Film seal assemblies that seal one or more reagents within a sample cartridge, which allow for single lid cartridge designs. Such film seal assemblies can include one or more layers, a bottom-most layer that permanently seals with a sample cartridge having multiple chambers for one or more reagents, and top-most layer that releasably seals vent opening(s) in the film seal assembly and is removable by the end user. This configuration allows use of a single lid, which can be integrally formed with cartridge body or a separate component attached atop the cartridge body. The film seal assembly can include peelable liner(s) or can releasably attached to an underside of the single lid. Such film assemblies can include multiple layers that include a polypropylene layer for permanently sealing to a rigid propylene cartridge body and polyester top layers for releasably and hermetically sealing with a liner or the lid.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B65D 17/50 - Non-integral frangible members applied to, or inserted in, preformed openings, e.g. tearable strips or plastic plugs
B65D 51/16 - Closures not otherwise provided for with means for venting air or gas
B65D 51/22 - Caps, lids, or covers co-operating with an inner closure arranged to be opened by piercing, cutting, or tearing having means for piercing, cutting, or tearing the inner closure
B65D 53/08 - Flexible adhesive strips adapted to seal filling or discharging apertures
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
2.
EXPONENTIAL BASE-GREATER-THAN-BASE 2 NUCLEIC ACID AMPLIFICATION USING HIGH- AND LOW-Tm PRIMERS
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. The method employs pairs of primers that differ significantly in Tm and a novel temperature/time course characterized by a temperature pulse during denaturation that enables a high-Tm primer (but not a low-Tm primer) to anneal and prime the synthesis of an additional nucleic acid strand beyond the two strands synthesized in a cycle of classical PCR. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR.
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
Methods and systems for detecting defect in sample cartridges in real-time during manufacturing. Such systems utilize one or more external sensors that detect characteristics or parameters of the sample cartridge and/or the manufacturing process from one or more data sets. The external sensor(s) include any of: an RGB camera, IR camera, high-resolution optical camera, and ultrasonic microphone or combination thereof. An automated system obtains data sets from external sensor(s) and compares the data sets to a baseline of the sample cartridge and/or manufacturing process such that defects can be determined based on a variance from the baseline. Such methods can utilize feature extraction and spectrum analysis to identify features or characteristics for comparison with the baseline. A machine learning model can be used to determine an algorithm based on data sets of acceptable sample cartridges and data sets from the external sensor(s) that are associated with the cartridge defect.
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control (DNC), flexible manufacturing systems (FMS), integrated manufacturing systems (IMS), computer integrated manufacturing (CIM)
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B29C 65/08 - Joining of preformed parts; Apparatus therefor by heating, with or without pressure using ultrasonic vibrations
Methods and system for identifying faulty sample cartridges in real-time during manufacturing are provided herein. Such systems use monitored operational parameters associated with operation of manufacturing equipment that manufactures the sample cartridges. One or more data sets of operational parameters are obtained from existing equipment controls and/or additional sensors and compared to corresponding operational parameters associated with acceptable cartridges. A faulty detection unit can be configured to compare the operational parameters and can optionally include algorithms and/or models, such as a machine learning model, by which faulty cartridges can be identified. The comparison can include determining whether the monitored operational parameters are within a pre-defined range of acceptable values and/or deviate from a characteristic profile of the operational parameter in a manner indicative of faulty cartridges. The faulty cartridge detection can be integrated within automation controls so that faulty cartridges can be detected in real-time and automatically discarded during manufacturing.
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control (DNC), flexible manufacturing systems (FMS), integrated manufacturing systems (IMS), computer integrated manufacturing (CIM)
6.
TRANSFER LEARNING METHODS AND MODELS FACILITATING DEFECT DETECTION
Methods and systems for training a model for automated defect detection of a product during manufacturing are provided herein. Such methods utilize a combination of supervised transfer learning through auxiliary tasks and a combination of supervised and unsupervised learning. The methods can utilize supervised transfer learning with expert labels on a generalized auxiliary task, such as product classification, which is transferred to more specific auxiliary tasks, such as identification of specific product features and/or anomaly detection, where additional expert labels are then applied to the anomalies, and another iteration of supervised learning further improves the model. The anomalies can correspond to features associated with defects, which can be induced experimentally to improve efficiency of the training procedure. The product can be a sample cartridge such that the model allows detection of faulty cartridges based on sample cartridge and/or manufacturing process data.
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having a well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.
Automated oligonucleotide synthesis-compatible sulforhodamine dye phosphoramidite compounds and labeled polynucleotides incorporating these dyes are provided.
Compositions and methods for isolating and detecting nucleic acid in a biological sample are provided. The compositions and methods utilize a modified solid support comprising an amine or amide group.
Systems for processing a fluid sample to facilitate analysis with a semiconductor detection chip are provided herein. Such systems can include a sample processing cartridge coupleable with a chip carrier device configured for transport of the processed fluid sample from the sample cartridge. The chip carrier device can include one or more fluid channels extending between fluid-tight couplings attachable to transfer ports of the sample processing cartridge. The chip carrier device can include multiple portions or adapters, including a fluid sample portion, a flowcell portion and a chip carrier. Also provided are methods of preparing and transporting a fluid sample from a sample cartridge into a chip carrier device for analysis with a semiconductor detection chip carried within the chip carrier device.
Test cartridges for performing multi-target assay panels, in particular multiplex vaginal panels (MVP) and methods of use and manufacture. Such cartridges can include a cartridge body having multiple chambers and rotatable valve assembly to facilitate controlled fluid flow, and various reagents and buffers to chemically lyse nucleic acids from multiple targets in MVP. Specialized valve assemblies have been utilized to resist alkaline lysing buffers required to chemically lyse multiple targets, including various protozoa, yeasts and bacteria, including Atopobium spp. (Atopobium vaginae, Atopobium novel species CCUG 55226); Bacterial Vaginosis-Associated Bacterium 2 (BVAB2); and Megasphaera-1. Methods include detecting levels of each respective target in a single sample from a single multi-target assay cartridge and identifying the subject as having bacterial vaginosis, vulvovaginal candidiasis (or the presence of Candida spp.), or trichomoniasis (or presence of Trichomonas vaginalis) based on detected levels of each of: Atopobium spp., BVAB2, Megasphaera-1, Candida spp., and Trichomonas vaginalis.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
12.
