Abstract

The present invention relates to a double-helix oligonucleotide construct comprising a double-stranded miRNA and a composition for preventing or treating cancer comprising the same. More particularly, the present invention relates to a double-helix oligonucleotide construct comprising miR-544a characterized by a method that effectively inhibits the proliferation of cancer cells or induces a voluntary death of cancer cells, and an anticancer composition comprising the construct.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61P 35/00 - Antineoplastic agents

Abstract

The invention relates to an adapter for mounting a conductive pipette, a sample tube opening/closing device, and an automatic sample analysis system and, more specifically, to an adapter for mounting a conductive pipette, a sample tube opening/closing device, and an automatic sample analysis system, in which dispensing of a liquid sample and extraction, amplification and testing of nucleic acids are integrally carried out. The sample tube opening/closing device includes: a housing that forms an inner space isolated from the outside and includes a door for carrying in/out a multi-well plate for biological samples, including a plurality of sample tubes in which biological samples are accommodated; and a sample tube opening/closing part that is installed in the inner space to be spaced apart from the multi-well plate for biological samples and automatically opens and closes the sample tubes.

IPC Classes  ?

• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• B01L 3/02 - Burettes; Pipettes

Abstract

The present invention relates to a method for isolating a nucleic acid by using: a binding buffer containing acetic acid or phosphoric acid; and the pH difference between binding and washing buffers, and an elution buffer, wherein silica magnetic particles are added to a biological sample including a nucleic acid to which a binding buffer containing acetic acid, phosphoric acid, etc., has been added, thereby binding the nucleic acid to the magnetic particles, after which a magnetic field is applied to separate the magnetic particles, and the nucleic acid is purified in a basic pH elution buffer. The method uses the pH difference, and can isolate the nucleic acid more quickly and efficiently than centrifugation or gravity separation. In addition, since ethanol is not used, high-purity nucleic acid can be isolated and purified to obtain more accurate results in subsequent experiments.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C07H 1/06 - Separation; Purification
• B03C 1/01 - Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
• B03C 1/28 - Magnetic plugs and dipsticks

Abstract

The present invention relates to a nucleic acid amplification test apparatus, and an automatic sample analysis system having same and, more particularly, to a nucleic acid amplification test apparatus in which separation and purification, dispensation, amplification, and testing of samples are performed integrally, and an automatic sample analysis system having same. Disclosed in the present invention is a nucleic acid amplification test apparatus comprising: a housing (30) having an inner space (S1) isolated from the outside; a multi-well plate insertion unit (100) into which a multi-well plate (40) having a plurality of reaction tubes (1) accommodating a target nucleic acid solution is inserted through an automatic purification and dispensing apparatus (10); a sealing plate insertion unit (200) into which a sealing plate (50) having a sealing means for sealing the inlets of the plurality of reaction tubes (1); a fluorescence detection unit (400) disposed on an upper side of the sealing plate insertion unit (200) and detecting a target nucleic acid in the reaction tubes (1); and a temperature control block (500) disposed on a lower side of the multi-well plate insertion unit (100) and controlling the temperature of the reaction tubes (1), wherein the reaction tubes (1) are moved relatively so as to be adjacent to the sealing means to be then sealed by the sealing means.

IPC Classes  ?

• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
• B01L 3/02 - Burettes; Pipettes
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

The present invention relates to a double-stranded oligonucleotide capable of inhibiting amphiregulin expression in a very specific and highly efficient manner, preferably a double-stranded oligonucleotide comprising a sequence in the form of an RNA/RNA, DNA/DNA or DNA/RNA hybrid, and the use of a double-stranded oligonucleotide structure, which comprises the double-stranded oligonucleotide, and nanoparticles, for preventing or treating obesity.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61K 47/60 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
• A61P 3/04 - Anorexiants; Antiobesity agents

Abstract

The present invention relates to a double-stranded oligonucleotide which can highly specifically and efficiently inhibit an amphiregulin expression and, preferably, a double-stranded oligonucleotide comprising a sequence in the form of RNA/RNA, DNA/DNA or DNA/RNA hybrid, a double-stranded oligonucleotide structure comprising the double-stranded oligonucleotide, nanoparticles comprising the double-stranded oligonucleotide structure, and a fibrosis or respiratory disease preventive or therapeutic use thereof.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61P 11/00 - Drugs for disorders of the respiratory system
• A61K 9/19 - Particulate form, e.g. powders lyophilised
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides

Abstract

The present invention relates to a double-stranded oligonucleotide which can highly specifically and efficiently inhibit an amphiregulin expression and, preferably, a double-stranded oligonucleotide comprising a sequence in the form of RNA/RNA, DNA/DNA or DNA/RNA hybrid, a double-stranded oligonucleotide structure comprising the double-stranded oligonucleotide, nanoparticles comprising the double-stranded oligonucleotide structure, and a fibrosis or respiratory disease preventive or therapeutic use thereof.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61P 11/00 - Drugs for disorders of the respiratory system
• A61K 9/19 - Particulate form, e.g. powders lyophilised
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides

Abstract

The present invention relates to: a double-stranded oligonucleotide which can highly specifically and efficiently inhibit the proliferation of Severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2), preferably a double-stranded oligonucleotide comprising a sequence in the form of RNA/RNA, DNA/DNA or a DNA/RNA hybrid; a double-stranded oligonucleotide structure and nanoparticles comprising the double-stranded oligonucleotide; and a use thereof for treating COVID-19.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 9/51 - Nanocapsules
• A61P 31/14 - Antivirals for RNA viruses
• A61P 11/00 - Drugs for disorders of the respiratory system
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides

Abstract

The present disclosure relates to a trackable precise-sample-injection device for a multi-well plate and, more specifically, to a trackable precise-sample-injection device for a multi-well plate, the device being capable of: preventing cross-contamination that can occur when many samples are manually put into a multi-well plate, and a diagnosis error caused by the injection of a sample into the wrong well or repeated injection; and simply recording and tracking information about wells into which samples are injected. The present disclosure relates to a trackable precise-sample-injection device for a multi-well plate, the device being capable of being efficiently used for a pooling inspection, and the like by injecting a plurality of samples into a multi-well plate.

IPC Classes  ?

• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

The present invention relates to a composition using a urine sample for diagnosis of a kidney disease and, more specifically, to a urine test composition and a kit for diagnosis of a kidney disease, each comprising an agent specifically detecting amphiregulin from a urine sample, and a method for diagnosing a kidney disease by using the composition. According to the present invention, not only is there an advantage of being able to conveniently diagnose a kidney disease in a non-invasive manner using a urine sample for a kidney disease to which early diagnosis is important, but also the application of the present invention to a patient diagnosed with chronic kidney disease can advantageously predict the plausibility of progression into end-stage renal disease in advance.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
• G01N 33/53 - Immunoassay; Biospecific binding assay; Materials therefor

Abstract

The present invention relates to a double-stranded oligonucleotide capable of inhibiting CTGF expression with a very specific and high efficiency, a double-stranded oligonucleotide structure and nanoparticles comprising the double-stranded oligonucleotide, and a use thereof in preventing or treating of fibrotic or respiratory diseases.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides

Abstract

A conductive paste composition according to the present disclosure contains silver-coated copper nanowires with a core-shell structure; a binder mixture containing a silicone resin binder and a hydrocarbon-based resin binder; and an organic solvent, such that the conductive paste composition has a low sheet resistance and may withstand a high temperature, thereby implementing excellent conductivity and electromagnetic wave shielding properties. Furthermore, the conductive paste may be widely used in various fields such as electromagnetic wave shielding, solar cell electrodes, electronic circuits.

IPC Classes  ?

• C08K 3/08 - Metals
• C08J 7/044 - Forming conductive coatings; Forming coatings having anti-static properties
• C08J 5/18 - Manufacture of films or sheets
• H01B 1/22 - Conductive material dispersed in non-conductive organic material the conductive material comprising metals or alloys
• B82Y 40/00 - Manufacture or treatment of nanostructures
• B82Y 30/00 - Nanotechnology for materials or surface science, e.g. nanocomposites
• C08K 9/02 - Ingredients treated with inorganic substances
• C08K 7/06 - Elements

Abstract

Provided is an electric grill capable of precisely controlling the temperature of a grilling plate by directly applying a heating paste including carbon nanotubes to the bottom of the grilling plate. The present invention includes the grilling plate on which food to be cooked is placed; a heating body that is applied so as to be arranged on the lower portion of the grilling plate so as to heat the grilling plate; a heat insulating material which is disposed to be spaced apart from the heating body at a predetermined distance so as to block heat conduction to the outside; and a temperature sensor which is disposed between the heating body and the heat insulating material so as to measure the temperature of an upper plate. A pair of electrodes for supplying electricity to the heating body are arranged along opposite edges of both sides of the heating body, and a heating region can be divided and controlled by dividing and combining the pair of electrodes, the heating body, the coating thickness, and the like.

IPC Classes  ?

• A47J 37/06 - Roasters; Grills; Sandwich grills
• H05B 3/26 - Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater non-flexible heating conductor mounted on insulating base
• H05B 3/03 - Electrodes
• H05B 1/02 - Automatic switching arrangements specially adapted to heating apparatus
• F24C 7/08 - Arrangement or mounting of control or safety devices
• A47J 36/04 - Selection of specific materials, e.g. heavy bottoms with copper inlay or with insulating inlay the materials being non-metallic

Abstract

The present invention relates to a system structure capable of performing real-time detection of nucleic acid extraction and amplification reactions and amplified results in a device for implementing a polymerase chain reaction (PCR). A PCR system includes a nucleic acid extraction cartridge configured to extract a nucleic acid of a biological sample via a nucleic acid extraction reagent stored therein and form a PCR preliminary mixture by being additionally mixed with a polymerase reaction dried product, a PCR plate inserted into the nucleic acid extraction cartridge, having a channel coupled to the nucleic acid extraction cartridge, and receives a nucleic acid solution or the PCR preliminary mixture extracted from the nucleic acid extraction cartridge and diversely accommodates a PCR reaction dried product containing a dried primer/probe or a primer/probe in at least one reaction well, a temperature control module disposed above the PCR plate 200 and including a pair of heating blocks 310 and 320 adjacent to the reaction well (W) to apply different temperatures, and a scanning module scanning a concentration of a reactant amplified in the reaction well (W).

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Disclosed are a double stranded oligonucleotide construct, configured such that a hydrophilic material and a hydrophobic material are conjugated through a simple covalent bond or a linker-mediated covalent bond to both ends of a double stranded oligonucleotide in order to efficiently deliver an androgen-receptor-specific oligonucleotide into a cell, a nanoparticle capable of being produced by self-assembling double stranded oligonucleotide constructs in an aqueous solution through hydrophobic interactions, and a composition for preventing hair loss or promoting hair growth containing the double stranded oligonucleotide construct. The double stranded oligonucleotide construct including the androgen-receptor-specific oligonucleotide and the composition for preventing hair loss or promoting hair growth containing the same as an active ingredient can suppress the expression of an androgen receptor with high efficiency without side effects, and can thus exhibit excellent effects on preventing hair loss, particularly androgenetic alopecia, alopecia areata, and telogen effluvium, and promoting hair growth.

IPC Classes  ?

• A61K 47/26 - Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• A61K 47/60 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• A61K 47/58 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
• A61K 47/59 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61Q 7/00 - Preparations for affecting hair growth
• A61K 8/60 - Sugars; Derivatives thereof
• A61K 8/86 - Polyethers
• A61K 8/88 - Polyamides
• A61K 8/84 - Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions other than those involving only carbon-to-carbon unsaturated bonds
• A61K 8/81 - Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
• A61P 17/14 - Drugs for dermatological disorders for baldness or alopecia

Abstract

The present invention pertains to: a double-stranded oligonucleotide construct having a structure in which a hydrophilic substance and a hydrophobic substance are conjugated by a simple covalent bond or a linker-mediated covalent bond at both ends of a DKK1-specific double-stranded oligonucleotide to efficiently deliver the double-stranded oligonucleotide into cells; a nanoparticle capable of being produced through self-assembly of the double-stranded oligonucleotide construct through a hydrophobic interaction in an aqueous solution; and a hair-loss-preventing and hair-growth-promoting composition containing the double-stranded oligonucleotide construct or the nanoparticle. A double-stranded oligonucleotide construct including a DKK1-specific double-stranded oligonucleotide, a nanoparticle, and a hair loss prevention or hair growth composition containing the double-stranded oligonucleotide construct or the nanoparticle as an active ingredient according to the present invention very efficiently suppress the expression of DKK1 without side effects and are remarkably effective for preventing hair loss and promoting hair growth, and can thus be very usefully used for a composition for preventing hair loss and promoting hair growth.

IPC Classes  ?

• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61P 17/14 - Drugs for dermatological disorders for baldness or alopecia

Abstract

A heater integrated gas chromatography (GC) column device according to the present invention is capable of precisely and uniformly controlling a temperature by having a high thermal conductivity, and raising and lowering a temperature at a high speed by having a low thermal mass, such that a measuring time is significantly decreased. The GC column is in contact with a bobbin with a homogeneous temperature distribution, and thus a temperature is homogeneously distributed in each GC column. Further, the heater integrated GC column device according to the present invention has the above-described effects and may have a smaller size.

