THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM (USA)
Inventor
Fujise, Ken
Pinkaew, Decha
Blagg, Brian
Serwetnyk, Michael
Brackett, Christopher M.
Zuo, Ang
Halim, Hasseri
Abstract
Described herein is the development of ureas and thioureas as small molecular weight fortilin inhibitors. Small molecular weight compounds that inhibit fortilin, as well as compositions, and methods for using same, described herein ameliorate atherosclerosis by targeting fortilin, not hypercholesterolemia or lipid metabolism. This contrasts with the majority of anti-atherosclerosis therapies that rely on lipid lowering and modification. The data presented herein show that the inhibition of fortilin leads to the polarization of macrophages to the anti-inflammatory, anti-atherosclerotic, M2 phenotype. These inhibitors can be used to treat atherosclerosis as well as fortilin-associated cancers.
A61K 41/00 - Medicinal preparations obtained by treating materials with wave energy or particle radiation
A61K 31/175 - Amides, e.g. hydroxamic acids having the group N—C(O)—N or N—C(S)—N, e.g. urea, thiourea, carmustine having the group , N—C(O)—N=N— or , e.g. carbonohydrazides, carbazones, semicarbazides, semicarbazones; Thioanalogues thereof
2.
MICRO PUNCTURE SYSTEM FOR TRANSJUGULAR INTRAHEPATIC PORTOSYSTEMIC SHUNT
The present disclosure provides a device comprising a catheter having a first end and a second end opposite the first end. The catheter includes a first lumen, a second lumen, and a third lumen. The first lumen is configured to receive a guidewire therethrough to position the device in a. desired anatomy. Tire device also includes a needle movably positioned within the second lumen of the catheter. The needle includes a first end and a second end opposite the first end, and the needle is hollow such that the needle includes a lumen extending from the first end to the second end. The needle is configured to transition from a first position in which the first end of the needle is positioned entirely within the second, lumen of the catheter to a second position in which the first end of the needle extends out of the second lumen of the catheter. Tire needle has a. non-zero radius of curvature in the second position. The device also includes a balloon fixed to the first end of the catheter. The balloon is in fluid communication with the third lumen. The device also includes a handle coupled to the second end of the catheter.
T. pallidumT. pallidumT. pallidumT. pallidum is present in the sample. The disclosed elements can be used for clinical tests to determine whether the patient has syphilis as the basis for further clinical testing or treatment of syphilis.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Embodiments of the present disclosure provide compositions and methods for injectable recombinant protein-based hydrogels for therapeutic delivery. Embodiments of the composition and methods comprise a recombinant protein, a cell consortia, and a biocompatible medium. The recombinant protein comprises one or more self-association domains and a flexible linker comprising a pseudorepeat of amino acids, forming stable hydrogels in contact with the cell consortia. Such composition and methods have broad application in delivery of viable cells to a biological tissue for tissue repair and regeneration.
A61K 38/16 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
A61K 9/00 - Medicinal preparations characterised by special physical form
In some embodiments, a computer-implemented method of enhancing sequence read data from a cell-free DNA (cfDNA) sample from a subject for predicting a pregnancy-related condition is provided. A computing system determines a coverage profile based on sequence read data for a plurality of informative sites associated with specific tissue types, cell types, or cell states. The computing system generates a prediction of a presence of or an absence of the pregnancy-related condition by providing at least features from a set of features based on a predicted fetal fraction and a set of features based on the coverage profile as input to at least one machine learning model trained to predict a probability of future onset of the pregnancy-related condition based on the features. In some embodiments, a computer-implemented method of enhancing sequence read data for predicting fetal fraction is provided.
G16B 20/00 - ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
G16B 20/20 - Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
G16B 40/00 - ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
6.
POLYMER-BASED NANOPLATFORM FOR MRNA DELIVERY TO MULTIPLE CANCER CELL TYPES AND HUMAN INDUCED PLURIPOTENT STEM CELLS
A nanoparticle for delivery of mRNA to cell, comprising a core comprising a polyethylenimine polymer having fluorinated groups covalently coupled thereto and with mRNA reversibly associated therewith, and a shell surrounding the core comprising heparin. Pharmaceutical compositions that include the nanoparticle and methods for using the nanoparticle for transfecting cells are provided.
A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
C12N 15/11 - DNA or RNA fragments; Modified forms thereof
7.
TH2 VACCINE-BASED PREVENTION AND TREATMENT OF INFLAMMATION IN OBESITY
Overexpressed proteins associated with inflammatory adipocytes have been demonstrated in TNF-a inflamed adipocyte lines derived from humans and mice as well as in visceral fat derived from mice fed an inflammatory-generating high fat high sucrose diet. The adipocyte-associated proteins are immunogenic in humans and mice, and can be used as a vaccine that drives Type II T-cells to inflammatory adipose tissue. Compositions and methods for the prevention and treatment of disease associated with metabolic obesity, including cancer, such as breast cancer, and non-alcoholic fatty liver disease (NAFLD) are provided.
C07K 14/47 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from humans from vertebrates from mammals
A61K 39/00 - Medicinal preparations containing antigens or antibodies
8.
SYSTEM AND METHOD TO DIRECT THE DIFFERENTIATION OF PLURIPOTENT STEM CELL-DERIVED ODONTOBLASTS
The technology as described herein relates to discovery of methods for generating odontoblasts or organoids thereof in vitro and compositions that utilize the generated odontoblasts for restoringtooth structure.
ASOCIACIÓN CENTRO DE INVESTIGACIÓN COOPERATIVA EN BIOMATERIALES - CIC BIOMAGUNE (Spain)
UNIVERSITY OF THE BASQUE COUNTRY, UPV/EHU (Spain)
Inventor
Nelson, Alshakim
Sánchez Rexach, Eva
Sadaba, Naroa
Yu, Siwei
Sardón Muguruza, Haritz
De Aberasturi, Dorleta Jiménez
Liz-Marzan, Luis Manuel
Abstract
Shape-restoring materials, as well as techniques for generating shape-restoring materials, are described. An example method includes generating a construct by exposing, to UV-visible light, a resin comprising a globular protein, a water-soluble co-monomer, light-to-heat converting nanoparticles, water, and a photoinitiator. At least a portion of the water is removed from the construct. The construct is converted from a first shape to a second shape by applying a force to the construct. The construct is reverted to the first shape in response to being exposed to NIR light, due to the absorption of the NIR light by the nanoparticles.
A ram accelerator for accelerating a projectile is provided. The ram accelerator includes a first tube body having a first projectile bore, a second tube body having a second projectile bore axially aligned with the first projectile bore, and a baffle positioned between and operably coupling the first and second tube bodies. The baffle can have an annular baffle wall defining a central bore that is axially aligned with the first and second projectile bores, and a chamber arranged adjacent to the annular baffle wall. The chamber can extend radially outward from the central bore and the annular baffle wall can be configured to sweep a combustion wave relative to the projectile to prevent the combustion wave from traveling ahead of the projectile, leading to an unstart mechanism of the projectile in the ram accelerator.
F41A 1/02 - Hypervelocity missile propulsion using successive means for increasing the propulsive force, e.g. using successively initiated propellant charges arranged along the barrel length; Multistage missile propulsion
11.
