Abstract

The present invention relates to a composite for a thermal block of a thermal cycler. The composite has both low specific heat characteristics and remarkably improved thermal conductivity, and thus, when the temperature of a thermal block, which is manufactured using the composite, rises and falls, deviation in temperature change rate between a plurality of unit thermal blocks and/or in different areas in a unit thermal block can be remarkably reduced such that a PCR thermal cycler including the thermal block can ensure reliability even in ultrafast PCR.

IPC Classes  ?

• C09K 5/14 - Solid materials, e.g. powdery or granular
• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
• C22C 13/00 - Alloys based on tin
• B22F 1/054 - Nanosized particles

Abstract

Aphanomyces invadansAphanomyces invadans and/or internal transcribed spacer 1 (ITS1) thereof, a composition for detecting epizootic ulcerative syndrome comprising same, a detection kit therefor, and a method for detecting epizootic ulcerative syndrome using same.

IPC Classes  ?

• C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
• C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Abstract

The present invention relates to a composition for detecting a white spot syndrome virus and a method for detecting same and, particularly, to a pair of primers for amplifying VP664 and/or VP28, which are genes expressing envelope proteins of a white spot syndrome virus, a composition for detecting a white spot syndrome virus comprising same, a kit for detection, and a method for detecting a white spot syndrome virus using same.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

15/223/2211 ≤ 0.2

IPC Classes  ?

• H01B 1/02 - Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of metals or alloys
• H01B 1/22 - Conductive material dispersed in non-conductive organic material the conductive material comprising metals or alloys
• B82Y 40/00 - Manufacture or treatment of nanostructures

Abstract

An electromagnetic shielding composition according to the present invention is directed to an electromagnetic shielding composition capable of forming an electromagnetic shielding film with excellent electromagnetic shielding capacity and flexibility and, specifically, comprises: a core-shell structured metal nanowire comprising a core containing copper and a shell containing silver on the core; and plate-like metal particles selected from plate-like silver particles and silver-coated plate-like copper particles.

IPC Classes  ?

• H05K 9/00 - Screening of apparatus or components against electric or magnetic fields

Abstract

The present invention relates to a composition for administering a double-stranded oligonucleotide structure using an ultrasonic nebulizer. The method allows the double-stranded oligonucleotide, according to the present invention, which forms self-assembled nanoparticles having a neutral charge of 90 nm in an aqueous solution, to maintain the same concentration, molecular weight, purity, nanoparticle size, and osmolality as the undiluted solution material. In addition, the method can maintain target gene suppression ability without cytotoxicity and can deliver drugs specifically to the nasal cavity and lungs, thus allowing the present invention to be utilized in the prevention or treatment of respiratory viral infections including COVID-19, pulmonary fibrosis caused by viral infections, or respiratory diseases.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61K 47/60 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• A61K 47/58 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
• A61K 47/54 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
• A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
• A61K 9/00 - Medicinal preparations characterised by special physical form
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61K 31/7088 - Compounds having three or more nucleosides or nucleotides

Abstract

The present invention relates to a composition for alleviating hair graying, promoting hair growth and/or preventing or alleviating hair loss, comprising double-stranded miRNA as an active ingredient and, more specifically, to: miR-3139, miR-3189, miR-3199 or miR-8485, which are double-stranded miRNA; a double-stranded oligonucleotide structure comprising same; or a pharmaceutical or cosmetic composition for alleviating hair graying, promoting hair growth and/or preventing or alleviating hair loss, comprising nanoparticles containing the structure as an active ingredient. A composition according to the present invention can prevent hair graying and reduce the rate of progress thereof by activating melanocytes and promoting melanogenesis and can allow the hair already affected by graying to be improved to a state before graying. In addition, the composition promotes the activation of melanocytes and the proliferation of dermal papilla cells and keratinocytes that are present in hair follicles, and induces outer root sheath development and hair growth so that the effects of promoting hair growth and/or preventing hair loss can be exhibited.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61K 9/51 - Nanocapsules
• A61K 47/60 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• A61K 47/58 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
• A61K 47/54 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
• A61P 17/14 - Drugs for dermatological disorders for baldness or alopecia
• A61K 8/60 - Sugars; Derivatives thereof

Abstract

The present invention relates to an adapter for mounting a conductive pipette, a sample tube opening/closing device, and an automatic sample analysis system and, more specifically, to an adapter for mounting a conductive pipette, a sample tube opening/closing device, and an automatic sample analysis system, in which dispensing of a liquid sample and extraction, amplification and testing of nucleic acids are integrally carried out. In the present invention, disclosed is a sample tube opening/closing device comprising: a housing (110) that forms an inner space isolated from the outside and includes a door (120) for moving in/out a multi-well plate (20) for biological samples, including a plurality of sample tubes (10) in which biological samples are accommodated; and a sample tube opening/closing part that is installed in the inner space to be spaced apart from the multi-well plate (20) for biological samples and automatically opens and closes the sample tubes (10).

IPC Classes  ?

• B01L 3/02 - Burettes; Pipettes

Abstract

The present invention relates to a method for isolating a nucleic acid by using: a binding buffer containing acetic acid or phosphoric acid; and the pH difference between binding and washing buffers, and an elution buffer, wherein silica magnetic particles are added to a biological sample including a nucleic acid to which a binding buffer containing acetic acid, phosphoric acid, etc., has been added, thereby binding the nucleic acid to the magnetic particles, after which a magnetic field is applied to separate the magnetic particles, and the nucleic acid is purified in a basic pH elution buffer. The method uses the pH difference, and can isolate the nucleic acid more quickly and efficiently than centrifugation or gravity separation. In addition, since ethanol is not used, high-purity nucleic acid can be isolated and purified to obtain more accurate results in subsequent experiments.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The present invention relates to a nucleic acid amplification test apparatus, and an automatic sample analysis system having same and, more particularly, to a nucleic acid amplification test apparatus in which separation and purification, dispensation, amplification, and testing of samples are performed integrally, and an automatic sample analysis system having same. Disclosed in the present invention is a nucleic acid amplification test apparatus comprising: a housing (30) having an inner space (S1) isolated from the outside; a multi-well plate insertion unit (100) into which a multi-well plate (40) having a plurality of reaction tubes (1) accommodating a target nucleic acid solution is inserted through an automatic purification and dispensing apparatus (10); a sealing plate insertion unit (200) into which a sealing plate (50) having a sealing means for sealing the inlets of the plurality of reaction tubes (1); a fluorescence detection unit (400) disposed on an upper side of the sealing plate insertion unit (200) and detecting a target nucleic acid in the reaction tubes (1); and a temperature control block (500) disposed on a lower side of the multi-well plate insertion unit (100) and controlling the temperature of the reaction tubes (1), wherein the reaction tubes (1) are moved relatively so as to be adjacent to the sealing means to be then sealed by the sealing means.

IPC Classes  ?

• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
• B01L 3/02 - Burettes; Pipettes
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

The present invention relates to: a double-stranded oligonucleotide which can highly specifically and efficiently inhibit the proliferation of Severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2), preferably a double-stranded oligonucleotide comprising a sequence in the form of RNA/RNA, DNA/DNA or a DNA/RNA hybrid; a double-stranded oligonucleotide structure and nanoparticles comprising the double-stranded oligonucleotide; and a use thereof for treating COVID-19.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61K 9/14 - Particulate form, e.g. powders
• A61K 9/19 - Particulate form, e.g. powders lyophilised
• A61P 31/14 - Antivirals for RNA viruses

Abstract

The present invention relates to: a double-stranded oligonucleotide which can highly specifically and efficiently inhibit amphiregulin expression, preferably a double-stranded oligonucleotide comprising a sequence in the form of RNA/RNA, DNA/DNA or a DNA/RNA hybrid; and a use, of a double-stranded oligonucleotide structure and nanoparticles comprising the double-stranded oligonucleotide, for preventing or treating obesity.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61K 47/60 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• A61K 47/58 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
• A61K 47/54 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
• A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61K 9/19 - Particulate form, e.g. powders lyophilised
• A61P 3/04 - Anorexiants; Antiobesity agents

Abstract

The present invention relates to a trackable precise-sample-injection device for a multi-well plate and, more specifically, to a trackable precise-sample-injection device for a multi-well plate, the device being capable of: preventing cross-contamination that can occur when many samples are manually put into a multi-well plate, and a diagnosis error caused by the injection of a sample into the wrong well or repeated injection; and simply recording and tracking information about wells into which samples are injected. The present invention relates to a trackable precise-sample-injection device for a multi-well plate, the device being capable of being efficiently used for a pooling inspection, and the like by injecting a plurality of samples into a multi-well plate.

IPC Classes  ?

• B01L 3/02 - Burettes; Pipettes
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

The present invention relates to a composition using a urine sample for diagnosis of a kidney disease and, more specifically, to a urine test composition and a kit for diagnosis of a kidney disease, each comprising an agent specifically detecting amphiregulin from a urine sample, and a method for diagnosing a kidney disease by using the composition. According to the present invention, not only is there an advantage of being able to conveniently diagnose a kidney disease in a non-invasive manner using a urine sample for a kidney disease to which early diagnosis is important, but also the application of the present invention to a patient diagnosed with chronic renal failure can advantageously predict the plausibility of progression into end-stage renal failure in advance.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
• G01N 33/53 - Immunoassay; Biospecific binding assay; Materials therefor

Abstract

The present invention relates to a double-stranded oligonucleotide capable of inhibiting CTGF expression with a very specific and high efficiency, a double-stranded oligonucleotide structure and nanoparticles comprising the double-stranded oligonucleotide, and a use thereof in preventing or treating of fibrotic or respiratory diseases.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61K 9/19 - Particulate form, e.g. powders lyophilised
• A61P 11/00 - Drugs for disorders of the respiratory system
• A61P 43/00 - Drugs for specific purposes, not provided for in groups
• B82Y 5/00 - Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

Abstract

The present invention relates to an electric grill capable of precisely controlling the temperature of a grilling plate by directly applying a heating paste including carbon nanotubes to the bottom of the grilling plate. The present invention comprises: the grilling plate on which food to be cooked is placed; a heating body that is applied so as to be arranged on the lower portion of the grilling plate so as to heat the grilling plate; a heat insulating material which is disposed to be spaced apart from the heating body at a predetermined distance so as to block heat conduction to the outside; and a temperature sensor which is disposed between the heating body and the heat insulating material so as to measure the temperature of an upper plate, wherein a pair of electrodes for supplying electricity to the heating body are arranged along opposite edges of both sides of the heating body, and a heating region can be divided and controlled by dividing and combining the pair of electrodes, the heating body, the coating thickness, and the like.

