Provided is a directionally driven hemostatic microsphere with a puncture function, which comprises hollow pollen and a power system assembled on the surface of the hollow pollen, wherein the surface of the hemostatic microsphere is needled or spiked. Further provided is a preparation method for the directionally driven hemostatic microsphere with the puncture function, which comprises the following three steps: preparation of a hollow pollen microsphere, development of a power system on the pollen microsphere, and loading of a hemostatic drug thrombin. According to the present invention, by puncturing red blood cells, the release of blood coagulation substances and the adsorption of platelets, red blood cells, and blood coagulation factors are promoted, and the formation of a fibrin network is promoted, so that the rapid hemostasis of a wound is achieved.
A61L 24/02 - Surgical adhesives or cements; Adhesives for colostomy devices containing inorganic materials
A61L 24/00 - Surgical adhesives or cements; Adhesives for colostomy devices
A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
A61K 47/52 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an inorganic compound, e.g. an inorganic ion that is complexed with the active ingredient
A61K 41/00 - Medicinal preparations obtained by treating materials with wave energy or particle radiation
A61K 38/48 - Hydrolases (3) acting on peptide bonds (3.4)
Disclosed in the present invention are a breeding method of a blue marked two-line hybrid wheat system and the use. According to the present invention, an exogenous translocation chromosome [T4AgL (a Ba-containing fragment)-4BL (a fragment close to a kinetochore).4thS and T4AgL (a Ba-containing fragment)-4BL (a fragment close to a kinetochore).4thS (a Rf-containing fragment)] that has a capability of completely recovering a recessive genic male sterile gene ms1 and contains a blue grain gene Ba exhibiting dose effect and xenia and an exogenous restoring gene Rf, and an exogenous translocation telomere [T4AgL (a Ba-containing fragment)-4thS (a Rf-containing fragment)., T4thS (a Rf-containing fragment)-4AgL (a Ba-containing fragment) and T4thS (a Rf-containing fragment)-4AgL (a Ba-containing fragment)-4BL (a fragment close to a kinetochore).] are created. As a result, an improvement in the blue marked two-line hybrid wheat system is achieved.
Provided are a bombyx mori silk gland recombinant expression vector expressing a human epidermal growth factor, a preparation therefor, and an application thereof. The recombinant expression vector is formed by fusing an N-terminal domain and a C-terminal domain of a truncated silk fibroin heavy chain, an optimized human epidermal growth factor, and a bombyx mori nuclear polyhedrosis virus enhancer. A redundant part of an extra domain of an endogenous silk protein contained in a fusion protein is removed to the maximum extent, and on the basis of not affecting a genetic hybridization process of integrating a fusion exogenous target protein into a silk-based material, redundant post-translational modification of the fusion protein is eliminated as much as possible, thereby creating, by means of genetic hybridization, a functional silk material expressing a highly biologically active human epidermal growth factor, and providing a feasible solution for development of a novel highly biologically active silk-based material by means of an advanced biosynthesis platform represented by bombyx mori.
SUZHOU UNIVERSITY OF SCIENCE AND TECHNOLOGY (China)
Inventor
Li, Changming
Zeng, Qingxin
Abstract
A low-altitude air route and road integrated management platform and method. The low-altitude air route and road integrated management platform comprises a cloud database, a center management platform and a terminal user, wherein the center management platform generates path planning information and sends the path planning information to the terminal user; the terminal user controls, according to the path planning information, a corresponding terminal device to move along a planned path; the terminal user acquires motion trajectory state information, uploads the motion trajectory state information to the cloud database and updates the cloud database in real time; and the center management platform generates path planning correction information according to the updated cloud database and downloads the path planning correction information to the terminal user to control the real-time motion state of the terminal device.