COMPOSITIONS INCLUDING ENDOPHYTES FOR IMPROVING PLANT NUTRITION, GROWTH, AND PERFORMANCE AND METHODS OF USING THE SAME
Endophyte inoculant compositions, methods of making such compositions, methods of using such compositions, and physiologically altered plants treating with such compositions are disclosed. The endophyte inoculant composition may include one or more of endophyte strains WW5, WW6, WW7, and PTD1, which promote plant mineral nutrient acquisition and uptake, vigor, health, growth, and yield when applied to non-native host plants.
Disclosed herein is a generalizable and intuitive strategy to photomodulate biomaterials in full 3D non-discrete patterns across cellular scales through grayscale image z-stack-guided multiphoton optical-lithography (GIZMO). In one aspect a method for optical patterning of a material is presented with the method comprising of initializing a laser, wherein the laser is focused through an objective that is movable in the x, y, and z directions; wherein a laser intensity is modulated while in motion or progress throughout a raster scanning.
Systems, devices, kits, and methods for generating sequencing libraries for detecting low-frequency variants, and for distinguishing low-frequency biological variants from low-frequency technical variants, with duplex sequencing. Methods include introducing multiple levels of indices to cellular nucleic acid molecules of cells of a biological sample to enable differentiation of a cell from other cells of the sample, differentiation of a double-stranded cellular nucleic acid molecule from others of the cell, and differentiation of the individual strands of the double-stranded cellular nucleic acid molecule. Technical variants introduced during the workflow are identified as such due to their occurrence in only one family of duplex strands of the sequencing library, and biological variants are detected due to their occurrence in both families of duplex strands of the sequencing library. Detected biological variants can be linked with a particular cell of the biological sample in a high-throughput manner.
A method includes accessing training images and labels, where the training images depict fluorescence from samples and the labels indicate pH levels of the samples. The method also includes training a computational model, using the training images and the labels, to determine pH levels on tissue inside of a patient's mouth depicted by captured images. Another method includes generating one or more images of tissue inside of a patient's mouth, where the one or more images depict fluorescence from the tissue. The method also includes determining a pH level on the tissue using a model and pixel intensities of the one or more images, and generating an indication of the pH level on the tissue.
G06T 7/73 - Determining position or orientation of objects or cameras using feature-based methods
16.
BIO-IMPEDANCE SENSING FOR GESTURE INPUT, OBJECT RECOGNITION, INTERACTION WITH PASSIVE USER INTERFACES, AND/OR USER IDENTIFICAITON AND/OR AUTHENTICATION
This disclosure describes systems, apparatuses, and methods that utilize electric field sensing in an antenna topology. In some embodiments, the systems, apparatuses, and methods use a sensing modality, such as bio-impedance sensing, to detect and/or determine one or more user activities. The bio-impedance sensing can be used for held-object or touched- object recognition, gesture input recognition (e g., recognition of one-handed gestures, two- handed gestures, etc.), user interface (UI) interaction by utilizing electrically passive components, and/or biometric identification and/or authentication.
A61B 5/0507 - Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves using microwaves or terahertz waves
A61B 5/11 - Measuring movement of the entire body or parts thereof, e.g. head or hand tremor or mobility of a limb
G06F 3/046 - Digitisers, e.g. for touch screens or touch pads, characterised by the transducing means by electromagnetic means
G01S 13/89 - Radar or analogous systems, specially adapted for specific applications for mapping or imaging
A61B 5/053 - Measuring electrical impedance or conductance of a portion of the body
A method includes irradiating, via a heating element, a first portion of a workpiece with electromagnetic radiation having an oscillation frequency within a range of 1 MHz to 300 MHz, thereby heating the first portion of the workpiece. The method also includes applying a pressure to the first portion of the workpiece via an actuator and making a determination that a condition of the first portion or the actuator satisfies one or more criteria. The method also includes moving the workpiece and/or the actuator, in response to making the determination, such that a second portion of the workpiece is aligned with the actuator. A system includes one or more processors, an infrared camera, an actuator, a platform, a motor, a power source, a load cell, and a computer readable medium storing instructions that, when executed by the one or more processors, cause the system to perform the method.
B29C 65/04 - Dielectric heating, e.g. high-frequency welding
B29C 65/14 - Joining of preformed parts; Apparatus therefor by heating, with or without pressure using wave energy or particle radiation
B29C 65/34 - Joining of preformed parts; Apparatus therefor by heating, with or without pressure using heated elements which remain in the joint, e.g. "verlorenes Schweisselement"
18.
USING ADAPTIVE SEQUENCING AND HARDWARE-ACCELERATED STORAGE TO ACCELERATE METAGENOMIC SAMPLE ANALYSIS
In some embodiments, a computer-implemented method of predicting a molecule type using segment read information is provided. A computing device receives a segment read for a molecule. The computing device determines one or more k-mers for the segment read. For each k-mer of the one or more k-mers, the computing device retrieves one or more records from a hash table using the k-mer as a key, wherein each record includes an identifier associated with a molecule type. The computing device generates a molecule type prediction for the segment read based on the identifiers of the retrieved one or more records.
G16B 50/00 - ICT programming tools or database systems specially adapted for bioinformatics
G16B 50/30 - Data warehousing; Computing architectures
G16B 40/00 - ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G06F 16/13 - File access structures, e.g. distributed indices
G06F 16/00 - Information retrieval; Database structures therefor; File system structures therefor
19.
BONDING OF STRUCTURES USING HIGH INTENSITY FOCUSED ULTRASOUND (HIFU)
Methods and systems for bonding of structures using high intensity focused ultrasound are disclosed. In one embodiment, a method for assembling components using ultrasound includes: positioning a first part of an assembly with respect to a second part of the assembly; heating a region of the assembly by focusing the ultrasound from an ultrasound transducer to the region of the assembly; and, in response to heating the region of the assembly, bonding the first part of the assembly to the second part of the assembly.
A diagnostic system for analyzing an analyte is described. In an embodiment the diagnostic system includes a fluidic single-use disposable and a base cooperatively coupleable thereto. In an embodiment, the fluidic single-use disposable comprises a sample processing subcomponent configured to receive a sample and emit signal light if the sample comprises an analyte; a fluidic single-use disposable housing encompassing the sample processing subcomponent, the fluidic single-use disposable housing comprising: a first major side; and a fluidic single-use disposable viewing window disposed in the first major side and positioned to allow light emitted from the sample processing subcomponent to pass through the fluidic single-use disposable viewing window. In an embodiment, the base comprises a base housing comprising: a first major side shaped to cooperatively couple with the first major side of the fluidic single-use disposable housing.
G01N 21/29 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G01N 21/27 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
In some embodiments, a method of controlling a follower vehicle in a vehicle platoon is provided. A follower vehicle controller in the follower vehicle determines a centralized control command based on centralized control information. The follower vehicle controller determines a local control command based on local sensing information using a delayed self reinforcement (DSR) technique. The follower vehicle controller applies weights to the centralized control command and the local control command. The follower vehicle controller combines the weighted centralized control command and the weighted local control command to create a combined control command. The follower vehicle controller uses the combined control command to control a speed of the corresponding follower vehicle.
G01S 19/40 - Correcting position, velocity or attitude
B60W 30/16 - Control of distance between vehicles, e.g. keeping a distance to preceding vehicle
H04W 4/46 - Services specially adapted for particular environments, situations or purposes for vehicles, e.g. vehicle-to-pedestrians [V2P] for vehicle-to-vehicle communication [V2V]
G05D 1/02 - Control of position or course in two dimensions
G01S 13/93 - Radar or analogous systems, specially adapted for specific applications for anti-collision purposes
22.