IPC Classes  ?

• G01N 30/30 - Control of physical parameters of the fluid carrier of temperature
• G01N 30/60 - Construction of the column
• H05B 3/14 - Heating elements characterised by the composition or nature of the materials or by the arrangement of the conductor characterised by the composition or nature of the conductive material the material being non-metallic
• G01N 30/02 - Column chromatography

Abstract

A sample concentrator tube having a heat-resistant planar heating element adhered thereto, an analysis device comprising the same, and an analysis method using the same, according to the present invention, have an effect capable of precisely controlling the temperature by uniformly and rapidly heating the sample concentrator tube to a target temperature for desorption, and capable of almost simultaneously desorbing an adsorbed sample in any part of an adsorbent by minimizing a local temperature difference of the adsorbent in the tube. In addition, it is possible to minimize chemical noise by preventing thermal denaturation of the adsorbent caused by over-heating, and there is an advantage of excellent reproducibility as well as an effect of being inexpensive, economical, and excellent in energy efficiency.

IPC Classes  ?

• G01N 1/40 - Concentrating samples
• G01N 1/44 - Sample treatment involving radiation, e.g. heat
• G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
• H05B 3/26 - Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater non-flexible heating conductor mounted on insulating base
• H05B 3/46 - Heating elements having the shape of rods or tubes non-flexible heating conductor mounted on insulating base
• H05B 3/06 - Heater elements structurally combined with coupling elements or with holders
• B01D 53/04 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography with stationary adsorbents

Abstract

The present invention relates to a probe having octamine or octamine derivative bound thereto in addition to a reporter and a quencher to thus allow the quencher to more effectively suppress light emitted by the reporter, and to uses of the probe. When the probe according to the present invention is utilized, octamine or octamine derivative bound to the probe effectively suppresses the light emitted by the quencher for the reporter, and results in effects such as i) a reduction in base fluorescence, ii) an increase in delta fluorescence, and iii) a decrease in the value of cycle at threshold, thus allowing the probe to be effectively used in a variety of real-time polymerase chain reactions requiring accuracy and sensitivity.

IPC Classes  ?

• C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

The present invention relates to an extraction apparatus capable of simultaneously extracting target materials from multiple biological samples and, more particularly, to a target material extraction apparatus in which magnetic bar blocks can be replaced according to kinds of multi-well plates to be inserted into a cartridge. When used, the target material extraction apparatus of the present invention is operated in such a manner that among various multi-well plates having different numbers of wells, multi-well plates suitable for a use purpose are loaded into a cartridge within the apparatus and magnetic bar blocks equipped with magnetic bars suitable therefor are selected and loaded. Thus, the apparatus has the advantage of selectively applying various multi-well plates to one installment according to the number of samples from which a target material is extracted or to the amount of the target material to be extracted.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

The present invention relates to a lactic acid bacterium isolated from human mother's milk, more precisely a Lactobacillus gasseri BNR17 strain that is isolated from Korean mother's milk and has excellent probiotic activity including acid resistance, bile acid resistance and antimicrobial activity and weight gaining inhibitory effect as well. Again, the Lactobacillus gasseri BNR17 of the present invention has excellent acid resistance, bile acid resistance, enteric absorption activity and antimicrobial activity against pathogenic microorganisms, in addition to the weight gaining inhibitory effect by synthesizing indigestible polysaccharides from monosaccharides included in food taken and releasing the synthesized polysaccharides out of the body. Therefore, the strain of the invention, owing to such beneficiary effects, can be effectively used not only for the production of fermented milk, other fermented food products and animal feeds but also for the production of live cell products and food additives for preventing weight gaining.

IPC Classes  ?

• C12R 1/225 - Lactobacillus
• A23L 33/18 - Peptides; Protein hydrolysates
• A23L 33/135 - Bacteria or derivatives thereof, e.g. probiotics
• A23C 9/123 - Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
• C07K 14/335 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Lactobacillus (G)
• C12N 1/20 - Bacteria; Culture media therefor
• A61K 38/00 - Medicinal preparations containing peptides

Abstract

An electric roasting pan according to the present invention comprises: a roasting pan; a heating layer being in surface contact with the roasting pan and comprising a carbon nanotube and a silicone-based adhesive; and an electrode in contact with the heating layer. The electric roasting pan has excellent thermal efficiency thanks to the minimal heat loss thereof, can reach a target temperature within a short time to reduce a preheating time, and affords excellent cooking quality. Furthermore, the electric roasting pan can substantially prevent the occurrence of temperature deviation across the area of the roasting pan during a temperature increase and is of high durability and safety.

IPC Classes  ?

• H05B 3/14 - Heating elements characterised by the composition or nature of the materials or by the arrangement of the conductor characterised by the composition or nature of the conductive material the material being non-metallic
• H05B 1/02 - Automatic switching arrangements specially adapted to heating apparatus
• A47J 36/04 - Selection of specific materials, e.g. heavy bottoms with copper inlay or with insulating inlay the materials being non-metallic
• A47J 37/10 - Frying pans, e.g. frying pans with integrated lids or basting devices
• C09D 101/28 - Alkyl ethers

Abstract

The present invention relates to the structure of an analysis plate applied to a high-speed polymerase chain reaction (PCR), and to a PCR analysis plate used for implementing an analysis of a real-time PCR, a real-time nested PCR and a post-PCR lateral flow hybridization reaction. The present invention is provided with: a check valve for enabling the maintaining of positive pressure when an elastic film expands into a convex form by having a solution pushed therein by the positive pressure; a lateral flow analysis module for analyzing a post-PCR follow-up PCR or lateral flow; and a shut-off valve enabling the controlling of the movement of the solution after each reaction ends. A high-speed PCR analysis plate may be provided whereby, by pressing, by means of a temperature-controllable heating block, the elastic film, which is in a convex form by the solution, of a PCR unit, a PCR solution may undergo rapid temperature circulation with minimum heat resistance, and a PCR dried material and a nucleic acid solution may be homogenized and mixed.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

The present invention relates to a double-helix oligonucleotide construct comprising a double-stranded miRNA and a composition for preventing or treating cancer comprising the same. More particularly, the present invention relates to a double-helix oligonucleotide construct comprising miR-544a characterized by a method that effectively inhibits the proliferation of cancer cells or induces a voluntary death of cancer cells, and an anticancer composition comprising the construct.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61P 35/00 - Antineoplastic agents

Abstract

A bio sample collection device including: a housing; a cap part detachably fastened to the housing, and including a bio sample collector for collecting a bio sample; and a bio sample processing liquid storage unit storing bio sample processing liquid, the bio sample processing liquid storage unit being formed to be broken when the cap part is combined with the housing after bio sample is collected so that the bio sample processing liquid is mixed with the bio sample.