IPC Classes  ?

• A47J 37/06 - Roasters; Grills; Sandwich grills
• H05B 1/02 - Automatic switching arrangements specially adapted to heating apparatus
• H05B 3/03 - Electrodes
• H05B 3/16 - Heating elements characterised by the composition or nature of the materials or by the arrangement of the conductor the conductor being mounted on an insulating base

Abstract

A conductive paste composition according to the present invention comprises: a silver-coated copper nanowire with a core-shell structure; a binder mixture containing a silicone resin binder and a hydrocarbon-based resin binder; and an organic solvent, and thus has low sheet resistance and can endure high temperatures, thereby achieving excellent conductivity and electromagnetic wave shielding characteristics. Furthermore, the conductive paste can be easily manufactured into a planar conductive film through a simple process, and the conductive film can be widely used in various fields, such as electromagnetic wave shielding, solar cell electrodes, electronic circuits, antennas, and the like.

IPC Classes  ?

• H01B 1/22 - Conductive material dispersed in non-conductive organic material the conductive material comprising metals or alloys
• H01B 5/14 - Non-insulated conductors or conductive bodies characterised by their form comprising conductive layers or films on insulating-supports
• C08K 3/08 - Metals
• C08L 1/02 - Cellulose; Modified cellulose
• C08L 33/00 - Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters,; Compositions of derivatives of such polymers
• C08J 5/18 - Manufacture of films or sheets

Abstract

The present invention relates to a system structure capable of detecting, in real time, nucleic acid extraction and amplification reactions and amplified products in an apparatus for implementing a polymerase chain reaction, the system structure comprising: a nucleic acid extraction cartridge for extracting nucleic acids from a biological sample with a nucleic acid extraction reagent stored therein, or preparing a PCR premix by additionally mixing the nucleic acids with a dried product of the polymerase chain reaction; a PCR plate which is inserted into the nucleic acid extraction cartridge and coupled thereto such that a flow path is connected therebetween, and which receives a nucleic acid solution or a PCR premix, which is extracted from the nucleic acid extraction cartridge, and dispenses and accommodates same in at least one reaction well having accommodated therein dried primers/probes, or a dried PCR product comprising primers/probes; a temperature control module provided on the PCR plate and comprising a pair of heating blocks which are adjacent to the reaction well (W) and apply different temperatures; and a scanning module provided below the PCR plate to scan the concentration of a reaction product amplified in the reaction well (W).

IPC Classes  ?

• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices

Abstract

The present invention pertains to: a double-stranded oligonucleotide construct having a structure in which a hydrophilic substance and a hydrophobic substance are conjugated by a simple covalent bond or a linker-mediated covalent bond at both ends of a DKK1-specific double-stranded oligonucleotide to efficiently deliver the double-stranded oligonucleotide into cells; a nanoparticle which can be produced through self-assembly of the double-stranded oligonucleotide construct by a hydrophobic interaction in an aqueous solution; and a hair loss-preventing and hair growth-promoting composition containing the double-stranded oligonucleotide construct or the nanoparticle. A double-stranded oligonucleotide construct including a DKK1-specific double-stranded oligonucleotide; a nanoparticle; and a hair loss prevention or hair growth composition containing the double-stranded oligonucleotide construct or the nanoparticle as an active ingredient according to the present invention highly efficiently suppress the expression of DKK1 without side effects, and are remarkably effective for preventing hair loss and promoting hair growth, and can thus be very usefully used for a composition for preventing hair loss and promoting hair growth.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61K 9/14 - Particulate form, e.g. powders
• A61P 17/14 - Drugs for dermatological disorders for baldness or alopecia

Abstract

A sample concentrator tube having a heat-resistant planar heating element adhered thereto, an analysis device comprising same, and an analysis method using same, according to the present invention, have an effect capable of precisely controlling the temperature by heating the sample concentrator tube uniformly and rapidly to a target temperature for desorption, and of desorbing an adsorbed sample almost simultaneously in any part of an adsorbent by minimizing the local temperature difference of the adsorbent in the tube. In addition, it is possible to minimize chemical noise by preventing thermal denaturation of the adsorbent caused by overheating, and there is an advantage of excellent reproducibility as well as an effect of being inexpensive, economical, and excellent in energy efficiency.

IPC Classes  ?

• G01N 1/40 - Concentrating samples
• B01D 53/04 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography with stationary adsorbents
• H05B 3/20 - Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater
• H05B 3/40 - Heating elements having the shape of rods or tubes

Abstract

A heater integrated gas chromatography (GC) column device according to the present invention is capable of precisely and uniformly controlling a temperature by having a high thermal conductivity, and raising and lowering a temperature at a high speed by having a low thermal mass, such that a measuring time is significantly decreased. The GC column is in contact with a bobbin with a homogeneous temperature distribution, and thus a temperature is homogeneously distributed in each GC column. Further, the heater integrated GC column device according to the present invention has the above-described effects and may have a smaller size.

IPC Classes  ?

• G01N 30/30 - Control of physical parameters of the fluid carrier of temperature
• H05B 3/16 - Heating elements characterised by the composition or nature of the materials or by the arrangement of the conductor the conductor being mounted on an insulating base
• H05B 3/14 - Heating elements characterised by the composition or nature of the materials or by the arrangement of the conductor characterised by the composition or nature of the conductive material the material being non-metallic
• G01N 30/02 - Column chromatography

Abstract

The present invention relates to: a double stranded oligonucleotide construct having a structure of the form in which a hydrophilic material and a hydrophobic material are conjugated by a simple covalent bond or a linker-mediated covalent bond at both ends of a double stranded oligonucleotide in order to efficiently deliver an androgen receptor specific oligonucleotide into a cell; a nanoparticle which can be produced by self-assembling the double stranded oligonucleotide constructs in an aqueous solution by hydrophobic interaction; and a composition for preventing hair loss or for promoting hair growth comprising the double stranded oligonucleotide construct. The double stranded oligonucleotide construct comprising an androgen receptor specific oligonucleotide, and the composition for preventing hair loss or for promoting hair growth comprising the same as an active ingredient, according to the present invention, can inhibit the expression of an androgen receptor with high efficiency without side effects, and thus can have an excellent effect on the prevention of hair loss, especially androgenic hair loss, alopecia areata, and telogen alopecia and the promotion of hair growth.

IPC Classes  ?

• A61K 47/60 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• A61K 47/58 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
• A61K 47/54 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
• A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61P 17/14 - Drugs for dermatological disorders for baldness or alopecia
• A61K 8/60 - Sugars; Derivatives thereof
• A61K 8/86 - Polyethers
• A61K 8/81 - Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds

Abstract

The present invention relates to a probe having octamine or octamine derivative bound thereto in addition to a reporter and a quencher to thus allow the quencher to more effectively suppress light emitted by the reporter, and to uses of the probe. When the probe according to the present invention is utilized, octamine or octamine derivative bound to the probe effectively suppresses the light emitted by the quencher for the reporter, and results in effects such as i) a reduction in base fluorescence, ii) an increase in delta fluorescence, and iii) a decrease in the value of cycle at threshold, thus allowing the probe to be effectively used in a variety of real-time polymerase chain reactions requiring accuracy and sensitivity.

IPC Classes  ?

• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

The present invention relates to an extraction apparatus capable of simultaneously extracting target materials from multiple biological samples and, more particularly, to a target material extraction apparatus in which magnetic bar blocks can be replaced according to kinds of multi-well plates to be inserted into a cartridge. When used, the target material extraction apparatus of the present invention is operated in such a manner that among various multi-well plates having different numbers of wells, multi-well plates suitable for a use purpose are loaded into a cartridge within the apparatus and magnetic bar blocks equipped with magnetic bars suitable therefor are selected and loaded. Thus, the apparatus has the advantage of selectively applying various multi-well plates to one installment according to the number of samples from which a target material is extracted or to the amount of the target material to be extracted.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

The present invention relates to an ion guide for transferring ions to a mass spectrometer. The ion guide of the present invention is characterized in that a plurality of DC rings and a plurality of RF multi-pole rings with a plurality of electrodes are arranged so as to intersect with each other between an inlet into which the ions move and an outlet for transferring the ions, wherein the inner diameter of the DC rings is kept constant and the inner diameter of the RF multi-pole rings gradually decreases in a direction from the inlet to the outlet.

IPC Classes  ?

• H01J 49/06 - Electron- or ion-optical arrangements
• H01J 49/10 - Ion sources; Ion guns
• H01J 49/26 - Mass spectrometers or separator tubes

Abstract

An electric roasting pan according to the present invention comprises: a roasting pan; a heating layer being in surface contact with the roasting pan and comprising a carbon nanotube and a silicone-based adhesive; and an electrode in contact with the heating layer. The electric roasting pan has excellent thermal efficiency thanks to the minimal heat loss thereof, can reach a target temperature within a short time to reduce a preheating time, and affords excellent cooking quality. Furthermore, the electric roasting pan can substantially prevent the occurrence of temperature deviation across the area of the roasting pan during a temperature increase and is of high durability and safety.

