The present disclosure provides UV-absorbing polymer dyes and methods for detecting an analyte in a sample by using a binding partner conjugated to a UV- absorbing polymer dye. Compositions comprising UV-absorbing polymer dyes, UV- absorbing tandem dyes, or quenched UV polymer dyes are provided.
A gassing lid assembly enables gas-tight sealing of sample containers in general, also referred to as microplates in some embodiments, with simultaneous guided access for the pipetting unit of a dispensing/pipetting robot, also referred to as a pipettor. The component enables both gas-tight sealing and guided access for the pipetting robot. The gassing lid serves a number of purposes at the same time and provides the following advantages in a non-limiting fashion: a gas tight seal, robot integration without a gassing lid, robot integration with a gassing lid, a sealing mechanism, and anaerobic transport. Reducing the volume above reservoirs of a sample container (e.g., the volume above wells of a microplate) is advantageous in that it reduces the safety risk of high concentrations of gases such as oxygen.
A gassing lid assembly enables gas-tight sealing of sample containers in general, also referred to as microplates in some embodiments, with simultaneous guided access for the pipetting unit of a dispensing/pipetting robot, also referred to as a pipettor. The component enables both gas-tight sealing and guided access for the pipetting robot. The gassing lid serves a number of purposes at the same time and provides the following advantages in a non-limiting fashion: a gas tight seal, robot integration without a gassing lid, robot integration with a gassing lid, a sealing mechanism, and anaerobic transport. Reducing the volume above reservoirs of a sample container (e.g., the volume above wells of a microplate) is advantageous in that it reduces the safety risk of high concentrations of gases such as oxygen.
The disclosure relates to compositions comprising a non-fluorescent component of a polymer dye such as a monomeric component of a polymer dye, a photo-bleached polymer dye, and/or a polymer dye comprising a quenching moiety, for reducing non-specific interactions of polymer dye conjugates, for example, in Flow Cytometric Analysis of a biological sample. Methods for using such compositions, and kits comprising such compositions are also provided.
Compositions, methods, and kits for stable Trypan Blue solutions are provided. In one example, a method includes: cooling a solution of Trypan Blue; and filtering the cooled solution. In another example, a method includes: mixing a water-soluble polymer with a Trypan Blue solution. The method may further include adding one or more ingredients such as an aqueous buffer, an osmolyte, an acid, a base, a buffer, a cell culture medium, water, or combinations thereof.
The disclosure relates to methods and compositions for reducing or eliminating non-specific binding of at least one dye conjugate to cells in a biological sample. A dye conjugate is contacted with at least one zwitterionic or anionic surfactant before, during or after the dye conjugate is contacted with a blood sample, resulting in substantially reduced non-specific binding of the dye conjugate to cells in the biological sample.
The disclosure relates to, among other things, an automated flow cytometric method and system for the analysis and enumeration of at least one of hematopoietic stem cells, hematopoietic progenitor cells, and T-cells.
The disclosure relates to methods and apparatus for processing fluids through the use of a magnetic assembly wherein the magnetic assembly includes at least one fluid chamber containing a fluid and magnetic particles.
Described herein are method of transferring liquid using a robotic liquid handier from a reagent reservoir having a sloped bottom along a length of the reagent reservoir, the sloped bottom defining a shallow end and a deep end of the reagent reservoir, wherein the shallow end is proximal to a first side-wall of the reagent reservoir, wherein the deep end is proximal to a second side-wall of the reagent reservoir opposite the first side-wall.
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
A server stores a set of laboratory applications to process batches of samples. The server receives, from a first lab administrator, a selection of a subset of the laboratory applications to process the batches of samples and an admin configuration for a laboratory application in the subset. The server receives configuration(s) of batch(es) to be used for running at least a portion of the subset of laboratory applications configured according to the admin configuration. The server receives, from a first scientific device, a request to run a laboratory application form the first subset to process a batch. The server provides the laboratory application(s) that are capable of being executed using the first scientific device. The server receives, from the first scientific device, a selected laboratory application. The server transmits, to the first scientific device, a signal for executing a first part of the selected laboratory application.
