module.
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
20.
REAL-TIME PCR DEVICE COMPRISING THERMAL BLOCK IN WHICH HEATER UNITS ARE REPEATEDLY ARRANGED FOR DETECTING ELECTROCHEMICAL SIGNALS AND REAL-TIME PCR METHOD USING SAME
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY (Republic of Korea)
Inventor
Kim, Sung Woo
Park, Hyun Gyu
Lee, Jung Hwan
Lee, Yu Jin
Kim, Duck Joong
Won, Byoung Yeon
Baek, Song Yi
Abstract
One embodiment of the present invention relates to a real-time PCR device comprising a thermal block in which heater units are repeatedly arranged for detecting electrochemical signals, and to a real-time PCR method using same. According to the present invention, a plurality of samples can be analyzed simultaneously at an ultra-high speed through the thermal block in which the heater units are repeatedly arranged and a plate-shaped PCR chip, and a simple module that can easily detect continuous electrochemical signals, which are generated during a nucleic acid amplification process, can significantly contribute to the miniaturization and increased portability of a product.
PCR CHIP COMPRISING THERMAL BLOCK IN WHICH HEATER UNITS ARE REPEATEDLY ARRANGED FOR DETECTING ELECTROCHEMICAL SIGNALS, PCR DEVICE COMPRISING SAME, AND REAL-TIME PCR METHOD USING PCR DEVICE
One embodiment of the present invention relates to a PCR chip comprising a thermal block in which heater units are repeatedly arranged for detecting electrochemical signals, a PCR device comprising same, and a real-time PCR method using the PCR device. According to the present invention, a plurality of samples can be analyzed simultaneously at an ultra-high speed through the thermal block in which the heater units are repeatedly arranged and a plate-shaped PCR chip, and a simple module that can easily detect continuous electrochemical signals, which are generated during a nucleic acid amplification process, can significantly contribute to the miniaturization and increased portability of a product.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
22.
PCR CHIP COMPRISING THERMAL BLOCK IN WHICH HEATER UNITS ARE REPEATEDLY ARRANGED FOR DETECTING ELECTROCHEMICAL SIGNALS, PCR DEVICE COMPRISING SAME, AND REAL-TIME PCR METHOD USING PCR DEVICE
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY (Republic of Korea)
Inventor
Kim, Sung Woo
Park, Hyun Gyu
Lee, Jung Hwan
Lee, Yu-Jin
Kim, Duck Joong
Won, Byoung Yeon
Baek, Song Yi
Abstract
One embodiment of the present invention relates to a PCR chip comprising a thermal block in which heater units are repeatedly arranged for detecting electrochemical signals, a PCR device comprising same, and a real-time PCR method using the PCR device. According to the present invention, a plurality of samples can be analyzed simultaneously at an ultra-high speed through the thermal block in which the heater units are repeatedly arranged and a plate-shaped PCR chip, and a simple module that can easily detect continuous electrochemical signals, which are generated during a nucleic acid amplification process, can significantly contribute to the miniaturization and increased portability of a product.
REAL-TIME PCR DEVICE FOR DETECTING ELECTROCHEMICAL SIGNALS COMPRISING HEATING BLOCK IN WHICH HEATER UNITS ARE REPEATEDLY DISPOSED, AND REAL-TIME PCR METHOD USING SAME
One embodiment of the present invention relates to a real-time PCR device for detecting electrochemical signals, the device comprising a heating block in which heater units are repeatedly disposed, and relates to a real-time PCR method using same, whereby not only is it possible to perform simultaneous ultra-high-speed analysis of a plurality of samples by means of the heat block in which the heater units are repeatedly disposed and a plate-shaped PCR chip but it is also possible to significantly contribute to the microminiaturization and increased portability of products through simple modularisation allowing the easy detection of continuous electrochemical signals generated in the process of nucleic acid amplification.
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY (Republic of Korea)
Inventor
Kim, Sung Woo
Park, Hyun Gyu
Lee, Jung Hwan
Lee, Yu Jin
Kim, Duck Joong
Won, Byoung Yeon
Baek, Song Yi
Abstract
An embodiment of the present invention relates to a real-time polymerase chain reaction (PCR) apparatus and method. According to the apparatus and method, through a PCR chip having a dual block and plate shape, not only can a plurality of samples be analyzed at the same time at an ultrahigh speed, but a simple module capable of easily detecting sequential electrochemical signals generated during a nucleic acid amplification process can be embodied, thereby allowing microminiaturization and portability of products.