PROTEIN-DERIVED PEPTIDE MULTIPLEXES FOR BIOMIMETIC DETECTION OF VOC PROFILES
The present disclosure provides peptides, chimeric molecular constructs, and compositions thereof, that can bind volatile organic compounds (VOCs) and be utilized to distinguish VOC profiles, e.g., indicative of disease, as well as related methods. A VOC-based gas sensing approach described in the present specification can be an effective screening tool, due, at least in part, to its speed, ease of use, sensitivity, specificity, and because it does not rely on binding to specific nucleic acid fragments or proteins to function.
Use of the epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), mavelertinib, as a treatment for giardiasis in humans, veterinary animals, and livestock is described. Mavelertinib demonstrates a high selectivity index for Giardia compared to mammalian cells and therefore provides an effective and safe treatment for giardiasis. Therapeutically effective amounts for oral administration are also described.
The present disclosure provides methods for fabricating an instrumented prosthetic socket including a. layered, fabrication with inductive sensors positioned between electrically insulating layers of the socket. The present disclosure further provides methods for tracking of position relationship between socket and socket liner using inductive sensor arrays, and inductive sensor configurations for placement on a curved surface.
Various techniques for generating nanocellulose are described. An example method includes generating a first material by performing alkaline peroxide pulping on heterogenous biomass; generating a second material by removing at least a portion of a dissolved lignin fraction from the first material; and generating a third material by performing mechanical fibrillation on the second material. A fourth material is generated by performing an oxidation pretreatment on the third material. A fifth material is generated by performing mechanical fibrillation on the fourth material. The fifth material includes lignocellulosic microfibrils (LCMF) having a dimension in a range of about 10 to about 1,000 nanometers (nm) and/or lignocellulosic nanofibrils (LCNF) having a dimension in a range of about 1 to about 10 nm.
An example method includes identifying a first number of photons detected by a detector during a first time and when the detector is disposed at a first location and/or first rotation. The example method further includes identifying a second number of photons detected by the detector during a second time and when the detector is disposed at a second location and/or second rotation. In addition, the example method includes determining a value of a pixel or voxel of an image corresponding to a region of a field-of-view (FOV) based on the first number of photons, the first location and/or the first rotation, the second number of photons, and the second location and/or the second rotation.
A radioisotope target station, including a target housing. The target housing includes a target material, and a target backing material. The radioisotope target station includes a loader mechanism comprising at least one jaw configured to secure the target housing, a loading position comprising a magazine, wherein the magazine is configured to hold two or more target housings, an irradiation position comprising a beam, where the beam is configured to irradiate the target housing orthogonally, a cooling fluid source fluidly coupled to the loader mechanism, where the cooling fluid source is configured to cool the target housing as the target housing is irradiated, and an ejection position comprising an ejector and an ejection chute, where the ejector is configured to detach the target housing from the loader mechanism and the ejection chute is configured to receive the target housing when detached.
G21K 5/08 - Holders for targets or for objects to be irradiated
G21G 1/00 - Arrangements for converting chemical elements by electromagnetic radiation, corpuscular radiation, or particle bombardment, e.g. producing radioactive isotopes
H05K 7/20 - Modifications to facilitate cooling, ventilating, or heating
H05H 6/00 - Targets for producing nuclear reactions
H05H 7/00 - PLASMA TECHNIQUE; PRODUCTION OF ACCELERATED ELECTRICALLY- CHARGED PARTICLES OR OF NEUTRONS; PRODUCTION OR ACCELERATION OF NEUTRAL MOLECULAR OR ATOMIC BEAMS - Details of devices of the types covered by groups
G21G 1/04 - Arrangements for converting chemical elements by electromagnetic radiation, corpuscular radiation, or particle bombardment, e.g. producing radioactive isotopes outside of nuclear reactors or particle accelerators
A method includes detecting an acceleration of an object with an accelerometer, capturing a first image of an environment of the object at a first time and a second image of the environment at a second time that is after the first time, determining a state of the object based on the acceleration and one or more of the first image or the second image, the state including one or more components that include an orientation, an angular velocity, a position, or a translational velocity of the object, and performing an action based on the state of the object.
Microfluidic systems, kits, and methods for characterization of polycystic kidney disease (PKD) are described. In an embodiment, the microfluidic system includes a flow device comprising an inlet, an outlet, and a channel comprising a functionalized site configured for cell culture. In an embodiment, a genetically modified (GM) human kidney organoid is cultured at the functionalized site, optionally in the presence of a fluidic flow, to produce PKD cysts for use as a model system for characterization of mechanisms of PKD onset, progression, diagnosis, and response to treatment.
Methods and systems to reduce symptoms of cerebral palsy in children are disclosed herein. In one embodiment, a method for alleviating spasticity of a lower extremity caused by a brain injury includes attaching at least one electrode on a surface of patient's skin, proximate to patient's spinal cord. The method also includes subjecting the patient to a regimen of training that improves walking of the patient; applying a series of pulses to the at least one electrode while the patient is in training; and in response to applying the series of pulses to the at least one electrode, inducing stimulation in the spinal cord that alleviates spasticity of the lower extremity.
The disclosure features compositions and methods for the treatment of trinucleotide repeat expansion disorders. The compositions described herein that may be used to treat such disorders include at least one nucleic acid construct comprising a first nucleic acid sequence. In some embodiments, the first nucleic acid sequence encodes a therapeutic protein. In some embodiments, the first nucleic acid sequence encodes a MBNL protein. In some embodiments, the first nucleic acid sequence encodes MBNL1 protein. The composition may comprise at least one nucleic acid construct comprising a second nucleic acid. In some embodiments, the second nucleic acid sequence encodes an interfering RNA construct that suppresses the expression of RNA transcripts containing aberrantly expanded repeat regions. Disclosed herein are also methods of increasing the presence of functional muscleblind-like protein (MBNL) in the nucleus of a cell with expression control in tissue types and methods of treating muscular dystrophy or spliceopathy using the compositions disclosed herein.
C07K 14/47 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from humans from vertebrates from mammals
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 21/00 - Drugs for disorders of the muscular or neuromuscular system
C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
A ram accelerator for accelerating a projectile is provided. The ram accelerator includes a baffle section configured to start the projectile in the ram accelerator without an obturator. The ram accelerator generally includes a tube having a projectile bore; and a baffle section operably coupled to a proximal end of the tube. The baffle section has an annular baffle wall defining a central bore axially aligned with the projectile bore; and a propellant chamber arranged adjacent to the annular baffle wall, wherein the propellant chamber is configured to enclose a propellant. The propellant can be ignited as the projectile passes through the baffle section by pressure created by the projectile, by an ignition source, or by a flame carried by the projectile to start the ram acceleration of the projectile.
33.