IPC Classes  ?

• A47J 37/10 - Frying pans, e.g. frying pans with integrated lids or basting devices
• A47J 36/02 - Selection of specific materials, e.g. heavy bottoms with copper inlay or with insulating inlay
• H05B 3/14 - Heating elements characterised by the composition or nature of the materials or by the arrangement of the conductor characterised by the composition or nature of the conductive material the material being non-metallic
• H05B 3/20 - Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater

Abstract

The present invention relates to a double-stranded oligonucleotide which can highly specifically and efficiently inhibit an amphiregulin expression and, preferably, a double-stranded oligonucleotide comprising a sequence in the form of RNA/RNA, DNA/DNA or DNA/RNA hybrid, a double-stranded oligonucleotide structure comprising the double-stranded oligonucleotide, nanoparticles comprising the double-stranded oligonucleotide structure, and a fibrosis or respiratory disease preventive or therapeutic use thereof.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61P 11/00 - Drugs for disorders of the respiratory system
• A61P 11/06 - Antiasthmatics
• A61P 29/00 - Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]

Abstract

The present invention relates to the structure of an analysis plate applied to a high-speed polymerase chain reaction (PCR), and to a PCR analysis plate used for implementing an analysis of a real-time PCR, a real-time nested PCR and a post-PCR lateral flow hybridization reaction. The present invention is provided with: a check valve for enabling the maintaining of positive pressure when an elastic film expands into a convex form by having a solution pushed therein by the positive pressure; a lateral flow analysis module for analyzing a post-PCR follow-up PCR or lateral flow; and a shut-off valve enabling the controlling of the movement of the solution after each reaction ends. A high-speed PCR analysis plate may be provided whereby, by pressing, by means of a temperature-controllable heating block, the elastic film, which is in a convex form by the solution, of a PCR unit, a PCR solution may undergo rapid temperature circulation with minimum heat resistance, and a PCR dried material and a nucleic acid solution may be homogenized and mixed.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

A gas sample analysis apparatus and a gas sample analysis method, according to the present invention, can estimate a flow rate and a time enabling a gas sample to be concentrated to a gas sample concentration suitable for analysis, that is, a required concentration amount, thereby remarkably reducing total analysis time including gas sample concentration time, and can allow a suitable amount of a sample required for quantitative analysis to be concentrated and analyzed, thereby increasing analysis accuracy and preventing a problem in which overall analysis efficiency deteriorates.

IPC Classes  ?

• G01N 30/06 - Preparation
• G01N 1/22 - Devices for withdrawing samples in the gaseous state
• G01N 1/40 - Concentrating samples
• G01N 30/72 - Mass spectrometers

Abstract

The present invention relates to a double-helix oligonucleotide construct comprising a double-stranded miRNA and a composition for preventing or treating cancer comprising the same. More particularly, the present invention relates to a double-helix oligonucleotide construct comprising miR-544a characterized by a method that effectively inhibits the proliferation of cancer cells or induces a voluntary death of cancer cells, and an anticancer composition comprising the construct.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
• A61K 31/7125 - Nucleic acids or oligonucleotides having modified internucleoside linkage, i.e. other than 3'-5' phosphodiesters
• A61P 35/00 - Antineoplastic agents

Abstract

The present invention relates to a device which is easy to use and in which real-time reaction product detection through nucleic acid extraction from a biospecimen, a PCR reaction, and scanning of excitation light of various wavelengths and fluorescence corresponding thereto can be automated, various tests can be carried out in a single operation, and in particular, accurate results can be obtained in a short amount of time.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

A biosample collection device capable of accurately examining a biosample is disclosed. The biosample collection device comprises a housing, a cap part and a biosample treatment solution storing part. The cap part is fastened to the housing so as to be detachable therefrom, and has a biosample collection part for collecting a biosample. The biosample treatment solution storing part stores a biosample treatment solution, and is damaged when the cap part is fastened to the housing after the biosample is collected by the biosample collection part, so as to mix the biosample treatment solution with the biosample.

IPC Classes  ?

• A61B 10/02 - Instruments for taking cell samples or for biopsy
• C12M 1/26 - Inoculator or sampler
• G01N 1/04 - Devices for withdrawing samples in the solid state, e.g. by cutting
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

The present invention relates to a display device for a matrix-assisted laser desorption ionization mass spectrum. Specifically, the display device, according to the present invention, comprises: a storage unit which stores data including information on the mass-to-charge ratio (m/z) of matrix-derived ions for each matrix and an original mass spectrum which is a mass spectrum acquired by means of the matrix-assisted laser desorption ionization of an analyte; an input unit which receives, as input, information on a matrix material used when acquiring the original mass spectrum; a signal processing unit which receives the information on the matrix material inputted into the input unit, is linked with the storage unit, and according to the information on the matrix material inputted via the input unit, removes, from the original mass spectrum, a peak corresponding to the mass-to-charge ratio (m/z) of matrix-derived ions of the corresponding matrix material, thereby generating a noise-removed mass spectrum which is a mass spectrum having noise removed therefrom; and an output unit which receives the noise-removed mass spectrum from the signal processing unit and outputs same.

IPC Classes  ?

• G01N 27/64 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electric discharges, e.g. emission of cathode using wave or particle radiation to ionise a gas, e.g. in an ionisation chamber
• H01J 49/16 - Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission

Abstract

A sample plate for MALDI mass spectrometry, according to the present invention, enables separately positioning, by means of a plastic insulation plate, metal wiring and metal dots onto which an analyte sample is to be loaded, and electrically connecting same by means of a via or a metal portion, and thus the energy transferred into the plate when radiating a laser beam on the target (metal dots) may be reduced compared to a sample plate using a base metal, and thus laser energy may be concentrated on the target, and an effect may be achieved whereby heat loss is minimized.

IPC Classes  ?

• H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
• G01N 1/40 - Concentrating samples

Abstract

The present invention relates to a sample plate for matrix-assisted laser desorption/ionisation (MALDI) mass spectrometry, and a mass spectrometry method using the same. The sample plate according to the present invention comprises: a conductive substrate; and an organic matrix region which is formed on one surface of the conductive substrate, and in which two or more different organic matrices are arranged so as to be spaced apart from one another.

IPC Classes  ?

• H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Abstract

The present invention relates to a matrix-assisted laser desorption ionization mass spectrometry method and, specifically, a mass spectrometry method according to the present invention comprises the steps of: acquiring a mass spectrum of an analyte by performing matrix-assisted laser desorption ionization of the analyte, wherein a detection spectrum, which is the mass spectrum of the analyte, is acquired using each of two or more matrixes different from one another; and removing, from each detection spectrum, a peak of a corresponding matrix to obtain a matrix-removed spectrum, and then acquiring a corrected mass spectrum of the analyte on the basis of a matrix-removed spectrum for each of different matrixes.

IPC Classes  ?

• G01N 27/62 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electric discharges, e.g. emission of cathode
• H01J 49/16 - Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
• H01J 49/10 - Ion sources; Ion guns

Abstract

The present invention relates to an epoxy paste composition including silver-coated copper nanowires having a core-shell structure, and a conductive film including the same.

IPC Classes  ?

• C08K 7/02 - Fibres or whiskers
• C08L 63/00 - Compositions of epoxy resins; Compositions of derivatives of epoxy resins
• C08K 3/08 - Metals
• C08K 5/00 - Use of organic ingredients
• C09D 7/00 - Features of coating compositions, not provided for in group ; Processes for incorporating ingredients in coating compositions
• C09D 163/00 - Coating compositions based on epoxy resins; Coating compositions based on derivatives of epoxy resins
• C08J 5/18 - Manufacture of films or sheets

Abstract

Disclosed is a biological sample processing apparatus capable of facilitating application and release of a magnetic field. Such a biological sample processing apparatus comprises a pipette block, a pipette block forward and backward delivery unit, a pipette block vertical delivery unit, a magnetic field application unit, and a heating unit. The pipette block is coupled in such a manner that a plurality of pipettes for sucking or discharging biological samples in a multi-well plate can be detached, wherein wells are arranged in a matrix shape along the row and column directions in the multi-well plate. The pipette block forward and backward delivery unit moves the pipette block along the forward and backward directions which are process processing directions. The pipette block vertical delivery unit moves the pipette block along the vertical direction. The magnetic field application unit is disposed below the multi-well plate, and applies a magnetic field to some wells of the multi-well plate. The heating unit is disposed below the multi-well plate so as to be spaced apart from the magnetic field application unit, and heats some wells of the multi-well plate.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system

Abstract

The present invention relates to a double-stranded oligo RNA structure comprising double-stranded miRNA, and a composition for preventing or treating cancer, containing the same. More specifically, the present invention relates to an anti-cancer pharmaceutical composition, containing: a double-stranded oligo RNA structure comprising miR-3670, miR-4477a and miR-8078, and characterized by a method for effectively inhibiting cancer cell proliferation or inducing cancer cell apoptosis; and a pharmaceutically acceptable carrier.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links

Abstract

The present invention relates to a silver-coated copper nanowire having a core-shell structure by using a chemical reduction method, and a manufacturing method therefor and, more specifically, to: a method for manufacturing a silver-coated copper nanowire, comprising a step of manufacturing a copper nanowire by a chemical method, and then coating the surface of copper through a chemical reduction method by using a silver nitrate-ammonia complex solution and a reducing agent in order to prevent the oxidation of the copper nanowire; and a silver-coated copper nanowire, having a core-shell structure, manufactured by the method. In addition, costs can be reduced since the copper nanowire can be reproduced by reusing a solution. According to the present invention, since the oxidation of the silver-coated copper nanowire having a core-shell structure is prevented even in air or at a high temperature, electrical conductivity does not deteriorate, and thus the silver-coated copper nanowire having a core-shell structure is useful in the manufacture of an electromagnetic interference-shielding paste or a highly conductive paste, which requires a high electrical conductivity.

