The present application relates to hybridization chain reaction (HCR). In particular, compositions and methods are presented for ultrasensitive molecular detection using HCR signal amplification. Some embodiments and methods involve cooperative probe junctions, reporter-labeled probes, and nonlinear HCR signal amplification.
Disclosed herein include methods, compositions, and kits suitable for use in determining the interaction between proteins and their targets. In some embodiments, the determination of the interaction between proteins and their targets is quantitative and in a high throughput manner.
Catheter comprising altered geometry of a fluid channel to prevent upstream mobility of bacteria, using angled obstacles on the interior of the channel that among other things creates vortices that restrict the mobility. An optimized geometry can be realized by an artificial intelligence algorithm or similar methods based on performance of various configurations of obstacle parameters.
A61M 27/00 - Drainage appliances for wounds, or the like
B33Y 80/00 - Products made by additive manufacturing
G16H 20/40 - ICT specially adapted for therapies or health-improving plans, e.g. for handling prescriptions, for steering therapy or for monitoring patient compliance relating to mechanical, radiation or invasive therapies, e.g. surgery, laser therapy, dialysis or acupuncture
G16H 50/50 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for simulation or modelling of medical disorders
Systems and alloying methods for forming metals are described. Waste materials from various industrial processes and botched master alloy production heats result in numerous byproducts that can form constituent components for the formation of bulk alloys with higher value and more diverse applications. Reusing and upcycling industrial byproducts into material with specific structure and properties result in additional commercial and industrial applications and value.
C22B 7/00 - Working-up raw materials other than ores, e.g. scrap, to produce non-ferrous metals or compounds thereof
B22F 9/08 - Making metallic powder or suspensions thereof; Apparatus or devices specially adapted therefor using physical processes starting from liquid material by casting, e.g. through sieves or in water, by atomising or spraying
6.
A WEARABLE APTAMER NANOBIOSENSOR FOR NON-INVASIVE FEMALE HORMONE MONITORING
Some implementations of the disclosure relate to a wearable biosensor device including: a microfluidic module configured to collect a sweat sample from skin of a user, route the sweat sample to a sensing reservoir that is filled with the sweat sample, and route additional sweat away from the sensing reservoir when the sensing reservoir is filled; and a sensor assembly configured to quantify the biomarker of the sweat sample in the sensing reservoir to determine a concentration of the biomarker present in the sweat sample. The sensor assembly includes a biorecognition interface having a surface functionalized with an aptamer that binds to the biomarker.
A61B 5/145 - Measuring characteristics of blood in vivo, e.g. gas concentration, pH-value
A61B 5/00 - Measuring for diagnostic purposes ; Identification of persons
A61B 5/1477 - Measuring characteristics of blood in vivo, e.g. gas concentration, pH-value using chemical or electrochemical methods, e.g. by polarographic means non-invasive
7.
SYSTEMS AND METHODS FOR DYNAMIC-BACKBONE PROTEIN-LIGAND STRUCTURE PREDICTION WITH MULTISCALE GENERATIVE DIFFUSION MODELS
In some aspects, the present disclosure provides a method for generating a geometrical structure of a binding complex formed between a protein and a ligand. In some embodiments, the method comprises sampling an initial geometrical structure of the binding complex from a geometry prior. In some embodiments, the method comprises denoising, using a machine-learned stochastic differential equation (SDE), the initial geometrical structure to generate the geometrical structure of the binding complex.
8.
POLARIZATION-BASED PLASMONIC SENSORS, SYSTEMS, AND METHODS
Disclosed are plasmonic sensors, and systems and methods related to plasmonic sensors. The plasmonic sensors include polarization-maintaining optical fibers to avoid cross-talk between wanted and unwanted components within a plasmonic response signal. The plasmonic sensors, systems, and methods can involve detecting components within a signal outside of the polarization direction of an excitation signal for reducing issues found in the signal matching the polarization direction of the excitation signal. The plasmonic sensors, systems, and methods use specific lattice arrangements of plasmonic material nanostructures.
G01N 21/77 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
G02B 27/28 - Optical systems or apparatus not provided for by any of the groups , for polarising
The present application relates to hybridization chain reaction (HCR). In particular, the sensitivity of hybridization chain reaction (HCR) signal amplification is combined with two or more fractional-initiator probes and one or more proximity probes able to colocalize a full HCR initiator that will trigger HCR when the targets are in proximity to one another.
in vivoin vivo printing are described. Some implementations of the disclosure relate to a method that includes: obtaining a biopolymer mixture including prepolymer material and a crosslinking agent encapsulated in carrier particles; delivering the biopolymer mixture to a subcutaneous or deep tissue target location of a subject; and transmitting with a bioprinting device, via transcutaneous application, radiation to the subcutaneous or deep tissue target location, the radiation configured to cause the carrier particles to release at least some of the crosslinking agent, the released crosslinking agent configured to cause the prepolymer material to form into a gel or polymeric matrix.
C08F 2/56 - Polymerisation initiated by wave energy or particle radiation by ultrasonic vibrations
C08J 3/24 - Crosslinking, e.g. vulcanising, of macromolecules
C09D 11/101 - Inks specially adapted for printing processes involving curing by wave energy or particle radiation, e.g. with UV-curing following the printing
Provided and described herein are photoactivatable drug conjugates useful for treating extracellular matrix degradation. The photoactivatable drug conjugates generally use targetable delivery of a photosensitizer (e.g., capable of generating singlet oxygen) to sites of extracellular matrix degradation and/or tissue degeneration, wherein photoactivation of the photosensitizer increases extracellular matrix crosslinking and/or tissue strength.
A61K 41/00 - Medicinal preparations obtained by treating materials with wave energy or particle radiation
A61K 47/54 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
A61K 39/395 - Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
12.
ON-CHIP ELECTRICAL COIL AND THREE-DIMENSIONAL ON-CHIP MAGNETIC SENSOR INCLUDING ON-CHIP ELECTRICAL COIL
An on-chip electrical coil includes a semiconductor substrate; a plurality of metal layers disposed on the semiconductor substrate; a plurality of insulator layers disposed on the semiconductor substrate, each insulator layer disposed between a pair of neighboring metal layers to form an alternating arrangement of metal layers and insulator layers; a plurality of metal vias defined in the insulator layers, each metal via electrically connecting a respective pair of neighboring metal layers; and a planar spiral formed by the metal layers and the metal vias, the planar spiral including a plurality of interconnected loops, each loop including two metal wires disposed in respective metal layers, an intra-loop column that electrically connects the two metal wires of a respective loop, and an inter-loop column that electrically connects one of the metal wires of the respective loop to one of the metal wires in a subsequent loop.
G01R 33/00 - Arrangements or instruments for measuring magnetic variables
H01L 23/58 - Structural electrical arrangements for semiconductor devices not otherwise provided for
H01L 23/522 - Arrangements for conducting electric current within the device in operation from one component to another including external interconnections consisting of a multilayer structure of conductive and insulating layers inseparably formed on the semiconductor body
13.