SYSTEMS AND METHODS INCLUDING COMBINATORIAL OPTIMIZATION CIRCUITRY UTILIZING SRAM CELLS AND NOISE
Embodiments of the present disclosure provide systems, devices, and methods for solving combinatorial optimization problems using a device that functions as a CMOS Ising machine. An example device includes circuitry for linear operation, circuitry for noise addition, and circuitry for nonlinear operation. The circuitry for linear operation includes a plurality of SRAM cells in an SRAM cell array that store values of a coupling matrix. In some embodiments, circuitry for linear operation, circuitry for noise addition, and circuitry for nonlinear operation may be fabricated on a semiconductor substrate. In some embodiments, the device may include a feedback loop including circuitry for linear operation, circuitry for noise addition, and circuitry for nonlinear operation, wherein the circuitry for linear operation is coupled to the circuitry for nonlinear operation.
G06F 17/17 - Function evaluation by approximation methods, e.g. interpolation or extrapolation, smoothing or least mean square method
G06N 10/20 - Models of quantum computing, e.g. quantum circuits or universal quantum computers
G06N 10/80 - Quantum programming, e.g. interfaces, languages or software-development kits for creating or handling programs capable of running on quantum computers; Platforms for simulating or accessing quantum computers, e.g. cloud-based quantum computing
34.
SYSTEMS AND METHODS OF PHENOTYPE CLASSIFICATION USING SHOTGUN ANALYSIS OF NANOPORE SIGNALS
A computer-implemented method of phenotype classification is provided. A computing system receives a plurality of segmented events generated by a plurality of nanopores in response to a sample being applied to the plurality of nanopores, wherein each segmented event of the plurality of segmented events represents ionic current changes during a protein interaction with a nanopore of the plurality of nanopores. The computing system processes the plurality of segmented events to create at least one set of model input data. The computing system provides the at least one set of model input data as input to at least one classifier model to generate a classification of the sample. The computing system transmits the classification for presentation on a display device.
G01N 33/483 - Physical analysis of biological material
G01N 27/42 - Measuring deposition or liberation of materials from an electrolyte; Coulometry, i.e. measuring coulomb-equivalent of material in an electrolyte
Synthetic genetic platforms in eukaryote cells (such as yeast) is described. A representative synthetic genetic platform includes a eukaryote cell genetically modified to express an auxin receptor, an auxin response factor, and a reporter, as well as a fusion construct including a Lis1 Homology (LisH) domain fused to an auxin-responsive protein. The synthetic genetic platforms can be used, for instance, to understand developmental and pathological LisH domain variants, and to test bioactive molecules for LisH domain activity.
A method of fabricating a wearable and elastically stretchable thermoelectric generator or other thermoelectric device can involve arranging thermoelectric pellets and printing a thermal insulation material alongside and/or around the thermoelectric pellets to form a core layer. The core layer can be cured, and a liquid metal can be selectively deposited on the thermoelectric pellets to form contact points. Printing conductive ink can form stretchable electrical interconnects that define connections among the thermoelectric pellets via the contact points to form an initial middle layer that includes the stretchable electrical interconnects. Printing a thermal interface material may form an initial outer layer positioned between the initial outer layer and an initial side of the core layer to form a layered assembly. The layered assembly may be inverted and receive additional printed layers building away from the core layer, such as an additional interconnect layer and an additional thermal interface layer.
Disclosed herein are polypeptides that bind to the epithelial cell adhesion molecule (EpCAM) and programmed death-ligand I (PDL1) receptors, nucleic acids encoding them, and methods for their use in treating or limited development of cancer, autoimmune disease, or inflammation. The disclosure further provides nucleic acids encoding a polypeptide of the disclosure: expression vector comprising a nucleic acid of the disclosure operatively linker to a suitable regulatory control element, host cells comprising a polypeptide, fusion protein, nucleic acid, or expression vector of any embodiment of the disclosure, and pharmaceutical compositions, comprising the polypeptide, fusion protein, nucleic acid, expression vector, or host cell of any embodiment; and a pharmaceutically acceptable carrier.
C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
The invention described here contains a nucleic acid expression cassette comprising a transcriptional regulatory region operably linked to a nucleic acid sequence encoding a FKRP, an RNA transcript comprising a modified 5' and/or 3' untranslated region (UTR) that will be utilized to treat a variety of FKRP-mediated diseases.
C07K 14/47 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from humans from vertebrates from mammals
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
The present disclosure is generally directed to macrocyclic oligoamides useful in the inhibition of HDAC6, and methods for treating diseases that are ameliorated by the inhibition of HDAC6.
Endophyte inoculant compositions, methods of making such compositions, methods of using such compositions, and physiologically altered plants treating with such compositions are disclosed. The endophyte inoculant composition may include one or more of endophyte strains WW5, WW6, WW7, and PTD1, which promote plant mineral nutrient acquisition and uptake, vigor, health, growth, and yield when applied to non-native host plants.
C07D 401/14 - Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
Extendable and flexible drive shaft members and drive shaft assemblies include circumferentially distributed anisotropic mechanisms configured to accommodate extension and flexure. A drive shaft member includes anisotropic mechanisms that are interconnected and distributed circumferentially around a longitudinal centerline of the drive shaft member. Each of the anisotropic mechanisms is configured to accommodate extension of the anisotropic mechanism parallel to the longitudinal centerline. The anisotropic mechanisms combine to transmit a torque along the longitudinal centerline.
Polypeptides having an amino acid sequence at least 50% identical to, and identical at least at one identified interface position, to the amino acid sequence selected from the group consisting of SEQ ID NO: 1 -44, and polypeptides having an amino acid sequence at least 50% identical to the amino acid sequence selected from the group consisting of SEQ ID NO:45- 58, are provided, as well as fusion proteins thereof, nanoparticles thereof, and methods for treating or limiting development of an infection.
SEATTLE CHILDREN'S HOSPITAL D/B/ASEATTLE CHILDREN'S RESEARCH INSTITUTE (USA)
Inventor
Von Saint Andre-Von Arnim, Amelie
Diblasi, Robert, Mariano
Poli, Jonathan, Arthur
Sonaike, Ibukun
Yanay, Ofer
Vamos, Andrew
Abstract
A system includes a container configured to contain a liquid. The system also includes a tubing apparatus having a first port configured for connection to a gas source, a second port configured for connection to a patient interface, and a third port configured for submersion within the liquid. The system also includes an actuator configured to move the third port vertically within the container. A method includes flowing gas into the first port of the tubing apparatus to the patient interface via the second port of the tubing apparatus. The method also includes moving the third port of the tubing apparatus vertically within the container such that an amount of the liquid in the container that is above the third port changes, thereby changing a pressure at the patient interface.
Compositions, nucleic acid molecules and methods for inducing retinal regeneration and reprogramming of Müller glia (MG) into retinal ganglion cells in a subject are described. Developmental retinal ganglion cell (RGC) transcription factors Pou4f2 and Islet1 increase the Ascl1-induced neurogenic capacity of MG. The combination of Ascl1, Pou4f2 and Islet1 stimulates MG to generate bipolar cells and RGC-like neurons. Likewise, the transcription factor Onecut1, which is expressed in developing retinal cells, but not in MG, induces MG to generate RCG-like cells. Additional transcription factors that can be used include Irx2, Irx5, Neurod2, Ebf1, and Tcf3. MG-derived RGCs can exhibit action potentials in vivo, and display chromatin profiles similar to developing RGCs.
Traumatic injuries of the central nervous system and neurogenerative diseases often lead to permanent loss of neural tissue, lifelong disability, morbidity, and devastating effect on life of patients and their families. The present disclosure provides porous chitosan scaffolds and chitosan thin films useful for neural stem cell adhesion, maintenance, and proliferation, and methods for using and fabricating the scaffolds and thin films.