IPC Classes  ?

• B22F 9/24 - Making metallic powder or suspensions thereof; Apparatus or devices specially adapted therefor using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
• B22F 1/02 - Special treatment of metallic powder, e.g. to facilitate working, to improve properties; Metallic powders per se, e.g. mixtures of particles of different composition comprising coating of the powder

Abstract

The present invention relates to: a STAT3 gene specific siRNA; a highly efficient double-helical oligo RNA structure including the same; a pharmaceutical composition containing the same; and a use thereof and, more particularly, to: a STAT3 gene specific siRNA; a double-helical oligo RNA structure, which includes the same and has a structure in which hydrophilic materials and hydrophobic materials are bound to both ends of the double-helical RNA (siRNA), by using a simple covalent bond or a linker-mediated covalent bond, so as to be effectively transferred into a cell; nanoparticles capable of being produced, in an aqueous solution, by hydrophobic interactions between the double-helical oligo RNA structures; a method for producing the double-helical oligo RNA structure; and a pharmaceutical composition for preventing or treating cancers, especially breast cancer, skin diseases including psoriasis, or inflammatory diseases, containing the double-helical oligo RNA structure. According to the present invention, the STAT3 specific siRNA and the composition for treating cancers, skin diseases, or inflammatory diseases, containing the double-helical oligo RNA structure including the same can achieve a therapeutic effect on cancers, especially breast cancer, skin diseases including psoriasis, or inflammatory diseases by inhibiting the expression of STAT3 with high efficiency without causing side effects, and thus the present invention can be useful in treating breast cancer, skin diseases, or inflammatory diseases for which there currently is no suitable therapeutic agent.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 9/19 - Particulate form, e.g. powders lyophilised
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Abstract

The present invention relates to a pharmaceutical composition for treating cancer comprising, as an active ingredient, at least one miRNA selected from a group consisting of miR-3670, miR-4477a and miR-8078. The pharmaceutical composition for treating cancer according to the present invention has superior effects of suppressing proliferation of cancer cells and inducing destruction of cancer cells. Accordingly, the pharmaceutical composition of the present invention may be effectively used as an anticancer agent.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links

Abstract

The present invention relates to a binder-coupled carbon nanostructure nano-porous membrane and a manufacturing method therefor. More particularly, the present invention relates to a binder-coupled carbon nanostructure nano-porous membrane in which a carbon nano structure is deposited on one surface or both surfaces of a porous membrane support having micro- or nanopores, the same goes through a solution mixing polymer binder with a solvent, and a firing process is performed upon the same to thereby couple a porous membrane support and a carbon nanostructure; and a manufacturing method therefor.

IPC Classes  ?

• B01D 71/02 - Inorganic material
• B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
• B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus

Abstract

The present invention relates to a method for isolating and purifying a nucleic acid using a magnetic particle and, more specifically, to a method for isolating a nucleic acid from a biological sample using a magnetic particle comprising the steps of: (a) adding a magnetic particle having an average particle size of 50nm - 1μm to a biological sample including a nucleic acid to thereby combine an insoluble aggregate or a nucleic acid molecule with the magnetic particle; and (b) isolating the magnetic particle combined with the insoluble aggregate or the nucleic acid molecule using a magnetic field to thereby obtain the nucleic acid. The method for isolating a nucleic acid using a magnetic particle of the present invention can dramatically reduce the time required to isolate a nucleic acid compared to a prior method and completes purification in a short time using a silica magnetic particle even in a process after a nucleic acid or protein has been isolated, thereby having an advantage of being capable of completing the whole process in a much faster time compared to centrifugation or gravity separation, etc. and also improving the yield.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• G01N 33/553 - Metal or metal coated

Abstract

The present invention relates to a silver-coated copper nanowire and a preparation method therefor and, more specifically, is characterized by synthesizing a copper nanowire through a chemical method by using piperazine (C4H10N2) and/or hexamethylenediamine (C6H16N2), which are novel copper capping agents, and then coating the same with silver by using a chemical plating method in order to prevent the oxidation of the copper nanowire.

IPC Classes  ?

• B22F 1/00 - Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
• B22F 9/18 - Making metallic powder or suspensions thereof; Apparatus or devices specially adapted therefor using chemical processes with reduction of metal compounds
• B82B 3/00 - Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units

Abstract

The present invention relates to a novel siRNA, and a highly efficient double-stranded oligo RNA structure and nanoparticles comprising same, wherein the double-stranded oligo RNA structure is one in which a hydrophilic material and a hydrophobic material are conjugated to both terminals of the double-stranded RNA (siRNA) through a simple covalent bond or linker-mediated covalent bond to efficiently transport the double-stranded RNA structure into cells, and the double-stranded RNA structure may be changed into a nanoparticle form in an aqueous solution due to the hydrophobic interaction between the double-stranded RNA structures. The siRNA included in the double-stranded oligo RNA structure is preferably siRNA specific to a fibrosis- or respiratory disease-associated gene - particularly, amphiregulin or stratifin. Further, the present invention relates to a pharmaceutical composition comprising siRNA, and a highly efficient double-stranded oligo RNA structure and nanoparticles comprising same, as an active ingredient for preventing or treating fibrosis or respiratory diseases. Also, the present invention relates to a method for preventing or treating fibrosis or respiratory diseases including administering the pharmaceutical composition for preventing or treating fibrosis or respiratory diseases to a subject in need thereof.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61P 11/00 - Drugs for disorders of the respiratory system

Abstract

The present invention relates to a magnetic particle separating device, and a method of separating and purifying nucleic acid or protein using same. The device comprises: induction magnets (100); an induction magnet fixing portion (200) having induction magnet fixing holes (210) for fixing the induction magnets (100); and a body (300) in which entry holes (310) are formed into which tubes (T) are inserted. Thus, the application and removal of a magnetic field to and from the body is made very convenient, so that the device can be very advantageously used in the separation and purification of nucleic acid or protein.

IPC Classes  ?

• C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave

Abstract

The present invention relates to a micro chamber plate and, more particularly, to a micro chamber plate having a micro chamber hole formed using a liquid film such that, compared with injecting a sample using vacuum and/or centrifugal force, sample supply can not only be facilitated, but air bubbles, remaining sample, and the like included in the micro chamber hole can also be discharged efficiently, thereby enabling more accurate and efficient analysis.

IPC Classes  ?

• G01N 37/00 - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES - Details not covered by any other group of this subclass
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 1/36 - Embedding or analogous mounting of samples

Abstract

A ceramic paste composition according to the present invention comprises a carbon nanotube or a carbon nanotube-metal complex and a silicone adhesive, wherein the silicone adhesive contains 45-65 wt% of silicon dioxide and 0.1-10 wt% of a silanol group, and the ratio of a methyl group to a phenyl group is 1 wt% : 0.3-2.5 wt%. The ceramic paste composition according to the present invention is characterized in that the sheet resistance is low, thereby being capable of implementing excellent exothermic, shielding, absorptive, and conductive properties. In addition, despite a very high heat-generating temperature of 400 °C, compared to a general carbon-nanotube-based paste, the physical property of the paste composition is maintained at a stable level so that the inherent feature of the ceramic paste can be implemented. Furthermore, the ceramic paste can be easily produced as a sheet type conductive film through a simple process, and thus, can be applied in various fields such as heat-generating products, e.g. heat insulation, heating and the like, and electromagnetic shielding and absorbing products, electrodes, electronic circuits, antennas and the like.

IPC Classes  ?

• H01B 1/00 - Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors
• C04B 35/74 - Ceramic products containing macroscopic reinforcing agents containing shaped metallic materials
• C04B 35/80 - Fibres, filaments, whiskers, platelets, or the like
• C04B 41/45 - Coating or impregnating

Abstract

There is provided a liver cancer related specific siRNA and high efficiency double-stranded oligo RNA molecules containing the same. The double-stranded oligo RNA molecules have a structure in which hydrophilic and hydrophobic compounds are conjugated to both ends of the double-stranded oligo RNA molecules by a simple covalent bond or a linker-mediated covalent bond in order to be efficiently delivered into cells and may be converted into nanoparticles in an aqueous solution by hydrophobic interactions of the double-stranded oligo RNA molecules. The siRNA contained in the double-stranded oligo RNA molecules may be liver cancer related genes, particularly Gankyrin or BMI-1 specific siRNA. In addition, the present invention relates to a method of preparing the double-stranded oligo RNA molecules, and a pharmaceutical composition for preventing or treating cancer, particularly, liver cancer, containing the double-stranded oligo RNA molecules.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 31/7088 - Compounds having three or more nucleosides or nucleotides
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61P 35/00 - Antineoplastic agents

Abstract

There is provided a liver cancer related specific siRNA and high efficiency double-stranded oligo RNA molecules containing the same. The double-stranded oligo RNA molecules have a structure in which hydrophilic and hydrophobic compounds are conjugated to both ends of the double-stranded oligo RNA molecules by a simple covalent bond or a linker-mediated covalent bond in order to be efficiently delivered into cells and may be converted into nanoparticles in an aqueous solution by hydrophobic interactions of the double-stranded oligo RNA molecules. The siRNA contained in the double-stranded oligo RNA molecules may be liver cancer related genes, particularly ZBTB7A, YAP1 or CHD1L specific siRNA. In addition, the present invention relates to a method of preparing the double-stranded oligo RNA molecules, and a pharmaceutical composition for preventing or treating cancer, particularly, liver cancer, containing the double-stranded oligo RNA molecules.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61K 31/7088 - Compounds having three or more nucleosides or nucleotides
• A61P 35/00 - Antineoplastic agents

