Disclosed herein include novel blood-brain barrier (BBB)-crossing receptors on the BBB interface, targeting peptides and derivatives thereof capable of binding to the novel receptors, and related methods of using the receptors to increase the permeability of the BBB and to deliver an agent to a nervous system (e.g., CNS). In some embodiments, the BBB-crossing receptor is carbonic anhydrase IV. Disclosed herein also include recombinant adeno-associated viruses (rAAVs) with increased specificity and transduction efficiency across the BBB and related compositions and methods of treating various diseases and conditions.
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
C07K 16/40 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against enzymes
An optica I -th in -film structure comprises a low-index optical thin film consisting essentially of co-deposited Barium Fluoride and a secondary fluoride compound, and a high-index optical thin film.
G02B 1/02 - Optical elements characterised by the material of which they are made; Optical coatings for optical elements made of crystals, e.g. rock-salt, semiconductors
G01N 21/35 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
Disclosed herein include methods, compositions, and kits suitable for use in generating barcoded detection particles. Each barcoded detection particle can comprise a particle associated with an antigen-binding protein and a plurality of barcoding oligonucleotides. The plurality of barcoding oligonucleotides can comprise a first ligand. The particle can comprise a second ligand. The plurality of barcoding oligonucleotides can be associated with the particle via a multivalent binding agent comprising two or more binding moieties capable of binding the first ligand and/or the second ligand. There are provided, in some embodiments, methods for detecting interactions between nucleic acid molecules and proteins of interest. Methods for detecting interactions between ribonucleic acid molecules and RNA-binding proteins (RBPs) are also provided herein.
C08L 23/18 - Homopolymers or copolymers of hydrocarbons having four or more carbon atoms
C08L 101/00 - Compositions of unspecified macromolecular compounds
C08F 255/08 - Macromolecular compounds obtained by polymerising monomers on to polymers of hydrocarbons as defined in group on to polymers of olefins having four or more carbon atoms
A61B 17/00 - Surgical instruments, devices or methods, e.g. tourniquets
5.
HIGH THROUGHPUT MICROCRYSTAL SOAKING FOR STRUCTURAL ANALYSIS OF PROTEIN-LIGAND INTERACTIONS HIGH THROUGHPUT MICROCRYSTAL SOAKING FOR STRUCTURAL ANALYSIS OF PROTEIN-LIGAND INTERACTIONS
B01J 20/28 - Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
B01D 53/02 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography
7.
SUPPRESSION OF NON-SPECIFIC SIGNALS BY EXONUCLEASES IN FISH EXPERIMENT
Disclosed herein is a composition for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples. Also disclosed herein, is a kit for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples. Also disclosed herein, is a method for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples.
in vitroin vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.
Systems and methods for a self-powered wireless wearable sensor system include a photovoltaic (PV) panel array, used as a power source for a wearable sensor. The PV panel array may be attached to an area of the human body exposed to a light source. Exposure to a light source may generate an electric field and power a wearable device sufficiently to support data transmission and continuous monitoring. An integrated self-powered wireless wearable sensor system may include a microfluidic sweat sensor patch that may be connected to lower-power wireless sensor circuitry for regulating power efficiently and may be powered by the PV panel array.
B01D 53/02 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography
B01D 46/00 - Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
11.
METHOD OF MAPPING SPATIAL DISTRIBUTIONS OF CELLULAR COMPONENTS
This disclosure herein sets forth embodiments for a method for mapping the spatial distribution of one or more cellular components or cellular interactions by using barcodes to identify a cell or cellular component in the one or more cells, localizing, detecting, and mapping the spatial distribution of the one or more cellular components. The disclosure herein sets forth method to allow the mapping of spatial distributions of cellular components both intracellularly or intercellularly.
Disclosed herein include methods, compositions, and kits suitable for use in sorting a population of cells. In some embodiments, the method comprises flowing a fluid sample comprising a population of cells through a microfluidic channel. The population of cells can be configured to express gas vesicles (GVs) in a context-dependent manner. The expression of GVs within a cell can increase the compressibility (β) and reduce the density (ρ) of said cell, thereby modulating the acoustic contrast (Φ) of said cell relative to the fluid in the microfluidic channel. The method can comprise applying ultrasound to the microfluidic channel. Applying ultrasound can generate acoustic standing wave(s) in the microfluidic channel, thereby positioning pressure antinode(s) in the microfluidic channel.
Disclosed herein include antibodies or fragments thereof having specificity to a sarbecovirus spike protein receptor binding domain. Also provided are compositions, methods, and kits for isolating and using said antibodies or fragments thereof for preventing or treating, for example a coronavirus infection.
Systems and methods for an electronic skin based robotic system including a robotic interface and a human subject are provided. An e-skin may be applied to the robotic interface. The e-skin applied to the robotic interface may include a plurality of physicochemical sensors. An e-skin may also be applied to the human subject. The e-skin may include electrodes for sensing muscular contractions associated with hand and arm movements as well as electrodes for stimulation. Machine learning techniques may enable decoding of signals to control the robotic hand and arm. The robotic hand and arm may be controlled to approach unknown compounds that may be hazardous. The sensors making up the physicochemical sensors on the e-skin on the robotic hand and arm may include tactile, pressure, temperature, and chemical sensors, as well as other useful sensors. These sensors may enable detection of explosives, organophosphates, pathogenic proteins, and other hazardous compounds.
G01L 1/20 - Measuring force or stress, in general by making use of electrokinetic cells, i.e. liquid-containing cells wherein an electrical potential is produced or varied upon the application of stress
G01L 5/22 - Apparatus for, or methods of, measuring force, work, mechanical power, or torque, specially adapted for specific purposes for measuring the force applied to control members, e.g. control members of vehicles, triggers
15.
NON-INVASIVE METHOD AND DEVICE FOR CONTINUOUS SWEAT INDUCTION AND COLLECTION
Systems and methods for a microfluidic biosensor patch and health monitoring system may include an iontophoresis module, a multi-inlet microfluidic sweat collection and sampling module, and a molecularly imprinted polymer (MIP) organic compound sensor module. An iontophoresis module may provide for stimulation of a biofluid sample. A biofluid may be a sweat sample. Stimulation may be achieved via electrostimulation and/or application of a stimulating agent. A microfluidic sweat collection and sample module may include several adhesive layers with carefully designed inlets, channels, a reservoir, and an outlet for the efficient collection and sampling of biofluid. A MIP sensor module may quickly and accurately identify concentrations of key metabolites present in a biofluid sample which may indicate certain health conditions.
