A method of determining whether one or more forms of a nucleic acid analyte are present in a sample. The method includes dissolving an amplification reagent with a first solvent, where the amplification reagent contains oligonucleotides sufficient to amplify and detect a first region of a first form of the analyte, where the first solvent contains one or more oligonucleotides which, in combination with the oligonucleotides of the amplification reagent, are sufficient to amplify and detect a second region of a second form of the analyte, where the one or more oligonucleotides of the first solvent are insufficient to amplify and detect the first or second form of the analyte, and where the first and second regions each comprise a different nucleotide base sequence. A purified form of the sample is contacted with the dissolved amplification reagent, thereby forming an amplification reaction mixture which is exposed to temperature conditions sufficient for amplifying the first and second regions of the first and second forms of the analyte, respectively. It is then determined whether the first and/or second forms of the analyte are present in the sample.
Disclosed are compositions and methods for performing nucleic-acid-based assays to determine the presence or absence of a target nucleic acid in a biological sample. The disclosed methods include the use of one or more oligomers that specifically hybridize to a nucleolin nucleic acid to assess the cellularity of the biological sample. Also disclosed are related composition and methods for assessing the cellularity of a biological sample for use in a molecular diagnostic assay.
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Automated test instruments for in vitro specimen processing for laboratory use; specimen transfer instruments for laboratory use Automated test instruments for in vitro specimen processing for medical use; specimen transfer instruments for medical use
4.
SYSTEM FOR PERFORMING NUCLEIC ACID AMPLIFICATION ASSAYS
An automated system for performing nucleic acid amplification assays on samples provided to the system, where the system includes data input components configured to enable input specifying one or more user-defined assay parameters; data storage media storing a first set of assay parameters, the first set of assay parameters consisting of system-defined parameters, and a second set of assay parameters, the second set of assay parameters including the one or more user-defined parameters; and command input components configured to enable input specifying (i) that a first nucleic acid amplification assay be performed on a first sample in accordance with the first set of assay parameters, and (ii) that a second nucleic acid amplification assay be performed on a second sample in accordance with the second set of assay parameters. The system further includes one or more wash stations, a fluid transfer device, a thermal processing station, and a detection station for detecting the presence or absence of multiple analytes.
A kit includes a vessel having a plasma-treated interior surface, solid composition adhered to the plasma-treated interior surface, and a reconstitution liquid. The solid composition has a mass ranging from about 600 micrograms to about 20 grams and is in the form of a lyophilized pellet adhered to the plasma-treated interior surface. The solid composition is macroscopic in three orthogonal dimensions, such that the solid composition is not a coating and has a length, a width, and a height of greater than 1 mm.
Provided are compositions, kits, and methods that can be used to selectively amplify a first target nucleic acid that is distinguishable from a second target nucleic acid, where the two target nucleic acids have both common and different sequence domains. Structured primers useful in the amplification method include 5' and 3' target-hybridizing sequences separated from each other by a joining region that does not hybridize to the target nucleic acid. Polymerase-based extension of the structured primer depends on specific hybridization of the 5' sequence of the primer, thereby facilitating hybridization and extension of the 3' sequence of the primer.
Provided herein are compositions, kits, and methods for detecting methicillin-resistant Staphylococcus aureus (MRSA) nucleic acids. In some embodiments, the compositions, kits, and methods can be used to detect one or more of type i, ii, iii, iv, v, vi, vii, viii, ix, xii, xiii, xiv, xv, or xxi SCCmec right extremity junction (MREJ) MRSA nucleic acids and one or more of mecA, mecC, and/or an additional S. aureus-specific gene.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
A conveyor assembly for transporting a carrier coupled to a receptacle to a processing station located within a housing of an instrument. The conveyor assembly includes a spur conveyor subassembly and a buffer conveyor subassembly for transporting the carrier from a host conveyor assembly to the spur conveyor subassembly. The spur conveyor subassembly includes (i) a rotatable diverter having at least one recess for receiving and moving the carrier between the buffer conveyor subassembly and the spur conveyor subassembly and (ii) a gripper configured to grasp the carrier and move it from the diverter to the processing position located within the housing of the instrument.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
9.
COMPOSITIONS AND METHODS FOR DETECTION OF VIRAL PATHOGENS IN SAMPLES
This disclosure concerns amplification primers, hybridization assay probes, compositions containing such primers and probes, and associated reagents, kits, and methods, that can be used to analyze samples for the presence of SARS-CoV-2, Influenza A virus, Influenza B virus, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B target nucleic acids.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
A puck for removably supporting a receptacle. The puck includes a plurality of fingers arranged about a vertical axis, where each finger has a contact surface configured to be in contact with a receptacle seated in the puck. One or more springs couple the plurality of fingers, thereby biasing the fingers toward the vertical axis. The puck further includes a supporting disc having (i) a disc sidewall projecting from a base to define a pocket for seating a receptacle, (ii) a plurality of first cavities formed in the base and extending in a direction of the vertical axis, and (iii) a puck passageway extending through opposed portions of the disc sidewall in a direction transverse to and offset from the vertical axis, where each of the fingers is rotatably coupled to the supporting disc at a corresponding first cavity of the plurality of first cavities. A synchronization disc is positioned in the pocket of the supporting disc, where each of the fingers is coupled to the synchronization disc such that the contact surfaces of the fingers move toward and away from the vertical axis in a synchronous manner. The puck additionally includes a retaining ring coupling the plurality of fingers, the supporting disc, and the synchronization disc together.
