The present disclosure provides, among other things, systems, methods, and kits include internal amplification controls. Provided internal amplification controls are or include non-target sequences that are amplified during nucleic acid amplification. Provided internal amplification controls are linearly amplified. Provided internal amplification controls are useful for systems, methods and kits for nucleic acid amplification.
A fluid handling apparatus having a two-sided channel plate with channel ports extending from one side to the other. Fluid channels on both sides of the channel plate connected to the channel ports direct fluid through the channel plate in a desired fluid path, and the first face and the second face of the channel plate are sealed to fluidly encase the fluid channels.
The present disclosure provides a substantially sealed system and methods of use thereof for nucleic acid amplification. Said system includes, for example a base, a nucleic acid amplification reaction vessel, a collection cap, a storage cap and/or a housing. The system reduces contamination before, during and after the amplification reaction. Therefore said system reduces amplification of spurious nucleic acids or amplification products. The present disclosure also provides methods of amplifying nucleic acids using said system.
The present invention provides, in addition to other things, methods, systems, and apparatuses that involve the use of an optical fiber with grating and particulate coating that enables simultaneous heating; optical detection; and optionally temperature measurement. Methods, systems, and apparatuses of the present invention may be used in many applications including isothermal and/or thermal cycling reactions. In certain embodiments, the present invention provides methods, systems, and apparatuses for use in detecting, quantifying and/or identifying one or more known or unknown analytes in a sample.
The present disclosure provides methods, systems, and apparatuses for collecting and/or amplifying nucleic acids. In general, provided methods, systems, and apparatuses involve contacting a sample including a nucleic acid with a nucleic acid amplification reagent without purification of nucleic acids from the sample.
The speed of a nucleic acid amplification reaction, such as the Polymerase Chain Reaction (PCR), can be increased by setting the temperature of heat sources above and below the desired denaturation, annealing, and extension temperatures. The reaction mixture is only contacted with the heat sources long enough for the desired temperatures to be reached. A non-uniform temperature gradient is created from top to bottom, in the liquid, inside the reaction vessels.
A system is provided for carrying out rapid nucleic acid amplification or other biological reactions requiring thermal cycling. The system of this invention incorporates at least two heating blocks, each having a groove for receiving a reaction vessel such that only a portion of the outer surface of the walls of the vessel are in direct contact with the heating block. The remaining portion of the outer surface of the walls of the reaction vessel remains exposed to ambient conditions. The reaction vessel comes into contact with only one heating block at a time either by movement of the vessel between the heating blocks or by movement of the heating blocks in relation to the vessel. The system of this invention provides rapid temperature cycling without the need for extended ramping times generally associated with single block designs, which include a single temperature block that is forced to heat and cool. Heating and cooling of the reaction using the system of the present invention is accomplished by the reaction vessel and heating blocks coming into thermal contact and reaching thermal equilibrium. The entire vessel need not be surrounded by the heating block, with at least one side of the vessel partially open to ambient conditions. As a result of the configuration of this system it is readily combined with detection systems, for example, fluorescence detection systems.