ENCODERLESS MOTOR WITH IMPROVED GRANULARITY AND METHODS OF USE
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor relative the substrate to be determined from linear portions of the sinusoidal signal without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02P 6/16 - Circuit arrangements for detecting position
H02K 29/08 - Motors or generators having non-mechanical commutating devices, e.g. discharge tubes or semiconductor devices with position sensing devices using magnetic effect devices, e.g. Hall-plates or magneto-resistors
H02K 11/00 - Structural association of dynamo-electric machines with electric components or with devices for shielding, monitoring or protection
H02K 15/02 - Methods or apparatus specially adapted for manufacturing, assembling, maintaining or repairing of dynamo-electric machines of stator or rotor bodies
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
14.
NUCLEIC ACID EXTRACTION AND ISOLATION WITH HEAT LABILE SILANES AND CHEMICALLY MODIFIED SOLID SUPPORTS
Compositions and methods for isolating and detecting nucleic acid in a biological sample are provided. The compositions and methods utilize a modified solid support comprising an amine or amide group.
Diagnostic detection chip device designs that reduce cost of fabrication and assembly are described herein. Such chip device designs include features that facilitate use of the chip within a chip carrier device with integrated fluid flow control features and compatibility with conventional sample cartridges and sample processing systems. Associated methods of manufacture and assembly of the chip devices are also provided herein.
Improved sample cartridges, valve assemblies and methods of manufacture and assembly are provided herein. Such systems can include a sample processing cartridge having a unitary cartridge body with integral syringe tube and valve interface. Such systems can further includes valve assemblies with an overmolded gasket and gaskets with a protruding conical valve sealing surface. Various additional features can include thin film sealing for cartridge as well as valve assemblies for chemical lysis. Thin film sealing for cartridge lids can include various multi-layered designs to facilitate injection and sealing of reagents within the cartridge. Magnetic separation features are also included. Such features can be included in various design iterations as needed for compatibility with existing technologies and to accommodate needs for manufacturing workflows.
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. The method employs pairs of primers that differ significantly in Tm and a novel temperature/time course characterized by a temperature pulse during denaturation that enables a high-Tm primer (but not a low-Tm primer) to anneal and prime the synthesis of an additional nucleic acid strand beyond the two strands synthesized in a cycle of classical PCR. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR.
RFID-based operations management systems and methods are provided herein, particularly RFID based Kanban systems. Such system can realize increased efficiencies, improved visibility of inventory throughout the workflow and improved response to variations in demand. RFID based solutions can automatically transact consumption of Kanban inventory in real-time and trigger replenishment signals to warehouses automatically, resulting in increased Kanban inventory accuracy and lower manual efforts for replenishment and consumption posting. This approach can interface with existing operational management software (e.g. ERP systems, such as SAP) seamlessly integrating with product manufacturing workflows. Such systems are uniquely applicable to manufacture of biological testing kits, where demands fluctuate considerably in response to crises, disasters, epidemics and pandemics.
G06Q 10/087 - Inventory or stock management, e.g. order filling, procurement or balancing against orders
G06K 19/07 - Record carriers with conductive marks, printed circuits or semiconductor circuit elements, e.g. credit or identity cards with integrated circuit chips
A remote monitoring system for medical data collection can include a data-flagging process embeds authorization and settings information into a file containing the test results. Such data flagging can occur at a medical device or testing site, and may be based in policy settings received from a remote system. A file containing the test results can also include data category information that can be used to protect sensitive information by preventing such information from being communicated to the wrong server.
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
H04L 67/12 - Protocols specially adapted for proprietary or special-purpose networking environments, e.g. medical networks, sensor networks, networks in vehicles or remote metering networks
Improved sub-assemblies and methods of control for use in a diagnostic assay system adapted to receive an assay cartridge are provided herein. Such sub-assemblies include: a brushless DC motor, a door opening/closing mechanism and cartridge loading mechanism, a syringe and valve drive mechanism assembly, a sonication horn, a thermal control device and optical detection/excitation device. Such systems can further include a communications unit configured to wirelessly communicate with a mobile device of a user so as to receive a user input relating to functionality of the system with respect to an assay cartridge received therein and relaying a diagnostic result relating to the assay cartridge to the mobile device.
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
H02P 27/06 - Arrangements or methods for the control of AC motors characterised by the kind of supply voltage using variable-frequency supply voltage, e.g. inverter or converter supply voltage using dc to ac converters or inverters
H02P 6/182 - Circuit arrangements for detecting position without separate position detecting elements using back-emf in windings
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
21.
PSEUDO-COMPLEMENTARY BASES IN GENOTYPING AND NUCLEIC ACID SEQUENCING
Described herein are methods and compositions that make use of pseudo-complementary bases to reduce unwanted hybridization in assays to detect and/or quantify particular nucleotide sequences, as well as in nucleic acid sequencing protocols.
Compositions and methods for detecting Mycobacterium tuberculosis (MTB) infection in a patient suspected of being infected with MTB and for distinguishing active tuberculosis (ATB), incipient tuberculosis (ITB) or subclinical tuberculosis (STB) from latent tuberculosis and other pulmonary and infectious diseases are provided. The methods may also be used to monitor treatment responses of MTB infected patients. Changes in the expression level of genes are used to aid in the diagnosis, prognosis, and treatment of tuberculosis.
Compositions and methods for detecting mycobacterium tuberculosis (MTB) infection in a patient suspected of being infected with MTB and for distinguishing active tuberculosis (ATB), incipient tuberculosis (ITB) or subclinical tuberculosis (STB) from latent tuberculosis and other pulmonary and infectious diseases are provided. The methods may also be used to monitor treatment responses of MTB infected patients. Changes in the expression level of genes are used to aid in the diagnosis, prognosis, and treatment of tuberculosis.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
24.