An embodiment of the present invention relates to a real-time PCR device and method, whereby not only is it possible to perform simultaneous ultra-high-speed analysis of a plurality of samples via a dual heat block and plate-shaped PCR chip but it is also possible to significantly contribute to the microminiaturization and increased portability of products through simple modularization allowing the easy detection of continuous electrochemical signals generated in the process of nucleic acid amplification.
G01N 27/26 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by using electrolysis or electrophoresis
G01N 27/30 - Electrodes, e.g. test electrodes; Half-cells
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
26.
MULTICHANNEL DEVICE FOR DOWNWARDLY INJECTING FLUID, APPARATUS FOR EXTRACTING NUCLEIC ACID COMPRISING SAME, AND METHOD FOR EXTRACTING NUCLEIC ACID BY USING SAME
One embodiment of the present invention relates to a multichannel device for downwardly injecting a fluid, an apparatus for extracting nucleic acid comprising same, and a method for extracting nucleic acid by using same. According to the present invention, a miniscule amount of the fluid can be rapidly injected into at least one very small inlet portion in equal or different amounts, the miniscule amount of the fluid can be accurately distributed into the at least one inlet portion by a single maneuver by a user, and a fluid injection port that is relatively bigger than the at least one inlet portion can be formed, thereby significantly improving user experience, and nucleic acid extraction reaction time can be significantly reduced, when carrying out a variety of biological reactions which use a micromovement chip having the shape of a thin film, thereby rapidly progressing a series of various biological detection or analysis reactions.
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
27.
PRIMER SET FOR DETECTING FOOT AND MOUTH DISEASE ACCORDING TO SERUM TYPE, PCR DEVICE USING SAME, AND METHOD FOR DETECTING FOOT AND MOUTH DISEASE BY USING SAME
The present invention relates to a device for detecting foot and mouth disease (FMD) according to a serum type, and a method for detecting an foot and mouth disease outbreak by using same, and more specifically, the device for detecting food and mouth disease according to the serum type is for detecting at least one gene selected from a group consisting of: foot and mouth disease virus A (Genbank ID number: NC011450); foot and mouth disease virus O (Genbank ID number: NC004004); foot and mouth disease virus C (Genbank ID number: NC002554), foot and mouth disease virus Asia (Genbank ID number: NC004915); foot and mouth disease SAT 1 (Genbank ID number: NC011451); and foot and mouth disease SAT 2 (Genbank ID number: NC011452). According to the present invention, a foot and mouth disease outbreak can be accurately and rapidly confirmed at a low cost, thereby significantly contributing to the prevention of and rapid response to the spreading of foot and mouth disease.
C12N 15/11 - DNA or RNA fragments; Modified forms thereof
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
MULTICHANNEL DEVICE FOR DISTRIBUTING FLUID, APPARATUS FOR EXTRACTING NUCLEIC ACID COMPRISING SAME, AND METHOD FOR EXTRACTING NUCLEIC ACID BY USING SAME
One embodiment of the present invention relates to a multichannel device for distributing a fluid, an apparatus for extracting nucleic acid comprising same, and a method for extracting nucleic acid by using same. According to the present invention, a miniscule amount of the fluid can be rapidly distributed into equal amounts and injected into at least one very small inlet portion, the miniscule amount of the fluid can be accurately distributed into the at least one inlet portion by a single maneuver by a user, and nucleic acid extraction reaction time can be significantly reduced when carrying out a variety of biological reactions, which use a micromovement chip having the shape of a thin film, thereby rapidly progressing a series of various biological detection or analysis reactions.
One embodiment of the present invention relates to a nucleic acid extracting apparatus, which enables the automated, microminiaturized and ultra-high-speed extraction of nucleic acids, wherein the nucleic acid extraction may not be limited to biological samples such as sputum, blood, cell, urine, saliva and tissue, minimizes consumption of sample solution, and maintains and/or improves reliable nucleic acid extraction.
According to the present invention, a PCR apparatus including an optically transmissive heat block is disclosed. The PCR apparatus according to the present invention can quickly perform a nucleic acid reaction, measure in real time, raise analysis efficiency, and be formed as a miniaturized and portable PCR apparatus which is highly durable.
According to the present invention, a PCR device including two heating blocks which is used for nucleic acid amplification reactions is disclosed. Using the PCR device of the present invention, nucleic acid amplification reactions can be efficiently performed.
According to the present invention, a fluorescence polarization analysis apparatus including a dual light source is disclosed. According to the fluorescence polarization analysis apparatus of the present invention, a target material included in a sample solution can be efficiently analyzed.
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