THREE-DIMENSIONAL ON-CHIP MAGNETIC SENSOR FOR OSCILLATING MAGNETIC FIELDS
A three-dimensional on-chip magnetic sensor includes first, second, and third coils. The first and second coils include respective planar spirals formed by metal layers and metal vias. Each planar spiral of the first coil includes first interconnected loops wound about a first axis, where neighboring first planar spirals are electrically connected to each other. Each planar spiral of the second coil includes second interconnected loops wound about a second axis, where neighboring second planar spirals are electrically connected to each other. The third coil has a planar spiral includes third interconnected loops that are wound about a third axis. Each electrically conductive coil is configured to produce a respective electromagnetic force (EMF) induced by an oscillating magnetic field, the respective EMF driving a respective alternating current (AC) through the respective readout circuit. Each readout circuit is configured to detect a respective peak voltage magnitude of the respective AC.
G01R 33/02 - Measuring direction or magnitude of magnetic fields or magnetic flux
G01R 33/00 - Arrangements or instruments for measuring magnetic variables
H01L 23/522 - Arrangements for conducting electric current within the device in operation from one component to another including external interconnections consisting of a multilayer structure of conductive and insulating layers inseparably formed on the semiconductor body
14.
SYSTEMS, METHODS, AND GRAPHICAL USER INTERFACES FOR AUGMENTED REALITY SENSOR GUIDANCE
Systems and methods for real-time environmental sensor data gathering is enhanced using augmented reality, with a virtual target object being presented to the user of the sensor device that guides the user where to move the sensor device next. A combination of pose data for the sensor and data modeling of the sensor data allows for users with minimal training to make optimized environmental readings.
G06F 3/00 - Input arrangements for transferring data to be processed into a form capable of being handled by the computer; Output arrangements for transferring data from processing unit to output unit, e.g. interface arrangements
H04W 4/80 - Services using short range communication, e.g. near-field communication [NFC], radio-frequency identification [RFID] or low energy communication
A cervical elastography system includes an ultrasound imaging system including an ultrasound probe having at least one pressure sensor, a stress calibration system coupled to the ultrasound probe, and a computing device communicatively coupled to the ultrasound imaging system and the stress calibration system. The computing device has a memory, at least one processor, and a module stored in the memory of the computing device and executable by the at least one processor of the computing device to: (1) receive continuously acquired B-mode images of cervix tissue from the ultrasound imaging system; (2) calculate strain on the tissue from the continuously acquired B-mode images; and (3) compute an elasticity of the tissue based on a simultaneous assessment of the actual stress applied to the tissue by the ultrasound probe and the calculated strain on the tissue caused by the stress applied.
An ion focusing device comprising an ion collection element comprising a plurality of N curved electrodes extending around an axis of the ion collection element and an aperture on an axis of the ion collection element. A multipole ion guide adjacent the ion collection element and having a plurality of N elongate electrodes extending parallel to and around an axis of the multipole ion guide, wherein the axis of the multipole ion guide coincides with the axis of the ion collection element, wherein each of the N curved electrodes of the ion collection element are configured to receive a radio frequency, RF, signal having the same phase as an RF signal configured to be applied to at least one elongate electrode of the plurality of elongate electrodes of the multipole ion guide, and further wherein at least two of the N electrodes of the ion collection element are configured to receive RF signals of a different phase.
H01J 49/06 - Electron- or ion-optical arrangements
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
Among the various aspects of the present disclosure is the provision of systems and methods of imaging using photoacoustic computed tomography through an ergodic relay comprisig an acoustic delay line configured to temporally separate one or more initial photoacoustic signals and one or more reflected photoacuostic signals; an acoustic cavity coupled to the acoustic delay line; and an integrated single-element ultrasonic transducer fabricated onto a surface of the acoustic cavity.
Quantum teleportation is essential for many quantum information technologies, including long-distance quantum networks. Using fiber-coupled devices, including state-of-the-art low-noise superconducting nanowire single-photon detectors and off-the-shelf optics, we achieve conditional quantum teleportation of time-bin qubits at the telecommunication wavelength of 1536.5 nm We measure teleportation fidelities of greater than or equal to 90% that are consistent with an analytical model of our system, which includes realistic imperfections. To demonstrate the compatibility of our setup with deployed quantum networks, we teleport qubits over 22 km of single-mode fiber while transmitting qubits over an additional 22 km of fiber. Our systems, which are compatible with emerging solid-state quantum devices, provide a realistic foundation for a high-fidelity quantum Internet with practical devices.
A method for producing a 3D biocomposite structure via additive manufacturing involves mixing a biomass comprising whole photosynthetic microorganisms with a liquid and at least one additive to form a paste, and using a 3D printer to print the paste to produce a wet organic-composite manufacture. The wet manufacture is then dried to produce a dry biocomposite structure. The photosynthetic microorganisms can be cultivated in a growing medium to form the biomass as part of a waste management process. The method can remove more carbon dioxide from the environment via sequestration of carbon in the biomass than it emits. The dry biocomposite structure can have a high flexural modulus and flexural strength and can be coated for a weather-resistant finish and/or assembled with other biocomposite structures to form a larger construction.
B29C 64/165 - Processes of additive manufacturing using a combination of solid and fluid materials, e.g. a powder selectively bound by a liquid binder, catalyst, inhibitor or energy absorber
A sensor including a resonator comprising a nonlinear material comprising a nonlinear susceptibility configured to convert a pump electromagnetic wave (EM) wave to a signal EM wave and an idler EM wave, wherein at least one of the pump EM wave, the signal EM wave and/or the idler EM wave is fed back through the nonlinear material to form one or more resonant EM waves. An actuator coupled to the resonator or a pump path to the resonator, controls at least one of a pump power of the pump EM wave, a detuning of the frequency modes of the resonator relative to one or more frequencies of the resonant EM waves, or a phase matching of the nonlinear material. An output of the resonator outputs one or more output EM waves comprising information about a sample coupled to the resonator.
A transceiver aperture includes an array of pixels. Each of the pixels comprises: a photonic radiator comprising one or more ports; a photonic mixer comprising an Rx input and a local oscillator (LO) input; and an optical mixer comprising a Tx input; an input/output port; a first output; and a second output, wherein the input/output port is coupled to the one or more ports of the photonic radiator and the first output is coupled to the Rx input.
Systems and methods for generating high-pressure hydrogen are described. The hydrogen generation systems include hybrid electrolyzer systems and catalytic compression systems. The systems can directly generate gaseous hydrogen at a pressure of at least 700 bar.
222233 precipitates removal. The system is configured to require the processing of a very small fraction of the total oceanwater intake for the acid-base generation process.