Provided is an application of the tomato hydroxyproline-rich systemin precursor protein gene SlHypSys in improving the resistance of plants to Verticillium wilt. By means of cloning the tomato hydroxyproline-rich phylogenetic precursor protein gene, SlHypSys, and using molecular biology methods to construct a plant expression vector for constitutive expression of SlHypSys, then using genetic engineering methods to introduce the SlHypSys gene into a plant, transgenic Arabidopsis thaliana, tobacco, and cotton strains having normal transcriptional expression are obtained; the disease indices of all of the obtained transgenic plants are significantly lower than those of the wild-type control, resistance to Verticillium wilt is significantly higher, clearly indicating that the SlHypSys gene can be used for improving the resistance of plants to Verticillium wilt.
A dual-support electric drive wheel, comprising a box body combination, a drive motor (2), a speed changing assembly and a hub (15). The drive motor (2) and the speed changing assembly are located within the box body combination, a first support part (1) is formed on a left side of the box body combination, and a fixing shaft (10) extends out from within a box body on the right side to form a second support part (18); and the first support part (1) and the second support part (18) are used for mounting and supporting the drive wheel. The dual-support electric drive wheel being installed on a rear support structure of a vehicle is stable, improves driving safety, and improves load capacity. Meanwhile, the structure has sufficient installation space, can be provided with a speed reducing mechanism, is suitable for being driven by using a high-speed motor, can greatly improve the torque output range of an electric vehicle, finally achieve a good match between driving resistance and the output torque, and improve working efficiency. At the same time, the dual-support structure used has good stress conditions, good overall stability, can reduce the volume and weight of each support, is not affected by deflection torque, reduces fabrication costs, and reduces energy consumption while ensuring operation stability.
B60K 7/00 - Disposition of motor in, or adjacent to, traction wheel
B60K 17/08 - Arrangement or mounting of transmissions in vehicles characterised by arrangement, location, or kind of gearing of change-speed gearing of mechanical type
H02K 7/116 - Structural association with clutches, brakes, gears, pulleys or mechanical starters with gears
Provided is the use of cyclovirobuxine in the preparation of a cell autophagy inducer; cyclovirobuxine can be used for treating autophagy-related diseases and for constructing a cell autophagy model drug.
A61K 31/57 - Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone
Disclosed are fucose-rich extracellular polysaccharide, a preparation method therefor and an application thereof. The polysaccharide consists of L-fucose, D-glucose, D-galactose, D-glucuronide and pyruvate units, and the extracellular polysaccharide has reducing properties and can be used as a reducing agent and a stabilizer. Nano-silver having a uniform size is prepared by means of a green method, an EPS/nano-silver antibacterial film is further prepared, and the prepared film has an antibacterial effect and is applied in the aspect of biodegradable antibacterial films. Fuco-oligosaccharide hydrolyzed by the extracellular polysaccharide has the functions of promoting carbohydrate metabolism as well as the biosynthesis and metabolism of sugar, reducing membrane transport, enhancing digestive system function or reducing infection by disease, promoting short-chain fatty acid production in the intestines and improving the level of intestinal microflora, can be used to prepare intestinal prebiotics for human or livestock animals, and has wide application prospects.
A central drive type electric drive system assembly, comprising a rotating output component (1) and a transmission shaft (2) passing through the rotating output component (1). A power system and a speed change system are installed on the transmission shaft (2). The power system and the speed change system are respectively located on two sides of the rotating output component (1). The power system transmits power to the speed change system by means of the transmission shaft (2), and the speed change system drives the rotating output component (1) to rotate to output the power. The newly designed central drive type structure has the advantages of both a hub motor and a side-mounted structure, makes up for the deficiencies of the hub motor and the side-mounted structure, is especially suitable for a two-wheeled vehicle, not only can well ensure the rotating output component (1), but also has high mechanical efficiency, less heat generation, better heat dissipation capacity and lighter weight.