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
A conveyor assembly for transporting a carrier coupled to a receptacle to a processing station located within a housing of an instrument. The conveyor assembly includes a spur conveyor subassembly and a buffer conveyor subassembly for transporting the carrier from a host conveyor assembly to the spur conveyor subassembly. The spur conveyor subassembly includes (i) a rotatable diverter having at least one recess for receiving and moving the carrier between the buffer conveyor subassembly and the spur conveyor subassembly and (ii) a gripper configured to grasp the carrier and move it from the diverter to the processing position located within the housing of the instrument.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
An automated conveyor assembly for transporting a carrier coupled to processing receptacle from (i) a carrier conveyor assembly to (ii) a processing position within an instrument, where the automated conveyor assembly includes a gripper configured to selectively grasp a carrier and move between (i) a first position and (ii) the processing position in the instrument. The automated conveyor assembly further includes a diverter defining a recess configured to receive a carrier, the diverter being rotatable between (i) a first position at which the recess is aligned with the carrier conveyor assembly and (ii) a second position at which the recess is aligned with the first position of the gripper.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
A method for reconstituting a reagent within a reagent pack including one or more wells and at least one of the wells containing a lyophilized reagent comprises the steps of receiving reagent packs into a reagent pack input carousel, transferring one reagent pack with a rotary distributor from the input carousel to a reagent pack storage carousel, retrieving the reagent pack from the storage carousel with the rotary distributor and placing the reagent pack into a reagent pack loading station, and dispensing a reconstitution reagent with an automated pipettor into the well containing the lyophilized reagent to form a reconstituted reagent.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
A method and apparatus for freezing a liquid droplet includes dispensing, by a liquid dispenser, a droplet of liquid into a fluid chamber containing a freezing fluid. The droplet of liquid is allowed to dwell in the freezing fluid for at least a predetermined dwell time so that the droplet of liquid freezes to a frozen droplet. The method and apparatus further includes injecting, by a gas injector, a stream of gas transversely to a surface of the freezing fluid at about where the frozen droplet is located along the surface of the freezing fluid contained in the fluid chamber so that the frozen droplet sinks in the freezing fluid.
F25D 3/11 - Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air with conveyors carrying articles to be cooled through the cooling space
B01J 2/06 - Processes or devices for granulating materials, in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic by dividing the liquid material into drops, e.g. by spraying, and solidifying the drops in a liquid medium
15.
METHOD FOR READING MACHINE-READABLE MARKS ON RACKS AND RECEPTACLES
A method of reading machine-readable marks on a movable support and object of a sample instrument. The method includes capturing a first image of the moveable support as the moveable support moves from a first position to a second position using an image capture device; determining whether a first fiducial machine-readable mark on the moveable support is in the first image; determining, when the first fiducial machine-readable mark is in the first image, whether a first machine-readable mark on a first object coupled to the moveable support is in the first image at a predetermined position relative to the first fiducial machine-readable mark; and associating information decoded from the first machine-readable mark on the first object with a first location on the moveable support associated with the first fiducial machine-readable mark.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G06K 7/14 - Methods or arrangements for sensing record carriers by corpuscular radiation using light without selection of wavelength, e.g. sensing reflected white light
G06K 19/06 - Record carriers for use with machines and with at least a part designed to carry digital markings characterised by the kind of the digital marking, e.g. shape, nature, code
A method of performing a lab developed test for detecting a nucleic acid analyte on an automated analyzer. The method includes a first step of using a computer to select, define or modify one or more user-defined parameters of a protocol for performing the lab developed test on the analyzer, where each user-defined parameter of the protocol defines a step to be performed by the analyzer during the lab developed test. The method further includes a second step of performing the lab developed test with the protocol of the first step, where the analyzer stores one or more system-defined parameters for performing the lab developed test, the one or more system-defined parameters being installed on the analyzer prior to performing first step.
A receptacle delivery system that includes a rail extending between first and second locations of an instrument and a carriage configured to move on the rail between the first and second locations of the instrument. The carriage includes a bracket having opposed first and second sidewalls and a base extending between the first and second sidewalls, where the carriage is configured to support a receptacle. The carriage further includes a pair of opposed support pads, where the pair of support pads are configured to (a) move toward a receptacle supported by the carriage as the carriage moves from the first location toward the second location, and (b) move away from a receptacle supported by the carriage as the carriage moves from the second location toward the first location. The carriage also includes a pair of meshed cam gears rotatably coupled to the first sidewall, where each cam gear is coupled to a different one of the support pads.
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
An apparatus for performing nucleic acid amplification reactions includes a thermally-conductive receptacle holder that includes multiple receptacle wells. Each well can receive a receptacle and has a through-hole extending from an inner surface of the well to an outer surface of the holder. The apparatus includes multiple optical fibers, and each optical fiber has a first end in optical communication with an associated well and a second end in optical communication with an excitation signal source and/or an emission signal detector. The first end of each optical fiber is moveable with respect to the through-hole. A cover is movable between an open position and a closed position relative to the receptacle holder, and the first end of each optical fiber moves with respect to the through-hole of the associated well as the cover moves to the open or closed position or when the cover moves into or out of contact with any receptacles within the wells.
A method of reading machine-readable labels on sample receptacles. In the method, a sample rack is moved between a first position and a second position withing a housing, where the sample rack supports a plurality of sample receptacles, and each sample receptacle has a machine-readable label. An absolute position of the sample rack is measured as the sample rack moves between the first and second positions. An image of the machine-readable label associated with each sample receptacle is acquired as the sample rack moves between the first and second positions. Finally, the acquired image of each machine-readable label is decoded.
G06K 7/10 - Methods or arrangements for sensing record carriers by corpuscular radiation
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G06K 7/14 - Methods or arrangements for sensing record carriers by corpuscular radiation using light without selection of wavelength, e.g. sensing reflected white light
G06K 19/06 - Record carriers for use with machines and with at least a part designed to carry digital markings characterised by the kind of the digital marking, e.g. shape, nature, code
A container including a top wall and a fluid vessel depending from the top wall. The fluid vessel extends from a top wall aperture formed in the top wall to a bottom end of the fluid vessel. The container further includes gripping structures configured to enable an automated gripping mechanism to securely grip the container in a repeatable position and orientation with respect to the container-gripping components of the gripping mechanism.