C02F 1/469 - Treatment of water, waste water, or sewage by electrochemical methods by electrochemical separation, e.g. by electro-osmosis, electrodialysis, electrophoresis
C02F 1/24 - Treatment of water, waste water, or sewage by flotation
C02F 1/00 - Treatment of water, waste water, or sewage
C02F 1/42 - Treatment of water, waste water, or sewage by ion-exchange
The present disclosure is directed to novel silicoaluminophosphate (SAPO)-based material with an SAT framework structure (topology type) (SAPO-SAT) that is substantially free of a non-SAPO-SAT phase, as well as the synthesis and use of that SAPO-SAT.
C07C 1/20 - Preparation of hydrocarbons from one or more compounds, none of them being a hydrocarbon starting from organic compounds containing only oxygen atoms as hetero atoms
26.
METHOD AND SYSTEM FOR PRE-TRAINING AND STABILIZING NEUROIMAGING BRAIN-MACHINE INTERFACES
An apparatus and method for a pre-trained brain machine interface based on brain state data is disclosed. An initial session of determining brain state data of a subject during performance of a task at a first time is conducted. The brain state data correlated with task performance are recorded. A pre-training set of the brain state data correlated with the task performance is assembled. A decoder module of the brain machine interface system is pre-trained via the pre-training set of the recorded brain state data to decode movement intentions of the subject correlated with brain state. A current session is conducted at a second time subsequent to the first time. The current session includes the decoder module accepting a brain state data input of the brain of the subject, decoding a brain state output from the brain state data input, and generating a control signal to perform the task based on the determined brain state output.
G06F 3/00 - Input arrangements for transferring data to be processed into a form capable of being handled by the computer; Output arrangements for transferring data from processing unit to output unit, e.g. interface arrangements
Disclosed herein include novel blood-brain barrier (BBB)-crossing receptors on the BBB interface, targeting peptides and derivatives thereof capable of binding to the novel receptors, and related methods of using the receptors to increase the permeability of the BBB and to deliver an agent to a nervous system (e.g., CNS). In some embodiments, the BBB-crossing receptor is LRP6. Disclosed herein also include recombinant adeno-associated viruses (rAAVs) with increased specificity and transduction efficiency across the BBB and related compositions and methods of treating various diseases and conditions.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
28.
SYSTEMS AND METHODS FOR ELECTROCHEMICAL HYDROGEN LOOPING
Systems and methods for electrochemical hydrogen looping cells are described. Generating a pH swing can expedite carbon dioxide capture from oceanwater. Many embodiments implement electrochemical hydrogen looping cells that simultaneously produce acid via anodic hydrogen oxidation and base via cathodic hydrogen evolution to generate a pH change.
Disclosed herein include methods, compositions, and kits suitable for use in signal amplification. There are provided, in some embodiments, protease-based signal amplification modules. Disclosed herein include amplifier proteins comprising a first part of a first protease domain, a first dimerization domain, a first cut site a protease in a protease active state is capable of cutting, a second dimerization domain, a second cut site a protease in a protease active state is capable of cutting, and a first caging domain. Disclosed herein include companion amplifier proteins comprising a second part of a first protease domain, a third dimerization domain, a third cut site a protease in a protease active state is capable of cutting, a fourth dimerization domain, a fourth cut site a protease in a protease active state is capable of cutting, and a second caging domain.
A temperature monitoring system includes a semiconductor member mounted onto the surface of an object having a surface whose temperature is to be monitored. The semiconductor member has a temperature-dependent bandgap with an absorption edge that varies with temperature. A light source is configured to illuminate the semiconductor member with monochromatic light. The monochromatic light has a wavelength equal to an absorption edge wavelength that is associated with the absorption edge when the semiconductor member is at a specified temperature. A detector is configured to receive light reflected from the semiconductor member when illuminated with the monochromatic light such that a surface temperature of the object is at the specified temperature when a change in an amount of reflected light that is received indicates that the wavelength of the monochromatic light is equal to the absorption edge wavelength at the specified temperature.
The present disclosure is directed toward systems and methods for measuring electric current in a multi-wire cable, as well as the apparent and real power associated with an electrical load connected to the cable. A probe comprising an array of small form-factor, high-speed magnetometers is operatively coupled with the cable such that the magnetometers partially surround the cable. Each magnetometer detects the composite magnetic field at its location, and this plurality of measurements is used to generate a magnetic-field map. The contributions of the current flow in each wire are identified by deconvolving the magnetic-field map, enabling their locations to be determined and monitoring of the current and power flow. A sensor included in the probe is used to determine the phase of the applied voltage. The phase difference between the voltage and current is then used to determine the real power dissipation of the load.
G01R 15/12 - Circuits for multi-testers, e.g. for measuring voltage, current, or impedance at will
G01R 15/20 - Adaptations providing voltage or current isolation, e.g. for high-voltage or high-current networks using galvano-magnetic devices, e.g. Hall-effect devices
G01R 19/145 - Indicating the presence of current or voltage
G01R 21/06 - Arrangements for measuring electric power or power factor by measuring current and voltage
G01R 15/14 - Adaptations providing voltage or current isolation, e.g. for high-voltage or high-current networks
G01R 15/18 - Adaptations providing voltage or current isolation, e.g. for high-voltage or high-current networks using inductive devices, e.g. transformers
G01R 19/14 - Indicating direction of current; Indicating polarity of voltage
G01R 19/06 - Measuring real component; Measuring reactive component
G01R 21/08 - Arrangements for measuring electric power or power factor by using galvanomagnetic-effect devices, e.g. Hall-effect devices
32.
LITHIUM-RICH ALUMINUM IRON SULFIDE Li-ION BATTERY CATHODES
w-δxz22. In some embodiments, w may be greater than or equal to 2 and less than or equal to 2.5. In some embodiments, x may be greater than 0 and less than or equal to 0.5. In some embodiments, z may be greater than 0 and less than or equal to 1. In some embodiments, δ may be greater than or equal to 0 and less than w. In some embodiments, the composition may have a net charge of 0.
C01B 17/22 - Alkali metal sulfides or polysulfides
C25B 11/042 - Electrodes formed of a single material
H01M 4/58 - Selection of substances as active materials, active masses, active liquids of polyanionic structures, e.g. phosphates, silicates or borates
H01M 10/0525 - Rocking-chair batteries, i.e. batteries with lithium insertion or intercalation in both electrodes; Lithium-ion batteries
H01M 4/02 - Electrodes composed of, or comprising, active material
33.
METHOD FOR CARDIAC AUSCULTATION USING BLOOD PRESSURE CUFF
The disclosure relates to systems and methods for noninvasively performing cardiac auscultation. In some implementations, a method includes: performing, using a BP cuff of a BP cuff system, a blood pressure measurement of a subject to obtain one or more blood pressure values corresponding to the subject; inflating, based on the one or more blood pressure values corresponding to the subject, the BP cuff to a subject specific pressure value; capturing, using the BP cuff system, while the BP cuff is inflated to the subject specific pressure value, a pulse pressure waveform signal associated with an artery of the subject; obtaining a sound waveform signal associated with a heart valve of the subject by filtering the pulse pressure waveform signal to extract sound components associated with the opening or closing of the heart valve; and analyzing the sound waveform signal to identify characteristics of the heart of the subject.