Endophyte inoculant compositions, methods of making such compositions, methods of using such compositions, and physiologically altered plants treating with such compositions are disclosed. The endophyte inoculant composition may include one or more of endophyte strains WW5, WW6, WW7, and PTD1, which promote plant mineral nutrient acquisition and uptake, vigor, health, growth, and yield when applied to non-native host plants.
The present disclosure provides a device comprising a first connector including a first elongated hollow member configured to be positioned at least partially within a lumen of a proximal portion of a tube. The first connector provides a clamping force to the proximal portion of the tube. The device also includes a second connector configured to be removably coupled to the first connector. The second connector including a second elongated hollow member configured to be positioned at least partially within a lumen of a distal portion of the tube. The proximal portion of the tube is separated from the distal portion of the tube when the first connector is disconnected from the second connector, and the lumen of the proximal portion of the tube is fluidically connected with the lumen of the distal portion of the tube when the first connector is removably coupled to the second connector.
Methods of making membranes and membranes that have high urea selectivity. A flat sheet membrane selectively permeable to urea includes a polymer cast from a polymer solution into a thin membrane having a top and bottom surface; and macrovoids or microvoids extending from the top surface to the bottom surface of the membrane that allow the membrane to be selectively permeable to urea.
Embodiments of the disclosure are drawn to apparatuses, systems, and methods for 3D microdissection of samples. A microdissection system includes an imaging system and an extraction system. The imaging system may include a fluorescent microscope, and collects a 3D image of a sample. A region of interest is identified, in the sample based on the 3D image. The extraction system extracts the identified region of interest. The imaging, identification, and/or extraction may be manual, automated, or a combination thereof.
A system for detecting a biological target in a sample, including a sensor comprised of single-walled nanotubes coating a sample substrate, where the sensor includes a sensing electrode and a control electrode, and an analyzer configured to accept the sensor including a controller configured to measure a resistance of the sensing electrode and a resistance of the control electrode and compare the resistance of the sensing electrode to the resistance of the control electrode. Additionally, a method of detecting a biological target with the system including placing a sample into an analyzer, inserting a sensor made of single-walled carbon nanotubes into the analyzer, wherein the sensor includes a sensing electrode and a control electrode, submerging the sensing electrode into the sample, submerging the sensing electrode into a washing solution, measuring the resistance of the sensing electrode and the control electrode, and comparing the resistances.
G01N 27/04 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
B82Y 15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
Provided herein are compositions and methods directed toward the discovery of improved methods for generating deoxyATP (dATP) in cells that can be delivered to a graft site in the heart to enhance cardiac function.
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 21/00 - Drugs for disorders of the muscular or neuromuscular system
53.
SYSTEMS AND METHODS FOR ENERGY STORAGE USING A SOLID-STATE REDOX-TARGETING AQUEOUS ANOLYTE
A redox flow battery is provided that incorporates an aqueous anolyte that includes a negative solid-state material, along with methods of using the battery.
Improved influenza hemagglutinin (HA) antigens that include a stem (HA2) domain and a transmembrane domain to tether the antigen to a cell membrane for improved immunogenicity with vaccination. In embodiments, the tethered, miniature HA antigens include amino acid residue substitutions, additions, and/or deletions for stabler trimerization at the cell membrane and do not include all or part of the HA2 head subdomain. In embodiments, the improved antigens facilitate production of cross-reactive antibodies against the stem domain. In embodiments, the antigens are encoded by RNA of an RNA vaccine and administered to individuals for broad immunogenicity against influenza viruses.
Systems, modules, methods, and machine-readable storage media for Frequency Angular Resolving (FAR) are described. In an embodiment, the system comprises a transmitter and a receiver. In an embodiment, the transmitter comprises a source of electromagnetic radiation; a driver circuit configured to generate a drive signal at an oscillation frequency; an acousto-optical beam steering device optically coupled with the source of electromagnetic radiation and the driver circuit and configured to emit electromagnetic radiation at an emission angle as a function of the oscillation frequency. In an embodiment, the receiver comprises a radiation sensor optically coupled with the source of electromagnetic radiation. In an embodiment, the system comprises an electro-optic modulator optically coupled to the source of electromagnetic radiation, the electro-optic modulator configured to modulate a frequency of light emitted to the acousto-optical beam steering device.
G01S 17/88 - Lidar systems, specially adapted for specific applications
G02B 26/08 - Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
G02F 1/29 - Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulating; Non-linear optics for the control of the position or the direction of light beams, i.e. deflection
Proteins having luciferase activity are disclosed, having the secondary structure arrangement H1-L1-H2-L2-E1-L3-E2-L4-H3-L5-E3-L6-E4-L7-E5-L8-E6, wherein "H" is a helical domain, "L" is a loop domain, and "E" is a beta strand domain; wherein: (a) the H1 domain is at least 18 or 19 amino acids in length; residue 14 of the H1 domain is Y, D, or E, and residue 18 of the H1 domain is D or E: (b) the E3 domain is at least 6, 7, 8, 9, or 10 amino acids in length and residue 2 of the E3 domain is R; and (c) the E5 domain is at least 10, 11, 12, 13, or 14 amino acids in length and residue 9 of the E5 domain is H or M.
Passive grippers can be computationally generated for grasping objects. For example, a method described herein for producing a passive gripper for the object can include identifying a set of grasp configurations for the object. The method can also include selecting, from the set, a particular grasp configuration configured for performing passive engagement and disengagement with the object. Additionally, the method can include generating a template for the passive gripper based on the particular grasp configuration. The method can include fabricating the passive gripper based on the template.
B29C 67/00 - Shaping techniques not covered by groups , or
G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control (DNC), flexible manufacturing systems (FMS), integrated manufacturing systems (IMS), computer integrated manufacturing (CIM)
B29C 64/00 - Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
Cyclic peptides are disclosed that comprise an amino acid sequence having no more than 3 amino acid substitutions compared to the amino acid sequence selected from the group consisting of SEQ ID NO: 1-123 and 125-131 or mirror images thereof.
C07K 7/64 - Cyclic peptides containing only normal peptide links
C07K 7/54 - Cyclic peptides containing at least one abnormal peptide link with at least one abnormal peptide link in the ring
C07K 7/56 - Cyclic peptides containing at least one abnormal peptide link with at least one abnormal peptide link in the ring the cyclisation not occurring through 2,4-diamino-butanoic acid
C07K 7/02 - Linear peptides containing at least one abnormal peptide link
59.
POTENT AND STABLE POLYPEPTIDE ANALOGUES VIA SERINE/THREONINE LIGATION
C07K 7/08 - Linear peptides containing only normal peptide links having 12 to 20 amino acids
A61K 47/64 - Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
Three to four residue non-naturally occurring macrocycle oligoamide comprising a chemotype of 3 or 4 monomer residues selected from the group consisting of a, b, c, d, e, f, g, h, I, j, k, 1, m, n, o, p, q, r, s, t, u, and v monomers as defined in Table 1 are provided, and methods for design of such macrocycle oligoamides
A61K 31/435 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
A61K 31/395 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
A61K 31/4353 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems
61.