EXPONENTIAL BASE-3 AND GREATER NUCLEIC ACID AMPLIFICATION WITH REDUCED AMPLIFICATION TIME
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.
Described herein is a comprehensive novel amplification system that can employ novel nucleic acid constructs as blocked primers, such constructs including a target-specific region, wherein the target-specific region comprises a target-specific cleavage domain, and a universal adapter sequence located 5' of the target-specific region, wherein the universal adapter sequence comprises a non-natural nucleotide base. Also described is a fusion protein between a polymerase and an endonuclease, which is useful in this system, as well as related methods and kits.
Systems and methods for monitoring, characterizing and controlling operation of LEDs are provided herein. Methods includes measuring a voltage across the LED, and correlating the voltage to a junction temperature of the LED. This correlation can be used to improve operation of the LED by increasing the signal to noise ratio of the LED signal, characterize the LED by comparing to an I-V curve, control LED operation to compensate for LED degradation and avoid crosstalk, and/or to generally improve performance and life expectancy of the LED. Improved performance of the LED can include stabilizing the photon output during performance of an assay to provide a desired dye reporter signal required for the assay and/or reducing an intra-shot during of the LED output during the assay. System and device with control units configured to perform these methods are also described herein.
Systems and methods for monitoring, characterizing and controlling operation of LEDs are provided herein. Methods include measuring a voltage (V) across a LED (1), and correlating the voltage (V) to a junction temperature of the LED (1). This correlation can be used to improve operation of the LED (1) by increasing the signal to noise ratio of the LED signal (5), characterize the LED (1) by comparing to an l-V curve, control LED operation to compensate for LED degradation and avoid crosstalk, and/or to generally improve performance and life expectancy of the LED (1). Improved performance of the LED (1) can include stabilizing the photon output (5) during performance of an assay to provide a desired dye reporter signal required for the assay and/or reducing an intra-shot during of the LED output (5) during the assay. System and device with control units (2) configured to perform these methods are also described herein.
Reaction vessels, cartridges, devices and methods for facilitating high-level multiplexing are described herein. Such reaction vessels can include a planar frame defining a fluidic path between a first planar substrate and a second planar substrate, a fluidic interface is located at one end of the planar frame with a pair of fluidic ports, a well chamber and a pre-amplification chamber. Devices for spotting reagents in wells of high-level multiplexing reaction vessels and improved reagent solutions are also described herein.
Sample cartridge, valve assembly and processing methods for providing mechanical lysis, chemical lysis or both for a given fluid sample are provided herein. Such systems can include a sample processing cartridge having a valve assembly configured for transport of the processing of fluid sample within the sample cartridge. The valve assembly can include a valve body and cap that secure a filter therebetween and facilitate inflow of mechanical or chemical lysing agents as needed for a fluid sample. Assay workflows for performing both mechanical and chemical lysis of a fluid sample within the same workflow of a single universal sample cartridge are also provided.
Reaction vessels, cartridges, devices and methods for facilitating high-level multiplexing are described herein. Such reaction vessels can include a planar frame defining a fluidic path between a first planar substrate and a second planar substrate, a fluidic interface is located at one end of the planar frame with a pair of fluidic ports, a well chamber and a pre-amplification chamber. Devices for spotting reagents in wells of high-level multiplexing reaction vessels and improved reagent solutions are also described herein.
Sample cartridge, valve assembly and processing methods for providing mechanical lysis, chemical lysis or both for a given fluid sample are provided herein. Such systems can include a sample processing cartridge having a valve assembly configured for transport of the processing of fluid sample within the sample cartridge. The valve assembly can include a valve body and cap that secure a filter therebetween and facilitate inflow of mechanical or chemical lysing agents as needed for a fluid sample. Assay workflows for performing both mechanical and chemical lysis of a fluid sample within the same workflow of a single universal sample cartridge are also provided.
Sample cartridge, valve assembly and processing methods for providing mechanical lysis, chemical lysis or both for a given fluid sample are provided herein. Such systems can include a sample processing cartridge having a valve assembly configured for transport of the processing of fluid sample within the sample cartridge. The valve assembly can include a valve body and cap that secure a filter therebetween and facilitate inflow of mechanical or chemical lysing agents as needed for a fluid sample. Assay workflows for performing both mechanical and chemical lysis of a fluid sample within the same workflow of a single universal sample cartridge are also provided.
The present disclosure relates to aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.
A lid apparatus for a multi-chambered container. The lid apparatus has a top-lid that is hingedly attached to a bottom-cap. The top-lid includes one or more openings for fluid filling multiple passages that extend from the bottom-cap. A lower bottom-cap includes welding features for welding to the multi-chambered container. The bottom-cap further includes one or more auxiliary ports for injecting a reagent when the lid apparatus is in a closed configuration sealingly attached to the multi-chambered sample container.
The present disclosure is directed to methods and kits for discriminating between a bacterial infection and viral infection in a human subject. More specifically, the methods can comprise detecting the expression level of a combination of ABL1, IRF9, ITGAM, LY6E, PSTPIP2 and RUNX1 in biological samples from the human subject and determining whether the human subject has a bacterial or viral infection based on those expression levels.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
38.
METHODS FOR DISCRIMINATING BACTERIAL AND VIRAL INFECTIONS IN A HUMAN SUBJECT
The present disclosure is directed to methods and kits for discriminating between a bacterial infection and viral infection in a human subject. More specifically, the methods can comprise detecting the expression level of a combination of ABL1, IRF9, ITGAM, LY6E, PSTPIP2 and RUNX1 in biological samples from the human subject and determining whether the human subject has a bacterial or viral infection based on those expression levels.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
Thermal control devices and methods to provide improved control, speed and efficiency in temperature cycling are provided herein. Such thermal control device and methods can include one or more active elements, such a thermoelectric cooler device, that is controlled by an algorithm that regulates a temperature distribution of an adjacent reaction-vessel according to a temperature distribution command trajectory and estimated reaction-vessel temperature distribution. Some embodiments include two active elements that are bilaterally applied to opposing sides of the reaction-vessel. In some embodiments, the estimated reaction-vessel temperature is determined based on a state of power electronics of the element and a temperature output of one or more sensors of a portion of the element and/or an ambient environment of the reaction-vessel. Methods of calibration of such systems utilizing a thermal calibrator as a proxy for the reaction-vessel are also provided herein.
