Methods and compositions comprising primers and probes to detect nucleic acids are provided. The probes comprise a ribonucleotide that can be cleaved by an RNase H2 enzyme when the probe is annealed to a reverse complement of a universal sequence that is introduced to a target nucleic acid, for example via amplification.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12Q 1/6816 - Hybridisation assays characterised by the detection means
2.
UNIVERSAL FLUORESCENT PROBES ACTIVATED WITH RNaseH2
Methods and compositions comprising primers and probes to detect nucleic acids are provided. The probes comprise a ribonucleotide that can be cleaved by an RNase H2 enzyme when the probe is annealed to a reverse complement of a universal sequence that is introduced to a target nucleic acid, for example via amplification.
The invention provides a method of diagnosing Non-Alcoholic Steatohepatitis (NASH) and/or the hepatic fibrosis status of a subject, especially a subject afflicted with Non-alcoholic fatty liver disease (NAFLD) or NASH, based on the level of only three or more particular biomarkers. The invention further provides a kit suitable for performing said method and the use of said method and methods of treating patients diagnosed in accordance with the disclosed methods.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
G16B 20/00 - ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
C12Q 1/6816 - Hybridisation assays characterised by the detection means
Methods and systems for thermally controlling a chemical reaction in droplets. In an exemplary method, a first thermal zone and a second thermal zone having different temperatures from one another may be created in a reaction chamber. An emulsion including droplets encapsulated by a carrier fluid may be held in the reaction chamber. The droplets may have a density mismatch with the carrier fluid, and each droplet may include one or more reactants for the chemical reaction. An orientation of the reaction chamber may be changed to move the droplets from the first thermal zone to the second thermal zone, such that a rate of the chemical reaction changes in at least a subset of the droplets.
The digital amplification methods and kits provide the ability to estimate the fetal fraction of cell-free DNA (cfDNA) in a maternal sample, e.g., plasma or serum, by analysis of target sites that are differentially methylated in fetal and maternal cfDNA.
Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
A system and method for SARS-CoV-2 variant classification through mutation detection from qPCR fluorescence signals. The system receives fluorescence signals, wherein a first fluorescence signal indicates quantitative presence of a first genomic region as a control for SARS-CoV-2, and a second fluorescence signal indicates quantitative presence of a second genomic region of a first mutation present in a subset of variants of SARS-CoV- 2. The system measures a first Cq for the first fluorescence signal and a second Cq for the second fluorescence signal as the signals cross a threshold RFU. The system calculates a delta. Cq as a difference between the two. Further, the system identifies a first peak RFU for the first fluorescence signal and a second peak RFU for the second fluorescence signal, and calculates a RFU ratio of the two. The system detects presence of the first mutation based on the delta Cq and/or the RFU ratio.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism
A system and method for SARS-CoV-2 variant classification through mutation detection from qPCR fluorescence signals. The system receives fluorescence signals, wherein a first fluorescence signal indicates quantitative presence of a first genomic region as a control for SARS-CoV-2, and a second fluorescence signal indicates quantitative presence of a second genomic region of a first mutation present in a subset of variants of SARS-CoV-2. The system measures a first Cq for the first fluorescence signal and a second Cq for the second fluorescence signal as the signals cross a threshold RFU. The system calculates a delta Cq as a difference between the two. Further, the system identifies a first peak RFU for the first fluorescence signal and a second peak RFU for the second fluorescence signal, and calculates a RFU ratio of the two. The system detects presence of the first mutation based on the delta Cq and/or the RFU ratio.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
14.
SYSTEM AND METHOD FOR TARGET MATERIAL RETRIEVAL FROM MICROWELLS
A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
A clinical diagnostic analyzer for performing an automated crossover study on quality control (QC) material includes a processor, memory, measurement hardware, and an input panel/display. The analyzer prompts a user to load a QC specimen, and to instigate testing and analysis to determine a mean and a standard deviation for the new material. Associated methods for using one or more clinical diagnostic analyzers to calculate a new mean and standard deviation for a new QC material, reduce error in the calculated mean value, and to reduce the total number of days to complete a crossover study are also disclosed.
Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.
C09D 183/00 - Coating compositions based on macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon, with or without sulfur, nitrogen, oxygen, or carbon only; Coating compositions based on derivatives of such polymers
B81C 1/00 - Manufacture or treatment of devices or systems in or on a substrate
18.
INSTRUMENT WITH INTEGRATED CLAMP AND LATCH WITH CAM OPTIMIZED FOR MULTIPLE CONSUMABLE HEIGHTS
An instrument (e.g., a thermal cycler) includes a consumable compartment configured to receive multiple height consumables, and a lid for opening or closing the consumable compartment. The lid includes a motor, a pressure plate, a compression bar, and a cam and a follower. The compression bar has a first side (e.g., bottom) coupled to the pressure plate and a second side (e.g., top) at which the cam and the follower is disposed. The cam is drivably coupled to the motor and the follower is coupled to the compression bar to induce translation of the compression bar when engaged with the cam. The cam includes a cam profile configured to adjust a height of the pressure plate between different height consumables to apply a pre-determined pressure. The motor can also operate a latch assembly to latch the lid before activating the cam and follower assembly.