An integrated photonic architecture for coherent signal generation and processing. This architecture can enhance coherent transceiver performance for many applications, including remote sensing, LiDAR, high-speed data communication, and high performance computing.
G02B 6/12 - Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
35.
HYBRIDIZATION CHAIN REACTION LATERAL FLOW ASSAYS FOR AMPLIFIED DETECTION OF A TARGET IN A SAMPLE
The present disclosure relates to an HCR lateral flow device for amplified detection of a target in a sample and methods of using the same. In some embodiments, the device and methods of use combine the sensitivity of HCR signal amplification with the simplicity of the lateral flow assay format to facilitate user-friendly amplified detection of a target in a sample.
An electrostatic ion trap or an array of electrostatic ion traps are provided having a longitudinal length of no more than 10 mm and/or at least one electrode with a capacitance to ground of no more than 1 pF. First and second sets of planar electrodes may be distributed along the longitudinal axis, at least some of the which are configured to receive an electrostatic potential for confinement of ions received in the space between the first and second sets of planar electrodes. An array may comprise an inlet for receiving an ion beam, configured such that a portion of the ion beam can be trapped in each of the ion traps. Signals indicative of ion mass and charge data may be obtained from multiple electrostatic ion traps in the array. This mass and charge data may be combined for identification of components of a mixture of different analyte ions.
An optical arrangement splits an optical beam into multiple beamlets and focuses each beamlet at a respective line. Each line is defined with reference to a respective spatially-separated location across at least one dimension, such that each beamlet photofragments distinct ions confined at the respective location. Each of the spatially-separated locations may correspond with a respective trapping region of one of an array of ion traps.
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY (USA)
NTT RESEARCH, INC. (USA)
MASSACHUSETTS INSTITUTE OF TECHNOLOGY (USA)
Inventor
Nehra, Rajveer
Yanagimoto, Ryotatsu
Hamerly, Ryan
Ng, Edwin
Marandi, Alireza
Mabuchi, Hideo
Abstract
Methods and systems are presented for using optical parametric amplifiers in various ways that enhance a native quadratic coupling strength so that a photonic component of interest can be measured or otherwise observed without demolishing the component of interest at a system output. For example such components may include a number of signal Bogoliubov excitations, a pump modular quadrature, or a signal quadrature squared.
G06N 10/20 - Models of quantum computing, e.g. quantum circuits or universal quantum computers
B82Y 10/00 - Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
G06N 10/60 - Quantum algorithms, e.g. based on quantum optimisation, or quantum Fourier or Hadamard transforms
G06N 10/80 - Quantum programming, e.g. interfaces, languages or software-development kits for creating or handling programs capable of running on quantum computers; Platforms for simulating or accessing quantum computers, e.g. cloud-based quantum computing
39.
METHODS AND CULTURE MEDIA FOR GENERATING EMBRYOS IN VITRO FROM PLURIPOTENT STEM CELLS
in vitroGATA6SOX17GATA3TFAP2CTFAP2C gene) under a condition in a culture medium allowing the pluripotent stem cells to self-organize into a post-implantation embryo structure. In some embodiments, the pluripotent embryonic stem cells are human pluripotent embryonic stem cells and the generated synthetic embryo is a human embryo.
Disclosed herein include methods and compositions for use in generating synthetic embryos. In some embodiments, the method comprises culturing stem cells that over-express at least one Cadherin.
Methods for utilizing irradiation to selectively transform insulating self-developing resists, such as metal fluorides, into electrically conductive metals are described. The disclosed methods enable the fabrication of electrical components and structures with critical dimensions below 5 nanometers. Selective irradiation induces the conversion of insulating metal fluoride compounds into metals in predefined regions. Examples of applications include miniature wiring, quantum point contacts, miniature electroplating and via-holes fabrication by using fluoride as etching mask.
An apparatus and method for a feature extraction network based brain machine interface is disclosed. A set of neural sensors sense neural signals from the brain. A feature extraction module is coupled to the set of neural sensors to extract a set of features from the sensed neural signals. Each feature is extracted via a feature engineering module having a convolutional filter and an activation function. The feature engineering modules are each trained to extract the corresponding feature. A decoder is coupled to the feature extraction module. The decoder is trained to determine a kinematics output from a pattern of the plurality of features. An output interface provides control signals based on the kinematics output from the decoder.
A complex-wavefront photonic transceiver including a coherent source; a complex transmitter waveform generator programmable to modulate a first portion of the coherent electromagnetic radiation, when received from the coherent source, to form a complex waveform comprising at least a pre-distortion to compensate for, or an adaptive beamforming to determine, a distortion of the complex waveform caused by at least the transceiver or during transmission of the complex waveform to a receiver aperture, the receiver aperture outputting receiver signals in response thereto; and a transmitter aperture for transmitting the complex waveform when received from the complex transmitter waveform generator. The transceiver further includes a receiver processor programmable to determine a phase and amplitude of the complex waveform, when received on the receiver aperture, from a combination of the received signals with a second portion of the electromagnetic radiation.
G01S 17/89 - Lidar systems, specially adapted for specific applications for mapping or imaging
G01S 17/34 - Systems determining position data of a target for measuring distance only using transmission of continuous waves, whether amplitude-, frequency-, or phase-modulated, or unmodulated using transmission of continuous, frequency-modulated waves while heterodyning the received signal, or a signal derived therefrom, with a locally-generated signal related to the contemporaneously transmitted signal
H04N 23/55 - Optical parts specially adapted for electronic image sensors; Mounting thereof
H04N 25/709 - Circuitry for control of the power supply
H01S 3/00 - Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range
G02B 6/12 - Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings of the optical waveguide type of the integrated circuit kind
44.
ORBITAL ANGULAR MOMENTUM SPLITTING WITH VOLUMETRIC META-OPTICS
Methods and devices to split electromagnetic waves across broad bandwidths in correspondence with orbital angular momentum states combined with orthogonal polarization states are disclosed. The described methods can be used in fiber communication and imaging systems. The devices include three-dimensional (3D) scattering structures that can be using existing CMOS processes and direct write lithography techniques. Performance metrics based on the intensity and contrast of the split electromagnetic waves are also disclosed.
Some implementations of the disclosure relate to a wearable biosensor device including an iontophoresis module configured to stimulate production of a sweat sample from skin of a user, the sweat sample including biomarkers; a microfluidic module configured to collect the sweat sample, mix the sweat sample with labeled detection reagents to obtain a mixture including the biomarkers bound to the labeled detection reagents, and route the mixture to a detection reservoir of the microfluidic module; and a sensor assembly including a bioaffinity sensor configured to quantify the biomarkers of the mixture in the detection reservoir to determine a concentration of the biomarkers present in the sweat sample. The bioaffinity sensor includes an electrode functionalized to bind to the biomarkers of the mixture. The bioaffinity sensor can quantify the biomarkers to determine their concentration with a sensitivity on the order of nanomoles or picomoles.