A self-adaptive multi-disc actuated in sequence large-torque friction clutch device with a one-way transmission function, the device comprising a power input mechanism used for inputting power and a main shaft (1) used for outputting power, wherein an inner plate spiral raceway sleeve (5), a friction clutch (2) and an elastic element set (3) are arranged on the main shaft (1) in a sheathed manner, the friction clutch (2) comprises a friction plate supporting member arranged on the inner plate spiral raceway sleeve (5), a plurality of outer friction plates (2c) and a plurality of inner friction plates (2d), the outer friction plates (2c) and the inner friction plates (2d) are alternately arranged between the friction plate supporting member and the inner plate spiral raceway sleeve (5), the outer friction plates (2c) can axially slide along the friction plate supporting member, and the inner friction plates (2d) can axially slide along the inner plate spiral raceway sleeve (5). By using the solution, one-way transmission of power can be achieved, and meanwhile, the multiple outer friction plates (2c) and the multiple inner friction plates (2d) that are alternately arranged are utilized, the bearing torque is dispersed on the outer friction plates (2c) and the inner friction plates (2d), such that sliding friction loss is greatly reduced, the defect that a traditional disc type friction clutch is poor in abrasion resistance is overcome, and the overall device is high in stability and reliability and has a long service life.
An intelligent self-adaptive automatic transmission, comprising a power input mechanism, a forward gear transmission system, a reverse gear transmission system, a main shaft (1) used for outputting power, and a transmission sensing mechanism used for measuring resistance torque borne by the main shaft (1); wherein the power input mechanism simultaneously drives the reverse gear transmission system and the transmission sensing mechanism, and the transmission sensing mechanism drives the forward gear transmission system. The transmission sensing mechanism comprises a forward gear primary driven gear shaft (19) driven by the power input mechanism, a forward gear secondary driving gear (20) used for transferring power to the forward gear transmission system, a transmission sensing cam sleeve (21) capable of being axially slidably sleeved on the forward gear primary driven gear shaft (19), and a displacement measurement device (25) used for measuring the displacement of the transmission sensing cam sleeve (21), wherein the transmission sensing cam sleeve (21) can synchronously rotate under the driving of the forward gear primary driven gear shaft (19); the forward gear secondary driving gear (20) is rotatably sleeved on the forward gear primary driven gear shaft (19) and transmittingly works in conjunction to a corresponding end surface of the transmission sensing cam sleeve (21) by means of an end surface cam pair, so that the transmission sensing cam sleeve (21) can be driven to be far away from the forward gear secondary driving gear (20); an elastic reset element (24) is provided between the transmission sensing cam sleeve (21) and the forward gear primary driven gear shaft (19), so that the transmission sensing cam sleeve (21) can be driven to be close to the forward gear secondary driving gear (20). The device can adaptively adjust the rotating speed and the torque of a motor, so that the motor is in a working state with high rotating speed and high efficiency, the energy consumption is low, and the stability, reliability and service life of the motor are improved; moreover, a special reverse gear transmission system is additionally provided, so that the energy of the motor is recovered, a larger speed ratio can be provided during vehicle reversing, the output torque is larger, and the device is facilitated overcoming more extreme conditions and improving the vehicle reversing safety.
F16H 3/093 - Toothed gearings for conveying rotary motion with variable gear ratio or for reversing rotary motion without gears having orbital motion exclusively or essentially with continuously- meshing gears, that can be disengaged from their shafts characterised by the disposition of the gears with two or more countershafts
F16D 41/067 - Freewheels or freewheel clutches with intermediate wedging coupling members between an inner and an outer surface the intermediate members wedging by rolling and having a circular cross-section, e.g. balls all members having the same size and only one of the two surfaces being cylindrical and the members being distributed by a separate cage encircling the axis of rotation
12.
COMPACT AND ULTRA-LARGE LOAD ADAPTIVE AUTOMATIC TRANSMISSION SYSTEM
A compact and ultra-large load adaptive automatic transmission system, comprising a motor (17), a common deceleration mechanism, a forward gear transmission system, and a transmission bridge (1) for outputting the power. A transmission shaft (1c) and a second transmission shaft (1d) can directly drive left and right front wheels of a vehicle to rotate to realize the power output of the front-engine, front-wheel-drive layout. The whole transmission bridge (1) has high transmission efficiency, and is simple, stable, and reliable in structure. Moreover, the motor (17) can directly transfer the power to the front gear transmission system and a reverse gear transmission system of a transmission by means of the common deceleration mechanism, which reduces the number of parts, simplifies the structure of the transmission system, reduces the volume of the transmission system, makes the transmission system more compact, and reduces the assembling difficulty. At the same time, by making improvements on a multi-row overrunning clutch (6) and a multi-plate friction clutch (2), the adaptive automatic transmission system can bear ultra-large load, which improves the reliability and reduces manufacturing costs.