A reaction vessel comprises a lower chamber with a first volume, and an upper chamber with a second volume greater than the first volume. A thermocycling system for heating the reaction vessel includes a lower heating zone to heat the lower chamber, an upper heating zone to heat the upper chamber, and a lid heater to heat an opening of the upper chamber. A method comprises loading a sample into a lower chamber of a reaction vessel, thermocycling the lower chamber using a lower heating zone of the thermo cycler, combining an additive into the sample to produce a combination filling the lower chamber and at least partially filling an upper chamber of the reaction vessel, and incubating the upper and lower chambers using the lower heating zone and an upper heating zone. The lower and upper chambers can have different wall thicknesses to facilitate heat transfer.
Systems and methods of identifying reagents loaded into a fluid handling system can comprise programming a protocol for preparing a sample into a controller of the fluid handling system, loading multiple reagent vessels into a carousel of the fluid handling system, imaging individual labels of the multiple reagent vessels with an imaging device to produce label images, comparing information in the label images to identification information located in a database in communication with the controller, and determining if appropriate reagents have been loaded into the carousel to perform the protocol. A fluid handling system can include a deck, a tube holder, an imaging device, a pipettor, a non-transitory computer-readable storage medium and a processor configured to perform the method.
A laboratory workstation for preparing a sample according to a programmed protocol. The laboratory workstation may include a display device configured to display an instruction for loading a first item of labware onto a deck of the laboratory workstation at a position on the deck specified by the programmed protocol; an imaging device configured to monitor the deck of the laboratory workstation by creating one or more images of the deck; and a processor configured to recognize, in the one or more images created by the imaging device, an item of labware loaded onto the deck by an operator. In some embodiments, the processor may be configured to indicate on the display device whether the recognized item of labware loaded by the operator is arranged on the deck in accordance with the programmed protocol.
Water-soluble photoactive polymers, included polymer tandem dyes, as described as well as methods for their preparation and use. The photoactive polymers can be prepared by direct modification of core polymers (e.g., violet excitable polymers) with dyes or other functional groups. Methods of detecting analytes using the polymers are also described.
C09B 69/10 - Polymeric dyes; Reaction products of dyes with monomers or with macromolecular compounds
C09K 11/02 - Use of particular materials as binders, particle coatings or suspension media therefor
C08G 61/10 - Macromolecular compounds containing only carbon atoms in the main chain of the macromolecule, e.g. polyxylylenes only aromatic carbon atoms, e.g. polyphenylenes
A pipetting device is disclosed. The pipetting device can include a piston with a stepped profile. The piston can be inserted into a barrel and can have at least two seal elements. The pipetting device can operate in a low volume dispense mode and a high volume dispense mode.
A magnetic particle is disclosed. The magnetic particle comprises a magnetic material having a maximum field strength in a range of from about 20 emu/g to about 250 emu/g and a remanence in a range of from about 0 emu/g to about 30 emu/g. The magnetic particle further comprises an outer surface containing a ligand. The ligand interacts with an analyte of interest in the sample solution.
H01F 1/00 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
H01F 1/36 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of soft-magnetic materials non-metallic substances, e.g. ferrites in the form of particles
This disclosure is directed to methods and devices (300) associated with Point of Care medical testing and diagnostics. More specifically, methods and devices are described which provide a quick and streamlined way to prepare blood samples for analysis using flow cytometers, microscopes, and other analysis platforms. The benefits include a reduction in the time, resources, and expertise needed for preparing those blood samples without compromising the accuracy and efficacy of diagnosing diseases or identifying specific particulates from those blood samples.
