Abstract

The present disclosure is drawn to loop-mediated isothermal amplification (LAMP) reaction assemblies including a substantially hygroscopic agent free LAMP reagent mixture in combination with a solid-phase reaction medium. The present disclosure also includes systems for a chromatic LAMP analysis including a substantially non-reactive solid phase reaction medium, and a non-interfering reagent mixture. The present disclosure also includes solid phase LAMP reaction mediums comprising a substrate, an adhesive layer disposed on the substrate, a reaction layer disposed on the adhesive layer, and a spreading layer disposed on the reaction layer. The present disclosure also includes methods of testing for a presence of a target nucleotide sequence including providing a biological sample, and dispensing the sample into a test environment having a solid phase reaction medium in combination with a LAMP reagent mixture and a pH sensitive dye.

IPC Classes  ?

• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates

Abstract

The present disclosure is drawn to compositions and methods for loop-mediated isothermal amplification (LAMP) analysis utilizing a pH-dependent output signal. The composition can comprise a pH sensitive dye, and a plurality of non-interfering LAMP reagents. The method can comprise providing an assembly of a solid phase medium and a composition, depositing a biological sample onto the solid phase medium, and heating the assembly to an isothermal temperature sufficient to facilitate a LAMP reaction.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Abstract

The present disclosure is drawn to compositions, methods, and systems for loop-mediated isothermal amplification (LAMP) analysis on a solid phase medium. The composition can comprise one or more target primers, a DNA polymerase, and a re-solubilization agent. The composition can be substantially free of non-pH sensitive agents capable of discoloring the solid phase medium. The method can comprise providing an assembly of a solid phase medium, depositing a biological sample onto the solid phase medium, and heating the assembly to an isothermal temperature sufficient to facilitate a LAMP reaction. The system can comprise a composition and a solid phase medium on to which the composition is deposited.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

A receiver for cancelling strong signals from combined weak and strong signals includes: a first circuitry for inputting a weak and strong signal as an input; a parametric cancellation circuit for inputting a representation of the strong signal and an output of the first circuitry to produce a cancellation signal; a second circuitry electrically coupled to the parametric cancellation circuit for inputting the cancellation signal to produce a modulated output; a demodulator electronically coupled to the second circuitry for demodulating the modulated output to produce a demodulated output and an error signal, where the demodulated output is the data contained in the weak signal; and an adaptation logic circuit for inputting the representation of the strong signal, the demodulated output and the error signal to adaptively produce parameters for the parametric cancellation circuit. The parametric cancellation circuit further inputs the error signal and the parameters to produce the cancellation signal.

IPC Classes  ?

• H04B 1/10 - Means associated with receiver for limiting or suppressing noise or interference

Abstract

An electro-optical system for exchanging quantum information between optical qubits and including a superconductive microwave cavity; an electro-optical material; a superconductive qubit circuit formed on the electro-optical material including a superconductive qubit; a dipole antenna, formed on the electro-optical material for directly coupling the superconductive qubit to the superconductive microwave cavity; an optical input for receiving input optical photons; a microwave input for receiving input microwave photons; and an optical output for outputting modulated optical photons, wherein a frequency and a phase of the optical photon is modulated with a state of the superconducting qubit by the dipole antenna.

IPC Classes  ?

• B82Y 10/00 - Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• B82Y 20/00 - Nanooptics, e.g. quantum optics or photonic crystals
• G06E 3/00 - Devices not provided for in group , e.g. for processing analogue or hybrid data