A61B 5/145 - Measuring characteristics of blood in vivo, e.g. gas concentration, pH-value
G16H 50/80 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for detecting, monitoring or modelling epidemics or pandemics, e.g. flu
G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
G16H 10/20 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for electronic clinical trials or questionnaires
Systems and methods for a smart bandage for monitoring and treating wounds are provided herein. The smart bandage may be a smart, wearable, flexible multi-layer substrate that may include a system that can monitor wound characteristics, perform autonomous bioanalysis of wound characteristics to determine wound treatment plans in a non-invasive method, perform drug delivery or antimicrobial agent release to treat and prevent infections, and promote healing by electrical stimulation. The smart bandage may be equipped with a wireless network that can communicate with users, such as patients and medical providers, directly about a patient's wound condition and provide for on-demand wound treatment.
Described herein are compositions and kits comprising recombinant adeno-associated viruses (rAAVs) with increased transduction enrichment in the heart and/or skeletal muscle and, in some cases, reduced transduction in the liver. The rAAV compositions described herein encapsidate a transgene, such as a therapeutic nucleic acid. Gene therapy using the rAAVs is described. Also described are methods of treating diseases and conditions of the heart and/or skeletal muscle.
A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermen
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
C07K 14/00 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
18.
SYSTEM FOR OPTICALLY ANALYZING A TEST SAMPLE AND METHOD THEREFOR
The present disclosure is directed toward measurement systems capable of optical analysis of a test sample. Embodiments in accordance with the present disclosure include a sample holder having a plurality of projections that extend from a planar surface, where the projections and planar surface collectively define an open sample-collection surface that enables an interrogation signal direct access to the test sample. The projections can be dimensioned and arranged to collectively define a geometric anti-reflection surface that is substantially non-reflective for the interrogation signal even at large angles of incidence. In some embodiments, the sample holder is configured as a reflective element that enables multiple passes of the interrogation signal through the test sample. In some embodiments, the sample holder is configured as a transmissive element. In some embodiments, the projections themselves are reflective.
G01N 21/3577 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
G01N 21/01 - Arrangements or apparatus for facilitating the optical investigation
G01N 21/35 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
The present disclosure provides engineered enzymes and methods for preparing tyrosine and tyrosine analogs from a donor amino acid and phenols in a single step. Also disclosed are methods to engineer enzymes having such 'tyrosine synthase' activity, starting from the beta-subunit of a tryptophan synthase (TrpB).
GOTTFRIED WILHELM LEIBNIZ UNIVERSITÄT HANNOVER (Germany)
HELMHOLTZ ZENTRUM MÜNCHEN - DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) (Germany)
Inventor
Plettenburg, Oliver
Ahlbrecht, Christin
Adalian, Dvin
Scherer, Axel
Madero, Xiomara Linnette
Chen, Samson
Jilani, Muhammad Musab
Abstract
Methods and systems are described for fabricating thin hydrogel layers on biosensors by a drop-spin method, which includes placing a drop of the hydrogel on the electrode, spinning the wafer at high speed in a vacuum, and heating the wafer to cure. One and multilayer sensors can be fabricated in this way, by adding layers of hydrogel or metal.
Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic macrophages through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow macrophages to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Genetic circuits capable of inducing expression of a payload upon thermal stimulation are provided. There are provided, in some embodiments, heat-inducible macrophages and methods of using are provided.
C07K 14/47 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from humans from vertebrates from mammals
A61K 35/15 - Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
Disclosed herein include methods, compositions, and kits suitable for use in calcium imaging. There are provided, in some embodiments, Ca2+-sensing GvpC proteins. Disclosed herein include Ca2+-sensing gas vesicles (GVs) comprising Ca2+-sensing GvpC proteins. In some embodiments, the Ca2+-sensing GvpC protein is capable of undergoing a first allosteric conformational change upon the Ca2+-binding domain binding Ca2+that causes the Ca2+-sensing GV to change from a GV stiff state to a GV soft state. One or more of the mechanical, acoustic, surface, and magnetic properties of a Ca2+-sensing GV can differ between the GV soft state and the GV stiff state.
Disclosed herein include a photoactivatable vibrational probe, when photoactivated, capable of forming a Raman probe for detection by Raman scattering. In some embodiments, the photoactivatable vibrational probe has a structure of Formula I. Disclosed herein also includes methods of using the photoactivatable vibrational probe for live-cell multiplexed imaging and tracking.
Disclosed herein include methods, compositions, and kits suitable for use in imaging of in situ gene expression. There are provided, in some embodiments, viral vector compositions. Disclosed herein includes a single viral vector comprising one or more gas vesicle assembly (GV A) gene(s) encoding one or more GV A protein(s), and one or more gas vesicle structural (GVS) gene(s) encoding one or more GVS protein(s). The one or more GV A protein(s) and the one or more GVS protein(s) can be capable of forming gas vesicles (GVs) upon expression in a cell.
Systems and methods for identifying the components of a long-chain molecule by making electrical measurements from fabricated nanoscale electrodes as the molecule moves down a narrow microfluidic channel. The channel can be along the surface of a chip, through a chip, or both.
Disclosed herein include compositions and kits comprising recombinant adeno-associated viruses (rAAVs) with tropisms to the central nervous system, the peripheral nervous system, and/or the lung, with increased specificity and transduction efficiency. Also described include methods of treating various diseases and conditions using the rAAVs.
C07K 14/005 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 11/00 - Drugs for disorders of the respiratory system
A61P 25/00 - Drugs for disorders of the nervous system
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
28.
SYSTEMS AND METHODS FOR GAS-LIQUID CONTACTORS FOR RAPID CARBON CAPTURE
Disclosed herein include methods, compositions, and kits suitable for use in the delivery of polyribonucleotides and circuits. There are provided, in some embodiments, RNA exporter proteins comprising an RNA-binding domain, a membrane-binding domain, and an interaction domain capable of nucleating self-assembly. Disclosed herein include polynucleotides encoding cargo RNA molecule(s). In some embodiments, a plurality of RNA exporter proteins are capable of self-assembling into lipid-enveloped nanoparticles (LNs) secreted from a sender cell in which the RNA exporter proteins are expressed, thereby generating a population of LNs comprising a fusogen and exported cargo RNA molecule(s).
