PCR KIT-OF-PARTS, METHOD AND SYSTEM
| Register | WIPO Patent |
|---|---|
| Application Number | US2021043118 |
| Publication Number | 2023/009099 |
| Status | In Force |
| Filing Date | 2021-07-26 |
| Publication Date | 2023-02-02 |
| Owner | HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P. (USA) |
| Inventor |
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Abstract
A PCR kit-of-parts is described. The kit-of-parts comprises a first oligonucleotide bound to a bead by a cleavable linker; a second oligonucleotide bound to a bead by a cleavable linker; and an enzyme bound to a bead by a cleavable linker; wherein the first and second oligonucleotide form an oligonucleotide pair complementary to a nucleic acid of interest and the enzyme is capable of extending nucleic acid strands; and each cleavable linker is independently selected from a photocleavable linker and a thermally cleavable linker. Also described is a method of performing PCR and a PCR system.IPC Classes ?
- C12Q 1/686 - Polymerase chain reaction [PCR]
- C12Q 1/6844 - Nucleic acid amplification reactions
- C12M 1/38 - Temperature-responsive control
- G01N 21/63 - Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N 21/71 - Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light thermally excited
- G01N 21/84 - Systems specially adapted for particular applications