Abstract

The present invention relates to an oligonucleotide structure and a method for preparing the same and, more particularly, to an oligonucleotide structure in which a polymer compound is linked to an oligonucleotide via a covalent bond to improve in vivo stability of the oligonucleotide and cellular delivery efficiency of the oligonucleotide; and to a method for preparing the same. The oligonucleotide structure is improved into a homogenous material, thereby solving the problem in material verification due to polydispersion characteristics occurring when a hydrophilic material linked to the oligonucleotide is a synthetic polymer; the nucleotide structure is easy to synthesize compared with the existing process; and the size of a double helix oilgo-RNA structure can be accurately adjusted through the control of the number of repetitions of a hydrophilic material block, and thus, the gene expression regulation function of the oligonucleotide does not deteriorate through the synthesis of the optimized oligonucleotide structure, and the oligonucleotide can be delivered into cells at even a relatively low-concentration dosage. Therefore, the oligonucleotide structure of the present invention can be useful as a novel type oligonucleotide delivery system as well as a tool for treating cancers, infectious diseases, and the like.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Abstract

The present invention relates to a dengue virus-specific siRNA, a high-efficiency double helix oligo-RNA structure comprising the siRNA, and a composition for suppressing the proliferation of dengue virus comprising the RNA structure. The double helix oligo-RNA structure has a structure in which a hydrophilic material and a hydrophobic material are grafted on both ends of a double helix RNA (siRNA) via a simple covalent bond or a linker-mediated covalent bond so that the double helix oligo-RNA structure is efficiently delivered into cells, and can be transformed into a nanoparticle type by a hydrophobic interaction of the double helix RNA structures in an aqueous solution. The siRNA included in the double helix oligo-RNA structure specifically acts on the serotype of all dengue viruses. In addition, the present invention relates to a method for preparing the double helix oligo-RNA structure, and a pharmaceutical composition containing the double helix oligo-RNA structure for preventing or treating a dengue virus infection.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61P 31/12 - Antivirals

Abstract

The present invention relates to respiratory diseases, especially to gene specific siRNA related to idiopathic pulmonary fibrosis and chronic obstructive pulmonary disease (COPD), and a highly efficient double-helical oligo RNA structure containing the same, wherein the double-helical oligo RNA structure has a structure in which hydrophilic and hydrophobic materials are bonded at the both ends of the double-helical RNA (siRNA) using a simple covalent bond or a linker-mediated covalent bond to be effectively transferred into a cell, and is converted into nanoparticles by the hydrophobic interaction of the double-helical oligo RNA structure in a solution. It is desirable that the siRNA contained in the double-helical oligo RNA structure is a siRNA specific to a CTGF, Cyr61, or Plekho1, which are genes related to respiratory diseases, particularly idiopathic pulmonary fibrosis and COPD. In addition, the present invention relates to a method for producing the double-helical oligo RNA structure and a pharmaceutical composition containing the double-helical oligo RNA structure for preventing or treating respiratory diseases, particularly idiopathic pulmonary fibrosis and COPD.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61P 11/00 - Drugs for disorders of the respiratory system

Abstract

The present invention relates to an apparatus for automatically preparing cell-free proteins and a method for preparing proteins using the same, and more particularly, to an apparatus for automatically preparing cell-free proteins and a method for manufacturing proteins using the same, the apparatus comprising: a protein expression reaction unit including a reaction vessel comprising a plurality of dialysis tubes provided with a dialysis membrane by an open superstructure; a reaction temperature control unit for heating or cooling the protein expression reaction unit; a pipet array which includes a plurality of pipets and is capable of suctioning or discharging a solution by using the pipets; a pipet array movement unit capable of moving solutions by moving the pipet array up and down, back and forth, or left and right; a protein purification unit provided with a magnetic field application apparatus; and a multi-well plate provision unit capable providing a multi-well plate kit which supplies a solution used in the preparation of proteins. In addition, the present invention relates to the apparatus for automatically preparing cell-free proteins and the preparation method therefor, which has a pure target protein synthesizing function in which an affinity tag is removed, excluding a target protein in which an affinity tag is coupled, and a function for rapidly and automatically exchanging, by a desired elution solution, through multi-step dialysis, a leaching solution in which the resulting purified proteins are dissolved, with the desired elution solution for containing proteins such that a plurality of proteins can be simply and automatically prepared in mass. Moreover, the present invention relates to a reaction kit for automatically preparing cell-free proteins, comprising: a multi-well plate kit for storing a solution necessary for preparing proteins; and a dialysis tube provided with the dialysis membrane. The apparatus for automatically preparing cell-free proteins according to the present invention can automatically express and purify target proteins, and dialyze the proteins in the buffer solution for containing proteins thereby enabling the immediate use of the resulting purified proteins. Furthermore, the method for preparing proteins according to the present invention can continuously supply energy sources necessary for cell-free protein expressions by multi-leveled exchanges of a low molecular expression solution such that a maximum of five times more target proteins can be obtained compared with obtaining target proteins by using a conventional method for preparing cell-free proteins in an arrangement form, and since the preparation of pure target proteins by removing an unnecessary affinity tag using proteases when purifying target proteins by using magnetic particles, the present invention is very useful in protein production for industrial, medicinal and research purposes wherein circular proteins can be produced in which artificial sequences exerting negative influences on the activities and structures of a protein are removed.

IPC Classes  ?

• C12M 1/38 - Temperature-responsive control
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes

Abstract

The present invention relates to an amphiregulin-specific double-helical oligo-RNA which is a high-efficiency amphiregulin expression inhibitor capable of being useful for preventing or treating respiratory diseases. In addition, the present invention relates to: an amphiregulin-specific double-helical oligo-RNA structure in which hydrophilic and hydrophobic materials are simple covalently bonded or linker-mediated covalently bonded to improve the in vivo stability and cell delivery efficiency of the amphiregulin-specific double-helical oligo-RNA; and nanoparticles comprising the same. The amphiregulin-specific double-helical oligo-RNA structure according to the present invention can improve the stability of the amphiregulin-specific double-helical oligo-RNA and effectively enhance the cell membrane transduction function thereof without deteriorating the function of the amphiregulin-specific double-helical oligo-RNA. Therefore, it is possible to effectively deliver the amphiregulin-specific double-helical oligo-RNA into cells even with a relatively low concentration of dosage by employing the amphiregulin-specific double-helical oligo-RNA structure, thereby enabling the helpful use thereof for preventing or treating respiratory diseases.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links

Abstract

The present invention provides a novel azo compound having a quenching ability for the material that exhibits a luminescent phenomenon at an excited energy level, a quencher comprising the novel azo compound, a use of the quencher and a method for preparing the azo compound. The quencher according to the present invention may exhibit excellent characteristics in a wavelength absorption region.

IPC Classes  ?

• C07D 213/72 - Nitrogen atoms
• C09K 11/06 - Luminescent, e.g. electroluminescent, chemiluminescent, materials containing organic luminescent materials
• C09K 3/00 - Materials not provided for elsewhere

Abstract

The present invention relates to a kit for the detection of Epstein-Barr virus (EBV), comprising a primer and a probe to be used in detecting EBV using a real-time polymerase chain reaction (PCR). The present invention also relates to a detection method using the kit. The kit and the method of the present invention enable the detection of Epstein-Barr virus at a significantly low concentration in a quick and quantitative manner, thus preventing diseases caused by EBV.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Abstract

The present invention relates to a polymerase chain reaction primer and probe for detecting hepatitis B virus (HBV) and a detection kit and a method using same. When using the a for detecting HBV, according to the present invention, accurate detection of the hepatitis B virus is enabled, the kit has high sensitivity to HBV and can even confirm the genotype of the HBV, thereby providing the advantage of wide utilization in effective early diagnosis of the hepatitis B virus.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Abstract

The present invention relates to a method for preparing a nucleic acid with high precision, which is advantageous in that a nucleic acid polymerase is used to add a terminator to the nucleic acids to be used for analysis prior to the nucleic acid polymerization reaction such as PCR reaction or real time quantitative PCR reaction for detecting an infinitesimal nucleic acid, so that non-singular priming occurring competitively with amplification reaction of the target nucleic acid is basically eliminated, thus precisely detecting the infinitesimal target nucleic acid and precisely measuring the concentration of the target nucleic acid.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions

Abstract

The present invention relates to a heating paste comprising a carbon nanotube-metal composite, an organic binder, an adhesive agent and an organic solvent, and to a heating element comprising the heating paste. According to the present invention, excellent processability, low sheet resistance and reduced manufacturing cost can be realized, a large area device can be obtained, the formation of a pattern such as wiring in a local part is enabled, and the heating element can be utilized as heating products of various types.

IPC Classes  ?