Disclosed herein include methods, compositions, and kits suitable for use in the measurement of the states of living cells across time. There are provided, in some embodiments, RNA exporter proteins comprising an RNA-binding domain, a membrane-binding domain, and an interaction domain capable of nucleating self-assembly. Disclosed herein include polynucleotides encoding reporter RNA molecule(s). In some embodiments, a plurality of RNA exporter proteins are capable of self-assembling into lipid-enveloped nanoparticles (LNs) secreted from a reporter cell in which the RNA exporter proteins are expressed, thereby generating a population of LNs comprising exported reporter RNA molecule(s).
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
Systems and methods are provided for a non-invasive high resolution pressure pulse waveform measurement system. The system may include a blood pressure cuff, an air pump to inflate the blood pressure cuff to specific pressure levels, high resolution pressure sensors configured to perform high sensitivity signal acquisition at a specified pressure level, high range pressure sensors configured to measure an absolute reference for the signal and to calibrate the signal, pneumatic tubing connecting the air pump and sensors with the cuff, and a hydrodynamic filter configured as an input to a reference port of the high resolution pressure sensor. The hydrodynamic filter may be configured to transmit only mean pressure by attenuating a selected frequency range of the signal.
A61B 5/02 - Measuring pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography; Heart catheters for measuring blood pressure
Systems and methods disclosed herein relate to the detection of irregular particles in a blood flow based on a determined relative speed of a particle suspended in a blood flow and/or other properties of a particle suspended in a blood flow including a particle's relative position within a blood vessel and a particles tendency to cluster with other particles suspended in a blood flow. Based on a determined relative speed and/or other relevant factors, the properties of irregular particles may also be measured, including the size, shape, and frequency of irregular particles in a blood flow. Machine learning techniques may be employed to determine patterns for the behavior of irregular particles suspended in a blood flow. These patterns may correspond to particular health risks and conditions.
Disclosed herein include adeno-associated virus (AAV) acoustic targeting peptides. An AAV comprising the AAV acoustic targeting peptide can exhibit increased transduction at site(s) of focused ultrasound blood-brain barrier opening (FUS-BBBO), increased neuronal tropism, and diminished transduction of peripheral organs. Disclosed herein include recombinant adeno-associated virus (rAAV) comprising an AAV acoustic targeting peptide disclosed herein. Also provided herein include methods of delivering an agent to one or more target brain region(s) of a subject.
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61K 9/00 - Medicinal preparations characterised by special physical form
A61P 25/00 - Drugs for disorders of the nervous system
34.
STOICHIOMETRIC EXPRESSION OF MESSENGER POLYCISTRONS
Disclosed herein include methods, compositions, and kits enabling expression of multiple proteins from a single mRNA with a predetermined stoichiometry. There are provided, in some embodiments, nucleic acid compositions comprising a promoter operably linked to a polynucleotide comprising a first nucleic acid unit encoding first unit payload protein(s) and a second nucleic acid unit encoding second unit payload protein(s). The first nucleic acid unit and the second nucleic acid unit can each comprise a 3' engineered translation initiation site (eTIS) comprising a three-nucleotide tunable element immediately upstream of a start codon. The eTIS of each of the first nucleic acid unit and the second nucleic acid unit can be configured to achieve a predetermined stoichiometry of the first unit payload protein(s) and the second unit payload protein(s) in a cell or cell-like environment.
C12N 15/63 - Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/67 - General methods for enhancing the expression
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
C07K 14/00 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
35.
STAIN-FREE DETECTION OF EMBRYO POLARIZATION USING DEEP LEARNING
Disclosed herein include systems, devices, and methods for detecting embryo polarization from a 2D image generated from a 3D image of an embryo that is not fluorescently labeled using a convolutional neural network (CNN), e.g., deep CNN.
Provided herein are methods and compositions for inhibiting p97, for the treatment of a motor neuron disease in a subject, or a symptom thereof. Upon treatment, the motor neuron disease, or a symptom thereof is reduced in the subject.
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
A61K 31/4439 - Non-condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
A61K 31/519 - Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
A self-deployable antenna and/or antenna array that is made up of one or more antenna elements. Each of the antenna elements has a structural base that supports portions of the antenna and can be positioned between a stored configuration for compaction and a deployed configuration for transmitting. The antenna elements and structural base can be part of a base substrate that provides a base support for the antenna and/or antenna array to be compacted and deployed.
H01Q 1/38 - Structural form of radiating elements, e.g. cone, spiral, umbrella formed by a conductive layer on an insulating support
H01Q 9/28 - Conical, cylindrical, cage, strip, gauze or like elements having an extended radiating surface; Elements comprising two conical surfaces having collinear axes and adjacent apices and fed by two-conductor transmission lines
H01Q 19/30 - Combinations of primary active antenna elements and units with secondary devices, e.g. with quasi-optical devices, for giving the antenna a desired directional characteristic using a secondary device in the form of two or more substantially straight conductive elements the primary active element being centre-fed and substantially straight, e.g. Yagi antenna
Disclosed herein include methods, compositions, and kits suitable for use in vaccination. There are provided, in some embodiments, nucleic acid compositions (e.g., mRNA vaccine, DNA vaccine) comprising a polynucleotide encoding a fusion protein. The fusion protein can comprise an antigenic polypeptide (AP) and an endosomal sorting complex required for transport (ESCRT)-recruiting domain (ERD). A plurality of fusion proteins can be capable of self-assembling into an enveloped nanoparticle (ENP) secreted from a cell in which the fusion proteins are expressed. There are provided, in some embodiments, populations of ENPs.
Disclosed herein are methods for generating an ratiometric symbol for sequential hybridization barcoding for multiplexed Fluorescence In Situ Hybridization (FISH). Also, the disclosure sets forth methods, in addition to using the same, and other solutions to problems in the relevant field.