Systems and methods for performing testing of a single analyte on a group of multiple clinical diagnostic analyzers, or a single clinical diagnostic analyzer having multiple analytic units, or combinations thereof, are disclosed. A mean and SD for each individual instrument are input to at least one of the instruments along with a QC rule to be used, the probability of false rejection function for the QC rule, and a desired false rejection rate. A group mean and a group SD are calculated to satisfy the desired false rejection rate and QC rule and loaded into each individual instrument for use in testing the single analyte at each individual instrument.
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G16H 40/40 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the management of medical equipment or devices, e.g. scheduling maintenance or upgrades
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
20.
METHODS FOR PRODUCING MODIFIED REVERSE TRANSCRIPTASES
The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.
Systems and methods for conducting customized automated crossover studies for clinical diagnostic analyzers are disclosed. In disclosed embodiments, an optimal number of samples to run on a new QC material is determined based on historical information of the analyzer instrument and a peer group of similar instruments. Using the determined optimal number of samples, the crossover study is conducted, and the results are reported.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Systems, including methods and apparatus, for analyzing partitioned samples, such as droplets, using recirculated fluid. The systems may be used to analyze a plurality of partitioned samples, with fluid used with initial samples reused with later samples. This reuse, or recirculation, may reduce the amount of fluid required for performing multiple analyses, with concomitant reductions in costs. Moreover, in some embodiments, it may simplify operation by increasing the number of analyses that may be performed before fluid must be replenished or replaced.
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
Systems, including methods and apparatus, for analyzing partitioned samples, such as droplets, using recirculated fluid. The systems may be used to analyze a plurality of partitioned samples, with fluid used with initial samples reused with later samples. This reuse, or recirculation, may reduce the amount of fluid required for performing multiple analyses, with concomitant reductions in costs. Moreover, in some embodiments, it may simplify operation by increasing the number of analyses that may be performed before fluid must be replenished or replaced.
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
The subject invention pertains to proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic 1,3-dioxoisoindolin-2-yl group functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of one, two, three, four, or five atoms between the hydrophobic group and the support matrix.
B01J 20/289 - Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
B01J 20/30 - Processes for preparing, regenerating or reactivating
C07K 1/16 - Extraction; Separation; Purification by chromatography
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
B01J 20/28 - Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
27.
USE OF HOMOLOGOUS RECOMBINASE TO IMPROVE EFFICIENCY AND SENSITIVITY OF SINGLE CELL ASSAYS
Methods of decreasing bead aggregation and improving specificity of nucleic acid hybridization using non-specific nucleic acid binding proteins is described.
The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.
G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
B01F 33/3011 - Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
B01F 33/302 - Micromixers the materials to be mixed flowing in the form of droplets
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
C40B 40/00 - Libraries per se, e.g. arrays, mixtures
C40B 40/04 - Libraries containing only organic compounds
C40B 70/00 - Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or barcodes
G01N 33/532 - Production of labelled immunochemicals
30.
USE OF HOMOLOGOUS RECOMBINASE TO IMPROVE EFFICIENCY AND SENSITIVITY OF SINGLE CELL ASSAYS
Methods of decreasing bead aggregation and improving specificity of nucleic acid hybridization using non-specific nucleic acid binding proteins is described.
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Computer software for use in collection, statistical
analysis and management of external quality assessment data
from clinical laboratories. Application service provider featuring software for use in
the collection, statistical analysis and management of
clinical laboratory data.
32.
SYSTEM AND METHOD FOR LEAKAGE CONTROL IN A PARTICLE CAPTURE SYSTEM
A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.
One or more instruments generate test result data. The test result data include patient data and QC data. The test result data is provided to a QC data flow system via a local network, which filters the test result data (e.g., using a set of rules) to extract the QC data. The QC data is provided to a cloud-based QC data management platform via an external network. The cloud-based QC data management platform analyzes the QC data and provides a result back to the QC data flow system. The QC data flow system forwards the result to middleware or the instrument, which triggers a corrective action based on the result as appropriate.
G16H 40/40 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the management of medical equipment or devices, e.g. scheduling maintenance or upgrades
G06Q 10/06 - Resources, workflows, human or project management; Enterprise or organisation planning; Enterprise or organisation modelling
G06F 15/16 - Combinations of two or more digital computers each having at least an arithmetic unit, a program unit and a register, e.g. for a simultaneous processing of several programs
Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.