• H01B 1/20 - Conductive material dispersed in non-conductive organic material
• H01B 1/02 - Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of metals or alloys
• B01F 11/02 - Mixing by means of ultrasonic vibrations

Abstract

The present invention relates to a composition for a hot-start reverse transcription reaction, as well as to a composition for a reverse transcription polymerase chain reaction (PCR) that contains the composition for a hot-start reverse transcription reaction. More particularly, the present invention relates to a reaction composition in which pyrophosphate and pyrophosphatase are added to an aqueous solution in a single reaction tube, said solution containing a buffer solution for reaction, MgCl2, four kinds of dNTPs, and reverse transcription polymerase. The present invention also relates to a composition for a hot-start reverse transcription reaction in which the reaction mixture is frozen or dried to achieve improved stability and long-term storability. The present invention also relates to a composition in which DNA polymerase is added to the above-described composition for a hot-start reverse transcription reaction to sequentially perform a hot-start reverse transcription reaction and PCR in a single reaction tube. The present invention further relates to a method for amplifying nucleic acids using the composition that sequentially performs a hot-start reverse transcription reaction and PCR in a single reaction tube. As compared to existing compositions for a hot-start reverse transcription reaction, the above-described composition for a hot-start reverse transcription reaction may prevent a non-specific reverse transcription reaction in a mixing process performed at room temperature and may enable a reverse transcription reaction for a target RNA at a reaction temperature to be selectively performed so as to perform PCR, and thus may enable RNA amplification to be performed with high sensitivity. Therefore, the above-described composition for a hot-start reverse transcription reaction can be used in a simple and effective manner in multiple reverse transcription PCR, real-time quantitative reverse transcription PCR, or the like.

IPC Classes  ?

• C12Q 1/42 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

Abstract

The present invention relates to an apparatus and method for automatically analyzing biological samples, which: dissolve a biological sample in a hydrolase and cell lysate, and dissolve nucleic acid in a solvent, and then attach same to magnetic particles and wash same; and then ultimately wash same using an organic solvent and dry the magnetic particles using a vacuum pump; and then liquate a target nucleic acid attached to the magnetic particles in a solvent; and then apply same to a vessel including a nucleic acid-based amplification reagent and mix same; and then adjust the temperature for amplification and simultaneously irradiate excitation light at a reactor and detect amplification in real time by measuring fluorescent light, deactivate the amplified product by using an infrared lamp after the amplification, and then acquire an image through electrophoresis; and use one apparatus for the entire process of analyzing molecular mass. Thus, after mounting biological sample kits, an electronically automated process can be implemented in order to perform a qualitative and quantitative inspection having a high level of accuracy and reproducibility. In particular, an infrared lamp is provided having a movable function in order to concentrate radiation on an amplified product and to deactivate a genetically amplified product. Thus, false-positives can fundamentally be prevented.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12M 1/38 - Temperature-responsive control
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave

Abstract

The present invention relates to a SAMiRNA-magnetic nanoparticle complex capable of efficiently delivering double-stranded RNA oligonucleotides and magnetic nanoparticle to cells and to a composition comprising the SAMiRNA-magnetic nanoparticle complex, capable of both diagnosing and treating diseases such as cancer. More particularly, the present invention relates to a SAMiRNA-magnetic nanoparticle complex wherein a double-stranded RNA oligonucleotide-polymer structure in which double-stranded RNA oligonucleotides and a hydrophilic material and a second hydrophobic material are connected by a simple covalent bond or a linker mediated covalent bond and a magnetic nanoparticle in which a first hydrophobic material is bonded to the surface of a magnetic material are formed into a core. The first hydrophobic material of the present invention performs a hydrophobic interaction with the second hydrophobic material of the double-stranded RNA oligonucleotide structure so as to form SAMiRNA-magnetic nanoparticle complexes having a uniform size. In addition, the hydrophilic material and the second hydrophobic material bonded in the double-stranded RNA oligonucleotide structure may improve in vivo stability of the double-stranded RNA oligonucleotides. A ligand which is additionally bonded to the structure may deliver a SAMiRNA-magnetic nanoparticle complex to a target cell even when a small amount of the ligand is applied, and a magnetic material of the magnetic nanoparticle may be used as an imaging agent for diagnosis.

IPC Classes  ?

• A61K 9/16 - Agglomerates; Granulates; Microbeadlets
• A61K 47/48 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives the non-active ingredient being chemically bound to the active ingredient, e.g. polymer drug conjugates
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
• A61K 31/7088 - Compounds having three or more nucleosides or nucleotides

Abstract

The present invention relates to a double-helical oligo-RNA structure and a method for manufacturing same, and more specifically, to a double-helical oligo-RNA structure in which a polymeric compound is connected to the double-helical oligo-RNA by means of a covalent bond to improve the biosatbility of the double-helical oligo-RNA and enhance cell delivery efficency, and to a method for manufacturing the structure. In the double-helical oligo-RNA structure having the optimum structure, as described above, a function of the double-helical oligo-RNA is not compromised, and the double-helical oligo-RNA can be delivered to cells even with a small dose of relatively low concentration due to the stability of the double-helical oligo-RNA and efficient enhancement of a cell membrane penetration function, can be efficiently enhanced, and thus can be useful as a tool for treating double-helical oligo-RNA in cancer and infectious diseases and as a novel form of a double-helical oligo-RNA delivery system.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links

Abstract

The present invention provides a double helix oligo-RNA structure in which target-specific ligands are bonded to the double helix oligo-RNA structure in which high molecular compounds for improving the delivery of double helix oligo-RNA that can be valuably used in treating diseases, specifically, cancer, are covalently bonded. The present invention also provides a method for preparing the RNA structure and a target-specific double helix oligo-RNA delivery technology through said structure. Nanoparticles constituted by the double helix oligo-RNA structure to which ligands are bonded may efficiently deliver double helix oligo-RNA to a target, and thus may exhibit an activity of double helix oligo-RNA structure in a target cell even with an administration of double helix oligo-RNA of relatively lower concentration. The nanoparticles may prevent the delivery of non-specific double helix oligo-RNA to other organs and cells, and therefore, can be valuably used not only as a tool for double helix oligo-RNA for treating various diseases, specifically, cancer, but also as a new type of double helix oligo-RNA delivery system. The present invention also relates to hybrid conjugates in which a hydrophilic material and a hydrophobic material are connected to both ends of antisense oligonucleotide(ASO) through covalent bond so as to achieve in vivo stability of the ASO, and to a method for preparing the hybrid conjugates and to nanoparticles constituted by the conjugates. The conjugates between ASO and macromolecules according to the present invention may improve in vivo stability of ASO, and therefore, can efficiently deliver ASO for treatment into cells and may exhibit an activity of ASO even with an administration of a relatively lower concentration.

IPC Classes  ?

• A61K 47/48 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives the non-active ingredient being chemically bound to the active ingredient, e.g. polymer drug conjugates
• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• A61K 9/16 - Agglomerates; Granulates; Microbeadlets
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Abstract

Provided are a method for manufacrturing a carbon nanotube-metal oxide composite, and a carbon nanotube-metal oxide composite manufactured thereby, the method comprising: dispersing carbon nanotubes in a reductive solvent to prepare a dispersion liquid; adding a co- reducing agent and a metal precursor to the dispersion liquid to prepare a mixture liquid; and performing heat treatment on the mixture liquid to coat the metal precursor on the carbon nanotubes in a metal oxide form, so that there can be provided a carbon nanotube-metal oxide composite where metal oxide particles of several nm to several tens of nm are uniformly dispersed in carbon nanotubes or combined with surfaces of the carbon nanotubes in a coating type.

IPC Classes  ?

• B82B 3/00 - Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
• B82Y 40/00 - Manufacture or treatment of nanostructures

Abstract

The present invention relates to a method for producing proteins. According to the method for producing proteins, using an automatic biological material refining apparatus provided with: a well plate kit; a heating part; and a magnetic field applying part, a plurality of target proteins may be obtained more quickly and simply compared to obtaining the target proteins using a protein expression/extraction method through an existing conventional cell culture. Thus, the present invention exhibits the advantage of a reproducible synthesis efficiency for proteins being improved since no deviation is generated between reaction wells.

IPC Classes  ?

• C07K 1/32 - Extraction; Separation; Purification by precipitation as complexes
• C07K 1/14 - Extraction; Separation; Purification
• C12N 15/09 - Recombinant DNA-technology
• C12M 1/18 - Multiple fields or compartments

Abstract

Provided is an oil filter capable of removing nanoparticles from oil. Specifically, the oil filter comprises a carbon nanostructuremetal composite nanoporous membrane in which one or both sides of a membrane support having micro- or nano-pores are coated with a carbon nanostructure-metal composite.

IPC Classes  ?

• B01D 71/02 - Inorganic material
• B01D 69/10 - Supported membranes; Membrane supports

Abstract

Provided is a method of purifying oil by which nano particles are effectively removed from the oil. According to the oil purifying method, oil is effectively purified at a high temperature using a carbon nanostructure-metal or -metal oxide composite nano porous membrane composed of a carbon nanostructure-metal composite.

IPC Classes  ?