IPC Classes  ?

• G01N 1/02 - Devices for withdrawing samples
• A61B 10/02 - Instruments for taking cell samples or for biopsy
• C12M 1/26 - Inoculator or sampler

Abstract

a and miR-8078, and characterized by a method for effectively inhibiting cancer cell proliferation or inducing cancer cell apoptosis; and a pharmaceutically acceptable carrier.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61P 35/00 - Antineoplastic agents
• A61K 31/25 - Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids with polyoxyalkylated alcohols, e.g. esters of polyethylene glycol
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides

Abstract

The present invention relates to an epoxy paste composition including silver-coated copper nanowires having a core-shell structure, and a conductive film including the same.

IPC Classes  ?

• C09D 5/24 - Electrically-conducting paints
• H01B 1/22 - Conductive material dispersed in non-conductive organic material the conductive material comprising metals or alloys
• B32B 5/16 - Layered products characterised by the non-homogeneity or physical structure of a layer characterised by features of a layer formed of particles, e.g. chips, chopped fibres, powder
• H01B 1/02 - Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of metals or alloys
• C08K 9/02 - Ingredients treated with inorganic substances
• C08K 3/08 - Metals
• C09D 163/00 - Coating compositions based on epoxy resins; Coating compositions based on derivatives of epoxy resins
• H05K 9/00 - Screening of apparatus or components against electric or magnetic fields

Abstract

The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.

IPC Classes  ?

• H01F 1/06 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• H01F 1/00 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
• C01B 33/18 - Preparation of finely divided silica neither in sol nor in gel form; After-treatment thereof

Abstract

A sample plate for MALDI mass spectrometry, according to the present invention, enables separately positioning, by means of a plastic insulation plate, metal wiring and metal dots onto which an analyte sample is to be loaded, and electrically connecting same by means of a via or a metal portion, and thus the energy transferred into the plate when radiating a laser beam on the target (metal dots) may be reduced compared to a sample plate using a base metal, and thus laser energy may be concentrated on the target, and an effect may be achieved whereby heat loss is minimized.

IPC Classes  ?

• H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
• G01N 27/62 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electric discharges, e.g. emission of cathode
• H01J 49/34 - Dynamic spectrometers

Abstract

The present invention relates to a matrix-assisted laser desorption ionization mass spectrometry method and, specifically, a mass spectrometry method according to the present invention comprises the steps of: acquiring a mass spectrum of an analyte by performing matrix-assisted laser desorption ionization of the analyte, wherein a detection spectrum, which is the mass spectrum of the analyte, is acquired using each of two or more matrixes different from one another; and removing, from each detection spectrum, a peak of a corresponding matrix to obtain a matrix-removed spectrum, and then acquiring a corrected mass spectrum of the analyte on the basis of a matrix-removed spectrum for each of different matrixes.

IPC Classes  ?

• H01J 49/16 - Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
• H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
• H01J 49/40 - Time-of-flight spectrometers
• H01J 49/10 - Ion sources; Ion guns

Abstract

A biological sample processing apparatus, including: a pipette block with which a plurality of pipettes for sucking or discharging a biological sample in a multi-well plate in which wells are arranged in a matrix shape along row and column directions are detachably coupled; a pipette block forward and backward transfer unit configured to move the pipette block along a forward and backward direction along a process direction; a pipette block top and bottom transfer unit configured to move the pipette block along a vertical direction; a magnetic field applying unit disposed below the multi-well plate for applying a magnetic field to a well of the multi-well plate; and a heating unit disposed below the multi-well plate so as to be spaced apart from the magnetic field applying unit, for heating a well of the multi-well plate.

IPC Classes  ?

• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
• B01L 3/02 - Burettes; Pipettes

Abstract

The present invention relates to a pharmaceutical composition for treatment of cancer, which comprises, as an active ingredient, one or more miRNAs selected from the group consisting of miR-3670, miR-8078, and miR-4477a. The pharmaceutical composition for treatment of cancer according to the present invention exhibits excellent effects of inhibiting cancer cell proliferation and inducing cancer cell apoptosis. Thus, the pharmaceutical composition of the present invention can be effectively used as an anticancer therapeutic agent.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61P 35/00 - Antineoplastic agents

Abstract

The present invention relates to a lactic acid bacterium isolated from human mother's milk, more precisely a Lactobacillus gasseri BNR17 strain that is isolated from Korean mother's milk and has excellent probiotic activity including acid resistance, bile acid resistance and antimicrobial activity and weight gaining inhibitory effect as well. Again, the Lactobacillus gasseri BNR17 of the present invention has excellent acid resistance, bile acid resistance, enteric absorption activity and antimicrobial activity against pathogenic microorganisms, in addition to the weight gaining inhibitory effect by synthesizing indigestible polysaccharides from monosaccharides included in food taken and releasing the synthesized polysaccharides out of the body. Therefore, the strain of the invention, owing to such beneficiary effects, can be effectively used not only for the production of fermented milk, other fermented food products and animal feeds but also for the production of live cell products and food additives for preventing weight gaining.

IPC Classes  ?

• A61K 35/74 - Bacteria
• C12N 1/20 - Bacteria; Culture media therefor
• C12R 1/225 - Lactobacillus
• A23L 33/18 - Peptides; Protein hydrolysates
• A23L 33/135 - Bacteria or derivatives thereof, e.g. probiotics
• A23C 9/123 - Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
• C07K 14/335 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Lactobacillus (G)
• A61K 38/00 - Medicinal preparations containing peptides

Abstract

The present invention relates to an oligonucleotide structure and a method for preparing the same and, more particularly, to an oligonucleotide structure in which a polymer compound is linked to an oligonucleotide via a covalent bond to improve in vivo stability of the oligonucleotide and cellular delivery efficiency of the oligonucleotide; and to a method for preparing the same. The oligonucleotide structure is improved into a homogenous material, thereby solving the problem in material verification due to polydispersion characteristics occurring when a hydrophilic material linked to the oligonucleotide is a synthetic polymer; the oligonucleotide structure is easy to synthesize compared with the existing process; and the size of a double-stranded oligo RNA structure can be accurately adjusted through the control of the repetition number of a hydrophilic material block, and thus, the gene expression regulation function of the oligonucleotide does not deteriorate through the synthesis of the optimized oligonucleotide structure, and the oligonucleotide can be delivered into cells at even a relatively low-concentration dosage. Therefore, the oligonucleotide structure of the present invention can be useful as a novel type oligonucleotide delivery system as well as a tool for treating cancers, infectious diseases, and the like.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• A61K 9/51 - Nanocapsules
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• A61K 47/48 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives the non-active ingredient being chemically bound to the active ingredient, e.g. polymer drug conjugates
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides

Abstract

The present invention relates to a novel siRNA, and a high-efficiency double-stranded oligo RNA structure containing the same, and a nanoparticle containing the high-efficiency double-stranded oligo RNA structure. The double-stranded oligo RNA structure has a structure in which a hydrophilic material and a hydrophobic material are conjugated to both ends of a double-stranded oligo RNA (siRNA) via a simple covalent bond or linker-mediated covalent bond in order to be efficiently delivered into cells, and may be converted into a nanoparticle form in an aqueous solution by hydrophobic interactions of double-stranded oligo RNA structures. It is preferable that the siRNA contained in the double-stranded oligo RNA structure is an siRNA specific for fibrosis or respiratory disease-related gene, particularly, amphiregulin or stratifin. In addition, the present invention relates to a pharmaceutical composition for preventing or treating fibrosis or respiratory diseases, containing an siRNA, a high-efficiency double-stranded oligo RNA structure containing the siRNA, or a nanoparticle containing the high-efficiency double-stranded oligo RNA structure, as an active ingredient. In addition, the present invention relates to a method of preventing or treating fibrosis or respiratory diseases, including administering the pharmaceutical composition for preventing or treating fibrosis or respiratory diseases to a subject in need thereof.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides

Abstract

The present invention provides a novel azo compound having a quenching ability for the material that exhibits a luminescent phenomenon at an excited energy level, a quencher comprising the novel azo compound, a use of the quencher and a method for preparing the azo compound. The quencher according to the present invention may exhibit excellent characteristics in a wavelength absorption region.

IPC Classes  ?

• C09B 31/14 - Heterocyclic components
• C07D 213/72 - Nitrogen atoms
• C07D 277/82 - Nitrogen atoms
• C09B 31/153 - Heterocyclic components containing a six-membered ring with one nitrogen atom as the only ring hetero atom
• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

The present invention relates to a magnetic particle separating device, and a method of separating and purifying nucleic acid or protein using the same. The device comprises: induction magnets (100); an induction magnet fixing part (200) having induction magnet fixing holes (210) for fixing the induction magnets (100); and a body (300) in which entry holes (310) are formed into which tubes (T) are inserted. Thus, the application and removal of a magnetic field to and from the body is made very convenient, so that the device can be very advantageously used in the separation and purification of nucleic acid or protein.

IPC Classes  ?

• B03C 1/01 - Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 1/40 - Concentrating samples
• B03C 1/28 - Magnetic plugs and dipsticks
• B03C 1/033 - Component parts; Auxiliary operations characterised by the magnetic circuit

Abstract

The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.

IPC Classes  ?

• G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• H01F 1/00 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
• H01F 1/06 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder
• C01B 33/18 - Preparation of finely divided silica neither in sol nor in gel form; After-treatment thereof

Abstract

The present invention relates to a micro chamber plate, and more particularly, to a micro chamber plate having micro chamber holes formed using a flowable film. Thus, samples can be injected into the micro chamber holes in a smoother manner compared to when samples are injected using a vacuum and/or centrifugal force. In addition, bubbles and excess samples in the micro chamber holes can be efficiently discharged, making it possible to perform reaction and analysis in a more accurate and efficient manner.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• C12Q 1/686 - Polymerase chain reaction [PCR]
• G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

A ceramic paste composition including carbon nanotubes or a carbon nanotube-metal composite and a silicone adhesive, wherein the silicone adhesive includes 0.1 to 10 wt % of a silanol group, and has a mole ratio of a phenyl group to a methyl group of 0.3 to 2.5. The ceramic paste composition has low sheet resistance, through which an excellent heat generating property, and shielding, absorbing and conducting properties may be implemented in one or more embodiments. Further, though the ceramic paste composition has a very high heat generating temperature of 400° C., as compared with general paste based on carbon nanotubes, the physical properties thereof may be maintained stably. In addition, the ceramic paste may be widely used in various fields including heat generating products such as those for keeping warmth or heating, and products for electromagnetic wave shielding and absorption, electrodes, electronic circuits, antennas, and the like.

IPC Classes  ?

• H01B 1/24 - Conductive material dispersed in non-conductive organic material the conductive material comprising carbon-silicon compounds, carbon, or silicon
• C04B 35/52 - Shaped ceramic products characterised by their composition; Ceramic compositions; Processing powders of inorganic compounds preparatory to the manufacturing of ceramic products based on non-oxides based on carbon, e.g. graphite
• H01B 1/04 - Conductors or conductive bodies characterised by the conductive materials; Selection of materials as conductors mainly consisting of carbon-silicon compounds, carbon, or silicon
• H01B 1/18 - Conductive material dispersed in non-conductive inorganic material the conductive material comprising carbon-silicon compounds, carbon, or silicon

Abstract

The present invention relates to a dengue virus-specific siRNA, a double-stranded oligo RNA structure comprising the siRNA, and a composition for inhibiting dengue virus replication, which comprises the same, in which the double-stranded oligo RNA structure comprises a hydrophilic compound and hydrophobic compound conjugated to both ends of the double-stranded RNA (siRNA) by a single covalent bond or a linker-mediated covalent bond so that they will be efficiently delivered into cells, and can be converted into nanoparticles by hydrophobic interactions between the double-stranded oligo RNA structures in an aqueous solution. The siRNA included in the double-stranded oligo RNA structure acts specifically on all dengue virus serotypes. The present invention also relates to a method for preparing the double-stranded oligo RNA structure, and a pharmaceutical composition for preventing or treating dengue virus infection, which comprises the double-stranded oligo RNA structure.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides

Abstract

A method of manufacturing a micro-chamber plate with a built-in sample, including: settling a micro-chamber plate for sample injection at a micro-chamber plate receiving part formed with an upper opening; disposing a cover for micro-chamber plate receiving part to cover the upper opening, the cover for micro-chamber plate receiving part having a provisional storing part and an auxiliary covering part connected with the provisional storing part and formed with a through-hole for auxiliary covering part; and manufacturing a micro-chamber plate with a built-in sample by putting the micro-chamber plate receiving part, on which the cover is disposed, into a centrifugal separator which can apply vacuum, applying centrifugal force and injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate through a vessel communication part which is formed at the provisional storing part to be communicated with the micro-chamber plate receiving part.