A structure having a tunable modulus of bending (flexibility) composed of interlocking geometric particles arranged such that an external pressure/force causes them to jam together, increasing the stiffness of the overall structure. Methods for creating the external pressure can include the use of an envelope around the structure that can be evacuated of air.
B29C 43/10 - Isostatic pressing, i.e. using non-rigid pressure-exerting members against rigid parts or dies
B29C 64/153 - Processes of additive manufacturing using only solid materials using layers of powder being selectively joined, e.g. by selective laser sintering or melting
Disclosed herein is a composition for linked amplification tethered with exponential radiance for signal amplification. Also disclosed herein, is a kit for linked amplification tethered with exponential radiance for signal amplification. Also disclosed herein, is a method linked amplification tethered with exponential radiance for signal amplification.
Disclosed herein are methods for generating a massive number of chemically ligateable probes for multiplexed Fluorescence In Situ Hybridization (FISH) using a hybrid- primer. Also, the disclosure sets forth methods, in addition to using the same, and other solutions to problems in the relevant field.
Systems and methods for a microfluidic biosensor patch and health monitoring system may include an iontophoresis module, a multi-inlet microfluidic sweat collection and sampling module, and a molecularly imprinted polymer (MIP) organic compound sensor module. An iontophoresis module may provide for stimulation of a biofluid sample. A biofluid may be a sweat sample. Stimulation may be achieved via electrostimulation and/or application of hydrogel. A microfluidic sweat collection and sample module may include several adhesive layers with carefully designed inlets, channels, a reservoir, and an outlet for the efficiently collection and sampling of biofluid. A MIP sensor module may quickly and accurately identify concentrations of key metabolites present in a biofluid sample which may indicate certain health conditions.
Disclosed herein include compositions and kits comprising recombinant adeno- associated viruses (rAAVs) with tropisms to the central nervous system with increased specificity and transduction efficiency, including endothelial cells of the neurovascular unit. Also described include methods of treating various diseases and conditions using the rAAVs.
C07K 7/06 - Linear peptides containing only normal peptide links having 5 to 11 amino acids
C07K 14/005 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 25/00 - Drugs for disorders of the nervous system
46.
SINGLE CHAIN TRIMER MHC CLASS I NUCLEIC ACIDS AND PROTEINS AND METHODS OF USE
Peptide-major histocompatibility (MHC) Class I nucleic acids and proteins are provided. Methods of their use, for example in methods of identifying antigen-specific T cells and adoptive cell therapy, are also provided.
A coherent imaging system including a transmitter and a receiver. The transmitter includes a coherent source and a power splitter for splitting the electromagnetic radiation into a reference and a signal beam. The receiver includes an image forming device and an array of pixels. Each of the pixels include means for collecting at least a portion of the signal beam imaged on the pixel by an image forming device, as a collected signal; means for splitting the collected signal into a plurality of collected signals each having different phase shifts; means for mixing each of the collected signals with the reference beam so as to form a plurality of mixed signals; and means for detecting the mixed signals and outputting a plurality of output electrical signals in response to the mixed signals.
G01R 33/54 - Signal processing systems, e.g. using pulse sequences
G01R 33/34 - Constructional details, e.g. resonators
G01R 33/36 - Electrical details, e.g. matching or coupling of the coil to the receiver
G01R 25/04 - Arrangements for measuring phase angle between a voltage and a current or between voltages or currents involving adjustment of a phase shifter to produce a predetermined phase difference, e.g. zero difference
48.
TILED POROUS ARCHITECTED COMPOSITIONS, METHODS OF THEIR MAKING
Provided herein are compositions having a porous, architected three-dimensional geometry that are tiled to achieve increased surface area and/or volume. The tiled compositions include a plurality of tiles having seams where one of the plurality of tiles contacts an adjacent tile. Also provided herein are methods and systems for making the compositions.
B29C 64/124 - Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
B29C 64/236 - Driving means for motion in a direction within the plane of a layer
B29C 64/282 - Arrangements for irradiation using multiple radiation means, e.g. micromirrors or multiple light-emitting diodes [LED] of the same type, e.g. using different energy levels
The present disclosure provides methods for analyzing genomic structures by diffraction limited locus imaging and nucleic acid block coding. The methods allow efficient and scalable imaging, which can be applied to multiplexed RNA/DNA fluorescence in situ hybridization (FISH).
C07D 417/14 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group containing three or more hetero rings
A61K 31/437 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a five-membered ring having nitrogen as a ring hetero atom, e.g. indolizine, beta-carboline
A61K 31/4365 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system having sulfur as a ring hetero atom, e.g. ticlopidine
A61K 31/428 - Thiazoles condensed with carbocyclic rings
A61K 31/429 - Thiazoles condensed with heterocyclic ring systems
A61K 31/506 - Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
A61K 31/496 - Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene
A61P 35/02 - Antineoplastic agents specific for leukemia
C07D 417/14 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group containing three or more hetero rings
A61K 31/437 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a five-membered ring having nitrogen as a ring hetero atom, e.g. indolizine, beta-carboline
A61K 31/4365 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system having sulfur as a ring hetero atom, e.g. ticlopidine
A61K 31/428 - Thiazoles condensed with carbocyclic rings
A61K 31/429 - Thiazoles condensed with heterocyclic ring systems
A61K 31/506 - Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
A61K 31/496 - Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene
Systems and methods for measuring flow velocity of a fluid mixture in a lateral section of an oil/gas/water well with a dual beam laser doppler velocimetry (LVD) based flow sensor are presented. According to one aspect, the flow velocity is measured by tracking movement of particles and/or features in the fluid mixture while traversing an interference pattern generated by the intersection of two separate coherent beams that are perpendicular to a direction of the flow. Flow velocity is derived based on a time it takes the particles to traverse consecutive fringes of the interference pattern as indicated by intensity peaks detected at the photodetector. The LDV-based flow sensor may be rotatable to measure flow velocities at different angular positions of a pipe in a lateral section of an oil well, rotation provided by rotation of an element of a mobile vessel to which the flow sensor is rigidly coupled.
