Abstract

Computer-implemented methods of providing a disease diagnosis or prognosis for a patient are described. These comprise generating individual networks, each individual network comprising a plurality of nodes and edges between pairs of the nodes, wherein each node is indicative of a biological factor in biological data for an individual, and each edge is indicative of a relationship between a pair of biological factors corresponding to the nodes that the edge connects for the respective individual; determining the value of one or more similarity metrics between one or more individual networks generated for the patient and one or more individual networks generated for other individuals in the plurality of individuals; and predicting a diagnosis or prognosis for the patient using a machine learning model that takes as input the values of one or more similarity metrics.

IPC Classes  ?

• G16B 40/20 - Supervised data analysis
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems

Abstract

Methods for the rapid detection of the presence or absence of Monkeypox Virus (MPXV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the MPXV F3L gene and the MPXV B21R gene, along with kits are provided that are designed for the detection of MPXV.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

The present invention provides a method comprising the steps of a) providing a nucleic acid comprising 5fC, 5hmC, or 5caC, b) providing one reactant comprising two reactive groups wherein the first reactive group is capable of reacting with the formyl hydroxymethyl or carboxyl group, and the second reactive group is a nucleophilic group, c) reacting said first reactive group with the formyl, hydroxymethyl or carboxyl group thereby resulting in a modified 5fC, 5hmC, or 5caC, d) reacting said second reactive group with the C6 position of said modified 5fC, 5hmC, or 5caC, thereby obtaining a bicyclic or tricyclic molecule comprising a 5,6-di-hydro Cytosine entity, and e) deaminating said 5,6-di-hydro Cytosine entity to a 5,6 di-hydro-Uracil entity.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

A biomedical knowledge graph system, the system including a computer database of records, the records comprising nodes of biomedical entities and connections between the entities representing biomedical relationships. One or more processors are programmed and configured to extract data from a plurality of data sources, determine biomedical entities and relationships between the entities based on analyzing the data, wherein analyzing the data comprises searching for predetermined identifiers or patterns in the data. Based on the determined biomedical entities, assigning each biomedical entity to a cluster of biomedical entity types. The one or more processors are configured to identify a context for each of the identified biomedical entities based on the assigned cluster and based on elements of the expression of the biomedical data within which the entity is expressed. Based on the identified context and type of the biomedical entity, incorporating records of nodes and connections between nodes into the knowledge graph, the nodes representing biomedical entities and the connections representing biomedical relationships between the entities structured according to the predefined schema.

IPC Classes  ?

• G16B 50/10 - Ontologies; Annotations
• G16B 50/20 - Heterogeneous data integration
• G16B 45/00 - ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks
• G06F 16/332 - Query formulation

Abstract

The present disclosure relates, in general, to novel and easily accessible fluorescent compounds with large Stokes shift (LSS) and thermostable fluorescence for expanding the multiplexing capabilities of fluorescence-based nucleic acid detection technologies. Moreover, conjugates, probes and FRET pairs comprising these fluorescent compounds as well as methods for amplification and detection of a target nucleic acid utilizing these fluorescent compounds and methods of labeling are also provided.

IPC Classes  ?

• C09B 11/24 - Phthaleins containing amino groups
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

Abstract

The present disclosure relates, in general, to the methods for the rapid detection of the presence or absence of Lymphogranuloma Venereum (LGV)-causing serovars of Chlamydia trachomatis in a biological or non-biological sample. The methods can include performing an amplification step, a hybridization step, and a detection step. Furthermore, oligonucleotide primers and probes targeting the pmpH gene for the L serovars of Chlamydia trachomatis, along with kits are provided that are designed for the detection of L serovars.

IPC Classes  ?

• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

The present invention provides novel primers in which a ferrocene label is attached to the primer. The ferrocene label is incorporated into the amplification product. When the amplification product incorporating the ferrocene label is denatured, it can bind to a capture probe and the presence or absence of the ferrocene label can be detected via electrochemical detection. The system avoids the use of a signal probe in a sandwich assay as historically used during electrochemical detection.

IPC Classes  ?

• C12Q 1/6816 - Hybridisation assays characterised by the detection means
• C12Q 1/6825 - Nucleic acid detection involving sensors

Abstract

A method for autonomous teach-in of at least one target position of a supply device (120) of a slide imaging apparatus (110) is disclosed. The slide imaging apparatus (110) comprises at least one imaging device (126, 128) configured to generate an image of a sample mounted on a slide (140). The target position is a position on the imaging device (126, 128). The slide imaging apparatus (110) comprises at least one operating system (170) configured for controlling operation of the supply device (120). The method comprises the following steps i) (186) providing at least six pre-defined positions by using the operating system (170); ii) (188) driving the supply device (120) to the six pre-defined positions until colliding with the imaging device (126, 128) by using the operating system (170) and detecting collisions with the imaging device (126, 128); iii) (190) evaluating the detected collisions by using the operating system (170), thereby determining the target position.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• B25J 9/16 - Programme controls
• G05B 19/425 - Teaching successive positions by numerical control, i.e. commands being entered to control the positioning servo of the tool head or end effector
• B25J 13/08 - Controls for manipulators by means of sensing devices, e.g. viewing or touching devices
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system

Abstract

Techniques described herein include, for example, generating a feature map for an input image, generating a plurality of concentric crops of the feature map, and generating an output vector that represents a characteristic of a structure depicted in a center region of the input image using the plurality of concentric crops. Generating the output vector may include, for example, aggregating sets of output features generated from the plurality of concentric crops, and several methods of aggregating are described. Applications to classification of a structure depicted in the center region of the input image are also described.

IPC Classes  ?

• G06V 10/44 - Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersections; Connectivity analysis, e.g. of connected components
• G06T 7/00 - Image analysis
• G06V 10/82 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks
• G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts

Abstract

A machine learning and/or deep learning framework was constructed and used to forecast COVID-19 cases and deaths. Multiple open data sources relevant for the pandemic evolution in a geographic area, such as the United States or another country or region, can be processed to extract a plurality of features, such as localized (i.e., county level, city level, regional level, province level, etc.) COVID cases and deaths, demographics and socioeconomic factors, non- medical interventions, and mobility (i.e., from cell phone and/or GPS data).

IPC Classes  ?

• G16H 50/80 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for detecting, monitoring or modelling epidemics or pandemics, e.g. flu

Abstract

A slide carrier includes: a base support; and a slide platform having a surface that is parallel to a first plane defined by a first vector and a second vector, wherein a vector extending in a direction opposite to the direction of gravity is normal with respect to a second plane defined by a third vector and a fourth vector, an angle between the first vector and the third vector is greater than zero degrees and less than 90 degrees, and an angle between the second vector and the fourth vector is greater than zero degrees and less than 90 degrees.