An apparatus including a thermally-conductive receptacle holder having a row of receptacle wells. Each receptacle well has an inner surface defined to receive a receptacle, and each receptacle well has a through-hole extending between the inner and outer surfaces thereof. The apparatus further includes one or more thermal elements for altering a temperature(s) of the receptacle holder, where the one or more thermal element are in contact with a side surface of the receptacle holder. There are two or more thermistors disposed in contact with the receptacle holder and a channel disposed within the side surface and containing wires and/or electrical connections for the two or more thermistors. The apparatus additionally includes a plurality of optical fibers, each optical fiber providing optical communication between one of the receptacle wells and at least one of an excitation signal source and an emission signal detector, where a first end of each optical fiber is disposed outside, within, or extends through the through-hole of a corresponding one of the receptacle wells, and where a second end of each optical fiber is in optical communication with the excitation signal source and/or the emission signal detector.
Disclosed are nucleic acid oligonucleotides, including primers, probes, and target capture oligonucleotides, for detection of SARS-CoV-2, Influenza A, and Influenza B. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligonucleotides. Corresponding formulations, reaction mixtures, and kits and related methods for preparing aqueous reaction mixtures from dried formulations are described.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The approach employs a single fixed data point in combination with a single adjustment calibrator amplified on the instrument that is to be calibrated for approximating a complete calibration curve.
G16B 40/00 - ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
A method and apparatus for freezing a liquid droplet includes dispensing, by a liquid dispenser, a droplet of liquid into a fluid chamber containing a freezing liquid. The droplet of liquid is allowed to dwell in the freezing liquid for at least a predetermined dwell time so that the droplet of liquid freezes to a frozen droplet. The method and apparatus further includes injecting, by a gas injector, a stream of gas transversely to a surface of the freezing liquid at about where the frozen droplet is located along the surface of the freezing liquid contained in the fluid chamber so that the frozen droplet sinks in the freezing liquid.
F25D 3/11 - Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air with conveyors carrying articles to be cooled through the cooling space
B01J 2/06 - Processes or devices for granulating materials, in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic by dividing the liquid material into drops, e.g. by spraying, and solidifying the drops in a liquid medium
Systems and methods for transferring containers (100) include or employ a container loading interface (200), a container storage module (400), and a container distributor (300) configured to transfer containers from the container loading interface to the container storage module. The container loading interface includes movable support platform (202) that is movable between accessible and a non-accessible positions and a container loading transport (214). The container storage module includes a housing (402) and a container storage transport (418). The container distributor includes a container gripper (320) configured to grasp a container on the container loading transport and to transfer the container to the container storage transport in the container storage module.
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
B65G 29/00 - Rotary conveyors, e.g. rotating discs, arms, star-wheels or cones
B65G 47/84 - Star-shaped wheels or devices having endless travelling belts or chains, the wheels or devices being equipped with article-engaging elements
G01F 23/263 - Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields by measuring variations in capacitance of capacitors
A container gripping mechanism includes first and second linear rails disposed on opposed sides of a linear rail mount. A first linear rail guide coupled with the first rail is supported on a first gripper finger mount, and a second linear rail guide coupled with the second rail is supported on a second gripper finger mount. A first gripper finger is secured to the first gripper finger mount, and a second gripper finger is secured to the second gripper finger mount. A pinion gear driven by a drive motor engages racks attached to the first and second gripper finger mounts, such that rotation of the pinion in a first direction causes the first and second gripper fingers to move toward each other, and rotation of the pinion in a second direction causes the first and second gripper fingers to move away from each other.
A system includes a reagent pack input carousel, a carousel frame supporting the carousel, and a track on which the carousel frame is supported for movement of the carousel into and out of an instrument in drawer-like fashion. The carousel is rotatable about an axis of rotation and includes a plurality of reagent pack input stations arranged about the perimeter of the carousel. Each input station is adapted to carry a reagent pack, and the input stations are oriented at an angle with respect to a radial orientation relative to the axis of rotation. Each input station includes an alignment block located at a radially inner end of the station, and the block is received within a recess formed in an end of the reagent pack placed in the station to align and position of the reagent pack within the station.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
A method for fluid handling that includes reading information about a receptacle holder with an RFID reader and transmitting the information from an RFID transponder disposed on a first body of the receptacle holder that supports a fluid-containing receptacle. This information is used to align a robotic fluid transfer device with the first fluid-containing receptacle. Detecting a change in capacitance between an electrically conductive probe of the robotic fluid transfer device and an electrical ground or voltage source capacitively coupled to fluid contained in the first fluid-containing receptacle is an indication that the probe has contacted the fluid contained in the first fluid-containing receptacle. Once it is determined that the probe has contacted the fluid, the fluid transfer device aspirates a portion of the fluid contained in the first fluid-containing receptacle.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
G01F 23/26 - Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.
C12Q 1/6811 - Selection methods for production or design of target specific oligonucleotides or binding molecules
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
An apparatus for detecting an optical signal emission includes signal transmission fibers. Each fiber includes cores having the same spatial core arrangement at each end. The first ends are configured to be optically coupled to the signal emission sources. Each fiber is configured to transmit an optical signal between the first end and the second. The apparatus can also include a frame assembly securing the first ends of the fibers in a first spatial fiber arrangement corresponding to a spatial arrangement of the signal emission sources. The frame assembly can also secure the second ends of the fibers in a second spatial fiber arrangement different from the first spatial fiber arrangement. The apparatus can also include at least one signal detector configured to be optically coupled to the second ends of the fibers and configured to detect an optical signal emitted by each signal emission source.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
G02B 6/08 - Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings formed by bundles of fibres the relative position of the fibres being the same at both ends, e.g. for transporting images with fibre bundle in form of plate
31.
COMPOSITIONS AND METHODS FOR DETECTING HEV NUCLEIC ACID
Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
Disclosed are methods utilizing specific amplification of Candida sp. target nucleic acid for detecting the presence or absence of Candida sp. in a sample. Also disclosed are corresponding oligomers, including amplification oligomers, capture probes and detection probes, and combinations thereof, as well as corresponding reaction mixtures and kits.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
33.