G01P 5/26 - Measuring speed of fluids, e.g. of air stream; Measuring speed of bodies relative to fluids, e.g. of ship, of aircraft by measuring the direct influence of the streaming fluid on the properties of a detecting optical wave
G01P 5/20 - Measuring speed of fluids, e.g. of air stream; Measuring speed of bodies relative to fluids, e.g. of ship, of aircraft by measuring the time taken by the fluid to traverse a fixed distance using particles entrained by a fluid stream
G01F 1/7086 - Measuring the time taken to traverse a fixed distance using optical detecting arrangements
G01F 1/712 - Measuring the time taken to traverse a fixed distance using auto-correlation or cross-correlation detection means
G01F 1/74 - Devices for measuring flow of a fluid or flow of a fluent solid material in suspension in another fluid
53.
SYSTEM FOR MEASURING MULTIPHASE FLOW IN DOWNHOLE CONDITIONS AND FLOW REGIMES
Systems and methods for measuring multiphase flow of a fluid mixture in a downhole pipe of an oil/gas/water well are presented. According to one aspect, time-series measurement of the flow velocity and composition at a plurality of discrete azimuths of the pipe are measured. Measured time-correlated velocity and composition data are used to identify fluid components present in the pipe and estimate cross-sectional area and velocity of each of the fluid components. According to another aspect, pressure and temperature at the downhole pipe are measured, and used to calculate the mass density of each fluid component. For each of the fluid components, the cross-sectional area, velocity, and mass density are used to generate a corresponding mass flow rate. An algorithm with a set of parameters tuned to specific flow regimes is used to map the sensed data from the time-series measurements into the mass flow rate of each fluid component.
G01F 1/7086 - Measuring the time taken to traverse a fixed distance using optical detecting arrangements
G01F 1/712 - Measuring the time taken to traverse a fixed distance using auto-correlation or cross-correlation detection means
G01F 1/74 - Devices for measuring flow of a fluid or flow of a fluent solid material in suspension in another fluid
G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
54.
ACOUSTIC REMOTE CONTROL OF MICROBIAL IMMUNOTHERAPY
Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of probiotic cells. There are provided, in some embodiments, thermal bioswitches that allow probiotic cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Genetic circuits capable of inducing expression of a payload upon thermal stimulation are provided. Thermally actuated probiotic cells and methods of use for the treatment of diseases or disorders are also provided.
Systems and methods for driving and steering of a robot, or mobile vessel, for operation in a downhole pipe of an oil/gas/water well are presented. According to one aspect, the mobile vessel includes a plurality of wheels arranged outwardly from the mobile vessel so that each wheel may contact the inner wall of the casing. According to another aspect, the plurality of wheels may include respective wheel-centerlines that intersect the centerline of the casing that is at an offset from a centerline of the mobile vessel. The plurality of wheels includes at least two drive wheels and at least one passive wheel. According to a further aspect, the drive wheels are configured to rotate about their respective wheel-centerlines to steer the mobile vessel during traversal of the casing. In one case, the mobile vessel includes two drive and steering wheels and one passive wheel.
Interplanetary networks for space internet and space positioning are presented. The described networks deploy spacecraft swarms along the solar system to form a science and communications platform. The disclosed network nodes are placed around planetary Lagrange points. Creation of optical synthetic apertures using smallsat swarms for inter- and intranet communications is further described. Exemplary subnetworks such as the cislunar network are also presented.
G01S 19/02 - Satellite radio beacon positioning systems transmitting time-stamped messages, e.g. GPS [Global Positioning System], GLONASS [Global Orbiting Navigation Satellite System] or GALILEO - Details of the space or ground control segments
H04B 10/118 - Arrangements specific to free-space transmission, i.e. transmission through air or vacuum specially adapted for satellite communication
57.
SYSTEMS AND METHODS FOR EFFICIENT CRUISE AND HOVER IN VTOL
A system of a multi-rotor aircraft that capitalizes on the advantages of fixed wing elements combined with rotary wing structures. The fixed wing elements can help to generate lift once the aircraft is airborne and can thus reduce the need for larger lifting rotors which can allow for longer flight times and distances. Additionally, the systems disclosed herein take advantage of a partial in-wing configuration with a number of rotors to reduce the overall footprint of the vehicle while maintaining the flight efficiency that comes with combining features of fixed and rotary wing elements, and increasing operator safety by shrouding rotating parts. The unique configurations allow for a decoupling of the pitch, yaw and roll authority to reduce the complexity in control systems and improve the flight efficiency of the aircraft. Additional configurations implement the use of smaller thrust rotors that can be used to generate thrust as well as control yaw and thus counteract any remaining unbalanced torque.
SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE (USA)
CALIFORNIA INSTITUTE OF TECHNOLOGY (USA)
THE SALK INSTITUTE FOR BIOLOGICAL STUDIES (USA)
Inventor
Friedman, Rick A.
Boussaty, Ely
Hoelz, Andre
Olson, Steven
Shaw, Reuben
Abstract
The present disclosure provides compositions and methods for prevention or treatment of hearing loss. In some examples, a composition for preventing or treating hearing loss comprises at least one compound that activates AMPK in at least one hair cell.
A61K 31/5377 - 1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
A61K 31/437 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a five-membered ring having nitrogen as a ring hetero atom, e.g. indolizine, beta-carboline
A61K 31/4985 - Pyrazines or piperazines ortho- or peri-condensed with heterocyclic ring systems
59.
NEURAL-NETWORK-OPTIMIZED DEGREE-SPECIFIC WEIGHTS FOR LDPC MINSUM DECODING
Neural Normalized MinSum (N-NMS) decoding for improving frame error rate (FER) performance on linear block codes over conventional normalized MinSum (NMS). Dynamic multiplicative weights are assigned to each check-to-variable message in each iteration to efficiently provide training parameters of N-NMS that support N-NMS for longer block lengths. Embodiment are described for neural two-dimensional normalized MinSum (N-2D-NMS) decoders requiring fewer training parameters. The N-2D-NMS approaches for example use the same weight for edges with the same check and/or variable node degree. Simulation results indicate that that this LDPC decoding performs similarly to previous techniques while substantially reducing the amount of training necessary.
H03M 13/11 - Error detection or forward error correction by redundancy in data representation, i.e. code words containing more digits than the source words using block codes, i.e. a predetermined number of check bits joined to a predetermined number of information bits using multiple parity bits
H03M 13/00 - Coding, decoding or code conversion, for error detection or error correction; Coding theory basic assumptions; Coding bounds; Error probability evaluation methods; Channel models; Simulation or testing of codes
222-content feed streams, including air, and method of making and using the same. In some embodiments, the compositions comprise zinc-ion-doped zeolites having AEI, AFX, or CHA topologies.