IPC Classes  ?

• B01L 9/00 - Supporting devices; Holding devices
• B25J 15/00 - Gripping heads
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

Disclosed herein are techniques for facilitating a clinical decision for a patient based on identifying a group of patients having similar attributes as the patient. The group of patients can be identified using information from a predictive machine learning model that performs a clinical prediction for the patient. At least some of the attributes of the group of patients can be output to support a clinical decision. The attributes may include, for example, biography data of the patient, results of one or more laboratory tests of the patient, biopsy image data of the patient, molecular biomarkers of the patient, a tumor site of the patient, and a tumor stage of the patient.

IPC Classes  ?

• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients

Abstract

The present invention relates to methods to improve detection of amplification products from Loop-mediated Amplification (LAMP) reactions by the use of nucleotide analogs that form duplex structures that nucleic acid polymerase enzymes do not recognize efficiently.

IPC Classes  ?

• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates

Abstract

Automated methods for genotyping melting curves are described. Melting curves are processed and difference matrices are compiled based on the processed matrices. The difference matrices are used to compile a cluster matrix and the cluster matrix is filtered to determine clusters that are used for genotyping the melting curves.

IPC Classes  ?

• G16B 25/10 - Gene or protein expression profiling; Expression-ratio estimation or normalisation
• G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
• G16B 40/30 - Unsupervised data analysis
• C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism

Abstract

A computer-implemented method for performing a clinical prediction is disclosed. The method comprises the following steps: i) (110) retrieving input data via at least one communication interface (164) of a processing device (166), wherein the input data comprises multiple different modalities of a patient; ii) (114) processing the input data by using the processing device (166), wherein the processing comprises generating embedding modality representations from the input data by using at least one trainable data embedder, wherein the processing comprises combining the embedding modality representations using at least one aggregation network thereby generating the clinical prediction, wherein the aggregation network comprises at least one attention layer and/or at least one transformer layer; and iii) (118) generating an output of the clinical prediction by using the processing device (166).

IPC Classes  ?

• G06N 3/04 - Architecture, e.g. interconnection topology
• G16H 30/00 - ICT specially adapted for the handling or processing of medical images
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients
• G16H 20/00 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance
• G06N 3/045 - Combinations of networks
• G16H 30/40 - ICT specially adapted for the handling or processing of medical images for processing medical images, e.g. editing

Abstract

The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying positively charged tags from oligonucleotide probes, bound to target nucleic acids, are produced during PCR by the 5`-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique positively charged tags.

IPC Classes  ?

• C12Q 1/6823 - Release of bound markers
• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/6872 - Methods for sequencing involving mass spectrometry

Abstract

A machine learning model for traversing a decision tree, the machine learning model trained from a structured data set including a first set of key-value pairs and subject-specific criteria using the key-value pairs. The first set of key-value pairs is transformed into a second set of key-value pairs, which are projected to a subject-specific point within a multi-dimensional space. The decision tree includes decision and leaf nodes. Each leaf node is connected to a root node via a leaf-node-specific trajectory. Each decision node corresponds to a criterion using a value in the second set of key-value pairs. For each leaf node, a leaf-node-specific point within the multi-dimensional space is determined using the leaf-node-specific trajectory, and a similarity score is determined using the leaf-node-specific and subject-specific points. A subset of the leaf nodes is identified using the scores. State or protocol information for each leaf node in the subset is retrieved.

IPC Classes  ?

• G06N 5/022 - Knowledge engineering; Knowledge acquisition
• G06N 20/00 - Machine learning

Abstract

A method for controlling a laboratory system or device comprising at least one pipettor with a pressure sensor, at least one humidity sensor, at least one temperature sensor, and a control unit. The control unit receiving a humidity value from the humidity sensor and a temperature value from the temperature sensor, comparing the pair of humidity and temperature values with a threshold database connected to the control unit, the threshold database comprising, for different pairs of humidity and temperature values, instructions to activate or deactivate an anti droplet control system of the pipettor, determining if the anti droplet system of the pipettor has to be activated or deactivated, activating or deactivating said anti droplet system of the pipettor.

IPC Classes  ?

• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

A laboratory system or device, comprising a reagent storage area (1) comprising at least two reagent drawers (2, 3) mounted slidably between an open position (O) and a closed position (C), each of the reagent drawers comprising receptacles for a plurality of reagent cartridges (RC1, RC2), and at least two locking mechanisms (4, 5) for respectively locking/unlocking the at least two reagent drawers (2, 3) when in the closed position (C), at least one robotic handler (6) and a control unit (7), wherein the reagent storage area (1) is subdivided in a loading zone (LZ), a storage zone (SZ) and a pipetting zone (PZ), and the reagent storage area (1) further comprises a handling zone (HZ).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

vanA vanBvanAvanBvanAvanBvanB.

IPC Classes  ?

• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

A laboratory system or device comprising a control unit for controlling operation of the system or device, and a storage unit connected to the control unit and containing instruction for each assay type of the control type to be used when running said assay type, and, for each control type, an allocation rule for the control type. The control unit receiving at least one assay order comprising instructions to run at least one assay type on the system or device, determining for the at least one assay type comprised in the at least one assay order, the control type to be used, and the corresponding allocation rule for said control type, scheduling an assay run comprising instructions to include the determined control type based on the determined allocation rule, and controlling the system or device to perform the scheduled assay run.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

A method for optimizing handlers operation in a laboratory system or device comprising at least two handlers movably arranged within the system or device in a shared area, and a control unit for controlling the operation of the at least two handlers comprising the following steps: defining for each of the handlers a task, receiving a first operation schedule, the operation schedule comprising a sequence of task to be performed by the handlers, starting operation of the handlers according to the received operation schedule, receiving a second operation schedule, creating an updated operation schedule, and continuing operation of the handlers according to the updated operation schedule.

IPC Classes  ?