Disclosed herein include antibodies or fragments thereof having specificity to a sarbecovirus spike protein. Also provided are compositions, methods, and kits for using said antibodies or fragments thereof for preventing or treating, for example a coronavirus infection.
Provided herein are methods and systems to selectively deplete a biological sample of host compartments and/or host nucleic acid while enriching the sample microbial compartments and/or related microbial nucleic acid. In addition, provided herein are compositions, methods and systems related to said host depletion and microbial enrichment methods and systems.
Disclosed herein include methods, compositions, and kits suitable for use in winner-take-all neural network computation in mammalian cells. In some embodiments, de novo designed protein heterodimers and engineered viral proteases are combined to implement a synthetic protein circuit that performs winner-take-all neural network computation. The synthetic protein circuit can include modules that compute weighted sums of input protein concentrations through reversible binding interactions, and allow for self-activation and mutual inhibition of protein components using irreversible proteolytic cleavage reactions.
Systems and methods for catalyzed asymmetric bipolar membranes are described. Catalyzed asymmetric bipolar membranes can sustain desired current densities under low operational voltage for prolonged time periods. Catalyzed asymmetric bipolar membranes can be implemented in electrodialysis cells for various applications such as carbon capture.
C02F 1/469 - Treatment of water, waste water, or sewage by electrochemical methods by electrochemical separation, e.g. by electro-osmosis, electrodialysis, electrophoresis
C02F 1/42 - Treatment of water, waste water, or sewage by ion-exchange
C02F 1/461 - Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
A synchronization system for synchronizing photons in a quantum teleportation network. The synchronization system includes a multiplexer combining a clock pulse with a signal photon (carrying a qubit) in an optical fiber, the optical fiber connecting a transmitter node (including the multiplexer) and a receiver node (comprising a demultiplexer). The signal photons have signal wavelengths red shifted as compared to clock wavelengths of the clock pulses. The clock pulses have an intensity below a threshold, such that Raman scattering of the clock pulses by the fiber (shifting the clock wavelengths into the signal wavelengths) is negligible. The receiver node comprises a demultiplexer demultiplexing the one of the signal photons and the one of the clock pulses; a first detector detecting the one of the signal photons; and a second detector detecting the one of the clock pulses.
An implantable medical device to restore brain-controlled movement and sensation after neural injury. The device comprises a brain-computer interface capable of acquiring electrocorticogram (ECoG) signals recorded directly from the surface of the brain and uses the signals to enable direct control of paralyzed muscles, limbs or extremities while simultaneously receiving signals from external sensors and converting them into electrical stimulation patterns for the brain's sensory areas.
Provided herein are HIV immunogens and uses thereof for generating an immune response in a subject. This disclosure further provides a method for treating or preventing a human immunodeficiency type I (HIV-I) infection in a subject using the disclosed HIV immunogens and/or antibodies generated by any of the methods disclosed herein.
3-z13 3 (FX1); wherein Q is a first dopant being a substitute for B in the composition and being one or more elements each aliovalent with respect to B; wherein G is a second dopant being a substitute for S in the composition and being one or more elements each aliovalent with respect to S; wherein z is a number greater than 0 and less than or equal to 0.40, optionally less than or equal to 0.05; and wherein the composition comprises only the first dopant, only the second dopant, or both the first dopant and the second dopant.
A photonic integrated circuit comprising one or more waveguides comprising a second order non-linearity configured to operate on optical pulses each having a pulse length shorter than 1000 optical cycles, as measured at their full width at half maximum. The circuit is configured to generate one or more quantum states carried by one or more of the optical pulses, manipulate one or more of the quantum states, and/or measure one or more of the quantum states.
The present disclosure describes, in part, soluble single-chain dimers (sSCDs) generated from cleavable single-chain trimers (cSCTs), compositions and methods for their production, as well as applications thereof related to characterization of antigen-specific CD8+ T cells and treatments.
A system for coupling a qubit to a register, wherein the system controls application of a protocol comprising a sequence of pulses synchronized with an RF field, the protocol further comprising a timing, a phase, and a duration of each of the pulses comprising a single qubit gate, a period and amplitude of the RF field, and a number of repeats of the sequence, so that application of the protocol controls a coherent spin exchange interaction between a register and a qubit having a zero magnetic dipole moment. The qubit comprises a first spin state and a second spin state both of which have a zero magnetic dipole moment; the register comprises multiple register spins having an energy level structure; and the register spins are indistinguishable so as to be configurable in basis states including a superposition state used for storing the quantum state of the qubit.
The present disclosure is directed toward the simultaneous formation of a plurality of optical elements on a common substrate, where each optical element includes at least one layer having a desired non-uniform-thickness variation. Each such layer is formed such that it includes a plurality of material patterns characterized by the non-uniform thickness variation, where each material pattern is disposed on a different deposition site on the substrate. The material patterns are configured such that adjacent optical elements are separated by a boundary region for facilitating dicing of the substrate into individual optical elements. The non-uniform-thickness layer is formed by direct deposition through a shadow mask that includes a plurality of mask patterns that are either (1) configured to pass material flux in a non-uniform manner or (2) configured to shadow different portions of their respective deposition regions while being moved relative to the substrate.
B05D 5/06 - Processes for applying liquids or other fluent materials to surfaces to obtain special surface effects, finishes or structures to obtain multicolour or other optical effects
B32B 3/08 - Layered products essentially comprising a layer with external or internal discontinuities or unevennesses, or a layer of non-planar form; Layered products essentially having particular features of form characterised by features of form at particular places, e.g. in edge regions characterised by added members at particular parts
B32B 3/30 - Layered products essentially comprising a layer with external or internal discontinuities or unevennesses, or a layer of non-planar form; Layered products essentially having particular features of form characterised by a layer with cavities or internal voids characterised by a layer formed with recesses or projections, e.g. grooved, ribbed
H01L 21/308 - Chemical or electrical treatment, e.g. electrolytic etching using masks
B05D 7/24 - Processes, other than flocking, specially adapted for applying liquids or other fluent materials to particular surfaces or for applying particular liquids or other fluent materials for applying particular liquids or other fluent materials
H01L 21/68 - Apparatus specially adapted for handling semiconductor or electric solid state devices during manufacture or treatment thereof; Apparatus specially adapted for handling wafers during manufacture or treatment of semiconductor or electric solid state devices or components for positioning, orientation or alignment
An adaptive LiDAR includes, in part, a transmitter and a receiver. The transmitter includes, in part, an array of N radiators, and a transmitter control block adapted to control an aperture of the transmitter. The receiver includes, in part, an array of T receive elements, and a receiver control block adapted to control a scan rate and resolution of the receiver. M and T are integers greater than one.