Computational refocusing-assisted deep learning methods, apparatus, and systems are described. In certain pathology examples, a representative image is generated using a machine learning model trained with uniformly focused training images generated by a Fourier ptychographic digital refocusing procedure and abnormalities are automatedly identified and/or enumerated based on the representative image.
Method for detecting and barcoding the molecular changes occurring in two or more samples upon exposure to different stimuli. This disclosure herein sets for methods that allow a targeted transcriptome-imaging approach that records both gene-expression and spatial context within microscale assemblies at a single-cell and molecule resolution on biological samples. This disclosure herein sets for methods that allows the application to a variety of biological samples for the study of cellular processes, growth, and interactions between biological samples.
A61K 31/444 - Non-condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a six-membered ring with nitrogen as a ring hetero atom, e.g. amrinone
A61K 31/567 - Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. oestrane, oestradiol substituted in position 17 alpha, e.g. mestranol, norethandrolone
A61K 9/00 - Medicinal preparations characterised by special physical form
The present disclosure is directed to metal ion-containing zeolific compositions, preferably transition metal ion-containing, more preferably zinc ion containing zeolitic compositions, that are useful for reversibly scavenging water from humid gaseous feed streams, including air, and method of making and using the same. In some embodiments, the compositions comprise zinc-ion-doped zeolites have LTA, FAU, or EMT topologies.
Provided herein is an antibiotic susceptibility and related compositions, methods and systems based on nucleic acid detection based on detected intracellular and extracellular nucleic acid from a same sample, which allows determination of antibiotic susceptibility of microorganisms as well as the diagnosis and/or treatment of related infections in individuals.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/18 - Testing for antimicrobial activity of a material
Nucleic acids provide a versatile platform for engineering synthetic biology in medicine, science, agriculture, and energy. Degradation of nucleic acid molecules poses an engineering challenge. Nucleic acid protective elements (PELs) protect chemically synthesized or expressed nucleic acid molecules from degradation. PELs are derived from all or part of a viral xrRNA sequence and/or structural motif, or from rationally designed sequences and/or structural motifs, or they are engineered using directed evolution. PELs may also comprise a mixture of biologically derived, rationally designed sequence and/or structural motifs, and/or sequences and/or structural motifs engineered by directed evolution. PELs significantly enhance the performance of nucleic acids, protecting the regulatory and/or structural elements from degradation to increase regulatory dynamic range, fractional dynamic range, fold-change, and/or other performance metrics. PELs are used in applications including therapeutics, diagnostics, biological research tools, vaccines, crop protection, molecular manufacturing, sustainable energy production, and other areas involving nucleic acids.
Disclosed herein are allosteric conditional gRNA (cgRNA) mechanisms for both ON->OFF logic (conditional inactivation by an RNA trigger) and OFF->ON logic (conditional activation by an RNA trigger). Allosteric cgRNAs enable restriction of CRISPR/Cas function to a desired cell type, tissue, organ, or disease state. Allosteric cgRNAs provide a versatile platform for cell-selective and tissue-selective research tools, biotechnologies, diagnostics, and therapeutics.
A system and method for controlling a multi-rotor aircraft that implements the unconventional use of different sized rotors. The different sized rotors than the main rotors tend to generate an unbalanced torque and pitch on the aircraft that effectively decouples the pitch and yaw control from the main rotors. The atypical design tends to lend itself to improved control capabilities and simplified control systems. Additional configurations implement the use of smaller thrust rotors that can be used to generate thrust as well as control yaw and thus counteract any remaining unbalanced torque from the odd auxiliary rotor.
Relationships between alarms are modeled using a graph to identify root cause of a sequence of alarms (an alarm flood). The nodes of the graph represent different alarms, and the edges between the nodes are scored using pairwise analysis of when the alarms occurred. The graph is divided into multiple subgraphs representing alarm clusters. Root cause analysis of the sequence of alarms is performed by generating and simplifying directed graphs for events within the alarm clusters.
A network of high-resolution cameras for monitoring and controlling a drone within a specific operational environment such that the latency time for communication between the cameras and drone is less than that of human controlled drones. The drone can communication drone health data to the network of cameras where such information can be combined with visual image data of the drone to determine the appropriate flight path of the drone within the operational environment. The drone can then subsequently be controlled by the network of cameras by maintaining a constant visual image and flight control data of the drone as it operates within the environment.
A system and method for controlling a multi-rotor aircraft that implements a number of different lifting rotors that are configured to provide lift to the vehicle. Additionally, each of the lifting rotors have a corresponding yaw control rotor that can help provide yaw control as well as additional sideways movement control.
A composite, method of making the composite, and method of using the composite are disclosed. The composite comprises a macroporous scaffold comprising pores; and a polymer matrix positioned within the pores; wherein the polymer matrix comprises: a functional polymer particle; and a structural polymer. The method of using can comprise applications such as chromatography, catalysis, and sensing, among others.
The systems and methods for wastewater treatment comprise a porous substrate with photocatalytic material(s), and optionally electrocatalytic material(s), for generation of hydroxyl radicals that decompose contaminants in wastewater. The systems and methods intentionally facilitate the photocatalytic and/or electrocatalytic generation of oxygen bubbles, which facilitate an increase in concentration of hydroxyl radicals and shrink the liquid layer of wastewater at photocatalytic and/or electrocatalytic surfaces where hydroxyl radicals are generated in order to decrease quenching of hydroxyl radicals and simultaneously increase utilization or efficiency of the hydroxyl radicals for decomposition of contaminants. In embodiments, the systems and methods prolong hydroxyl radical lifetime and enhance water treatment performance for residential, commercial, municipal, medical, and/or industrial applications. In embodiments, oxygen bubbles generated on surfaces are compressed into pores or microchannels where they store generated hydroxyl radicals to prolong their lifetime in the gas phase and exchange hydroxyl radicals with the adjacent liquid layer.