• G16H 40/60 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices
• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis

Abstract

A method for increasing operational security of a laboratory system or device comprising: a reagent storage area (1) comprising at least two reagent drawers (2, 3) mounted slidably along a first axis Y between an open position (O) and a closed position (C) and arranged stacked with respect to a second axis Z perpendicular to the first axis Y, and having each a position sensor (P2, P3) for determining the closed position (C) of the respective reagent drawer (2, 3), at least one robotic handler head (4) movably arranged in the reagent storage area at least along the first axis Y and the second axis Z a control unit (5) configured to control the operation of the robotic handler head (4) and connected to the position sensors (P2, P3), the method comprising the steps of defining for each reagent drawer (2, 3) a first forbidden zone along the first axis Y (Y) and a second forbidden along the second axis Z (Z2, Z3), determining by means of the position sensors (P2, P3) if the reagent storage drawers (2, 3) are in the closed position (C), and control the operation of the handler head (4).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

A method for controlling an anti droplet system of a laboratory system or device comprising at least one air displacement pipettor with a pressure sensor and a control unit for controlling operation of the pipettor, the method comprising the following steps controlling the pipettor to be moved such that the pipettor tip is immerged in a fluid to be aspirated, aspirate a predetermined volume of fluid, move the pipettor such that the pipettor tip is emerged from the fluid, continuously monitoring the pressure above the fluid column in the pipettor tip and generating pressure curve over time, and determining if a pressure increase above the fluid column has been detected.

IPC Classes  ?

• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

An instrument (110) for automatically dissecting a biological specimen (112) on a slide (114) is proposed. The instrument (110) comprises at least one imaging system (116). The imaging system (116) comprises at least one camera (118) configured for sequentially imaging at least one image of the slide (114) at a plurality of slide positions. The imaging system (116) com- prises a relay lens system (120) having a fixed focal length. The relay lens system (120) is configured for relaying an impinging light beam (122) from the slide (114) to the camera (118). The instrument (110) further comprises a movable xy-stage (150) configured for setting the slide (114) position. The instrument (110) further comprises at least one processing unit (162) configured for generating a full slide (114) image by stitching the sequentially imaged images of the slide (114). Further, a method for automatically dissecting a biological specimen (112) on a slide (114) is proposed.

IPC Classes  ?

• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• G02B 21/26 - Stages; Adjusting means therefor
• G02B 21/24 - Base structure

Abstract

Methods for the rapid detection of the presence or absence of Malaria parasites (including Plasmodium) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Additionally, the assay can be a multiplex assay, to amplify and detect a plurality of Plasmodium targets simultaneously, which offers advantages over singleplex assays. Furthermore, primers and probes targeting Malaria parasites (including Plasmodium) and kits are provided that are designed for the detection of Plasmodium, including, but not limited to, the Plasmodium species of P. falciparum, P. vivax, P. ovale, P. knowlesi, and P. malariae. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of Malaria parasites (including Plasmodium).

IPC Classes  ?

• C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Abstract

In some embodiments, methods, systems, software and uses are provided for synchronizing medical images displays. An input identifying a region of interest in a first medical image is received. A second region of interest in a second medical image is determined based the first region. The first medical image and a first indication of the first region are displayed in a first viewport of a GUI. The second medical image and a second indication of the second region are displayed in a second viewport of the GUI. A display adjustment input is received to adjust the displaying of one of the first or second region of interest. Based on the display adjustment input and the correspondence information, an adjustment of the displaying of the first region of interest in the first viewport and an adjustment of the displaying of the second region of interest in the second viewport is implemented.

IPC Classes  ?

• G16H 30/20 - ICT specially adapted for the handling or processing of medical images for handling medical images, e.g. DICOM, HL7 or PACS
• G06N 3/08 - Learning methods
• G06T 7/00 - Image analysis
• G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems

Abstract

The present invention describes methods for temperature multiplexed PCR for detection and quantitation of target nucleic acids with increased sensitivity. The methods are performed by contacting the sample with probes designed for temperature multiplexed PCR, measuring the fluorescence at multiple temperatures, subtracting signals to obtain calculated signals for each target of interest. Furthermore, the methods comprise obtaining a baseline of the first fluorescent signal, and adjusting the calculated signals using the obtained baseline of the first fluorescent signal to obtain a baseline shifted calculated signals.

IPC Classes  ?

• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

In one embodiment, a method includes indexing a whole slide image dataset to generate one or more dataset embeddings corresponding to one or more respective regions of one or more whole slide images. Each dataset embedding includes a feature vector mapping the respective region to a feature embedding space. The method includes accessing a query image and generating an embedding for the query image that includes a feature vector mapping the query image to the feature embedding space. The method includes identifying result tiles by comparing the embedding for the query image to one or more of the dataset embeddings. The comparison is based on a distance between the embedding for the query image and the one or more of the dataset embeddings in the feature embedding space. The method includes generating a user interface including a display of the result tiles

IPC Classes  ?

• G06F 16/532 - Query formulation, e.g. graphical querying
• G06F 16/51 - Indexing; Data structures therefor; Storage structures
• G06F 16/56 - Information retrieval; Database structures therefor; File system structures therefor of still image data having vectorial format
• G06T 7/00 - Image analysis
• G06V 10/44 - Local feature extraction by analysis of parts of the pattern, e.g. by detecting edges, contours, loops, corners, strokes or intersections; Connectivity analysis, e.g. of connected components
• G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
• G16H 30/20 - ICT specially adapted for the handling or processing of medical images for handling medical images, e.g. DICOM, HL7 or PACS
• G16H 30/40 - ICT specially adapted for the handling or processing of medical images for processing medical images, e.g. editing
• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients

Abstract

Techniques for patient data management include obtaining medical history data of patients from a database, the medical history data including a history of one or more diagnosis events, one or more treatment events, and a clinical outcome event for each patient of the patients. For each patient, based on the medical history data, a computing system generates a patient pathway that includes a graph including nodes of one or more diagnosis events, the one or more treatment events, and the clinical outcome event. The computing system receives, via an interface, one or more criteria to select a subset of the patient pathways of the patients. The computing system selects, based on the one or more criteria, graphs representing the subset of the patient pathways. The computing system aggregates the subset of the patient pathways into a merged graph. The computing system displays the merged graph in the interface.

IPC Classes  ?

• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records

Abstract

Methods for the rapid detection of the presence or absence of Hepatitis Delta Virus (HDV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the HDV Ribozyme domain and the HDV Hepatitis Delta Antigen (HDAg) gene, along with kits are provided that are designed for the detection of HDV.

IPC Classes  ?

• C12Q 1/6851 - Quantitative amplification
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

Methods for the rapid detection of the presence of variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that contain mutations in the Spike (S) protein gene in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting SARS-CoV-2 variants containing S gene mutations and kits are provided that are designed for the detection of SARS-CoV-2 variants containing S gene mutations, in particular the 69-70 deletion, the N501Y and the 484K mutations. Also claimed are specific methods for performing allele-specific amplification employing blocking probes with at least one nucleotide which is an LNA nucleotide.