A macromolecular mechanophore platform that undergoes a mechanically gated chromogenic reaction in the presence of an extrinsic small molecule developing reagent is described, along with methods of synthesis and use thereof in different scenarios. The platform comprises a hetero-Diels Alder adduct embedded into a polymer, wherein the hetero-Diels Alder adduct masks an activated furan, and wherein the activated furan is released from the platform upon application of mechanical force, to react with the developing reagent comprising a secondary amine of choice to produce colored species with photoswitch capabilities. The platform can be used in solution or in solid state, and different colors can be obtained for the same platform, since the color of the generated product is determined by the choice of the developing reagent. Incorporating the platform into polymeric materials enables multicolor mechanical lithography by sequential application of mechanical force and different developing reagents.
G03F 7/105 - Photosensitive materials - characterised by structural details, e.g. supports, auxiliary layers having substances, e.g. indicators, for forming visible images
G03F 7/00 - Photomechanical, e.g. photolithographic, production of textured or patterned surfaces, e.g. printed surfaces; Materials therefor, e.g. comprising photoresists; Apparatus specially adapted therefor
G03F 7/32 - Liquid compositions therefor, e.g. developers
C08F 8/32 - Introducing nitrogen atoms or nitrogen-containing groups by reaction with amines
Disclosed herein include novel blood-brain barrier (BBB)-crossing receptors on the BBB interface, targeting peptides and derivatives thereof capable of binding to the novel receptors, and related methods of using the receptors to increase the permeability of the BBB and to deliver an agent to a nervous system (e.g., CNS). In some embodiments, the BBB-crossing receptor is carbonic anhydrase IV. Disclosed herein also include recombinant adeno-associated viruses (rAAVs) with increased specificity and transduction efficiency across the BBB and related compositions and methods of treating various diseases and conditions.
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
C07K 16/40 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against enzymes
An optica I -th in -film structure comprises a low-index optical thin film consisting essentially of co-deposited Barium Fluoride and a secondary fluoride compound, and a high-index optical thin film.
G01D 5/26 - Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using optical means, i.e. using infrared, visible or ultraviolet light
Disclosed herein include methods, compositions, and kits suitable for use in generating barcoded detection particles. Each barcoded detection particle can comprise a particle associated with an antigen-binding protein and a plurality of barcoding oligonucleotides. The plurality of barcoding oligonucleotides can comprise a first ligand. The particle can comprise a second ligand. The plurality of barcoding oligonucleotides can be associated with the particle via a multivalent binding agent comprising two or more binding moieties capable of binding the first ligand and/or the second ligand. There are provided, in some embodiments, methods for detecting interactions between nucleic acid molecules and proteins of interest. Methods for detecting interactions between ribonucleic acid molecules and RNA-binding proteins (RBPs) are also provided herein.
C08L 23/18 - Homopolymers or copolymers of hydrocarbons having four or more carbon atoms
C08L 101/00 - Compositions of unspecified macromolecular compounds
C08F 255/08 - Macromolecular compounds obtained by polymerising monomers on to polymers of hydrocarbons as defined in group on to polymers of olefins having four or more carbon atoms
A61B 17/00 - Surgical instruments, devices or methods, e.g. tourniquets
64.
HIGH THROUGHPUT MICROCRYSTAL SOAKING FOR STRUCTURAL ANALYSIS OF PROTEIN-LIGAND INTERACTIONS HIGH THROUGHPUT MICROCRYSTAL SOAKING FOR STRUCTURAL ANALYSIS OF PROTEIN-LIGAND INTERACTIONS
B01J 20/28 - Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
B01D 53/02 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography
66.
SUPPRESSION OF NON-SPECIFIC SIGNALS BY EXONUCLEASES IN FISH EXPERIMENT
Disclosed herein is a composition for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples. Also disclosed herein, is a kit for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples. Also disclosed herein, is a method for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples.
in vitroin vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.
Systems and methods for a self-powered wireless wearable sensor system include a photovoltaic (PV) panel array, used as a power source for a wearable sensor. The PV panel array may be attached to an area of the human body exposed to a light source. Exposure to a light source may generate an electric field and power a wearable device sufficiently to support data transmission and continuous monitoring. An integrated self-powered wireless wearable sensor system may include a microfluidic sweat sensor patch that may be connected to lower-power wireless sensor circuitry for regulating power efficiently and may be powered by the PV panel array.
B01D 53/02 - Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography
B01D 46/00 - Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
70.
METHOD OF MAPPING SPATIAL DISTRIBUTIONS OF CELLULAR COMPONENTS
This disclosure herein sets forth embodiments for a method for mapping the spatial distribution of one or more cellular components or cellular interactions by using barcodes to identify a cell or cellular component in the one or more cells, localizing, detecting, and mapping the spatial distribution of the one or more cellular components. The disclosure herein sets forth method to allow the mapping of spatial distributions of cellular components both intracellularly or intercellularly.
Disclosed herein include methods, compositions, and kits suitable for use in sorting a population of cells. In some embodiments, the method comprises flowing a fluid sample comprising a population of cells through a microfluidic channel. The population of cells can be configured to express gas vesicles (GVs) in a context-dependent manner. The expression of GVs within a cell can increase the compressibility (β) and reduce the density (ρ) of said cell, thereby modulating the acoustic contrast (Φ) of said cell relative to the fluid in the microfluidic channel. The method can comprise applying ultrasound to the microfluidic channel. Applying ultrasound can generate acoustic standing wave(s) in the microfluidic channel, thereby positioning pressure antinode(s) in the microfluidic channel.
Disclosed herein include antibodies or fragments thereof having specificity to a sarbecovirus spike protein receptor binding domain. Also provided are compositions, methods, and kits for isolating and using said antibodies or fragments thereof for preventing or treating, for example a coronavirus infection.
Systems and methods for an electronic skin based robotic system including a robotic interface and a human subject are provided. An e-skin may be applied to the robotic interface. The e-skin applied to the robotic interface may include a plurality of physicochemical sensors. An e-skin may also be applied to the human subject. The e-skin may include electrodes for sensing muscular contractions associated with hand and arm movements as well as electrodes for stimulation. Machine learning techniques may enable decoding of signals to control the robotic hand and arm. The robotic hand and arm may be controlled to approach unknown compounds that may be hazardous. The sensors making up the physicochemical sensors on the e-skin on the robotic hand and arm may include tactile, pressure, temperature, and chemical sensors, as well as other useful sensors. These sensors may enable detection of explosives, organophosphates, pathogenic proteins, and other hazardous compounds.
G01L 1/20 - Measuring force or stress, in general by making use of electrokinetic cells, i.e. liquid-containing cells wherein an electrical potential is produced or varied upon the application of stress
G01L 5/22 - Apparatus for, or methods of, measuring force, work, mechanical power, or torque, specially adapted for specific purposes for measuring the force applied to control members, e.g. control members of vehicles, triggers
74.
