GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LIMITED (United Kingdom)
Inventor
Diana, Rafael
Abstract
A sparger assembly (100) for a bioprocessing system (10) includes a base (126) and a plurality of spargers (130) connected to the base, each sparger including a plurality of pores, the plurality of spargers each have a generally cylindrical shape. Each of the plurality of spargers includes a sidewall and a top, which define the cylindrical shape, the sidewall and the top each include a plurality of pores. The pores of the sidewall can be arranged around a circumference of the sidewall at an array of heights. Ridges may also be located on the sidewall above a respective array of pores.
B01F 23/231 - Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
B01F 23/233 - Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
B01F 33/453 - Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements
B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
C12M 1/06 - Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
C12M 1/00 - Apparatus for enzymology or microbiology
B01F 101/44 - Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
2.
APPARATUS, SYSTEM, AND METHOD FOR REDUCING A MINIMUM WORKING VOLUME OF A BIOREACTOR
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LIMITED (United Kingdom)
Inventor
Rao, Nagaraj
Smeltzer, Thomas
Mathapati, Milind
Tracy Beem, Heather
Murugesan, Kandakumar
Balakrishnan, Saravanan
Chattopadhyay, Subhanjan
Abstract
An apparatus (30) for reducing a minimum working volume of a stirred tank (12) includes a body (32) having upper (46) and lower (48) surfaces, and an exterior profile shaped to closely fit within an interior of the stirred tank. The body including an interior portion (34) extending through the body (32) from the upper surface to the lower surface, the interior portion configured to receive and partially surround an impeller (200) within the stirred tank, while allowing rotation of the impeller to agitate a fluid in the vessel when in use. The body further including an opening in a side surface of the body, the opening extending into the interior portion and configured to facilitate removal of fluid (60) from the vessel when the stirred tank is in use, and to be removed from the stirred tank via a drain.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LIMITED (United Kingdom)
Inventor
Murugesan, Kandakumar
Kandoth, Bineesh
Rao, Nagaraj
Abstract
A vessel (24) includes an interior volume (25) containing a liquid, a spiral shaft (28) located within the interior volume, and a rotatable impeller (30). The impeller has an aperture (38) that receives the spiral shaft and allows the impeller to travel bidirectionally along the spiral shaft. When the impeller travels along the spiral shaft, the impeller rotates axially about the spiral shaft to agitate a liquid in the interior volume.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Hamilton, Andrew
Hortua Diaz, Alejandra
Hernandez Vera, Rodrigo
Larsson, Kjell
Nilsson, Erik
Abstract
Provided is a membrane transfer cassette for electroblotting, the cassette comprising a first support panel and a second support panel, at least a region of one or each of the first and second support panels having an interlocking pattern of polygonal apertures. The membrane transfer cassette is particularly useful when electroblotting with large format gels as the design of the cassette increases the exposed surface area between the gel and the membrane while also enhancing the strength of the cassette to support large gels but is also advantageous for any size of gel.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Nunes, Claudia, Trindade
Lorenzetti, Martina
Abstract
The present invention provides for simple and rapid filtering of biological samples, whereby a sample can be analyzed in the same device or a different device. In one preferred example, a membrane filter (12) that is particularly useful for the filtration of samples comprising viruses along with other biological materials that need be separated from the viruses is used. A lateral flow device (10) incorporating a filter membrane/membrane filter (12) is also disclosed.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Castan, Andreas
Ohrvik, Helena
Konduru, Nagaraju
Handa, Ashish
Boddapati, Neelima
Chauhan, Alok, Singh
Abstract
Methods and apparatus for scaling in bioprocess systems are disclosed. An example apparatus for bioprocess scaling includes at least one memory to store instructions, and processor circuitry to execute the instructions to identify at least one operating parameter of a target bioreactor, determine an upper boundary and/or a lower boundary defining a design space for at least one bioreactor process parameter to match at least one target parameter range based on the at least one operating parameter, simulate changes in the at least one target parameter range based on an adjustment to the upper boundary and/or the lower boundary in the design space, and configure the target bioreactor using output obtained from the adjustment to the upper boundary and/or the lower boundary to identify a match between the at least one target parameter range and a user-based input of a target bioprocess parameter value.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LIMITED (United Kingdom)
Inventor
Albagli, Douglas
Hennessy, William A
Abstract
Porous liquid-filtering membranes (102) are provided having a boundary region (303) substantially surrounding a pore region (302) and having greater tear resistance than the pore region (302). In various aspects, a liquid-filtering tear-resistant porous polymeric membrane (102) comprises a pore region (302) comprising a plurality of pores having a minimum dimension of less than (100) microns and a ring-shaped tear- resistant boundary region (303).