Method and system for detecting reverse transcriptase activity of a sample by digital assay. In an exemplary method, a reaction mixture including the sample, an RNA polymer, and reagents for reverse transcription may be prepared. Complementary DNA (cDNA) may be synthesized in the reaction mixture using the RNA polymer as a template. An amount of the cDNA synthesized may be proportional to the reverse transcriptase activity of the sample. Partitions may be formed after synthesizing the cDNA. The partitions may contain copies of the cDNA at partial occupancy. Each partition may include a portion of the reaction mixture. A target representing the cDNA may be amplified in the partitions. Amplification data for the target may be collected from the partitions.
Method and system for detecting reverse transcriptase activity of a sample by digital assay. In an exemplary method, a reaction mixture including the sample, an RNA polymer, and reagents for reverse transcription may be prepared. Complementary DNA (cDNA) may be synthesized in the reaction mixture using the RNA polymer as a template. An amount of the cDNA synthesized may be proportional to the reverse transcriptase activity of the sample. Partitions may be formed after synthesizing the cDNA. The partitions may contain copies of the cDNA at partial occupancy. Each partition may include a portion of the reaction mixture. A target representing the cDNA may be amplified in the partitions. Amplification data for the target may be collected from the partitions.
The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
B01J 19/00 - Chemical, physical or physico-chemical processes in general; Their relevant apparatus
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
C40B 40/04 - Libraries containing only organic compounds
C40B 50/08 - Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
C40B 60/10 - Apparatus specially adapted for use in combinatorial chemistry or with libraries for identifying library members
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
B01F 25/433 - Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
B01F 33/3011 - Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
C12Q 1/25 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups
C12Q 1/6804 - Nucleic acid analysis using immunogens
C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism
G01N 33/542 - Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/573 - Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
41.
METHODS AND HYDROGEL COMPOSITIONS FOR PARTITIONING BIOLOGICAL SAMPLES
Methods of making and forming partitions in nanovials are provided. Wherein linking oligonucleotides to cell nucleic acids in hollow nanovials, the method comprising, loading cells into openings in hollow nanovials; inserting one or more hydrogel bead into the openings, thereby occluding the openings and preventing diffusion the cells from the openings is disclosed.
Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
B01F 33/81 - Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles
B01F 33/3011 - Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
Systems and methods for automated digital polymerase chain reaction are described herein. A method of performing automated digital Polymerase Chain Reaction (PCR) can include receiving a sample in a sample tube within a sample tube holder of an automated digital PCR system. The automated digital PCR system can include a multichannel pipettor, a heater that can thermocycle samples in a PCR cartridge, and an imager. The method can include performing pipetting operations with the multi-channel pipettor to transfer a portion of the sample to a PCR cartridge, thermocycling the sample in the PCR cartridge with the heater, and imaging the sample in the PCR cartridge with the imager.
A method comprises providing a microfluidic device including a reservoir; an injection nozzle at the bottom of the reservoir, the injection nozzle being wider at its top than at its bottom; an injection channel below the injection nozzle; and a microfluidic channel below the injection channel. The method further comprises placing fluid in the reservoir, and allowing the fluid to passively flow through the injection nozzle and the injection channel.
Methods, systems, and devices for sampling/isolating material from cells. An exemplary system may comprise a chip including an electrode array of sampling electrodes arranged along a surface of the chip. A cell-receiving area may be located adjacent the surface of the chip. The system also may comprise a tag array of tags supported by the chip and aligned with the electrode array. Each tag of the tag array may include an identifier that is unique to the tag within the tag array. Each tag may be configured to bind nucleic acids, or a capturing agent distinct from the tag may be aligned with each sampling electrode of the electrode array to capture a protein or other analyte of interest. The system further may comprise a control circuit configured to apply an individually controllable voltage to each sampling electrode of the electrode array and measure an electrical property of the sampling electrode.
In one implementation, a microfluidic probe has a non-planar processing surface and an inlet aperture. The shape of the surface may be selected to produce a specific velocity gradient profile across a surface onto which fluid is deposited using the microfluidic probe, for example a constant velocity gradient or a velocity gradient that decreases linearly with distance from the inlet aperture. The microfluidic probe may define and overflow notch in a perimeter edge of the processing surface.
Methods and systems for detecting particles. In an exemplary method, a sample fluid including the particles may be driven from a sample inlet channel, through a confluence region, and into a sample outlet channel defining a longitudinal axis. Focusing fluid may be introduced into the confluence region from at least two focusing channels along respective introduction axes. Introducing may be rotationally asymmetrical about the longitudinal axis. The introduction axes and the longitudinal axis may collectively extend in three dimensions. The particles may be passed through an interrogation zone of the sample outlet channel. The interrogation zone may be irradiated with light. Optical radiation may be detected from the interrogation zone.
Methods and systems for detecting particles. In an exemplary method, a sample fluid including the particles may be driven from a sample inlet channel, through a confluence region, and into a sample outlet channel defining a longitudinal axis. Focusing fluid may be introduced into the confluence region from at least two focusing channels along respective introduction axes. Introducing may be rotationally asymmetrical about the longitudinal axis. The introduction axes and the longitudinal axis may collectively extend in three dimensions. The particles may be passed through an interrogation zone of the sample outlet channel. The interrogation zone may be irradiated with light. Optical radiation may be detected from the interrogation zone.