Disclosed herein include engineered opioid biosensors, and related compositions, vectors, cells, and systems. Also disclosed include methods that provide opioid biosensors with sensitivity and selectivity suitable for continuous opioid monitoring as well as the use of the opioid biosensors for detecting one or more specific opioids. The opioid biosensors, which are capable of undergoing a detectable conformational change upon binding to an opioid, can each comprise a first periplasmic binding protein (PBP) domain and a second PBP domain connected to the first PBP domain, wherein at least one of the first PBP domain and the second PBP domain comprises one or more mutations.
Disclosed herein include circuits, compositions, nucleic acids, populations, systems, and methods enabling single circuits to generate multiple molecularly and functionally distinct states that are each stable across multiple cell division cycles. Synthetic circuits provided herein can stably exist in multiple distinct states characterized by differences in the concentrations and expression levels of its components. In the absence of changes to the external environment, each of these states can be stable. In some embodiments, transcription factors provided herein activate when dimerized, and show much weaker activity as monomers. In some embodiments, each transcription factor homodimer activates expression of its own gene. In some embodiments, transcription factors can form mixed heterodimers with one another that do not strongly activate any genes in the circuit. Different embodiments of the synthetic circuits provided herein can use different numbers of transcription factors to produce a growing number of stable states.
C12N 15/63 - Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/90 - Stable introduction of foreign DNA into chromosome
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A system and method for controlling a multi-rotor aircraft that implements the unconventional use of an odd number of rotors. The odd or auxiliary rotor is designed to be smaller in diameter than the remaining main rotors and accordingly generates a smaller unbalanced torque and pitch on the aircraft. Additional configurations implement the use of smaller thrust rotors that can be used to generate thrust as well as control yaw and thus counteract any remaining unbalanced torque from the odd auxiliary rotor.
The invention relates to compounds of Formula I, and pharmaceutically acceptable salts thereof, and methods of making and using the same. The compounds of the invention are effective in inhibiting KRAS protein with a G12C mutation and are suitable for use in methods of treating cancers mediated, in whole or in part, by KRAS G12C mutation.
C07D 403/14 - Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group containing three or more hetero rings
A61K 31/517 - Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with carbocyclic ring systems, e.g. quinazoline, perimidine
Disclosed herein is a membrane-based, in-gel loop-mediated isothermal amplification (LAMP) system, kit and method for detection of a target microorganism in a sample suspected of containing the microorganism. LAMP reagents, a lysing agent and a hydrogel are placed together on a filter membrane loaded with a pre-filtered sample. The hydrogel polymerizes over a short time to immobilize any target DNA/RNA particles on the membrane. The system may include a compact, portable device that integrates heat incubation and fluorescence illumination, and also a cloud-based smartphone image analysis application for quantitative results interpretation. If target DNA/RNA are present in the sample, fluorescent amplicons are produced as a result of LAMP reaction. The target microorganisms are detected by visually detecting the presence or absence of the amplicons. The method may be employed for rapid and inexpensive point-of-use (POU) absolute quantification of SARS-CoV-2 in environmental water or wastewater samples with high sensitivity.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
G02B 21/16 - Microscopes adapted for ultraviolet illumination
80.
MULTIVALENT CARRIERS AND RELATED VACCINE COMPOSITIONS
Disclosed herein include multivalent carriers comprising a plurality of heterologous coronavirus proteins antigens derived from different coronaviruses. The multivalent carriers herein described can elicit heterologous binding and neutralization properties against coronaviruses that differ from the coronaviruses from which the coronavirus antigens are derived to produce the multivalent carriers. Also provided herein include vaccine compositions comprising the multivalent carriers and related methods using the vaccine compositions in various therapeutic and prophylactic applications.
YEDA RESEARCH & DEVELOPMENT CO. LTD. AT THE WEIZMANN INSTITUTE OF SCIENCE (Israel)
Inventor
Newman, Dianne K.
Vandrisse, Chelsey M.
Lipsh-Sokolik, Rosalie
Khersonsky, Olga
Fleishman, Sarel J.
Abstract
Provided herein are engineered pyocyanine demethylases having replacements in in positions A53, I73, A87, T91, M99, A129 and K141 of pyocyanine demethylase PodA of SEQ ID NO: 1 or a derivative thereof and related phenazine degrading agents, compositions methods and systems, as well as a combined administration of one or more pyocyanine demethylases and antibiotics and/or antibiotics resulting in a synergic inhibition of viability of phenazine producing bacteria, and related phenazine degrading agents, compositions methods and systems.
B01D 53/04 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography with stationary adsorbents
B01D 53/22 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by diffusion
A system includes, in part, a multitude of RF receivers, a first optical modulator adapted to modulate an optical signal in accordance with a first data received by a first one of RF receivers to generate a first modulated signal, a second optical modulator adapted to modulate the optical signal in accordance with a second data received by a second RF receiver to generate a second modulated signal, and a first optical fiber adapted to carry the first and second modulated signals. The optical modulator may be a photodiode or a Mach-Zehnder modulator.
H04B 10/2519 - Arrangements specific to fibre transmission for the reduction or elimination of distortion or dispersion due to chromatic dispersion using Bragg gratings
85.
OPTICALLY ENABLED RF PHASED-ARRAYS FOR DATA TRANSMISSION
A system includes, in part, a first optical modulator adapted to modulate a first optical signal with a first data to generate a first modulated optical signal, a second optical modulator adapted to modulate a second optical signal with a first clock signal to generate a second modulated optical signal, an optical multiplexer adapted to multiplex the first and second optical signals to generate a multiplexed optical signal, and an optical fiber adapted to carry the multiplexed optical signal. The second optical signal has a second wavelength that is different from the first wavelength.
H03L 7/08 - Automatic control of frequency or phase; Synchronisation using a reference signal applied to a frequency- or phase-locked loop - Details of the phase-locked loop
G02B 6/42 - Coupling light guides with opto-electronic elements
G02B 6/28 - Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals
Some embodiments herein relate generally to compositions comprising microbial organisms and/or components thereof for improving a neurological disorder, or symptoms associated with a neurological disorder, such as Parkinson's disease, and methods of using the same.
A system and method for improving the flight control and efficiency of an aerial vehicle. Many embodiments are directed to a rotor-shroud assembly system where a plurality of rotor blades are connected to the internal side of a shroud and are set up to pivot through the use of a pitching mechanism. The entire assembly is configured to rotate when attached to a motor.