AUTOMATED PROCESSING OF SAMPLES CARRIED IN SAMPLE CONTAINERS AND GROUPING SAMPLE CONTAINERS ACCORDING TO ASSAYS TO BE PERFORMED ON SAMPLES CONTAINED THEREIN
A system for processing a plurality of sample containers, each containing sample with at least one open assay associated therewith, includes two or more analyzers, each configured to perform at least one functional assay in receptacle apparatuses including a process number of two or more receptacle vessels, a conveyance for transporting containers to the analyzers, a buffer queue associated with each analyzer for holding containers to be processed by the associated analyzer, and a scanner associated with each analyzer. Each scanner scans machine-readable identification information associated with each container transported by the conveyance past the scanner and the open assay(s) for that container are identified based on the scanned information. The sample container is diverted into the associated buffer queue only if at least one open assay for that container matches at least one functional assay of the associated analyzer. The system attempts to accumulate at least the process number of sample containers with the same open assay so that the matching functional assay can be performed on the different samples in each of the process number of receptacle vessels of each receptacle apparatus.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
Disclosed are methods utilizing specific amplification of Candida sp. target nucleic acid for detecting the presence or absence of Candida sp. in a sample. Also disclosed are corresponding oligomers, including amplification oligomers, capture probes and detection probes, and combinations thereof, as well as corresponding reaction mixtures and kits.
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Automated in vitro specimen analyzer, handling and/or processing instruments for laboratory use Automated in vitro specimen analyzer, handling and/or processing instruments for medical use
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Automated in vitro specimen analyzer, handling and/or processing instruments for laboratory use Automated in vitro specimen analyzer, handling and/or processing instruments for medical use
Provided are methods, compositions, and systems for detecting nucleic acids of macrolide-resistant Mycoplasma geni-talium using FRET probes for detecting SNPs at position 2058 and 2059.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
39.
JIG FOR POSITIONING AND SECURING A FIRST CONSOLE WITH RESPECT TO A SECOND CONSOLE
A jig assembly for connecting first and second consoles with predefined relative positioning includes first and second positioning pins attached or attachable to the first console and a positioning connection bracket attached or attachable to the second console and including a longitudinal span and first and second transverse legs including first and second pin recesses at respective free ends thereof. The first and second pin recesses receive the first and second positioning pins, respectively, when the first and second consoles are moved laterally toward each other. The first and second transverse legs may be attached to the first console with a first and second leg attachment brackets, respectively. The first and second positing pins and the first and second leg attachment brackets may be fixed with respect to the first console by attachment to a mounting rail that is fixed to the first console.
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
40.
DETECTING BABESIA SPECIES NUCLEIC ACID IN A SAMPLE
There is described herein a method for specifically detecting Babesia species nucleic acid in a sample, which in one aspect comprises: (1) contacting a sample, said sample suspected of containing Babesia species nucleic acid, with at least two oligomers for amplifying a target region of a Babesia species target nucleic acid, wherein the at least two amplification oligomers comprise: (a) a first amplification oligomer comprising a first target-hybridizing sequence (i) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:66 and comprises SEQ ID NO:56 or 57; or (ii) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:96 and comprises SEQ ID NO:101; or (iii) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:97 and comprises SEQ ID NO:101; (iv) comprises or consists of SEQ ID NO:8; (v) comprises or consists of SEQ ID NO:83 and (b) a second amplification oligomer comprising a second target-hybridizing sequence that is from about 15 to about 33 contiguous nucleotides in length, and (i) is contained in SEQ ID NO:68 and comprises SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, or SEQ ID NO:85; or (ii) is contained in SEQ ID NO:67 and comprises SEQ ID NO:45 or SEQ ID NO:52; or (iii) is contained in SEQ ID NO:70 and comprises SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, or SEQ ID NO:51; (2) performing an in vitro nucleic acid amplification reaction, wherein any Babesia target nucleic acid present in said sample is used as a template for generating an amplification product; and (3) detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Babesia species target nucleic acid in said sample.
C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Disclosed are formulations, including both liquid and lyophilized formulations, comprising a far-red dye probe and a non-linear surfactant or foamban. Also disclosed are related methods for preparing a lyophilized far-red dye probe formulation as well as related kits and diagnostic products.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12Q 1/6865 - Promoter-based amplification, e.g. nucleic acid sequence-based amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
C09B 23/08 - Methine or polymethine dyes, e.g. cyanine dyes characterised by the methine chain containing an odd number of CH groups more than three CH groups, e.g. polycarbocyanines
C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
42.
Automated sample handing instrumentation, systems, processes, and methods
A sample processing station includes two or more container holders on a platform that is rotatable about a central axis of rotation. Each holder is configured to rotate about a secondary axis of rotation. The station includes a capping/decapping mechanism to cap or decap a container held in one of the container holders and an elevator with a chuck guide that contact the container holder as the chuck is lowered by the elevator to positon the chuck with respect to the cap of the container held in the holder and to hold jaws of the container holder in a closed position. In embodiment, the chuck guide includes a yoke with opposed arms and spindles located near distal ends of the arms that engage beveled shoulders of the container holder.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01F 23/263 - Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields by measuring variations in capacitance of capacitors
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
B67B 7/00 - Hand- or power-operated devices for opening closed containers
C40B 60/12 - Apparatus specially adapted for use in combinatorial chemistry or with libraries for screening libraries
The disclosed disclosure is related to methods, compositions, and kits for targeting Adenovirus, Metapneumovirus, and/or Rhinovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
44.
COMPOSITIONS AND METHODS TO DETECT ADENOVIRUS NUCLEIC ACIDS AND METAPNEUMOVIRUS AND/OR RHINOVIRUS NUCLEIC ACIDS
The disclosed disclosure is related to methods, compositions, and kits for targeting Adenovirus, Metapneumovirus, and/or Rhinovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.