IPC Classes  ?

• C12Q 1/6858 - Allele-specific amplification
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

Methods for the rapid detection of the presence or absence of Human Parainfluenza Viruses (HPIV), including HPIV 1-4 in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting HPIV 1-4, and kits are provided that are designed for the detection of target regions of HPIV 1-4. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of HPIV 1-4.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

Systems and methods are provided for managing patient data. The system integrates medical data from multiple sources to a unified patient database. Structured and unstructured medical data is obtained, enriched (e.g., by designating data field types, standardizing data types or terminology, and the like), and stored to the unified patient database. The data retrieved from the disparate sources is stored to data elements in the unified patient database in a network of connected objects including data about tumor masses, treatments, reports, medical history, and diagnoses. The data in the unified patient database is used to display patient data in user-friendly interface views, including a patient journey view that displays patient data in a chronological fashion organized by data types. The different interface views can be traversed to display patient data originating from disparate sources with ease, to improve the clinical decision making process.

IPC Classes  ?

• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records

Abstract

CandidaCandidaCandida.

IPC Classes  ?

• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

The present invention allows for the expansion of multiplexing capabilities of common PCR devices by using fluorogenic PCR probes made of large Stokes shift (LSS) fluorescent dyes. With this approach, no changes of the hardware or software components in the instrument are required.

IPC Classes  ?

• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

The present invention relates to methods for the specific lysis of eukaryotic cells in a sample containing microorganisms such as bacterium and fungus by incubating the sample in a buffer of around pH 9 that contains a non-ionic detergent.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Methods for the rapid detection of the presence or absence of Malaria parasites (including Plasmodium ) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Additionally, the assay can be a multiplex assay, to amplify and detect a plurality of Plasmodium targets simultaneously, which offers advantages over singleplex assays. Furthermore, oligonucleotide primers and oligonucleotide probes targeting Malaria parasites (including Plasmodium) and kits are provided that are designed for the detection of Plasmodium, including, but not limited to, the Plasmodium species of P. falciparum, P. vivax, P. ovale, P. knowlesi, and P. malariae. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of Malaria parasites (including Plasmodium).

IPC Classes  ?

• C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Abstract

The invention relates to a method for managing information dealing with a medical test for assessing a predetermined medical condition by means of a distributed system (100) comprising a plurality of communication devices (210, 312, 322, 410), the method comprising obtaining by a user communication device (210) information on an access code provided in a test-kit for assessing a predetermined medical condition, the user communication device (210) being configured for receiving and displaying messages addressed to a unique identifier. Further, the method comprises providing instructions relating to analysis of the medical sample. A negative analysis result is communicated to the user communication device (210) of the user. In case of a positive analysis result, consultation by a medical entity is established. Further aspects relate to a user communication device, a computer program product, a central server and a medical test-kit for use with the proposed method.

IPC Classes  ?

• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
• G16H 40/60 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices
• G16H 80/00 - ICT specially adapted for facilitating communication between medical practitioners or patients, e.g. for collaborative diagnosis, therapy or health monitoring
• G16H 50/80 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for detecting, monitoring or modelling epidemics or pandemics, e.g. flu
• G01N 33/487 - Physical analysis of biological material of liquid biological material
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

A computer-implemented method for quality control of at least one digital image of a sample (112) mounted on a slide (114) is proposed. The method comprises the following steps: a) Providing at least one digital image of the sample (112) mounted on the slide (114) using at least one imaging device (116) of a slide imaging apparatus (110); b) Determining quality of the digital image by determining sharpness values of sub- regions of at least one region of interest of the digital image by using at least one edge detection image filter and comparing the sharpness values within the region of interest, wherein the quality of the region of interest is classified depending on the comparison; c) Generating at least one indication depending on the classification of the quality, wherein steps b) and c) are performed automatically.

IPC Classes  ?

• G06T 7/00 - Image analysis

Abstract

A device for mixing fluid media. The device is a bladeless mixer. The device comprises a hollow body 1 with at least a single inlet opening in its bottom and at least a single outflow region in its side. The device is rotated at a speed at which the media due to centrifugal forces enters the device's hollow cavity via the inflow opening or unit. The media is discharged from the cavity through one or more outlet units or openings in the sides or in the top of the device. During mixing the media does not rise above the level of the undisturbed media.

IPC Classes  ?

• B01F 7/00 - Mixers with rotary stirring devices in fixed receptacles; Kneaders
• B01F 7/28 - Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a vertical axis with cylinders

Abstract

Methods for the rapid detection of the presence or absence of SARS-CoV-2 in biological or non-biological samples are described. These methods are adapted to be performed rapidly in a point-of-care setting. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Specifically, primers and probes targeting the orf1ab gene or the N gene of SARS-CoV-2 are provided that are designed for the detection of this target. Additionally, kits and reaction vessels containing primers and probes targeting SARS-CoV-2 are provided. Additionally, methods, kits and reaction vessels for the simultaneous rapid detection of the presence or absence of SARS-CoV-2, influenza A, and influenza B in biological or non-biological samples are described.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

In some examples, a digital antimicrobial stewardship program (ASP) system includes an ASP team sub-system and a treating team sub-system. The ASP team sub-system can provide the ASP team access to relevant information for a clinical decision to intervene the prescription of antibiotics and/or a diagnostic test order by the treating team, and transmit an intervention recommendation to the treating team sub-system. The ASP team sub-system can also provide a recommendation for the intervention recommendation. The treating team sub-system can provide the treating team access to relevant information for a clinical decision to prescribe antibiotics or a diagnostic test to a patient. The treating team sub-system can also generate a recommendation for the prescription. The treating team sub-system can also receive the intervention recommendation from the ASP sub-system, and display the intervention recommendation and the information on a mobile device.

IPC Classes  ?