IPC Classes  ?

• B01L 1/00 - Enclosures; Chambers
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• G01N 21/64 - Fluorescence; Phosphorescence
• C12M 1/32 - Inoculator or sampler multiple field or continuous type

Abstract

An automated cell-free protein production system comprises: a protein expression reaction unit comprising a reaction vessel that includes a plurality of dialysis tubes, each including a dialysis membrane and being open at its top; a reaction temperature control unit configured to heat or cool the reaction vessel; a pipette array comprising a plurality of pipettes and configured to suck or discharge solutions using the pipettes; a pipette array moving unit configured to move the pipette array in an upward and downward direction, a forward and backward direction or a left and right direction so as to move solutions; a protein purification unit including a magnetic field application device; and a multi-well plate mounting unit having mounted therein a multi-well plate kit configured to supply solutions that are used for protein production.

IPC Classes  ?

• C07K 1/14 - Extraction; Separation; Purification
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/24 - Apparatus for enzymology or microbiology tube or bottle type
• C12M 1/32 - Inoculator or sampler multiple field or continuous type
• C12M 1/26 - Inoculator or sampler
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12P 21/00 - Preparation of peptides or proteins
• C07K 1/02 - General processes for the preparation of peptides in solution
• C07K 1/04 - General processes for the preparation of peptides on carriers
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• C07K 1/36 - Extraction; Separation; Purification by a combination of two or more processes of different types

Abstract

Provided are a SAMiRNA-magnetic nanoparticle complex capable of effectively delivering a double-stranded oligo RNA and magnetic nanoparticles into a cell and a composition capable of simultaneously performing diagnosis and therapy of diseases such as cancer, and the like, containing the same. More specifically, provided is the SAMiRNA-magnetic nanoparticle complex consisting of double-stranded oligo RNA-polymer structures in which a hydrophilic material and a second hydrophobic material are bound to the double-stranded oligo RNA by a simple covalent bond or a linker-mediated covalent bond, and the magnetic nanoparticles in which a first hydrophobic material is bound onto a surface of the magnetic material, as a core. The SAMiRNA-magnetic nanoparticle complex may have a homogeneous size by a hydrophobic interaction between the first hydrophobic material of the present invention and the second hydrophobic material of the double-stranded oligo RNA structure. In addition, the hydrophilic material and the second hydrophobic material bound to the double-stranded oligo RNA structure may improve in vivo stability of the double-stranded oligo RNA, an additionally bound ligand may deliver the SAMiRNA-magnetic nanoparticle complex into a target cell even at a relative low concentration of dosage, and the magnetic materials of the magnetic nanoparticles may be used as an imaging agent for diagnosis.

IPC Classes  ?

• A61K 47/48 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives the non-active ingredient being chemically bound to the active ingredient, e.g. polymer drug conjugates
• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• A61K 49/18 - Nuclear magnetic resonance (NMR) contrast preparations; Magnetic resonance imaging (MRI) contrast preparations characterised by a special physical form, e.g. emulsions, microcapsules, liposomes
• A61K 9/14 - Particulate form, e.g. powders
• A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
• A61K 9/51 - Nanocapsules
• A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
• A61K 33/24 - Heavy metals; Compounds thereof
• A61K 9/50 - Microcapsules

Abstract

2, four kinds of dNTPs, and reverse transcription polymerase in a single reaction tube. The composition for hot-start reverse transcription reaction is obtained by freezing or drying the composition. The composition show increased stability and long-term storage stability. Also, disclosed is a composition that additionally includes DNA polymerase, and, thus, enables a hot-start reverse transcription reaction and a PCR reaction to be sequentially performed. A method for amplifying a nucleic acid by using the composition. The composition of the invention can be conveniently and effectively used in multiplex reverse transcription PCRs or real-time quantitative reverse transcription PCR.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
• C12Q 1/42 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

Provided are an apparatus and a method for automatically analyzing biological samples capable of performing the entire processes of dissolving the biological samples in protease and cell lysate to dissolve a nucleic acid in a solution, attaching the nucleic acid to magnetic particles, finally washing the magnetic particle to which the nucleic acid is attached with an organic solvent, drying the magnetic particles using a vacuum pump, eluting the target nucleic acid attached to the magnetic particles in an aqueous solution, adding and mixing the eluted target nucleic acid into a vessel containing a nucleic acid amplification reagent, real-time detecting amplification by irradiating excitation light to a reactor simultaneously with regulating a temperature to perform amplification to measure fluorescence, inactivating an amplified product using an ultraviolet lamp after amplification, obtaining an image through electrophoresis, and analyzing a molecular weight in a single apparatus.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

Provided are a double-stranded oligo RNA structure and a method of preparing the same, and more specifically, a double-stranded oligo RNA structure in which a polymer compound is covalently bound to a double-stranded oligo RNA in order to improve stability in vivo and a cell delivery efficiency of the double-stranded oligo RNA, and a method of preparing the same. The double-stranded oligo RNA structure having the optimized structure according to the present invention may not inhibit functions of the double-stranded oligo RNA, but effectively improve stability and cell membrane permeability of the double-stranded oligo RNA, such that the double-stranded oligo RNA may be delivered into the cell even at a low concentration dosage thereof to be significantly used as a tool for treatment of cancer, infectious diseases, and the like, as well as a new delivery system of the double-stranded oligo RNA.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• A61K 9/14 - Particulate form, e.g. powders
• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides

Abstract

Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system.

IPC Classes  ?

• A61K 47/00 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
• A61K 9/107 - Emulsions
• A61K 47/48 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives the non-active ingredient being chemically bound to the active ingredient, e.g. polymer drug conjugates
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C08G 65/335 - Polymers modified by chemical after-treatment with organic compounds containing phosphorus
• C07F 7/08 - Compounds having one or more C—Si linkages
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• B82Y 5/00 - Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• A61K 47/34 - Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers

Abstract

Provided is a method of protein synthesis. The method of protein synthesis according to the present invention uses an automatic biological material purification apparatus including: a well plate kit; a heating part; and a magnetic field applying part, such that a plurality of target proteins may be more quickly and simply obtained as compared to target proteins obtained by using the existing method for expressing/purifying proteins through conventional cell culture, and a reproducible synthesis efficiency on the same proteins may be obtained due to no deviation between reaction wells.

IPC Classes  ?

• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• C07K 1/00 - General processes for the preparation of peptides
• C07K 1/14 - Extraction; Separation; Purification
• C12P 21/00 - Preparation of peptides or proteins
• B01J 19/00 - Chemical, physical or physico-chemical processes in general; Their relevant apparatus
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

A device for effectively removing organic compounds from air by a carbon nanotube-catalyst composite having the two functions of an adsorption/catalytic incineration agent is provided. The carbon nanotube-catalyst composite simultaneously adsorbs the organic compounds and completely decomposes them by a catalytic reaction, and the optimal reaction active temperature by catalytic incineration is low. The carbon nanotube-catalyst composite has a large surface area and has high adsorption performance and catalytic decomposition activity, and is thus applicable to filters that use the methods of adsorption and/or catalytic incineration. The device for removing organic compounds from air includes an adsorption/catalytic incineration reactor including the carbon nanotube-catalyst composite to remove organic compounds from air.

IPC Classes  ?

• F23G 5/40 - Portable or mobile apparatus
• B01D 53/02 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography
• B01D 53/86 - Catalytic processes

Abstract

th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 1/20 - Bacteria; Culture media therefor
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

Disclosed is an automatic nucleic acid purification apparatus which can prevent pollution due to aerosol generated from a biological sample containing high concentration target nucleic acid when the biological sample containing the high concentration target nucleic acid is mixed with other biological sample containing low concentration target nucleic acid or not containing the target nucleic acid. Further, disclosed is an automatic nucleic acid purification apparatus which can be applied to all kinds of nucleic acid purification equipments for purifying a plurality of biological samples using a magnet rode or a multi-pipette block moving in two or three axial directions, and which can minimize pollution due to the aerosol generated from the biological sample containing high concentration target nucleic acid and also can obtain accurate results.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

The present invention relates to an automatic real-time quantitative amplification system which can perform analysis of various biological samples, and more particularly to an automatic real-time quantitative amplification system in which a plurality of decks for respectively accommodating biological samples are put in a deck storing/transferring device, whereby it is possible to automatically analyze an amount or existence of a target substance containing a target nucleic acid in the biologic sample, such as a particular gene, a particular, a particular pathogenic bacterium and a particular protein, by amplifying the target nucleic acid purified by some processes of purification, purification after culture, or purification after reaction of the target substance contained in the biological sample and then checking an amount of the amplified target nucleic acid.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• B01L 3/02 - Burettes; Pipettes
• C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
• G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
• B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system

Abstract

Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system.

IPC Classes  ?

• A61K 47/00 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
• A61K 9/107 - Emulsions
• A61K 47/48 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives the non-active ingredient being chemically bound to the active ingredient, e.g. polymer drug conjugates
• A61K 47/34 - Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers

Abstract

An automatic biological sample purification apparatus is disclosed. The apparatus is equipped with a magnetic field applying part, in which the magnetic field applying part for purifying biological samples and a heating part are integrally formed with each other so as to be movable up and down. A method of extracting a target substance from the biological sample using the automatic biological sample purification apparatus equipped with the magnetic field applying part is also disclosed.

IPC Classes  ?

• B03C 1/02 - Magnetic separation acting directly on the substance being separated
• C12M 1/32 - Inoculator or sampler multiple field or continuous type
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

There is provided a sample preconcentrator. The sample preconcentrator in which a sample gas injection port is coupled to a dried gas supply source and a gas analysis system to concentrate a sample gas comprises a sample concentrating unit containing an absorbent that is composed of carbon nanotube-metal nanocomplex; a conduit switching valve for selectively coupling the sample gas injection port to the dried gas supply source and the gas analysis system and controlling the absorption and desorption of the sample gas from the sample concentrating unit; and a plurality of conduits for connecting the sample gas injection port, the dried gas supply source, the gas analysis system, the sample concentrating unit and the conduit switching valve.

IPC Classes  ?

• G01N 1/40 - Concentrating samples
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

Provided are an RNase activity inhibitory compound to effectively control the activity of the RNase promoting degradation of extracted RNAs and, in addition, a sample storage container including the same. The RNase activity inhibitory compound and the sample storage container according to the present invention may be effectively used to store RNAs during RNA extraction or the extracted RNAs.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system.

IPC Classes  ?

• A61K 47/00 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
• A61K 9/107 - Emulsions
• A61K 47/48 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives the non-active ingredient being chemically bound to the active ingredient, e.g. polymer drug conjugates
• A61K 47/34 - Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers

Abstract

Provided is a carbon nanostructure-metal composite nanoporous film in which a carbon nanostructure-metal composite is coated on one surface or both surfaces of a membrane support having micro- or nano-sized pores. A method for manufacturing a carbon nanostructure-metal composite nanoporous film, includes: dispersing a carbon nanostructure-metal composite in a solvent at the presence of a surfactant and coating the carbon nanostructure-metal composite on one surface or both surfaces of a membrane support; and fusing the metal on the membrane support by heating the coated membrane support. The metal in carbon nanostructure-metal composite nanoporous film melts at a low temperature since a size of a metal of the carbon nanostructure-metal composite is several nm to several-hundred nm.

IPC Classes  ?

• B01D 71/02 - Inorganic material
• B01D 69/00 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
• B05D 3/02 - Pretreatment of surfaces to which liquids or other fluent materials are to be applied; After-treatment of applied coatings, e.g. intermediate treating of an applied coating preparatory to subsequent applications of liquids or other fluent materials by baking
• B82Y 30/00 - Nanotechnology for materials or surface science, e.g. nanocomposites
• B82Y 40/00 - Manufacture or treatment of nanostructures
• B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
• B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
• B01D 69/10 - Supported membranes; Membrane supports
• B01D 69/12 - Composite membranes; Ultra-thin membranes
• C02F 1/44 - Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
• C02F 1/50 - Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment

Abstract

The present invention relates to a method for preparing a nano-composite comprising carbon nanotube and metal, more precisely a method for preparing a carbon nanotube-metal composite comprising the steps of preparing a dispersion solution by dispersing carbon nanotube in a reductive solvent; preparing a mixed solution by adding a stabilizer and a metal precursor; and reducing the metal precursor by heating the mixed solution, and a carbon nanotube-metal composite prepared by the same. The method of the present invention favors the production of a carbon nanotube-metal composite which is characterized by even metal particles from some nm to hundreds nm in size and even dispersion of those particles to be bound to carbon nanotube.

IPC Classes  ?