F16H 3/10 - Toothed gearings for conveying rotary motion with variable gear ratio or for reversing rotary motion without gears having orbital motion exclusively or essentially with continuously- meshing gears, that can be disengaged from their shafts with one or more one-way clutches as an essential feature
F16H 37/08 - Combinations of mechanical gearings, not provided for in groups comprising essentially only toothed or friction gearings with arrangements for dividing torque between two or more intermediate shafts with differential gearing
F16D 41/064 - Freewheels or freewheel clutches with intermediate wedging coupling members between an inner and an outer surface the intermediate members wedging by rolling and having a circular cross-section, e.g. balls
A multi-row floating combined type heavy load overrunning clutch comprising an inner center wheel cam sleeve (7), an outer ring (6a) and at least two inner center wheels (6c) arranged between the inner center wheel cam sleeve (7) and the outer ring (6a) side by side, wherein rolling bodies (6d, 6e) are arranged between the outer ring (6a) and the inner center wheels (6c) respectively, outer teeth (6c1) on the peripheries of the inner center wheels (6c) directly are opposite in a one-to-one correspondence manner, and the rolling bodies (6d, 6e) around the adjacent inner center wheels (6c) are arranged directly opposite in a one-to-one correspondence manner. The structure is novel, the design is ingenious, the number of inner center wheels (6c) and the corresponding rolling bodies (6d, 6e) can be infinitely increased according to actual needs, due to the fact that the inner center wheels (6c) and the rolling bodies (6d, 6e) are short in length, stress is uniform, reliability is high in the usage process, the situation that the rolling bodies (6d, 6e) are broken is avoided, while production machining precision requirements are low, manufacturing is easy, the cost is low, and assembly is simple, such that the heavy load overrunning clutch which is extremely high in reliability and can bear a super-large load can be manufactured at relatively low production cost.
F16D 41/064 - Freewheels or freewheel clutches with intermediate wedging coupling members between an inner and an outer surface the intermediate members wedging by rolling and having a circular cross-section, e.g. balls
F16D 47/04 - Systems of clutches, or clutches and couplings, comprising devices of types grouped under at least two of the following sets of groups: ; ; ; ; of which at least one is a freewheel
14.
ULTRA-LARGE TORQUE, DOUBLE-HELIX, DOUBLE-OVERRUNNING, AND INTEGRATED INTELLIGENT ADAPTIVE ELECTRIC-DRIVE FRONT DRIVING SYSTEM
An ultra-large torque, double-helix, double-overrunning, and integrated intelligent adaptive electric-drive front driving system, comprising a motor, a main shaft (1), a high-speed gear transmission mechanism, a low-speed gear transmission mechanism, and a front driving force output mechanism. The motor comprises a stator (15), a rotor (16), and a motor shaft assembly that is driven by the rotor (16); the front driving force output mechanism comprises a power output gear sleeve (34) and a differential (35); the power output gear sleeve (34) is rotatably sleeved on the main shaft (1). Compared with a traditional single row of overrunning clutches, multiple rows of overrunning clutches (6) can exponentially improve a load-carrying ability, and break though the carrying limit of a traditional overrunning clutch; the power output of front-engine front-wheel drive arrangement can be achieved by means of the design of the differential (35) and related components, and an entire transmission bridge is high in transmission efficiency; and therefore, in the case that a driving force is not cut off, gear-shifting transmission is adaptively and automatically performed along with the change of traveling resistance, thereby making the motor be always located on an efficient platform as much as possible, and greatly increasing an interval in which the motor efficiently operates.