• G16H 10/20 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for electronic clinical trials or questionnaires
• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
• G16H 20/10 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance relating to drugs or medications, e.g. for ensuring correct administration to patients
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems

Abstract

An event chain reaction system (128) is disclosed. The event chain reaction system (128) comprises: - at least one communication interface (156) configured for receiving at least one event stream (160), wherein the event stream (160) comprises at least one sequence of ordered events generated by at least one analytical system (112), wherein each event comprises information about a change in a state of the analytical system (112) and/or any of loaded resources; - at least one chain reaction component (158) comprising at least one chain matching element (162), wherein the chain matching element (162) is configured for recognizing at least one chain on the event stream (160), wherein the chain comprises a set of ordered events to be searched, wherein a first event of the chain defines a start event (166), wherein the chain matching element (162) is configured for identifying the start event (166) in the event stream (160) and, upon identifying the start event (166), the chain matching element (162) is configured for successively determining whether the other events of the chain match to one of the events of the event stream (160), wherein, in case all events of the chain are matched to events of the event stream (160), the chain matching element (162) is configured for triggering at least one reaction, wherein the reaction comprises generating information that a chain was matched and/or issuing a command to at least one component of the analytical system (112), wherein, in case one of the events of the chain is not matched, the chain matching element (162) is configured for resetting to its initial state and waiting for the start event (166). Further, a system (110) for monitoring and/or controlling, a computer implemented method and a computer program for determining at least one feature of at least one component of an analytical system (112), a computer implemented method and a computer program for monitoring and/or controlling at least one feature of at least one component of an analytical system (112) are disclosed.

IPC Classes  ?

• G06F 9/54 - Interprogram communication
• G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
• G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
• G16H 40/67 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for remote operation
• G05B 23/02 - Electric testing or monitoring

Abstract

A method of verifying multi-modal medical data is proposed. The method comprises: accessing multi-modal medical data of a subject, the multi-modal medical data comprising a medical image of a specimen slide, wherein a specimen in the specimen slide was collected from the subject; generating a prediction pertaining to a biological attribute of the medical image based on the medical image; determining a degree of consistency between the biological attribute of the medical image and other modalities of the multi-modal medical data; and outputting, based on the degree of consistency, an indication of whether the multi-modal medical data contain inconsistency.

IPC Classes  ?

• G16H 30/40 - ICT specially adapted for the handling or processing of medical images for processing medical images, e.g. editing
• G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
• G06N 3/04 - Architecture, e.g. interconnection topology
• G06T 7/00 - Image analysis
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records

Abstract

A method comprises: receiving data corresponding to a plurality of data categories of a patient; selecting, from a plurality of trained machine learning models and based on the plurality of data categories, a first machine learning model and a second machine learning model, the first machine learning model being trained using first data of a first subset of the plurality of data categories and having a first weight indicative of a first performance metric value, the second machine learning model being trained using second data of a second subset of the plurality of data categories and having a second weight indicative of a second performance metric value; generating a first prediction result and a second prediction result using, respectively, the first model and the second model; and generating a combined prediction result based on the first prediction result, the second prediction result, the first weight and the second weight.

IPC Classes  ?

• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients
• G06N 20/20 - Ensemble learning

Abstract

A computer-implemented method is provided. The method comprises: receiving, from a text field of a user interface, input text strings containing medical data; identifying, based on language semantics and grammatical structure, a keyword and one or more data values from the input text strings; providing the keyword as an input to a query of a medical data category database to obtain one or more categories associated with the keyword, the one or more categories including a first category; retrieving, from a template database, a first template associated with the first category, the first template including one or more fixed blocks and one or more variable blocks; inserting a data value corresponding to the keyword into a variable block of the first template to generate a replacement text string; and displaying the replacement text string in place of the input text strings in the text field.

IPC Classes  ?

• G16H 10/20 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for electronic clinical trials or questionnaires
• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
• G16H 15/00 - ICT specially adapted for medical reports, e.g. generation or transmission thereof

Abstract

The present disclosure relates to a slide imaging apparatus (110) and to a method (210) for imaging a plurality of slides (140). Herein, the slide imaging apparatus (110) comprises: - at least one imaging device (126, 128) configured to generate an image (162) of a sample mounted on a slide (140), wherein the imaging device (126, 128) comprises at least one operating button (160); - a storage device (118) loadable with a plurality of slides (140) and configured to store the slides (140); and - a supply device (120) configured to supply the slides (140) from the storage device (118) to the imaging device (126, 128), wherein the supply device (120) is configured to press the operating button (160). The slide imaging apparatus (110) and the method (210) for imaging a plurality of slides (140) enable improved processing of the slides to be processed in an imaging device, wherein the slides are provided to be introduced into a slide reception of an imaging device.

IPC Classes  ?

• G02B 21/24 - Base structure
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
• G01N 21/00 - Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light

Abstract

The present disclosure relates to a slide imaging apparatus (110) and to a method (210) for imaging a plurality of slides (140). Herein, the slide imaging apparatus (110) comprises: - at least one first imaging device (126, 126') and at least one second imaging device (128, 128'), each configured to generate an image (162) of a sample mounted on a slide (140); - a storage device (118) loadable with a plurality of slides (140) and configured to store the slides (140); and - a supply device (120) configured to selectively supply the slides (140) from the storage device (118) to the at least one first imaging device (126, 126') or to the at least one second imaging device (128, 128'), wherein the at least one first imaging device (126, 126') and the at least one second imaging device (128, 128') comprise at least one visual indicator configured to indicate an operational status of the at least one first imaging device (126, 126') and the at least one - second imaging device (128, 128'), wherein the slide imaging apparatus (110) further comprises at least one vision sensor (166) configured to detect an operational status of the at least one first imaging device (126, 126') and of the at least one second imaging device (128, 128') using the at least one visual indicator. The slide imaging apparatus (110) and the method (210) for imaging a plurality of slides (140) enable improved processing of the slides (140) in an event of a failure of any one of the imaging devices (126, 128; 126', 128'), wherein the improved processing of the slides (140) avoids a manual handling of the slides (140) in the event of the failure of any one of the imaging devices (126, 128; 126', 128').

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• G02B 21/24 - Base structure
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides

Abstract

Methods for the rapid detection of the presence or absence of SARS-CoV-2, influenza A and influenza B in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting SARS-CoV-2, influenza A, and influenza B and kits are provided that are designed for the detection of SARS-CoV-2, influenza A and influenza B.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

The present invention relates to methods and compositions for performing quantitative polymerase chain reaction (qPCR) to screen for previously undiscovered inducible prophages from various bacterial strains. The present invention also relates to methods for performing qPCR to identify inducible prophages for creation of functional non-replicative transcription particles (NRTPs).

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

Methods for the rapid detection of the presence or absence of mecA- and/or mecC-containing Staphylococcus aureus (mecA/mecC-MRSA) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the genes for mecA-MRSA and mecC-MRSA, along with kits are provided that are designed for the detection of mecA/mecC-MRSA.

IPC Classes  ?

• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

The present invention is directed to a foldable transport container suitable to receive and hold multiple plates with samples in it. The container is comfortable to load, transport and unload. The invention also relates to a strip of material for providing such a foldable transport container. Finally the invention relates to a method for assembling such a foldable container.

