Abstract

One embodiment of the present invention relates to a PCR device provided with a unidirectional sliding means and a PCR method using same. According to the present invention, the amount of samples treated can be increased by simultaneously, rapidly, and accurately performing a PCR on a large number of samples through sequential thermal contact between a thermal block in which at least two heaters are repeatedly arranged and a PCR chip in which at least two reaction chambers are repeatedly arranged. Also, the present invention is capable of: significantly improving PCR yield by preventing radial heat distribution generated by each heater and consequent non-uniform thermal overlap between adjacent heaters; significantly contributing to size reduction and integration of the device by not requiring a separate temperature control means; simultaneously and rapidly amplifying multiple nucleic acid samples by using the thermal block in which heater units are repeatedly arranged and a plate-shaped PCR reacting portion; and confirming a nucleic acid amplifying step in real time by measuring continuous optical signals or electrochemical signals.

IPC Classes  ?

• C12M 1/38 - Temperature-responsive control

Abstract

An embodiment of the present invention relates to a PCR thermal block with pattern heaters repeatedly arranged and a PCR apparatus including the same, wherein the thermal block with heaters repeatedly arranged prevents radial thermal distribution generated from each heater and irregular thermal overlapping caused thereby between adjacent heaters so as to improve the PCR yield and to eliminate an additional temperature adjustment means, thus making the apparatus small and integrated. Furthermore, the thermal block with heater units repeatedly arranged and the plate-like PCR reaction part are used to simultaneously and quickly amplify a plurality of nucleic acid samples, and to continuously measure the optical signal or electrochemical signal so as to check the process of amplifying the nucleic acid in real time.

IPC Classes  ?

• C12M 1/38 - Temperature-responsive control
• C12Q 1/686 - Polymerase chain reaction [PCR]