NON-INVASIVE METHOD AND DEVICE FOR CONTINUOUS SWEAT INDUCTION AND COLLECTION
Systems and methods for a microfluidic biosensor patch and health monitoring system may include an iontophoresis module, a multi-inlet microfluidic sweat collection and sampling module, and a molecularly imprinted polymer (MIP) organic compound sensor module. An iontophoresis module may provide for stimulation of a biofluid sample. A biofluid may be a sweat sample. Stimulation may be achieved via electrostimulation and/or application of a stimulating agent. A microfluidic sweat collection and sample module may include several adhesive layers with carefully designed inlets, channels, a reservoir, and an outlet for the efficient collection and sampling of biofluid. A MIP sensor module may quickly and accurately identify concentrations of key metabolites present in a biofluid sample which may indicate certain health conditions.
A61B 5/145 - Measuring characteristics of blood in vivo, e.g. gas concentration, pH-value
G16H 50/80 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for detecting, monitoring or modelling epidemics or pandemics, e.g. flu
G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
G16H 10/20 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for electronic clinical trials or questionnaires
Systems and methods for a smart bandage for monitoring and treating wounds are provided herein. The smart bandage may be a smart, wearable, flexible multi-layer substrate that may include a system that can monitor wound characteristics, perform autonomous bioanalysis of wound characteristics to determine wound treatment plans in a non-invasive method, perform drug delivery or antimicrobial agent release to treat and prevent infections, and promote healing by electrical stimulation. The smart bandage may be equipped with a wireless network that can communicate with users, such as patients and medical providers, directly about a patient's wound condition and provide for on-demand wound treatment.
Described herein are compositions and kits comprising recombinant adeno-associated viruses (rAAVs) with increased transduction enrichment in the heart and/or skeletal muscle and, in some cases, reduced transduction in the liver. The rAAV compositions described herein encapsidate a transgene, such as a therapeutic nucleic acid. Gene therapy using the rAAVs is described. Also described are methods of treating diseases and conditions of the heart and/or skeletal muscle.
A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermen
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
C07K 14/00 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
77.
SYSTEM FOR OPTICALLY ANALYZING A TEST SAMPLE AND METHOD THEREFOR
The present disclosure is directed toward measurement systems capable of optical analysis of a test sample. Embodiments in accordance with the present disclosure include a sample holder having a plurality of projections that extend from a planar surface, where the projections and planar surface collectively define an open sample-collection surface that enables an interrogation signal direct access to the test sample. The projections can be dimensioned and arranged to collectively define a geometric anti-reflection surface that is substantially non-reflective for the interrogation signal even at large angles of incidence. In some embodiments, the sample holder is configured as a reflective element that enables multiple passes of the interrogation signal through the test sample. In some embodiments, the sample holder is configured as a transmissive element. In some embodiments, the projections themselves are reflective.
G01N 21/3577 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
G01N 21/01 - Arrangements or apparatus for facilitating the optical investigation
G01N 21/35 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
The present disclosure provides engineered enzymes and methods for preparing tyrosine and tyrosine analogs from a donor amino acid and phenols in a single step. Also disclosed are methods to engineer enzymes having such 'tyrosine synthase' activity, starting from the beta-subunit of a tryptophan synthase (TrpB).
GOTTFRIED WILHELM LEIBNIZ UNIVERSITÄT HANNOVER (Germany)
HELMHOLTZ ZENTRUM MÜNCHEN - DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) (Germany)
Inventor
Plettenburg, Oliver
Ahlbrecht, Christin
Adalian, Dvin
Scherer, Axel
Madero, Xiomara Linnette
Chen, Samson
Jilani, Muhammad Musab
Abstract
Methods and systems are described for fabricating thin hydrogel layers on biosensors by a drop-spin method, which includes placing a drop of the hydrogel on the electrode, spinning the wafer at high speed in a vacuum, and heating the wafer to cure. One and multilayer sensors can be fabricated in this way, by adding layers of hydrogel or metal.
Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic macrophages through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow macrophages to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Genetic circuits capable of inducing expression of a payload upon thermal stimulation are provided. There are provided, in some embodiments, heat-inducible macrophages and methods of using are provided.
C07K 14/47 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from humans from vertebrates from mammals
A61K 35/15 - Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
Disclosed herein include methods, compositions, and kits suitable for use in calcium imaging. There are provided, in some embodiments, Ca2+-sensing GvpC proteins. Disclosed herein include Ca2+-sensing gas vesicles (GVs) comprising Ca2+-sensing GvpC proteins. In some embodiments, the Ca2+-sensing GvpC protein is capable of undergoing a first allosteric conformational change upon the Ca2+-binding domain binding Ca2+that causes the Ca2+-sensing GV to change from a GV stiff state to a GV soft state. One or more of the mechanical, acoustic, surface, and magnetic properties of a Ca2+-sensing GV can differ between the GV soft state and the GV stiff state.
Disclosed herein include a photoactivatable vibrational probe, when photoactivated, capable of forming a Raman probe for detection by Raman scattering. In some embodiments, the photoactivatable vibrational probe has a structure of Formula I. Disclosed herein also includes methods of using the photoactivatable vibrational probe for live-cell multiplexed imaging and tracking.
Disclosed herein include methods, compositions, and kits suitable for use in imaging of in situ gene expression. There are provided, in some embodiments, viral vector compositions. Disclosed herein includes a single viral vector comprising one or more gas vesicle assembly (GV A) gene(s) encoding one or more GV A protein(s), and one or more gas vesicle structural (GVS) gene(s) encoding one or more GVS protein(s). The one or more GV A protein(s) and the one or more GVS protein(s) can be capable of forming gas vesicles (GVs) upon expression in a cell.
Systems and methods for identifying the components of a long-chain molecule by making electrical measurements from fabricated nanoscale electrodes as the molecule moves down a narrow microfluidic channel. The channel can be along the surface of a chip, through a chip, or both.
Disclosed herein include compositions and kits comprising recombinant adeno-associated viruses (rAAVs) with tropisms to the central nervous system, the peripheral nervous system, and/or the lung, with increased specificity and transduction efficiency. Also described include methods of treating various diseases and conditions using the rAAVs.
C07K 14/005 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 11/00 - Drugs for disorders of the respiratory system
A61P 25/00 - Drugs for disorders of the nervous system
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
87.
SYSTEMS AND METHODS FOR GAS-LIQUID CONTACTORS FOR RAPID CARBON CAPTURE
Disclosed herein include methods, compositions, and kits suitable for use in the delivery of polyribonucleotides and circuits. There are provided, in some embodiments, RNA exporter proteins comprising an RNA-binding domain, a membrane-binding domain, and an interaction domain capable of nucleating self-assembly. Disclosed herein include polynucleotides encoding cargo RNA molecule(s). In some embodiments, a plurality of RNA exporter proteins are capable of self-assembling into lipid-enveloped nanoparticles (LNs) secreted from a sender cell in which the RNA exporter proteins are expressed, thereby generating a population of LNs comprising a fusogen and exported cargo RNA molecule(s).