B60K 1/00 - Arrangement or mounting of electrical propulsion units
B60K 17/02 - Arrangement or mounting of transmissions in vehicles characterised by arrangement, location, or kind of clutch
B60K 17/08 - Arrangement or mounting of transmissions in vehicles characterised by arrangement, location, or kind of gearing of change-speed gearing of mechanical type
A fully-mechanical self-adaptive automatic transmission, comprising a power input mechanism, a high-speed gear transmission mechanism, a low-speed gear transmission mechanism, and a main shaft (1) for outputting power. The high-speed gear transmission mechanism comprises a friction clutch (2) and an elastic element group (3) for applying a preload force to the friction clutch (2); the power input mechanism transfers power to the friction clutch (2) by means of a first overrunning clutch (4), the friction clutch (2) is sleeved on the main shaft (1) by means of an inner plate spiral raceway sleeve (5), and a spiral transmission pair is formed between the inner plate spiral raceway sleeve (5) and the main shaft (1) to enable the inner plate spiral raceway sleeve (5) to axially slide along the main shaft (1); the low-speed gear transmission mechanism comprises a second overrunning clutch (6) and an auxiliary shaft transmission assembly for speed reduction transmission between the power input mechanism and the second overrunning clutch (6), the second overrunning clutch (6) is sleeved on the main shaft (1) by means of an inner core wheel cam sleeve (7), and the inner core wheel cam sleeve (7) can transfer power to the main shaft (1) by means of the inner plate spiral raceway sleeve (5). The apparatus can automatically perform gear shifting and speed changing adaptively with traveling resistance changes under the condition that driving force is not cut off, the gear shifting is smooth, the efficient running interval of a motor is greatly increased, the apparatus can be used in mountainous areas, hills and heavy load conditions, so that the load change of the motor or an engine is gentle, and the whole vehicle runs stably and is high in safety.
F16D 41/064 - Freewheels or freewheel clutches with intermediate wedging coupling members between an inner and an outer surface the intermediate members wedging by rolling and having a circular cross-section, e.g. balls
Provided is a hyoscyamine aldehyde reductase with the amino acid sequence shown in SEQ ID NO. 4. Also provided are a gene, a recombinant expression vector, a transgenic cell line, and a recombinant bacteria of the hyoscyamine aldehyde reductase, the application thereof in catalysing the reduction of hyoscyamine aldehyde to produce hyoscyamine, and the application thereof in rebuilding the hyoscyamine synthesis pathway or increasing the hyoscyamine content.
A dual-overrunning clutch shaft sleeve output adaptive automatic transmission main shaft assembly, a gearbox, and a drive system, comprising a main shaft (1), and a transmission system and a power output sleeve on the main shaft (1), the transmission system comprising a low-speed gear power input member, a reverse gear power input member, and an adaptive transmission assembly; the reverse gear power input member has a transmission ratio I and the low-speed gear power input member has a transmission ratio II, transmission ratio I being greater than or equal to transmission ratio II; using the rational engagement of two overrunning clutches, the transmission ratios of the reverse gear structure and the low-speed gear mechanism are set rationally so that the entire structure is simple and compact and interference does not occur, ensuring overall performance and transmission stability; using the output power of the shaft sleeve, the output part can be selected according to requirements, being applicable to the field of electric vehicles and also applicable to the field of other variable torque mechanical transmissions; in addition, the shaft sleeve output can also ensure a large output torque.
An adaptive automatic transmission main shaft assembly employing a double overrunning clutch-based mechanical shaft end-output, a transmission, and a drive system. The assembly comprises a transmission system, a shaft sleeve, and a third shaft. The transmission system comprises a low gear transmission mechanism, a reverse gear transmission mechanism, and an adaptive transmission assembly. The reverse gear mechanism has a transmission ratio I for transferring a reverse gear drive from a secondary shaft (12) to a main shaft (1). The low gear transmission mechanism has a transmission ratio II for transferring a low gear drive from the secondary shaft (12) to the main shaft (1). The transmission ratio I is greater than or equal to the transmission ratio II, and is used to output a power via a power outputting shaft. The invention utilizes the logical cooperation of two overrunning clutches, and configures appropriate transmission ratios, such that the overall structure is simple and compact, and reverse gear transmission shares a transmission path with low gear transmission and high-speed gear transmission without causing interference, thereby ensuring overall performance. The invention further utilizes a transmission shaft sleeve and provides an output via the third shaft, thereby being applicable to both electric vehicles and other variable-torque mechanical transmissions. In addition, a shaft sleeve-based output further ensures a large output torque.