G05D 23/19 - Control of temperature characterised by the use of electric means
F25B 21/02 - Machines, plants or systems, using electric or magnetic effects using Nernst-Ettinghausen effect
G01K 1/02 - Means for indicating or recording specially adapted for thermometers
G01K 13/024 - Thermometers specially adapted for specific purposes for measuring temperature of moving fluids or granular materials capable of flow of moving gases
G01N 1/42 - Low-temperature sample treatment, e.g. cryofixation
G01N 1/44 - Sample treatment involving radiation, e.g. heat
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
Methods and systems for estimating force and motor torque in a mechatronic system are provided herein. Such methods and system are suited for improved control of small-scale mechatronic system, particularly a syringe, valve, and cartridge loader or door opening/closing mechanism of a diagnostic assay system. The methods can compensate for friction and account for various second-order effects, thereby allowing for more accurate pressure estimation, thereby allowing improved syringe operation. The methods can further allow for improved estimation of force or motor torque to allow for improved control of an actuatable valve interfacing the sample cartridge and cartridge loader or door opening/closing system. Methods of calibrating such systems are also provided.
Provided are methods and compositions for activating oligonucleotide aptamer-deactivated DNA polymerases, comprising modifying the aptamer by uracil-DNA glycosylase enzymatic activity to reduce or eliminate binding of the oligonucleotide aptamer to the DNA polymerase, thereby activating DNA synthesis activity of the DNA polymerase in a reaction mixture. Mixtures for use in methods of the invention are also provided. In some aspects, the oligonucleotide aptamers are circular and comprise one or more deoxyuridine nucleotides providing for aptamer-specific recognition and modification of the circular aptamer by the uracil-DNA glycosylase enzymatic activity. Exemplary oligonucleotide aptamers, mixtures and methods employing uracil-DNA glycosylase enzymatic activity are provided. The methods can be practiced using kits comprising a DNA polymerase-binding oligonucleotide aptamer and at least one uracil-DNA glycosylase enzymatic activity having oligonucleotide aptamer-specific recognition to provide for specific modification of the aptamer by the uracil-DNA glycosylase enzymatic activity.
Provided are methods and compositions for activating oligonucleotide aptamer-deactivated DNA polymerases, comprising cleaving the aptamer by endonuclease V enzymatic activity to reduce or eliminate binding of the oligonucleotide aptamer to the DNA polymerase, thereby activating DNA synthesis activity of the DNA polymerase in a reaction mixture. Mixtures for use in methods of the invention are also provided. The oligonucleotide aptamers of the present invention are circular and comprise one or more deoxyinosine nucleotides providing for aptamer-specific recognition and cleavage of the circular aptamer by the endonuclease V enzymatic activity. Exemplary oligonucleotide aptamers, mixtures and methods employing endonuclease V enzymatic activity are provided. The methods can be practiced using kits comprising a DNA polymerase-binding oligonucleotide aptamer and at least one endonuclease V enzymatic activity having oligonucleotide aptamer-specific recognition to provide for specific cleavage of the aptamer by the endonuclease V enzymatic activity.
INTEGRATED PURIFICATION AND MEASUREMENT OF DNA METHYLATION AND CO-MEASUREMENT OF MUTATIONS AND/OR MRNA EXPRESSION LEVELS IN AN AUTOMATED REACTION CARTRIDGE
Methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulphonating the bound deaminated nucleic acid and/or simultaneously eluting and desulphonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.
Thermal control devices and methods to provide improved control, speed and efficiency in temperature cycling are provided herein. Such thermal control device and methods can include one or more active elements, such a thermoelectric cooler device, that is controlled by an algorithm that regulates a temperature distribution of an adjacent reaction-vessel according to a temperature distribution command trajectory and estimated reaction-vessel temperature distribution. Some embodiments include two active elements that are bilaterally applied to opposing sides of the reaction-vessel. In some embodiments, the estimated reaction-vessel temperature is determined based on a state of power electronics of the element and a temperature output of one or more sensors of a portion of the element and/or an ambient environment of the reaction-vessel. Methods of calibration of such systems utilizing a thermal calibrator as a proxy for the reaction-vessel are also provided herein.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
48.
LOSSY MECHATRONIC SYSTEMS AND METHODS OF ESTIMATION
Methods and systems for estimating force and motor torque in a mechatronic system are provided herein. Such methods and system are suited for improved control of small-scale mechatronic system, particularly a syringe, valve, and cartridge loader or door opening/closing mechanism of a diagnostic assay system. The methods can compensate for friction and account for various second-order effects, thereby allowing for more accurate pressure estimation, thereby allowing improved syringe operation. The methods can further allow for improved estimation of force or motor torque to allow for improved control of an actuatable valve interfacing the sample cartridge and cartridge loader or door opening/closing system. Methods of calibrating such systems are also provided.
An n phase encoder for use in a mechatronic system, including a movable element (104, 304) that applies a magnetic field with period S representing a total displacement, and a stationary support (201, 310) with n magnetic field sensors (202a-c, 312) mounted thereon and positioned on the stationary support (201, 310) so as to measure the magnetic field imparted by the movable element (104, 304), wherein n is greater than 1, the encoder being configured to obtain signals from the n magnetic field sensors (202a-c, 312) corresponding to the measurements of the magnetic field imparted by the movable element (104, 304), and to process n signals from the n magnetic field sensors (202a-c, 312) by utilizing a transformation matrix to determine a displacement of the movable element (104, 304), as well as associated signal processing and calibration methods.