OLIGO-MEDIATED DEPOSITION OF FUNCTIONAL MOLECULES FOR SPATIALLY RESOLVED GENOMICS, TRANSCRIPTOMICS, AND PROTEOMICS
Embodiments of the present disclosure provide compositions and methods for increasing nutrient uptake by plants and for effecting soil remediation. Embodiments of the composition and methods comprise a hydrogel bead, a microbial consortia, and a fungi, and can additionally comprise an excipient for administration of the hydrogel bead comprising the microbial consortia and the fungi, one or more seed, water, one or more nutrients, and combinations thereof. Such composition and methods have broad application to reduce fertilizer requirements and use, and to increase plant nutrient access and uptake. The composition and methods have additional application to remediate contaminated mediums such as soil contaminated with chemicals, petroleum, and explosives.
THE USA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES (USA)
Inventor
King, Neil, P.
Ellis, Daniel
Dosey, Anne
Kanekiyo, Masaru
Abstract
nn, wherein X1, X2, X3, X4, and X5 may independently be any amino acid other than proline, and wherein n can be 1-30: and (b) a second domain selected from the group consisting of (i) a polypeptide antigen, and (ii) a polypeptide component of a nanoparticle, nanoparticles including such polypeptides, and uses thereof.
Enamel forms during early development, before the tooth breaks through the gum, and it covers the visible portion of the tooth. During development, epithelial cells within the enamel organ differentiate into ameloblasts, which produce the hardened, high mineral-content enamel. The technology as described herein relates to discovery of methods for generating ameloblasts or organoids thereof in vitro and compositions that utilize the generated ameloblasts for restoring enamel products.
A capacitive sensor including an electrically conductive material, and a single electrode applied with positive potential, wherein the distance between the single electrode and the electrically conductive material determines the spherical radius for a proximity sensing range. A sensing pad comprising four capacitive sensors, wherein the four sensors are arranged in a 2x2 sensor array a capacitance-to-digital chip communicatively coupled to the four sensors and located in the center of the array, and four lobes extending from the capacitance-to digital chip, wherein each lobe physically connects one of the four sensors to the capacitance-to-digital chip in the shape of an X is disclosed.
G01L 1/20 - Measuring force or stress, in general by making use of electrokinetic cells, i.e. liquid-containing cells wherein an electrical potential is produced or varied upon the application of stress
A61B 5/11 - Measuring movement of the entire body or parts thereof, e.g. head or hand tremor or mobility of a limb
D06M 11/74 - Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with carbon or compounds thereof with graphitic acids or their salts
66.
IRON OXIDE NANOPARTICLE-MEDIATED RADIATION DELIVERY FOR TARGETED CANCER TREATMENT
Method for nanoparticle-mediated deposition of radiation (NMDR) and targeted radiation therapies using a biodegradable and bioabsorbable iron oxide nanoparticle with a biocompatible coating that is effective to overcome various extra- and intra-cellular barriers and selectively accumulate in solid and metastatic tumors to improve the energy transfer of conventional radiotherapy
This disclosure relates to methods and systems for imaging biopsy samples. The system for imaging biopsy samples comprises a window and an imaging device. The window comprises an inflexible and optically clear material. The window is configured to compress a sample disposed in a biopsy needle. The imaging device is configured to maintain a constant working distance to the flattened tissue surface while scanning. The imaging device is configured to capture one or more images of the sample through the window.
G02B 21/32 - Micromanipulators structurally combined with microscopes
A61B 1/05 - Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances characterised by the image sensor, e.g. camera, being in the distal end portion
68.
DIFFERENTIATION OF HUMAN TISSUES WITHOUT CILIA FROM PLURIPOTENT STEM CELLS
Embodiments of the present disclosure provide compositions and methods for making a genetically modified pluripotent stem cell, wherein the genetically modified pluripotent stem cell lacks cilia. Embodiments of the present disclosure also provide compositions and methods for using the genetically modified pluripotent stem cell to generate genetically modified organoids, wherein the genetically modified organoids lack cilia.
Dialysis fluid processing systems and methods to process used dialysis fluid and provide detoxified dialysis fluid to a hemodialysis machine. A dialysis fluid processing system includes a filtration module to filter used dialysis fluid and an oxidation module to generate detoxified dialysis fluid via photo-oxidation and/or electro-oxidation processes. A fluid input operably connects the system with the hemodialysis machine for transport of used dialysis fluid from the hemodialysis machine and a fluid output operably connects the system with the hemodialysis machine for transport of detoxified dialysis fluid to the hemodialysis machine for continued use in hemodialysis by the hemodialysis machine.
SEATTLE CHILDREN'S HOSPITAL, d/b/a SEATTLE CHILDREN'S RESEARCH INSTITUTE (USA)
Inventor
Perez, Krystle
Nelson, Len
Robinson, Timothy
Freaney, Avry
Van, Honglam
Olanrewaju, Ayokunle
Valentine, Gregory, Charles
Seibel, Eric, J.
Fan, Eric
Posner, Jonathan, Dov
Sharma, Manuja
Abstract
A sensor paper is infused with bromothymol blue (BTB), a water-soluble polymer, and a non-ionic surfactant. The BTB has a concentration within the water-soluble polymer that is greater than about 2.99 x 10-6 g/mL. A test, kit includes the sensor paper and a plasma separation membrane having an average pore size that decreases from a first side of the plasma separation membrane to a second side of the plasma separation membrane that is opposite the first side and in contact with the sensor paper. A method includes capturing an image of the sensor paper that is infused with a fluid, determining a potential of hydrogen (pH) of the fluid using the image, and generating an output indicating the pH of the fluid.
A61F 13/42 - Absorbent pads, e.g. sanitary towels, swabs or tampons for external or internal application to the body; Supporting or fastening means therefor; Tampon applicators with wetness indicator or alarm
G01N 31/22 - Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroups; Apparatus specially adapted for such methods using chemical indicators
This disclosure describes examples of systems, apparatuses, and methods for performing wireless communication using thermal noise of one or more components of the systems. For example, thermal noise generated by a resistor or any electronic component with some resistance or impedance may be modulated in order to wirelessly transmit data. An example method includes controlling a switch between a first state of the switch to couple an electronic component to an antenna and present a first impedance to the antenna and a second state of the switch to present a second impedance to the antenna. The first impedance presented to the antenna is a closer impedance match than the second impedance presented to the antenna. The controlling is performed in accordance with a subcarrier frequency and data to modulate thermal noise of a transmitter including the at least one electronic component to transmit a signal including the data.
G06K 19/07 - Record carriers with conductive marks, printed circuits or semiconductor circuit elements, e.g. credit or identity cards with integrated circuit chips
H01Q 1/24 - Supports; Mounting means by structural association with other equipment or articles with receiving set
H04B 5/00 - Near-field transmission systems, e.g. inductive loop type
A method includes capturing, via a camera that is inserted into a cavity, a first image of a surface defining the cavity and compressing the first image, using a compression algorithm, to generate a first feature vector. The method also includes identifying a second feature vector of a plurality of second feature vectors that best matches the first feature vector. The plurality of second feature vectors was generated by compressing second images of the surface using the compression algorithm. The second images were captured prior to insertion of the camera into the cavity and prior to capturing the first image. The method also includes generating, using the second feature vector, output indicating a position and/or an orientation of the camera as the camera captured the first image.