F16H 61/28 - Generation or transmission of movements for final actuating mechanisms with at least one movement of the final actuating mechanism being caused by a non-mechanical force, e.g. power-assisted
B60K 17/08 - Arrangement or mounting of transmissions in vehicles characterised by arrangement, location, or kind of gearing of change-speed gearing of mechanical type
19.
MECHANICAL DUAL-OVERRUNNING CLUTCH SHAFT OUTPUT ADAPTIVE AUTOMATIC GEARBOX SHAFT ASSEMBLY, GEARBOX AND DRIVE SYSTEM
A mechanical dual-overrunning clutch shaft output adaptive automatic gearbox shaft assembly, a gearbox and a drive system, comprising a shaft (1) outputting force and a shifting system on the shaft (1), the shifting system comprising a low-speed gear transmission mechanism, a reverse gear transmission mechanism and an adaptive shifting component; the reverse gear mechanism having a transmission ratio I transmitting reverse gear force from a countershaft (12) to the shaft (1), and the low-speed transmission mechanism having a transmission ratio II transmitting low-speed gear force from the countershaft (12) to the shaft (1), the transmission ratio I being greater than or equal to the transmission ratio II. Using reasonable matching of two overrunning clutches and reasonably configuring transmission ratios of a reverse gear mechanism and a low-speed gear mechanism makes an overall structure simple and compact; the reverse gear transmission sharing a transmission path with the low-speed gear and a fast gear without generating interference ensures overall functionality of the mechanical adaptive automatic gearbox; adaptability is relatively strong, and matching with an adaptive automatic gearbox mechanism is smooth and natural; the present invention is not only for for use in the electric vehicle field, but can also be used in other torque conversion mechanical transmission fields.
Disclosed is the use of tuberculosis markers, the BATF2, UBE2L6, VAMP5 and/or SERPING1 genes, and mRNA, cDNA, proteins, or detection reagent thereof for preparing a diagnostic reagent or kit, wherein the diagnostic reagent or kit is used to diagnose tuberculosis and/or evaluate a tuberculosis treatment effect of tuberculosis. The tuberculosis markers are used for tuberculosis diagnosis and efficacy evaluation, and have high sensitivity and strong specificity.
Provided are a gene GhLFHE2 and an encoded protein and application thereof. The cDNA nucleotide sequence of the gene is shown as SEQ ID No. 1. By constructing a plant expression vector by using the gene GhLFHE2, transforming a host, and further transforming cotton, the cotton fiber of the obtained transgenic cotton is extended.
Disclosed are a sweet potato sitosterol glycoside saturated fatty acid ester, an extract and a preparation method therefor. The sweet potato sitosterol glycoside saturated fatty acid ester extract has a certain inhibitory effect on MCF-7, and the inhibitory effect is weak in low concentration. The sweet potato sitosterol glycoside saturated fatty acid ester has good inhibitory effect on MCF-7 breast cancer cells, wherein sweet potato sitosterol glycoside palmitate has the strongest inhibitory effect, and increase and decrease in carbon chains of the saturated fatty acid decreases the antitumor activity to a certain extent.
A61K 31/704 - Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin, digitoxin
A61K 36/39 - Convolvulaceae (Morning-glory family), e.g. bindweed
The present invention relates to the field of medicinal chemistry, and specifically to a sweet potato sitosterol glycoside unsaturated fatty acid, an extract, a preparation method therefor, and an application thereof. Sweet potato sitosterol glycoside linolenic acid ester has a significant effect on inhibiting the growth of tumor cells. In the case of an administration concentration of 10 mg/L, the inhibition rate of a sweet potato sitosterol glycoside linolenic acid ester monomer of the present invention on MCF-7 cells is 92%, the inhibition rate of a sweet potato sitosterol glycoside linoleic acid ester monomer on MCF-7 cells is 65%, and the inhibition rate of a sweet potato sitosterol glycoside monomer on MCF-7 cells is 55%; in the case of an administration concentration of 1 mg/L, the inhibition rate of a contrastive sweet potato sitosterol glycoside linolenic acid ester monomer on MCF-7 cells is 45%, the inhibition rate of a sweet potato sitosterol glycoside linoleic acid ester monomer on MCF-7 cells is 15%, and the inhibition rate of a contrastive drug, i.e., a sweet potato sitosterol glycoside monomer on MCF-7 cells is 10%.