G01N 33/557 - Immunoassay; Biospecific binding assay; Materials therefor using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
G01N 33/536 - Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
G01N 33/544 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
C12M 1/00 - Apparatus for enzymology or microbiology
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Methods, compositions, and kits for analyzing viral particles. In an exemplary method of analyzing viral particles, capsids of the viral particles may be tagged with a tag. Subsamples of a sample containing the viral particles may be formed. Each subsample of only a subset of the subsamples may include at least one of the viral particles. One or more targets may be amplified from a genome of the viral particles. Tag-related data, and amplification data for the one or more targets, may be collected from the subsamples. In some examples, a capsid occupancy of the viral particles may be determined in a calibration-free approach using the collected data without quantification of the viral particles or capsids thereof.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
G01N 33/569 - Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
Methods, systems, and computer-readable media for sizing a population of nucleic acid fragments. In an exemplary method of sizing a population of nucleic acid fragments provided by a sample, partitions may be formed, each containing a portion of the sample. Each target of at least two targets may be present in only a fraction of the nucleic acid fragments. Amplification reactions may be performed for each of the at least two targets in the partitions. Amplification data may be collected for the at least two targets from the partitions. Target-defined subsets of the partitions may be enumerated using the amplification data. At least one length characteristic of the population of nucleic acid fragments may be predicted using results of enumerating.
The methods allow for provision of a mixture of a plurality of beads, each bead linked to oligonucleotides, wherein the mixture can be treated as a bulk solution (prior to partitioning) to cleave a covalent bond linking the oligonucleotides to the beads while retaining a non-covalent linkage (via hybridization) between the beads and the oligonucleotides, allowing for distribution of the oligonucleotides and beads to partitions or 2D arrays prior to separation of the oligonucleotides from the beads, which occurs for example in the partitions.
Methods, compositions, and kits for analyzing viral particles. In an exemplary method of analyzing viral particles, capsids of the viral particles may be tagged with a tag. Subsamples of a sample containing the viral particles may be formed. Each subsample of only a subset of the subsamples may include at least one of the viral particles. One or more targets may be amplified from a genome of the viral particles. Tag-related data, and amplification data for the one or more targets, may be collected from the subsamples. In some examples, a capsid occupancy of the viral particles may be determined in a calibration-free approach using the collected data without quantification of the viral particles or capsids thereof.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
Methods, systems, and computer-readable media for sizing a population of nucleic acid fragments. In an exemplary method of sizing a population of nucleic acid fragments provided by a sample, partitions may be formed, each containing a portion of the sample. Each target of at least two targets may be present in only a fraction of the nucleic acid fragments. Amplification reactions may be performed for each of the at least two targets in the partitions. Amplification data may be collected for the at least two targets from the partitions. Target-defined subsets of the partitions may be enumerated using the amplification data. At least one length characteristic of the population of nucleic acid fragments may be predicted using results of enumerating.
The methods allow for provision of a mixture of a plurality of beads, each bead linked to oligonucleotides, wherein the mixture can be treated as a bulk solution (prior to partitioning) to cleave a covalent bond linking the oligonucleotides to the beads while retaining a non-covalent linkage (via hybridization) between the beads and the oligonucleotides, allowing for distribution of the oligonucleotides and beads to partitions or 2D arrays prior to separation of the oligonucleotides from the beads, which occurs for example in the partitions.
Disclosed is a system and method for dynamically adjusting analytical precision of a clinical diagnostic process. The system and method employ a clinical diagnostic analyzer that evaluates a sample and determines whether the resultant value, or mean value, is within a predetermined window or within a threshold of a predetermined value of a precision profile. If so, the analyzer automatically and dynamically determines a desired analytical precision and conducts additional testing of replicate samples to achieve a desired precision and reports the results to a user.
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
G01N 33/48 - Biological material, e.g. blood, urine; Haemocytometers
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups
G16H 15/00 - ICT specially adapted for medical reports, e.g. generation or transmission thereof
G16H 40/40 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the management of medical equipment or devices, e.g. scheduling maintenance or upgrades
G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
C12Q 1/683 - Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism
The present invention relates in general to microscopy systems. In particular, the present invention relates to microscopes rendering digital images of samples, with the capability to digitally control the focus of the microscope system, and the software used to control the operation of the digital microscope system. Further, the present invention relates to a microscope structure that allows for compact and multi-functional use of a microscope, providing for light shielding and control with samples that require specific light wavelength characteristics, such as fluorescence, for detection and imaging. The microscope is adjustable, with a structure that can move along range(s) of motion and degree(s) of freedom to allow for ease of access to samples, shielding of samples, and manipulation of a display apparatus.