IPC Classes  ?

• B01L 9/00 - Supporting devices; Holding devices
• B65D 5/20 - Rigid or semi-rigid containers of polygonal cross-section, e.g. boxes, cartons or trays, formed by folding or erecting one or more blanks made of paper by folding-up portions connected to a central panel from all sides to form a container body, e.g. of tray-like form
• B65D 5/30 - Rigid or semi-rigid containers of polygonal cross-section, e.g. boxes, cartons or trays, formed by folding or erecting one or more blanks made of paper by folding-up portions connected to a central panel from all sides to form a container body, e.g. of tray-like form with tongue-and-slot or like connections between sides and extensions of other sides
• B65D 5/42 - Rigid or semi-rigid containers of polygonal cross-section, e.g. boxes, cartons or trays, formed by folding or erecting one or more blanks made of paper - Details of containers or of foldable or erectable container blanks
• B65D 5/46 - Handles

Abstract

The present invention provides for water-soluble mono- and dicationic fluorescent dyes with the latter exhibiting stable fluorescence at elevated temperatures. The present invention also provides for methods for the production of the fluorescent dyes and for using these dyes in biological assays such as multiplexing qPCR and tissue staining.

IPC Classes  ?

• C09B 1/00 - Dyes with an anthracene nucleus not condensed with any other ring
• C07F 7/08 - Compounds having one or more C—Si linkages
• C09B 11/28 - Pyronines
• G01N 33/533 - Production of labelled immunochemicals with fluorescent label

Abstract

The present invention relates to compositions and methods for the use of polymerase chain reaction (PCR) as a reporter assay for rapid and simultaneous bacterial identification and phenotype testing for antimicrobial susceptibility (AST). The current invention uses a strategy that has shown the ability for multiplexing and for handling polymicrobial samples for antimicrobial susceptibility testing.

IPC Classes  ?

• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

Methods and systems for providing an interactive guideline are provided. In one example, a method comprises: receiving, from a database, data of a directed graph representing a medical guideline, the medical guideline including a decision tree including a plurality of clinical decisions and preconditions leading to at least some of the clinical decisions, the directed graph including a plurality of nodes representing the clinical decisions and the preconditions and edges connecting the plurality of nodes to represent dependency relationships among the clinical decisions and the preconditions; providing, via a navigation interface, a graphical representation of at least part of the directed graph; receiving, via the navigation interface, a selection of a node of the directed graph from the graphical representation; and based on the selection of the node, updating the graphical representation of the at least part of the directed graph to provide a navigation result of the medical guideline.

IPC Classes  ?

• G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
• G16H 70/20 - ICT specially adapted for the handling or processing of medical references relating to practices or guidelines

Abstract

In one example, a method being performed by a computer system comprises: receiving an image file containing a pathology report; performing an image recognition operation on the image file to extract input text strings; detecting, using a natural language processing (NLP) model, entities from the input text strings, each entity including a label and a value; extracting, using the NLP model, the values of the entities from the input text strings; converting, based on a mapping table that maps entities and values to pre-determined terminologies, the values of at least some of the entities to the corresponding pre-determined terminologies; and generating a post-processed pathology report including the entities detected from the input text strings and the corresponding pre-determined terminologies.

IPC Classes  ?

• G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
• G16H 15/00 - ICT specially adapted for medical reports, e.g. generation or transmission thereof
• G16H 10/00 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data
• G06F 40/279 - Recognition of textual entities

Abstract

Methods for the rapid detection of the presence or absence of Hepatitis B Virus (HBV) RNA in a biological or non-biological sample are described, wherein competitive blocking oligonucleotides prevent amplification of HBV DMA. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, competitive blocking oligonucleotides for preventing amplification of HBV DMA, primers and probes for targeting HBV HBV (in particular HBV RNA transcribed from cccDNA, such as pgRNA) and kits are provided that are designed for the detection of HBV HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA.

IPC Classes  ?

• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

Methods for the rapid detection of the presence or absence of Epstein Barr Virus (EBV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting EBV, and kits are provided that are designed for the detection of target regions of EBV. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of EBV. Also described are primers and probes that detect different regions of EBV, and can be employed in a dual target assay for simultaneously detecting two different and non-overlapping target regions of EBV.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Abstract

The present invention relates to the use of peptidimimetic antimicrobial peptides as additives in non-replicative transduction particles based systems to either limit cross-reactivity of unwanted organisms or to identify the organism being run on an antibiotic susceptibility assay (AST assay). Addition of the peptides removes or reduces light production from bacteria that are sensitive to them, allowing for prevention of cross-reactivity in AST assays and/or family, genus, and potentially species level bacteria strain identification when performing AST testing.

IPC Classes  ?

• C12N 7/00 - Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• C12N 15/86 - Viral vectors
• C12Q 1/10 - Enterobacteria
• C12Q 1/18 - Testing for antimicrobial activity of a material
• C12Q 1/66 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
• C12Q 1/6897 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

Methods for the rapid detection of the presence or absence of NG Pilin Inverting protein (PivNg) gene in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the NG PivNg genes, along with kits are provided that are designed for the detection of Neisseria gonorrhoeae (NG).

IPC Classes  ?

• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

Methods for preferentially and/or selectively detecting and/or quantitating target RNA, over DNA, in a biological or non-biological sample are described. The methods include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the reaction mixture includes dNTPs, that include dITP. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the preferential and/or selective amplification and detection and/or quantitation of target RNA (over DNA), in the presence of dITP. The methods, kits, and reaction mixtures are for the preferential and/or selective detection and/or quantitation of RNA, over DNA, and are particularly useful with samples that comprise a number of different types of nucleic acids (e.g., DNA and RNA).

IPC Classes  ?

• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Abstract

In one example, a method of extracting patient information for a medical application comprises: receiving patient data of a patient; processing the patient data using a learning system with Artificial Intelligence (AI)-assisted clinical extraction tool, the processing comprising: extracting, based on a trained language extraction model that reflects language semantics and a user's prior habit of entering other patient data, data elements from the patient data and data categories represented by the data elements, and mapping at least some of the extracted data elements to pre-determined data representations based on the data categories; populating fields of a data record of the patient based on the pre-determined data representations; and storing the populated data record in a database accessible by the medical application.

IPC Classes  ?

• G16H 10/60 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for patient-specific data, e.g. for electronic patient records
• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems

Abstract

The present invention relates to compositions comprising and methods of producing genetically engineered bacteriophages, bacteriophage-like particles and non-replicating transduction particles (NRTPs) that contain non-native tail fibers that display altered host specificity and/or reactivity. The present invention also relates to methods of using these bacteriophages and NRTPs for the development of novel diagnostics, therapeutics and/or research reagents for bacteria-related diseases.