C07J 17/00 - Normal steroids containing carbon, hydrogen, halogen, or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta[a]hydrophenanthrene skeleton
A23L 33/11 - Plant sterols or derivatives thereof, e.g. phytosterols
A61K 36/39 - Convolvulaceae (Morning-glory family), e.g. bindweed
Provided is a use of a Beauveria bassiana BbP4-ATPase gene to improve plant resistance to Verticillium wilt, and a method for improving plant resistance to Verticillium wilt; a Beauveria bassiana BbP4-ATPase gene is expressed in a target plant, thereby improving the resistance of said plant to Verticillium wilt.
Provided is a use for an encoding VdP4-ATPase gene of Verticillium wilt P-type ATPase to improve plant resistance to Verticillium wilt. Also provided is a method for improving plant resistance to Verticillium wilt, whereby an encoding VdP4-ATPase gene of Verticillium wilt P-type ATPase is expressed in a target plant, thereby improving the resistance of said plant to Verticillium wilt.
Disclosed is a method for preparing an oxygen reduction electro-catalyst based on iron-tungsten double-metal oxide enhanced nitrogen-doped graphene. The method comprises the following steps: (1) oxidizing a graphite powder to prepare a graphite oxide; (2) ultrasonically processing the graphite oxide prepared in the step (1) to prepare a graphene oxide; (3) diluting the graphene oxide prepared in the step (2) with water, and mixing with a nitrogen source and performing a hydrothermal reaction to obtain a nitrogen-doped graphene; (4) dispersing the nitrogen-doped graphene prepared in the step (3) into the water, adding an iron source and a tungsten source, heating and performing a hydrolysis reaction; and (5) washing and drying the reaction products in the step (4), and then performing a thermal process under the conditions of a protection gas. The catalyst prepared by the method of the present invention has excellent oxygen reduction catalytic performance and long-term stability, and significant methanol catalysis resistance, has a low price, stable performance, a simple preparation method, and can replace the precious metal platinum to be used as the oxygen reduction electro-catalyst of commercialized fuel batteries and the like.
Provided in the present invention is a method for controlling the security of transgenic hybrid crops, which comprises the construction of a recombinase system, transcriptional activation system, and auto-deletion-binary-system of transgenic plants of an exogenous gene expression control system. The transcriptional activation system is controlled by using the specific promoter of a floral primordial cell of a plant as the promoter of the auto-deletion-binary-system of transgenic plants, and the start of the recombinase system is controlled by the transcriptional activation system which makes the exogenous gene introduced into the plant exist in a stable state in the hybrids of the F1 plants and the non-deletion tissues, such as roots, stems, leaves, etc. but the exogenous gene in the pollens and seeds of the F1 plants is deleted in order to achieve security control of the transgenic hybrid crops.
A system and a process for determining and analysing surface property parameters of a substance based on a kinetic method. The system comprises a sample treatment system including a reactor (3), a collector for the liquid to be tested (5) and a container for the liquid to be tested (6), and a detection system including detecting electrodes (13), an operator for concentration and activity, a processor for kinetic data, an operating module for surface properties and a results outputting module. The method treats the substance to be tested with an electrolyte solution, measures the activity of the liquid to be tested after the reaction at a pre-set interval, and processes the data with the processor for kinetic data and the operating module for surface properties, so as to obtain the surface property parameters of the substance to be tested. The present invention employs a kinetic method of ion exchange to overcome the difficulties in the test procedure of having a long reaction equilibrium time, and the difficulty in determining the equilibrium and calibrating the value of m accurately. The five parameters representing the surface properties of a substance can be calculated from only two parameters, i.e. the intercept and gradient of the linear regression equation obtained from the kinetic data, and the total amount of charge on the surface can also be determined directly.