The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.
A wide-spectrum analysis system. The system may comprise various components, including a stage, a detection module, and an optical relay structure. The stage may be configured to support a sample holder—a gel or blot, a PCR plate or microplate, sample chips, or a microfluidic device, among others—at an examination region. The detection module may be configured to detect light originating from one or more samples positioned in the sample holder. The detection module may be configured to detect light having wavelengths between about 200 nm and about 2000 nm, or subsets thereof. The optical relay structure may be configured to direct the output light from the examination region to the detection module. The system may further comprise an illumination module. Embodiments of the analyzer may be suitable for use with one or more of the following interrogation formats, among others: chemiluminescence, fluorescence, colorimetry, and spectrometry.
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
The subject application pertains to compositions and methods for enhancing reverse transcriptase (RT) activity and/or reducing the inhibition of RT by inhibitors, such as formalin, tannic acid and/or heparin. In some embodiments, RT inhibition is reduced by the addition of potassium glutamate, histidine hydrochloride monohydrate, poloxamer 188, or any combination thereof to a reaction mixture comprising a polymerase. In other embodiments, RT is enhanced through the addition of a polyvinyl sulfonic acid sodium salt (PVSA) to a reaction mixture. The subject application also provides oligonucleotide primers for use in the reverse transcription of target sequences and its enhancement. These primers have high GC content or low GC content. Methods of using a RT inhibition reducer or a RT enhancer in a composition with an RNA template and RT improves RT yield, RT sensitivity, or RT tolerance to various chemicals are also provided.
A segmentation system processes images of a sample that includes fluorescent entities. The segmentation system applies a threshold image that represents background illumination distinct from fluorescence of target entities to pre-process the images. The segmentation system determines numbers of target entities in pre-processed images and determines whether an estimated number of target entities in the sample meets a threshold certainty. The segmentation system continues to analyze one or more images until the threshold certainty is determined. When the threshold certainty is met, the estimated number of target entities may be used to generate a user interface output (e.g., displaying the pre-processed images and visual indicators of the locations of target entities.
A segmentation system processes images of a sample that includes fluorescent entities. The segmentation system applies a threshold image that represents background illumination distinct from fluorescence of target entities to pre-process the images. The segmentation system determines numbers of target entities in pre-processed images and determines whether an estimated number of target entities in the sample meets a threshold certainty. The segmentation system continues to analyze one or more images until the threshold certainty is determined. When the threshold certainty is met, the estimated number of target entities may be used to generate a user interface output (e.g., displaying the pre-processed images and visual indicators of the locations of target entities).
Methods and compositions for determining the proximity of two barcoding oligonucleotides (e.g., in a single partition or adjacent on a tissue section) using a determination of the presence of a 9 bp sequence resulting from tagmentation in different nucleic acid fragments linked to different barcoding oligonucleotides is provided.
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6804 - Nucleic acid analysis using immunogens
G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
C40B 50/08 - Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.
C40B 50/08 - Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
C40B 40/04 - Libraries containing only organic compounds
B01J 19/00 - Chemical, physical or physico-chemical processes in general; Their relevant apparatus
B01F 25/433 - Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01F 33/3011 - Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
B01F 101/23 - Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
Systems and methods for contact imaging of stands with illumination are disclosed herein. A contact imaging system can include a contact imager. The contact imager can include a housing having a base and a lid. The lid can have a closed position against the base and an open position. The contact imager can include a contact area image sensor. The lid can shield the contact area image sensor from ambient light when the lid is in the closed position. The contact imager can include an illuminator that can illuminate at least a portion of a blot on the contact area image sensor when the lid is in the closed position.
Systems and methods for multiplex detection through measurement of localized fluorescence ratios are disclosed herein. This can include creating a plurality of capture structures that each include a detection portion that can couple with a target analyte and a stem that can include a capture structure code uniquely identifying a type of the capture structure. The capture structures can be attached to a sample surface and mixed with a sample containing a plurality of target analytes. A location and the capture structure code of each of the capture structures can be determined. A location at which a target analyte is bound to one of the capture structures can be identified, and the target analyte can be determined based on the capture structure code of the capture structure at the location at which the target analyte is bound to one of the capture structures.