C12Q 1/6811 - Selection methods for production or design of target specific oligonucleotides or binding molecules
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
Automated systems and methods determine a level of fluid relative to a rim of a sample receptacle defining an open top of the sample receptacle. The systems and methods utilize a distance sensor to measure the distance between the rim of the sample receptacle and the surface of a fluid sample contained in the sample receptacle, where at least one of the sensor and the sample receptacle is moved relative to the other to enable the sensor to obtain a sequence of discrete measurements of distances between the sensor and the rim of the sample receptacle and between the sensor and the surface of the fluid sample. A controller processes an output signal from the sensor to determine a level of the fluid relative to the rim of the sample receptacle. The derivative of the sequence of discrete measurements may be used to identify the rim and the fluid surface in the output signal.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
A gripper apparatus is configured to grasp a closed receptacle having a closure affixed to an open top end of a receptacle. Opposed jaw members capable of lateral movement between an open position, a first closed position, and a second closed position are configured to grasp the closed receptacle when the closed receptacle is situated between the jaw members at the first closed position and to release the closed receptacle at the open position. The gripper apparatus further includes a plurality of fingers configured to grasp a sidewall of the closure beneath a top surface of the closure when the closure is situated between the plurality of fingers and beneath a base of each of the jaw members as the jaw members move laterally toward each other from the open position to the second closed position.
An apparatus for transporting groups of consumables between vertically spaced holding shelves that includes a support chassis laterally spaced from the holding shelves, a transport elevator coupled to the support chassis, a lift platform, and a scissors actuator connecting the lift platform to the support chassis and configured to laterally translate the lift platform between a first position laterally aligned with the support chassis and a second position laterally aligned with one of the holding shelves.
Disclosed are nucleic acid oligomers, including amplification oligomers, detection probes, and capture probes, for detection of SARS-CoV-2 nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits and related methods for preparing aqueous reaction mixtures from dried formulations.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
A multiplexed nucleic acid amplification and detection system useful for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms (i.e., "SNPs") in a temperature-dependent fashion using only a single fluorescence detection channel of a nucleic acid analyzer. The technique can be carried out using standard PCR instrumentation equipped for fluorescence detection or monitoring.
i.e.i.e., "SNPs") in a temperature-dependent fashion using only a single fluorescence detection channel of a nucleic acid analyzer. The technique can be carried out using standard PCR instrumentation equipped for fluorescence detection or monitoring.
A method for performing a magnetic separation procedure that includes transporting a receptacle containing a fluid medium to a first location of a system, where the fluid medium contains both a sample material and a suspension of magnetically-responsive solid supports. At the first location, the fluid medium is exposed to a first magnetic field for a first dwell period, thereby isolating the solid supports within the receptacle, where no portion of the fluid medium is removed from the receptacle at the first location. The receptacle is then transported from the first location to a second location of the system, where the fluid medium is exposed to a second magnetic field for a second dwell period. Following the second dwell period, at least a portion of the fluid medium is removed from the receptacle. A suspension fluid is then dispensed into the receptacle, and the contents of the receptacle are agitated to suspend the solid supports within the suspension fluid.
The disclosed disclosure is related to methods, compositions, and kits for targeting Adenovirus, Metapneumovirus, and/or Rhinovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
57.
COMPOSITIONS AND METHODS FOR DETECTING CORONAVIRUS NUCLEIC ACID
Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for amplification and/or detection of human coronavirus OC43,HKU1, NL63, and/or 229E nucleic acid. Also disclosed are methods of nucleic acid amplification and/or detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
58.
QUANTIFICATION OF POLYNUCLEOTIDE ANALYTES FROM DRIED SAMPLES
Presented are methods, systems, and software products useful for determining the concentration of an analyte in a fluid specimen used to produce a dried sample, where the dried sample serves as a source of the analyte in a detection and quantification procedure. Particularly illustrated is the use of dried blood spots for quantifying a polynucleotide analyte.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
59.
COMPOSITIONS AND METHODS FOR DETECTING GASTROINTESTINAL PATHOGENS
Compositions, methods, kits, and uses are provided for detecting the presence of Salmonella, Shigella, Campylobacter, and Shigatoxigenic Eschelichia coli (STEC) in samples. In some embodiments, the compositions, methods, kits, and uses comprise one or more oligonucleotides or the use of one or more oligonucleotides. In some embodiments, the method is carried out as a multiplex assay with separate primers/probes for C.jejuni and C.coli. Some of the oligonucleotides contain inosine and/or contain 5-methylcytosine substituted nucleotides. Described is the use of cyclodextrin and/or Polysorbate 20 in the amplification reactions.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
60.
AUTOMATED SYSTEMS AND METHODS FOR LOADING SOLID REAGENT PELLETS INTO WELLS OF A CARTRIDGE
Systems and methods for loading reagent pellets into wells of a cartridge include a pellet transfer head including vacuum nozzles corresponding to the number and arrangement of the wells of the cartridge, a pellet reservoir holding reagent pellets, a vision sensor system for performing various system vision checks, and vertical and horizontal carriage assemblies to effect relative movement between the transfer head, pellet reservoir, cartridge, and vision sensor system. The pellet transfer head may include downwardly-facing pressure ports to supply gas flow into the pellet supply reservoir to fluidize the supply of pellets and a static eliminator fan to eliminate or reduce static buildup among pellets in the reservoir. A pellet is drawn from the reservoir to each vacuum nozzle, and the vision sensor system confirms that a single, unbroken and undeformed pellet is positioned at each nozzle and then confirms transfer of a single pellet to each well.