Disclosed herein include circuits, compositions, nucleic acids, populations, systems, and methods enabling cells to sense, control, and/or respond to their own population size. In some embodiments, an orthogonal communication channel allows specific communication between engineered cells. Also described herein, in some embodiments, is an evolutionarily robust 'paradoxical' regulatory circuit architecture in which orthogonal signals both stimulate and inhibit net cell growth at different signal concentrations. In some embodiments, engineered cells autonomously reach designed densities and/or activate therapeutic or safety programs at specific density thresholds. Methods of treatment are also provided in some embodiments.
C12N 15/66 - General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
C07K 14/415 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Systems and methods for epidemic/pandemic management by creating a network of user devices wirelessly connected to a central server or servers, the devices having location/proximity capability. The central server propagates crowdsourced information about individual risks of exposure and infectiousness across a dynamic contact network, where the risk assessments are determined by the server by data assimilation methods with corrections made by updating previous risk network models and re-evolving them.
G16H 50/80 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for detecting, monitoring or modelling epidemics or pandemics, e.g. flu
G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
90.
ADENO-ASSOCIATED VIRUS COMPOSITIONS HAVING PREFERRED EXPRESSION LEVELS
Described herein are compositions and kits comprising recombinant adeno- associated viruses (rAAVs) with tropisms showing increased viral transduction in the CNS. The rAAV compositions described herein encapsidate a transgene, such as a therapeutic nucleic acid. Gene therapy using the rAAVs is described. Also described are methods of treating CNS-related diseases and conditions.
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Disclosed herein is a phased antenna array which is configured to provide a signal through multiple antennas. The phased antenna array may include a plurality of sub¬ arrays. Each sub-array may include a complete and functional optically synchronized integrated circuit including an integrated photo diode. Advantageously, integrating the photo diode into the integrated circuit may reduce the size of the sub-array and decrease the length the high frequency timing signal is passed.
H01Q 3/34 - Arrangements for changing or varying the orientation or the shape of the directional pattern of the waves radiated from an antenna or antenna system varying the distribution of energy across a radiating aperture varying the phase by electrical means
H01Q 1/22 - Supports; Mounting means by structural association with other equipment or articles
H01Q 1/38 - Structural form of radiating elements, e.g. cone, spiral, umbrella formed by a conductive layer on an insulating support
92.
DPAGT1 INHIBITORS OF CAPURAMYCIN ANALOGUES AND THEIR ANTIMIGRATORY ACTIVITIES OF SOLID TUMORS
Provided herein are compounds and methods of using these compounds to treat disorders related to DPAGT1 function, including cancer and bacterial infections.
Systems and methods for measuring flow velocity of a fluid mixture in a lateral section of an oil/gas well are presented. The flow velocity is measured by tracking movement of particles and/or features in the fluid mixture via visible and/or infrared imaging sensors of a camera-based flow sensor (250). According to another aspect, the imaging sensors detect back-reflected light by the particles and/or features, the light emitted by illuminators (250b) in the visible and/ or infrared spectrum. According to yet another aspect, the particles are quantum dot illuminators injected into the fluid mixture, the flow velocity based on a time-of-flight of the quantum dots. The camera-based flow sensor may be rotatable to measure flow velocities at different angular positions of a pipe, rotation provided by rotation of an element of a mobile vessel to which the flow sensor is rigidly coupled.
E21B 47/113 - Locating fluid leaks, intrusions or movements using light radiation
E21B 47/11 - Locating fluid leaks, intrusions or movements using radioactivity
G01P 5/20 - Measuring speed of fluids, e.g. of air stream; Measuring speed of bodies relative to fluids, e.g. of ship, of aircraft by measuring the time taken by the fluid to traverse a fixed distance using particles entrained by a fluid stream
E21B 47/01 - Devices for supporting measuring instruments on drill bits, pipes, rods or wirelines; Protecting measuring instruments in boreholes against heat, shock, pressure or the like
This disclosure herein sets forth embodiments to provide a serological test to detect target analytes that can scale to up to 10,000 or more samples in a single run. This disclosure herein sets forth methods to allow for unique barcoding by using a multi-level barcode scheme that is modular and enables easy detection of multiple analytes, e.g. from SARS-COVID-2, in samples.
The teachings of the present disclosure enable a reduction of the refractive index of a material by incorporating an additive selected from air, vacuum, or an inert gas in the material to turn the material into a material foam that is suitable for use in optical systems. A material foam in accordance with the present disclosure is characterized by a porosity that reduces its refractive index from that of the same material as found in nature. The higher porosity also decreases the density of the material from that of the same material as found in nature. Material foams in accordance with the present disclosure are suitable for use in the low-refractive-index layers of a Bragg mirror.
The disclosure provides systems and methods for generating a signal peptide amino acid sequence using deep learning, where a signal peptide is generated for input protein sequences, amino acids of the protein sequences are tokenized and mapped to a sequence of continuous representations to decode an output signal peptide sequence based on the input protein sequence.
Systems and methods for determining molecular structures based on atomic-orbital- based features are described. Atomic-orbital-based features can be utilized in combination with machine-learning methods to predict accurate properties, such as quantum mechanical energy, of molecular systems.
INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE (INSERM) (France)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) (France)
ECOLE SUPÉRIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS (France)
Inventor
Norman, Sumner L
Maresca, David
Christopoulos, Vasileios
Shapiro, Mikhail
Andersen, Richard A
Abstract
Methods and systems are provided for decoding movement intentions using functional ultrasound (fUS) imaging of the brain. In one example, decoding movement intentions include determining a memory phase of a cognitive state of the brain, the memory phase between a gaze fixation phase and movement execution phase, and determining one or more movement intentions including one or more of intended effector (e.g., hand, eye) and intended direction (e.g., right, left) according to a machine learning algorithm trained to classify one or more movement intentions simultaneously.
Methods of analysis of a sample using hybridization chain reaction (HCR) are provided herein. Some embodiments involve one, two, or all three of the following aspects: 1) repeated signal detection, 2) overlapping binding sites, and 3) catalytic reporter deposition (CARD). Compositions and kits relating to these are also provided. Compositions and kits relating to these are also provided.
patents