G06T 7/73 - Determining position or orientation of objects or cameras using feature-based methods
G16H 30/40 - ICT specially adapted for the handling or processing of medical images for processing medical images, e.g. editing
A61B 1/307 - Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for the urinary organs, e.g. urethroscopes, cystoscopes
Systems and methods for digital affinity-based detection assays are described. In an embodiment, the method comprises associating an analyte, such as a single-molecule analyte, in a sample with a detectable agent; flowing the sample including the analyte associated with the detectable agent through a flow channel; outputting excitation light through an interrogation window through a portion of the flow channel; and generating an emission signal with a photodetector based on emission light received from the portion of the flow channel.
G01N 35/08 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
A61K 31/395 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
A61K 31/438 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom the ring being spiro-condensed with carbocyclic or heterocyclic ring systems
A61K 31/55 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
75.
GENERATING AND UTILIZING ADULT HEPATOCYTE ORGANOIDS
Techniques for generating and utilizing adult hepatocyte organoids are described. An example method includes generating an organoid by culturing hepatocytes obtained from an adult donor in a hepatocyte culture medium. The hepatocyte culture medium includes a basal medium and 5 µM of a TGF-β inhibitor.
Embodiments of the present disclosure provide systems and methods directed to directional audio source separation. An example method comprising: receiving a plurality of input signals by a plurality of microphones; generating a plurality of beamformed signals based on the plurality of input signals; providing the plurality of beamformed signals and the plurality of input signals to a neural network, wherein the neural network is trained to generate directional signals based on sample beamformed signals and sample input signals; generating an output directional signal using the neural network based on the plurality of input signals and the plurality of beamformed signals; and providing the directional signal to a speaker.
G01S 3/808 - Systems for determining direction or deviation from predetermined direction using transducers spaced apart and measuring phase or time difference between signals therefrom, i.e. path-difference systems
G10L 21/028 - Voice signal separating using properties of sound source
G10L 21/0216 - Noise filtering characterised by the method used for estimating noise
77.
NECK MASSAGING SYSTEMS AND METHODS OF USING THE SAME
An example neck massaging system includes a housing. The housing is configured to fit around a neck of a wearer. As such, the housing includes an interior region defining an opening configured to receive the neck. The housing also includes a top region and a bottom region opposite the top region. The neck massaging system further includes at least one massager attached to the housing. The massager is positioned on the housing to be adjacent to the interior region which allows the massager to contact the neck. In an example, the massager is positioned on the housing such that the massager contacts the internal jugular vein. The neck massaging system also include at least one actuator operably coupled to the massager. The actuator is configured to move a portion of the massager adjacent to the neck in a direction that generally extends from the top region to the bottom region.
Systems and methods for culture of human kidney organoids as a model system for characterizing kidney pathologies and implementing therapeutics for conditions that affect the kidney. A model system for characterization of a pathophysiology includes a culture of human kidney organoids and an organoid culture medium. The organoids may include one or more genome edits as part of an approach for studying genetic factors involved with a pathological process. The culture may include one or more other factors, such as pathological agents and/or anti-pathological agents, for development and evaluation of therapeutics. The approaches may be used for implementation of compositions and methods for virally transducing the kidney or a subset of cells or cell types thereof.
Materials and methods for improving cardiac regeneration are provided, which are based on the surprising discovery that KDM4D synergistically induces cardiac myocyte proliferation with the Hippo-Yap1 pathways. Described is an expression vector that comprises (a) a nucleic acid sequence encoding KDM4D; (b) a nucleic acid sequence encoding an activator/effector of the Hippo-Yap pathway; (c) a promoter that effects overexpression of KDM4D and the activator/effector of the Hippo-Yap pathway; and (d) a regulatory element that inducibly represses the overexpression of KDM4D and/or the activator/effector of the Hippo-Yap pathway. The promoter, which in some embodiments is a tissue-specific promoter, is operably linked to the nucleic acid sequence of (a) and (b). This unexpected discovery of synergistic mechanisms enables new strategies for cardiac regeneration in treating heart disease.
Embodiments of the present disclosure provide an antibody comprising a light chain variable domain and a heavy chain variable domain. The light chain variable domain comprises a CDRL1 with an amino acid sequence as set forth in SEQ ID NO:8; a CDRL2 with an amino acid sequence as set forth in SEQ ID NO:10; and a CDRL3 with an amino acid sequence as set forth in SEQ ID NO:12. The heavy chain variable domain comprises a CDRH1 with an amino acid sequence as set forth in SEQ ID NO:4; a CDRH2 with an amino acid sequence as set forth in SEQ ID NO:16; and a CDRH3 with an amino acid sequence as set forth in SEQ ID NO:18; wherein the antibody specifically binds glypican-3 (GPC3).
C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
Described herein is a generalizable strategy to rapidly and irreversibly activate protein function with full spatiotemporal control. Through development of an exogenously triggerable self-assembling protein construct, bioactive proteins can be stably re-assembled from non-functional split fragment pairs following exposure to a stimulus (e.g., light).
C07K 14/315 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
In some embodiments, a method of controlling speed of an ego vehicle in a vehicle platoon is provided. Cooperative adaptive cruise control (CACC) commands based on at least one of a vehicle-to-vehicle communication control received from a preceding vehicle and a feedback control based on a sensor output of a long-range sensor of the ego vehicle are provided to a speed controller. In response to detecting an occluded state, a minimum spacing value and a minimum relative velocity value between the ego vehicle and the preceding vehicle are determined based on information received before the detection of the occluded state; a safety speed based on the minimum spacing value and the minimum relative velocity value is determined; and an occluded adaptive cruise control command is provided to the speed controller to maintain a speed of the ego vehicle that is less than or equal to the safety speed.
G05D 1/02 - Control of position or course in two dimensions
G08G 1/00 - Traffic control systems for road vehicles
B60W 30/165 - Control of distance between vehicles, e.g. keeping a distance to preceding vehicle automatically following the path of a preceding lead vehicle, e.g. "electronic tow-bar"
Embodiments of the present disclosure provide composition and methods for recording an iterative nucleic acid editing event. The compositions and methods described herein comprise a first active target domain, comprising an editable recording sequence configured to hybridize with a first prime editing guide RNA (pegRNA) and one or more inactive truncated target domains comprising a non-editable sequence configured to not hybridize with the pegRNA, wherein the first pegRNA edits the first active target domain, wherein the pegRNA edit shifts the position of the recoding sequence from the editable sequence to the non-editable sequence, thereby changing the editable sequence to a non-editable sequence and the inactive truncated target domain to a second active target domain comprising a second recoding sequence configured to hybridize with a second pegRNA.
Methods for making an intercalated layered film, intercalated layered films, and devices that include the intercalated layered films. In the method, in a first step, a suspension of a dispersed two-dimensional compound in a fluid is filtered through a filtration medium to provide a layered film of the two-dimensional compound on the filtration medium, and in a second step, filtering a solution of an intercalant in a solvent through the layered film of the two-dimensional compound on the filtration medium to provide the intercalated layered film.
Techniques for concentrating analytes in fluid samples are described. An example method performed by a concentrator includes receiving a fluid sample; concentrating an analyte in the fluid sample by extracting water from the fluid sample; and based on concentrating the analyte in the fluid sample, outputting the fluid sample. The analyte is concentrated by extracting the water through a membrane and into a solution including at least one solute at a concentration that is greater than a concentration of at least one solute in the fluid sample, diameters of pores extending through the membrane being shorter than a diameter of the analyte and shorter than a diameter of the at least one solute.