IPC Classes  ?

• C12N 7/00 - Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
• C07K 14/005 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
• C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
• C12Q 1/66 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

The present invention relates to the use of sideromycins as additives in non-replicative transduction particles based systems to either limit cross-reactivity of unwanted organisms or to identify the organism being run on an antibiotic susceptibility assay (AST assay). Addition of the sideromycins removes or reduces light production from bacteria that are sensitive to them, allowing for prevention of cross-reactivity in AST assays and/or family, genus, and potentially species level bacteria strain identification when performing AST testing.

IPC Classes  ?

• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
• C12Q 1/6897 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

Acinetobacterbaumannii (A. baumannii)Abaumannii.baumannii.

IPC Classes  ?

• C12Q 1/6897 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• C12N 7/00 - Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
• C12Q 1/18 - Testing for antimicrobial activity of a material

Abstract

The present invention describes methods for performing real-time PCR for detection and quantitation of target nucleic acids using tagged oligonucleotide probes and solid-phase molography.

IPC Classes  ?

• C12Q 1/6825 - Nucleic acid detection involving sensors
• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates

Abstract

Candida aurisCandida auris (CA) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the CA 5.8s/ITS2 rRNA gene, along with kits are provided that are designed for the detection of CA.

IPC Classes  ?

• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

In one example, an apparatus comprises a treatment metric model database that stores a plurality of treatment metric models, each of the plurality of treatment metric models being generated for a first cluster of cluster features vectors, each cluster feature vector representing a distribution for each of a plurality of data categories of patients data represented by patient feature vectors clustered in a second cluster. The apparatus is further configured to: receive patient characteristics features data of a patient; identify a first treatment metric model based on the patient characteristics features data of the patient; input the first patient characteristic features data and data representing a range of administrations of the treatment to the first treatment metric model to compute a range of the treatment metric; and select an administration of the treatment from the range of administrations of the treatment having optimal treatment metric.

IPC Classes  ?

• G16H 50/70 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients

Abstract

Methods and systems for performing a clinical prediction are provided. In one example, the method comprises: receiving first molecular data of a patient; receiving first biopsy image data of the patient; processing, using a machine learning model, the first molecular data and the first biopsy image data to perform a clinical prediction, wherein the machine learning model is generated or updated based on second molecular data and second biopsy image data of a plurality of patients; and generating an output of the clinical prediction.

IPC Classes  ?

• G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
• G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
• G16B 25/00 - ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
• C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Abstract

Disclosed are DNA polymerases having increased 5'-3' strand displacement activity and substantially reduced 5'-3' exonuclease and endonuclease activity relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12Q 1/6869 - Methods for sequencing
• C12N 15/09 - Recombinant DNA-technology

Abstract

Disclosed herein are nanopore sequencing devices which include electrodes comprising ruthenium-containing materials, such as ruthenium containing materials having a double layer capacitance ranging from between about 180 pF/um2to about 320 pF/um2. In addition methods of manufacture of such materials and electrodes are disclosed.

IPC Classes  ?

• G01N 33/487 - Physical analysis of biological material of liquid biological material
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

Disclosed is a method and laboratory instrument aimed at reducing the loss of time of liquid level determination each time a liquid container is loaded into an instrument and at the same time reducing waste of liquid containers due to unforeseen changes in the liquid levels by combining a fast liquid level check at an expected liquid level with a liquid level search if no liquid level is found at the expected level, while also ensuring proper liquid aspiration as the liquid level is always verified (expected level is not blindly relied upon).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

The present invention relates to compositions and methods (reagents and protocols) for the post-synthetic modification of nucleic acids obtained from solid-phase oligonucleotide synthesis with a label (such as fluorescent dyes). The coupling reagent is the triazine-based salt 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMT-MM) in the presence of a counteranion.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12N 15/11 - DNA or RNA fragments; Modified forms thereof
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical

Abstract

A cartridge for determining a concentration of target cells within a sample includes a separation portion and a detection portion. The separation portion includes a first and second surface defining a separation chamber. The separation portion can contain a density medium having a density greater than a density of a first portion of the sample and less than a density of a second portion of the sample (that includes the target cells). The separation chamber can be fluidically coupled to an inlet reservoir such that the sample can pass from the inlet reservoir to the separation chamber during rotation. The detection portion includes a detection surface that forms a boundary of a detection chamber. The detection surface is nonparallel to the first surface such that the target cells impinge on the detection surface when passing into the detection chamber. The detection surface is configured to capture the target cells.

IPC Classes  ?

• G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/569 - Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises fluorinated nucleoside polyphosphates at the terminal phosphate, e.g. at the gamma-phosphate. The present invention also provides for methods for using the fluorinated nucleoside polyphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

IPC Classes  ?

• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

e.g.e.g., for production of the DNA polymerases. Further disclosed are kits and reaction mixtures comprising the improved DNA polymerases as well as methods of primer extension using the improved DNA polymerases.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Abstract

Provided herein are improved methods and compositions for detecting microsatellite instability (MSI).

IPC Classes  ?

• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Abstract

The present invention describes a secondary tube tray for use in an automated processing system, the tray comprising a base module and a secondary tube insert. Furthermore, the present invention describes a secondary tube handling module of an automated processing system for automatically processing biological sample, and a method of handling secondary tubes for use in automatically processing biological sample in an automated processing system.

IPC Classes  ?

• B01L 9/06 - Test-tube stands; Test-tube holders
• B65C 9/02 - Devices for moving articles, e.g. containers, past labelling station
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

A sealing device configured to create a frangible seal in a sample processing device is described. Methods of using the device to create one or more frangible seals in a sample processing device are also described.

IPC Classes  ?

• B29C 65/18 - Joining of preformed parts; Apparatus therefor by heating, with or without pressure using heated tool
• B01L 3/14 - Test tubes
• C12M 1/24 - Apparatus for enzymology or microbiology tube or bottle type

Abstract

Methods and systems are provided for packaging reporter nucleic acid molecules into non- replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non- replicative transduction particles as reporter molecules.

IPC Classes  ?

• C12Q 1/6897 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
• C12Q 1/14 - Streptococcus; Staphylococcus
• C12Q 1/18 - Testing for antimicrobial activity of a material
• C12N 7/00 - Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

Abstract

Provided herein are methods and compositions for isolating and detecting nucleic acids from platelet-enriched plasma.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

IPC Classes  ?