Salmonella, Shigella, Campylobacter,Eschelichia coliEschelichia coli (STEC) in samples. In some embodiments, the compositions, methods, kits, and uses comprise one or more oligonucleotides or the use of one or more oligonucleotides. In some embodiments, the method is carried out as a multiplex assay with separate primers/probes for C.jejuni and C.coli. Some of the oligonucleotides contain inosine and/or contain 5-methylcytosine substituted nucleotides. Described is the use of cyclodextrin and/or Polysorbate 20 in the amplification reactions.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Sample receptacles are automatically transferred from first and second storage areas of a storage module to a conveyor without passing over any other sample receptacle stored in the storage module. Receptacle holding positions in the first and second storage areas are arranged in rows and columns, and sample containers are transferred row-by-row or column-by-column, starting with sample container held closest to a first side of the first or second storage area. Receptacles transferred from the first storage area are transferred across the first side to the conveyor. Receptacles transferred from the second storage area are transferred across the first side into a first receptacle transport path between the first and second storage areas, and then to a second receptacle transport path across the first storage area, and then to the conveyor. To avoid passing a receptacle over another receptacle, receptacles are first removed from intervening holding positions between a respective holding position from which the receptacle is being transferred and the first side of the first or second storage area.
B65G 47/90 - Devices for picking-up and depositing articles or materials
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
64.
ENZYME FORMULATIONS AND REACTION MIXTURES FOR NUCLEIC ACID AMPLIFICATION
Disclosed are formulations and reaction mixtures comprising at least one polymerase, a-cyclodextrin, and polysorbate 20. Also disclosed are related methods for preparing the disclosed formulations and reaction mixtures, as well as related kits and methods for nucleic acid amplification. The formulations and reaction mixtures are useful, for example, in mitigating the inhibitory effect of sample extraction detergents on nucleic acid amplification reactions.
Disclosed are formulations and reaction mixtures comprising at least one polymerase, α-cyclodextrin, and polysorbate 20. Also disclosed are related methods for preparing the disclosed formulations and reaction mixtures, as well as related kits and methods for nucleic acid amplification. The formulations and reaction mixtures are useful, for example, in mitigating the inhibitory effect of sample extraction detergents on nucleic acid amplification reactions.
A method for sharing system information data in a network of two or more diagnostic instruments for performing assays is provided. The system information data includes operating information for the diagnostic instruments in the network. The method includes: sending a system information metadata packet to at least a first diagnostic instrument in the network, wherein the system information metadata packet, is associated with system information data on a second diagnostic instrument in the network and comprises one or more attributes of a system information data packet containing the associated system information data; receiving a request from the first diagnostic instrument to send the system information data packet, and in response to the request from the first diagnostic instrument to send the system information data packet, sending the system information data packet containing the associated system information data to the first diagnostic instrument.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
67.
Compositions and Methods for Capturing Target Nucleic Acids
Capture mixtures and activated capture mixtures are provided that are useful for nucleic acid separation and purification are provided. The mixtures comprise lithium lauryl sulfate, lithium hydroxide, a zwitterionic sulfonic acid buffering agent, and optionally, proteinase K, capture probes comprising a first specific binding partner (SBP), and a second specific binding partner immobilized to a solid support. Related combinations, methods, uses, and kits, are also provided.
Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detection of Group B Streptococcus (GBS; Streptococcus agalactiae) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
This disclosure provides oligomers, compositions, and kits for detecting and quantifying Hepatitis C virus (HCV), including different genotypes and variants thereof, and related methods and uses. In some embodiments, oligomers target the 5′ untranslated region of HCV and are configured to provide substantially equivalent quantification of different genotypes and variants of HCV.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
The disclosed disclosure is related to methods, compositions, and kits for targeting Adenovirus, Metapneumovirus, and/or Rhinovinis nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
An assembly includes a bracket, a magnetic elution slot, and a reagent pack loading station. The elution slot receives a multiple receptacle device having multiple receptacles interconnected by a connecting rib defining shoulders along either side of the receptacle device. The elution slot includes opposed walls attached to the bracket below a top surface of the bracket with the opposed walls disposed on either side of a slot formed in the bracket. The downwardly facing shoulders of the receptacle device are supported on the top surface of the bracket on opposed sides of the slot, and one or more magnets are attached to or embedded in one or both walls. The reagent pack loading station holds a multi-well reagent pack such that the wells of the reagent pack are accessible by a pipettor, and the reagent pack holding station includes spaced apart hold down features extending above the top surface of the bracket.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Nucleic acid oligomers, including amplification oligomers and detection probes, for detection of human adenovirus nucleic acid by binding to the penton gene. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits and related methods for preparing aqueous reaction mixtures from dried formulations. Oligomers and methods of detecting one or more groups selected from group A, B, C, D, E and F are also disclosed.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
A method for measuring optical signal detector performance that includes directing light emitted from an optical signal detector onto a first non-fluorescent surface portion in a first detection zone of the optical signal detector. A first characteristic of light detected by a first sensor of the first optical signal detector is measured while the first non-fluorescent surface portion is in the first detection zone of the optical signal detector. Light emitted from the optical signal detector is directed into a first void in the first detection zone of the optical signal detector. A second characteristic of light detected by the first sensor of the optical signal detector is measured while the first void is in the first detection zone of the optical signal detector. And an operational performance status of the optical signal detector is determined based on at least one of the first characteristic and the second characteristic.
G01N 21/27 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
G01N 21/75 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
75.
System and method for receiving and storing reagent packs in an instrument
A system includes a reagent pack input carousel, a carousel frame supporting the carousel, and a track on which the carousel frame is supported for movement of the carousel into and out of an instrument in drawer-like fashion. The carousel is rotatable about an axis of rotation and includes a plurality of reagent pack input stations arranged about the perimeter of the carousel. Each input station is adapted to carry a reagent pack, and the input stations are oriented at an angle with respect to a radial orientation relative to the axis of rotation. Each input station includes an alignment block located at a radially inner end of the station, and the block is received within a recess formed in an end of the reagent pack placed in the station to align and position of the reagent pack within the station.