Provided is a method for Bombyx mori silk fibroin heavy chain gene mutation, specifically comprising causing the mRNA of a coded zinc finger nuclease sequence to act upon a target point such as positions 1325-1362 of Bombyx mori silk fibroin heavy chain gene as expressed in SEQ ID NO:1, so as to obtain a series of Bombyx mori silk fibroin heavy chain mutant genes. Also provided is a mutation sequence prepared according to said method. Also provided are uses of said mutation sequence in the preparation of sericin and foreign proteins. Also provided is a Bombyx mori mutant comprising the described mutation sequence as a novel Bombyx mori silk gland bioreactor, the rear silk gland of said mutant being severely degraded and the cocoon layer whereof including only sericin synthesized and excreted by the middle silk gland, whereby the expression volume and purity of foreign transgenes in the rear silk gland of Bombyx mori can be greatly increased and large-scale production of sericin can be achieved.
Constructed is a new Gene-Auto-Excision Binary System (GAEBS) for controlling the biosafety risk of a plant exogenous gene in sexual reproduction. Mainly using the transcription activating system as a molecular switch to control a recombinase excision system, on one hand, the recombinase gene located between the recombinase recognition sites is maintained silent in the sexual generation of a plant and heredity stability of the exogenous gene introduced by "GM-gene-deletor" technology in the sexual reproduction of the parent plant is realized; and on the other hand, by performing hybridization of parent plants carrying an effector element and an activator element respectively, the effector element and the activator element can be linked to present transcription activating factor activity. At the same time, using a plant tissue specific promoter to promote the expression of the recombinase, tissue specific excision of the exogenous gene is realized and a safe transgenic plant is obtained.
Nucleotide sequences of promoter of cotton brassinolide synthetase GhDWF4 gene or its fragment are provided, wherein the promoter is 2600bp in length. The promoter or its fragment is useful for regulating special tissue expressions of target genes in plants to regulate the growth and development of tissue, and to obtain fine agronomic character.
A universal method for jointly measuring surface property parameters of a material comprises the following steps: performing saturation processing on a surface of a material to be measured; mixing the material to be measured which has been processed by saturation with an indicating electrolyte solution; after the mixture solution achieves an balance by ion exchange, measuring the balanced concentration values of positive ions of the indicating electrolyte and H+ in the mixture solution; according to the balanced concentration values of positive ions of the indicating electrolyte, calculating surface potential of the material; and according to the surface potential of the material, calculating specific surface area and surface charge density of the material; according to surface charge density of the material, calculating surface electric field intensity; according to surface charge density and specific surface area of the material, calculating total surface charge of the material. The method could accurately measure different kinds of surface potentials under difference conditions. A system for measuring surface property parameters of a material based on the method is also disclosed.
A method of expressing auxin synthetase gene specifically in cotton seed coat and fiber, which comprising constructing plant expression vector capable of expressing auxin synthetase gene specifically by fusing tissue-specific promoter with auxin synthetase gene, and then integrating the vector into cotton genome. The method can increase the yield of cotton fiber, improve the quality of cotton fiber, thereby providing fiber with good quality for cotton and textile industry.
A cam self-adapting automatic speed-varying hub includes a left hub rack (7), a right hub rack (18), a case (4), a power input device, a transmission shaft (1), a wheel (14) and a braking device (20), and further includes a low speed gear transmission shaft (12), a low speed gear transmission mechanism provided on the low speed gear transmission shaft (12) and a taper plate clutch cam self-adapting speed-varying assembly provided on the transmission shaft (1), and the low speed gear transmission shaft (12) provided inside the case (4) is matched rotatablely with the case (4) and parallel with the transmission shaft (1). Such a cam self-adapting automatic speed-varying hub can remain motor/engine output power and vehicle running state always in the best matching state, thus achieving balance control on the vehicle driving torque and the comprehensive running resistance.
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