B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
B01D 71/64 - Polyimides; Polyamide-imides; Polyester-imides; Polyamide acids or similar polyimide precursors
8.
SYSTEM AND METHOD FOR ASEPTIC SAMPLING AND FLUID ADDITION
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Timmins, Mark, R
Sherman, Matthew
Ali, Yasser
Picardo, Marc
Benoit, Keith
Abstract
A sampling system includes a graduated sampling chamber configured for fluid connection to a sample source, a pump device configured for fluid connection with the sampling chamber, and a sterile air filter intermediate the pump device and the sampling chamber, wherein the pump device is selectively actuatable to draw a volume of fluid from the sample source into the sampling chamber.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Balakrishnan, Saravanan
Murugesan, Kandakumar
Rao, Nagaraj, Raghavendra
Hegde, Shashidhar
Abstract
A sterile foam breaking system (14, 42, 52) includes a foam collector (20) having an opening (26), configured to be disposed in a source (12) which generates foam. The sterile foam breaking system (14, 42, 52) further includes a non-contact type suction unit (23) coupled to the foam collector (20). The non-contact type suction unit (23) is configured to transfer the foam via the opening (26) of the foam collector (20) and break a portion of the foam to generate a first quantity of liquid droplets. The sterile foam breaking system (14, 42, 52) additionally includes a foam breaking unit (28, 44, 54) coupled to the non-contact type suction unit (23). The foam breaking unit (28, 44, 54) is configured to receive remaining portion of the foam and the first quantity of liquid droplets and break the remaining portion of the foam to generate a second quantity of liquid droplets.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Marteleur, Klas
Divekar, Devina
Abstract
The present invention relates to a sample processing bag (10) for use in a tissue disaggregation apparatus (200) for disaggregation of tissue therein. A clamp assembly 60 comprising a base support (70) and a clamp mechanism (72) is configured to retain the sample processing bag (10) adjacent to the base support (70) during a tissue disaggregation operation such that action of the tissue disaggregation apparatus 200 acts on the sample processing bag (10) to disaggregate any tissue therein.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Balakrishnan, Saravanan
Murugesan, Kandakumar
Rao, Nagaraj, Raghavendra
Ouellette, Matthew, D
Abstract
An apparatus (100) for managing gas bubbles (50) in a bioprocessing system (10) includes a body portion (110) having a underside surface (112), and an opening (114) in the body portion (110). The body portion (110) is configured for placement within a bioreactor vessel (12) such that the underside surface (112) of the body portion (110) is disposed in a liquid within the bioreactor vessel (12). The underside surface (112) of the body portion (110) is configured to divert rising gas bubbles (50) in the liquid towards the opening (114).
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Hennessy, William A
Albagli, Douglas
Stankowski, Ralph
Abstract
A biocompatible polymeric membrane includes pores (106) defined between two material layers, where the first membrane material layer (101) includes strips, and the second membrane material (104) binds to each of the plurality of first membrane material layer strips (101) includes a plurality of windows (105) exposing each of the first membrane material strips (101). The biocompatible polymeric filtration membrane comprises pores (106) defined by uniform passages defined by the first membrane material layer strips (101) and the second membrane material layer (104) within each window (105).
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Viswanathan, Aravind, Kumar
Seshadri, Jaisimha
Sandusky, Christopher
S, Suthish, Kumar
Ca, Shanmuganathan
Arumugam, Sasikumar
Abstract
Methods and apparatus for bioprocess monitoring are disclosed. An example apparatus includes a controller to monitor a first bioprocess instrument, a data logger to collect data for the first bioprocess instrument, the collected data including data collected while the first bioprocess instrument is transferred from a first location to a second location, a configurator to configure the first bioprocess instrument to operate in a first mode at the first location and in a second mode at the second location, the first or second mode determined based on a type of processing at the first or second location, and a user interface to display the collected data to a user, the collected data including real-time bioprocess monitoring data, the controller to adjust a setting of the first bioprocess instrument based on the monitoring data, the monitoring data used to maintain controlled environmental conditions within a vessel of the instrument.
G06N 3/00 - Computing arrangements based on biological models
G16B 40/00 - ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G16C 20/10 - Analysis or design of chemical reactions, syntheses or processes
14.
COLLAPSIBLE AGITATOR ASSEMBLY FOR A BIOPROCESSING SYSTEM
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Hashemi, Nasim
Abstract
A mixing system (10) includes a container (20) for containing a fluid, and an agitator assembly (100) disposable in the container (20) for mixing the fluid. The agitator (100) includes an extensible shaft (110) and at least one impeller (116, 118) connected to the extensible shaft (110).