• B01D 71/02 - Inorganic material
• C10M 175/00 - Working-up used lubricants to recover useful products

Abstract

The present invention relates to a primer and a probe for detecting Tamiflu resistance in new-strain influenza A/H1N1, and to a diagnostic method using same. A Tamiflu resistant C823T single nucleotide polymorphism discriminating primer set and probe for new-strain influenza A/H1N1 according to the present invention make a substantial contribution to the development of new antiviral drugs for Tamiflu resistant viruses and the like, since not only do they make it possible to accurately and efficiently analyse genetic variation in forms of life which have ribonucleic acid but they also make it possible to efficiently and accurately detect the Tamiflu resistant C823T single nucleotide polymorphism site in new-strain influenza A/H1N1 within a short time.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

The present invention relates to a primer for detecting pathogenic bacteria of oral bacterial infectious diseases, and a use thereof, and more specifically, to a primer for detecting pathogenic bacteria of oral bacterial infectious diseases, capable of qualitatively and quantitatively detecting pathogenic bacteria causing oral bacterial infectious diseases and having high species-specificity, a composition for detection, a kit, and a microarray. The primer for detecting pathogenic bacteria of oral bacterial infectious diseases according to the present invention has a high sensitivity and high species-specificity, can quickly and simply detect whether or not the periodontal tissue is infected by various bacteria, and can qualitatively and quantitatively detect pathogenic bacteria causing oral bacterial infectious diseases. In addition, the primer can be used as a tool for a bacteriological assessment of prognosis.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Abstract

The present invention relates to a method of identifying nucleic acid-containing object, more precisely a method of identifying nucleic acid-containing object which comprises the following steps: (1) preparing nucleic acid-containing object having the nucleotide sequence complementary to the nucleotide sequence of RNA-dual probe; (2) reacting the nucleic acid included in the object with the buffer containing the RNA-dual probe conjugated with a reporter and a quencher respectively and RNase; and (3) detecting fluorescence generated from the reporter. The method of identifying an object of the present invention provides labeling sensitivity 100 times as high as that of the conventional method using sequencing or labeling with fluorescent materials, takes advantages of shorter analysis time, facilitates different labeling on a variety of products according to fluorescent materials, and makes possible unlimited product administration by product group and batch in real production process by differentiating the nucleotide sequence of each oligonucleotide.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• G01N 33/52 - Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper

Abstract

The present invention relates to a composition for hot-start PCR, more precisely a composition for hot-start PCR characteristically prepared by adding the blocking oligonucleotide having the hydroxyl group at 3'-end blocked and the nucleotide sequence complementary to the primer to the conventional PCR composition containing reaction buffer, MgCl2, 4 kinds of dNTP, and DNA polymerase. The composition for hot-start PCR of the present invention can give more authentic PCR results by inhibiting smear band of PCR amplification product caused by non-specific reaction or primer-dimer formation at low temperature since the blocking oligonucleotide included therein can be dissociated at the lower temperature than melting temperature of the primer.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

Abstract

The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63rd glutamine (Q63), the 264th lysine (K264), the 295th lysine (K295), the 306th threonine (T306), the 346th glutamic acid (E346), the 408th proline (P408), the 438th histidine (H438), and the 454th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/54 - Transferases (2)
• C12N 15/63 - Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Abstract

The present invention relates to a method for determining the single nucleotide polymorphism of target genes using a real-time polymerase chain reaction, comprising the following steps: filling different tubes with allele-specific primers of target genes and carrying out real-time polymerase chain reaction using each probe common to target genes to obtain amplification products of the target genes; obtaining a Ct value from the allele-specific amplification products of each tube; obtaining a ΔCt value between alleles from the Ct value obtained in the previous step; and determining the single nucleotide polymorphism of the target genes from the obtained ΔCt value. The method for determining single nucleotide polymorphism according to the present invention may advantageously be used to detect a desired SNP in a short time.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

Abstract

This invention relates to a system for effectively removing organic compounds from air by a carbon nanotube-catalyst composite having the two functions of an adsorption/catalytic incineration agent. The carbon nanotube-catalyst composite simultaneously adsorbs the organic compounds and completely decomposes them by a catalytic reaction, and the optimal reaction active temperature by catalytic incineration is low. The carbon nanotube-catalyst composite has a large surface area and has high adsorption performance and catalytic decomposition activity, and is thus applicable to filters that use the methods of adsorption and/or catalytic incineration. The system for removing organic compounds from air includes an adsorption/catalytic incineration reactor including the carbon nanotube-catalyst composite to remove organic compounds from air.

IPC Classes  ?

• B01D 53/86 - Catalytic processes
• B01D 53/02 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography
• B01D 53/26 - Drying gases or vapours
• B82B 3/00 - Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units

Abstract

Disclosed is an automatic nucleic acid purification apparatus which can prevent pollution due to aerosol generated from a biological sample containing high concentration target nucleic acid when the biological sample containing the high concentration target nucleic acid is mixed with other biological sample containing low concentration target nucleic acid or not containing the target nucleic acid. Further, disclosed is an automatic nucleic acid purification apparatus which can be applied to all kinds of nucleic acid purification equipments for purifying a plurality of biological samples using a magnet rode or a multi-pipette block moving in two or three axial directions, and which can minimize pollution due to the aerosol generated from the biological sample containing high concentration target nucleic acid and also can obtain accurate results.

IPC Classes  ?

• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12S 3/20 - Removal of nucleic acids from intact or disrupted cells

Abstract

The present invention relates to an automatic real-time quantitative amplification system which can perform analysis of various biological samples, and more particularly to an automatic real-time quantitative amplification system in which a plurality of decks for respectively accommodating biological samples are put in a deck storing/transferring device, whereby it is possible to automatically analyze an amount or existence of a target substance containing a target nucleic acid in the biologic sample, such as a particular gene, a particular, a particular pathogenic bacterium and a particular protein, by amplifying the target nucleic acid purified by some processes of purification, purification after culture, or purification after reaction of the target substance contained in the biological sample and then checking an amount of the amplified target nucleic acid.

IPC Classes  ?

• G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
• G01N 33/53 - Immunoassay; Biospecific binding assay; Materials therefor
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12M 1/38 - Temperature-responsive control

Abstract

Provided are an oligonucleotide marker and a method of identifying a material using the same. The oligonucleotide marker makes it possible to analyze a trace amount of the material with high precision within a short time, has improved solubility in an oily solvent, and can improve a detection method such that the oligonucleotide marker can be detected within 2 hours. The oligonucleotide marker can label various products, including oil products and petroleum products, works of art and collections, and can also be used to conduct criminal investigations.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

Abstract

Provided are a method of manufacturing a carbon nanotube-platinum composite, including: dispersing carbon nanotubes in a reductive solvent to prepare a dispersion liquid; adding a stabilizer and a platinum precursor to the dispersion liquid to prepare a mixed solution; and heat-treating the mixed solution to reduce the platinum precursor, and a carbon nanotube-platinum composite manufactured by the method. The method is advantageous in that a carbon nanotube-platinum composite can be manufactured, in which platinum particles having a size of several nanometers are uniformly dispersed in carbon nanotubes and the size of platinum particles bonded with carbon nanotubes is uniform.

IPC Classes  ?

• B82B 3/00 - Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
• C01B 31/02 - Preparation of carbon; Purification
• C01G 55/00 - Compounds of ruthenium, rhodium, palladium, osmium, iridium, or platinum

Abstract

Provided is a low heat capacity composite for a thermal cycler. The low heat capacity composite of the present invention is a low heat capacity composite for a thermal cycler capable of overcoming difficulty in manufacture and reproducibility due to uniqueness of the existing PCR thermal cycler only. The low heat capacity composite of the present invention can reduce the cost of raw material and retain excellent heat property due to the improvement in low heat capacity and physical and mechanical properties, thereby remarkably shortening PCR reaction time and saving energy when used as a thermal block for a thermal cycler.

IPC Classes  ?

• C09K 5/14 - Solid materials, e.g. powdery or granular
• C22C 26/00 - Alloys containing diamond
• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices

Abstract

The present invention relates to a micro chamber plate, and more particularly, to an analytic micro chamber plate in which a plurality of reaction solutions including a primer or probe selectively reacting with a nucleic acid react with each other without cross-contamination to measure and analyze a fluorescence level in real-time so as to analyze biological sample solution including a large amount of nucleic acids. Also, the present invention relates to a method of manufacturing the analytic chamber plate. Also, the present invention relates to a method of manufacturing a micro chamber plate with a built-in sample used for manufacturing the analytic chamber plate. Also, the present invention relates to an apparatus set for manufacturing the micro chamber plate with a built-in sample.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• G01N 33/48 - Biological material, e.g. blood, urine; Haemocytometers
• G01N 1/16 - Devices for withdrawing samples in the liquid or fluent state with provision for intake at several levels

Abstract

The present invention relates to a primer and probe for detecting Mycobacterium tuberculosis and to a detection method using the same, and more specifically relates to a primer and probe adapted to detect genes of Mycobacterium tuberculosis present in biological samples and environmental samples, and to a method for detecting genes of Mycobacterium tuberculosis by means of a polymerase chain reaction using the same. When the present invention is employed, detection can be effected in real time more rapidly and accurately than with existing Mycobacterium tuberculosis detection methods, and a dried form of polymerase chain reaction mixture comprising the primer and probe has an improved storage period but the same performance as in the liquid state and hence can be used in a detection kit.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12R 1/32 - Mycobacterium

Abstract

The present invention relates to a hepatitis C virus gene-specific primer, to a probe, and to a detection method using same, and more particularly, to a primer for simultaneously diagnosing the hepatitis C virus gene by means of a polymerase chain reaction, to a probe, to a diagnostic kit including the primer and probe for an internal control group, and to a method for diagnosing the hepatitis C virus using the kit. According to the present invention, real-time detection that is faster and more accurate than existing hepatitis C virus detection methods is made possible, and as a dried polymerase chain reaction composition including the primer and probe has a longer storage time than one in solution form while having equal performance, it can be advantageously used in a kit for detecting the hepatitis C virus.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12N 15/51 - Hepatitis viruses
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

The present invention relates to a diagnostic primer for the hepatitis B virus, to a probe, and to a detection method using same, and more particularly, to a hepatitis B virus surface antibody S gene-specific primer, to a probe, and to a method for diagnosing the hepatitis B virus using the primer and probe. When the primer and probe of the present invention are used, a small amount of hepatitis B viral DNA present in a biological sample can be quickly and accurately detected, stability in storage can be improved by means of the provision of a dried kit, and highly reproducible results can be obtained.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12N 15/51 - Hepatitis viruses
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

The present invention relates to an automatic purification device for separating a target substance from a plurality of biological samples, and more specifically relates to an automatic biological sample purification device having a magnetic-field-applying unit for purifying the biological sample; the magnetic-field-applying unit being integrated with a heating part in such a way as to allow vertical movement. Also, the present invention relates to a method for extracting a target substance from a biological sample by using the automatic biological sample purification device having the magnetic-field-applying unit.