G01D 5/244 - Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means generating pulses or pulse trains
G01D 5/14 - Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage
G01D 5/245 - Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means generating pulses or pulse trains using a variable number of pulses in a train
50.
ENCODERLESS MOTOR WITH IMPROVED QUANTIZATION AND METHODS OF USE AND CALIBRATION
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor to be determined from the sinusoidal signals by utilizing a transformation matrix or piece-wise algorithm applied in substantially linear portions of the sinusoidal signals without requiring use of additional hardware encoder or position sensors and without requiring noise-reduction or filtering of the signal.
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
Providing a website featuring scientific and technological information and advice in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; scientific and technological consulting services in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment. Providing a website featuring medical information and advice in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment and laboratory equipment; medical consulting services in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; medical diagnostic testing, monitoring and reporting services.
52.
DIAGNOSTIC ASSAY SYSTEM WITH REPLACEABLE PROCESSING MODULES AND REMOTE MONITORING
A biological sample processing apparatus having an enclosure and a plurality of sample processing modules held within an enclosure with a tiltable graphical user interface screen. In one aspect, the individual modules that are readily removable for repair, replacement or upgrade. Each module is configured to be independently operable and readily inserted into the enclosure for connection with a processing unit of the enclosure. Each module can include quick-release mechanisms so that the module can be readily removed and replaced manually or with minimal tools through the front of the enclosure without requiring substantial or total disassembly of the module or entire enclosure. In another aspect, the user interface screen can display identifying information, such as a barcode, that can be scanned by a user's portable device so as to monitor the progress of an assay remotely.
A biological sample processing apparatus having an enclosure and a plurality of sample processing modules held within an enclosure with a tiltable graphical user interface screen. In one aspect, the individual modules that are readily removable for repair, replacement or upgrade. Each module is configured to be independently operable and readily inserted into the enclosure for connection with a processing unit of the enclosure. Each module can include quick-release mechanisms so that the module can be readily removed and replaced manually or with minimal tools through the front of the enclosure without requiring substantial or total disassembly of the module or entire enclosure. In another aspect, the user interface screen can display identifying information, such as a barcode, that can be scanned by a user's portable device so as to monitor the progress of an assay remotely.
A biological sample processing apparatus having an enclosure and a plurality of sample processing modules held within an enclosure with a tiltable graphical user interface screen. In one aspect, the individual modules that are readily removable for repair, replacement or upgrade. Each module is configured to be independently operable and readily inserted into the enclosure for connection with a processing unit of the enclosure. Each module can include quick-release mechanisms so that the module can be readily removed and replaced manually or with minimal tools through the front of the enclosure without requiring substantial or total disassembly of the module or entire enclosure. In another aspect, the user interface screen can display identifying information, such as a barcode, that can be scanned by a user's portable device so as to monitor the progress of an assay remotely.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
(1) Diagnostic reagents for scientific use; reagents and kits comprised primarily of reagents for scientific and research use in connection with the amplification, analysis, labeling and detection of nucleic acids
(2) Medical diagnostic reagents; reagents and kits comprised primarily of reagents for medical, clinical, medical laboratory or medical diagnostic use in connection with the amplification, analysis, labeling and detection of nucleic acids
(3) Laboratory equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples, nucleic acid amplifiers and optical detectors; Laboratory equipment, namely, instrument systems, comprised of apparatus for nucleic acid amplification, analysis, labeling and detection, software for use in preparing and processing biological samples, cartridges, sample preparation land reaction containers and reaction vessels for preparing and processing biological samples, sold as a unit; apparatus for nucleic acid amplification, analysis, labeling and detection; Laboratory equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection; Laboratory devices for detecting genetic sequences; Diagnostic apparatus for the detection of pathogens for laboratory or research use; Scientific and research apparatus and instruments, namely, genetic sequencers for use in genetic analysis; Apparatus for DNA and RNA testing for scientific research purposes; Data processing apparatus
(4) Clinical and medical equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples, nucleic acid amplifiers and optical detectors; Clinical and medical equipment for diagnostic purposes, namely, instrument systems, comprised of apparatus for nucleic acid amplification, analysis, labeling and detection, software for use in preparing and processing biological samples, cartridges, sample preparation and reaction containers and reaction vessels for preparing and processing biological samples, sold as a unit; Clinical and medical equipment for diagnostic purposes, namely, apparatus for nucleic acid amplification, analysis, labeling and detection; Clinical and medical equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection; Clinical and medical diagnostic apparatus and instruments for detecting genetic sequences; Clinical and medical diagnostic apparatus for use in detecting pathogens; Clinical and medical diagnostic apparatus and instruments for use in genetic analysis; Apparatus for DNA and RNA testing for medical purposes; Clinical and medical diagnostic apparatus and instruments for the analysis of body fluids (1) Providing a website featuring information and advice in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; Consulting services in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; Analysis and evaluation of the goods of others to assure compliance with standards, industry standards, and objectively determinable standards; Analysis and evaluation of the services of others to assure compliance with standards, industry standards, and objectively determinable standards
(2) Providing a website featuring information and advice in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; Consulting services in the fields of laboratory testing, medical diagnostic testing, medical diagnostic equipment, and laboratory equipment; Medical diagnostic testing, monitoring and reporting services
56.
Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands
Provided are methods and compositions for activating oligonucleotide aptamer-deactivated DNA polymerases, comprising cleaving the aptamer by endonuclease V enzymatic activity to reduce or eliminate binding of the oligonucleotide aptamer to the DNA polymerase, thereby activating DNA synthesis activity of the DNA polymerase in a reaction mixture. Mixtures for use in methods of the invention are also provided. In some aspects, the oligonucleotide aptamer comprises one or more deoxyinosine nucleotides providing for aptamer-specific recognition and cleavage of the aptamer by the endonuclease V enzymatic activity. Exemplary oligonucleotide aptamers, mixtures and methods employing endonuclease V enzymatic activity are provided. The methods can be practiced using kits comprising a DNA polymerase-binding oligonucleotide aptamer and at least one endonuclease V enzymatic activity having oligonucleotide aptamer-specific recognition to provide for specific cleavage of the aptamer by the endonuclease V enzymatic activity.