• C09K 3/00 - Materials not provided for elsewhere
• B82Y 30/00 - Nanotechnology for materials or surface science, e.g. nanocomposites
• B82Y 40/00 - Manufacture or treatment of nanostructures
• C23C 18/16 - Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by reduction or substitution, i.e. electroless plating
• B22F 1/00 - Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
• B22F 1/02 - Special treatment of metallic powder, e.g. to facilitate working, to improve properties; Metallic powders per se, e.g. mixtures of particles of different composition comprising coating of the powder
• C01B 31/02 - Preparation of carbon; Purification
• C23C 18/08 - Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition characterised by the deposition of metallic material
• C23C 18/10 - Deposition of aluminium only
• C23C 18/31 - Coating with metals

Abstract

The present invention relates to silica magnetic particles having a spherical form and a process for preparing the same. The silica magnetic particles prepared according to the present invention, which are silica particles that includes the magnetic particles and additionally have the functional group on the surfaces, has an advantage that the particle size distribution is uniform. Further, the silica magnetic particles prepared according to the present invention can be used as a reagent for separating biomaterials and a reagent for detecting biomaterials.

IPC Classes  ?

• C01B 33/12 - Silica; Hydrates thereof, e.g. lepidoic silicic acid
• C01B 33/20 - Silicates
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Abstract

The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.

IPC Classes  ?

• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• C07H 19/04 - Heterocyclic radicals containing only nitrogen as ring hetero atom
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

The present invention relates to a micro-chamber plate and a manufacturing method of the same, more precisely a micro-chamber plate facilitating real-time measurement and analysis of fluorescence obtained from the reaction of multiple reaction solutions containing primers or probes selectively binding to each corresponding gene without cross-contamination in order to analyze biological samples containing numbers of genes and a manufacturing method of the same.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• G01N 21/05 - Flow-through cuvettes
• G01N 21/64 - Fluorescence; Phosphorescence
• G01N 21/03 - Cuvette constructions

Abstract

Disclosed is a cyclic reverse transcription method, which comprises performing reverse transcription reaction in one or more cycles, each cycle comprising the steps of: (i) performing reaction with a reverse transcription reaction solution comprising template RNA, RNA-dependent DNA polymerase, a reaction buffer, primers for reverse transcription and dNTPs, and optionally, a stabilizer, at 10° C. to 40° C. and, (ii) performing reaction with the resultant reaction solution at 42° to 55° C.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long-term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

Lactobacillus gasseri BNR17 of the present invention has excellent acid resistance, bile acid resistance, enteric absorption activity and antimicrobial activity against pathogenic microorganisms, in addition to the weight gaining inhibitory effect by synthesizing indigestible polysaccharides from monosaccharides included in food taken and releasing the synthesized polysaccharides out of the body. Therefore, the strain of the invention, owing to such beneficiary effects, can be effectively used not only for the production of fermented milk, other fermented food products and animal feeds but also for the production of live cell products and food additives for preventing weight gaining.

IPC Classes  ?

• A61K 35/74 - Bacteria
• C12N 1/20 - Bacteria; Culture media therefor

Abstract

A method for isolating a nucleic acid from a biological sample includes applying particulate matter to promote co-aggregation and co-precipitation of insoluble aggregate by directly adding to the biological sample, adding to the biological sample in admixture with a cell lysis buffer, adding to the biological sample treated with a cell lysis buffer, adding to cell lysates in admixture with a buffer for forming denatured protein aggregate; or adding to cell lysates comprising the formed denatured protein aggregate. The particulate matter is selected from the group consisting of a material formed from an element of Ag, Fe, Ti, Al, Sn, Si, Cu, Mo, Ni, W or Zn, an oxide, a carbide, a nitride, a boride and a silicide thereof, and a mixture thereof, a polymer selected from PMMA (Poly Methyl MethAcrylate), polyethylene or polyurethane; and a mixture thereof. The insoluble aggregate comprises denatured protein aggregate and cell debris.

IPC Classes  ?

• C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

The present invention relates to a real-time PCR monitoring apparatus for real-time monitoring production of reaction product produced during the reaction while performing nucleic acid amplification such as PCR for various kinds of trace samples. Specifically, the present invention relates to an apparatus for real-time monitoring biochemical reaction for efficiently dividing interference between an excitation light and a fluorescence, which includes a polarizer, a polarizing beam splitter, a polarization converter and so on.

IPC Classes  ?

• G01J 4/00 - Measuring polarisation of light

Abstract

The present invention relates to a dried oligonucleotide composition and a method for producing the same. More specifically, it relates to a dried oligonucleotide composition produced by the steps comprising adding a substance for preventing the oligonucleotide from being separated and lost, which is adhesive to a storage container containing the oligonucleotide composition, in order to prevent the oligonucleotide from being separated and lost during manufacturing and distributing the dried oligonucleotide composition, optionally adding a non-reactive dye substance, and drying the resulting solution. The dried oligonucleotide composition of the present invention can be prevented from being separated and lost during manufacturing step, or transporting step after packaging, and the presence or absence of the oligonucleotide in the storage container can be easily confirmed with naked eyes. Accordingly, unnecessary labor waste and time waste caused by the separation of the oligonucleotide upon experiment can be overcome.

IPC Classes  ?

• C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

The present invention is related to hybrid conjugates formed by covalently bonding siRNA (small interfering RNA) molecules to hydrophilic polymers for improving stability of the siRNA molecules effective for delivering the siRNA in vivo, and polyelectrolyte complex micelles formed by ionic interactions between the conjugates and multifunctional cationic compounds. The siRNA-hydrophilic polymer conjugates and polyelectrolyte complex micelles derived therefrom can be used for improving stability of the siRNA molecules in vivo. Consequently, the delivery of siRNA molecules for therapeutic applications into cells can be facilitated, and the siRNA is still active even though a small dose of the siRNA is used.

IPC Classes  ?

• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Abstract

Disclosed is a conjugate for gene transfer, which is capable of being used for treatment of incurable diseases, comprising an oligonucleotide intended to be transferred into target cells and a hydrophilic polymer, wherein an end of the oligonucleotide is covalently conjugated to the hydrophilic polymer. Also, the present invention discloses polyelectrolyte complex micelles formed from such a conjugate and a cationic polymer or cationic peptide. Such polyelectrolyte complex micelles can effectively transfer oligonucleotides as therapeutic agents into target cells, making it possible to obtain desired activities of the delivered oligonucleotides in target cells even when the micelles are clinically applied at a relatively low concentration. Therefore, the conjugate and the polyelectrolyte complex micelle are very useful in basic life science research and the medical field.

IPC Classes  ?

• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Abstract

The present invention relates to an apparatus for real-time monitoring chemical reaction between various biomaterials. More particularly, the present invention directed to a real-time monitoring apparatus for biochemical reaction, which comprises parabolic mirror and/or an optical waveguide tube for effective irradiation of light over the whole plate with uniform intensity.

IPC Classes  ?

• G01J 3/00 - Spectrometry; Spectrophotometry; Monochromators; Measuring colours