A temperature controlled biological growth surface, including a biomass growth surface configured to support a biomass, and a temperature source in thermal communication with the biomass growth surface. Further, a method for removing one or more of nitrogen, carbon, or phosphate from a medium, including contacting a temperature controlled biological growth surface with the medium, where the biological growth surface comprises an organism configured to remove the one or more of nitrogen, carbon, or phosphate from the medium when within a temperature range, and heating the temperature controlled biological growth surface to within the temperature range in order to allow the organism to remove the one or more of nitrogen, carbon, or phosphate from the medium, or cooling the temperature controlled biological growth surface to within the temperature range in order to allow the organism to remove the one or more of nitrogen, carbon, or phosphate from the medium.
Use of 4-(Propan-2-yl)-N-(pyridin-4-yl)benzamide (C15H16N2O) and similar compounds to treat pain and reduce the aversiveness of negative stimuli is described. The treatments can alleviate pain, anxiety, and depression. Such treatment may reduce the perception of pain or experience of an aversive reaction to a stimulus. The treatments can activate particular portions of the brain region, such as a subpallium or a telencephalic region.
A61K 31/4375 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a six-membered ring having nitrogen as a ring hetero atom, e.g. quinolizines, naphthyridines, berberine, vincamine
A61K 31/395 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
89.
MULTIPLEXED IN SITU SIGNAL AMPLIFICATION WITH SPLINT LIGATION EXTENSION (SLX) PROBES
Embodiments of the present disclosure provide a single stranded nucleic acid construct with a predetermined number of binding sites, wherein the construct comprises a targeting domain, at least one signaling domain, and at least one splint oligonucleotide that hybridizes to complementary regions on the targeting domain and the signaling domain to form a ligation junction to link each domain to form a functional unit. The present disclosure also provides for methods of using the construct to quantify a target nucleic acid through signal intensity. In other embodiments, the disclosure provides use of the construct in multiplexed target detection methods.
Disclosed herein are -sheet polypeptide multimers, comprising two or more monomeric -sheet polypeptides that are covalently linked. In various embodiments, the multimer comprises a dimer, trimer, tetramer, pentamer, or hexamer, or wherein the multimer comprises a dimer. Also disclosed are compositions and medical devices comprising -sheets, and their use for treating and diagnosing amyloid diseases or amyloid-associated diseases.
Provided herein are methods and compositions for delivering large proteins to a subject in need thereof for treatment of a disease or disorder. In certain embodiments, the methods and compositions described herein are useful in the delivery of large proteins to subjects using a protein expression system comprising at first and second AAV vector for the treatment of muscular or neuromuscular disease or disorders.
INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY (ICGEB) (Italy)
Inventor
Murry, Charles E.
Giacca, Mauro
Bortolotti, Francesca
Tsuchida, Hiroshi
Abstract
Described herein are compositions and methods related to enhancing cardiomyocyte transplant engraftment and methods of administering a transplant composition.
Systems and methods for optimizing data-driven decision-making in multi-agent systems are described. The system may construct an equilibrium concept to capture multi-layer and/or k-level depth reasoning by agents. The system may determine best-response type conjectures for agents to interact with one another. In some examples, the system may include a machine or an algorithm interacting with a strategic agent (e.g., a human or an entity). The system may include methods for: (1) data-driven estimation and/or learning of conjectures and associated depth; and (2) data-driven design of algorithmic mechanisms for exploring the conjectural equilibrium (CE) space by influencing the strategic agent(s) behaviors through adaptively adjusting and estimating deployed strategies.
C07K 14/47 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from humans from vertebrates from mammals
A61K 38/16 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
95.
ENZYME-LOADED POLYMERIC NANOPARTICLES AND METHODS OF MANUFACTURE AND USE OF SAME
Enzyme-loaded polymeric nanoparticles for the treatment of disorders such as neurological or non-neurological conditions are described. Using hydrophobic ion pairing, enzymes are loaded into polymeric nanoparticles while retaining enzymatic activity. Surfactants coating the nanoparticle can direct nanoparticles for cellular uptake or for aggregation in the extracellular matrix. Preparing a hydrophobic ion pairing complex for nanoparticle formation.
Embodiments of the present disclosure provide a method for detecting one or more genetic variants in a biological sample. Embodiments of the method include preparing an error-corrected nucleic library for sequencing, wherein the nucleic acid library comprises a double stranded nucleic molecule comprising a hairpin adapter, wherein the hairpin adapter covalently joins each strand of the double stranded nucleic molecule into a single covalently linked duplex strand for self-correction of sequencing errors.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS (USA)
Inventor
Oh, Sewoong
Liu, Xiyang
Mahdavifar, Hessam
Jamali, Mohammad Vahid
Viswanath, Pramod
Makkuva, Ashok
Abstract
In some embodiments, a method of encoding a set of information bits to produce a codeword that encodes the set of information bits for reliable communication is provided. The set of information bits is received. The set of information bits are provided to a plurality of permutation layers separated by neural network processing layers. Each permutation layer accepts an input vector and generates a reordered output vector that is a reordering of the input vector. Each neural network processing layer accepts a vector of input values and generates a vector of output values based on a non-linear function of the vector of input values. The reordered output vector of a final permutation layer of the plurality of permutation layers is provided as the codeword. In some embodiments, a corresponding method of decoding a codeword to retrieve a set of information bits is provided.
A63F 13/30 - Interconnection arrangements between game servers and game devices; Interconnection arrangements between game devices; Interconnection arrangements between game servers
A mechanism of electron-mediated pumping of heat in the absence of charge current is disclosed. The heat pump may be realized in charge-neutral electron systems, such as graphene, coupled to an external pumping potential. Flow of heat in this pumping cycle is not accompanied by voltage buildup along the system, which offers advantages over traditional thermoelectric cooling setups. In a system the heat flux becomes independent of the strength of the pumping potential. For example, for a potential moving with a velocity c the pumping is perfect; the entire heat content of the electron liquid is advected with velocity c. For a general pumping cycle the heat flux is determined by the cycle geometry and disorder strength. Efficiency of heat pumping and magnitude of heat flux corresponds to the case of the hydrodynamic regime for weak disorder.
H02K 3/22 - Windings characterised by the conductor shape, form or construction, e.g. with bar conductors consisting of hollow conductors
H02K 3/24 - Windings characterised by the conductor shape, form or construction, e.g. with bar conductors with channels or ducts for cooling medium between the conductors
100.
CLOSED LOOP COMPOSITE WELDING AND BONDING SYSTEM USING RADIO-FREQUENCY AND PRESSURE
A bonding system includes a heating element, an actuator, an infrared camera, a pressure sensor and/or a displacement sensor, one or more processors, and a computer readable medium storing instructions that, when executed by the one or more processors, cause the bonding system to perform functions including providing a first control signal to a heatmg element, thereby heating a workpiece with electromagnetic radiation having one or more oscillation frequencies within a range of 1 MHz to 2.00 MHz, and providing a second control signal to an actuator, thereby applying a pressure to the workpiece via the actuator. The functions also include detecting a temperature of the workpiece, the pressure applied to the workpiece, and/or a displacement of the actuator, and adjusting the first control signal and/or the second control signal based on the temperature of the workpiece, the pressure applied to the workpiece, or the displacement of the actuator.