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Diagnostic reagents for scientific use; reagents and kits
comprised primarily of reagents for scientific and research
use in connection with the amplification, analysis, labeling
and detection of nucleic acids. Medical diagnostic reagents; reagents and kits comprised
primarily of reagents for medical, clinical, medical
laboratory or medical diagnostic use in connection with the
amplification, analysis, labeling and detection of nucleic
acids. Laboratory equipment, namely, a device for bioanalysis
incorporating a cartridge for sample preparation, nucleic
acid purification, nucleic acid amplification and optical
detection; laboratory equipment, namely, reaction vessels,
fluidic blocks for preparing and processing biological
samples, cartridges for preparing and processing biological
samples. Medical equipment, namely, a device for bioanalysis
incorporating a cartridge for sample preparation, nucleic
acid purification, nucleic acid amplification and optical
detection; medical equipment, namely, reaction vessels,
fluidic blocks for preparing and processing biological
samples, cartridges for preparing and processing biological
samples.
58.
METHODS OF DIAGNOSING TUBERCULOSIS AND DIFFERENTIATING BETWEEN ACTIVE AND LATENT TUBERCULOSIS
Compositions and methods for detecting mycobacterium tuberculosis (MTB) infection in a patient suspected of being infected with mycobacterium tuberculosis and for distinguishing between active and latent tuberculosis infection are provided. The methods may also be used to monitor progression of MTB infection or to monitor treatment of MTB infected patients. Changes in the expression level of genes are used to aid in the diagnosis, prognosis and treatment of tuberculosis.
Compositions and methods for detecting Mycobacterium tuberculosis (MTB) infection in a patient suspected of being infected with Mycobacterium tuberculosis and for distinguishing between active and latent tuberculosis infection are provided. The methods may also be used to monitor progression of MTB infection or to monitor treatment of MTB infected patients. Changes in the expression level of genes are used to aid in the diagnosis, prognosis and treatment of tuberculosis.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
(1) Diagnostic reagents for scientific use; reagents and kits comprised primarily of reagents for scientific and research use in connection with the amplification, analysis, labeling and detection of nucleic acids.
(2) Medical diagnostic reagents; reagents and kits comprised primarily of reagents for medical, clinical, medical laboratory or medical diagnostic use in connection with the amplification, analysis, labeling and detection of nucleic acids.
(3) Laboratory equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification and optical detection; laboratory equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples.
(4) Medical equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification and optical detection; medical equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples.
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Laboratory equipment for scientific, medical, and generic research, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples Clinical and medical equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples
Automated oligonucleotide synthesis-compatible fluorescent dye phosphoramidite compounds, solid supports, and labeled polynucleotides incorporating the compounds are provided. The compounds allow universal incorporation of the fluorescent label into any position of the polynucleotide.
C07D 491/02 - Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups , , or in which the condensed system contains two hetero rings
G01N 33/542 - Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
63.
METHODS OF DETECTING SARS-COV-2, INFLUENZA, AND RSV
Compositions and methods for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), influenza A, influenza B, and respiratory syncytial virus (RSV) are provided.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
Automated oligonucleotide synthesis-compatible fluorescent dye phosphoramidite compounds, solid supports, and labeled polynucleotides incorporating the compounds are provided. The compounds allow universal incorporation of the fluorescent label into any position of the polynucleotide.
C07D 491/052 - Ortho-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring the oxygen-containing ring being six-membered
Automated oligonucleotide synthesis-compatible fluorescent dye phosphoramidite compounds, solid supports, and labeled polynucleotides incorporating the compounds are provided. The compounds allow universal incorporation of the fluorescent label into any position of the polynucleotide.
C07D 491/02 - Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups , , or in which the condensed system contains two hetero rings
C09B 57/00 - Other synthetic dyes of known constitution
G01N 33/542 - Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
Providing a website featuring information and advice in the fields of laboratory testing and the use of laboratory equipment Providing a website featuring information and advice in the fields of medical diagnostic testing and the use of medical diagnostic equipment
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
Providing a website featuring information and advice in the fields of laboratory testing and the use of laboratory equipment Providing a website featuring information and advice in the fields of medical diagnostic testing and the use of medical diagnostic equipment
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
Providing a website featuring information and advice in the fields of laboratory testing and the use of laboratory equipment Providing a website featuring information and advice in the fields of medical diagnostic testing and the use of medical diagnostic equipment
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
Providing a website featuring information and advice in the fields of laboratory testing and the use of laboratory equipment Providing a website featuring information and advice in the fields of medical diagnostic testing and the use of medical diagnostic equipment
72.
METHODS OF DETECTING SARS-COV-2, INFLUENZA, AND RSV
Compositions and methods for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), influenza A, influenza B, and respiratory syncytial virus (RSV) are provided.
Compositions and methods for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), influenza A, influenza B, and respiratory syncytial virus (RSV) are provided.
Described herein are methods and compositions that provide highly efficient nucleic acid amplification and signal detection using a cycling probe. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.
Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR.
An analytic system for carrying out a chemical assay, the system having a bay with an opening on one side, the bay of a size and shape to enclose a cartridge carrying sample material to be analyzed, one or more mechanisms within the bay through which the cartridge and or material within the cartridge is influenced, a door of a size to cover the opening, a securing mechanism associated with the bay and the door, by which the door is secured when closed.
Thermal control devices adapted to provide improved control and efficiency in temperature cycling are provided herein. Such thermal control device can include a thermoelectric cooler controlled in coordination with another thermal manipulation device to control an opposing face of the thermoelectric cooler and/or a microenvironment. Some such thermal control devices include a first and second thermoelectric cooler separated by a thermal capacitor. The thermal control devices can be configured in a planar configuration with a means for thermally coupling with a planar reaction vessel of a sample analyzer for use in thermal cycling in a polymerase chain reaction of the fluid sample in the reaction vessel. Methods of thermal cycling using such a thermal control devices are also provided.