The present invention relates to methods and compositions for lysing red blood cells (RBCs). Current RBC lysis methods have serious drawbacks, and often require a variety of specialized buffers and thus are difficult to be used in work-flow automation for blood- based samples. The invention enables rapid lysis of RBCs in whole blood samples under isotonic, neutral pH conditions. The method according to the invention comprises adding a hypertonic buffer to shrink the cells, using a low concentration of fixative to lightly fix the cells and prevent transporters on the surface of RBCs from opening to compensate for changes in osmotic pressure, and then adding a hypotonic buffer to dilute the sample and restore the sample to an isotonic condition. Upon return to the isotonic condition, the white blood cells (WBCs) swell back up, while the RBCs and platelets are not able to compensate and thus rupture.
G01N 33/96 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Disclosed herein are alkaline phosphatase chemiluminescent substrate formulations. The described formulations generally comprise, in an aqueous solution, a chemiluminescent compound of formula I or a salt thereof, a cationic aromatic compound, a background reducing agent, and an ether-linked nonionic surfactant or a hydrophilic polymer, wherein the ether-linked nonionic surfactant is free of a carboxylate ester group. The described formulations exhibit rapid incubation periods and improved stability for use in immunoassays.
The present invention provides water soluble photoactive macromolecular complexes and methods for detecting an analyte in a sample by using a binding agent conjugated to a water soluble photoactive macromolecule.
The present invention provides methods of determining and quantifying the subcellular localization of an analyte within a sample of cells by using at least two permeabilizing reagents.
An embodiment of a reagent container includes a bottle with a pipe to reduce the effects of reagent sloshing. The bottle has an elongated base and an opposed cover connected by side walls and an end wall. A flat platform surrounded by a raised rim lies in the base opposite an opening in the cover. A ribbed pipe frictionally fits within the bottle opening and may attach to the anchor region leaving vent passages around the pipe. The pipe includes an aperture adjacent to the anchor region and oriented toward the end wall so that sloshed fluid has only a small effect on the level of reagent in the pipe during transfers. A modified blow molding process produces the anchor region by extending a pin a predetermined distance into a mold while the molded material is still plastic.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
24.
METHOD FOR LABELING INTRACELLULAR AND EXTRACELLULAR TARGETS OF LEUKOCYTES
The staining of leukocytes with binding agents such as monoclonal antibodies is an important task, routinely performed in basic research and diagnostic applications. Known procedures are time consuming and quite labor intensive. Further, there are at least two mandatory centrifugation steps, rendering automation of the procedure almost impossible. The present invention provides an improved method for the labeling of intracellular and extracellular targets of leukocytes that is fast and not very labor intensive, does avoid any centrifugation steps, allows the use of binding agent cocktails, and can be easily automated. In particular, by choosing proper reagent compositions and dilution ratios with regard to each step of adding a solution, all time- and labor-consuming centrifugation steps can be omitted. Moreover, a soft fixation step maintains the surface structures of the leukocytes intact.
The present specification discloses regulatory T cells, compositions including regulatory T cells, methods of identifying, isolating, enriching, obtaining, and/or expanding regulatory T cells or subset populations thereof, kits including components useful for performing such methods, and methods of treating an immune-based disorder in an individual by administering regulatory T cells or compositions comprising such regulatory T cells to an individual in need thereof.
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
G01N 33/48 - Biological material, e.g. blood, urine; Haemocytometers
The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.
Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of contain-ing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemi-luminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.
C12Q 1/28 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
G01N 33/542 - Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
28.
SYSTEM AND METHOD FOR AUTOVERIFYING LABORATORY TEST RESULTS
A method of autoverifying clinical test results comprises displaying an autoverification process as a flowchart on a graphical user interface. The autoverification process is defined by a plurality of nodes and a plurality of edges connecting the nodes. The autoverification process is configured to evaluate a result and determine if the test result meets a predetermined criteria. The method further comprises receiving the test result and automatically performing the autoverification process on the test result. A system for creating and implementing the autoverification processes comprises a graphical user interface configured to display the autoverification process as a flowchart. The system includes an input configured to receive the clinical test result from a laboratory analyzer. The system also includes a processor configured to analyze the clinical test result according to the defined autoverification process.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 37/00 - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES - Details not covered by any other group of this subclass
patents