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
A reagent pack changer includes a reagent pack input device, a reagent pack storage compartment, a reagent pack storage carousel disposed within the storage compartment, and a rotary distributor. The input device includes a reagent pack input carousel rotatable about an axis of rotation, with reagent pack stations for holding reagent packs disposed around the axis of rotation of the carousel. The reagent pack storage carousel is rotatable about an axis of rotation with reagent pack stations for holding reagent packs disposed around the axis of rotation. The rotary distributor is configured to move a reagent pack between the reagent pack input carousel and the reagent pack storage carousel.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
A receptacle distribution system includes a rotary distributor and a receptacle handoff device. The rotary distributor includes a motorized turntable with an upright wall, a distributor head for holding a receptacle, a hook configured to engage a receptacle for moving the receptacle into or out of the distributor head, and an a system for powered elevation of the distributor head. The receptacle handoff device is configured to transfer a receptacle from a first receptacle distributor to the rotary distributor and includes a receptacle yoke configured to hold a receptacle placed in the receptacle yoke with the first receptacle distributor, the receptacle yoke being rotatable between a first position to receive the receptacle and a second position to present the receptacle to the rotary distributor to enable the hook of the rotary distributor to pull the receptacle from the receptacle yoke and into the distributor head.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Nucleic acid oligomers, including amplification oligomers and detection probes, for detection of human adenovirus nucleic acid by binding to the penton gene. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding formulations, reaction mixtures, and kits and related methods for preparing aqueous reaction mixtures from dried formulations. Nucleic acid oligomers and methods for nucleic acid amplification and detection of one or more adenovirus subgroups A, B, C, D, E and F are also disclosed.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
A receptacle delivery system includes a carriage configured to move from a first to a second location of an instrument. The carriage may be configured to removably support a receptacle and may include a receptacle clamping mechanism. The receptacle mechanism may be configured to apply a clamping force to the receptacle as the carriage moves from the first to the second location and release the clamping force as the carriage moves from the second to the first location.
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
Hybridization probe reagents that specifically detect nucleic acids of C. trachomatis, including wildtype and/or variant sequences identified as FI-nvCT C1515T (SEQ ID NO: 17), JP-nvCT C1522T (SEQ ID NO: 12), US-nvCT G1526A (SEQ ID NO:22), and NO-nvCT G1523A (SEQ ID NO:27). Certain probes include nucleotide analogs to enhance desirable binding properties.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
A nucleic acid diagnostic system includes a unit-dose reagent compartment storing at least one unit-dose pack, one or more unit-dose pack loading stations for holding a unit-dose pack at a location that permits a substance transfer device to dispense a reconstitution reagent into each well of the pack, a unit-dose pack distributor configured to transfer a unit-dose pack from the unit-dose reagent compartment to one of the unit-dose pack loading stations, and an electrostatic generator disposed within the housing of the unit-dose reagent compartment and/or adjacent the one or more unit-dose pack loading stations. The electrostatic generator imparts an electrostatic charge to each of the wells of the unit-dose pack and/or to a lyophilized reagent pellet within each well so that the electrostatic charge positions and holds each lyophilized reagent pellet at a bottom of each respective well of the unit-dose pack.
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
A cap and a processing vial configured for interlocking engagement. The processing vial includes a locking collar surrounding an open upper end of the processing vial with lateral through holes formed through the locking collar. The vial also includes a latch hook located above each through hole. The cap includes a latch collar extending about a lower portion of the cap, where the latch collar is configured to snap in beneath the latch hooks of the processing vial when the lower portion of the cap is inserted into the open upper end of the processing vial to lock the cap to the processing vial.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
This disclosure provides oligomers, combinations of oligomers, compositions, kits, uses, and methods for detecting a C1orf43 nucleic acid, such as C1orf43 mRNA, such as human C1orf43 mRNA, in a sample.
Methods for use in multiplex amplification and or detection of Trichomonas vaginalis. The multiphase amplification provides fast, quantitative, sensitive detection with lower variability at low analyte concentrations. Described are detection probes, capture probes, amplification oligonucleotides, nucleic acid compositions, probe mixes, methods, and kits useful for amplifying and determining the presence of Trichomonas vaginalis in a test sample.
C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
C12Q 1/6865 - Promoter-based amplification, e.g. nucleic acid sequence-based amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
85.
COMPOSITIONS AND METHODS FOR BIOLOGICAL SAMPLE PROCESSING
Methods, apparatuses, and systems are provided for processing a biological sample. Exemplary methods comprise transferring a swab associated with an aliquot of the biological sample into a sample transport media. The methods can provide efficient transfer of a target material such as cells or nucleic acid into the sample transport media for extraction, amplification, and detection of the target material.
Methods, apparatuses, and systems are provided for processing a biological sample. Exemplary methods comprise transferring a swab associated with an aliquot of the biological sample into a sample transport media. The methods can provide efficient transfer of a target material such as cells or nucleic acid into the sample transport media for extraction, amplification, and detection of the target material.
Methods, apparatuses, and systems are provided for processing a biological sample. Exemplary methods comprise transferring a swab associated with an aliquot of the biological sample into a sample transport media. The methods can provide efficient transfer of a target material such as cells or nucleic acid into the sample transport media for extraction, amplification, and detection of the target material.
A first time/temperature data set includes temperatures and time stamps recorded at time intervals during a first thermal cycling process and a time stamp for each time interval, and a second time/temperature data set includes temperatures and time stamps recorded at time intervals during a second thermal cycling process. Signal emissions at different signal detecting positions are sequentially measured at different time intervals to generate first and second time/signal emission data sets for receptacles subject to the first and second thermal cycling processes, respectively. Signal emissions of receptacles subject to the first thermal cycling process are synchronized with specific temperatures of the first thermal cycling process by comparing time stamps of the first time/signal emission data set for each receptacle with the time stamps of the first time/temperature data set that correspond with the specific temperature. Signal emissions of receptacles subject to the second thermal cycling process are synchronized with specific temperatures of the second thermal cycling process by comparing time stamps of the second time/signal emission data set for each receptacle with the time stamps of the second time/temperature data set that correspond with the specific temperature.