A lid apparatus for a multi-chambered container. The lid apparatus has a top-lid that is hingedly attached to a bottom-cap. The top-lid includes one or more openings for fluid filling multiple passages that extend from the bottom-cap. A lower bottom-cap includes welding features for welding to the multi-chambered container.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
Diagnostic reagents for scientific use; reagents and kits comprised primarily of reagents for scientific and research use in connection with the amplification, analysis, labeling and detection of nucleic acids; reagents and kits comprised primarily of reagents for clinical or medical laboratory use in connection with the amplification, analysis, labeling and detection of nucleic acids Medical diagnostic reagents; reagents and kits comprised primarily of reagents for medical or medical diagnostic use in connection with the amplification, analysis, labeling and detection of nucleic acids Laboratory equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples, nucleic acid amplifiers and optical detectors; Laboratory equipment, namely, instrument systems, comprised of apparatus for nucleic acid amplification, analysis, labeling and detection, recorded or downloadable software for use in preparing and processing biological samples, cartridges, sample preparation land reaction containers and reaction vessels for preparing and processing biological samples, sold as a unit; apparatus for nucleic acid amplification, analysis, labeling and detection for laboratory use; Laboratory equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection; Laboratory devices for detecting genetic sequences; Diagnostic apparatus for the detection of pathogens for laboratory or research use; Scientific and research apparatus and instruments, namely, genetic sequencers for use in genetic analysis; Apparatus for DNA and RNA testing for scientific research purposes; Data processing apparatus Clinical diagnostic equipment and medical diagnostic equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples, nucleic acid amplifiers and optical detectors used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens; Clinical and medical equipment for diagnostic purposes, namely, instrument systems, comprised of apparatus for nucleic acid amplification, analysis, labeling and detection, recorded or downloadable software for use in preparing and processing biological samples, cartridges, sample preparation and reaction containers and reaction vessels for preparing and processing biological samples, sold as a unit used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens; Clinical and medical equipment for diagnostic purposes, namely, apparatus for nucleic acid amplification, analysis, labeling and detection used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens; Clinical diagnostic equipment and medical diagnostic equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens; Clinical and medical diagnostic apparatus and instruments for detecting genetic sequences used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens; Clinical and medical diagnostic apparatus for use in detecting pathogens used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens; Clinical and medical diagnostic apparatus and instruments for use in genetic analysis used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens; Diagnostic apparatus for DNA and RNA testing for medical purposes used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens; Clinical and medical diagnostic apparatus and instruments for the analysis of body fluids used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens; Diagnostic apparatus for nucleic acid amplification, analysis, labeling and detection for medical purposes used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens Providing a website featuring information and advice in the fields of laboratory testing and the use of laboratory equipment; Consulting services in the fields of laboratory testing and the use of laboratory equipment; Analysis and evaluation of the goods of others to assure compliance with standards, industry standards, and objectively determinable standards; Analysis and evaluation of the services of others to assure compliance with standards, industry standards, and objectively determinable standards Providing a website featuring information and advice in the fields of medical diagnostic testing and the use of medical diagnostic equipment; Consulting services in the fields of medical diagnostic testing and the use of medical diagnostic equipment; Medical diagnostic testing, monitoring and reporting services
80.
Encoderless motor with improved granularity and methods of use
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with permanent magnets distributed radially about the rotor, the permanent magnets extending beyond the magnetic core, and sensors mounted to the substrate adjacent the permanent magnets. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an angular position of the rotor relative the substrate to be determined from linear portions of the sinusoidal signal without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02K 11/00 - Structural association of dynamo-electric machines with electric components or with devices for shielding, monitoring or protection
H02K 15/02 - Methods or apparatus specially adapted for manufacturing, assembling, maintaining or repairing of dynamo-electric machines of stator or rotor bodies
H02P 6/16 - Circuit arrangements for detecting position
H02K 29/08 - Motors or generators having non-mechanical commutating devices, e.g. discharge tubes or semiconductor devices with position sensing devices using magnetic effect devices, e.g. Hall-plates or magneto-resistors
H02K 21/22 - Synchronous motors having permanent magnets; Synchronous generators having permanent magnets with stationary armatures and rotating magnets with magnets rotating around the armatures, e.g. flywheel magnetos
Described herein are methods and compositions that provide highly efficient nucleic acid amplification and signal detection using a cycling probe. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.
Described herein are methods and compositions that make use of pseudo-complementary bases to reduce unwanted hybridization in assays to detect and/or quantify particular nucleotide sequences, as well as in nucleic acid sequencing protocols.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
83.
EXPONENTIAL BASE-3 AND GREATER NUCLEIC ACID AMPLIFICATION WITH CYCLING PROBE
Described herein are methods and compositions that provide highly efficient nucleic acid amplification and signal detection using a cycling probe. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.
Described herein are methods and compositions that make use of pseudo-complementary bases to reduce unwanted hybridization in assays to detect and/or quantify particular nucleotide sequences, as well as in nucleic acid sequencing protocols.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
85.