G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6816 - Hybridisation assays characterised by the detection means
C12Q 1/6832 - Enhancement of hybridisation reaction
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
C12Q 1/6804 - Nucleic acid analysis using immunogens
C12Q 1/6811 - Selection methods for production or design of target specific oligonucleotides or binding molecules
C12Q 1/683 - Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
Reagents, and kits consisting primarily of reagents or of
reagents and sample preparation containers, for use in
scientific and medical research; reagents for scientific and
research use for preparation, handling, amplification, and
analysis of samples containing nucleic acids for use in the
industries of agriculture, biodefense, food science,
forensics, horticulture, medicine, pharmaceuticals, and
toxicology, all in connection with the preparation, handling
amplification, and analysis of samples containing nucleic
acids. Apparatus, namely, medical laboratory research instruments
and scientific laboratory research instruments for
preparation, handling, amplification, and analysis of
samples containing nucleic acids and used in the industries
of agriculture, biodefense, food science, forensics,
horticulture, medicine, pharmaceuticals, and toxicology;
software for preparing, handling, amplification and analysis
of samples containing nucleic acids, for use in scientific
and medical research and used in the industries of
agriculture, biodefense, food science, forensics,
horticulture, medicine, pharmaceuticals, and toxicology;
accessories in the nature of laboratory apparatus, namely,
sample preparation containers and kits consisting primarily
of sample preparation containers and reagents, and
instruction manuals sold as a unit therewith, used to
prepare laboratory samples for use in scientific and medical
research, and in the industries of agriculture, biodefense,
food science, forensics, horticulture, medicine,
pharmaceuticals, and toxicology, all in connection with the
preparation, handling, amplification, and analysis of
samples containing nucleic acids.
Systems and methods for contact imaging of stands with illumination are disclosed herein. A contact imaging system can include a contact imager. The contact imager can include a housing having a base and a lid. The lid can have a closed position against the base and an open position. The contact imager can include a contact area image sensor. The lid can shield the contact area image sensor from ambient light when the lid is in the closed position. The contact imager can include an illuminator that can illuminate at least a portion of a blot on the contact area image sensor when the lid is in the closed position.
Systems and methods for multiplex detection through measurement of localized fluorescence ratios are disclosed herein. This can include creating a plurality of capture structures that each include a detection portion that can couple with a target analyte and a stem that can include a capture structure code uniquely identifying a type of the capture structure. The capture structures can be attached to a sample surface and mixed with a sample containing a plurality of target analytes. A location and the capture structure code of each of the capture structures can be determined. A location at which a target analyte is bound to one of the capture structures can be identified, and the target analyte can be determined based on the capture structure code of the capture structure at the location at which the target analyte is bound to one of the capture structures.
Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.
Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.
B01J 8/00 - Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes
B01J 8/02 - Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes with stationary particles, e.g. in fixed beds
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
(1) Reagents, and kits consisting primarily of reagents or of reagents and sample preparation containers, for use in scientific and medical research; reagents for scientific and research use for preparation, handling, amplification, and analysis of samples containing nucleic acids for use in the industries of agriculture, biodefense, food science, forensics, horticulture, medicine, pharmaceuticals, and toxicology, all in connection with the preparation, handling amplification, and analysis of samples containing nucleic acids.
(2) Apparatus, namely, medical laboratory research instruments and scientific laboratory research instruments for preparation, handling, amplification, and analysis of samples containing nucleic acids and used in the industries of agriculture, biodefense, food science, forensics, horticulture, medicine, pharmaceuticals, and toxicology; software for preparing, handling, amplification and analysis of samples containing nucleic acids, for use in scientific and medical research and used in the industries of agriculture, biodefense, food science, forensics, horticulture, medicine, pharmaceuticals, and toxicology; accessories in the nature of laboratory apparatus, namely, sample preparation containers and kits consisting primarily of sample preparation containers and reagents, and instruction manuals sold as a unit therewith, used to prepare laboratory samples for use in scientific and medical research, and in the industries of agriculture, biodefense, food science, forensics, horticulture, medicine, pharmaceuticals, and toxicology, all in connection with the preparation, handling, amplification, and analysis of samples containing nucleic acids.
88.
Circuit for sharing current between parallel LEDs or parallel strings of LEDs
A circuit for sharing current between parallel LEDs or parallel strings of LEDs, and a method of use of the same, are disclosed herein. The circuit for sharing current between parallel LED pathways can include a first LED pathway, a first transistor coupled to the first set of LEDs and that can control a first current through the first set of LEDs, and a first measurement node having a first sensed voltage. The circuit can include a second LED pathway, a second transistor coupled to the second set of LEDs and that can control a second current through the second set of LEDs, and a second measurement node having a second sensed voltage. The circuit includes a first differential amplifier and a second differential amplifier, each of which can compare sensed voltage and can apply a voltage to a gate of one of the first and second transistors.
H05B 45/46 - Circuit arrangements for operating light-emitting diodes [LED] - Details of LED load circuits with an active control inside an LED matrix having LEDs disposed in parallel lines
89.
METHODS AND COMPOSITIONS FOR GENOTYPING AND PHENOTYPING CELLS
A heat pump that includes a thermoelectric device(s) and a heat sink having a raised portion with a top surface for thermally coupling with a planar face of the thermoelectric device(s). The raised portion of the heat sink includes an outer periphery and a raised central region surrounded by a void region to provide more uniform thermal conductivity when clamped within an assembly. The raised central region is shaped in an any shape corresponding to a shape of uneven thermal conductivity due to clamping pressure applied to the heat sink. The void region can be substantially contiguous and entirely circumscribe the central raised region. The device can optionally include discrete supports formed of a less thermally-conductive material within the void region. The supports can be elastomeric, such as O-rings, and disposed within pockets defined within the void region.