Disclosed herein include methods, compositions, and kits suitable for use in the measurement of the states of living cells across time. There are provided, in some embodiments, RNA exporter proteins comprising an RNA-binding domain, a membrane-binding domain, and an interaction domain capable of nucleating self-assembly. Disclosed herein include polynucleotides encoding reporter RNA molecule(s). In some embodiments, a plurality of RNA exporter proteins are capable of self-assembling into lipid-enveloped nanoparticles (LNs) secreted from a reporter cell in which the RNA exporter proteins are expressed, thereby generating a population of LNs comprising exported reporter RNA molecule(s).
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
Systems and methods are provided for a non-invasive high resolution pressure pulse waveform measurement system. The system may include a blood pressure cuff, an air pump to inflate the blood pressure cuff to specific pressure levels, high resolution pressure sensors configured to perform high sensitivity signal acquisition at a specified pressure level, high range pressure sensors configured to measure an absolute reference for the signal and to calibrate the signal, pneumatic tubing connecting the air pump and sensors with the cuff, and a hydrodynamic filter configured as an input to a reference port of the high resolution pressure sensor. The hydrodynamic filter may be configured to transmit only mean pressure by attenuating a selected frequency range of the signal.
A61B 5/02 - Measuring pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography; Heart catheters for measuring blood pressure
Systems and methods disclosed herein relate to the detection of irregular particles in a blood flow based on a determined relative speed of a particle suspended in a blood flow and/or other properties of a particle suspended in a blood flow including a particle's relative position within a blood vessel and a particles tendency to cluster with other particles suspended in a blood flow. Based on a determined relative speed and/or other relevant factors, the properties of irregular particles may also be measured, including the size, shape, and frequency of irregular particles in a blood flow. Machine learning techniques may be employed to determine patterns for the behavior of irregular particles suspended in a blood flow. These patterns may correspond to particular health risks and conditions.
Disclosed herein include adeno-associated virus (AAV) acoustic targeting peptides. An AAV comprising the AAV acoustic targeting peptide can exhibit increased transduction at site(s) of focused ultrasound blood-brain barrier opening (FUS-BBBO), increased neuronal tropism, and diminished transduction of peripheral organs. Disclosed herein include recombinant adeno-associated virus (rAAV) comprising an AAV acoustic targeting peptide disclosed herein. Also provided herein include methods of delivering an agent to one or more target brain region(s) of a subject.
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61K 9/00 - Medicinal preparations characterised by special physical form
A61P 25/00 - Drugs for disorders of the nervous system
93.
STOICHIOMETRIC EXPRESSION OF MESSENGER POLYCISTRONS
Disclosed herein include methods, compositions, and kits enabling expression of multiple proteins from a single mRNA with a predetermined stoichiometry. There are provided, in some embodiments, nucleic acid compositions comprising a promoter operably linked to a polynucleotide comprising a first nucleic acid unit encoding first unit payload protein(s) and a second nucleic acid unit encoding second unit payload protein(s). The first nucleic acid unit and the second nucleic acid unit can each comprise a 3' engineered translation initiation site (eTIS) comprising a three-nucleotide tunable element immediately upstream of a start codon. The eTIS of each of the first nucleic acid unit and the second nucleic acid unit can be configured to achieve a predetermined stoichiometry of the first unit payload protein(s) and the second unit payload protein(s) in a cell or cell-like environment.
C12N 15/63 - Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12N 15/67 - General methods for enhancing the expression
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
C07K 14/00 - Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
94.
STAIN-FREE DETECTION OF EMBRYO POLARIZATION USING DEEP LEARNING
Disclosed herein include systems, devices, and methods for detecting embryo polarization from a 2D image generated from a 3D image of an embryo that is not fluorescently labeled using a convolutional neural network (CNN), e.g., deep CNN.
Provided herein are methods and compositions for inhibiting p97, for the treatment of a motor neuron disease in a subject, or a symptom thereof. Upon treatment, the motor neuron disease, or a symptom thereof is reduced in the subject.
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
A61K 31/4439 - Non-condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
A61K 31/519 - Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
A self-deployable antenna and/or antenna array that is made up of one or more antenna elements. Each of the antenna elements has a structural base that supports portions of the antenna and can be positioned between a stored configuration for compaction and a deployed configuration for transmitting. The antenna elements and structural base can be part of a base substrate that provides a base support for the antenna and/or antenna array to be compacted and deployed.
H01Q 1/38 - Structural form of radiating elements, e.g. cone, spiral, umbrella formed by a conductive layer on an insulating support
H01Q 9/28 - Conical, cylindrical, cage, strip, gauze or like elements having an extended radiating surface; Elements comprising two conical surfaces having collinear axes and adjacent apices and fed by two-conductor transmission lines
H01Q 19/30 - Combinations of primary active antenna elements and units with secondary devices, e.g. with quasi-optical devices, for giving the antenna a desired directional characteristic using a secondary device in the form of two or more substantially straight conductive elements the primary active element being centre-fed and substantially straight, e.g. Yagi antenna
Disclosed herein include methods, compositions, and kits suitable for use in vaccination. There are provided, in some embodiments, nucleic acid compositions (e.g., mRNA vaccine, DNA vaccine) comprising a polynucleotide encoding a fusion protein. The fusion protein can comprise an antigenic polypeptide (AP) and an endosomal sorting complex required for transport (ESCRT)-recruiting domain (ERD). A plurality of fusion proteins can be capable of self-assembling into an enveloped nanoparticle (ENP) secreted from a cell in which the fusion proteins are expressed. There are provided, in some embodiments, populations of ENPs.
Disclosed herein are methods for generating an ratiometric symbol for sequential hybridization barcoding for multiplexed Fluorescence In Situ Hybridization (FISH). Also, the disclosure sets forth methods, in addition to using the same, and other solutions to problems in the relevant field.
A structure having a tunable modulus of bending (flexibility) composed of interlocking geometric particles arranged such that an external pressure/force causes them to jam together, increasing the stiffness of the overall structure. Methods for creating the external pressure can include the use of an envelope around the structure that can be evacuated of air.
B29C 43/10 - Isostatic pressing, i.e. using non-rigid pressure-exerting members against rigid parts or dies
B29C 64/153 - Processes of additive manufacturing using only solid materials using layers of powder being selectively joined, e.g. by selective laser sintering or melting
Disclosed herein is a composition for linked amplification tethered with exponential radiance for signal amplification. Also disclosed herein, is a kit for linked amplification tethered with exponential radiance for signal amplification. Also disclosed herein, is a method linked amplification tethered with exponential radiance for signal amplification.
patents