• C07H 19/10 - Pyrimidine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C07H 19/20 - Purine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

The disclosure provides improved magnetic glass particles for use in nucleic acid capture, enrichment, analysis, and/or purification. Various modifications to the disclosed compositions and methods of using the same, devices, and kits are described.

IPC Classes  ?

• H01F 1/00 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C01G 49/00 - Compounds of iron

Abstract

The present invention describes a base module (100) and a tray insert (200, 300, 400) of a multipurpose tray (100) for an automated processing system, such as an analytical, pre-analytical or post- analytical processing system, as well as to a multipurpose tray comprising such base module and such tray insert, wherein the tray insert can be particularly used for holding a plurality of reagent or sample tubes to be processed in the automated processing system. The present invention further relates to a method of simplified loading/unloading of such a multipurpose tray into/from the automated processing system.

IPC Classes  ?

• B01L 9/06 - Test-tube stands; Test-tube holders
• G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
• B65D 77/04 - Articles or materials enclosed in two or more containers disposed one within another
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• B65D 25/10 - Devices to locate articles in containers
• A61J 1/16 - Holders for containers

Abstract

The present invention describes a laboratory system for automatically processing at least one sample container containing a biological sample, the laboratory system comprising a housing;laboratory instrument units for executing processing steps on the biological sample;an input station configured to receive the sample container; a transport means for transport of the sample container from the input station to the laboratory instrument units, and further to an output station;a control unit for determining whether the sample and/or the container is in a condition to be processed by the laboratory instrument units; an input-output station providing an interface between the inside and the outside of the housing; and a workbench on the outside of the housing in front of the input-output station for an operator to be in the position to manipulate the sample and/or the container. Furthermore, the use of such a laboratory system as well as a method for processing a biological sample in a sample container by means of such a laboratory system is described.

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
• G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system

Abstract

Modified or mutant bacterial luciferases having improved activity, as compared to wild type or unmodified bacterial luciferases, are described. The modified or mutant bacterial luciferases display increased light production and/or slower signal decay. Employing these modified or mutant bacterial luciferases improve a luminescence reporter system assay by decreasing the detection sensitivity, resulting in improved bioreporter/reporter assays. The invention further provides methods for using the modified or mutant bacterial luciferases, reporter assays using the modified or mutant bacterial luciferases, and kits and articles of manufacture.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12Q 1/66 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

Abstract

The present disclosure relates to a method for isolating a biological target material from a liquid sample in a multiwell plate using magnetic particles, wherein high efficiency and low elution volumes arc achieved by specific movements of the multiwell plate and a magnetic separation plate in relation to each other. Also disclosed is a pre-analytical system suitable for carrying out the method.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• B01L 9/00 - Supporting devices; Holding devices
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor

Abstract

Methods for the rapid detection of the presence or absence of Trichomonas vaginalis (TV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the TV beta tubulin gene, along with kits are provided that are designed for the detection of TV.

IPC Classes  ?

• C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Abstract

Provided herein are methods and compositions for performing multiplex RT-PCR to amplify short nucleic acids.

IPC Classes  ?

• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
• C12Q 1/6809 - Methods for determination or identification of nucleic acids involving differential detection

Abstract

BabesiaBabesia BabesiaBabesiaB microtiBdivergensBduncaniBBabesiaBabesia.

IPC Classes  ?

• C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Abstract

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises nucleoside polyphosphates having four or more phosphates. The present invention also provides for methods for using the nucleoside polyphosphates having four or more phosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

IPC Classes  ?

• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

Provided herein are methods and compositions for multiplex detection of a large number of actionable gene fusions with very high sensitivity and specificity. The present methods and compositions can detect ALK, RET, and ROS1 gene fusions, optionally in combination with other mutations and fusions.

IPC Classes  ?

• C12Q 1/6851 - Quantitative amplification
• C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Abstract

Catalysts act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting catalysts to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.

IPC Classes  ?

• G01N 1/30 - Staining; Impregnating

Abstract

A sample processing tubule is provided including, from a proximate to a distal end, an opening through which a sample is introducible, at least three segments, and an extraction port operatively connected to a distal segment of the at least three segments. The extraction port enables extraction of a reaction mixture in the distal segment of the tubule without piercing the tubule or one or more seals separating each of the segments in the tubule.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

A sample processing tubule is provided including, from a proximate to a distal end, an opening through which a sample is introducible, at least three segments, and an reagent introduction port operatively connected to a distal segment of the at least three segments. The reagent introduction port enables the addition of a reagent in the distal segment of the tubule, enabling the user to create a customizable assay tubule.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

Package assemblies for storing diagnostic cartridges or storing wet/dry reagents are described herein. In some embodiments, an assembly includes a tray member defining a first volume and a second volume, and a cover member coupled to the tray member covering the first volume and the second volume. The tray member includes a central portion that separates the first volume from the second volume. The first volume is configured to receive a desiccant package and a sample container containing a first reagent. The first reagent has a solid form. The second volume is configured to receive a reagent module containing a second reagent. The second reagent has a liquid form. The cover member and the central portion of the tray member are configured to isolate the first volume from the second volume.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers

Abstract

The disclosure is a single-tube multiplex assay, capable of simultaneously detecting multiple nucleic acid targets, using multiple hybridization primers and probes, labeled with the same fluorescent reporter label, but each having a distinct annealing temperature. The assay can be further multiplexed with the use of multiple sets of hybridization primers and probes, each set labeled with a separate fluorescent reporter label.

IPC Classes  ?

• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside polyphosphates. The present invention also provides for methods for using the modified nucleoside polyphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

IPC Classes  ?

• C07H 19/00 - Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro derivatives thereof
• C12Q 1/6844 - Nucleic acid amplification reactions

Abstract

Accurate measurements of the presence or absence of a target cell in a sample are provided. For example, the sample can be mixed with a plurality of transduction particles capable of binding to the target cells, the transduction particles being engineered to include a nucleic acid molecule formulated to cause the target cells to produce a plurality of detectable reporter molecules once the particles bind to and deliver the nucleic acid molecules into the one or more target cells. A set of signal data points are received that are associated with a quantity of reporter molecules and the signal data points are analyzed to accurately detect target cells in the sample. A curve is generated from the data gathered and analysed for peaks, which are mathematically transformed into positive signal data points using Area ratio, relative variation of the relative light units (RLU) and a linear threshold. Systems and methods are disclosed.

IPC Classes  ?

• C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
• C12Q 1/18 - Testing for antimicrobial activity of a material