G02B 6/06 - Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings formed by bundles of fibres the relative position of the fibres being the same at both ends, e.g. for transporting images
G02B 6/08 - Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings formed by bundles of fibres the relative position of the fibres being the same at both ends, e.g. for transporting images with fibre bundle in form of plate
G01N 21/25 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
There is disclosed a composition of an aqueous solution comprising, consisting or consisting essentially of a flap endonuclease, a bulking agent and an organic buffer, wherein the aqueous solution has an inorganic salt concentration of 5 mM or less and wherein the composition is substantially free of glycerol.
Disclosed are nucleic acid oligomers, including amplification oligomers, detection probes, and capture probes, for detection of Plasmodium species nucleic acid in a sample. Also disclosed are methods of specific nucleic acid amplification and detection, including amplification and detection of target nucleic acid in real time, using the disclosed oligomers, as well as corresponding reaction
C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
A cap having a lower portion, an upper portion with an opening formed therein, and a plurality of longitudinally oriented ribs disposed on an inner surface of the cap. The inner surface of the cap is defined by the opening formed in the upper portion of the cap, and each rib is associated with a concave recess disposed directly opposite the rib on an outer surface of the upper portion of the cap. The recess extends along at least part of the length of the rib.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B65B 69/00 - Unpacking of articles or materials, not otherwise provided for
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
B65D 39/00 - Closures arranged within necks or pouring openings or in discharge apertures, e.g. stoppers
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
A method for detecting the presence of a pathogen in a whole blood sample aliquot, includes providing a sample aliquot from each of a number of whole blood samples, thereby providing a plurality of whole blood sample aliquots, separately contacting each of the whole blood sample aliquots of the plurality of whole blood sample aliquots with a lysis reagent, whereby at least a portion of the blood cells in each of the whole blood sample aliquots lyse, pooling the lysed whole blood sample aliquots to form a pooled lysate, and testing the pooled lysate for presence of a pathogen. Identification of the presence of the pathogen in the pooled lysate indicates the presence of the pathogen in at least one of the whole blood sample aliquots.
G01N 1/38 - Diluting, dispersing or mixing samples
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
A system for managing liquid waste includes a first liquid container that receives waste liquid, a liquid transfer pump fluidly connected to the first liquid container; and a second liquid container fluidly connectable to the liquid transfer pump. The liquid transfer pump is can be selectively activated to transfer liquid waste from the first liquid container to the second liquid container when the second liquid container is fluidly connected to the liquid transfer pump. A method for managing liquid waste includes the steps of receiving waste liquid into a first liquid container, monitoring the amount of liquid in the first container, connecting a second liquid container to a liquid transfer pump that is connected to the first liquid container, after the amount of liquid received into the first liquid container reaches a predefined level, transferring liquid from the first liquid container into the second liquid container with the liquid transfer pump, and removing liquid transferred to the second liquid container.
F04B 49/025 - Stopping, starting, unloading or idling control by means of floats
F04B 37/14 - Pumps specially adapted for elastic fluids and having pertinent characteristics not provided for in, or of interest apart from, groups for special use to obtain high vacuum
F04B 45/02 - Pumps or pumping installations having flexible working members and specially adapted for elastic fluids having bellows
Method and associated system for reading machine-readable labels on a plurality of sample receptacles held by a sample rack. In the method, a machine-readable label associated each of the plurality of sample receptacles is read with a first label reader when the rack is at a first location. The sample rack is then moved from the first location to a second location, where a rack identifier on the sample rack is sensed with a sensor separate from the first label reader. Finally, the rack identifier is associated with the machine-readable labels of the plurality of sample receptacles.
G06K 7/10 - Methods or arrangements for sensing record carriers by corpuscular radiation
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G06K 7/14 - Methods or arrangements for sensing record carriers by corpuscular radiation using light without selection of wavelength, e.g. sensing reflected white light
G06K 19/06 - Record carriers for use with machines and with at least a part designed to carry digital markings characterised by the kind of the digital marking, e.g. shape, nature, code
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
After disposable pipette tips are used by an automated pipettor, they are released by the pipettor and fall into a waste container. When the waste container is removed to be emptied, the pipette tips are temporarily sequestered in a pipette tip holding station so that the automated pipettor may operate uninterrupted. After the waste container is replaced, the sequestered pipette tips are released by the holding station into the waste container.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
97.
Apparatus for maintaining a controlled environment
A lyophilization nest and method of using the same is described herein. In various embodiments, the lyophilization nest includes a base and first and second covers and is configured to support one or more receptacles each supporting one or more substances within interior spaces of the lyophilization nest. The interior spaces may be in fluid communication with the exterior of the lyophilization nest through one or more vent holes extending through the first and second covers. Each of the one or more vent holes have a corresponding sealing element configured to selectively form an air-tight seal within the vent holes, such that a controlled environment may be maintained within the interior spaces when the ambient conditions surrounding the lyophilization nest are not lyophilization conditions. The one or more sealing elements may be operable while the lyophilization nest is positioned within a sealed lyophilizer by depressing the sealing elements into corresponding vent holes to form the air-tight seal.
F26B 5/06 - Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum the process involving freezing
A method for performing a nucleic acid amplification assay employs a thermally-conductive receptacle holder with one or more receptacle wells, each conforming to an outer surface of a lower portion of a vial. A through-hole extends from an inner surface of each well to an outer surface of the holder. A thermal element is positioned proximal to the holder for altering a temperature of the holder. A signal detection module is configured to generate an excitation signal directed through the through-hole of the well and detect an optical emission from a fluid contained in the vial supported by the well. At least one well supports a capped vial containing a reagent for a nucleic acid amplification assay and including an opaque cap sealing an open end of the vial. The lower portion of the vial is contained within a well, and the cap is situated above a top surface of the holder.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.
C12Q 1/6811 - Selection methods for production or design of target specific oligonucleotides or binding molecules
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