IPC Classes  ?

• C12M 1/18 - Multiple fields or compartments
• C12M 1/26 - Inoculator or sampler
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12N 15/00 - Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Abstract

Provided is porous films comprising carbon nanostructure-metal composite in which a coating film comprising carbon nanostructure-metal composite is formed on one surface or both surface of a membrane support having micro or nano pores and is subjected to hydrophilic surface modification. Since the metal size is several nm to several hundred nm, it is melted at a low temperature and is heat-treated at a low temperature to connect the metal with the carbon nano structure in a network structure. The metal is fused to the membrane support to manufacture a new type of composite porous films. When the new type of composite porous films are used as a water treatment membrane, it increases the flow rate of water to solve the problem of the membrane transmitting flow rate of the membrane.

IPC Classes  ?

• B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
• B01D 69/12 - Composite membranes; Ultra-thin membranes
• C08J 7/12 - Chemical modification
• C08J 7/18 - Chemical modification with polymerisable compounds using wave energy or particle radiation

Abstract

Provided is a portable gas chromatograph-mass spectrometer (GCMS) capable of quantitatively/qualitatively analyzing volatile organic compounds in the air up to an infinitesimal amount in real-time, more particularly, to the portable GCMS for monitoring volatile organic compounds in real time which integrally includes a Gas Chromatograph (GC) including a sampling device into which volatile organic compounds are injected, a small-sized gas chromatograph module, and a nitrogen supply source for supplying carrier gases of the gas chromatograph module; a Mass Spectrometer (MS) including a vacuum chamber and a quadrupole mass spectrometer included in the vacuum chamber; and a GCMS interface connecting the GC and the MS, as is constituted in one body. The portable GCMS is easy to use on the spot, minimizes the consumable parts, detects promptly in real-time on the spot.

IPC Classes  ?

• G01N 30/72 - Mass spectrometers
• G01N 30/02 - Column chromatography

Abstract

There is provided a sample preconcentrator. The sample preconcentrator in which a sample gas injection port is coupled to a dried gas supply source and a gas analysis system to concentrate a sample gas comprises a sample concentrating unit containing an absorbent that is composed of carbon nanotube-metal nanocomplexes; a conduit switching valve for selectively coupling the sample gas injection port to the dried gas supply source and the gas analysis system and controlling the absorption and desorption of the sample gas from the sample concentrating unit; and a plurality of conduits for connecting the sample gas injection port, the dried gas supply source, the gas analysis system, the sample concentrating unit and the conduit switching valve.

IPC Classes  ?

• G01N 1/40 - Concentrating samples
• G01N 1/22 - Devices for withdrawing samples in the gaseous state
• B82B 3/00 - Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units

Abstract

There is provided a high throughput oligonucleotide synthesizer used for the chemical synthesis of oligo DNA/RNA or protein in which a high throughput oligonucleotide synthesizer in which one or more synthetic reactor modules (plate) with hundreds or thousands of individual synthetic reactors are installed, a reagent valve for inputting a reagent required to synthesis into each of the individual synthetic reactors is mounted, the synthetic reactor modules are moved and arranged to match valve nozzles (inlets), and the reagents are divisionally provided to the individual synthetic reactors in sequence in accordance with sequence information (arrangement) which is inputted by a user to chemically synthesize oligo DNA/RNA or protein with a reactive microparticle media in the synthetic reactor module, in an automation synthesizer used for the chemical synthesis of oligo DNA/RNA or protein.

IPC Classes  ?

• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

Provided is a carbon nanotube-metal thermal conductive composite material. More particularly, provided is a novel type of thermal conductive composite material including a carbon nanotube- metal composite in combination with a metal. The thermal conductive composite material has improved impact strength and physical and mechanical properties, and thus is expected to contribute to industrial development in the field of bioengineering, particularly molecular diagnosis, when used in a thermal block of a thermal cycler.

IPC Classes  ?

• B82B 1/00 - Nanostructures formed by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
• C09K 5/00 - Heat-transfer, heat-exchange or heat-storage materials, e.g. refrigerants; Materials for the production of heat or cold by chemical reactions other than by combustion

Abstract

Disclosed are a fluorescent composition for staining DNA and a method for measuring DNA amplification using the same. More particularly, disclosed are a novel fluorescent compound that emits light by intercalating with DNA and a method for measuring DNA amplification using the same. The novel fluorescent compound is advantageous in that, due to high sensitivity, target-specific amplification products by real-time polymerase chain reaction(PCR) can be accurately quantified without affecting the PCR reaction. In addition, the compound is unharmful to the human body, can be prepared at low cost, and allows monitoring of various DNA and RNA reactions in vitro or in vivo.

IPC Classes  ?

• C09K 11/06 - Luminescent, e.g. electroluminescent, chemiluminescent, materials containing organic luminescent materials

Abstract

The present invention relates to a primer and a probe for detecting malaria plasmodium and a detection method using the same, and more particularly, to a primer and a probe for detecting genes of malaria plasmodium existing in a biological sample and an environmental sample, and to a method for detecting genes of malaria plasmodium through a polymerase chain reaction using the primer and the probe. The present invention detects malaria plasmodium in a quicker and more accurate manner as compared with conventional malaria plasmodium detection methods, and detects malaria plasmodium at one time in a real-time basis even in cases where Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale are mixed in a sample. In addition, a dried mixture for a polymerase chain reaction, containing the primer and the probe, can be stored while maintaining the performance of the mixture the same as that of the liquid state, and therefore, can be used for a detection kit.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

The present invention relates to a method for quickly detecting infectious microorganisms in an unknown sample, and more particularly to a method for detecting infectious microorganisms existing in an unknown sample in a short time by real-time polymerase chain reaction and cell culture methods. The detection method of the present invention can overcome the disadvantage of real-time polymerase chain reaction in which microorganisms are detected in a detection sample if nucleic acids exist in the microorganism even when the microorganisms are non-infectious. In addition, in contrast to conventional cell culture, the detection method of the present invention can selectively and quickly detect only infectious microorganisms. The detection method can detect infectious microorganisms in a water system as well as be widely used in food inspection such as testing of drinking water, restaurant sanitation inspection, agricultural, livestock, and fishery products inspections, and the like.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
• C12R 1/92 - Viruses
• C12R 1/90 - Protozoa

Abstract

The present invention relates to a compound for effectively inhibiting the activity of ribonuclease which promotes the decomposition of collected ribonucleic acid, and a container for storing a sample containing the same. The compound for inhibiting the activity of ribonuclease and the container for storing a sample of the present invention can be effectively used for the storage of ribonucleic acid during extraction or after extracted.

IPC Classes  ?

• C07D 403/12 - Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group containing two hetero rings linked by a chain containing hetero atoms as chain links
• C07D 403/08 - Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group containing two hetero rings linked by a carbon chain containing alicyclic rings
• C07D 403/02 - Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group containing two hetero rings

Abstract

The present invention relates to a primer specific to the genes of the new influenza A virus subtype H1N1 (the 2009 H1N1 influenza virus), to a probe and to a method for diagnosing the new influenza A virus subtype H1N1 using the primer and the probe. More particularly, the present invention relates to a primer for simultaneously diagnosing the genes of the new influenza A virus subtype H1N1 through a polymerase chain reaction, to a diagnosis kit comprising an internal control and the primer, and to a diagnosis method using the kit. The primer and the probe of the present invention can be produced into a dry kit which enables the new influenza A virus subtype H1N1 in a human body to be detected in a quick and accurate manner without causing a cross-reaction with a new influenza A virus subtype H1N1 existing in the human body, and which achieves an improvement in storage safety and which does not require any expertise for a tester when being used.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• G01N 33/569 - Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

The present invention relates to an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalentely bonding siRNA and a polymeric compound for improving the biostability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the biostability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relatively low concentration. Therefore, the conjugate can advantageously be used not only as an siRNA treatment tool for cancers and other infectious diseases, but also as a novel type siRNA delivery system.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

Provided is a carbon nanostructure-metal composite nanoporous film in which a carbon nanostructure-metal composite is coated on one surface or both surfaces of a membrane support having micro- or nano-sized pores. A method for manufacturing a carbon nanostructure-metal composite nanoporous film, includes: dispersing a carbon nanostructure-metal composite in a solvent at the presence of a surfactant and coating the carbon nanostructure-metal composite on one surface or both surfaces of a membrane support; and fusing the metal on the membrane support by heating the coated membrane support. The metal in carbon nanostructure-metal composite nanoporous film melts at a low temperature since a size of a metal of the carbon nanostructure-metal composite is several nm to several-hundred nm.

IPC Classes  ?

• B01D 69/00 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
• B01D 71/00 - Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor

Abstract

The present invention relates to an integrated real-time nucleic acid analysis apparatus and to a method using same. More particularly, the present invention relates to an integrated real-time nucleic acid analysis apparatus which performs a qualitative analysis and a quantitative analysis at the same time on genes corresponding to a plurality of biological samples of various types, and to a method for detecting a target nucleic acid using same. The integrated real-time nucleic acid analysis apparatus and the method for detecting a target nucleic acid using same according to the present invention perform just a single test on a variety of targets, requested for a variety of analytes, in a quick and accurate manner, and thus can be efficiently used in a hospital or the like where diagnoses for diseases are required.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12M 1/38 - Temperature-responsive control
• C12N 15/09 - Recombinant DNA-technology