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Yang, Jianjun
Yang, Hongyan
Abstract
The invention relates to the field of microcarrier perfusion culture of adherent cells. Specifically, the present invention relates to a high-density microcarrier retention device for perfusion culture of adherent cells, a microcarrier perfusion culture system for adherent cells containing the device, and methods of use thereof. The retention device of the present invention includes a sedimentation chamber, a pipeline connected to a bioreactor, a microcarrier retention filter membrane, a liquid backflushing device, an air backflushing device, a peristaltic pump and a pipeline connected to a receiver. The device has high efficiency in promoting the separation of microcarriers from cell culture medium and is helpful for perfusion culture of adherent cells and microcarriers. The retention device makes the culture volume in the bioreactor more flexible, can perform perfusion culture of 20%-100% of the maximum culture volume of the bioreactor, and the retention device can be linearly amplified according to the amplification of the bioreactor volume.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Stegner, Elizabeth
Murugesan, Kandakumar
Cherunni, Sairam
Dash, Manas
Bagiyanathan, Prasad
Selvaraj, Shivakumar
Kandoth, Bineesh
Maiya, Nityananda
Brocato, Nicholas
Dhanariya, Chandra, Prakash
Sundaramoorthy, Suresh
Abstract
A component management apparatus for a bioprocessing system includes a frame having a plurality of segments, including at least a first segment and a second segment pivotably connected to the first segment such that at least the second segment is movable between a closed position and an open position, and at least one mounting bracket connected to the frame for connection of a bioprocess component.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Alagur, Sahebagouda
Mohan, Prashanth, Hosabettu
Paul, Praveen
Abstract
The bioprocessing perfusion system (10) includes a bioreactor (12) and a feed flow path (14). A first tangential flow filter (16) is coupled to the bioreactor (12) via the feed flow path (14) and a second tangential flow filter (18) is coupled to the bioreactor (12) via the feed flow path (14). The first tangential flow filter (16) is a microfiltration-type filter and the second tangential flow filter (18) is an ultrafiltration-type filter. The first tangential flow filter (16) and the second tangential flow filter (18) are further coupled to a receiving unit (58) via the permeate flow path (60). The first tangential flow filter (16) and the second tangential flow filter (18) are further coupled to the bioreactor (12) via the retentate flow path (46). A control unit (82) is communicatively coupled to the first feed control device (42), the second feed control device (44), the feed drive unit (40), the first permeate control device (64), the second permeate control device (66), the first retentate control device (48), and the second retentate control device (50).
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Murugesan, Kandakumar
Rao, Nagaraj, Raghavendra
Balakrishnan, Saravanan
Abstract
A device (30) includes a base connector (32) having an opening (33) and an impeller connector (64) coupled to the base connector (32). The impeller connector (64) has a through-passage (66) aligned with the opening (33) of the base connector (32). Further, the device (30) includes a flexible tube (34) having a first end (36) and a second end (40), where the first end (36) of the flexible tube (34) is coupled to the impeller connector (64). Furthermore, the device (30) includes a seal component (38) and an impeller (42) coupled to the second end (40) of the flexible tube (34). Additionally, the device (34) includes an enclosure (46) disposed enclosing the impeller (42), the flexible tube (34), the impeller connector (64), and the base connector (32).
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
Inventor
Tuohey, Colin
Stankowski, Ralph
Abstract
The present invention relates to a bioprocess bag (118) comprising a bag wall defining an enclosed volume for holding biomaterials. The bag wall comprises at least one inlet port (142) and at least one outlet port (146). The bioprocess bag (118) also comprises a tube structure (400) comprising a first opened-end proximate the bag wall and a second distal end, the tube extending into the enclosed volume, and the tube structure (400) comprising a reinforced portion (402) proximate the first opened-end.
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD (United Kingdom)
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA (USA)
Inventor
Goodwin, Paul, C
Bowles, Paul, Anthony
Iyer, Rohin
Gratch, Yarden
Mccartney, Mitch, M
Yamaguchi, Mei, S
Davis, Cristina, E
Abstract
Disclosed is a method for monitoring cell density during cell expansion resulting from a cell culture process in a bioreactor comprising the steps of: a) cultivating cells in a bioreactor culture chamber according to a cell culture process having cell culture parameters; b) during said process, introducing cell culture fluid inputs and generating waste materials; c) determining the intensity of volatile organic compounds (VOCs) and their chemical species in the waste materials; and d) estimating the density or population of cells in the bioreactor based on said determination.