F25B 21/04 - Machines, plants or systems, using electric or magnetic effects using Nernst-Ettinghausen effect reversible
B01L 7/00 - Heating or cooling apparatus; Heat insulating devices
H10N 10/10 - Thermoelectric devices comprising a junction of dissimilar materials, i.e. devices exhibiting Seebeck or Peltier effects operating with only the Peltier or Seebeck effects
09 - Scientific and electric apparatus and instruments
Goods & Services
Apparatus, namely, medical laboratory research instruments and scientific laboratory research instruments for preparation, handling, amplification, and analysis of samples containing nucleic acids and used in the industries of agriculture, biodefense, food science, forensics, horticulture, medicine, pharmaceuticals, and toxicology; downloadable software for preparing, handling, amplification and analysis of samples containing nucleic acids, for use in scientific and medical research and used in the industries of agriculture, biodefense, food science, forensics, horticulture, medicine, pharmaceuticals, and toxicology
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Reagents, and kits consisting primarily of reagents or of reagents and sample preparation containers, for use in scientific and medical research; and reagents for scientific and research use for preparation, handling, amplification, and analysis of samples containing nucleic acids for use in the industries of agriculture, biodefense, food science, forensics, horticulture, medicine, pharmaceuticals, and toxicology, all in connection with the preparation, handling amplification, and analysis of samples containing nucleic acids
94.
MIXED MODE CATIONIC EXCHANGE CHROMATOGRAPHY LIGANDS BASED ON SUBSTITUTED 2-BENZAMIDO ACETIC ACID STRUCTURES
The subject invention pertains to mixed mode chromatography ligands and chromatography matrices suitable for the purification of proteins from biological sources or biological samples. Methods of making chromatography matrices comprising the disclosed ligands and using the disclosed chromatography matrices are also provided.
A fluorescence detection system, including apparatus and methods, suitable for qPCR and other fluorescence-based analyses. The system may comprise various components, including a stage, an illumination module, a detection module, and an optical relay structure. The stage may be configured to support a sample holder. The illumination module may include one or more discrete light sources configured to produce excitation light. The detection module may be configured to detect fluorescence emission light produced, in response to the excitation light, by a fluorescent sample positioned in the sample holder. The optical relay structure may include a beamsplitter assembly configured to direct the excitation light from the illumination module along an illumination path to the sample holder and to direct the fluorescence emission light from the sample holder along a response path to the imaging module. The system may enhance the quality of excitation light hitting samples in the sample holder, for example, by collimating and/or homogenizing the light.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
(1) Fluorescent dyes for scientific or research use; fluorescent dyes for scientific use in life science applications; dyes, stains, namely, fluorescent dyes and chemicals for labeling or staining biological cells for scientific, laboratory, or research purposes; fluorescent dyes for research use in fluorescent immunoassays (FLISA), cell-based assays, flow cytometry, and fluorescent immunohistochemical staining, and in vivo imaging; proteins for scientific and research use; cell culture proteins for scientific and research use; biochemical, namely, monoclonal antibodies for in vitro scientific and research use; biochemically labelled and enzyme labelled antibodies for use in science; microparticles containing fluorescent dyes for flow cytometry and scientific use; enzyme and chemical labelling molecules for use in science; chemicals, reagents, chemical preparations, and chemical substances all for use in analysis and testing in laboratories other than for medical or veterinary purposes; assays and reagents for use in scientific, laboratory and research purposes; chemicals, reagents, chemical preparations, and chemical substances all for use in analysis and testing in laboratories other than for medical or veterinary purposes; immunoassays in the nature of chemical tests used to detect or quantify analytes using an immunological reaction; chemical agents for use in separation for scientific and research use; cell culture media for use in separation techniques for scientific and research use; chemical reagent for research purposes, namely, a bioconjugation system for conjugation and immobilization of peptides, fluorophores, biomolecules, proteins, carbohydrates, DNA/RNA or other molecules; biochemicals for scientific, laboratory, and research use, namely, proteolytically-activatable, protease-activatable, or proteolytically activated or protease-activated immunoglobulin and antigen-binding fragments for binding to tissue, cells and antigens; biochemical test kits comprising assays and reagents for scientific, laboratory and research purposes; assay kits comprising assays and reagents for measuring, monitoring, testing and analyzing proteolytic activation and antibody binding for scientific, laboratory and research use; conjugation and labelling kits comprised primarily of reagents, antibodies, labelling enzymes and chemicals for performing conjugation and labelling reactions for use in science; reagents in kit form for performing assays for scientific and research use; flow cytometry kits comprised of research reagents, buffers and controls for research laboratory use.