METHODS AND COMPOSITIONS FOR NUCLEIC ACID ISOLATION
In various embodiments methods detecting and/or quantifying a target analyte using immuno-PCR and optionally nucleic acid amplification are provided. In certain embodiments the methods utilize a cartridge for performing immuno-PCR to detect and/or quantify one or more target analytes, and optionally detecting and/or quantifying a nucleic acid, where the cartridge comprises a sample receiving chamber; a chamber comprising a matrix material that acts as a filter and/or a DNA binding agent; a temperature controlled channel or chamber; and a plurality of chambers containing reagents and/or buffers for performing immuno-PCR, where the plurality of chambers comprises a chamber containing a capture antibody that binds the analyte that is to be detected; the plurality of chambers comprises a chamber containing a detection antibody where said detection antibody is optionally attached directly or indirectly to a signal DNA; the plurality of chambers comprises a chamber containing a PCR master mix; the plurality of chambers comprises a chamber containing primers for amplifying all or a region of said signal DNA; and the plurality of chambers comprises a chamber containing a probe for detecting all or a region of said signal DNA.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
C12Q 1/6804 - Nucleic acid analysis using immunogens
The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Diagnostic reagents for scientific use; reagents and kits comprised primarily of reagents for scientific and research use in connection with the amplification, analysis, labeling and detection of nucleic acids; Reagents and kits comprised primarily of reagents for clinical or medical laboratory use in connection with the amplification, analysis, labeling and detection of nucleic acids Medical diagnostic reagents; Reagents and kits comprised primarily of reagents for medical or medical diagnostic use in connection with the amplification, analysis, labeling and detection of nucleic acids Laboratory equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection; Laboratory equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples Medical diagnostic equipment, namely, a device for bioanalysis incorporating a cartridge for sample preparation, nucleic acid purification, nucleic acid amplification, and optical detection used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, bacteria, and other pathogens; Medical diagnostic equipment, namely, reaction vessels, fluidic blocks for preparing and processing biological samples, cartridges for preparing and processing biological samples used in testing for the purpose of detecting and diagnosing medical conditions, diseases and disorders, genetic conditions, viruses, and bacteria, and other pathogens
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with a first set of permanent magnets distributed radially about the rotor to facilitate rotation of the rotor and a second set of permanent magnets on the rotor to facilitate determination of an absolute position of the rotor. The motor further includes first and second set of sensors for detection of the magnets of the inner and outer rings. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an absolute position of the rotor relative the substrate to be determined from the sinusoidal signals without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02P 7/025 - Arrangements for regulating or controlling the speed or torque of electric DC motors the DC motors being of the linear type the DC motors being of the moving coil type, e.g. voice coil motors
A remote monitoring system for medical data collection can include a data-flagging process embeds authorization and settings information into a file containing the test results. Such data flagging can occur at a medical device or testing site, and may be based in policy settings received from a remote system. A file containing the test results can also include data category information that can be used to protect sensitive information by preventing such information from being communicated to the wrong server.
G06F 15/173 - Interprocessor communication using an interconnection network, e.g. matrix, shuffle, pyramid, star or snowflake
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
H04L 67/12 - Protocols specially adapted for proprietary or special-purpose networking environments, e.g. medical networks, sensor networks, networks in vehicles or remote metering networks
G16H 40/67 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for remote operation
G16H 40/40 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the management of medical equipment or devices, e.g. scheduling maintenance or upgrades
Solid supports modified with pectins derivatives are provided. The solid supports are useful in nucleic acid isolation, separation, and detection methods.
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with a first set of permanent magnets distributed radially about the rotor to facilitate rotation of the rotor and a second set of permanent magnets on the rotor to facilitate determination of an absolute position of the rotor. The motor further includes first and second set of sensors for detection of the magnets of the inner and outer rings. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an absolute position of the rotor relative the substrate to be determined from the sinusoidal signals without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02K 29/08 - Motors or generators having non-mechanical commutating devices, e.g. discharge tubes or semiconductor devices with position sensing devices using magnetic effect devices, e.g. Hall-plates or magneto-resistors
H02K 11/215 - Magnetic effect devices, e.g. Hall-effect or magneto-resistive elements
H02P 6/16 - Circuit arrangements for detecting position
94.
MOTOR HAVING INTEGRATED ACTUATOR WITH ABSOLUTE ENCODER AND METHODS OF USE
A DC electric motor having a stator mounted to a substrate, the stator having a coil assembly having a magnetic core, a rotor mounted to the stator with a first set of permanent magnets distributed radially about the rotor to facilitate rotation of the rotor and a second set of permanent magnets on the rotor to facilitate determination of an absolute position of the rotor. The motor further includes first and second set of sensors for detection of the magnets of the inner and outer rings. During operation of the motor passage of the permanent magnets over the sensors produces a substantially sinusoidal signal of varying voltage substantially without noise and/or saturation, allowing an absolute position of the rotor relative the substrate to be determined from the sinusoidal signals without requiring use of an encoder or position sensors and without requiring noise-reduction or filtering of the signal.
H02K 29/08 - Motors or generators having non-mechanical commutating devices, e.g. discharge tubes or semiconductor devices with position sensing devices using magnetic effect devices, e.g. Hall-plates or magneto-resistors
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
Methods and cleaning compositions for reduction of nucleic acid contamination on surfaces, in air, and in solutions using modified pectin are provided.
C11D 11/00 - Special methods for preparing compositions containing mixtures of detergents
C11D 17/04 - Detergent materials or soaps characterised by their shape or physical properties combined with or containing other objects
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
A61L 101/50 - Polysaccharides or derivatives thereof
A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.
A lid apparatus for a multi-chambered container. The lid apparatus has a top-lid that is hingedly attached to a bottom-cap. The top-lid includes one or more openings for fluid filling multiple passages that extend from the bottom-cap. A lower bottom-cap includes welding features for welding to the multi-chambered container. The bottom-cap further includes one or more auxiliary ports for injecting a reagent when the lid apparatus is in a closed configuration sealingly attached to the multi-chambered sample container.
A lid apparatus for a multi-chambered container. The lid apparatus has a top-lid that is hingedly attached to a bottom-cap. The top-lid includes one or more openings for fluid filling multiple passages that extend from the bottom-cap. A lower bottom-cap includes welding features for welding to the multi-chambered container. The bottom-cap further includes one or more auxiliary ports for injecting a reagent when the lid apparatus is in a closed configuration sealingly attached to the multi-chambered sample container.
A handling system for high throughput processing of a large volume of biological samples is provided herein. Such systems can include an array support assembly that supports multiple diagnostic assay modules in an array having at least two dimensions, a loader that loads multiple diagnostic assay cartridges within the multiple diagnostic assay modules. The array support assembly can be movable relative the loader to facilitate loading and unloading so as to provide more efficient processing.
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