(2) Fluorescent dyes and chemicals for medical use; dyes, stains, namely, fluorescent dyes and chemicals for labeling or staining biological cells for medical purposes; proteins for medical use; cell culture proteins for medical use; monoclonal antibodies for medical use; monoclonal antibodies for in vitro medical use; fluorescent dyes for medical use in fluorescent immunoassays (FLISA), cell-based assays, flow cytometry, and fluorescent immunohistochemical staining, and in vivo imaging; biochemically labelled and enzyme labelled antibodies for medical use; microparticles containing fluorescent dyes for flow cytometry and medical use; chemical and biochemical preparations, assays, dyes, and reagents for clinical and medical laboratory use; chemical reagents for medical purposes for flow cytometry; chemicals, reagents, chemical preparations, and chemical substances all for use in analysis and testing in laboratories for medical purposes; chemical reagent for medical purposes, namely, a bioconjugation system for conjugation and immobilization of peptides, fluorophores, biomolecules, proteins, carbohydrates, DNA/RNA or other molecules; chemicals and chemical reagents for medical use; reagents in kit form for performing assays for medical purposes; chemicals agents for use in separation for medical use; cell culture media for use in separation techniques for medical use; biochemical test kits comprising assays and reagents for medical purposes; assay kits comprising assays and reagents for measuring, monitoring, testing and analyzing proteolytic activation and antibody binding for medical purposes; conjugation and labelling kits comprised primarily of reagents, antibodies, labelling enzymes and chemicals for performing conjugation and labelling reactions for medical purposes; reagents in kit form for performing assays for medical purposes; flow cytometry kits comprised of research reagents, buffers and controls for medical purposes. (1) Treatment of materials by chemical processes; purifying proteins; custom production of bioconjugates, biopolymers, and conjugation reagents for others; custom manufacturing services of goods relating to conjugate blocking technology; custom manufacturing services of goods relating to assays and lateral flow assays and enhancement and performance of lateral flow assays.
(2) Chemical analysis and research for others; medical laboratory services; testing services for medical and scientific research purposes; research and development services for others in the fields of nucleic acid, protein and antibody conjugation and modification; testing, research, design and development services in the field of biochemicals for scientific and medical research purposes; scientific and technological services, namely, custom conjugation, lateral flow assay development, lateral flow assay creation, and research and design relating to conjugate blocking technology; custom design and formulation in the nature of research, development, product evaluation of chemicals, assays and reagents for separation and purification techniques; testing, research, design and development services in the field of biochemicals for scientific and medical research purposes; providing technical support and consultation services relating to antibody selection and use, namely, providing technical scientific research consultation services.
A fluorescence detection system, including apparatus and methods, suitable for qPCR and other fluorescence-based analyses. The system may comprise various components, including a stage, an illumination module, a detection module, and an optical relay structure. The stage may be configured to support a sample holder. The illumination module may include one or more discrete light sources configured to produce excitation light. The detection module may be configured to detect fluorescence emission light produced, in response to the excitation light, by a fluorescent sample positioned in the sample holder. The optical relay structure may include a beamsplitter assembly configured to direct the excitation light from the illumination module along an illumination path to the sample holder and to direct the fluorescence emission light from the sample holder along a response path to the imaging module. The system may enhance the quality of excitation light hitting samples in the sample holder.
G01N 21/71 - Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light thermally excited
98.
MIXED MODE CATIONIC EXCHANGE CHROMATOGRAPHY LIGANDS BASED ON SUBSTITUTED 2-BENZAMIDO ACETIC ACID STRUCTURES
The subject invention pertains to mixed mode chromatography ligands and chromatography matrices suitable for the purification of proteins from biological sources or biological samples. Methods of making chromatography matrices comprising the disclosed ligands and using the disclosed chromatography matrices are also provided.
C07K 1/16 - Extraction; Separation; Purification by chromatography
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
This disclosure pertains to a consumable product (microplate) suitable for use in assays utilizing single-molecule recognition through equilibrium Poisson sampling (SiMREPS) and in other assays employing total internal reflection fluorescence (TIRF) or HiLo microscopy. The disclosed microplate is also suitable for use in other high throughput assay systems, such as single-molecule FRET, ligand-receptor binding studies, membrane biology assays, cell-based TIRF and near- TIRF assays. The disclosure further pertains to the use of the microfluidic microplate for the detection of analytes, including nucleic acids, polypeptides, carbohydrates, lipids, post-translational modifications, amino acids, metabolites, and small molecules.
Tagging biological substrates with molecular barcodes in partitions can provide novel biological insight of the substrates that co-localize to discrete partitions, through the sequencing of the molecular barcodes and analysis, thereof. Methods and compositions for determining the proximity of two barcoding oligonucleotides (e.g., in a single partition or adjacent on a tissue section) using a determination of the presence of a 9 bp sequence resulting from tagmentation in different nucleic acid fragments linked to different barcoding oligonucleotides is provided.
